Binder CB 170 User manual

Issue 06/2018 Art. No. 7001-0326
Operating Manual
CB (E7)
CO2Incubators
CO2Incubators with O2Control
CO2Incubators with Active Humidification
with sterilizable NDIR sensor system for CO2
and microprocessor program controller MB2
Model Model version Voltage Equipment Art. no.
CB 170 CB170-230V 200-230 V 9040-0131, 9140-0131
CB 170 CB170-230V-O 200-230 V O2control 0-20 vol.-% 9040-0132, 9140-0132
CB 170 CB170-230V-F 200-230 V Active humidification 9040-0133, 9140-0133
CB 170 CB170-230V-
FO 200-230 V Active humidification
O2control 0-20 vol.-% 9040-0134, 9140-0134
CB 170-UL CB170-120V 100-120 V 9040-0139, 9140-0139
CB 170-UL CB170-120V-O 100-120 V O2control 0-20 vol.-% 9040-0140, 9140-0140
CB 170-UL CB170-120V-F 100-120 V Active humidification 9040-0141, 9140-0141
CB 170-UL CB170-120V-
FO 100-120 V Active humidification
O2control 0-20 vol.-% 9040-0142, 9140-0142
BINDER GmbH
Address: Post office box 102, 78502 Tuttlingen, Germany Phone: [phone]
Fax: [phone] Internet: http://www.binder-world.com
E-mail: [e-mail] Service Hotline: [phone]
Service Fax: [phone] Service E-Mail: [e-mail]
Service Hotline USA: [phone] or [phone] x3
Service Hotline Asia Pacific: [phone] or [phone]
Service Hotline Russia and CIS: [phone]

CB (E7) 06/2018 Page 2/174
Contents
1. SAFETY..................................................................................................................7
1.1 Legal considerations...........................................................................................................................7
1.2 Structure of the safety instructions......................................................................................................7
1.2.1 Signal word panel .....................................................................................................................7
1.2.2 Safety alert symbol...................................................................................................................8
1.2.3 Pictograms................................................................................................................................8
1.2.4 Word message panel structure.................................................................................................9
1.3 Localization / position of safety labels on the chamber ....................................................................10
1.4 Type plate .........................................................................................................................................11
1.5 General safety instructions on installing and operating the CO2incubator ......................................12
1.6 Precautions when working with gases..............................................................................................13
1.7 Precautions when handling gas cylinders.........................................................................................15
1.8 Intended use .....................................................................................................................................16
1.9 Measures to prevent accidents.........................................................................................................17
1.10 Resistance of the humidity sensor against harmful substances.......................................................18
2. CHAMBER DESCRIPTION..................................................................................19
2.1 Chamber overview............................................................................................................................20
2.2 Inner chamber...................................................................................................................................21
2.3 Control panel on the rear of the chamber.........................................................................................22
2.4 Instrument panel ...............................................................................................................................23
3. COMPLETENESS OF DELIVERY, TRANSPORTATION, STORAGE, AND
INSTALLATION....................................................................................................23
3.1 Unpacking, and checking equipment and completeness of delivery................................................23
3.2 Guidelines for safe lifting and transportation.....................................................................................24
3.3 Storage..............................................................................................................................................24
3.4 Location of installation and ambient conditions................................................................................25
4. INSTALLATION AND CONNECTIONS ...............................................................28
4.1 Shelves..............................................................................................................................................28
4.2 Permadry™ water pan......................................................................................................................28
4.3 Connecting the O2sensor (chamber with O2control).......................................................................29
4.4 Gas connections ...............................................................................................................................30
4.4.1 Connection of the CO2gas cylinder........................................................................................31
4.4.2 Connection of the O2gas cylinder (chamber with O2control with optional alternative control
range 10 up to 95 vol.-% O2) ..................................................................................................32
4.4.3 Connection of the N2gas cylinder (chamber with O2control).................................................33
4.4.4 Connecting the gas hose to the chamber rear (for CO2, O2, N2)............................................34
4.4.5 Gas cylinder connection kits (option)......................................................................................35
4.5 Water supply for the chamber with active humidification..................................................................36
4.5.1 Types of suitable water quality ...............................................................................................36
4.5.2 Water supply installation.........................................................................................................37
4.5.3 BINDER Pure Aqua Service (option)......................................................................................37
4.6 Electrical connection.........................................................................................................................38
4.7 Handling and aligning the divided inner door, gas proof (optional equipment).................................39
5. FUNCTIONAL OVERVIEW OF THE MB2 CHAMBER CONTROLLER...............40
5.1 Operating functions in normal display...............................................................................................41
5.2 Display views: Normal display, program display, chart-recorder display..........................................42
5.3 Controller icons overview..................................................................................................................43
5.4 Operating modes...............................................................................................................................45
5.5 Controller menu structure..................................................................................................................46
5.5.1 Main menu..............................................................................................................................47
5.5.2 “Settings” submenu.................................................................................................................48
5.5.3 “Service” submenu..................................................................................................................48

