Diesse VES-MATIC 30 User manual

VES-MATIC 30/30 PLUS
INSTRUCTIONS MANUAL
Software Release SW 1.38
Rev. 2.0 issued on February 2016
Automatic instrument for the determination
of the Erythrocyte Sedimentation Rate (ESR)
(Patented)
For In Vitro Diagnostic use only

Instructions Manual
2/62 Rev.2.0 of 02/2016
MANUFACTURER
DIESSE DIAGNOSTICA SENESE S.p.A.
Via delle Rose 10, 53035 Monteriggioni (SI), Italy
Tel. [phone] Fax. [phone]
www.diesse.it
LEGAL REPRESENTATIVE
PRESIDENT
Dr. Francesco Cocola
REGISTERED AND ADMINISTRATIVE OFFICE
Via A. Solari 19, 20144 MILANO, Italy
Tel. [phone] Fax. [phone]
For Technical Assistance please contact your distributor:
The information contained in this manual may be subject to modifications without notice. No page in this
manual may be reproduced in any form or by any means, neither electronic nor mechanical, for any use
whatsoever without prior written permission from DIESSE Diagnostica Senese S.p.A.

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Standard applied in this document:
- 98/79/EEC “directive relevant to the In Vitro Medical-Diagnostic Devices (IVD)”
- EN 61010-1 “Safety requirements for electrical equipment for measurement, control
laboratory use –Part1: General requirements”.
- EN 61326-1 “Electrical Equipment for Measurement, Control, and Laboratory Use –
Electromagnetic compatibility requirements-Part 1: General requirements”
- EN 61326-2-6 “Electrical Equipment for Measurement, Control, and Laboratory Use –
Electromagnetic compatibility requirements-Part 2-6: In Vitro Diagnostic (IVD) medical
equipment”
- 2006/95/EEC “Low Voltage Directive”
- 2004/108/EEC “Electromagnetic Compatibility Directive”
- 2011/65/EU “Restriction of Hazardous Substances in Electrical and Electronic Equipment
Directive –RoHS2”
LIST OF REVISION
MANUAL
REVISIONS
VERSION SW
VES-MATIC30
DESCRIPTION OF MODIFICATION
0.00
of
04/01
1.08
of
04/01
Initial version
0.01
of
06/01
1.13
of
06/01
Initial revision with photos
0.02
of
07/01
1.16
of
07/01
Paragraph inserted in Manual Insertion ID number.
0.03
of
09/01
1.22
of
09/01
Description of Check Device updating
0.04
of
06/02
1.31
of
06/02
Clarifications on new type of serial protocol
0.05
of
07/02
1.32
of
07/02
Compliance of chap. 4 –6 - 7 with SW.1.32 and the addition of
attachment F
0.06
of
09/02
1.32
of
09/02
Points 1.7,2.4, 2.8, 3.2, 5.5 and graphic symbols revised [Ref.
EN61010-1 (Ed. 2001 standard)]
0.07
of
03/03
1.32
of
03/03
Paragraphs 1.2. (Guarantee) & 2.5. (Power supply cable
specifications) revised
1.00
of
10/03
1.38
of
12/03
Official version of IVDD/CE
1.10
of
10/14
1.38
of
12/03
Added Intended Use, removed attachment B, Updated attachment A
2.00
of
04/15
1.38
of
12/03
Updated chapter 1, changed attachments and complete restyling.
MODELS
This manual applies to the following VES-MATIC 30/30 PLUS models:
DIESSE Code
Description
10354/CE
VES-MATIC 30
10356/CE
VES-MATIC 30 Plus

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SYMBOLS
Legend of graphic symbols used on the instrument.
Instrument satisfying requirements of European directive on in vitro diagnostic
medical devices (98/79/EC).
In Vitro Diagnostic medical device.
Date of manufacturing of the unit.
Serial number of the unit.
Manufacturer.
Legend of Electrical and Safety symbols used on the instrument.
Protective conductor terminal.
WEEE: - Obligation of separate waste collection according to L.D. 25/07/2005 no.
151 (Italy), enforcement of the 2002/96/EC and 2003/108/EC directives.
Warning, read the Manual and pay attention to the safety symbols.
Warning, risk of electric shock.
Legend of symbols used in this document.
ATTENTION, potential hazard of personal injuries, all conditions indicated in the
text have to be read and understood before proceeding.
CAUTION, potential danger of damage to the machine, all conditions indicated in
the text have to be read and understood before proceeding.
NOTE, important information
BIOHAZARD, danger of contamination with possibly infected materials.
Instrument satisfying CSA standards for the Canadian and U.S.A. market.

