Endress+Hauser Analytik Jena PlasmaQuant MS User manual

Operating Manual
PlasmaQuant MS product family
Inductively Coupled Plasma Mass
Spectrometer (ICP-MS)

Analytik Jena AG
Konrad-Zuse-Str.1
07745 Jena Germany
Phone + 49 3641 / 77 70
Fax + 49 3641 / 77 92 79
Email [e-mail]
Analytik Jena AG
Konrad-Zuse-Str. 1
07745 Jena Germany
Phone + 49 3641 / 77 74 07 (Hotline)
Email [e-mail]
Email [e-mail]
For a proper and safe use of this product follow the instructions.
Keep the operating manual for future reference.
http://www.analytik-jena.com
PlasmaQuant is in Germany a registered trademark of Analytik Jena AG. Leybonol is a
registered trademark of OC Oerlikon Corporation AG. Microsoft and Windows are
registered trademarks of Microsoft Corp.
The identification with ® or TM is omitted in this manual.
10-5000-002-23
05.19
Analytik Jena AG
© Copyright 2020, Analytik Jena AG
Manufacturer
Service
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PlasmaQuant MS product family Contents
3
Contents
1Basic information ....................................................................................................... 9
1.1 User manual notes............................................................................................................ 9
1.2 Intended use ................................................................................................................... 10
2Safety instructions.................................................................................................... 11
2.1 General notes.................................................................................................................. 11
2.2 Safety markings on the PlasmaQuant MS .................................................................... 11
2.3 Requirements for operating personnel ......................................................................... 12
2.4 Safety instructions, transport and commissioning ....................................................... 12
2.5 Safety instructions - operation ...................................................................................... 13
2.5.1 General............................................................................................................................ 13
2.5.2 Safety instructions relating to ambient conditions....................................................... 14
2.5.3 Safety instructions - electrical equipment..................................................................... 14
2.5.4 Hazards caused by plasma operation............................................................................ 14
2.5.5 Behavior in case of ring plasmas ................................................................................... 15
2.5.6 Safety instructions relating to the formation of ozone and toxic vapors.................... 16
2.5.7 Safety instructions relating to compressed gas containers and systems .................... 16
2.5.8 Handling of samples, auxiliary and operating materials ............................................. 17
2.5.9 Safety instructions relating to cleaning and decontamination measures................... 18
2.6 Behavior during emergencies ........................................................................................ 18
2.7 Safety instructions - maintenance and repair............................................................... 19
3Function and setup of the PlasmaQuant MS ........................................................... 20
3.1 Physical principle of ICP-MS .......................................................................................... 20
3.2 Setup of the PlasmaQuant MS....................................................................................... 21
3.2.1 Sample introduction system .......................................................................................... 22
3.2.2 Plasma compartment ..................................................................................................... 24
3.2.3 Mass analyzer ................................................................................................................. 28
3.2.4 PlasmaQuant MS – at a look.......................................................................................... 30
4Installation and commissioning ............................................................................... 31
4.1 Installation conditions.................................................................................................... 31
4.1.1 Environmental conditions.............................................................................................. 31
4.1.2 Power supply................................................................................................................... 32
4.1.3 Gas supply ....................................................................................................................... 33
4.1.4 Exhaust unit.................................................................................................................... 34
4.1.5 Water cooler.................................................................................................................... 34
4.1.6 Fore-line roughing pump............................................................................................... 35
4.1.7 Device layout and space requirements.......................................................................... 36
4.2 Supply and control connections..................................................................................... 39
4.3 Installing the PlasmaQuant MS..................................................................................... 42
4.3.1 Installation of the torch.................................................................................................. 43
4.3.2 Installation of the nebulizer........................................................................................... 45
4.3.3 Installation of the spray chamber.................................................................................. 46
4.3.4 Installation of the transfer tube..................................................................................... 47
4.3.5 Installation of the peristaltic pump tubing ................................................................... 50
5Operation.................................................................................................................. 53
5.1 Starting ASpect MS software ......................................................................................... 53
5.2 Turning on power to the PlasmaQuant MS .................................................................. 53
5.3 Starting the vacuum system........................................................................................... 54
5.4 Igniting the plasma......................................................................................................... 55
5.5 Tuning solution preparations ........................................................................................ 55
5.6 Warming up the PlasmaQuant MS................................................................................ 56
5.7 Shutting down the PlasmaQuant MS............................................................................ 57
5.7.1 Putting the PlasmaQuant MS into standby mode........................................................ 57
5.7.2 Complete shutdown of the PlasmaQuant MS (Power Off).......................................... 57

