Endress+Hauser Analytik Jena Biometra TRIO Series User manual

Operating manual
BiometraTRIO
Triple Powered PCR thermal cycler

Biometra GmbH (Analytik Jena AG)
Rudolf-Wissell-Str. 30
37079 Göttingen Germany
Phone + 49 551 50 68 6-0
Fax + 49 551 50 68 6-66
Email [e-mail]
Biometra GmbH
Rudolf-Wissell-Str. 30
37079 Göttingen Germany
Phone + 49 551 50 68 6 -12, -14 or -16
Fax + 49 551 50 68 6-66
Email [e-mail]
http://www.analytik-jena.com
Operating manual valid from software version ME 1.70 –RE 1.46
Microsoft and Windows are registered trademarks of Microsoft Corp.
The identification with ® or TM is omitted in this manual.
34-9990-113-23-e
03_19
Analytik Jena AG
© Copyright 2019 Analytik Jena AG
Manufacturer
Service
General Information
Copyrights and Trade-
marks
Documentation number
Edition
Technical documentation

Biometra TRIO Manual Contents
Version 03_19 3
Contents
1Basic Information............................................................................................................. 9
1.1 User manual notes ...................................................................................................... 9
1.2 Intended use ............................................................................................................. 10
1.3 Warranty and liability................................................................................................ 10
2Safety instructions ......................................................................................................... 11
2.1 General notes............................................................................................................ 11
2.2 Safety markings on the Biometra TRIO..................................................................... 11
2.3 Technical condition ................................................................................................... 11
2.4 Requirements for the operating personnel ............................................................... 12
2.5 Safety instructions: transport and installation .......................................................... 12
2.6 Safety instructions –operation ................................................................................. 12
2.6.1 General...................................................................................................................... 12
2.6.2 Safety instructions: protection against explosion and fire ........................................ 13
2.6.3 Safety instructions: electrical equipment .................................................................. 13
2.6.4 Handling of samples, auxiliary and operating materials ........................................... 14
2.7 Safety instructions –maintenance and repair........................................................... 14
2.8 Behavior during emergencies.................................................................................... 14
3Function and setup ........................................................................................................ 15
3.1 Field of Applications.................................................................................................. 15
3.2 General Description................................................................................................... 15
3.3 Special features......................................................................................................... 16
3.3.1 Thermal Block ........................................................................................................... 16
3.3.2 High Performance Smart Lid..................................................................................... 16
3.3.3 Display ...................................................................................................................... 16
3.3.4 User-specific quick start function .............................................................................. 17
3.3.5 Easy programming .................................................................................................... 17
3.3.6 Temperature Optimisation Step (TOS)...................................................................... 17
3.3.7 GLP compliance......................................................................................................... 18
3.3.8 Open system philosophy ........................................................................................... 18
3.4 Order numbers .......................................................................................................... 18
4Installation and commissioning ................................................................................... 19
4.1 Content of delivery.................................................................................................... 19
4.2 Unpack and Check ..................................................................................................... 19
4.3 Installation Conditions............................................................................................... 19
4.4 Operation Voltage ..................................................................................................... 20
5Control elements............................................................................................................ 21
5.1 The Biometra TRIO front view................................................................................... 21
5.2 The Biometra TRIO rear view.................................................................................... 21
5.3 High Performance Smart Lid (HPSL)......................................................................... 22
5.4 Loading samples ....................................................................................................... 22
5.5 The Biometra TRIO Software .................................................................................... 23
5.5.1 Keyboard................................................................................................................... 23
5.5.2 Keypad ...................................................................................................................... 24
5.5.3 Cursor keys................................................................................................................ 25
5.5.4 Confirm or Cancel...................................................................................................... 25
5.5.5 List of used commands and icons.............................................................................. 25
6Operating........................................................................................................................ 30
6.1 Power On Self-Test ................................................................................................... 30
6.2 Log in Screen............................................................................................................. 30
6.3 Log in ........................................................................................................................ 31
6.4 Home Screen ............................................................................................................. 32
6.5 Block status ............................................................................................................... 33
6.6 Quick start function ................................................................................................... 34

