GE Amersham Imager 600 User manual

Amersham™Imager 600
Including:
600, 600 UV, 600 QC, 600 RGB
Operating Instructions
Original instructions

Table of Contents
41 Introduction ..........................................................................................................
51.1 Important user information .............................................................................................................
71.2 Regulatory information ......................................................................................................................
112 Safety instructions ...............................................................................................
122.1 Safety precautions ...............................................................................................................................
222.2 Labels .........................................................................................................................................................
252.3 Emergency procedure ........................................................................................................................
262.4 Recycling information .........................................................................................................................
272.5 Declaration of Hazardous Substances (DoHS) ........................................................................
293 Instrument description .......................................................................................
303.1 Introduction .............................................................................................................................................
313.2 Feature overview ..................................................................................................................................
323.3 Illustration of the instrument ...........................................................................................................
333.4 Instrument hardware description .................................................................................................
403.5 Instrument software description ...................................................................................................
413.5.1 Description of the Capture tab .................................................................................................
463.5.2 Description of the Library tab ...................................................................................................
483.5.3 Description of the image view ..................................................................................................
523.5.4 Description of the analysis workflow ....................................................................................
553.5.5 Description of settings views ....................................................................................................
593.6 Accessories ..............................................................................................................................................
624 Installation ............................................................................................................
634.1 Site requirements ..................................................................................................................................
654.2 Instrument setup ..................................................................................................................................
664.3 System settings .....................................................................................................................................
674.3.1 Date and time ..................................................................................................................................
694.3.2 Focus ...................................................................................................................................................
714.3.3 Instrument indicator .....................................................................................................................
724.3.4 Service ................................................................................................................................................
744.4 Network .....................................................................................................................................................
754.4.1 Network setup .................................................................................................................................
794.4.2 Network access from instrument ............................................................................................
814.4.3 Instrument access from network ............................................................................................
845 Operation ..............................................................................................................
855.1 Operation flow chart ...........................................................................................................................
865.2 Turn on the instrument ......................................................................................................................
895.3 Select tray ................................................................................................................................................
905.4 Place the tray in the instrument ....................................................................................................
935.5 Capture an image .................................................................................................................................
945.5.1 Available methods .........................................................................................................................
955.5.2 Chemiluminescence capture .....................................................................................................
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Table of Contents

1185.5.3 Colorimetric capture .....................................................................................................................
1225.5.4 Fluorescence capture ...................................................................................................................
1445.6 View the image ......................................................................................................................................
1485.7 Save and manage image files ........................................................................................................
1495.7.1 Save locations .................................................................................................................................
1525.7.2 Save images .....................................................................................................................................
1575.7.3 File manage options .....................................................................................................................
1605.8 Analyze the image ................................................................................................................................
1635.8.1 Lane creation ...................................................................................................................................
1655.8.2 Background Subtraction .............................................................................................................
1695.8.3 Band detection ................................................................................................................................
1715.8.4 Molecular weight assignment ..................................................................................................
1745.8.5 Normalization ..................................................................................................................................
1755.8.6 Summary ...........................................................................................................................................
1775.9 Turn off the instrument ......................................................................................................................
1786 Analysis software .................................................................................................
1796.1 Software installation and activation ............................................................................................
1806.1.1 Amersham Imager 600 Analysis Software installation .................................................
1836.1.2 Amersham Imager 600 Analysis Software registration ................................................
1876.2 Software operation ..............................................................................................................................
1916.2.1 Lane creation ...................................................................................................................................
1936.2.2 Background subtraction .............................................................................................................
1986.2.3 Band detection ................................................................................................................................
2006.2.4 Molecular weight assignment ..................................................................................................
2036.2.5 Normalization ..................................................................................................................................
2046.2.6 Summary ...........................................................................................................................................
2077 Maintenance .........................................................................................................
2087.1 Instrument ...............................................................................................................................................
2097.2 Accessories ..............................................................................................................................................
2117.3 Regular inspections .............................................................................................................................
2128 Troubleshooting ...................................................................................................
2138.1 General problems .................................................................................................................................
2158.2 Problems with image quality ...........................................................................................................
2178.3 Problems with the software .............................................................................................................
2209 Specifications ........................................................................................................
224Index .......................................................................................................................
Amersham Imager 600 Operating Instructions 29-0645-17 AD 3
Table of Contents