Contents PlasmaQuant MS product family
4
6Optimizing hardware ............................................................................................... 58
6.1 Instrument Automated Setup ........................................................................................ 59
6.2 Torch alignment.............................................................................................................. 59
6.3 Peak resolution and trim................................................................................................ 60
6.4 Mass calibration.............................................................................................................. 62
6.5 Detector voltage.............................................................................................................. 63
6.6 Detector attenuation ...................................................................................................... 64
7Method development............................................................................................... 65
7.1 Create new worksheet.................................................................................................... 65
7.2 Select elements and isotopes......................................................................................... 67
7.3 Calculate isotope ratios .................................................................................................. 69
7.4 Optimize method settings.............................................................................................. 70
7.5 Set up calibration standards........................................................................................... 70
7.6 Enter scan settings.......................................................................................................... 71
7.7 Configure sampling settings........................................................................................... 72
7.8 Configure QC Tests.......................................................................................................... 73
7.9 Enter notes and save method parameters .................................................................... 74
8Method optimization ............................................................................................... 75
8.1 Instrument initialization and solution preparations..................................................... 76
8.2 Manual optimization ...................................................................................................... 76
8.2.1 Copy and paste optimization settings ........................................................................... 76
8.2.2 Scan settings for method optimization ......................................................................... 78
8.2.3 Perform manual method optimization.......................................................................... 78
8.3 Automatic optimization.................................................................................................. 79
8.3.1 Copy and paste optimization settings ........................................................................... 79
8.3.2 Run automatic optimization .......................................................................................... 80
8.4 Optimize the iCRC gas flows........................................................................................... 82
8.5 Method optimization troubleshooting.......................................................................... 84
9Detector calibration.................................................................................................. 86
9.1 Attenuation modes......................................................................................................... 86
9.2 Instrument initialization and solution preparations..................................................... 88
9.3 Detector calibration worksheet...................................................................................... 89
9.3.1 Scan settings for attenuation calibration ...................................................................... 90
9.4 Detector calibration ........................................................................................................ 91
9.5 Attenuation factors on the existing worksheet............................................................. 94
9.6 Attenuation factors calibration troubleshooting .......................................................... 95
10 Maintenance and care.............................................................................................. 96
10.1 Maintenance intervals.................................................................................................... 97
10.2 Cleaning the exterior surfaces ....................................................................................... 98
10.3 Sample introduction system maintenance.................................................................... 99
10.3.1 Cleaning the nebulizer..................................................................................................100
10.3.2 Cleaning the spray chamber.........................................................................................102
10.3.3 Cleaning the transfer tube ...........................................................................................104
10.3.4 Cleaning the torch.........................................................................................................106
10.3.5 Replacing peristaltic pump tubing ...............................................................................107
10.4 Interface maintenance .................................................................................................108
10.4.1 Cleaning the sampler cone...........................................................................................108
10.4.2 Cleaning the skimmer cone..........................................................................................111
10.4.3 Cleaning the extraction lenses.....................................................................................113
10.5 Maintenance of the water cooler.................................................................................114
10.5.1 Cleaning the cooling fins ..............................................................................................114
10.5.2 Changing the cooling water .........................................................................................114
10.6 Maintenance of the fore-line roughing pump SV40BI ...............................................116
11 Autosampler ASPQ 3300 ....................................................................................... 119
11.1 Function and setup .......................................................................................................119
11.2 Installation ....................................................................................................................120

PlasmaQuant MS product family Contents
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11.3 Maintenance................................................................................................................. 123
11.3.1 Replacing the cannula and the sample tubing ...........................................................123
11.3.2 Replacing the pump tubing..........................................................................................125
11.3.3 Clean-up after cup overflow.........................................................................................125
11.3.4 Replacing the fuses....................................................................................................... 126
12 Troubleshooting .....................................................................................................127
13 Transport and storage............................................................................................ 129
13.1 Preparing the PlasmaQuant MS for transport............................................................129
13.2 Ambient conditions for transport and storage ...........................................................130
13.3 Recommissioning after transport or storage ..............................................................130
14 Disposal .................................................................................................................. 131
15 Specification ...........................................................................................................132
15.1 Technical data............................................................................................................... 132
15.1.1 ICP-MS data .................................................................................................................. 132
15.1.2 Control computer data .................................................................................................134
15.1.3 Water cooler data .........................................................................................................134
15.1.4 Autosampler ASPQ 3300 data.....................................................................................135
15.1.5 Autosampler Cetac ASX-560........................................................................................ 135
15.2 Guidelines and standards.............................................................................................136
16 Index.......................................................................................................................137