CB (E7) 06/2018 Page 3/174
5.6 Principle of controller entries.............................................................................................................49
5.7 Performance during and after power failures....................................................................................49
5.8 Performance when opening the door................................................................................................50
6. START UP............................................................................................................50
6.1 Turning on the chamber....................................................................................................................50
6.2 Controller settings upon start up.......................................................................................................51
6.3 Entering the altitude of the installation site .......................................................................................52
6.4 Factory settings.................................................................................................................................52
6.5 Equilibration time...............................................................................................................................53
6.6 Operating the DuoDoor™ door lock..................................................................................................53
6.7 Required gas supply for the chamber with O2control.......................................................................54
6.7.1 Hypoxic control range 0.2 vol.-% up to 20 vol.- % O2(regular)..............................................54
6.7.2 Alternative control range 10 vol.-% up to 95 vol.-% (option)..................................................54
6.7.3 Operation without O2control...................................................................................................54
6.8 Activating / deactivating CO2, O2and humidity control.....................................................................55
6.9 Humidity control of the Permadry™ system......................................................................................56
7. SET-POINT ENTRY IN “FIXED VALUE” OPERATING MODE...........................57
7.1 Set-point entry through the “Setpoints” menu...................................................................................58
7.2 Direct setpoint entry via Normal display............................................................................................59
7.3 Special controller functions via operation lines.................................................................................60
7.4 Safety instructions for setting high gas concentrations.....................................................................61
8. TIMER PROGRAM: STOPWATCH FUNCTION ..................................................62
8.1 Starting a timer program ...................................................................................................................62
8.1.1 Performance during program delay time ................................................................................62
8.2 Stopping a running timer program ....................................................................................................63
8.2.1 Pausing a running timer program ...........................................................................................63
8.2.2 Cancelling a running timer program........................................................................................63
8.3 Performance after the end of the program........................................................................................63
9. TIME PROGRAMS...............................................................................................64
9.1 Starting an existing time program.....................................................................................................64
9.1.1 Performance during program delay time ................................................................................65
9.2 Stopping a running time program......................................................................................................65
9.2.1 Pausing a running time program.............................................................................................65
9.2.2 Cancelling a running time program.........................................................................................65
9.3 Performance after the end of the program........................................................................................65
9.4 Creating a new time program............................................................................................................66
9.5 Program editor: program management.............................................................................................66
9.5.1 Deleting a time program .........................................................................................................67
9.6 Section editor: section management.................................................................................................68
9.6.1 Add a new program section....................................................................................................69
9.6.2 Copy and insert or replace a program section........................................................................69
9.6.3 Deleting a program section.....................................................................................................70
9.7 Value entry for a program section.....................................................................................................71
9.7.1 Section duration......................................................................................................................71
9.7.2 Set-point ramp and set-point step...........................................................................................72
9.7.3 Special controller functions via operation lines.......................................................................74
9.7.4 Setpoint entry..........................................................................................................................75
9.7.5 Tolerance range......................................................................................................................75
9.7.6 Repeating one or several sections within a time program......................................................76
9.7.7 Saving the time program.........................................................................................................77
10. WEEK PROGRAMS.............................................................................................78
10.1 Starting an existing week program....................................................................................................78
10.2 Cancelling a running week program .................................................................................................78
10.3 Creating a new week program..........................................................................................................79

CB (E7) 06/2018 Page 4/174
10.4 Program editor: program management.............................................................................................80
10.4.1 Deleting a week program........................................................................................................81
10.5 Section editor: section management.................................................................................................82
10.5.1 Add a new program section....................................................................................................83
10.5.2 Copy and insert or replace a program section........................................................................83
10.5.3 Deleting a program section.....................................................................................................84
10.6 Value entry for a program section.....................................................................................................84
10.6.1 Set-point ramp and set-point step modes...............................................................................84
10.6.2 Weekday.................................................................................................................................85
10.6.3 Start time.................................................................................................................................85
10.6.4 Setpoint entry..........................................................................................................................86
10.6.5 Special controller functions via operation lines.......................................................................86
11. NOTIFICATION AND ALARM FUNCTIONS........................................................87
11.1 Notification and alarm messages overview.......................................................................................87
11.1.1 Notifications ............................................................................................................................87
11.1.2 Alarm messages.....................................................................................................................88
11.2 State of alarm....................................................................................................................................90
11.3 Resetting an alarm, list of active alarms...........................................................................................90
11.4 Activating / deactivating the audible alarm (alarm buzzer)...............................................................91
11.5 Tolerance range settings and alarm delay times..............................................................................91
11.6 Zero-voltage relay alarm output........................................................................................................93
12. TEMPERATURE SAFETY DEVICES...................................................................94
12.1 Over temperature protective device (class 1)...................................................................................94
12.2 Overtemperature safety controller class 3.1.....................................................................................94
12.2.1 Safety controller modes..........................................................................................................95
12.2.2 Setting the safety controller....................................................................................................95
12.2.3 Message and measures in the state of alarm.........................................................................96
12.2.4 Function check........................................................................................................................96
13. USER MANAGEMENT.........................................................................................97
13.1 Authorization levels and password protection...................................................................................97
13.2 Log in...............................................................................................................................................100
13.3 Log out ............................................................................................................................................101
13.4 User change....................................................................................................................................101
13.5 Password assignment and password change ................................................................................102
13.5.1 Password change .................................................................................................................102
13.5.2 Deleting the password for an individual authorization level..................................................104
13.5.3 New password assignment for “service” or “admin” authorization level when the password
function was deactivated ......................................................................................................105
13.6 Activation code................................................................................................................................106
14. GENERAL CONTROLLER SETTINGS..............................................................107
14.1 Selecting the controller’s menu language.......................................................................................107
14.2 Setting date and time......................................................................................................................107
14.3 Selecting the temperature unit........................................................................................................109
14.4 Display configuration.......................................................................................................................109
14.4.1 Adapting the display parameters..........................................................................................109
14.4.2 Touchscreen calibration........................................................................................................110
14.5 Network and communication...........................................................................................................111
14.5.1 Serial RS485 interface (available via BINDER INDIVIDUAL Customized Solutions) ..........111
14.5.2 Ethernet ................................................................................................................................112
14.5.2.1 Configuration.................................................................................................................112
14.5.2.2 Display of MAC address................................................................................................113
14.5.3 Web server............................................................................................................................113
14.5.4 E-Mail....................................................................................................................................114
14.6 USB menu: Data transfer via USB interface...................................................................................115

