Endress+Hauser KIO1 Technical specifications

Products Solutions Services
Brief Operating Instructions
Accessory optics for the Rxn-10
probe
KIO1, KNCO1, KLBIO1, KRSU1,
KRBMO, KRBSL
KA01551C/66/EN/03.22
70201227

Brief Operating Instructions Accessory optics for the Rxn-10 probe
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Accessory optics for the Rxn-10 probe Brief Operating Instructions
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Table of Contents
1About this document..................................................................................................5
1.1 Disclaimer ........................................................................................................................................................................5
1.2 Warnings .........................................................................................................................................................................5
1.3 U.S. export compliance....................................................................................................................................................5
2Basic safety instructions............................................................................................6
2.1 Requirements for the personnel ....................................................................................................................................6
2.2 Designated use ................................................................................................................................................................6
2.3 Workplace safety.............................................................................................................................................................7
2.4 Operational safety...........................................................................................................................................................7
2.5 Service safety ...................................................................................................................................................................8
2.6 Important safeguards......................................................................................................................................................8
2.7 Product safety..................................................................................................................................................................8
3Product description ....................................................................................................9
3.1 Immersion optic (KIO1) ..................................................................................................................................................9
3.2 Non-contact optic (KNCO1)..........................................................................................................................................10
3.3 bIO-Optic (KLBIO1) .......................................................................................................................................................10
3.4 Bio multi optic (KRBMO) and bio sleeve (KRBSL) ......................................................................................................11
3.5 Raman optic system for single use (KRSU1) ...............................................................................................................13
4Incoming product acceptance and product identification ......................................14
4.1 Incoming acceptance.....................................................................................................................................................14
4.2 Product identification ...................................................................................................................................................14
4.3 Scope of delivery............................................................................................................................................................14
5Installation ...............................................................................................................15
5.1 Installing immersion optics and bIO-Optics................................................................................................................15
5.2 Installing the bio multi optic and bio sleeve system...................................................................................................16
5.3 Installing non-contact optics........................................................................................................................................22
5.4 Installing the Raman optic system for single use .......................................................................................................23
6Commissioning.........................................................................................................27
6.1 Receipt of optics ............................................................................................................................................................27
6.2 Calibration and verification..........................................................................................................................................27
7Operation..................................................................................................................29
7.1 bIO-Optic, bio multi optic and bio sleeve system, and Raman optic system for single use .....................................29
7.2 Storing the bio multi optic and the resusable portion of the Raman optic system for single use ..........................29
8Diagnostics and troubleshooting.............................................................................31

Brief Operating Instructions Accessory optics for the Rxn-10 probe
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1About this document
1.1 Disclaimer
These instructions are Brief Operating Instructions; they do not replace the Operating
Instructions included in the scope of supply.
1.2 Warnings
Structure of Information
Meaning
Causes (/consequences)
Consequences of non-compliance (if
applicable)
‣ Corrective action
This symbol alerts you to a dangerous situation.
Failure to avoid the dangerous situation can result in a
fatal or serious injury.
Causes (/consequences)
Consequences of non-compliance (if
applicable)
‣ Corrective action
This symbol alerts you to a dangerous situation.
Failure to avoid this situation can result in minor or
more serious injuries.
Cause/situation
Consequences of non-compliance (if
applicable)
‣ Action/note
This symbol alerts you to situations which may result
in damage to property.
Table 1. Warnings
1.3 U.S. export compliance
The policy of Endress+Hauser is in strict compliance with U.S. export control laws as
detailed in the website of the Bureau of Industry and Security at the U.S. Department of
Commerce.