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GENERAL WARNINGS
Before installation and use of the instrument, for ensuring correct and safe use, it is recommended
carefully reading the warnings and instructions contained in this instruction manual.
It is important for this instruction manual always to be stored together with the instrument for future
consultation.
In case of selling or transferring the instrument, make sure that the manual always accompanies VES-
MATIC 30/30 PLUS to allow the new owner to be informed about its functioning and relative warnings.
The instrument should be used only by qualified and competent personnel.

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CONTENTS
CHAPTER 1.............................................................................................................................................8
1.0 INTENDED USE OFTHE INSTRUMENTS ...................................................................................8
1.1 PRESENTATION OF THE INSTRUMENT ....................................................................................8
1.2 GENERAL DESCRIPTION OFTHE INSTRUMENT....................................................................10
1.3 MATERIAL SUPPLIED WITH THE INSTRUMENT......................................................................11
1.4 LIST OF MATERIAL REQUIRED BUT NOT PROVIDED.............................................................11
1.5 TECHNICAL SPECIFICATIONS.................................................................................................12
1.6 BLOCK DIAGRAM......................................................................................................................13
1.7 TECHNICAL DESCRIPTION OF THE INSTRUMENT.................................................................13
1.8 INFORMATION REGARDING DISPOSAL..................................................................................14
CHAPTER 2...........................................................................................................................................15
2.1 STORAGE AND TRANSPORT ...................................................................................................15
2.2 PREPARATION AND CHECKS PRIOR TO INSTALLATION.......................................................15
2.3 UNPACKING ..............................................................................................................................16
2.4 POSITIONING............................................................................................................................17
2.5 INSTALLATION..........................................................................................................................18
2.6 RESTRICTIONS ANDWARNINGS.............................................................................................18
2.7 SHUTTING DOWN AND SHIPPING ...........................................................................................19
3.1 TURNING ONTHE INSTRUMENT .............................................................................................20
3.2 KEYBOARD................................................................................................................................20
3.3 MAIN MENU ...............................................................................................................................21
3.4 DESCRIPTION OFTHE EXAMS.................................................................................................23
3.4.1 ESR ANALYSIS..................................................................................................................23
3.4.2 FAST ESR ANALYSIS........................................................................................................25
3.4.3 ESR KINECTIS ANALYSIS.................................................................................................27
3.5 DISPLAYING (AND PRINTING) OFTHE STORED ANALYSIS...................................................28
3.6 SET-UP ......................................................................................................................................30
3.6.1 CLOCK ...............................................................................................................................31
3.6.2 LANGUAGE........................................................................................................................31
3.6.3 TEMPERATURE SCALE ....................................................................................................31
3.6.4 [PARAMETER] SETTING ...................................................................................................32
A. TEMPERATURE [CORRECTION].......................................................................................32
B. DISPLAY.............................................................................................................................32
C. PRINTER ............................................................................................................................32
D. INVERT PRINTER ..............................................................................................................33
3.6.5 QUALITY CONTROL SET-UP ............................................................................................33
CHAPTER 4...........................................................................................................................................35
4.1 TURNING ON FOR THE FIRST TIME.........................................................................................35
4.2 PREPARING THE SAMPLE........................................................................................................35
4.3 SEQUENCE FOR PREPARING AN EXAM.................................................................................36
4.3.1 ACQUISITION OF THE IDENTIFICATION NUMBER (Default set-up for the models with the
onboard Barcode Reader) ..............................................................................................................37