CB (E7) 06/2018 Page 5/174
15. GENERAL INFORMATION................................................................................116
15.1 Service contact page.......................................................................................................................116
15.2 Current operating parameters.........................................................................................................116
15.3 Event list..........................................................................................................................................117
15.4 Technical chamber information.......................................................................................................117
16. CHART RECORDER DISPLAY .........................................................................118
16.1 Views...............................................................................................................................................118
16.1.1 Show and hide legend..........................................................................................................118
16.1.2 Switch between legend pages..............................................................................................118
16.1.3 Show and hide specific indications.......................................................................................119
16.1.4 History display.......................................................................................................................119
16.2 Setting the parameters....................................................................................................................122
17. HUMIDIFICATION SYSTEM (CHAMBER WITH ACTIVE HUMIDIFICATION)..123
17.1 Function of the humidifying system.................................................................................................123
18. OPTIONS............................................................................................................124
18.1 Communication software APT-COM™ 3 DataControlSystem (option)...........................................124
18.2 Silicone access ports 30 mm / 1.18 in, closable from both sides with silicon plugs (option)..........124
18.3 Interior socket 230V (option, available via BINDER INDIVIDUAL Customized Solutions).............125
18.4 Analog outputs for temperature and CO2(option) ..........................................................................127
18.5 Data Logger kits (option).................................................................................................................127
18.6 Access port for extra-low voltage (option).......................................................................................128
18.7 BINDER Gas Supply Service – External bottle changer for CO2, N2or O2(option).......................129
18.8 Stands.............................................................................................................................................129
18.8.1 Stacking stand (option).........................................................................................................129
18.8.2 Stacking adapter for direct thermal decoupled stacking (option) .........................................129
18.8.3 Base on castors (option).......................................................................................................130
19. REFERENCE MEASUREMENTS ......................................................................130
19.1 CO2reference measuring ...............................................................................................................130
19.1.1 Measuring CO2concentration indirectly via the pH of the cell medium................................130
19.1.2 Measuring CO2directly via chemical indicator tubes ...........................................................131
19.1.3 Measuring CO2directly with an electronic infrared measuring device.................................131
19.2 Temperature reference measurement............................................................................................131
20. AVOIDING MICROBIAL CONTAMINATION......................................................132
20.1 Cells and media ..............................................................................................................................132
20.2 Laboratory conditions / equipment around the incubator................................................................132
20.3 Working and behavior in the lab .....................................................................................................132
20.4 Chamber design and equipment of the CO2incubator...................................................................133
20.5 Handling the CO2incubator ............................................................................................................134
21. CLEANING, DECONTAMINATION / DISINFECTION, AND STERILIZATION..135
21.1 Cleaning..........................................................................................................................................135
21.2 Decontamination / chemical disinfection of the chamber................................................................137
21.3 Hot-air sterilization at 180 °C / 356 °F.............................................................................................138
21.3.1 Overview...............................................................................................................................138
21.3.2 Preparation for a hot-air sterilization.....................................................................................139
21.3.3 Starting the sterilization cycle and running the hot-air sterilization.......................................140
21.3.4 Prematurely terminating the sterilization cycle.....................................................................141
21.3.4.1 Prematurely terminating the sterilization cycle after less than 4 hours: Ineffective
sterilization ....................................................................................................................142
21.3.4.2 Prematurely terminating the sterilization cycle after more than 4 hours, i.e., during the
cooling-down phase: successful sterilization................................................................142
21.3.5 Completing the entire sterilization cycle ...............................................................................143