1 Introduction
Purpose of this document
The Operating Instructions provide you with the instructions needed to operate, and
maintain Amersham Imager 600 in a safe way.
Prerequisites
In order to operate Amersham Imager 600 in the intended way, the following prerequisites
must be fulfilled:
•You have read and understood the safety instructions in this Operating Instruction
•You should be acquainted with the use of general laboratory equipment and with
handling of biological materials
•The instrument is installed by GE representatives
About this chapter
This chapter contains important user information, description of safety notices, regula-
tory information, and a general description of the intended use of Amersham Imager
600.
In this chapter
See pageSection
51.1 Important user information
71.2 Regulatory information
4 Amersham Imager 600 Operating Instructions 29-0645-17 AD
1 Introduction

1.1 Important user information
Read this before operating
Amersham Imager 600
All users must read the entire Operating Instructions before installing, operating or
maintaining Amersham Imager 600.
Always keep the Operating Instructions at hand when operating Amersham Imager 600.
Do not operate Amersham Imager 600 in any other way than described in the user
documentation. If you do, you may be exposed to hazards that can lead to personal injury
and you may cause damage to the equipment.
Intended use
Amersham Imager 600 is a CCD camera system that produces digitial images of samples
in gels or membranes in three different modes: chemiluminescence, colorimetric, and
fluorescence.
Amersham Imager 600 is intended for research use only, and shall not be used in clinical
procedures, or for diagnostic purposes.
Safety notices
This user documentation contains safety notices (WARNING, CAUTION, and NOTICE)
concerning the safe use of the product. See definitions below.
WARNING
WARNING indicates a hazardous situation which, if not avoided,
could result in death or serious injury. It is important not to proceed
until all stated conditions are met and clearly understood.
Amersham Imager 600 Operating Instructions 29-0645-17 AD 5
1 Introduction
1.1 Important user information

CAUTION
CAUTION indicates a hazardous situation which, if not avoided,
could result in minor or moderate injury. It is important not to pro-
ceed until all stated conditions are met and clearly understood.
NOTICE
NOTICE indicates instructions that must be followed to avoid
damage to the product or other equipment.
Notes and tips
A note is used to indicate information that is important for trouble-free and optimal
use of the product.
Note:
A tip contains useful information that can improve or optimize your procedures.
Tip:
Typographical conventions
Software items are identified in the text by bold italic text. A colon separates menu levels,
thus File:Open refers to the Open command in the File menu.
Hardware items are identified in the text by bold text (for example, Power).
6 Amersham Imager 600 Operating Instructions 29-0645-17 AD
1 Introduction
1.1 Important user information

1.2 Regulatory information
This section describes the directives and standards fulfilled by Amersham Imager 600.
Manufacturing information
The table below summarizes the required manufacturing information. For further infor-
mation, see the EU Declaration of Conformity (DoC) document.
ContentRequirement
GE Healthcare Bio-Sciences AB,Name and address of manufacturer
Björkgatan 30, SE 751 84 Uppsala, Sweden
Conformity with EU Directives
This product complies with the European directives listed in the table, by fulfilling the
corresponding harmonized standards.
A copy of the EU Declaration of Conformity is available on request.
TitleDirective
Machinery Directive (MD)2006/42/EC
Electromagnetic Compatibility (EMC) Directive2004/108/EC
International Standards
This product fulfills the requirements of the following standards:
NotesDescriptionStandard
EN 61010-1 lat-
est edition
aligned with EN
61010-2-081
Safety requirements for electrical
equipment for measurement, con-
trol, and laboratory use
IEC/EN 61010-1, IEC
61010-2-010, IEC 61010-
2-081, UL 61010-1,
CAN/CSA-C22.2 No.
61010-1
EN 61326-1, EN
301 489-1
aligned with
2004/108/EC
Electrical equipment for measure-
ment, control, and laboratory use -
EMC requirements
IEC/EN 61326-1, FCC Part
15 B Class A, ICES-003
Class A, EN 301 489-1, EN
301 489-17
Amersham Imager 600 Operating Instructions 29-0645-17 AD 7
1 Introduction
1.2 Regulatory information