Contents Biometra TRIO Manual
4 Version 03_19
6.7 Incubation Mode ....................................................................................................... 36
7Creating, editing and saving programs ........................................................................ 39
7.1 Programming modes................................................................................................. 39
7.2 Create a new program/Use program template ......................................................... 40
7.3 Assign program names ............................................................................................. 42
7.4 Set heated lid temperature ....................................................................................... 42
7.5 Set preheating mode................................................................................................. 43
7.6 Edit parameter or step .............................................................................................. 44
7.6.1 Set cycles................................................................................................................... 45
7.6.2 Set temperature increment....................................................................................... 46
7.6.3 Set time increment.................................................................................................... 46
7.6.4 Adjust ramp rate ....................................................................................................... 47
7.6.5 Set Temperature Optimisation Step (TOS)................................................................ 47
7.7 Insert step ................................................................................................................. 49
7.8 Delete step ................................................................................................................ 49
7.9 Save program............................................................................................................ 49
8Starting, Copying and Deleting programs....................................................................52
8.1 Start program............................................................................................................ 52
8.2 Copy program............................................................................................................ 53
8.3 Copy all programs ..................................................................................................... 55
8.4 Delete program ......................................................................................................... 56
8.5 Delete all programs................................................................................................... 56
9Running, pausing, continuing and stopping programs...............................................58
9.1 Display during run..................................................................................................... 58
9.2 Pause Program.......................................................................................................... 59
9.3 Continue Program..................................................................................................... 60
9.4 Skip Step ................................................................................................................... 60
9.5 Stop Program ............................................................................................................ 61
10 Protocol Wizard ..............................................................................................................62
10.1 Select Polymerase ..................................................................................................... 62
10.2 Enter New Polymerase or edit Polymerase ............................................................... 62
10.3 Delete Polymerase .................................................................................................... 64
10.4 Protocol Wizard parameter screen............................................................................ 65
10.5 Primer Annealing Temperature Calculator................................................................ 66
11 Tools................................................................................................................................ 70
11.1 Settings..................................................................................................................... 70
11.1.1 Set Date and Time..................................................................................................... 71
11.1.2 Automatic user logout............................................................................................... 72
11.1.3 Configure Beeper ...................................................................................................... 73
11.1.4 Network .................................................................................................................... 74
11.1.5 Display brightness..................................................................................................... 76
11.1.6 Touch screen calibration ........................................................................................... 76
11.1.7 Factory settings......................................................................................................... 77
11.2 User Management .................................................................................................... 78
11.2.1 Create user................................................................................................................ 80
11.2.2 Edit user.................................................................................................................... 81
11.2.3 Delete user ................................................................................................................ 84
11.3 Documentation ......................................................................................................... 84
11.3.1 Run-Logfile ............................................................................................................... 85
11.3.2 Power-On-Logfile ..................................................................................................... 87
11.3.3 Extended Self-Test-Logfile........................................................................................ 88
11.3.4 Error-Logfile.............................................................................................................. 89
11.4 Extended Self-Test.................................................................................................... 89
11.5 Service Info File (SINF).............................................................................................. 92
11.6 Backup ...................................................................................................................... 92
11.7 Cycler Info ................................................................................................................. 93

Biometra TRIO Manual Contents
Version 03_19 5
11.8 Contact...................................................................................................................... 94
12 Adaptation of programs ................................................................................................ 95
13 Short Manual.................................................................................................................. 96
14 Fault removal ................................................................................................................. 96
14.1 Forgotten password ................................................................................................ 100
14.2 Slow heating and cooling ........................................................................................ 100
14.3 Auto restart............................................................................................................. 100
14.4 Auto restart due to unrecognized power failure...................................................... 100
14.5 Mirror programs to several thermal cyclers ............................................................ 101
14.6 Adapt protocols from other cyclers.......................................................................... 101
14.7 Release wheel in case of blocked lid........................................................................ 101
15 Maintenance and Repair .............................................................................................102
15.1 Factory calibration................................................................................................... 102
15.2 Servicing and Repair................................................................................................ 102
15.3 Cleaning .................................................................................................................. 102
15.4 Disinfecting the device ............................................................................................ 103
15.5 Firmware update..................................................................................................... 103
15.6 Spare parts .............................................................................................................. 103
16 Service...........................................................................................................................104
16.1 Instructions for returnshipment.............................................................................. 104
16.2 Packing of the Biometra TRIO Thermal Cycler......................................................... 105
17 Disposal.........................................................................................................................106
18 Technical specifications...............................................................................................107
19 Declaration of Conformity...........................................................................................109
20 Index .............................................................................................................................110

