Diesse CHORUS AUTOSAMPLER User manual

Installation guide
Autosampler software version 0.20 –October 03rd 2017
Chorus Trio CPU 2010 4.0.6.39 software version –September 07th 2017
Revision 0 October 26th 2017

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SUMMARY
1. TECHNICAL SPECIFICATIONS...................................................................................................................... 3
2. STORAGE AND TRANSPORTATION ............................................................................................................ 4
3. PREPARATION AND CHECKS BEFORE INSTALLATION ................................................................................ 5
4. INSTALLATION REQUIREMENTS AND MATERIALS..................................................................................... 6
5. UNPACKING ............................................................................................................................................... 7
6. PLACEMENT ............................................................................................................................................... 9
7. INSTALLATION.......................................................................................................................................... 11
8. FUNCTIONAL STARTING PROCEDURE...................................................................................................... 19
8.1 SETTING DISPENSING POSITION ...................................................................................................... 21
8.2 TIP PICKING ADJUSTMENT............................................................................................................... 22
8.3 SAMPLE COLLECTION SETTING........................................................................................................ 24
8.4 BARCODE READER POSITION SETTING ............................................................................................ 25
8.5 ROTOR PARAMETERS....................................................................................................................... 27
8.6 X AXIS PARAMETERS........................................................................................................................ 28
8.7 DILUTER PARAMETERS .................................................................................................................... 29
8.8 Z AXIS PARAMETERS ........................................................................................................................ 30
8.9 TIP GRABBING PARAMETERS........................................................................................................... 31
8.10 LEVEL RESEARCH SETTINGS ............................................................................................................. 33
8.10.1 Pressure control procedure..................................................................................................... 35
8.11 SUCTION VALUES CALIBRATION ...................................................................................................... 35
8.12 SAMPLE TEST TUBE PARAMETERS................................................................................................... 36
8.13 CALIBRATOR TEST TUBE PARAMETERS............................................................................................ 37
9. DEFAULT VALUES..................................................................................................................................... 38
10. FIRMWARE UPDATE............................................................................................................................. 39
11. TOUCH SCREEN CALIBRATION ............................................................................................................. 40
12. SCREENSHOT CAPTURING ................................................................................................................... 41
AUTOSAMPLER CHORUS MODELS
This guide applies to the following Chorus Autosampler models:
MODEL
CODE
81210
Chorus Autosampler

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1. TECHNICAL SPECIFICATIONS
Instrument size
Length: 365mm
Height: 581mm
Depth: 288mm
Weight: 10Kg
The dimensions refer to the closed
instrument. Weight is distributed over 4 legs.
Packaging size
Length: 500mm
Height: 581mm
Depth: 288mm
Weight: 2Kg
Features
Installation category: II
Pollution degree: 2
For internal use
Noise level < 80 dB
Insertion devices section
30 positions for tubes
32 positions for disposable tips
Transfer device
Micro Diluter Stepper Motor 500µl-5000µl
Screen
4,3" WVGA 16.7M Colour RGB Vertical Strips
Size:
105.5(W) mm x 67.2(H) mm x 4.05(D) mm
Resolution:
480 x 3(RGB)(W) x 272(H) dot
Internal barcode
Leuze Electronic: Scan Engine CCD model
CR50
Environmental requirements:
(Operating) ambient temperature: from + 15 °C to + 35
°C
(Storage) ambient temperature: from + 5 °C to + 45 °C
Relative humidity: 20% to 80% without condensation
Altitude: 0 to 2,000 Mt
Ambient lighting
No explosive gas diffused
No dust diffused
Air change not required
Electrical requirements
Power supply: 110-230 V
Frequency: 50-60 Hz
Power consumption: Max 100 VA
Fuses 2 x 10A (5x20 mm)
Interfaces
1 USB Host, 1 USB Client, 1 RS232C
Central unity
ARM CORTEX-M4 180 MHz Microprocessor
Safety
Protection category: CLASS I
Safety standards CEI EN 61010-1
EMC: CEI EN 61326
Instrument safety and performance requirements are no longer guaranteed if a cable model other than
the one provided with the kit, which is compatible with the country network voltage of the country of
installation, is used to power the unit.