Figures PlasmaQuant MS product family
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Figures
Fig. 1 Safety markings on the PlasmaQuant MS ......................................................... 12
Fig. 2 PlasmaQuant MS – Front view with major components.................................. 21
Fig. 3 Sample introduction system in the PlasmaQuant MS ...................................... 22
Fig. 4 Concentric nebulizer (left) and spray chamber (right) ..................................... 23
Fig. 5 Standard one-piece torch ................................................................................... 23
Fig. 6 Torch installed in the plasma compartment ..................................................... 23
Fig. 7 Torch and induction coil...................................................................................... 24
Fig. 8 PlasmaQuant MS with unlatched interface door .............................................. 25
Fig. 9 Sampler cone installed in the PlasmaQuant MS ............................................... 26
Fig. 10 Skimmer cone installed in the PlasmaQuant MS .............................................. 26
Fig. 11 Relative size distinction between polyatomic and analyte ion ........................ 27
Fig. 12 ReflexION............................................................................................................. 28
Fig. 13 Curved pre-filters and mass analyzer................................................................. 29
Fig. 14 All-digital extended dynamic range detector.................................................... 29
Fig. 15 Setup of PlasmaQuant MS.................................................................................. 30
Fig. 16 Dimensions of the PlasmaQuant MS ................................................................. 38
Fig. 17 Connections and interfaces on the rear side of the device............................... 39
Fig. 18 Overview of connections on the right side of the instrument.......................... 40
Fig. 19 Switches and connections on the front side of the instrument........................ 40
Fig. 20 Transfer tube for Aerosol Dilution (left), standard transfer tube (right) ........ 47
Fig. 21 Sample tubing...................................................................................................... 50
Fig. 22 Peristaltic pump - tubing connections ............................................................... 51
Fig. 23 Pump tubing with Internal Standard tubing ..................................................... 52
Fig. 24 Peristaltic pump - tubing connections with Internal Standard ........................ 52
Fig. 25 Instrument summary window ............................................................................ 54
Fig. 26 Time scan displaying the signal during warm-up ............................................. 56
Fig. 27 Automatic torch alignment................................................................................. 60
Fig. 28 Peak resolution and trim .................................................................................... 61
Fig. 29 Mass calibration .................................................................................................. 62
Fig. 30 Detector voltage calibration ............................................................................... 63
Fig. 31 Setting up detector attenuation ......................................................................... 64
Fig. 32 Main page of the worksheet............................................................................... 66
Fig. 33 Selecting elements and isotopes........................................................................ 67
Fig. 34 Quickly adding elements and isotopes from the periodic table ....................... 68
Fig. 35 Selecting internal standard correction............................................................... 68
Fig. 36 Adding or modifying isotope correction equations........................................... 69
Fig. 37 Defining isotopes for the calculation of isotope ratios ..................................... 69
Fig. 38 Setting up calibration standards ........................................................................ 70
Fig. 39 Scan settings........................................................................................................ 72
Fig. 40 Sampling Settings................................................................................................ 73
Fig. 41 QC test settings.................................................................................................... 74
Fig. 42 Copying optimization settings ............................................................................ 77
Fig. 43 Default settings in the scan setup window........................................................ 78
Fig. 44 Selecting an optimization routine ...................................................................... 80
Fig. 45 Starting optimization .......................................................................................... 81
Fig. 46 Signals of analyte, interference and analyte/interference ratio...................... 82
Fig. 47 Schematic of detector attenuation..................................................................... 86
Fig. 48 Linear dynamic range of different attenuation modes..................................... 87
Fig. 49 Incorrect settings for detector calibration ......................................................... 90
Fig. 50 Configuring scan settings for attenuation calibration ...................................... 91
Fig. 51 Detector calibration tab ...................................................................................... 92

PlasmaQuant MS product family Figures
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Fig. 52 Detector attenuation correction factors viewer................................................. 92
Fig. 53 Graphical representation of attenuation factors ............................................... 93
Fig. 54 Saving attenuation correction factors ................................................................ 93
Fig. 55 Scan settings tab.................................................................................................. 94
Fig. 56 Selecting attenuation correction factors ............................................................ 94
Fig. 57 Nebulizer cleaning tool with installed nebulizer .............................................100
Fig. 58 Sampler cone in position with retaining ring and sampler cone tool ............108
Fig. 59 Skimmer cone in position and skimmer cone tool...........................................111
Fig. 60 Extraction lens 1, extraction lens 2 and retainer tool.....................................113
Fig. 61 Forevacuum pump.............................................................................................116
Fig. 62 Autosampler.......................................................................................................119
Fig. 63 Autosampler – overview....................................................................................120
Fig. 64 Connections on the right side of the autosampler ..........................................120
Fig. 65 Cannula with sample tube of the autosampler................................................124
Fig. 66 Autosampler cannula and sample tubing dismantled.....................................124
Fig. 67 Autosampler pump ............................................................................................125