CB (E7) 06/2018 Page 6/174
22. MAINTENANCE AND SERVICE........................................................................144
22.1 Maintenance intervals, service........................................................................................................144
22.2 Checking the humidity system fan..................................................................................................145
22.3 Gas inlet fine filter ...........................................................................................................................145
22.4 Replacing the CO2sensor...............................................................................................................145
22.5 Sending the chamber back to BINDER GmbH...............................................................................146
23. DISPOSAL .........................................................................................................147
23.1 Disposal of the transport packing....................................................................................................147
23.1.1 Outer chamber packing ........................................................................................................147
23.1.2 Packing inside the chamber and equipment.........................................................................147
23.2 Decommissioning............................................................................................................................148
23.3 Disposal of the chamber in the Federal Republic of Germany.......................................................148
23.4 Disposal of the chamber in the member states of the EU except for the Federal Republic of
Germany..........................................................................................................................................149
23.5 Disposal of the chamber in non-member states of the EU.............................................................150
24. TROUBLESHOOTING .......................................................................................151
24.1 General............................................................................................................................................151
24.2 Heating............................................................................................................................................151
24.3 Gas cylinder pressure too low.........................................................................................................152
24.4 Gas concentration...........................................................................................................................154
24.5 Sterilization......................................................................................................................................156
24.6 Humidity (chamber without active humidification)...........................................................................156
24.7 Humidity (chamber with active humidification)................................................................................156
24.8 Controller.........................................................................................................................................157
24.9 Open door.......................................................................................................................................157
25. TECHNICAL DESCRIPTION..............................................................................158
25.1 Factory calibration and adjustment.................................................................................................158
25.2 Over current protection ...................................................................................................................158
25.3 Definition of usable volume.............................................................................................................159
25.4 CB technical data............................................................................................................................159
25.5 Equipment and Options (extract)....................................................................................................161
25.6 Accessories and spare parts (extract) ............................................................................................163
25.7 Important conversion data for non-SI units.....................................................................................164
25.8 Conversion table for gas inlet pressures, bar – psi.........................................................................164
25.9 Dimensions .....................................................................................................................................165
26. CERTIFICATES AND DECLARATIONS OF CONFORMITY.............................166
26.1 EU Declaration of conformity ..........................................................................................................166
27. PRODUCT REGISTRATION..............................................................................168
28. CONTAMINATION CLEARANCE CERTIFICATE .............................................169
28.1 For chambers located outside the USA and Canada .....................................................................169
28.2 For chambers in the USA and Canada...........................................................................................172

CB (E7) 06/2018 Page 7/174
Dear customer,
For the correct operation of the CO2incubator CB, it is important that you read this operating manual
completely and carefully and observe all instructions as indicated. Failure to read, understand and follow
the instructions may result in personal injury. It can also lead to damage to the chamber and/or poor
equipment performance
1. Safety
This operating manual is part of the components of delivery. Always keep it handy for reference. The
device should only be operated by laboratory personnel especially trained for this purpose and familiar
with all precautionary measures required for working in a laboratory. Observe the national regulations on
minimum age of laboratory personnel. To avoid injuries and damage observe the safety instructions of the
operating manual.
WARNING
Failure to observe the safety instructions.
Serious injuries and chamber damage.
Observe the safety instructions in this operating manual.
Carefully read the complete operating instructions of the chamber.
1.1 Legal considerations
This operating manual is for informational purposes only. It contains information for installing, start-up,
operation and maintenance of the product. Note: the contents and the product described are subject to
change without notice.
Understanding and observing the instructions in this operating manual are prerequisites for hazard-free
use and safety during operation and maintenance. In no event shall BINDER be held liable for any
damages, direct or incidental arising out of or related to the use of this manual.
This operating manual cannot cover all conceivable applications. If you would like additional information,
or if special problems arise that are not sufficiently addressed in this manual, please ask your dealer or
contact us directly by phone at the number located on page one of this manual
Furthermore, we emphasize that the contents of this operating manual are not part of an earlier or
existing agreement, description, or legal relationship, nor do they modify such a relationship. All
obligations on the part of BINDER derive from the respective purchase contract, which also contains the
entire and exclusively valid statement of warranty administration. The statements in this manual neither
augment nor restrict the contractual warranty provisions.
1.2 Structure of the safety instructions
In this operating manual, the following safety definitions and symbols indicate dangerous situations
following the harmonization of ISO 3864-2 and ANSI Z535.6.
1.2.1 Signal word panel
Depending on the probability of serious consequences, potential dangers are identified with a signal
word, the corresponding safety color, and if appropriate, the safety alert symbol.
DANGER
Indicates an imminently hazardous situation that, if not avoided, will result in death or serious
(irreversible) injury.

CB (E7) 06/2018 Page 8/174
WARNING
Indicates a potentially hazardous situation which, if not avoided, could result in death or serious
(irreversible) injury.
CAUTION
Indicates a potentially hazardous situation which, if not avoided, may result in moderate or minor
(reversible) injury.
CAUTION
Indicates a potentially hazardous situation which, if not avoided, may result in damage to the product
and/or its functions or of a property in its proximity.
1.2.2 Safety alert symbol
Use of the safety alert symbol indicates a risk of injury.
Observe all measures that are marked with the safety alert symbol in order to avoid death or
injury.
1.2.3 Pictograms
Warning signs
Electrical hazard
Hot surface Explosive atmosphere Stability hazard
Lifting hazard
Gas cylinders
Suffocation hazard
CO2suffocation and
poisoning hazard
Explosive substances
Fire promoting agents
Harmful substances
Risk of corrosion and /
or chemical burns
Biohazard
Pollution Hazard
Magnetic field

CB (E7) 06/2018 Page 9/174
Mandatory action signs
Mandatory regulation Read operating
instructions
Disconnect the power
plug
Lift with several persons
Environment protection
Wear protective gloves
Wear safety goggles
Prohibition signs
Do NOT touch Do NOT spray with
water
Do NOT climb
Information to be observed in order to ensure optimum function of the product.
1.2.4 Word message panel structure
Type / cause of hazard.
Possible consequences.
Instruction how to avoid the hazard: prohibition
Instruction how to avoid the hazard: mandatory action
Observe all other notes and information not necessarily emphasized in the same way, in order to avoid
disruptions that could result in direct or indirect injury or property damage.