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4.4 SEQUENCE FOR PREPARING AN EXAMWITH THE LOADING OFTHE ID NUMBER FROM
THE KEYBOARD OR WITH EXTERNAL BARCODE READER ...........................................................39
CHAPTER 5...........................................................................................................................................44
5.1 GENERAL RECOMMENDATIONS.............................................................................................44
5.2 EXTERNAL CLEANING OF THE INSTRUMENT ........................................................................44
5.3 REPLACING THE PAPER IN THE PRINTER..............................................................................44
5.4 REPLACEMENT OFTHE CHECK DEVICE ................................................................................45
5.5 PERIODIC CHECKS...................................................................................................................46
5.5.1 CHECK OF THE ACOUSTIC AND SAFETY DEVICES (MICROSWITCHES).....................46
5.5.2 CHECK OF THE SPRINGS.................................................................................................46
5.6 REPLACEMENT OFTHE FUSES (UNSCHEDULED MAINTENANCE) ......................................46
CHAPTER 6...........................................................................................................................................48
6.1 SELF-DIAGNOSIS......................................................................................................................48
7.1 EXTERNAL BARCODE READER...............................................................................................50
GENERAL CONNECTION SPECIFICATIONS...............................................................................50
7.2 CONNECTING UP TO AHOST COMPUTER..............................................................................50
GENERAL SPECIFICATIONS FOR THE RS232C SERIAL CONNECTION...................................51
GENERAL SPECIFICATIONS FOR THE RS485 SERIAL CONNECTION......................................51
7.3 SPECIFICATIONS OFTHE ASYNCHRONOUS SERIAL COMMUNICATION PROTOCOL........51
7.4 “NEW PROTOCOL” SPECIFICATIONS......................................................................................51
VES20/30 SERIAL COMMANDS AND PARAMETER FORMAT.....................................................52
BIBLIOGRAPHY....................................................................................................................................58
ATTACHMENT A: CE COMPLIANCE CERTIFICATE...........................................................................59
ATTACHMENT B: GUIDELINES FOR THE INSTALLATION REPORT ................................................59
ATTACHMENT C: OPTIONAL “ CONVERTER KIT - USB/485” ( ORDER CODE: 30501150).............60

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CHAPTER 1
1.0 INTENDED USE OF THE INSTRUMENTS
Automatic instrument for the determination of erythrocyte sedimentation rate (ESR)
1.1 PRESENTATION OF THE INSTRUMENT
VES-MATIC 30/30 PLUS is a bench instrument designed and programmed to simultaneously determine
the erythrocyte sedimentation rate (ESR) in a maximum of 30 samples of blood (human or animal),
contained in dedicated cuvettes.
Cuvettes compatible with VES-MATIC 30/30 PLUS :
VES-TEC - (refer to relative
Instructions For Use).
Non evacuated tubes to be
filled manually, containing
sodium citrate solution for
direct determination of the
ESR. Sample volume ≈ 1 ml.
CODE:
- 10201/A
- 10201/W
- 10214
- 10212
- 10210
VACU-TEC - (refer to relative
Instructions For Use).
Evacuated tubes for direct
blood drawing, containing
sodium citrate solution for
direct determination of the
ESR. Sample volume ≈ 1 ml.
CODE:
- 10200
- 10202
- 10200/W
- 10202/W
- 10208
- 10209
- 10207
- 10206
- 10600
- 10602
- 10238
- 10235
- 10200/S
10230
- 10232
- 10220
VACU-CODE (refer to
relative Instructions For Use).
Evacuated tubes for direct
blood drawing, containing
sodium citrate solution for
direct determination of the
ESR.
Sample volume ≈ 1 ml.
CODE:
- 10230
Fig. 1.1.1
The instrument is managed by a microprocessor the functioning of which will be described in more detail
in the following paragraphs.
The analysis is performed completely autonomously (mixing and reading) and the results are
comparable with those obtained with the Westergren method1-10; and if obtained at room temperature
they can be taken up to a temperature of 18°C in automatic mode according to Manley’s Nomogram11.