Summaries PlasmaQuant MS product family
8
Summaries
Summary 1 Common elements and Interferences ...................................................... 27
Summary 2 Major components and their advantages................................................ 30
Summary 3 Environmental conditions for operation.................................................. 31
Summary 4 Power supply.............................................................................................. 32
Summary 5 Gas supply .................................................................................................. 33
Summary 6 Exhaust unit requirements........................................................................ 34
Summary 7 Cooling circuit requirements..................................................................... 34
Summary 8 Pump models ............................................................................................. 35
Summary 9 Fore-line roughing pump SV40BI............................................................. 35
Summary 10 Fore-line roughing pump XDS46i......................................................... 36
Summary 11 Information on the type plate............................................................... 41
Summary 12 Performance: PlasmaQuant MS, PlasmaQuant MS Q......................... 75
Summary 13 Performance: PlasmaQuant MS Elite S, PlasmaQuant MS Elite ........ 76
Summary 14 Detector attenuation with and without iCRC....................................... 87

PlasmaQuant MS product family Basic information
9
1Basic information
1.1 User manual notes
The user manual describes the following spectrometers of the PlasmaQuant series:
PlasmaQuant MS
PlasmaQuant MS Q
PlasmaQuant MS Elite S
PlasmaQuant MS Elite
In the text below these devices are collectively called PlasmaQuant MS. Differences in
the devices concerning the analytical performance are detailed in the section 8.
The user manual informs about the design and function of the PlasmaQuant MS and
provides the necessary know-how for the safe handling of the device and its
components. The user manual includes additional notes on the maintenance and
service of the equipment and potential causes and remedies of any faults.
Instructions for actions which occur in chronological order are numbered and
combined in action units.
Safety notes are indicated by pictographs and signal words. The type and source of the
danger are stated together with notes on preventing the danger.
The elements of ASpect MS software are indicated as follows:
Program terms are identified with SMALL CAPS (e.g., Menu FILE).
Buttons are shown by square brackets (e.g., [OK] button)
Menu items are separated by arrows (e.g., FILE OPEN).
The user manual uses the following symbols and signal words to indicate hazards or
instructions. The safety instructions are always placed before an action.
WARNING
Indicates a potentially hazardous situation which might cause fatal or very serious
injuries (deformities).
CAUTION
Indicates a potentially hazardous situation which might cause light or minor injuries.
NOTICE
Provides
indications of potential material and environmental damage.
Conventions
Symbols and signal words

Basic information PlasmaQuant MS product family
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1.2 Intended use
Inductively Coupled Plasma Mass Spectrometry (ICP-MS) is a powerful technique for
elemental and isotopic analysis that combines the efficiency and ease of use of the
inductively coupled plasma (ICP) with the sensitivity and selectivity of mass
spectrometry (MS).
The ICP mass spectrometer (ICP-MS) is typically used in an analytical laboratory for
the analysis of liquid – mainly aqueous – samples to determine the concentrations of
up to 75 elements from major to ultra-trace levels. Coupling of hyphenated
technologies like Laser Ablation for solid samples or liquid chromatography are
possible as well.
The PlasmaQuant MS may only be used for the measurements described in this user
manual. The license is limited to applications outside of in vitro diagnostics. The
operator is exclusively liable for damages as a result of improper use.
Special provisions have to be taken for the analysis of hydrofluoric acid and organics:
For hydrofluoric acid, we recommend the HF kit.
For organics we recommend the organics or volatile organics kit.
In addition, for organic solvents fire and health protection must be observed.

PlasmaQuant MS product family Safety instructions
11
2Safety instructions
2.1 General notes
For your own safety and to ensure error-free and safe operation of the
PlasmaQuant MS, please read this chapter carefully before using the instrument.
Observe all safety notes listed in this user manual and all messages and notes
displayed by the ASpect MS software.
The safety instructions given in the following chapters were taken from a risk
assessment. When handling the device there are certain residual risks which cannot be
further minimized by design measures. Some of these risks are subject to external
factors.
Besides the safety instructions in this user manual and the local safety regulations that
apply to the operation of the device, the general applicable regulations regarding
accident prevention, occupational health and safety and environmental protection
have to be observed and complied with.
References to potential dangers do not replace the work protection regulations which
must be observed.
2.2 Safety markings on the PlasmaQuant MS
Warnings and notice symbols have been attached to the PlasmaQuant MS which must
always be observed.
Damaged or missing warning and notice symbols can cause incorrect actions leading to
personal injury or hardware damage! The symbol labels must not be removed!
Damaged symbol labels must be replaced immediately!