Contents Biometra TRIO Manual
6 Version 03_19
Figures
Figure 1 Biometra TRIO Thermal Cycler ..................................................................... 15
Figure 2 Biometra TRIO front view ............................................................................. 21
Figure 3 Biometra TRIO rear view............................................................................... 21
Figure 4 Biometra TRIO lid open button .................................................................... 22
Figure 5 Biometra TRIO keyboard............................................................................... 23
Figure 6 Biometra TRIO keypad .................................................................................. 24
Figure 7 Biometra TRIO start screen........................................................................... 30
Figure 8 Biometra TRIO log in screen ......................................................................... 31
Figure 9 Biometra TRIO select user screen................................................................. 31
Figure 10 Biometra TRIO enter password screen..................................................... 32
Figure 11 Biometra TRIO home screen..................................................................... 32
Figure 12 Biometra TRIO block status/quick start button....................................... 33
Figure 13 Biometra TRIO quick start screen............................................................. 35
Figure 14 Biometra TRIO multiblock selection query .............................................. 36
Figure 15 Biometra TRIO incubation mode screen .................................................. 37
Figure 16 Biometra TRIO spreadsheet programming.............................................. 39
Figure 17 Biometra TRIO graphical programming screen....................................... 39
Figure 18 Biometra TRIO program overview screen................................................ 41
Figure 19 Biometra TRIO program template “Blank”............................................... 41
Figure 20 Biometra TRIO program template screen................................................ 42
Figure 21 Biometra TRIO lid temperature setting screen........................................ 43
Figure 22 Biometra TRIO edit step screen ................................................................ 45
Figure 23 PCR program with loop ............................................................................. 46
Figure 24 Biometra TRIO Temperature Optimisation Step screen ......................... 48
Figure 25 Program overview screen.......................................................................... 50
Figure 26 Quick selection screen ............................................................................... 51
Figure 27 Biometra TRIO program overview screen................................................ 52
Figure 28 Biometra TRIO program overview screen with program preview.......... 53
Figure 29 Biometra TRIO multiblock selection query .............................................. 53
Figure 30 Biometra TRIO program overview screen................................................ 54
Figure 31 Biometra TRIO program overview screen................................................ 55
Figure 32 Biometra TRIO security query................................................................... 56
Figure 33 Biometra TRIO security query................................................................... 57
Figure 34 Biometra TRIO run screen in graphical view ........................................... 58
Figure 35 Biometra TRIO run screen in spreadsheet view. ..................................... 59
Figure 36 Biometra TRIO run screen during program pause................................... 60
Figure 37 Biometra TRIO query for multiblock selection......................................... 61
Figure 38 Biometra TRIO security query................................................................... 61
Figure 39 Biometra TRIO polymerase selection screen ........................................... 62
Figure 40 Biometra TRIO screen for editing program templates for polymerases 63
Figure 41 Biometra TRIO for saving program templates for polymerases............. 64
Figure 42 Biometra TRIO security query................................................................... 64
Figure 43 Biometra TRIO protocol wizard parameter screen.................................. 65
Figure 44 Biometra TRIO protocol wizard view parameter screen ......................... 66
Figure 45 Biometra TRIO security query................................................................... 66
Figure 46 Biometra TRIO primer annealing temperature calculator screen .......... 67
Figure 47 Biometra TRIO screen for primer concentration adjustment ................. 68
Figure 48 Biometra TRIO tools screen ...................................................................... 70
Figure 49 Biometra TRIO settings screen ................................................................. 71
Figure 50 Biometra TRIO date and time screen ....................................................... 72
Figure 51 Biometra TRIO automatic user logout screen.......................................... 73

Biometra TRIO Manual Contents
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Figure 52 Biometra TRIO beeper configuration screen............................................ 74
Figure 53 Network settings screen, IP settings via static......................................... 75
Figure 54 Network settings screen, IP settings via DHCP ........................................ 75
Figure 55 Biometra TRIO display brightness settings screen .................................. 76
Figure 56 Biometra TRIO touch screen calibration screen....................................... 77
Figure 57 Biometra TRIO security query ................................................................... 77
Figure 58 Biometra TRIO user management screen ................................................ 78
Figure 59 Biometra TRIO configure user management screen ............................... 79
Figure 60 Biometra TRIO create user screen ............................................................ 81
Figure 61 Biometra TRIO user data screen ............................................................... 82
Figure 62 Biometra TRIO user properties screen...................................................... 83
Figure 63 Biometra TRIO security query ................................................................... 84
Figure 64 Biometra TRIO documentation screen ..................................................... 85
Figure 65 Biometra TRIO Run-Logfile overview screen ........................................... 85
Figure 66 Biometra TRIO Run-Logfile screen ........................................................... 86
Figure 67 Biometra TRIO View program screen ....................................................... 87
Figure 68 Biometra TRIO Power-On-Logfile screen................................................. 88
Figure 69 Biometra TRIO Extended Self-Test-Logfile screen.................................. 88
Figure 70 Biometra TRIO Error-Logfile screen ......................................................... 89
Figure 71 How to load plastic ware for Extended Self-Test..................................... 90
Figure 72 Biometra TRIO Extended Self-Test screen............................................... 91
Figure 73 Biometra TRIO Extended Self-Test progress overview screen................ 91
Figure 74 Biometra TRIO create Service Info File screen ......................................... 92
Figure 75 Biometra TRIO backup file screen............................................................. 93
Figure 76 Biometra TRIO cycler info screen.............................................................. 94
Figure 77 Biometra TRIO contact details screen ...................................................... 94