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2Basic safety instructions
The safety information in this section is specific to the optics that are compatible with the
Rxn-10 Raman spectroscopic probe. Refer to the Rxn-10 Raman spectroscopic probe
Operating Instructions for additional information related to probe and laser safety.
2.1 Requirements for the personnel
•Installation, commissioning, operation, and maintenance of the probe/optics may be
carried out only by specially trained technical personnel.
•Technical personnel must be authorized by the plant operator to carry out the specified
activities.
•Technical personnel must have read and understood these Operating Instructions and
must follow the instructions contained herein.
•The facility must designate a laser safety officer who ensures staff are trained on all
Class 3B laser operating and safety procedures.
•Faults at the measuring point may only be rectified by authorized trained personnel.
Repairs not described in this document must be carried out only at the manufacturer's
site or by the service organization.
2.2 Designated use
The Rxn-10 Raman spectroscopic probe is designed for product and process development as
well as manufacturing (when used with the Raman optic system for single use). The probe is
compatible with a wide range of interchangeable, commercially available optics (immersion
and non-contact) to meet the requirements of different applications.
Recommended applications for the optics include:
Optic
Fields of application
Immersion optic (IO)
▪Development laboratory
▪Pharmaceutical: drug substance unit operations, reaction
analysis, crystallization, end-point detection, solvent swaps
▪Chemical: material identification, reaction analysis,
polymerization, cross-linking, blending
▪Food and beverage: blending, purification, natural and synthetic
components
Non-contact optic
(NCO)
▪Polymer solids (pellets, films, or powders)
▪Pharmaceutical drug product manufacturing
▪Raw material identification
▪Meat or fish quality
▪Formulation optimization

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Optic
Fields of application
bIO-Optic
▪Benchtop bioreactors to measure glucose, lactate, amino acids,
cell density, titer, and more
▪Benchtop fermenters to measure glycerol, methanol, ethanol,
sorbitol, biomass, and more
▪Use with Flow assembly CYA680 for select downstream
bioprocessing applications
Bio multi optic and
bio sleeve
▪Benchtop bioreactors to measure glucose, lactate, amino acids,
cell density, titer, and more
▪Benchtop fermenters to measure glycerol, methanol, ethanol,
sorbitol, biomass, and more
▪Use with Flow assembly CYA680 for select downstream
bioprocessing applications
Raman optic system
for single use
▪Single-use bioreactors to measure glucose, lactate, amino acids,
cell density, titer, and more
▪Single-use fermenters to measure glycerol, methanol, ethanol,
sorbitol, biomass, and more
Table 2. Fields of application
The above table lists common applications for the Rxn-10 probe and optics. There are other
possible fields of application; however, use of the device for any purpose well outside of the
fields of application described here poses a threat to the safety of people and of the entire
measuring system and invalidates any warranty.
2.3 Workplace safety
As the user, you are responsible for complying with the following safety conditions:
•Installation guidelines
•Local standards and regulations for electromagnetic compatibility
2.4 Operational safety
Before commissioning the entire measuring point:
•Verify that all connections are correct.
•Ensure that electro-optical cables are undamaged.
•Ensure fluid level is sufficient for probe/optics immersion (if applicable).
•Do not operate damaged products, and protect them against unintentional operation.
•Label damaged products as defective.

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During operation:
•If faults cannot be rectified, products must be taken out of service and protected against
unintentional operation.
•When working with laser devices, always follow all local laser safety protocols which
may include the use of personal protective equipment and limiting device access to
authorized users.
Service ratings may include limitations for fittings, flanges, or seals. Installer must
understand these limitations and use appropriate hardware and assembly procedures for a
pressure-tight and safe joint.
2.5 Service safety
Follow your company’s safety instructions when removing a process probe/optic from the
process interface for service. Always wear proper protective equipment when servicing the
equipment.
2.6 Important safeguards
•Do not use the optics for anything other than their intended use.
•Do not look directly into the laser beam.
•Do not point the laser at a mirrored/shiny surface or a surface that may cause diffuse
reflections. The reflected beam is as harmful as the direct beam.
•When not in use, close the shutter on the Rxn-10 probe. If an optic cap is available,
place it on the unused optic.
•Always use a laser beam block to avoid inadvertent scatter of laser radiation.
2.7 Product safety
The product is designed to meet all current safety requirements, has been tested, and
shipped from the factory in a safe operating condition. The relevant regulations and
international standards have been observed. Devices connected to an analyzer must comply
with the applicable analyzer safety standards.