NotesDescriptionStandard
Safety of machinery. General princi-
ples for design. Risk assessment and
risk reduction
EN ISO 12100
Degrees of protection provided by
enclosures
EN 60529
Standard for safety of flammability
of plastic materials for parts in de-
vices and appliances testing
UL94-V2
FCC compliance
This device complies with part 15 of the FCC Rules. Operation is subject to the following
two conditions: (1) This device may not cause harmful interference, and (2) this device
must accept any interference received, including interference that may cause undesired
operation.
The user is cautioned that any changes or modifications not expressly approved
by GE could void the user’s authority to operate the equipment.
Note:
This equipment has been tested and found to comply with the limits for a Class A digital
device, pursuant to part 15 of the FCC Rules. These limits are designed to provide rea-
sonable protection against harmful interference when the equipment is operated in a
commercial environment. This equipment generates, uses, and can radiate radio frequen-
cy energy and, if not installed and used in accordance with the instruction manual, may
cause harmful interference to radio communications. Operation of this equipment in a
residential area is likely to cause harmful interference in which case the user will be re-
quired to correct the interference at his own expense.
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1 Introduction
1.2 Regulatory information

CE marking
The CE marking and the corresponding EU Declaration of Conformity is valid for the in-
strument when it is:
•used as a stand-alone unit, or
•connected to other products recommended or described in the user documentation,
and
•used in the same state as it was delivered from GE, except for alterations described
in the user documentation.
Environmental Conformity
TitleDirective
Restriction of Hazardous Substances (RoHS) Directive2011/65/EU Annex II
Waste Electrical and Electronic Equipment (WEEE) Directive2012/19/EU
Administration on the Control of Pollution Caused by Elec-
tronic Information Products, China Restriction of Hazardous
Substances (RoHS)
ACPEIP
Registration, Evaluation, Authorization and restriction of
CHemicals (REACH)
Regulation (EC) No
1907/2006
Regulatory compliance of
connected equipment
Any electrical equipment connected to Amersham™ Imager 600 should meet the safety
requirements of EN/IEC 61010-1, or relevant harmonized standards. Within EU, connected
equipment must be CE marked.
Amersham Imager 600 Operating Instructions 29-0645-17 AD 9
1 Introduction
1.2 Regulatory information

Light sources
The safety of the LED light sources in this instrument has been assessed by risk analysis
in accordance with EN 61010-1.
Amersham Imager 600 , depending on configuration, is equipped with some or all light
sources in the table below:
WavelengthLight sources
460 nmBlue LED
520 nmGreen LED
630 nmRed LED
470 nm to 635 nmWhite LED
312 nmTrans-UV-illuminator
10 Amersham Imager 600 Operating Instructions 29-0645-17 AD
1 Introduction
1.2 Regulatory information

2 Safety instructions
About this chapter
This chapter describes safety precautions, safety labels, and emergency procedures for
the Amersham Imager 600 instrument, in addition to information on the safe disposal
of the instrument.
In this chapter
See pageSection
122.1 Safety precautions
222.2 Labels
252.3 Emergency procedure
262.4 Recycling information
272.5 Declaration of Hazardous Substances (DoHS)
Amersham Imager 600 Operating Instructions 29-0645-17 AD 11
2 Safety instructions