Contents Biometra TRIO Manual
8 Version 03_19

Biometra TRIO Basic Information
Version 03_19 9
1Basic Information
1.1 User manual notes
The Biometra TRIO is intended for operation by qualified specialist personnel observ-
ing this operating manual.
For the Biometra TRIO, there are 3 different models available. In the text below these
devices are collectively called Biometra TRIO. Differences are explained in the corre-
sponding sections.
The operating manual contains information about the design and operation of the
Biometra TRIO and provides personnel familiar with PCR technology with the neces-
sary know-how for the safe handling of the equipment and its components. The oper-
ating manual further includes notes on the maintenance and service of the device and
potential causes and remedies of any faults.
These operating instructions apply to all Biometra TRIO as of software version ME
1.70 - RE 1.46 (see "Cycler Info" p. 93).
Furthermore, sometimes the terms protocol and program are used synonymously,
these are one or more programmed temperature steps with different or the same
holding times and cycles.
Instructions for actions which occur in chronological order are numbered and com-
bined in action units.
Safety instructions are indicated by pictographs and signal words. The type and source
of the danger are stated together with notes on preventing the danger.
The elements of the control and analysis program are indicated as follows:
Program terms are identified with SMALL CAPS (e.g., Menu FILE).
Buttons are shown by square brackets (e.g., [OK] button)
Menu items are separated by arrows (e.g., FILE OPEN).
The operating manual uses the following symbols and signal words to indicate hazards
or instructions. The safety instructions are always placed before an action.
WARNING
Indicates a potentially hazardous situation which might cause fatal or very serious
injuries (deformities).
CAUTION
Indicates a potentially hazardous situation which might cause light or minor injuries.
NOTICE
Provides indications of potential material and environmental damage.
Conventions
Symbols and signal words

Basic Information Biometra TRIO
10 Version 03_19
Symbol for waste disposal in accordance with WEEE directive:
Do not dispose of in household waste
CE marking
China RoHS marking
1.2 Intended use
The Biometra TRIO is an end-point thermal cycler. It is designed to amplify nucleic
acids by repeated cycles of heating and cooling using DNA polymerases in a PCR reac-
tion.
The thermal cycler is developed for Research Use Only (RUO).
The Biometra TRIO combines modern design with user-friendly software. The user
interface consists of a touchscreen with a graphical or spreadsheet display that shows
the time, the status and the temperature program for each run. Using the touchscreen
keyboard, information and program parameters can be entered directly on the screen.
Due to the high ramp rates, the Biometra TRIO thermal cycler is suitable for fast PCR
applications, thereby helping to shorten program run times.
1.3 Warranty and liability
The warranty duration and liability comply with the legal requirements and the provi-
sions in the general terms and conditions of Analytik Jena AG.
Deviations from the intended use described in this manual result in limitations of war-
ranty and liability in the event of damage.
Warranty and liability claims are excluded for personal injury and property damage
due to one or several of the following causes:
use of the Biometra TRIO other than intended
improper commissioning, operation and servicing of the device
modifications of the Biometra TRIO without prior consultation with Analytik Je-
na/Biometra GmbH
unauthorized intervention in the Biometra TRIO
operation of the Biometra TRIO with faulty safety equipment
use of other than original spare parts, wearing parts or consumables
improper repairs
improper transport and storage of the Biometra TRIO
faults due to the non-observance of this manual