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2. STORAGE AND TRANSPORTATION
Given the size and weight of the instrument, handling can only be performed by one person.
Chorus Autosampler is a precision instrument and must be handled with care. Improper operation could
damage internal components and cause mechanical damage. For instrument storage and handling,
environmental conditions specified in section 1 must be respected.
Transport must be carried out using all necessary precautions to avoid excessive bumps and inclinations that
could damage the instrument.
Fig. 1 - Full packaging
Packaging dimensions and weight are listed in section 1.
Remember to keep the original full packaging of internal parts for any subsequent instrument
transportation.

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3. PREPARATION AND CHECKS BEFORE INSTALLATION
The following conditions must be met for operator and instrument safety:
1. The power source (installation category II) must be compatible with the electrical and voltage
specifications indicated on the electric power plate supplied on the rear of the instrument; it is
advisable that the efficiency of the electrical system is periodically verified. The mains supply and
sockets must be equipped with an efficient earth connection according to the current electrical
equipment regulations.
2. Before operating connections with external instruments and/or accessories, remember to always
turn the instrument off, checking its compatibility (refer to the user manual).
3. The operator must receive training about procedures, prohibitions and warnings mentioned in this
manual as well as workplace safety.
4. Operator safety equipment (gloves, consumable waste disposal containers, cleaning tool cleaners)
must be available at all times.
5. The instrument must be installed according to the indications at paragraph 5.
6. IT IS FORBIDDEN to remove or modify the instrument's safety and protection devices.

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4. INSTALLATION REQUIREMENTS AND MATERIALS
Autosampler:
1. Chorus Autosampler [Ref. 81210]
2. Installation guide
3. Latest version of the Autosampler firmware available in USB pen drive
4. USB connection cable
5. Power cord
6. Assembly screws and washers
7. Disposable tips (32)
8. Laboratory barcode full sample test tubes
9. Flashlight/torch
10. 2,5 - 3 Allen key
11. Philips screwdriver
Chorus trio:
1. Chorus Trio CPU 2010 [Ref. 81200]
2. Latest available version of Chorus firmware for Chorus + Autosampler system
3. Modified top carter
4. Power cord
5. External barcode reader
6. PC with Windows operating system and "Chorus Manager" app
7. Latest firmware version for Chorus Trio with sampler
8. Strip without label (10)
9. Internal barcode reader upgrade kit (ZEBEX 5111):
“Z5111 Barcode reader group” [Ref. 30214650]
10. Optical Filter Upgrade Kit + Chorus LED 450:
“Optical Filter Kit + Chorus LED 450” [Ref. 30505050]
11. Chorus Upgrade Kit from CPU 2003 To CPU 2010 (only valid for Chorus already TRIO)
“CPU 2010 Kit” [Ref. 30502520]

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5. UNPACKING
Proceed with the unpacking steps as described and shown in the photographic sequence:
1. Cut the adhesive tape sealing the box (indicated by the arrow) with a dedicated tool:
Fig. 2 - Box closed and open
2. Remove the cardboard inner cover:
Fig. 3 - Inner carton part removed
3. Remove the white polyethylene inner part:
Fig. 4 - Autosampler visible in the box

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Now the instrument can be extracted from the packaging. Grab the instrument with both hands, and pull
upwards by avoiding to hold it by the thinner and protruding part (shown in the following figure with the
arrow and label "NO").
Fig. 5 - Details for gripping instrument
4. Check matching between packaging list and the supplied material.
NOTE: If the instrument and/or its accessories were damaged during transportation, please notify the
courier and the Service Centre. If missing, please notify the Service Centre.
Supplied material
The material supplied with the system includes:
1. Autosampler [Ref. 81210]
2. Power cord
3. USB connection cable with Chorus
4. Envelope with assembly screws and washer
5. No. 6 calibrators and positive controls adapters
6. Used disposable tips waste container
NO
SI (YES)