Safety instructions PlasmaQuant MS product family
12
The following safety markings are attached to the PlasmaQuant MS:
On the rear side:
WARNING! Use only hydrogen and/or
helium.
Do NOT connect air, oxygen, or any
oxidizing gas or gas mix.
WARNING! Shock Hazard
High voltages inside
No user-serviceable parts under covers.
Contact your Analytik Jena
Representative for instrument repair and
service.
Warning of flammable
substances
(at hydrogen connection)
On the side wall:
To prevent damage to the skin and eyes
by UV radiation, NEVER look directly at
the plasma.
Fig. 1 Safety markings on the PlasmaQuant MS
2.3 Requirements for operating personnel
The PlasmaQuant MS must only be operated by qualified personnel instructed in the
use of the device. The instruction must also include conveying the content of this user
manual and the user manuals of other system components (for example, the fore-line
roughing pump).
In addition to the safety at work instructions in this user manual, the generally
applicable safety and accident prevention regulations of the respective country of
operation must be observed and adhered to. The operator must ascertain the latest
version of these regulations.
The user manual must be accessible to the operating and service personnel at all
times!
2.4 Safety instructions, transport and commissioning
Observe the following notes:
The PlasmaQuant MS is always installed by the service engineers of Analytik Jena
or an authorized and trained representative. Independent assembly and
installation is not permitted. Incorrect installation can create serious hazards.
The device weighs 186 kg. Use a lift truck to transport the device.
Four people are required to move the device in the laboratory by holding the device
on four firmly screwed-in carrying handles.

PlasmaQuant MS product family Safety instructions
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The device may only be placed on surfaces, such as desks, which were designed to
bear the specific load of the device.
The device must not be placed on an uneven or inclined surface to avoid tipping
over.
The instrument should never be moved or hit while the vacuum system is running.
2.5 Safety instructions - operation
2.5.1 General
Observe the following notes:
The operator of the PlasmaQuant MS must make sure before each commissioning
that the condition of the device, including its safety equipment, is sound.
Modifications, conversions and extensions to the device are only permitted after
consultation with Analytik Jena. Any unauthorized modifications can jeopardize the
device’s operational safety and may lead to limitations regarding the warranty and
access to customer service.
The device may only be operated if all protective equipment (e.g., interlocks and
covers) are in place, properly installed and fully operational. The sound condition of
the protection and safety equipment must be checked regularly. Any defects must
be corrected as soon as they occur. Protective and safety equipment must never be
removed, modified or decommissioned during operation.
During operation, free access to gas and water connections, the standby switch on
the front, the circuit breaker on the rear side of the device, and to the plasma off
button on the right side of the device, must always be ensured.
The ventilation equipment on the device must be in good working condition.
Covered vents or ventilation slits etc. may cause the device to break down or may
cause damage to it.
To insure the instrument´s safe and long-term use, never operate the plasma
unless both interface cones are installed. To prevent damage to the turbo pumps,
do not vent the vacuum system or open the gate valve unless both cones are
installed.
For organic solvents, explosion protection must be observed. If the spray chamber
drain is not pumped properly, the excess flammable solvents in the spray chamber
may ignite and become explosive. If the torch clogs, the increased pressure in the
sample introduction system may cause the spray chamber cap to blow off exposing
the solvent to ignition.
Caution when handing quartz glass and glass parts. Risk of broken glass and
therefore risk of injury!
Prevent any liquids, e. g. condensate, from entering the inside of the instrument.
The liquids might get into contact with electronic components and cause a short
circuit. Ensure uninterrupted air conditioning at high humidity, even during
operating pauses.
There is a risk of your hand being crushed in the peristaltic pump. Do not wear long
loose hair or loose-fitting clothes as they can get caught in the peristaltic pump.

