Gilson pipetman P10 Series User manual

User’s Guide
EN

2| PIPETMAN®
TABLE OF CONTENTS
INTRODUCTION | 3
Parts Checklist | 3
Good Laboratory Practice (GLP) Compliance | 3
Description | 4
Specifications | 4
SETTING THE VOLUME | 6
PIPETTING | 6
Fitting the Tips | 6
Pre-Wetting the Tips | 8
Aspirating | 8
Dispensing | 8
Guidelines for Good Pipetting | 9
ACCESSORIES | 10
TROUBLESHOOTING | 12
Troubleshooting Table l 12
Leak Test | 13
MAINTENANCE | 13
Maintenance for Single Channel Models | 13
Maintenance for Multichannel Models | 15
CLEANING AND DECONTAMINATION | 16
Cleaning for Single Channel Models | 16
Cleaning for Multichannel Models l 16
Autoclaving l 16
Chemical Decontamination l 17
SPARE PARTS | 18
WARRANTY | 20
TABLE OF CONTENTS
Decreased pipetting forces are due to the design of the piston assembly, which includes a very high-quality
lubricant (part number 5440011070). The use of any other lubricant voids the warranty of this pipette.
NOTICE

INTRODUCTION
3
PIPETMAN® |USER’S GUIDE
Chapter 1
INTRODUCTION
PIPETMAN®, recognized as the pipetting standard, is a fully adjustable, air-displacement pipette that uses disposable tips.
PIPETMAN combines legendary accuracy, precision, and durability with easy pipetting and handling. PIPETMAN pipettes
come in a large range of volumes with reduced pipetting forces for hours of comfortable pipetting.
Eight single channel models cover a volume range from 0.2 µL to 10 mL.
Eight multichannel models cover a volume range from 1 µL to 300 µL.
Parts Checklist
After unpacking the pipette, verify that the following items were included and are undamaged:
Single Channel Models
●PIPETMAN®
●Quick Guide
●Safety bag
●Certificate of Conformity (including barcode sticker)
●Dual-position adapter (for P2 and P10 models only)
Multichannel Models
●PIPETMAN®
●Quick Guide
●Safety bag
●Certificate of Conformity (including barcode sticker)
●Ejector spacers for D10 tips (for Px10 models only)
Good Laboratory Practice (GLP) Compliance
The serial number is engraved on the body of the pipette. It provides unique identification of your pipette and the
manufacturing date.
Serial number
Figure 1
Serial number and identification
Year Month Production
number
Ex: A A 50001 YEAR CODE
2022 T
2023 U
2024 W
2025 X
2026 Y
2027 Z
MONTH CODE
January A
February B
March C
April D
May E
June G
July H
August J
September K
October L
November M
December N
The barcode on the box and the Certificate of Conformity provides traceability of your pipette.

INTRODUCTION
4INTRODUCTION | PIPETMAN®
Description
Figure 2
PIPETMAN® multichannel and single channel model components
Color-coded
push button
Tip ejector button
Thumbwheel
Blue ring
Body or handle
Clip tip ejector
Connecting nut
Ejector lock
Cover
Tip holder
Ejector spacer
PIPETMAN®
DIAMOND Tips
Please refer to the following chapters for a full description of the different parts and functions of the pipette.
The data given in the tables conform to the ISO 8655-2 standard.
With a precise pipetting technique, the P2 model may be used to aspirate volumes as low as 0.1 µL and the
P10 model as low as 0.5 µL.
NOTICE
Specifications
PIPETMAN® is a high quality pipette that offers excellent accuracy and precision. The data provided in the Gilson Maximum
Permissible Errors table were obtained using PIPETMAN® DIAMOND Tips. These values are guaranteed only when genuine
PIPETMAN DIAMOND Tips are used.
Each pipette is inspected and validated by qualified technicians in accordance with the Gilson Quality System. Gilson declares
that its manufactured pipettes comply with the requirements of
the ISO 8655 standard by type testing.
The adjustment is carried out under strictly defined and monitored conditions (ISO 8655-6).
PIPETMAN® is equipped with a stainless steel tip ejector and can also be used with a plastic tip ejector, which is
available as an accessory (refer to ACCESSORIES on page 10).

