Binder CB 170 User manual

Issue 06/2018 Art. No. 7001-0326
Operating Manual
CB (E7)
CO2Incubators
CO2Incubators with O2Control
CO2Incubators with Active Humidification
with sterilizable NDIR sensor system for CO2
and microprocessor program controller MB2
Model Model version Voltage Equipment Art. no.
CB 170 CB170-230V 200-230 V 9040-0131, 9140-0131
CB 170 CB170-230V-O 200-230 V O2control 0-20 vol.-% 9040-0132, 9140-0132
CB 170 CB170-230V-F 200-230 V Active humidification 9040-0133, 9140-0133
CB 170 CB170-230V-
FO 200-230 V Active humidification
O2control 0-20 vol.-% 9040-0134, 9140-0134
CB 170-UL CB170-120V 100-120 V 9040-0139, 9140-0139
CB 170-UL CB170-120V-O 100-120 V O2control 0-20 vol.-% 9040-0140, 9140-0140
CB 170-UL CB170-120V-F 100-120 V Active humidification 9040-0141, 9140-0141
CB 170-UL CB170-120V-
FO 100-120 V Active humidification
O2control 0-20 vol.-% 9040-0142, 9140-0142
BINDER GmbH
Address: Post office box 102, 78502 Tuttlingen, Germany Phone: [phone]
Fax: [phone] Internet: http://www.binder-world.com
E-mail: [e-mail] Service Hotline: [phone]
Service Fax: [phone] Service E-Mail: [e-mail]
Service Hotline USA: [phone] or [phone] x3
Service Hotline Asia Pacific: [phone] or [phone]
Service Hotline Russia and CIS: [phone]

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Contents
1. SAFETY..................................................................................................................7
1.1 Legal considerations...........................................................................................................................7
1.2 Structure of the safety instructions......................................................................................................7
1.2.1 Signal word panel .....................................................................................................................7
1.2.2 Safety alert symbol...................................................................................................................8
1.2.3 Pictograms................................................................................................................................8
1.2.4 Word message panel structure.................................................................................................9
1.3 Localization / position of safety labels on the chamber ....................................................................10
1.4 Type plate .........................................................................................................................................11
1.5 General safety instructions on installing and operating the CO2incubator ......................................12
1.6 Precautions when working with gases..............................................................................................13
1.7 Precautions when handling gas cylinders.........................................................................................15
1.8 Intended use .....................................................................................................................................16
1.9 Measures to prevent accidents.........................................................................................................17
1.10 Resistance of the humidity sensor against harmful substances.......................................................18
2. CHAMBER DESCRIPTION..................................................................................19
2.1 Chamber overview............................................................................................................................20
2.2 Inner chamber...................................................................................................................................21
2.3 Control panel on the rear of the chamber.........................................................................................22
2.4 Instrument panel ...............................................................................................................................23
3. COMPLETENESS OF DELIVERY, TRANSPORTATION, STORAGE, AND
INSTALLATION....................................................................................................23
3.1 Unpacking, and checking equipment and completeness of delivery................................................23
3.2 Guidelines for safe lifting and transportation.....................................................................................24
3.3 Storage..............................................................................................................................................24
3.4 Location of installation and ambient conditions................................................................................25
4. INSTALLATION AND CONNECTIONS ...............................................................28
4.1 Shelves..............................................................................................................................................28
4.2 Permadry™ water pan......................................................................................................................28
4.3 Connecting the O2sensor (chamber with O2control).......................................................................29
4.4 Gas connections ...............................................................................................................................30
4.4.1 Connection of the CO2gas cylinder........................................................................................31
4.4.2 Connection of the O2gas cylinder (chamber with O2control with optional alternative control
range 10 up to 95 vol.-% O2) ..................................................................................................32
4.4.3 Connection of the N2gas cylinder (chamber with O2control).................................................33
4.4.4 Connecting the gas hose to the chamber rear (for CO2, O2, N2)............................................34
4.4.5 Gas cylinder connection kits (option)......................................................................................35
4.5 Water supply for the chamber with active humidification..................................................................36
4.5.1 Types of suitable water quality ...............................................................................................36
4.5.2 Water supply installation.........................................................................................................37
4.5.3 BINDER Pure Aqua Service (option)......................................................................................37
4.6 Electrical connection.........................................................................................................................38
4.7 Handling and aligning the divided inner door, gas proof (optional equipment).................................39
5. FUNCTIONAL OVERVIEW OF THE MB2 CHAMBER CONTROLLER...............40
5.1 Operating functions in normal display...............................................................................................41
5.2 Display views: Normal display, program display, chart-recorder display..........................................42
5.3 Controller icons overview..................................................................................................................43
5.4 Operating modes...............................................................................................................................45
5.5 Controller menu structure..................................................................................................................46
5.5.1 Main menu..............................................................................................................................47
5.5.2 “Settings” submenu.................................................................................................................48
5.5.3 “Service” submenu..................................................................................................................48