2.1 Safety precautions
Introduction
The Amersham Imager 600 instrument is powered by mains voltage, may be equipped
with a UV light source, and is used to capture images of samples that may be hazardous.
Before installing, operating or maintaining the instrument, you must be aware of the
hazards described in the user documentation. Follow the instructions provided to avoid
personal injury or damage to the instrument.
General precautions
WARNING
Do not operate Amersham Imager 600 in any other way than de-
scribed in the User Manual.
WARNING
Use of this equipment in other ways than those specified in the
user documentation may result in physical damage because of
exposure to irradiated light or electric shock or injury from touching
an operating part.
WARNING
Do not use the instrument if smoke, atypical noises or odors can
be perceived, or if the instrument becomes unusually hot as this
may result in fire or electric shock.
Stop using the instrument immediately, turn off the power switch,
and unplug the instrument from the power outlet. Contact your
local GE representative to request repair.
12 Amersham Imager 600 Operating Instructions 29-0645-17 AD
2 Safety instructions
2.1 Safety precautions

WARNING
Do not damage the power supply cord by bending, twisting, heating
or allowing them to become pinned under the equipment. Using
damaged power cords could result in fire or electric shock.
If the power supply cords are damaged, contact your local GE
representative for replacements.
WARNING
Access to power switch and power cord with plug. Do not block
access to the power switch and power cord. The power switch
must always be easy to access. The power cord with plug must
always be easy to disconnect.
WARNING
Do not block vents and ensure that they are kept free of dust and
dirt. Blockage can cause overheating and malfunctioning. To ensure
adequate cooling ensure there is at least 40 cm of free space in
front of the instrument and at least 10 cm on all other sides to walls
or other equipment.
Personal protection
WARNING
Hazardous substances. When using hazardous chemicals, take
all suitable protective measures, such as wearing protective
glasses and gloves resistant to the substances used. Follow local
and/or national regulations for safe operation and maintenance
of the system.
Amersham Imager 600 Operating Instructions 29-0645-17 AD 13
2 Safety instructions
2.1 Safety precautions

CAUTION
The instrument is equipped with a safety interlock. If the interlock
is out of order or has been tampered with, UV and visible light may
be emitted, which may cause skin burn and impair vision.
Do not open the instrument door during operation, when the RUN
indicator is lit. If the door is opened and the instrument keeps run-
ning, turn off the instrument immediately and contact GE service.
CAUTION
Do not touch the UV unit after UV exposure. There is a risk of skin
burn.
Installation
WARNING
As the equipment is heavy, contact a GE service person when
moving or relocating the main body of the equipment. If the
equipment drops, it may be broken, or you may get seriously injured
if it drops on your foot.
WARNING
Power cord. Only use power cords with approved plugs delivered
or approved by GE.
WARNING
Protective ground. Amersham Imager 600 must always be con-
nected to a grounded power outlet.
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2 Safety instructions
2.1 Safety precautions

WARNING
Use a proper power cord that complies with the local laws and
regulations, and is delivered by GE. Do not change power cord to
inappropriate one.
WARNING
In USA and Europe (Belgium, Netherlands, Luxemburg, Germany,
France, Italy, Denmark, Greece, Spain, Portugal, Austria, Finland,
Sweden, Poland, Hungary, Czech, Slovenia, Slovakia, Estonia, Latvia,
Lithuania, and Romania), use the proper power cord included in
the product package. In other countries, use the proper power cord
delivered separately from the product package by GE. Do not
change AC power cord to inappropriate one.
WARNING
Connect the power supply directly to a grounded wall power outlet.
The use of extension cords or multiple loads on one electrical outlet
could result in fire and electric shock.
CAUTION
Amersham Imager 600 must be secured before transport or relo-
cation. Contact GE for instructions before transporting the instru-
ment.
NOTICE
Do not connect telephone lines to the Ethernet port.
Only the IEC 60950-I/VL 60950-1 standard non-shielded cables are
appropriate for connection to this connector.
NOTICE
Only use IEC 60950-1-certified equipment and cables to connect
to the instrument.
Amersham Imager 600 Operating Instructions 29-0645-17 AD 15
2 Safety instructions
2.1 Safety precautions