Biometra TRIO Safety instructions
Version 03_19 11
2Safety instructions
2.1 General notes
For your own safety and to ensure error-free and safe operation of the Biometra TRIO,
please read this section carefully before commissioning.
Besides the safety instructions in this user manual and the local safety regulations that
apply to the operation of the device, the general applicable regulations regarding acci-
dent prevention, occupational health and safety and environmental protection have to
be observed and complied with.
References to potential dangers do not replace the work protection regulations which
must be observed.
2.2 Safety markings on the Biometra TRIO
Damaged or missing safety symbols can cause incorrect actions leading to personal
injury or material damage. Do not remove safety symbols! Replace damaged safety
symbols immediately!
The following safety symbols are attached to the inside of the heated lid and the rear
of the device:
CAUTION! Hazard area!
Proceed with due care when handling samples and working with the Biometra TRIO.
CAUTION! Risk of burns!
There is a risk of burning at the heated lid, the thermal block, the samples, and the
rear of the device.
The following sign is attached to the underside of the Biometra TRIO:
ATTENTION! Keep ventilation slits clear!
Keep the ventilation slits on the underside and at the rear of the device clear. Make
sure that there are no objects underneath the device (e.g., paper) that may be sucked
against the ventilation slot and thus interfere with ventilation.
2.3 Technical condition
The Biometra TRIO has been built and certified according to safety standard
EN 61010-1. Do not modify the device in any way. Any modification of the device will
lead to a loss of warranty and the EN 61010-1 certificate and poses a potential risk.

Safety instructions Biometra TRIO
12 Version 03_19
The following has to be observed:
The operator must only operate the device in a sound and operationally safe condi-
tion. The technical condition must always comply with the legal requirements and
regulations.
Prior to every use the device must be checked for damage and sound condition.
Any changes in the device affecting its safety must be reported by the operating
personnel to the operator without delay.
2.4 Requirements for the operating personnel
The Biometra TRIO must only be operated by qualified specialist personnel instructed
in the use of the device. The instructions also include imparting the contents of this
manual.
In addition to the safety at work instructions in this operating manual the generally
applicable safety and accident prevention regulations of the respective country of op-
eration must be observed and adhered to. The operator must ascertain the latest ver-
sion of these regulations.
The operating manual must be accessible to the operating and service personnel at all
times.
2.5 Safety instructions: transport and installation
The following has to be observed:
Drain the sample block before transporting the Biometra TRIO. Make sure there are
no sample tubes in the block.
Only ship the Biometra TRIO in its original packaging.
Please verify that the delivery is complete on receipt of the Biometra TRIO and
check for possible transport damage. In case of claims please contact Biometra
GmbH.
2.6 Safety instructions –operation
2.6.1 General
The operator of the Biometra TRIO must make sure before each commissioning that
the condition of the device including the safety equipment is sound.
The following has to be observed:
Free access to the power switch on the back of the enclosure has to be ensured
during operation.
The ventilation fittings at the rear of the device must be unobstructed and opera-
tional. Covered ventilation grilles or slits etc. may cause the device to break down or
may cause damage to it.

Biometra TRIO Safety instructions
Version 03_19 13
The use of oil between the samples and the sample block is not necessary to
achieve an improved heat exchange. However, if you still want to use oil, you
should use mineral oil. Do not use silicone oil.
Be careful to avoid crushing or pinching injuries when closing the device.
The Biometra TRIO may cause burns. The following has to be observed:
The thermal block, the samples and the heated lid reach high temperatures. There
is a risk of burns during contact.
Ensure that the lid is securely closed before starting the program! Do not touch the
heated lid!
The rapid heating of the thermal block can cause liquids to boil explosively. Always
wear safety goggles during operation!
Do not touch hot sample tubes or plates and do not open them or boiling liquid
may escape!
Only use plates and tubes that are suitable for high temperatures (up to 100 °C), fit
well into the thermal block (no shaking) and whose lids seal tightly!
2.6.2 Safety instructions: protection against explosion and fire
The Biometra TRIO must not be operated in an explosive environment. The Biometra
TRIO must not be operated with flammable, explosive or volatile substances.
2.6.3 Safety instructions: electrical equipment
Work on the electrical components of the Biometra TRIO may only be performed by a
qualified electrical technician in accordance with the applicable electrical engineering
rules. Life-threatening electrical voltages may occur in the interior of the Biometra
TRIO!
The following has to be observed:
Any work on the interior of the device may only be carried out by the customer
service of Biometra GmbH and specially authorized technicians.
The electrical components must be checked regularly by a qualified electrician. Any
defects, such as loose connections, faulty or damaged cables, must be repaired
without delay.
Before opening the device it must be switched off at the power switch and the
mains plug must be disconnected from the mains outlet!
The Biometra TRIO must be switched off immediately using the power switch (on
the equipment back plate) and the mains plug disconnected from the power supply
if any faults occur in the electric components.
Do not operate the device near sources of strong electromagnetic radiation (for
example, an unshielded, deliberately operated high frequency source), as these
may affect proper operation of the device.