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5.6 Principle of controller entries.............................................................................................................49
5.7 Performance during and after power failures....................................................................................49
5.8 Performance when opening the door................................................................................................50
6. START UP............................................................................................................50
6.1 Turning on the chamber....................................................................................................................50
6.2 Controller settings upon start up.......................................................................................................51
6.3 Entering the altitude of the installation site .......................................................................................52
6.4 Factory settings.................................................................................................................................52
6.5 Equilibration time...............................................................................................................................53
6.6 Operating the DuoDoor™ door lock..................................................................................................53
6.7 Required gas supply for the chamber with O2control.......................................................................54
6.7.1 Hypoxic control range 0.2 vol.-% up to 20 vol.- % O2(regular)..............................................54
6.7.2 Alternative control range 10 vol.-% up to 95 vol.-% (option)..................................................54
6.7.3 Operation without O2control...................................................................................................54
6.8 Activating / deactivating CO2, O2and humidity control.....................................................................55
6.9 Humidity control of the Permadry™ system......................................................................................56
7. SET-POINT ENTRY IN “FIXED VALUE” OPERATING MODE...........................57
7.1 Set-point entry through the “Setpoints” menu...................................................................................58
7.2 Direct setpoint entry via Normal display............................................................................................59
7.3 Special controller functions via operation lines.................................................................................60
7.4 Safety instructions for setting high gas concentrations.....................................................................61
8. TIMER PROGRAM: STOPWATCH FUNCTION ..................................................62
8.1 Starting a timer program ...................................................................................................................62
8.1.1 Performance during program delay time ................................................................................62
8.2 Stopping a running timer program ....................................................................................................63
8.2.1 Pausing a running timer program ...........................................................................................63
8.2.2 Cancelling a running timer program........................................................................................63
8.3 Performance after the end of the program........................................................................................63
9. TIME PROGRAMS...............................................................................................64
9.1 Starting an existing time program.....................................................................................................64
9.1.1 Performance during program delay time ................................................................................65
9.2 Stopping a running time program......................................................................................................65
9.2.1 Pausing a running time program.............................................................................................65
9.2.2 Cancelling a running time program.........................................................................................65
9.3 Performance after the end of the program........................................................................................65
9.4 Creating a new time program............................................................................................................66
9.5 Program editor: program management.............................................................................................66
9.5.1 Deleting a time program .........................................................................................................67
9.6 Section editor: section management.................................................................................................68
9.6.1 Add a new program section....................................................................................................69
9.6.2 Copy and insert or replace a program section........................................................................69
9.6.3 Deleting a program section.....................................................................................................70
9.7 Value entry for a program section.....................................................................................................71
9.7.1 Section duration......................................................................................................................71
9.7.2 Set-point ramp and set-point step...........................................................................................72
9.7.3 Special controller functions via operation lines.......................................................................74
9.7.4 Setpoint entry..........................................................................................................................75
9.7.5 Tolerance range......................................................................................................................75
9.7.6 Repeating one or several sections within a time program......................................................76
9.7.7 Saving the time program.........................................................................................................77
10. WEEK PROGRAMS.............................................................................................78
10.1 Starting an existing week program....................................................................................................78
10.2 Cancelling a running week program .................................................................................................78
10.3 Creating a new week program..........................................................................................................79