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VES-MATIC 30/30 PLUS allows for obtaining results equivalent to those of the Westergren method
(1st hour) in only 28 minutes, and equivalent to the Westergren method (2nd hour) in only 48 minutes,
including the mixing of the sample. In the fast cycle, the times will be reduced to 12 minutes for the first
hour and 17 minutes 30 seconds for the second hour.
The instrument is designed with the temperature
correction always activated and relates the results to a
temperature of 18°C according to Manley’s
Nomogram11.
However, it is possible to de-select the temperature
correction for individual laboratory needs.
Clinical concept of the ESR
The Erythrocyte Sedimentation Rate test (ESR) is performed measuring the distance travelled by red
cells in autologous plasma over a fixed period of time. In normal conditions, red cells tend to repel each
other by virtue of the negative membrane net charge, due to the presence of numerous sialic acid
residues at the level of membrane glycoproteins.
When the protein composition of the plasma is changed, with the production of so-called "acute phase
proteins", as a result of an inflammatory process or tissue damage, thanks to the binding of these
proteins (fibrinogen, immunoglobulins) to the surface of red blood cells, the negative charge of
membrane potential (Z) is altered and the red blood cells can pile-up, to form the so-called rouleaux,
which then aggregate to form microspheres of uniform radius, that begin to sediment when their density
exceeds that of the plasma in which they are immersed. The value of the ESR is then increased in all of
those situations in which there is an increase in acute phase proteins and in particular fibrinogen (which
is thought to contribute for 70% to the total phenomenon of sedimentation) and immunoglobulins (that
will increase in case of onco-haematological diseases and acute infections). ESR is therefore an indirect
not specific measure of an inflammatory state and is elevated in various pathological conditions such as
inflammatory diseases (infections, rheumatic diseases), relative/absolute increase of globulins (nephrotic
syndrome, myeloma), tissue necrosis (myocardial infarction, tumors). ESR is useful for predicting the
prognosis of some diseases such as polymyalgia rheumatica, giant cell arteritis, rheumatoid arthritis and
Hodgkins disease and is useful as a marker of treatment efficacy in many diseases such as rheumatoid
arthritis, vasculitis, collagenosis, septic arthritis.
The erythrocyte sedimentation rate is usually higher in females than males and is increased in
pregnancy and it has also a tendency to increase in both sexes with increasing age.
In the classical formulation of Westergren the test is performed on blood anti-coagulated with citrate, in
the proportion of 4 parts blood to one part of anticoagulant. The diluted blood is then aspirated inside a
special graduated pipette with inner diameter of 2.5 mm held vertically in a stand; the level of
sedimentation of the erythrocytes is recorded after one hour, measuring the distance between the lower
face of the meniscus of the plasma and the upper face of the layer of sedimented red blood cells.
However, in order to make the measurement of the ESR according to Westergren’s technique follow the
recommendations of the International Committee for Standardization in Haematology (ICSH)12.

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Normal ESR
According to the scientific literature, normally ESR values are between 1 and 10 mm/h for males and
between 1 and 15 mm/h for females; in pathological conditions they can increase to values of up to 100
mm/h and above.
Approximate normal range of VES-MATIC 30/30 PLUS instrument:
MALES
up to 10 mm first hour
up to 20 mm second hour
FEMALES
up to 15 mm first hour
up to 30 mm second hour
These values have to be considered as entirely approximate and vary in function of age and gender.
According to international guidelines, each laboratory should determine its own normal ranges based on
gender and divided by decades of age.
General functioning of the instrument:
The blood collected in the relative cuvettes is carefully mixed by the instrument; then the samples are left
to stand for a preset period in order for the sedimentation to take place.
By means of a digital sensor (opto-electronic unit) the instrument automatically determines the
sedimentation level of the erythrocytes, obtained results are processed and then either automatically
printed or shown on the display.
The analytical results are calculated from the values set on the instruments and obtained via the
Westergren reference method.
Please note that in the case of samples with a hematocrit value <15% the instrument will print the
message “(Sample) EMPTY”.
1.2 GENERAL DESCRIPTION OF THE INSTRUMENT
Fig. 1.2.1 Front view
Legend:
1) Keyboard
2) Display
3) Paper-holder compartment with printout slit
4) Lid of the sample-holder compartment
5) Cuvette-holder plate with 30 positions
6) Numbered cuvette-holder positions
7) Slot for replaceable Check Device (Check
Card)

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Fig. 1.2.2 Rear view
Legend:
1) ON/OFF switch
2) Fuses/Voltage modifier
3) Socket for power supply
4) RS232 connector (for hooking up to
an external computer)
5) BC EXT connector (for an external
optical barcode reader)
6) RS485 connector
7) Forced air fan
1.3 MATERIAL SUPPLIED WITH THE INSTRUMENT
VES-MATIC 30/30 Plus is supplied with the following material:
Instruction manual (CD) [Order Code: P30650010]
2x1.0A T fuses (5x20mm) [Order Code: P20400000]
IEC International Standard* power supply cable [Order Code: P21890040]
Packing-list & Installation report
Compliance Certificate
Guarantee Certificate
*[Features: Female Plug IEC 320 C-13; Male Plug Schuko EEC 7-VII; Rating: 10A/250Vac]
1.4 LIST OF MATERIAL REQUIRED BUT NOT PROVIDED
Dispensable pipettes only for VESTEC cuvettes
Holder
Disposable gloves
Cleaning paper
Solutions for cleaning the instrument
ESR tubes (consult Fig. 1.1.1)
ESR Control 4 x 9 ml (2 x Level I + 2 x Level II) REF 10430
ESR Control 2 x 9 ml (1 x Level I + 1 x Level II) REF 10434