INTRODUCTION
5
PIPETMAN® |USER’S GUIDE
Maximum Permissible Errors
*CV means Coecient of Variation
**P10 model can be used up to 0.5 μL with a precise pipetting technique.
***P5000 and P10mL do not have tip ejectors.
Gilson maximum permissible errors are guaranteed only when PIPETMAN® pipettes are used with the recommended PIPETMAN® DIAMOND Tips.
PIPETMAN® SINGLE CHANNEL—VARIABLE VOLUME MODELS
Model PIPETMAN®
DIAMOND Tips
Part Number Vol.
(µL)
Maximum Permissible Errors
Gilson ISO 8655
Systematic
Error
(µL)
Random
Error
(µL)
Systematic
Error
(%)
Random
Error
(%CV*)
Systematic
Error
(µL)
Random
Error
(µL)
Systematic
Error
(%)
Random
Error
(%CV*)
P2 D10
DL10 F144054M
0.2
0.5
1
2
± 0.024
± 0.025
± 0.025
± 0.030
≤0.012
≤0.012
≤0.012
≤0.014
± 12.0
± 5.0
± 2.5
± 1.5
≤6.0
≤2.4
≤1.3
≤0.7
± 0.08
± 0.08
± 0.08
± 0.08
≤0.04
≤0.04
≤0.04
≤0.04
± 40.0
± 16.0
± 8.0
± 4.0
≤20.0
≤8.0
≤4.0
≤2.0
P10** D10
DL10 F144055M
1
5
10
± 0.025
± 0.075
± 0.100
≤0.012
≤0.030
≤0.040
± 2.5
± 1.5
± 1.0
≤1.2
≤0.6
≤0.4
± 0.12
± 0.12
± 0.12
≤0.08
≤0.08
≤0.08
± 12.0
± 2.4
± 1.2
≤8.0
≤1.6
≤0.8
P20 D200 F144056M
2
10
20
± 0.10
± 0.10
± 0.20
≤0.030
≤0.050
≤0.060
± 5.0
± 1.0
± 1.0
≤1.5
≤0.5
≤0.3
± 0.2
± 0.2
± 0.2
≤0.1
≤0.1
≤0.1
± 10.0
± 2.0
± 1.0
≤5.0
≤1.0
≤0.5
P100 D200 F144057M
10
50
100
± 0.35
± 0.40
± 0.80
≤0.10
≤0.12
≤0.15
± 3.5
± 0.8
± 0.8
≤1.0
≤0.24
≤0.15
± 0.8
± 0.8
± 0.8
≤0.3
≤0.3
≤0.3
± 8.0
± 1.6
± 0.8
≤3.0
≤0.6
≤0.3
P200 D200 F144058M
20
100
200
± 0.50
± 0.80
± 1.60
≤0.20
≤0.25
≤0.30
± 2.5
± 0.8
± 0.8
≤1.0
≤0.25
≤0.15
± 1.6
± 1.6
± 1.6
≤0.6
≤0.6
≤0.6
± 8.0
± 1.6
± 0.8
≤3.0
≤0.6
≤0.3
P1000 D1000 F144059M
100
500
1000
± 3.0
± 4.0
± 8.0
≤0.6
≤1.0
≤1.5
± 3.0
± 0.8
± 0.8
≤0.6
≤0.2
≤0.15
± 8.0
± 8.0
± 8.0
≤3.0
≤3.0
≤3.0
± 8.0
± 1.6
± 0.8
≤3.0
≤0.6
≤0.3
P5000*** D5000 F144066
500
2500
5000
± 12
± 15
± 30
≤3
≤5
≤8
± 2.4
± 0.6
± 0.6
≤0.6
≤0.2
≤0.16
± 40
± 40
± 40
≤15.0
≤15.0
≤15.0
± 8.0
± 1.6
± 0.8
≤3.0
≤0.6
≤0.3
P10mL*** D10mL F144067
1000
5000
10000
± 30
± 40
± 60
≤6
≤10
≤16
± 3.0
± 0.8
± 0.6
≤0.6
≤0.2
≤0.16
± 60
± 60
± 60
≤30.0
≤30.0
≤30.0
± 6.0
± 1.2
± 0.6
≤3.0
≤0.6
≤0.3
PIPETMAN® MULTICHANNEL
P8x10
P12x10
D10
DL10
F144068
F144069
1
5
10
± 0.08
± 0.20
± 0.20
≤0.05
≤0.10
≤0.10
± 8.0
± 4.0
± 2.0
≤5.0
≤2.0
≤1.0
± 0.24
± 0.24
± 0.24
≤0.16
≤0.16
≤0.16
± 24.0
± 4.8
± 2.4
≤16.0
≤3.2
≤1.6
P8x20
P12x20
DL10
D200
F144070
F144071
2
10
20
± 0.10
± 0.20
± 0.40
≤0.08
≤0.10
≤0.20
± 5.0
± 2.0
± 2.0
≤4.0
≤1.0
≤1.0
± 0.4
± 0.4
± 0.4
≤0.20
≤0.20
≤0.20
± 20.0
± 4.0
± 2.0
≤10.0
≤2.0
≤1.0
P8x200
P12x200
D200 F144072
F144073
20
100
200
± 0.50
± 1.00
± 2.00
≤0.25
≤0.40
≤0.50
± 2.5
± 1.0
± 1.0
≤1.25
≤0.4
≤0.25
± 3.2
± 3.2
± 3.2
≤1.2
≤1.2
≤1.2
± 16.0
± 3.2
± 1.6
≤6.0
≤1.2
≤0.6
P8x300
P12x300 D300
F144074
F144075
30
150
300
± 1.00
± 1.50
± 3.00
≤0.35
≤0.60
≤1.00
± 3.33
± 1.0
± 1.0
≤1.17
≤0.4
≤0.33
± 8.0
± 8.0
± 8.0
≤3.0
≤3.0
≤3.0
± 26.67
± 5.33
± 2.67
≤10.0
≤2.0
≤1.0