Safety instructions Biometra TRIO
14 Version 03_19
2.6.4 Handling of samples, auxiliary and operating materials
The operator is responsible for the selection of substances used in the process as well
as for their safe handling. This is particularly important for radioactive, pathogenic,
infectious, poisonous, corrosive or otherwise dangerous substances. For details contact
the safety officer responsible for your location. When handling dangerous substances
local safety codes and guidelines must be observed. The following general notes do
not replace the specific local regulations or the regulations in the safety data sheets of
the manufacturers for the auxiliary and operating materials.
The following has to be observed:
Protective goggles and rubber gloves have to be worn when handing reagents.
If only a few samples are treated, an (empty) tube of the same height must addi-
tionally be placed at each corner position of the block. If the number of samples in
the block is too low, there is a risk of the tubes being damaged and sample liquid
leaking out.
For your own safety, please observe the potential infectious qualities of the exam-
ined biological material.
2.7 Safety instructions –maintenance and repair
The Biometra TRIO is usually repaired by the service department of Biometra GmbH or
its authorized and trained specialist personnel. Unauthorized repairs can damage the
device. Therefore, the operator may generally only carry out the tasks listed in section
"Maintenance and Repair" p. 102.
The following has to be observed:
The exterior of the device may only be cleaned with a damp, not dripping, cloth
after the device has been switched off.
Do not use alcohol (e.g., methanol or ethanol), organic solvents or abrasives to
clean the device.
Any maintenance on the device may usually only be carried out in the switched-off
condition (unless stated otherwise).
2.8 Behavior during emergencies
In case of danger or accidents, immediately switch off the Biometra TRIO using the
main switch on the equipment back plate. Disconnect the mains plug from the power
supply!

Biometra TRIO Function and setup
Version 03_19 15
3Function and setup
3.1 Field of Applications
Developed in 1983 by Kary Mullis PCR (Polymerase Chain Reaction) is now a common
and often indispensable technique used in medical and biological research labs for a
variety of applications. The Biometra TRIO is an end-point Thermal Cycler and intend-
ed to amplify nucleic acids by repeated cycles of heating and cooling using DNA-
Polymerases in a PCR reaction. The development of the instrument had the goal to
combine latest technology with stylish design and user-friendly software. The user
interface consists of a touchscreen with a graphical or tabular display that shows the
time, status, and temperature profile for each run. A touchscreen keypad allows you to
enter information into fields on the screen. The Biometra TRIO can run three inde-
pendent PCR reactions simultaneously and is especially helpful when versatile samples
are to be incubated using different PCR programs.
3.2 General Description
There are three models of the Biometra TRIO Thermal Cycler:
Biometra TRIO 48 for 0.2 ml tubes, plates or strips
Biometra TRIO 30 for 0.5 ml tubes
Biometra TRIO combi for 0.2 ml tubes, plates or strips or 0.5 ml tubes
The instrument is controlled by a 7’’ touchscreen display and easy to use interface. PCR
protocols and Run-Logfiles can be saved to a connected USB drive. By using the USB
functions PCR protocols can be exchanged easily between instruments and Run-
Logfiles can be stored for documentation reasons on PC.
Figure 1 Biometra TRIO Thermal Cycler