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1.5 TECHNICAL SPECIFICATIONS
Power supply
from 90 to 264 VAC (50 / 60 Hz)
Power consumption
65 W max
Fuses
2 x 1.0A T (5 x 20 mm)
Dimensions
510 x 350 x 500 mm ( l x h x p )
Weight
20 Kg
Ambient temperature
Operational
from +15 to +35°C
Storage
from + 5°C to + 45°C
Relative humidity tolerance
from 20 to 80% without condensation
Central unit
RISC 8 BIT technology with extremely low dissipation; ATMEGA103
microprocessor
Display
240x128 pixel liquid crystal display rear-illuminated with a CFL lamp.
Cuvette-holder plate
With 30 numbered recesses for the relative cuvettes.
Plate rotation speed
1 rotation every 1.5 seconds during normal operation. In the ESR fast
cycle at 240 RPM.
Optical unit
A pair of opto-electronic elements in solid state (photodiode +
phototransistor).
Printer
Alphanumeric with thermal paper 58 mm wide, 36 characters per line,
speed: 20 mm/sec.
Interfaces
RS232C and RS485
Protection category
CLASS I
Device safety
EN61010-1
EMC
2004/108/EEC “Electromagnetic Compatibility Directive”
Installation category
II

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1.6 BLOCK DIAGRAM
(Detailed diagrams are contained in the Assistance Manual)
Fig.1.6.1 Block diagram –VES-MATIC 30/30 Plus
LEGEND:
1. C.P.U. board
2. Motor Driver board
3. Power supply
4. Motor unit
5. Reading sensor unit
6. Check-Device unit
7. Display/Keyboard interface
8. Display
9. Keyboard
10. Printer interface
11. Printer
12. Solenoid valve unit door
13. Micro-switch door
14. Forced air fan
15. ON/OFF switch with socket filter
16. External connections
17. Internal barcode reader
1.7 TECHNICAL DESCRIPTION OF THE INSTRUMENT
CENTRAL UNIT
Controls and processes the data arriving from the sensors and manages connected peripherals,
including the FLASH EPROM containing the programme. It also contains the EEPROM where all the
instrument parameters are stored.
OPTICAL READING UNIT
Unit consisting of a photodiode + phototransistor couple.

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MOTORS FOR TIPPING AND ROTATING THE CUVETTE-HOLDER PLATE
One motor carries out the tipping of the cuvette-holder plate to 90° and another makes it rotate by means
of a shaft, in order to guarantee a homogeneous suspension of the red blood cells.
OPTICAL READER MOTOR UNIT
Takes care of lifting the optical reader unit in order to check the presence of cuvettes and/or control their
correct placing, proper blood level and detection of the sedimentation level.
KEYBOARD
Consists of 5 keys used to implement all VES-MATIC 30/30 Plus functions.
CUVETTE-HOLDER PLATE
The plate has a radial crown with 30 numbered recesses for inserting the cuvettes. These are
symmetrically tilted with respect to the rotation axis in order to arrange the cuvettes on a cone generator.
ACOUSTIC SIGNAL
Serves the purpose of attracting the operator’s attention during specific phases of the work cycle.
It emits a "beep" sound after the keys are pressed on the keyboard.
TEMPERATURE PROBE
Measures the temperature and is located on the central unit near the cuvette-holder plates.
PRINTER
Prints the results of the analysis at the end of each processing cycle.
DISPLAY
Displays all the messages of the instrument.
1.8 INFORMATION REGARDING DISPOSAL
VES-MATIC 30/30 Plus is composed of the following (with their percentages):
IRON
20%
ALUMINIUM
20%
COPPER
8%
EPOXY RESINS and SILICON
6%
PLASTIC MATERIALS
46%
VES-MATIC 30/30 Plus instrument is operated by mains voltage, therefore has been classified as
Electrical-Electronic Equipment according to the European Directive 2012/19/UE dated July 04 2012,
and subsequent amendments.
At the end of its life cycle, the instrument must be treated as separate collection, potentially infected,
waste and eliminated according to law.