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6. PLACEMENT
Chorus Autosampler is designed to be placed and installed on Chorus Trio, as shown in Fig. 6.
Fig. 6- Chorus Autosampler placement
The table on which Chorus + Chorus Autosampler system is placed must be able to bear the weight of the
two instruments, and it shall not exceed 90 cm in height, to provide the operator with an ergonomically
correct position while entering touch screen controls of the preparation module.
The resulting height chart of the entire system is shown in Fig. 7.
Fig. 7 - System heights chart
In addition, in order to be able to access connectors on the instrument side and, above all, in order to be able
to act promptly, in case of danger, on the switch and power cord, the safety distance from the instrument's
left side to the wall or from other instruments is of 20 cm at least.
For the same reason, it is absolutely forbidden to place any material on top of the instrument.
Choose a location near a power socket that is free from power surges.

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When supplied with Autosampler, Chorus comes with a modified top carter with a module mounting plate.
In case of installing the sampler after the installation of Chorus, the top carter replacement with the modified
one should be carried out. Top carter modification consists of 3 holes, as shown in Fig. 8.
Fig. 8 - Upper and lateral holes
The two holes in the first image are assigned one (B) to the insertion of the motor of the Samples Rack Plate
for Chorus Autosampler (which protrudes from the carter, see Fig. 9) and the other (A) to the input of the
arm with tips for transferring samples to strips in Chorus.
Fig. 9 - Prominent motor
The side hole (C), however, is necessary for part of the procedure of the tip position calibration with respect
to the Chorus strip well in Chorus for samples transfer, as described in section 7.
A
B
C

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7. INSTALLATION
Chorus Autosampler comes with no removable blocks.
For installation proceed as follows:
1. place the instrument on top of the plate described in the previous paragraph, making sure to insert
the motor protruding from the carter into the hole specifically designed for this operation. For more
details refer to Fig. 10.
Fig. 10 - Machine placement
2. Make sure the power switch is turned OFF ("I" sticking out) before proceeding
3. Take the envelope with screws and slotted inserts from the machine kit. The envelope contains 4
pieces per component type: for single components see Fig. 11.
Fig. 11 - Components useful for assembly
4. When opening the front door of the instrument two holes for blocking the instrument together with
Chorus Trio are visible, shown in Fig. 12. One is placed between the used tips waste container and
the rotor, and another one is placed in front of the bar code reader.
A
B
C
D

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Fig. 12 - Instrument stop front holes
5. Place the D component of Fig. 11 on the instrument carter in correspondence of the visible hole with
the slot length parallel to the instrument's side (Fig. 12) and stop the instrument by screwing the A-
B-C components group inserted into each other in this sequence, as shown in Fig. 13.
Fig. 13 - Instrument stop
6. Unscrew the back panel (6 screws of size 2.5) shown in Fig. 14.
Fig. 14 - Back panel

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The screws are on the back of the instrument (4) and one on each side, in the position indicated in Fig. 14.
Fig. 15 - Machine without back panel –Screws and slotted inserts holes
7. It is now possible to see two holes that are placed on two opposite corners of the inner compartment
(see Fig. 15). Secure the instrument on the Chorus plate with screws and slotted inserts, as shown in
the first image of Fig. 15 as per the front holes.
8. In order to complete the installation, the correct robotic arm's pick-up parameters must be set in the
service page. This procedure will be used to adjust the tip position in Autosampler against the strip
well for sample transfer into Chorus. Only after this step the position where the sampler is screwed
to the plate on Chorus can be adjusted and corrected.
Follow these steps:
oTurn the machine on using the switch, which must have the symbol "O" sticking out and wait
a few seconds loading the sw.
oThe following images will appear, as shown in Fig. 16.
Fig. 16 - Launching software