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10.4 Program editor: program management.............................................................................................80
10.4.1 Deleting a week program........................................................................................................81
10.5 Section editor: section management.................................................................................................82
10.5.1 Add a new program section....................................................................................................83
10.5.2 Copy and insert or replace a program section........................................................................83
10.5.3 Deleting a program section.....................................................................................................84
10.6 Value entry for a program section.....................................................................................................84
10.6.1 Set-point ramp and set-point step modes...............................................................................84
10.6.2 Weekday.................................................................................................................................85
10.6.3 Start time.................................................................................................................................85
10.6.4 Setpoint entry..........................................................................................................................86
10.6.5 Special controller functions via operation lines.......................................................................86
11. NOTIFICATION AND ALARM FUNCTIONS........................................................87
11.1 Notification and alarm messages overview.......................................................................................87
11.1.1 Notifications ............................................................................................................................87
11.1.2 Alarm messages.....................................................................................................................88
11.2 State of alarm....................................................................................................................................90
11.3 Resetting an alarm, list of active alarms...........................................................................................90
11.4 Activating / deactivating the audible alarm (alarm buzzer)...............................................................91
11.5 Tolerance range settings and alarm delay times..............................................................................91
11.6 Zero-voltage relay alarm output........................................................................................................93
12. TEMPERATURE SAFETY DEVICES...................................................................94
12.1 Over temperature protective device (class 1)...................................................................................94
12.2 Overtemperature safety controller class 3.1.....................................................................................94
12.2.1 Safety controller modes..........................................................................................................95
12.2.2 Setting the safety controller....................................................................................................95
12.2.3 Message and measures in the state of alarm.........................................................................96
12.2.4 Function check........................................................................................................................96
13. USER MANAGEMENT.........................................................................................97
13.1 Authorization levels and password protection...................................................................................97
13.2 Log in...............................................................................................................................................100
13.3 Log out ............................................................................................................................................101
13.4 User change....................................................................................................................................101
13.5 Password assignment and password change ................................................................................102
13.5.1 Password change .................................................................................................................102
13.5.2 Deleting the password for an individual authorization level..................................................104
13.5.3 New password assignment for “service” or “admin” authorization level when the password
function was deactivated ......................................................................................................105
13.6 Activation code................................................................................................................................106
14. GENERAL CONTROLLER SETTINGS..............................................................107
14.1 Selecting the controller’s menu language.......................................................................................107
14.2 Setting date and time......................................................................................................................107
14.3 Selecting the temperature unit........................................................................................................109
14.4 Display configuration.......................................................................................................................109
14.4.1 Adapting the display parameters..........................................................................................109
14.4.2 Touchscreen calibration........................................................................................................110
14.5 Network and communication...........................................................................................................111
14.5.1 Serial RS485 interface (available via BINDER INDIVIDUAL Customized Solutions) ..........111
14.5.2 Ethernet ................................................................................................................................112
14.5.2.1 Configuration.................................................................................................................112
14.5.2.2 Display of MAC address................................................................................................113
14.5.3 Web server............................................................................................................................113
14.5.4 E-Mail....................................................................................................................................114
14.6 USB menu: Data transfer via USB interface...................................................................................115