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CHAPTER 2
2.1 STORAGE AND TRANSPORT
VES-MATIC 30/30 Plus is a precision instrument and must be treated as such.
Inappropriate operations could damage the internal opto-electronic components and cause
mechanical damage.
In view of the overall dimensions and weight of the machine, the transport must be carried out manually
by taking all necessary precautions in order to avoid impact or excessive tipping over that could damage
the instrument.
Fig.2.1.1 Complete packaging
Package dimensions
WIDTH (box)
cm
65
HEIGHT (box)
cm
50
DEPTH (box)
cm
70
GROSS WEIGHT
kg
26
PACKAGING WEIGHT
kg
6
Do not throw away the original packaging of the internal parts of the instrument in case of
further transportation of the instrument in the future.
2.2 PREPARATION AND CHECKS PRIOR TO INSTALLATION
In order to ensure the safety of the instrument and the operator the following conditions must be
guaranteed:

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The power supply system (installation category II), must be “compatible” with the voltage
and current specifications indicated on the nameplate attached at the back side of the
instrument; and it is recommended to check the efficiency of the electrical system
periodically.
The electrical system and relative sockets must all be fitted with efficient ground
connections according to the regulations in force with regard to electrical installations.
The instrument is fitted with an internal power supply that supports voltage surges as per
the specifications indicated on the nameplate at the back side of the device; in the event of
the line being very disturbed, use a saturated iron stabiliser (65 W minimum) for minimising
the power surges.
Before making connections with external instruments (PCs, Barcode readers), it will be
necessary to check the compatibility (refer to the relative instruction manuals) with the
specifications indicated in chapter 7 and check that there is continuity between the
grounding connection and the external instruments.
The operator must be trained to be familiar with the procedures, restrictions and warnings
indicated in this manual, as well as those pertaining to safety in the work place.
The material for the operator’s safety (gloves, containers for disposing of used expendable
items, detergent solutions for cleaning the instrument) must always be available.
The positioning of the instrument must comply with the instructions indicated in paragraph
2.5.
IT IS STRICTLY PROHIBITED TO:
- cover the rear grids (fan) of the instrument
- remove or modify the safety and protection devices
2.3 UNPACKING
During the handling of the device, avoid impact and excessive tipping over that could
damage the instrument.
Unpacking the instrument.
a. Open the box from the top (see packaging label) and remove the polyethylene mould that holds the
upper section of the instrument in place.
Fig.2.3.1

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b. Remove the manual and the accessories contained on the sides of the instrument.
Fig.2.3.2
c. Lift the instrument out of the box.
d. Remove the protective bag wrapped around the instrument.
e. Check that the material supplied complies with the packing-list.
In case when supplied instrument and/or accessories has been damaged during
transportation notify the Transport Company and Technical Assistance.
In case of any missing items notify Technical Assistance.
2.4 POSITIONING
The laboratory of analysis is the best site for the installation of this instrument.
For normal safety requirements and in view of the type of examination to be performed, the instrument
must be positioned away from heat sources, in areas that cannot be reached by liquids, in dust-free
environments, on perfectly clean, level surfaces that are not subjected to shocks or vibrations.
It is recommended to place the instrument as far away as possible from any generators of
electromagnetic waves (e.g. refrigerators, laboratory centrifuges) and instrumentation lacking in the CE
mark, as these could compromise its correct operating.
In order to avoid overheating of the instrument (blocking of the fan) and to be able to act quickly on the
switch and power supply cable in the event of hazards; the safety distances described in the figure must
be respected (rear panel 20 cm from the wall, front panel free, left and right side panels 10 cm space).
Fig.2.4.1 Installation of VES-MATIC 30 / 30 Plus
It is strictly prohibited to place anything on top of the instrument.
10 CM
10 CM
20 CM