SETTING THE VOLUME
6SETTING THE VOLUME | PIPETMAN®
Figure 3
Dial colors by model
1
5
P2
1.25 µL
2
0
7
5
P10
7.5 µL
1
2
5
P20
12.5 µL
P100
0
7
5
75 µL
P200
1
2
5
125 µL
P1000
0
7
5
0.75 mL
P5000
1
2
5
1.25 mL
P10mL
0
7
5
7.5 mL
0
7
5
Px10
7.5 µL
1
2
5
Px20
12.5 µL
Px200
1
2
5
125 µL
Px300
1
2
5
125 µL
Push button
Thumbwheel
Figure 4
Location of push button
and thumbwheel
MODEL
COLOR OF VOLUMETER NUMBERS
BLACK RED INCREMENT
P2 µL 0.01 µL 0.002 µL
P10 - P20 - PX10 - PX20 µL 0.1 µL 0.02 µL
P100 - P200 - PX200 - PX300 µL - 0.2 µL
P1000 0.01 mL mL 0.002 mL
P5000 0.01 mL mL 0.002 mL
P10mL mL 0.1 mL 0.02 mL
To achieve maximum accuracy when setting the volume, proceed as follows:
●When decreasing the volume setting, slowly reach
the required setting, making sure to not overshoot
the mark.
●When increasing the volume setting, pass the
required value by 1/3 of a turn and then slowly
decrease to reach the volume, making sure to not
overshoot the mark.
Chapter 2
SETTING THE VOLUME
The volume of liquid to be aspirated is set using the volume display. The dial colors are either black or red to
indicate the position of the decimal point, depending on the model (refer to Figure 3).
The volume is set by turning the thumbwheel or the push button (refer to Figure 4). The push button makes it
easier and quicker to set volumes, especially when wearing gloves. The thumbwheel may be turned using only one
hand to slowly reach the required setting.
Chapter 3
PIPETTING
While many brands of tips can be used with PIPETMAN®, it is recommended to use PIPETMAN® DIAMOND Tips with
PIPETMAN® for optimum performance. These tips are made from pure polypropylene. Plastic tips are for a single
application—they should not be cleaned for reuse.
Fitting the Tips
Single Channel Models
To fit a new PIPETMAN DIAMOND Tip, push the tip holder into the tip using a slight twisting motion to ensure a firm,
airtight seal.
Figure 5
PIPETMAN® DIAMOND Tip compatibility chart for single
channel models
PIPETMAN DIAMOND Tip
Compatibility for single channel
variable volume models
P2, P10 D10, DL10, DF10ST, DFL10ST
P20 D200, DF30ST
P100 D200, DF100ST
P200 D200, D300, DF200ST, DF300ST
P1000 D1000, D1200, DF1000ST, DF1200ST
P5000 D5000
P10mL D10mL