CB (E7) 06/2018 Page 10/174
1.3 Localization / position of safety labels on the chamber
The following labels are located on the chamber:
Pictograms (Warning signs)
Service label
Hot surface
•on the outer chamber door
Risk of injury
•on the outer door: CB-UL only
•above the access ports (option)
Magnetic field
•on the outer door above the door handle
CO2incubator CB CO2incubator CB-UL
Figure 1: Position of labels on the chamber
Keep safety labels complete and legible.
Replace safety labels that are no longer legible. Contact BINDER service for these replacements.

CB (E7) 06/2018 Page 11/174
1.4 Type plate
Position of type plate: left chamber side (seen from front), at the bottom in the middle
Figure 2: Type plate (example of CB 170 regular chamber)
Indications of the type plate (example)
Information
BINDER
Manufacturer: BINDER GmbH
CB 170
Model designation
CO2 incubator
Device name
Serial No.
00000000000000
Serial no. of the chamber
Built
2018
Year of construction
Nominal
temperature
187 °C
369 °F
Nominal temperature
IP protection
20
IP type of protection acc. to standard EN 60529
Temp. safety device
DIN 12880
Temperature safety device acc. to standard DIN 12880
Class
3.1
Class of temperature safety device
Art. No.
9040-0131
Art. No. of the chamber
Project No.
---
Optional: Special application acc. to project no.
1,30 kW
Nominal power
200-230 V / 50 Hz
Nominal voltage range +/-10%
at the indicated power frequency
200-230 V / 60 Hz
1 N ~
Current type
5,7 A
Nominal current
Symbol on the type plate
Information
CE conformity marking
Electrical and electronic equipment manufactured / placed
on the market in the EC after 13 August 2005 and to be
disposed of in separate collection according to Directive
2012/19/EU on waste electrical and electronic equipment
(WEEE).
Nominal temp.
187 °C
1,30 kW / 5,7 A
369 °F
200-230 V / 50 Hz
IP protection
20
200-230 V / 60 Hz
Safety device
DIN 12880
1 N ~
Class
3.1
Art. No.
9040-0131
Project No.
Built
2018
CO2 incubator
BINDER GmbH
Im Mittleren Ösch 5
78532 Tuttlingen / Germany
www.binder-world.com
CB 170
E7
Serial No. 00000000000000
Made in Germany

CB (E7) 06/2018 Page 12/174
1.5 General safety instructions on installing and operating the CO2incubator
With regard to operating the chamber and to the installation location, please observe the guideline
BGI/GUV-I 850-0 on safe working in laboratories (formerly BGR/GUV-R 120 or ZH 1/119 laboratory
guidelines issued by the employers’ liability insurance association) (for Germany).
BINDER GmbH is only responsible for the safety features of the chamber provided skilled electricians or
qualified personnel authorized by BINDER perform all maintenance and repair, and if components
relating to chamber safety are replaced in the event of failure with original spare parts.
To operate the chamber, use only original BINDER accessories or accessories from third-party suppliers
authorized by BINDER. The user is responsible for any risk caused by using unauthorized accessories.
CAUTION
Danger of overheating.
Damage to the chamber.
∅Do NOT install the chamber in unventilated recesses.
Ensure sufficient ventilation for dispersal of the heat.
Do not operate the chamber in hazardous locations.
DANGER
Explosion hazard.
Danger of death.
∅Do NOT operate the chamber in potentially explosive areas.
KEEP explosive dust or air-solvent mixtures AWAY from the chamber.
The chamber does not dispose of any measures of explosion protection.
DANGER
Explosion hazard.
Danger of death.
∅Do NOT introduce any substance into the chamber which is combustible or explosive at
working temperature.
∅NO explosive dust or air-solvent mixture in the inner chamber.
Any solvent contained in the charging material must not be explosive or inflammable. I.e., irrespective of
the solvent concentration in the steam room, NO explosive mixture with air must form. The temperature
inside the chamber must lie below the flash point or below the sublimation point of the charging material.
Familiarize yourself with the physical and chemical properties of the charging material, as well as the
contained moisture constituent and its behavior with the addition of heat energy and humidity (chamber
with active humidification).
Familiarize yourself with any potential health risks caused by the charging material, the contained
moisture constituent or by reaction products which may arise during the temperature process. Take
adequate measures to exclude such risks prior to putting the chamber into operation.
DANGER
Electrical hazard.
Danger of death.
∅The chamber must NOT become wet during operation or maintenance.
The chambers were produced in accordance with VDE regulations and were routinely tested in
accordance to VDE 0411-1 (IEC 61010-1).