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Choose a position near a power point, which is free from interference and voltage surges.
Never move the instrument while in use. If moving of the instrument is necessary it is
compulsory to recheck the conditions listed in this paragraph before turning it on again. If
the instrument is not used for longer periods of time it should be disconnected from the
power supply and covered.
2.5 INSTALLATION
The installation must be performed by an authorised DIESSE Diagnostica Senese S.p.A. technician as
confirmed by the Installation Report.
1. Place the instrument on a solid surface as described in the previous paragraph.
2. Open the door of the printer compartment and remove the elastic band holding the roll of thermal
paper in place (used to prevent it from coming out of its housing during transport).
3. Open the lid of the sample-holder compartment and remove the adhesive tape used to keep the
plate still during transport.
4. Tilt the instrument on a side. Unscrew the screw on the bottom of the instrument (in the center)
indicated by the yellow label (Fig.2.5.1).
Fig.2.5.1
Screw completely to lock the internal plate before every transportation or shipment.
5. After following the instructions in paragraph 2.2., connect the instrument to the power supply:
Make sure that the power supply switch is in the OFF position « 0 » before proceeding. Connect the
power supply plug (use exclusively the power supply cable supplied with the instrument) to the
socket located at the right side of the switch of the instrument (as indicated in Fig. 2.5.2). Connect
the plug of the power supply cable to the power point.
Fig. 2.5.2
2.6 RESTRICTIONS AND WARNINGS

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IN CASE OF FIRE OR HAZARDS IN GENERAL, TURN OFF THE INSTRUMENT AND
DISCONNECT THE POWER SUPPLY CABLE.
DISCONNECT the instrument from the power supply system before performing any type of
technical operation or in case of malfunctioning of the instrument.
It is only possible to enter the commands with a light pressure of the fingers.
It is prohibited to carry out OPERATIONS on the instrument while there are parts still in
movement (it is only possible to enter the commands on the keyboard).
It is prohibited to PLACE your fingers or other objects between the plate and the safety
guard of the instrument.
It is strictly prohibited to manually TRANSLATE the plate (from the sample-introducing
position to the mixing position and vice-versa).
Expendable materials
RESTRICTIONS
The DIESSE cuvettes are especially designed for use with VES-MATIC 30/30 Plus and cannot
be substituted with any other type of material or supply.
The use of other types of materials may seriously compromise the performance of this
instrument.
DIESSE Diagnostica Senese S.p.A. declines all liability for the performance of the instrument
in case of use of other materials.
All the ESR cuvettes supplied are disposable and must not be reused.
All the ESR cuvettes supplied must be disposed of in compliance with the local laws in force.
Treatment of potentially infected material.
All precautions pertaining biological risks must be adopted when using VES-MATIC 30/30
Plus.
The expendable material must be disposed of in compliance with the laboratory regulations
and the local laws.
Observe all the individual and collective safety laws envisaged for the operator in line with the
working environments. Observe the DIRECTIVES relating to safety and the laws in force.
2.7 SHUTTING DOWN AND SHIPPING
Before shutting down and shipping the instrument it is recommended carrying out the
following sanitising procedure:
a. The instrument must be turned off and cleaned internally of all residues or spillage with a
liquid detergent and left to dry.
b. Use one of the ready-to-use spray cans of isopropyl alcohol available on the market.
c. Spray well inside the instrument and on top of the sample-holder plate.
d. Close the lid of the instrument and leave turned off for at least one hour before beginning
a new work cycle or any other type of operation on the instrument.
During handling avoid all impact or excessive tipping over that could damage the instrument

Instructions Manual
20/62 Rev.2.0 of 02/2016
CHAPTER 3
3.1 TURNING ON THE INSTRUMENT
Turning on
After the installation of the instrument as indicated in Chapter 2, make sure that the lid is closed and turn
the power supply switch (located to the left of the power supply cable at the back side of the instrument)
to the ON position « I ».
Starting up the System
On starting up the instrument carries out an initial Check of:
-The functioning of all the internal units and that the moving parts are in their respective standby
positions.
During the movement of the mechanical parts a safety device will keep the door locked.
The door will only be released at the end of the cycle, at the end of the movement, or
during the procedure for entering the Identification number with the external barcode
reader. To open the lid during the cycle it is first necessary to interrupt the analysis by
means of the specific procedure (ESC key).
-The presence of a new check-device (see paragraph 5.4)
During the initial checks the Software Version installed on the unit will be indicated on the display:
Fig. 3.1.1
3.2 KEYBOARD
Fig. 3.2.1
VES-MATIC 30/30 Plus is fitted with a Control Panel (Fig. 3.2.1) that allows easy access to all the
programmes controlled by the software of the instrument. A LCD with numerous windows guides the
user through all the procedures.
Each key on the control panel corresponds to a virtual button visualised at the bottom of the display. The
function of each key will vary according to the menu page viewed at that time. To enable a function it will
be necessary to press and release the relative key.
WAIT….
Ves30 1.35 24/04/03