Operation
WARNING
Do not use the instrument within or near a sink, or in humid (above
70% RH) or dusty environments. This can result in fire or electric
shock.
WARNING
In case of thunder, do not touch the power supply plug, as this can
result in electric shock.
CAUTION
Always use appropriate Personal Protective Equipment (PPE) during
operation and maintenance of Amersham Imager 600.
•Protective eyewear
•Protective gloves
•Protective footware, preferably with steel lining
CAUTION
Reagents used to prepare the sample should be used in accordance
with the manufacturer instructions.
CAUTION
Do not lean on the sample door.
CAUTION
Use the handle to open or close the instrument door.
16 Amersham Imager 600 Operating Instructions 29-0645-17 AD
2 Safety instructions
2.1 Safety precautions

CAUTION
Do not place heavy objects on top of the instrument.
CAUTION
Handle sample trays and tablet computers with care to avoid
dropping them.
CAUTION
Do not touch the light source in the instrument. The light source
may be hot.
CAUTION
Do not leave samples in the instrument after exposure. If left, these
may degrade and cause damage to the instrument.
CAUTION
Do not disable the interlock of the equipment as UV light can be
emitted if the door is opened.
NOTICE
Both the Amersham Imager 600 Control Software and Amersham
Imager 600 Analysis Software do not analyze the image files other
than those captured and generated by Amersham Imager 600.
Analysis of files other than those captured on the Amersham Imager
600 may introduce errors.
Amersham Imager 600 Operating Instructions 29-0645-17 AD 17
2 Safety instructions
2.1 Safety precautions

NOTICE
Do not add and/or remove any files in the folder generated by
Amersham Imager 600 with Amersham Imager 600 Control
Software and Amersham Imager 600 Analysis Software. Do not
modify any folders in which files have been added or removed. This
may prevent a correct analysis afterwards.
NOTICE
Do not use USB ports for recharging external devices. This can
cause instrument malfunction.
NOTICE
Connection of multiple computers to one instrument may impair
performance. Simultaneous use can cause loss of data.
NOTICE
Avoid connecting additional accessories such as computers,
monitors or keyboards to the instrument during operation as this
can stop the exposure or cause loss of data.
NOTICE
Do not turn off power during operation as this can cause loss of
data and damage the Compact flash drive. Only turn off power in
an emergency situation.
NOTICE
Do not open the instrument door during exposure, when the RUN
light indicates operation. This will stop the exposure and data will
not be saved.
18 Amersham Imager 600 Operating Instructions 29-0645-17 AD
2 Safety instructions
2.1 Safety precautions

NOTICE
Transferring large amounts of files to a connected computer during
operation can impair the system’s performance.
NOTICE
Do not touch the light source windows as scratches or dirt may
impair performance.
NOTICE
The tablet computer cannot be recharged with this instrument.
NOTICE
When connecting an external monitor to the system, use a monitor
with SVGA resolution. With a low-resolution monitor, operation
buttons cannot be seen.
NOTICE
Remove all traces of acidic solutions to prevent corrosion in the
equipment.
Maintenance
WARNING
Electrical shock hazard. All instrument repairs or modifications
should be performed by service personnel authorized by GE. Do
not open any covers or replace parts unless specifically stated in
the user documentation.
Amersham Imager 600 Operating Instructions 29-0645-17 AD 19
2 Safety instructions
2.1 Safety precautions

WARNING
Do not use excessive amounts of liquids for cleaning the Amersham
Imager 600 instrument. This may result in instrument malfunction
or electric shock.
CAUTION
Always use appropriate Personal Protective Equipment (PPE) during
operation and maintenance of Amersham Imager 600.
•Protective eyewear
•Protective gloves
•Protective footware, preferably with steel lining
CAUTION
Turn off the power switch before cleaning the inside of the instru-
ment.
CAUTION
UV lamps in Amersham Imager configurations 600 UV, 600 QC,
and 600 RGB contain mercury, which must be recycled or disposed
of in accordance with local regulations.
NOTICE
Do not use abrasive cleaning materials, such as a scouring pad,
for cleaning. This may cause damage to the instrument.
NOTICE
Dispose of the tablet computer as instructed in its user manual.
20 Amersham Imager 600 Operating Instructions 29-0645-17 AD
2 Safety instructions
2.1 Safety precautions