CB (E7) 06/2018 Page 13/174
During and after a sterilization the temperature of the inner surfaces almost equals the set-point.
CAUTION
The inner doors, the inner door and glass door handles, and the inner chamber will
become hot during a sterilization.
Danger of burning.
∅Do NOT touch the inner doors, the inner door and glass door handles, and the inner
surfaces after a sterilization.
WARNING
Stability hazard.
Danger of injury.
Damage to the chamber and the charging material.
Housing cover breakaway.
∅Do NOT climb on the lower housing cover.
∅Do NOT load the lower housing cover with heavy objects while the chamber door is
open.
1.6 Precautions when working with gases
Notes on handling carbon dioxide (CO2)
Carbon dioxide (CO2) in high concentrations is hazardous to health. It is colorless and almost odorless
and therefore practically imperceptible. Vent out any CO2gas that may escape via good room ventilation
or a suitable connection to an exhaust system. We recommend installing a CO2warning system
WARNING
High concentration of CO2(> 4 Vol.-%).
Risk of death by suffocation.
Danger of poisoning.
∅Do NOT set up chambers in non-ventilated recesses.
Ensure technical ventilation measures.
Observe the relevant regulations for handling CO2.
Chamber with O2control: Notes on handling oxygen (O2)
Oxygen (O2) is colorless and almost odorless and therefore practically imperceptible. It promotes burns,
which can proceed explosively. There is a fire hazard for flammable oxygenated materials, e.g. clothes
and hair. O2is heavier than air and may accumulate in low-lying areas.
WARNING
High concentration of O2(> 21 % O2).
Fire and explosion hazard through contact of combustible materials with O2.
Risk of burns and other injuries.
∅Do NOT set up chambers in non-ventilated recesses.
Ensure technical ventilation measures.
Observe the relevant regulations for handling O2.

CB (E7) 06/2018 Page 14/174
Take appropriate measures to prevent oxygen enrichment and fire and explosion hazards in areas where
oxygen enrichment is possible.
General information for safe handling of oxygen:
•Make sure training of personnel on hazards of oxygen enrichmen
t and necessary safety
measures.
•Make sure adequate labeling of all oxygen equipment and facilities.
•
Make sure gas tightness of all gas connections by checking them for leaks (e.g. with leak
spray or diluted soap solution).
•Close the main valve of the source of oxygen after work when not using the chamber.
•Never lubricate O2
equipment with oil or fat. Use only materials and spare parts which are
approved for use with oxygen.
•Regularly inspect fire extinguishers for proper condition.
•Set up emergency showers where oxygen enrichment is possible.
•
Strictest smoking ban and no ignition sources in areas where oxygen enrichment is
possible.
•
Make sure good ventilation of areas where oxygen enrichment is possible (location of the
chamber and/or O2cylinders.
•Persons who may have been in a possibly oxygen-
enriched atmosphere must keep away
from ignition sources (flames, cigarettes, etc.) and ventilate their clothes at least 15
minutes.
•Always keep emergency routes free.
Chamber with O2control: Notes on handling nitrogen (N2)
Nitrogen (N2) in high concentrations is hazardous to health. It is colorless and almost odorless and
therefore practically imperceptible. Any N2gas that may escape must be safely led out via good room
ventilation or a suitable connection to an exhaust system.
WARNING
High concentration of N2.
Risk of death by suffocation.
∅Do NOT set up chambers in non-ventilated recesses.
Ensure technical ventilation measures.
Observe the relevant regulations for handling N2.

CB (E7) 06/2018 Page 15/174
1.7 Precautions when handling gas cylinders
General information for safe handling of gas cylinders:
•Store and use gas cylinders only in well-ventilated locations.
•Open the gas cylinder valve slowly to avoid pressure surges.
•Secure gas cylinders during storage and use against falling (chaining).
•Transport gas cylinders with a cylinder cart, do not carry, roll, or throw them.
•
Always close the valve even with apparently empty cylinders; screw on the cap when not in
use. Return gas cylinders with the valve closed.
•Do not open gas cylinders by force. Mark them when damaged.
•Protect gas cylinders against fire, e.g. do not store together with flammable liquids.
•Observe relevant regulations for dealing with gas cylinders.
Secure the gas cylinders against falling and other mechanical damage.
WARNING
Safety valve tearing off.
Sudden release of the stored pressure energy.
Risk of injury.
Secure gas cylinders against falling (chaining).
Transport gas cylinders with a cylinder cart.
The valve of the gas cylinder always must be closed before screwing on or unscrewing the gas hose.
WARNING
Opening the cylinder valve when the cylinder is not connected.
Sudden release of the stored pressure energy.
Risk of injury.
Close the gas cylinder valve before connecting or removing the gas hose.
After connecting the gas cylinder, check all gas connections for leaks (e.g. with leak spray or
diluted soap solution).

CB (E7) 06/2018 Page 16/174
1.8 Intended use
Series CB incubators are suitable for the cultivation of mammal cells under typical conditions of approx.
37 °C / 98.6°F. The chamber permits setting defined pH conditions by common NaHCO3buffer systems
of commercial cell media by keeping an exact CO2atmosphere inside. The chambers guarantee high
humidity inside to avoid osmolarity increasing caused by the evaporation of the cell media.
With the chamber with O2control, a variable oxygen atmosphere can additionally influence the growth of
the cells.
The chambers are suitable for exact conditioning of harmless materials. Any possible solvent any solvent
must not be explosive and flammable. Components of the charging material must NOT form an explosive
mixture with air. The operating temperature must lie below the flash point or below the sublimation point
of the charging material. Any component of the charging material must NOT be able to release toxic
gases.
DANGER
Explosion or implosion hazard.
Danger of poisoning.
Danger of death.
∅Do NOT
introduce any substance combustible or explosive at working temperature into
the chamber, in particular no energy sources such as batteries or lithium-ion batteries.
∅NO explosive dust or air-solvent mixture in the inner chamber.
∅Do NOT introduce any substance which could lead to release of toxic gases.
Other applications are not approved.
Following the instructions in this operating manual and conducting regular maintenance work
(chap. 22.1) are part of the intended use.
The chambers are not classified as medical devices as defined by the Medical Device Directive
93/42/EEC.
Due to the special demands of the Medical Device Directive (MDD), these chambers are not
qualified for sterilization of medical devices as defined by the directive 93/42/EWG.
WARNING: If customer should use a BINDER chamber running in non-supervised
continuous operation, we strongly recommend in case of inclusion of irrecoverable specimen
or samples to split such specimen or samples and store them in at least two chambers, if this
is feasible.
The charging material shall not contain any corrosive ingredients that may damage the
machine components made of stainless steel, aluminum, and copper. Such ingredients
include in particular acids and halides. Any corrosive damage caused by such ingredients is
excluded from liability by BINDER GmbH.
In case of foreseeable use of the chamber there is no risk for the user through the integration of the
chamber into systems or by special environmental or operating conditions in the sense of EN 61010-
1:2010. For this, the intended use of the chamber and all its connections must be observed.