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15. GENERAL INFORMATION................................................................................116
15.1 Service contact page.......................................................................................................................116
15.2 Current operating parameters.........................................................................................................116
15.3 Event list..........................................................................................................................................117
15.4 Technical chamber information.......................................................................................................117
16. CHART RECORDER DISPLAY .........................................................................118
16.1 Views...............................................................................................................................................118
16.1.1 Show and hide legend..........................................................................................................118
16.1.2 Switch between legend pages..............................................................................................118
16.1.3 Show and hide specific indications.......................................................................................119
16.1.4 History display.......................................................................................................................119
16.2 Setting the parameters....................................................................................................................122
17. HUMIDIFICATION SYSTEM (CHAMBER WITH ACTIVE HUMIDIFICATION)..123
17.1 Function of the humidifying system.................................................................................................123
18. OPTIONS............................................................................................................124
18.1 Communication software APT-COM™ 3 DataControlSystem (option)...........................................124
18.2 Silicone access ports 30 mm / 1.18 in, closable from both sides with silicon plugs (option)..........124
18.3 Interior socket 230V (option, available via BINDER INDIVIDUAL Customized Solutions).............125
18.4 Analog outputs for temperature and CO2(option) ..........................................................................127
18.5 Data Logger kits (option).................................................................................................................127
18.6 Access port for extra-low voltage (option).......................................................................................128
18.7 BINDER Gas Supply Service – External bottle changer for CO2, N2or O2(option).......................129
18.8 Stands.............................................................................................................................................129
18.8.1 Stacking stand (option).........................................................................................................129
18.8.2 Stacking adapter for direct thermal decoupled stacking (option) .........................................129
18.8.3 Base on castors (option).......................................................................................................130
19. REFERENCE MEASUREMENTS ......................................................................130
19.1 CO2reference measuring ...............................................................................................................130
19.1.1 Measuring CO2concentration indirectly via the pH of the cell medium................................130
19.1.2 Measuring CO2directly via chemical indicator tubes ...........................................................131
19.1.3 Measuring CO2directly with an electronic infrared measuring device.................................131
19.2 Temperature reference measurement............................................................................................131
20. AVOIDING MICROBIAL CONTAMINATION......................................................132
20.1 Cells and media ..............................................................................................................................132
20.2 Laboratory conditions / equipment around the incubator................................................................132
20.3 Working and behavior in the lab .....................................................................................................132
20.4 Chamber design and equipment of the CO2incubator...................................................................133
20.5 Handling the CO2incubator ............................................................................................................134
21. CLEANING, DECONTAMINATION / DISINFECTION, AND STERILIZATION..135
21.1 Cleaning..........................................................................................................................................135
21.2 Decontamination / chemical disinfection of the chamber................................................................137
21.3 Hot-air sterilization at 180 °C / 356 °F.............................................................................................138
21.3.1 Overview...............................................................................................................................138
21.3.2 Preparation for a hot-air sterilization.....................................................................................139
21.3.3 Starting the sterilization cycle and running the hot-air sterilization.......................................140
21.3.4 Prematurely terminating the sterilization cycle.....................................................................141
21.3.4.1 Prematurely terminating the sterilization cycle after less than 4 hours: Ineffective
sterilization ....................................................................................................................142
21.3.4.2 Prematurely terminating the sterilization cycle after more than 4 hours, i.e., during the
cooling-down phase: successful sterilization................................................................142
21.3.5 Completing the entire sterilization cycle ...............................................................................143

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22. MAINTENANCE AND SERVICE........................................................................144
22.1 Maintenance intervals, service........................................................................................................144
22.2 Checking the humidity system fan..................................................................................................145
22.3 Gas inlet fine filter ...........................................................................................................................145
22.4 Replacing the CO2sensor...............................................................................................................145
22.5 Sending the chamber back to BINDER GmbH...............................................................................146
23. DISPOSAL .........................................................................................................147
23.1 Disposal of the transport packing....................................................................................................147
23.1.1 Outer chamber packing ........................................................................................................147
23.1.2 Packing inside the chamber and equipment.........................................................................147
23.2 Decommissioning............................................................................................................................148
23.3 Disposal of the chamber in the Federal Republic of Germany.......................................................148
23.4 Disposal of the chamber in the member states of the EU except for the Federal Republic of
Germany..........................................................................................................................................149
23.5 Disposal of the chamber in non-member states of the EU.............................................................150
24. TROUBLESHOOTING .......................................................................................151
24.1 General............................................................................................................................................151
24.2 Heating............................................................................................................................................151
24.3 Gas cylinder pressure too low.........................................................................................................152
24.4 Gas concentration...........................................................................................................................154
24.5 Sterilization......................................................................................................................................156
24.6 Humidity (chamber without active humidification)...........................................................................156
24.7 Humidity (chamber with active humidification)................................................................................156
24.8 Controller.........................................................................................................................................157
24.9 Open door.......................................................................................................................................157
25. TECHNICAL DESCRIPTION..............................................................................158
25.1 Factory calibration and adjustment.................................................................................................158
25.2 Over current protection ...................................................................................................................158
25.3 Definition of usable volume.............................................................................................................159
25.4 CB technical data............................................................................................................................159
25.5 Equipment and Options (extract)....................................................................................................161
25.6 Accessories and spare parts (extract) ............................................................................................163
25.7 Important conversion data for non-SI units.....................................................................................164
25.8 Conversion table for gas inlet pressures, bar – psi.........................................................................164
25.9 Dimensions .....................................................................................................................................165
26. CERTIFICATES AND DECLARATIONS OF CONFORMITY.............................166
26.1 EU Declaration of conformity ..........................................................................................................166
27. PRODUCT REGISTRATION..............................................................................168
28. CONTAMINATION CLEARANCE CERTIFICATE .............................................169
28.1 For chambers located outside the USA and Canada .....................................................................169
28.2 For chambers in the USA and Canada...........................................................................................172