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3Product description
The variety of optics available for the Rxn-10 Raman spectroscopic probe, powered by Kaiser
Raman technology, enables flexible sampling options in the laboratory, process
development , or single use manufacturing environment. Endress+Hauser offers immersion,
bioprocess, and non-contact optics for the analysis of liquids, slurries and solids. Optics are
available in multiple lengths and sizes to meet the requirements of different applications.
Refer to the sections below for descriptions of the optics types and their use.
•3.1: Immersion optic
•3.2: Non-contact optic
•3.3: bIO-Optic
•3.4: Bio multi optic and bio sleeve
•3.5: Raman optic system for single use
3.1 Immersion optic (KIO1)
The Endress+Hauser immersion optic is suited for use with the Rxn-10 probe in reaction
vessels, laboratory reactors, or process streams. It has a fixed focus design and no movable
parts, providing long-term measurement stability and superior signal performance. The
sealed design is the standard for use with embedded Raman Rxn analyzers.
Figure 1. Immersion optics with varying shaft length and diameter
Figure 2. Tips of immersion optics with varying diameters
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3.2 Non-contact optic (KNCO1)
Paired with the Rxn-10 probe, the Endress+Hauser non-contact optic provides contact-free
Raman measurements of samples either directly or through sight glass or translucent
packaging. These optics are ideal for use with solids or turbid media or when sample
contamination or damage to optical components is a concern.
Figure 3. Non-contact optics in varying sizes
3.3 bIO-Optic (KLBIO1)
The Endress+Hauser bIO-Optic is a versatile immersion optic used in conjunction with the
Rxn-10 probe. It measures multiple, specific bioprocessing components in real-time and is
compatible with standard PG13.5 bioreactor ports. The fixed focus design of the bIO-Optic
provides long-term measurement stability along with superior signal performance, essential
for transferable, high performance Raman-based bioprocess analysis. Available in various
industry standard lengths, the bIO-Optic is ideally suited for benchtop bioreactor/fermentor
applications requiring headplate entry. The 120 mm version is compatible with
Endress+Hauser flow assembly CYA680.
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Figure 4. bIO-Optics in varying lengths
The bIO-Optic should NOT be used with hydrocarbon solvents (including ketones and
aromatics).
These solvents can degrade probe performance and invalidate the warranty.
3.4 Bio multi optic (KRBMO) and bio sleeve (KRBSL)
The Endress+Hauser bio multi optic and bio sleeve make up a versatile two part immersion
optic system used in conjunction with the Rxn-10 probe. This system measures multiple,
specific bioprocessing components in real-time and is compatible with standard PG13.5
bioreactor ports.
The system is comprised of the following parts:
•A reusable bioprocessing multi optic, which has no product contact, and
•The bioprocessing sleeve that interfaces with the bio multi optic and has product
contact. The bio sleeve has a lifespan of 10 autoclave cycles when used in conjunction
with the bio sleeve desiccator.
The modular design of this sample system allows for calibration of the optic without
removing the bio sleeve from the bioreactor/wetted area. Another benefit realized from the
modular design is reduced probe service and maintenance because of the simplification of
the wetted/sterilized portion. The fixed focus design provides long-term measurement
stability and superior signal performance, essential for transferable, high performance
Raman-based bioprocess analysis.
The bio multi optic and bio sleeve are available in 120 mm and 220 mm (4.73 in. and
8.67 in.) industry standard lengths. The 120 mm version is compatible with
Endress+Hauser flow assembly CYA680. The sample system is ideally suited for benchtop
bioreactor/fermentor applications requiring headplate entry.
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Figure 5. Bio multi optic and bio sleeve system in varying lengths
This system should NOT be used with hydrocarbon solvents (including ketones and
aromatics).
These solvents can degrade probe performance and invalidate the warranty.
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3.5 Raman optic system for single use (KRSU1)
The Endress+Hauser Raman optic system for single use was developed according to industry
standards for single use sensors and is designed for single use bioprocess applications. The
system is used in conjunction with the Rxn-10 probe and is comprised of the following
parts:
•The reusable optic, which has no product contact, and
•A disposable fitting, which is installed, tested, and supplied ready to use from the single
use vessel vendor.
Figure 6. Reusable optic (top) and disposable fitting (bottom)
The Raman optic system for single use should NOT be used with hydrocarbon solvents
(including ketones and aromatics).
These solvents can degrade probe performance and invalidate the warranty.
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4Incoming product acceptance and product
identification
4.1 Incoming acceptance
•Verify that the packaging is undamaged. Notify the supplier of any damage to the
packaging. Keep the damaged packaging until the issue has been resolved.
•Verify that the contents are undamaged. Notify the supplier of any damage to the
delivery contents. Keep the damaged goods until the issue has been resolved.
•Check that the delivery is complete and nothing is missing. Compare the shipping
documents with your order.
•Pack the product for storage and transportation in such a way that it is protected
against impact and moisture. The original packaging offers the best protection. Make
sure to comply with the permitted ambient conditions found in the Accessory optics for
the Rxn-10 probe Operating Instructions manual.
If you have any questions, please contact your supplier or your local sales center.
Incorrect transportation can damage the optics.
4.2 Product identification
4.2.1 Label
At a minimum, the optics are labeled with the following information:
•Manufacturer information
•Serial number
Compare the information on the label/tag with the order.
4.2.2 Manufacturer address
Endress+Hauser
371 Parkland Plaza
Ann Arbor, MI 48103 USA
4.3 Scope of delivery
The scope of delivery comprises:
•Selected optic(s)
•Accessory optics for the Rxn-10 probe Operating Instructions manual
If you have any questions, please contact your supplier or local sales center.