Function and setup Biometra TRIO
16 Version 03_19
The Biometra TRIO Thermal Cycler features an automatic restart. If a power failure
occurs during the run the instrument will continue the run when the power returns. In
case of long- term power failure (longer than 30 minutes) the instrument keeps the
sample block at 4°C(freeze step) and the user can decide to repeat the run with the
same samples or to discard them.
This instruction manual provides information on how to use all models of the Biometra
TRIO Thermal Cycler most effectively.
3.3 Special features
3.3.1 Thermal Block
The Biometra TRIO Thermal Cycler is available with different block formats. The 48
well version can be used with standard 0.2 ml tubes, strips or plates. With 30 well
blocks the Biometra TRIO can be used with 0.5 ml tubes and the combi block version
offers the flexibility to incubate 0.2 ml tubes, strips or plates or 0.5 ml tubes. With a
sample capacity of up to 144 samples in parallel the Biometra TRIO 48 can also be
used for high throughput applications.
By the rubber sealing at the lid an encapsulated space is formed when the lid is closed.
The closed room serves to improve the temperature uniformity of the sample block
and avoids formation of condensed water at the final PCR cooling step. Furthermore
all blocks are perfectly sealed to prevent condensed water from penetrating the Peltier
elements underneath the sample block and other parts of the electronics. By the per-
fect sealing the Peltier elements are protected and the instruments life time is ex-
panded.
3.3.2 High Performance Smart Lid
The Biometra TRIO heated lid has been optimized to heat twice as fast as with older
models and thus helps to shorten protocol run times. Moreover the heated lid serves
for two additional essential functions: It prevents the formation of condensed water at
the reaction tube portion located above the block surface level and it ensures reliable
contact between samples and thermal block by applying constant pressure. By the
integrated clutch mechanism the pressure applied to the reaction tubes is always same
regardless of the plastic ware height. The combination of the heated lid shape and the
reliable contact pressure ensure an even temperature distribution between samples
thus significantly improves the temperature uniformity.
With one press on the front button, the lid gently swings open and arrests in its end
position. By this mechanism the lid cannot fall down and the heated lid is out of the
radius of action when the user is inserting tubes or plates. Thus the risk of injury by
violent pressure or the risk of burn by hot surfaces is prevented.
3.3.3 Display
The Biometra TRIO thermal cycler features a 7" state-of-the-art color touchscreen user
interface. The touchscreen is built-in at a flat angle to ensure reflection-free viewing
and ergonomic programming. For programming PCR protocols the software incorpo-
rates Biometra's proven spreadsheet philosophy and alternatively offers graphical
programming. One touch of a button leads from the spreadsheet to the alternative

Biometra TRIO Function and setup
Version 03_19 17
graphical programming mode. This makes the creation of new programs or editing
existing programs fast and easy.
3.3.4 User-specific quick start function
Many other thermal cyclers offer a so-called "List of latest programs used". In the list of
latest programs used a certain number of PCR programs is collected and offered for
quick start. The list is typically ordered only by date and the user has to search for the
desired program. In contrast the Biometra TRIO software creates a user-specific list
and only the latest programs started by the currently logged-in user are offered for
quick start. Even if a lab member does not use the device for a longer time, the infor-
mation on the latest programs started by this user will not get lost.
For maximum convenience the Biometra TRIO software additionally features a pro-
gram preview. Before the start of a program the protocol steps are summarized in a
clearly arranged table by the program preview tool. The preview therefore provides a
comprehensive overview of the protocol structure without the need to access pro-
gramming screens. The program preview is also available before the quick start of
programs.
3.3.5 Easy programming
Creating new PCR programs takes much time if for each single step all parameters are
set manually. The Biometra TRIO offers two options for easy protocol creation:
1. Pre-installed program templates
2. Protocol wizard
The Biometra TRIO software offers several pre-installed program templates for differ-
ent applications. The program templates provide a general protocol structure and can
be easily adapted for the current experiment.
The protocol wizard offers the comfort to create specific PCR programs by just a few
inputs. The protocol wizard is based on program templates for specific polymerases.
For up to eight different polymerases program templates can be saved in the Biometra
TRIO software and just the annealing temperature, cycle number and product length
has to be filled in to create a specific PCR program. Optionally two-step or three-step
PCR programs can be created by the software. Furthermore the protocol wizard in-
cludes a primer annealing temperature calculator and the calculated Ta value can be
used for protocolcreation.
3.3.6 Temperature Optimisation Step (TOS)
Finding the best primer annealing temperature is crucial for the specificity and effi-
ciency of PCR reactions. Often optimization of experiments is conducted only limited
leading to unspecific byproducts or reduced PCR sensitivity. By using the Biometra
TRIO Temperature Optimisation Step (TOS) function new primer pairs with unknown
annealing temperatures can be tested quickly and optimized in very short time.
The Temperature Optimisation Step (TOS) function makes use of the three sample
blocks and provides three different temperatures for a selected program step. Just set
the temperature for sample block 2 and define the temperature difference (tempera-
ture increment) for block 1 and 3. Since the Biometra TRIO samples blocks work inde-
pendent of each other the maximum programmable temperature difference between
the sample blocks is at ±47.5 °C.