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oPress the key to access the service menu. The first image will appear, as shown in Fig. 17.
Fig. 17 - Access to service menu
Press the arrow on the label “SERVICE”. The second image will appear, as shown in Fig. 17,
containing information about the instrument firmware version and a virtual keypad. Type
the secret code “9-8-5-2-1” and press on the arrow symbol (circled in red). The first page of
the service menu, containing 14 pages, will be displayed. To scroll, simply press the buttons
with the purple arrows, at the bottom right and left .
oGo to 9/14 page and check the proper parameter configuration relative to Z axis position (vertical
arm movement) that is calculated in relation to the samples rack rotor. This value is used as a
benchmark in all software procedures where calculations on the heights useful for sample
suction and level research are found. The value “Top Z” (Fig. 18) to be set, represents the number
of steps the arm needs to make because spindle is about 1 mm from the surface of the samples
rack rotor (Fig. 18).
To set the desired value, press the displayed value of the parameter. The virtual keypad will be
displayed as shown in Fig. 18. Type the desired value or use arrows (single arrow 10 steps,
double-headed arrow 100 steps) to modify and confirm the entry by pressing the lower right
arrow (circled in red).
NOTE: To make this adjustment make sure there are no tips into the rotor
NOTE: All pages in the service menu that contain a settable parameter have the following
features: press the confirmation key to the set value (arrow), arm movement will start
automatically, even in case of confirmation of the value already set.
The same result is achieved by pressing the "ESC" key on the keypad.
To return to the "home" position, move the mechanical arm to page 10/14 and press the key
“UNLOAD”. This action enables the tip release procedure and takes the X axis movement back
to "0".

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Fig. 18 - “Top Z “configuration
oGo to page 3/14 to set the position of the spindle axis that will grab the tip. Place the tips into
the rotor. It is important that the spindle is in the axis with the tip, therefore at the centre of its
diameter, to ensure a correct grip. The tips are distributed on two concentric circles, that is, on
an external row and an internal one with respect to the centre of the sample rack rotor, and
parameters are divided in the same way, on the outer position and on the inner position (Fig.
19).
Fig. 19 - Tip grabbing position setting
Both for “External position” (outer row) and “Internal position” (inner row) the setting parameters
are two: “X Axis” and “Plate”, i.e. movement on the horizontal axis and rotation of the plate.
The first parameter that is set is “X Axis”.
By pressing "T0 Test" the spindle moves to about 1 mm from the edge of the tip to make it visible
whether its position is in the axis or not. It may be necessary to make an adjustment if the axes do
not coincide. Such arm position adjustment occurs with a compromise of steps on the X axis and the
position taken by the sample rack plate, as there is no movement on the Y axis. Thus, steps are taken
(with single arrows for a 10-step change or double arrows for 100) by increasing or decreasing the
value on the X axis, and respectively decreasing or increasing the rotor steps to find the point where
spindle and tip can be considered on the axis. The value can also be inserted with the virtual keypad
that appears as soon as selecting the desired value in order to achieve the correct tip picking position.
For a more in-depth description of this service page, refer to section 8.2.

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To return the mechanical arm to the "home" position, move to page 10/14 and press the key
“UNLOAD”. This action enables the tip release procedure and takes the X axis movement back
to "0".
9. Insert a strip into Chorus in position No.25 with 50 μl in the first well. Connect the Chorus instrument
USB cable to a PC running Windows operating system where the “Chorus manager” application is
installed. Enter the “Chorus Manager” program and follow the steps to take:
Controls -> Hardware Controls-> reset “TSD”, which takes the strip to the barcode reading
position that coincides with the samples transfer position with Autosampler.
10. Perform a grabbing tip test by performing the following steps:
Place tips into the Autosampler rotor.
Enter the service menu as described in step 8
Go to page 10/14 “Take Tip Parameters” and press “TEST” key.
Go to page 2/14 (Fig. 20)
Fig. 20 - Dispensing position
In “Chorus Dispensation Pos” section, there are the two parameters responsible for the
movement of the mechanical arm on X axis, and then towards the Chorus and Z axis transfer
holes, thus vertically, for insertion into Chorus. Both parameters can be edited with the
virtual keypad that appears as soon as you press the value key or the single (10-step) or
double (100-step) arrows. “DETECT” key at this stage should NOT be touched. Tick the
“Movements Enable” box (this automatically enables the arm movement to the hole in
Chorus as soon as the entered value is confirmed), and enter the desired steps in one of the
two directions (if the steps are already the desired ones enter and exit from typing). It is
advisable to set 150 steps less than the ones present in the Z axis (vertical), so as to avoid
unwanted bumps of the tip in the strip.
With this procedure the arm must be carried in an axis position and very close to the strip
well.
11. Unscrew the 4 screws with slotted insert to unlock the instrument.
12. Manually place the instrument on the Chorus screwed plate, so that the tip is centred on the Chorus
well of the strip. It is advisable to use a flash light to illuminate the Chorus interior through the hole
used for the arm input and a camera (of any cell phone) to frame from the side hole shown in Fig. 8
the tip and its position compared o the well No. 1 of the strip.
13. Once you have found the correct position, that is, where the tip is in the centre of the strip well,
screw Chorus Autosampler firmly into Chorus with the help of the components described in step 3.
14. Replace and screw the back panel.