Safety instructions PlasmaQuant MS product family
14
Danger of frostbite. The spray chamber and the inner parts of the thermostated
holder assembly may be very cold (≥ -15 °C). Do not touch these parts in normal
operation and directly after operation.
2.5.2 Safety instructions relating to ambient conditions
The PlasmaQuant MS must not be operated in explosion-hazard areas. Smoking or
open flames in the operating room of the PlasmaQuant MS are prohibited! Keep all
combustible materials away from the device.
2.5.3 Safety instructions - electrical equipment
Work on electrical components of the PlasmaQuant MS may only be performed by a
qualified electrical technician according to applicable electro-technical regulations.
Lethal voltages may occur in the device! Contact with live components may cause
death, serious injury or painful electrical shock.
Observe the following notes:
The mains plug must be connected to a proper CEE power socket to ensure that the
device meets protection class I (ground connector). It may only be connected to
power sources whose nominal voltage is the same as that specified on the type
plate of the equipment. The protective effect must not be invalidated by the use of
an extension line which does not have a protective conductor (→section "Power
supply" p. 32). Never use power cords with faulty or frayed insulation.
The following applies to the installation of the device in North America: When
using the power cable with a NRTL compliant connector, the type of connection
changes to "detachable cordset".
The PlasmaQuant MS and its system components must always be switched off
before being connected to the mains.
Any work on the electronics (within the device enclosure) may only be carried out
by the customer service of Analytik Jena or by a qualified technician authorized by
Analytik Jena.
Before opening the device and for any work on the electronics of the device, use
the circuit breaker on the rear panel to switch the device off. Disconnect the power
plug from the power socket.
2.5.4 Hazards caused by plasma operation
Plasma is extremely hot (> 6,000 K) and emits electromagnetic and UV radiation. The
induction coil is operated with 300 V RMS (and even higher at ignition) and 27 MHz.
High-frequency radiation and UV radiation can cause serious injuries to skin and eyes.
Contact with the torch (plasma torch) shortly after operation will cause skin burns. An
electrical discharge may also occur and cause fatal injuries, electrical shock and skin
injuries.
Shielding around the plasma compartment is designed to reduce UV, visible, and RF
radiation to safe levels while still permitting easy access to the torch when
maintenance is required.
Explosion protection

PlasmaQuant MS product family Safety instructions
15
The PlasmaQuant MS has an interlock system which is designed to extinguish the
plasma if either the plasma compartment lid or the interface door is opened. The
device will also extinguish the plasma when the flow rate of the cooling water or of the
argon gas and the extraction output drop below the required minimum.
Nevertheless, this device can potentially affect pacemaker function. If you have a
pacemaker or similar implanted device, consult your physician before using this device.
Observe the following notes:
Do not attempt to bypass the interlock system.
To ensure safe torch operation, you must not ignite the plasma unless the following
conditions are met:
The inner plasma compartment lid and interface door are closed and latched.
The torch is in working position.
Sufficient water flow is provided.
Argon flow is adequate.
The exhaust unit is switched on. The extraction output is within the range specified
in section "Exhaust unit" (p. 34).
The above mentioned components are backed up by several interlocks. Unless the
reliable functioning of these components is guaranteed, the plasma will not be ignited
or it will be extinguished automatically if a component reports a malfunction.
The viewing window and the UV shield incorporated into the instrument RF
enclosure enables you to view the plasma and operate the instrument without
being exposed to unsafe levels of UV radiation. Never violate the interlocks.
Before opening the plasma compartment lid or interface door, always extinguish
the plasma from the software.
There are two ports for sample introduction on the front and on the right-side of
the instrument. Do not look through these openings directly into the plasma.
Allow the torch, cones, coils, interface, and torch compartment to cool before
carrying out any work in this area, or wear heat-resistant gloves.
2.5.5 Behavior in case of ring plasmas
In the following situations, press the orange plasma off button (on the right side of the
instrument) immediately to prevent the torch from melting:
The plasma produces loud noises (crackles).
The shape of the plasma changes considerably and a bright ring is visible on the
inside of the coil.
Torch parts begin to glow.
In the pre-ignition phase, a light pink discharge is normal.