Function and setup Biometra TRIO
18 Version 03_19
3.3.7 GLP compliance
The software can manage up to 90 user accounts. By default three different user-
levels are available: Administrator, user with generic rights and user with limited
rights. However, in a comfortable menu the administrator can configure the settings
for each user individually by activating or inactivating single rights. By the user admin-
istration tool in combination with the password protection of user accounts access to
the instrument can be limited to authorized persons and unwanted modifications to
the system settings and PCR protocols prevented.
With each system start the Biometra TRIO performs an initial self-test and additionally
the software offers an extended self-test that can be initiated by the user. The results
of the extended self-test becomes are summarized in a protocol and are stored by the
instrument. Additionally to the self-test functions the instruments creates and stores
run-logfiles for each single run. For long-term storage run-logfiles and extended self-
test protocols can be exported as proprietary file format.
3.3.8 Open system philosophy
The Biometra TRIO is an open system regarding consumables and reagents. Depend-
ing upon the sample block the system can be used with single tubes, strips or plates.
PCR plates can be standard profile or low profile and they can be skirted, semi-skirted
or non-skirted. The Biometra TRIO can handle all kind of plates and the plastic ware be
sealed with domed caps, flat caps, sealing foil or other technologies. Regardless of the
used sealing method thanks to the HPSL technology (see chapter 3.3.2) always the
same pressure is applied to the consumables serving for absolutely reproducible condi-
tions.
The Biometra TRIO Thermal Cycler is not limited to reagents from a specific vendor
and can be used with any kind of polymerase. Regardless if isothermal-, hot start- or
non-hot start polymerases are used, the Biometra TRIO will produce reliable results.
E.g. by programming a pause step for the initial denaturation the system can be used
for manual hot start applications. After inserting samples into the hot block the PCR
protocol can be started just by pressing “continue”.
3.4 Order numbers
Model
Block type
Order No.
Biometra TRIO 30
Aluminum
846-x-070-720
Biometra TRIO 48
Aluminum
846-x-070-723
Biometra TRIO combi
Aluminum
846-x-070-724
Abbrevations:
x = 2 for 230 V, 4 for 115 V, 5 for 100 V

Biometra TRIO Installation and commissioning
Version 03_19 19
4Installation and commissioning
4.1 Content of delivery
Thermal Cycler
Mains connector
Manual
Short Manual
Please keep the original packaging material for return shipment in case of servicing.
The Biometra TRIO Thermal Cycler shipping box provides a specially developed system
with form blocks for a safe transport of this electronic device.
4.2 Unpack and Check
Unpack and carefully examine the instrument. Report any damage to Biometra.
WARNING
Do not attempt to operate this device if physical damage is present!
Please keep the original packing material for return shipment in case of service issues.
4.3 Installation Conditions
Place the Biometra TRIO Thermal Cycler on a stable surface in a dry, safe environ-
ment. For details see working conditions in table "Technical specifications" (see
chapter 18 p. 107 ).
Let equilibrate the Biometra TRIO Thermal Cycler to room temperature before
starting operation (1 to 6 h).
Make sure that the appliance connector and the plug of the supply cord are acces-
sible, so you can separate the instrument from the mains.
Make sure that the ventilation slots on the bottom and the rear are not obstructed
(see Figure 3 p. 21). Make sure that there is no object underneath the Thermal Cy-
cler that may block the ventilation slots at the bottom (e.g. a piece of paper etc.)
There must be sufficient distance between the ventilation slots on the rear side of
the Thermal Cycler and a wall or another instrument (min 10 cm).
In case of several cyclers side by side running simultaneously we would recommend
to keep a distance of at least 10 cm between every thermal cycler.
Ensure that both the rear and bottom ventilation slits of the rear and bottom of the
instrument are unobstructed. Insufficient ventilation can cause overheating of the
instrument.

Installation and commissioning Biometra TRIO
20 Version 03_19
Make sure that the main supply voltage is in accordance with the label above the
power connection (see chapter "Operation Voltage" p. 20).
Connect the Biometra TRIO Thermal Cycler to a grounded socket.
Prior to connecting the unit to the power source, ensure that the voltage selector at
the back side of the instrument is set to the required voltage.
WARNING
Danger of electric shock! Unplug the power cable before you open the Biometra TRIO
Thermal Cycler!
The display contrast can be adjusted to local light conditions.
4.4 Operation Voltage
WARNING
Danger of electric shock! Prior to connecting the Biometra TRIO Thermal Cycler to the
mains, make sure that the setting of the voltage selector is in accordance with your
mains voltage.
The Biometra TRIO Thermal Cycler can operate at 100, 115 or 230 Volt. The operation
Voltage is shown on the Voltage selector, which is located at the instrument bottom.
To change operation voltage, switch off the instrument and disconnect the mains plug.
Use a coin or another round shape item to turn the adjustment slot of the Voltage
selector to the new Voltage.