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15. From page 2/14 of the service menu, the second adjustment is made for the vertical axis of the arm,
because the tip must be calibrated not only centrally in relation to well diameter but also at a height
of about 2 mm from its bottom.
Press “DETECT” key. The arm will carry out the following actions:
Set a “Z Axis” value of 100 steps below the average of the instruments
The arm leads to a value of "Z Axis" -200 steps
The arm performs a level search of 400 steps
If a pressure variation is detected in this run that indicates a level, the value to which it was found
will automatically be saved in the "Z Axis" field and the adjustment is deemed to be successful.
If the level is not detected, increase the value in “Z Axis” of 100 steps, and repeat the entire
procedure.
16. To set sample offset parameters (page 4/14) and barcode reader (5/14) machine settings must be
complete (Fig. 29); please refer to sections 8.3 and 8.4.
17. Also check the proper fixed and default parameters of pages 10/14 to 14/14 by comparing with the
images in section 9.
18. Exit the service menu to save the parameters.
19. Connect the USB cable on one side to the Chorus Autosampler (flat part) and on the Chorus on the
other, as shown in Fig. 21.
20. Connect the power cord socket (use the power cord supplied with the instrument) to the socket on
the left side of the instrument (as shown in Fig. 21). Connect the power cord to the mains. Repeat
the same for Chorus.
21. Turn on the two machines by moving the switch to the position where the letter "O" is protruding.
22. Close the Autosampler side hole with the cover with screws, as shown in Fig. 22.
Fig. 21 - Power outlet + USB

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Fig. 22 - Side hole cover

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8. FUNCTIONAL STARTING PROCEDURE
To set the instrument parameters it is necessary to enter the pages of the service menu. Take the following
steps:
1. Turn the machine on using the switch, which must have the symbol "O" sticking out
2. Wait a few seconds while the sw is loading
3. The following images will appear, as shown in Fig. 23.
Fig. 23 Launching software screen
4. Press the key to access the service menu. The first image will appear, as shown in Fig. 24.
Fig. 24 - Service access
5. There are three parameters:
TEMP. SCALE: Temperature Scale Type -> NOT USED
DATE/TIME: Pressing the arrow it is possible to enter a page where you can view the date
and time saved in the instrument. From the same page you can also set these two items by
using the “Date set” and “Time set” keys.
SERVICE: Pressing the arrow corresponding to this parameter it is possible to enter the
service menu. The second image shown in Fig. 24 will appear, containing information about
the instrument firmware version and a virtual keypad. Type the secret code “9-8-5-2-1” and
press on the arrow symbol (circled in red). The first page of the service menu, containing 14

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pages, will be displayed, as shown in Fig. 25. To scroll, simply press the buttons depicting the
purple arrows, at the bottom right and left.
6. To exit the service, press the green arrow at the left bottom. When a parameter is changed, to be
saved, it is also necessary to exit the service menu.
Fig. 25 - First page service menu
PAGE PARAMETERS
PARAMETER
USE
SN
Machine serial number
TEMP ADJ
Not used
SD MANAGEMENT
Not usable
Door Check Disable (Y/N)
Disables electromechanical door control that is
always open.
DEFAULT: NO
When the instrument is turned off, if you have
chosen to disable this control, the status will
return to the default setting.
FUNCTIONS
FUNCTION
Door Lock
OPEN/CLOSE: it opens and closes the door by
acting on the solenoid.