Safety instructions PlasmaQuant MS product family
16
2.5.6 Safety instructions relating to the formation of ozone and toxic vapors
The interaction between the UV radiation from the plasma and the surrounding air
results in the formation of a high concentration of toxic gases such as ozone and
nitrogen oxides.
Observe the following notes:
The PlasmaQuant MS may only be operated with an active exhaust unit.
The exhaust unit must be switched on before igniting the plasma.
2.5.7 Safety instructions relating to compressed gas containers and systems
The principal gas used by the PlasmaQuant MS is argon. The only gases to be used in
the integrated Collision Reaction Cell (iCRC) are hydrogen and helium. The
PlasmaQuant MS is also fitted with N2or O2connections for the Nitrox option. Do not
connect any other gases than those specified to the correct inlet fittings.
Observe the following notes:
Gases are taken from compressed gas cylinders or local liquid gas systems. The
required purity of the gases must be ensured.
Work on compressed gas cylinders and systems must only be carried out by
individuals with specialist knowledge and experience in compressed gas systems.
For compressed gas operation, the safety instructions and guidelines which are
valid at the operating location must be strictly complied with.
Cylinders must be used and stored only in a vertical position and secured to an
immovable structure or a properly constructed cylinder stand. Move cylinders only
by securing them to a properly constructed cart.
High pressure hoses and pressure reducers may only be used for the assigned
gases.
All pipes, hoses and screw connections must be checked weekly for leaks and
externally visible damage. Possible pressure losses from closed systems and lines
under pressure must be determined. Leaks and damages must be repaired
immediately.
Any leak (except that of air or oxygen) can result in an oxygen-deficient
atmosphere which can cause asphyxiation. The area in which cylinders are stored
and the area surrounding the instrument must be adequately ventilated to prevent
such gas accumulations.
The gas supply must be closed prior to servicing and repairs.
After successful repair and servicing of components of the compressed gas
containers or system, the device must be checked for sound operation prior to
recommissioning.
PlasmaQuant MS instruments may be used with hydrogen gas in the iCRC system.
Appropriate safety precautions must be taken, as Hydrogen is combustible over a wide
range of concentrations. A hydrogen fire may be invisible under bright ambient light.
The temperature increases with gas expansion, and if it is allowed to expand rapidly
from high pressure, hydrogen gas can self-ignite.
Hydrogen Safety

PlasmaQuant MS product family Safety instructions
17
Observe the following notes:
Instead of using pressurized gas cylinders as a source of hydrogen, consider using a
hydrogen gas generator. These devices only produce the required amount of
hydrogen. There is no storage of hydrogen gas when the system is not being used.
In the case of an accident hydrogen gas may escape into the laboratory
atmosphere. Always ensure the exhaust unit is active when hydrogen gas is used.
In the event of a power failure, hydrogen gas may continue to flow from the supply
even if the PlasmaQuant MS is completely off. Always manually shut off the
hydrogen gas supply valve when there is no power to the PlasmaQuant MS. In
general, shut off the hydrogen gas supply when you are not using the iCRC.
Oxygen gas is a powerful oxidizer which will rapidly accelerate combustion of many
materials. Oxygen cylinders should be handled with care because serious explosions
can result from contact between oil and high pressure oxygen.
Observe the following notes:
Oil or grease should never be used on connections to oxygen cylinder or gas line
carrying oxygen.
Oxygen is incompatible with all flammable materials and should be stored
separately.
2.5.8 Handling of samples, auxiliary and operating materials
Observe the following notes:
The operator is responsible for the selection of substances used in the process as
well as for their safe handling. This is particularly important for radioactive,
infectious, poisonous, corrosive, combustible, explosive and otherwise dangerous
substances.
When handling dangerous substances, local safety codes and guidelines must be
observed.
Warnings on labels must always be observed. Only use clearly marked containers as
well as protective goggles and rubber gloves. Use suitable body protection (coat,
safety glasses and rubber gloves) when handling samples. Ensure sufficient
ventilation.
Cleaning with hydrofluoric acid must be carried out in a fume hood. When handling
hydrofluoric acid, rubber aprons, gloves and face masks must be worn.
Biological samples must be handled according to local guidelines regarding the
handling of infectious material.
When measuring materials containing cyanide, make sure prussic acid cannot be
generated in the waste container, i.e., the waste solution must not be acidic.
Ensure that all residue liquid from the nebulizer and the autosampler is directed
into a waste container.
The operator is responsible for ensuring that waste materials such as residue
liquid from the waste container are disposed of in an environmentally responsible
manner and according to local regulations.
Oxygen Safety

Safety instructions PlasmaQuant MS product family
18
Examples of dangerous organic solvents:
Methyl isobutyl ketone (MIBK) Flammable, highly volatile, noxious-smelling
Toluene Flammable, hazardous to health
Kerosene Flammable, hazardous to the aquatic environment,
hazardous to health
Methanol, ethanol, propanol Flammable, partly acutely toxic
Tetrahydrofuran (THF) Flammable, hazardous to health, extremely volatile,
dissolves polyethylene and polystyrene
This list is incomplete insofar, that other solvents could be considered for use with the
ICP-MS. In cases of uncertainty about an unknown liquid, this may only be used when
the manufacturer has confirmed that there is no danger to safety.
2.5.9 Safety instructions relating to cleaning and decontamination measures
Observe the following notes:
The operator is responsible for carrying out suitable decontamination should the
device be contaminated externally or internally with dangerous substances.
Spots, drops or larger spillages should be removed and cleaned up using water and
an absorbent material such as cotton wool, laboratory wipes or cellulose. The
affected areas must then be wiped with a disinfectant (e. g. Incidin Plus solution).
Before using a cleaning or decontamination procedure other than that prescribed,
check that the intended procedure will not damage the device.
2.6 Behavior during emergencies
Observe the following notes:
During an emergency, if there is no immediate danger of injury, you must
extinguish the plasma immediately by using the plasma off button. This is the
orange button on the right side of the instrument.
If possible, switch off the device at the circuit breaker at the rear of the instrument
after allowing a cooling down period of 30 s. Then disconnect the mains plugs of
the device and the system components from their power sockets.
Close the gas supply as soon as possible after switching off the devices.