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5Installation
The installation information in this section is specific to the optics that are compatible with
the Rxn-10 Raman spectroscopic probe. Refer to the Rxn-10 Raman spectroscopic probe
Operating Instructions for additional information related to probe installation.
The Rxn-10 probe is compatible with both immersion optics and non-contact optics. The
probe has a torque limiting clamp that secures the immersion optics. The clamp also holds
the adapter for non-contact optics.
Before installation, ensure any protective covers are removed from the optics.
When replacing an optic in a probe, use the multi optic calibration accessory or the Raman
Calibration Accessory (HCA) to perform an intensity calibration for that probe with the new
optic. Refer to the Accessory optics for the Rxn-10 probe Operating Instructions to determine
the appropriate calibration method for each optic.
5.1 Installing immersion optics and bIO-Optics
Endress+Hauser immersion optics and bIO-Optics slip into the Rxn-10 probe and are
secured by a torque limiting, thumb screw-based clamp. The thumb screw on the Rxn-10
probe should never be fully removed.
When installing or removing immersion optics, ensure the laser and emission shutter
are in the closed position.
To install an immersion optic:
1. If necessary, loosen the metal thumb screw on the Rxn-10 probe by turning the screw
counter-clockwise approximately one turn (do not remove). Then find the probe end of
the optic, which is the end that includes the product markings.
2. Insert the probe end of the optic through the end optic clamp.
3. Push the optic back until it stops.
4. Tighten the thumb screw by gently turning it clockwise until there is an audible “click”
sound. This indicates the thumb screw has reached the desired torque. Failure to
tighten the screw will result in the optic coming loose, potentially damaging the optic.
5. After installing an optic into a probe, use the Raman Calibration Accessory to perform
an intensity calibration for the probe with the new optic.