CB (E7) 06/2018 Page 17/174
1.9 Measures to prevent accidents
The manufacturer took the following measures to prevent dangers:
•Indications on the type plate
See operating manual chap. 1.4.
•Operating manual
An operating manual is available for each chamber.
•Overtemperature monitoring
The chamber is equipped with a temperature display, which can be read from outside.
The chamber is equipped with an additional safety controller (temperature safety device class 3.1 acc.
to DIN 12880:2007). Visual and audible (buzzer) signals indicate temperature exceeding.
•Safety, measurement, and control equipment
The safety, measuring, and control equipment is easily accessible.
•Electrostatic charge
The interior parts are grounded.
•Non-ionizing radiation
Non-ionizing radiation is not intentionally produced, but released only for technical reasons by
electrical equipment (e.g. power cables). The machine is equipped with a permanent magnet located
behind the inner panel of the outer chamber door. If persons with active implants (e.g. pacemakers,
defibrillators) keep a safe distance (distance of field source to implant) of 30 cm, an influence of these
implants can be excluded with high probability.
•Protection against touchable surfaces
Tested according to EN ISO 13732-1:2008.
•Floors
See operating manual chap. 3.4 for correct installation
•Cleaning
See operating manual chap. 21.

CB (E7) 06/2018 Page 18/174
1.10 Resistance of the humidity sensor against harmful substances
The following list of harmful substances refers only to the humidity sensor and does not include any other
materials incorporated in the chamber or prohibited substances in relation to explosion protection.
Some gases - especially clean gases - do not have any influence on the humidity sensor. Others do have
a very small influence, whereas others may influence the sensor to a larger extent.
•The following gases do not influence the sensor and the humidity measurement: Argon (Ar), carbon
dioxide (CO2),helium (He), hydrogen (H2), neon (Ne), nitrogen (N2), nitrous oxide (N2O), oxygen (O2)
•The following gases do not or to a minor extent influence the sensor and the humidity measurement:
Butane (C4H10), ethane (C2H6), methane (CH4), natural gas propane (C3H8)
•The following gases do not, or to a minor extent influence the sensor and the humidity measurement,
provided that the indicated loads are not exceeded:
Maximum work place
threshold limit value
Tolerated concentration
with permanent load
Substance
Formula
ppm
mg/m3
ppm
mg/m3
Ammonia
NH3
20
14
5500
4000
Acetone
CH3COCH3
500
1200
3300
8000
Benzene
300
1200
150000
Chlorine
Cl2
0.5
1.5
0.7
2
Acetic acid
CH3COOH
10
25
800
2000
Ethyl acetate
CH3COOC2H5
400
1400
4000
15000
Ethanol
C2H5OH
500
960
3500
6000
Ethylene glycol
HOCH2CH2OH
10
26
1200
3000
Formaldehyde
HCHO
0.3
0.37
2400
3000
Isopropanol
(CH3)2CHOH
200
500
4800
12000
Methanol
CH3OH
200
260
3500
6000
Methyl ethyl ketone
C2H5COCH3
200
590
3300
8000
Ozone
O3
0.1
0.2
0.5
1
Hydrochloric acid
HCl
2
3
300
500
Hydrogen sulphide
H2S
10
15
350
500
Nitrogen oxides
NOx
5
9
5
9
Sulphur dioxide
SO2
5
13
5
13
Toluol
C6H5CH3
100
380
1300
5000
Xylene
C6H4(CH3)2
100
440
1300
5000
These values are to be considered as approximate values. The sensor resistance largely depends on
the temperature and humidity conditions during the time of exposure to harmful substances. Avoid
simultaneous condensation. Tolerated error of measurement: ± 2 %r.H. The maximum work place
threshold limit value is one that can be regarded as harmless for humans.
•Vapors of oil and fat are dangerous for the sensor because they may condensate at the sensor and
thus prevent its function (insulating layer). For similar reasons it is not possible to measure smoke
gases.