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Dear customer,
For the correct operation of the CO2incubator CB, it is important that you read this operating manual
completely and carefully and observe all instructions as indicated. Failure to read, understand and follow
the instructions may result in personal injury. It can also lead to damage to the chamber and/or poor
equipment performance
1. Safety
This operating manual is part of the components of delivery. Always keep it handy for reference. The
device should only be operated by laboratory personnel especially trained for this purpose and familiar
with all precautionary measures required for working in a laboratory. Observe the national regulations on
minimum age of laboratory personnel. To avoid injuries and damage observe the safety instructions of the
operating manual.
WARNING
Failure to observe the safety instructions.
Serious injuries and chamber damage.
Observe the safety instructions in this operating manual.
Carefully read the complete operating instructions of the chamber.
1.1 Legal considerations
This operating manual is for informational purposes only. It contains information for installing, start-up,
operation and maintenance of the product. Note: the contents and the product described are subject to
change without notice.
Understanding and observing the instructions in this operating manual are prerequisites for hazard-free
use and safety during operation and maintenance. In no event shall BINDER be held liable for any
damages, direct or incidental arising out of or related to the use of this manual.
This operating manual cannot cover all conceivable applications. If you would like additional information,
or if special problems arise that are not sufficiently addressed in this manual, please ask your dealer or
contact us directly by phone at the number located on page one of this manual
Furthermore, we emphasize that the contents of this operating manual are not part of an earlier or
existing agreement, description, or legal relationship, nor do they modify such a relationship. All
obligations on the part of BINDER derive from the respective purchase contract, which also contains the
entire and exclusively valid statement of warranty administration. The statements in this manual neither
augment nor restrict the contractual warranty provisions.
1.2 Structure of the safety instructions
In this operating manual, the following safety definitions and symbols indicate dangerous situations
following the harmonization of ISO 3864-2 and ANSI Z535.6.
1.2.1 Signal word panel
Depending on the probability of serious consequences, potential dangers are identified with a signal
word, the corresponding safety color, and if appropriate, the safety alert symbol.
DANGER
Indicates an imminently hazardous situation that, if not avoided, will result in death or serious
(irreversible) injury.

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WARNING
Indicates a potentially hazardous situation which, if not avoided, could result in death or serious
(irreversible) injury.
CAUTION
Indicates a potentially hazardous situation which, if not avoided, may result in moderate or minor
(reversible) injury.
CAUTION
Indicates a potentially hazardous situation which, if not avoided, may result in damage to the product
and/or its functions or of a property in its proximity.
1.2.2 Safety alert symbol
Use of the safety alert symbol indicates a risk of injury.
Observe all measures that are marked with the safety alert symbol in order to avoid death or
injury.
1.2.3 Pictograms
Warning signs
Electrical hazard
Hot surface Explosive atmosphere Stability hazard
Lifting hazard
Gas cylinders
Suffocation hazard
CO2suffocation and
poisoning hazard
Explosive substances
Fire promoting agents
Harmful substances
Risk of corrosion and /
or chemical burns
Biohazard
Pollution Hazard
Magnetic field

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Mandatory action signs
Mandatory regulation Read operating
instructions
Disconnect the power
plug
Lift with several persons
Environment protection
Wear protective gloves
Wear safety goggles
Prohibition signs
Do NOT touch Do NOT spray with
water
Do NOT climb
Information to be observed in order to ensure optimum function of the product.
1.2.4 Word message panel structure
Type / cause of hazard.
Possible consequences.
Instruction how to avoid the hazard: prohibition
Instruction how to avoid the hazard: mandatory action
Observe all other notes and information not necessarily emphasized in the same way, in order to avoid
disruptions that could result in direct or indirect injury or property damage.

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1.3 Localization / position of safety labels on the chamber
The following labels are located on the chamber:
Pictograms (Warning signs)
Service label
Hot surface
•on the outer chamber door
Risk of injury
•on the outer door: CB-UL only
•above the access ports (option)
Magnetic field
•on the outer door above the door handle
CO2incubator CB CO2incubator CB-UL
Figure 1: Position of labels on the chamber
Keep safety labels complete and legible.
Replace safety labels that are no longer legible. Contact BINDER service for these replacements.