PlasmaQuant MS product family Safety instructions
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2.7 Safety instructions - maintenance and repair
Observe the following notes:
The PlasmaQuant MS is always serviced by the service engineers of Analytik Jena or
an authorized and trained representative. Otherwise the warranty is void. The
operator may only carry out the tasks listed in the chapter "Maintenance and care".
The exterior of the PlasmaQuant MS may only be cleaned with a damp cloth. Use
only water and, if required, commercial surfactant. Do not use organic solvents or
abrasive cleaners.
Carefully close the interface door and the plasma compartment lid after
maintenance. There is a risk of your hand being crushed.
If water or other liquids are found to leak out of the instrument, contact the service
engineers.
Risk of damage to health due to improper decontamination! Perform a professional
and documented decontamination of the device before returning it to Analytik
Jena. The decontamination protocol is provided by the service when the return is
registered. Analytik Jena must refuse acceptance of contaminated devices. The
sender may be liable for any damage caused by inadequate decontamination of the
device.
Use only original spare parts, wear parts and consumables. They have been tested
and ensure safe operation. Glass parts are wear parts and are not subject to the
warranty.

Function and setup of the PlasmaQuant MS PlasmaQuant MS product family
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3Function and setup of the PlasmaQuant MS
3.1 Physical principle of ICP-MS
Inductively coupled plasma mass spectrometry (ICP-MS) is a powerful technique for
elemental and isotopic analysis that combines the efficiency and ease of use of the
inductively coupled plasma (ICP) with the sensitivity and selectivity of mass
spectrometry (MS).
The inductively coupled plasma (ICP) used in analytical spectrometry is an
atmospheric-pressure plasma, generated in argon gas flowing through a specially
designed torch. Energy transfers into the plasma by inductive coupling of the plasma
electrons and ions within the magnetic field of a radio-frequency coil.
Inductively coupled plasma offers an advantage over chemical flames with its much
higher temperature. Samples introduced into the ICP heat to more than 6 000 K. This
high temperature is focused on a very small area of approx. 5 cm3. The sample is
introduced to the plasma in the form of aerosol (small droplets in a gas). The droplets
typically decompose, vaporize, atomize and ionize very quickly and effectively.
The inductively coupled plasma has been used commercially as an atomization and
excitation source for optical emission spectrometry for over 30 years. Many of the
most intense lines in ICP optical emission spectra are produced by singly-charged ions;
therefore the ICP makes an efficient ion source for mass spectrometry. In ICP nearly all
the metals are over 90 % ionized. Even elements with relatively high ionization
potentials (such as P, As, Hg and I) are over 20 % ionized.
The ICP ion source operates at atmospheric pressure (ca. 1 bar), while the mass
spectrometer operates at a very low pressure (ca. 7 x 10-5 mbar). Efficient transfer of
ions from the ICP to the mass spectrometer is therefore a major challenge,
complicated by the fact that the plasma is at very high temperatures and by the high
power radio frequency currents present in the plasma.
In the PlasmaQuant MS an ionized sample is extracted from the plasma through two
interface cones into the mass spectrometer. The pressure is reduced gradually in three
stages by differential pumping. The PlasmaQuant MS uses the ReflexION, a 3D
focusing, 90-degree ion mirror, to focus ions into the mass analyzer. The ion optics
allows elimination of neutrals, photons, droplets and solids that would otherwise
produce high background signals and would contaminate the mass analyzer.
The quadrupole mass spectrometer separates ions of a specific mass to charge ratio. In
the PlasmaQuant MS patented self-cleaning curved fringe rods are mounted in front of
the mass analyzer. These pre-filters cause the ions to follow a curved trajectory
shielding the quadrupole from excited neutrals. The all-digital (AD) Detector, a
Discrete Dynode Electron Multiplier with eleven orders of dynamic range, records ions
after they exit the mass spectrometer.