CB (E7) 06/2018 Page 19/174
2. Chamber description
The CO2incubators CB are equipped with a multifunctional microprocessor display controller for
temperature, CO2, and O2(chamber with O2control) levels and humidity (chamber with active
humidification) and a digital display accurate to one-tenth of a degree resp. 0.1 vol.-%. With its
comprehensive program control functions, the display program controller MB2 permits the high precision
performance of temperature, CO2, O2(chamber with O2control), and humidity (chamber with active
humidification) cycles.
Material: The inner chamber, the pre-heating chamber and the inside of the doors are all made of
stainless steel V2A (German material no. 1.4301, US equivalent AISI 304). The inner surfaces are
smooth and therefore easy to clean. The inner chamber is deep-drawn from one piece, polished (suitable
for pharmaceutical applications) and has no welds or inaccessible corners. The hinges and the seal of the
inner glass door are glued from the outside to aid cleaning of the inner chamber. When operating the
chamber at high temperatures (sterilization), the impact of the oxygen in the air may cause discoloration
of the metallic surfaces (yellowish-brown or blue) by natural oxidation processes. These colorations are
harmless and will in no way impair the function or quality of the chamber. The perforated shelves are also
made of stainless steel. You can insert a maximum of 3 (CB 56), 6 (CB 170), resp. 8 (CB 220) shelves.
The housing is RAL 7035 powder-coated. All corners and edges are also completely coated.
Door lock: The DuoDoor™ door lock offers two to open the outer door and glass door independently or
together. The outer door is regularly equipped with a door lock with keys.
Sterilization: The chamber’s heating system permits hot-air auto-sterilization at a setpoint of 187.5 °C /
369.5°F. Thus, a temperature of 180 °C / 356°F is maintained for at least 30 minutes on all internal
surfaces, resulting in sterilization of the entire inner chamber. Therefore, this procedure meets all
international guidelines regarding hot air sterilization, e.g. AAMI ST63, DIN 58947, European
Pharmacopoeia.
Temperature safety device Thanks to the regular safety controller (temperature safety device class 3.1
acc. to DIN 12880:2007), the set temperature is maintained in case of failure.
CO2system: A highly precise, drift-free CO2infrared measuring system in combination with the
permanent mixture of CO2gas through a special proprietary gas mixing head developed by BINDER
allows precise and constant CO2concentrations for long periods. This creates optimum growth conditions
for cultures. The gas enters the chamber via a fine filter (aseptic filter) with a high filtration efficiency that
also filters the smallest particles.
Fast reaction times, maximum accuracy and selectivity characterize the CO2measuring procedure of the
CB incubator series. The accuracy of the CO2 measuring system is based on an infrared measuring cell
with NDIR (non-dispersive infrared) sensor, which continuously regulates to a reference value. Therefore,
disturbance variables and aging phenomena in the measuring system are almost completely eliminated,
so that this measuring system, in contrast to other measuring procedures, remains practically drift-free
between calibrations and is entirely selective for CO2. The sensor is built into the chamber and can be
sterilized.
The accuracy of the indicated values of CO2depends on the ambient air pressure (approx. 0.08 vol.-%
per 10 mbar / 0.15 psi). To compensate for this effect in the CO2measurement, the altitude of the
installation site is entered into the controller.
O2control (chamber with O2control): The CO2incubator is available with O2control in addition to CO2
control. There are two different control ranges:
•Regular equipment: Hypoxic control range 0.2 to 20 vol. % O2. Only N2can be connected to reduce
O2concentration; it is not possible to connect O2gas bottles to increase O2concentration. Control in
the low O2range is very precise, in particular in the range below 1 vol. % O2.
•Alternative control range 10 to 95 vol. % O2(option). Although the high control range is intended in
particular for hyperoxic applications (> 21 vol. % O2), it is also suitable for slightly hypoxic applications
between 10 and 20 vol. % O2.
The O2sensor is a semiconductor gas sensor with ZrO2ceramic.

CB (E7) 06/2018 Page 20/174
Humidity control (chamber with active humidification):The chamber is available with humidity control
and a microprocessor-controlled humidification system.
Freshwater is supplied by manually filling a freshwater bag, which is placed behind the chamber door in a
recess.
A resistance humidifying system humidifies the air. For this purpose, use deionized (demineralized)
water. The option BINDER Pure Aqua Service allows using the chamber with any degree of water
hardness.
Controller: The efficient program controller is equipped with a multitude of operating functions, in
addition to recorder and alarm functions. Programming of test cycles is easily accomplished via the
modern MB2 touch screen controller and is also possible directly with a computer via Intranet in
connection with the BINDER communication software APT-COM™ 3 DataControlSystem (option, chap.
18.1). The chambers are equipped with an Ethernet interface for computer communication. The APT-
COM™ 3 software permits networking up to 30 chambers and connecting them to a PC for controlling
and programming, as well as recording and representing temperature, CO2and O2and humidity data. For
further options, see chap. 25.5.
Temperature range: 4 °C / 7.2 °F above ambient temperature up to +60 °C / 140 °F
Temperature range
(chamber with O2control): 6 °C / 10.8 °F above ambient temperature up to +60 °C / 140 °F
CO2range: 0 vol.-% up to 20 vol.-%
O2range
(chamber with O2control): 0.2 vol.-% up to 20 vol.-% (hypoxic control range)
or 10 vol.-% up to 95 vol.-% (optional alternative control range)
Humidity range
(chamber with active humidification): 60 % r.h. up to 93 % r.h.
2.1 Chamber overview
Instrument panel with microprocessor controller
T4.12 and USB interface
Door handle
Chamber door
Main power switch
Figure 3: CO2incubator CB (example: model CB 170)