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Figure 7. Installing an immersion optic (IO) into the Rxn-10 probe
To remove an immersion optic:
Loosen the torque limiting thumb screw by turning it counter-clockwise approximately one
turn so that the immersion optic is released from its clamp. Do not remove the screw. Then
slide the immersion optic out.
5.2 Installing the bio multi optic and bio sleeve system
Installation of the bio multi optic and bio sleeve system into a bioreactor for data acquisition
is comprised of the following steps:
•Installing the bio multi optic into the Rxn-10 probe
•Installing the bio sleeve into the bioreactor
•Sterilizing the bio sleeve/bioreactor combination (typically)
•Installing the Rxn-10 probe with bio multi optic into the bio sleeve/bioreactor
5.2.1 Installing the bio multi optic into the Rxn-10 probe
The Endress+Hauser bio multi optic slips into the Rxn-10 probe and is secured by a torque
limiting, thumb screw-based clamp. The thumb screw on the Rxn-10 probe should never be
fully removed.
When installing or removing optics, ensure the laser and emission shutter are in the
closed position.
To install the optic into the probe:
1. If necessary, loosen the metal thumb screw on the Rxn-10 probe by turning the screw
counter-clockwise approximately one turn (do not remove).
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2.
3.
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2. Insert the optic through the end optic clamp.
Figure 8. Inserting the bio multi optic into the Rxn-10 probe
3. Push the optic back until it stops.
Figure 9. Final position of bio multi optic in the Rxn-10 probe
4. Tighten the thumb screw by gently turning it clockwise until there is an audible “click”
sound. This indicates the thumb screw has reached the desired torque. Failure to
tighten the screw will result in the optic coming loose, potentially damaging the optic.
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Figure 10.Tightening the thumb screw on the Rxn-10 probe
5. After installing an optic into a probe, use the multi optic calibration accessory to
perform an intensity calibration for the probe with the new optic. Alternately, the
Raman Calibration Accessory (HCA) may be used, but a bio sleeve is required.
To remove the bio multi optic from the Rxn-10 probe:
Loosen the torque limiting thumb screw by turning it counter-clockwise approximately one
turn so that the optic is released from its clamp. Do not remove the screw. Then slide the
optic out.
5.2.2 Installing the bio sleeve into the bioreactor
The bio sleeve is designed to interface with a PG13.5 threaded port on the bioreactor. To
install the bio sleeve into the bioreactor:
1. Align the bio sleeve with the available PG13.5 port.
2. Insert the bio sleeve into the PG13.5 port on the bioreactor.
3. Tighten the PG13.5 nut into the PG13.5 port of bioreactor according to the bioreactor
manufacturer’s guidelines.
It is critical that there is no interference between the bio sleeve and the internal
stirring device.
Interference can damage the optic system and may cause cavitation.
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Figure 11.Installing the bio sleeve into the PG13.5 port of the bioreactor
The bioreactor/bio sleeve will typically need to be sterilized prior to use.
The desiccator must be installed into the bio sleeve prior to autoclave sterilization.
Refer to the Accessory optics for the Rxn-10 probe Operating Instructions for autoclaving
instructions.
5.2.3 Installing the Rxn-10 probe with bio multi optic into the bio sleeve
Prior to insertion in the bio sleeve, the Rxn-10 probe with the reusable optic should be
calibrated and verified.
See Section 6.2 → for calibration and verification instructions.
After the Rxn-10 with bio multi optic is calibrated and the bioreactor with installed bio
sleeve is sterilized, the systems need to be joined to take data. Follow the steps below.
1.
2.
3.
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1. Obtain the sanitary flange seal.
If the sanitary flange seal is already present on the bio sleeve, go to step 3.
Figure 12.Sanitary flange seal
2. Place the sanitary flange seal onto the seal gland.
Figure 13.Installing the sanitary flange seal
3. Confirm that the bio multi optic for use is the correct immersion length.
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