PIPETTING
7
PIPETMAN® |USER’S GUIDE
NOTE
For the P2 and P10 models, a dual-position adapter (plastic) is required to fit DL10 tips (longtips) or D10 tips
(short tips). The metallic rod of the tip ejector is shaped so the adapter may be clipped to it in either position.
P2 and P10 models are delivered with the adapter in place, ready to use DL10 tips. If D10 tips are
used, the adapter must be repositioned in the shorter slot as follows:
1. Pull the adapter down from the metallic rod.
2. Turn the adapter 180°.
3. Refit the adapter so the end of the metallic rod engages the shorter slot of the adapter
Short tips Long tips
Figure 6
Dual-position
adapter for P2
and P10 with
stainless steel tip
ejector
Fitting the Tips on P2 and P10 models
D5000 and D10mL PIPETMAN DIAMOND tips do not have any filter. P5000 and P10mL models are sold with a
bag of 10 filters. Bags of filters are also available as accessories. (refer to Accessories on page 10).
The filters, made of polyethylene, oer the same properties as the filters which are inside the PIPETMAN
DIAMOND filter tips. They maintain the pipette’s original accuracy and precision by creating an ecient barrier
to aerosols or corrosive vapors such as acids which could damage mechanical parts.
Insert the filter directly in the pipette tip holder.
Change the filter every day or more frequently depending on the intensity of use and/or concentration of the
solution.
Filters are not autoclavable and must be disposed of after use.
NOTICE
The dual-position adapter is autoclavable.
An autoclavable tip ejector extension can be ordered as an accessory for P2 and P10 equipped with a
plastic tip ejector (part number F107027; refer to ACCESSORIES on page 10).
To fit an extension to plastic ejector:
1. Slide the extension over the tip holder.
2. Push the extension firmly onto the end of the tip ejector until it clicks into place.
To remove an extension from plastic ejector:
1. Gently twist the extension.
2. Pull it away from the pipette.
PIPETMAN® DIAMOND Tips are best fitted with the ROCKY RACK technique, invented by Gilson, available only in our
TIPACK and TOWERPACK.
Figure 7
ROCKY RACK
technique
PIPETMAN DIAMOND Tip Compatibility
for multichannel models
P8x10, P12x10 D10*, DL10, DF10, DFL10
P8x20, P12x20 DL10, DFL10, DF30, D200
P8x200, P12x200 D200, D300, DF200, DF300
P8x300, P12x300 D200, D300, DF200, DF300
Figure 8
PIPETMAN DIAMOND Tips compatibility chart for
multichannel models
Multichannel models
NOTE
* Using the broad ejector spacer D10, you can adapt a D10 tip (see below)

PIPETTING
8PIPETTING | PIPETMAN®
Pre-Wetting the Tips
Pre-wetting the tips before pipetting helps prepare the tips for the best pipetting performance. Ideally, the pre-wet
includes both immersing the tip in the liquid and performing one pipetting step.
Pre-wetting the tips helps ensure that volumes that your pipette will achieve accuracy and precision within
specifications.
First stop
Second stop
Rest position
Figure 10
Pipetting motion - aspirate and dispense
Dispensing
1. Place the end of the tip against the inside wall of the
recipient vessel (at an angle of 10° to 40°).
2. Press the push button slowly and smoothly to the
first stop.
3. Wait for at least one second, then press the push
button to the second stop to expel any residual
liquid from the tip. Keep the push button pressed
fully down and (while removing the pipette) draw
the tip along the inside surface of the vessel.
4. Release the push button smoothly. Eject the tip by
pressing firmly on the tip ejector button.
Ejector Spacer for PIPETMAN® 10 µL Multichannel Models
According to the tip used, D10 or DL10, you may have to exchange the ejector spacer.
The broad one is dedicated to D10, and the small one is dedicated to DL10.
●Remove the tip ejector, keeping both ejector locks depressed. Pull the tip ejector down.
●Gently press the tabs from the ejector spacer and remove it from the tip ejector.
●Insert the alternative ejector spacer and click it to the tip ejector.
●To refit the tip ejector, gently re-insert the tip ejector vertically into the rails of the ejector
support.
Ejector
spacer D10
Short tips
Long tips
Ejector
spacer
Figure 9
Ejector spacer location
Aspirating
1. Press the push button to the first stop (this
corresponds to the set volume of liquid).
2. Hold the pipette vertically and immerse the tip in
the liquid (refer to Guidelines for Good Pipetting
on page 9).
3. Release the push button slowly and smoothly (to
the top position) to aspirate the set volume of
liquid. Wait one second (refer to Guidelines for
Good Pipetting on page 9) and then withdraw the
pipette-tip from the liquid.
4. You may wipe any droplets away from the outside
of the tip using a medical wipe, however, if you do
so, take care to avoid touching the tip’s orifice.

PIPETTING
9
PIPETMAN® |USER’S GUIDE
Guidelines for Good Pipetting
Make sure that you operate the push button slowly and smoothly.
When aspirating, keep the tip at a constant depth below the surface of the liquid (refer to the table below).
IMMERSION DEPTH AND WAIT TIME
MODEL IMMERSION
DEPTH (MM)
WAIT TIME
(SECONDS)
P2 1 1
P10 - Px10 1 1
P20 - Px20 2-3 1
P100 - Px200 - Px300 2-4 1
P200 2-4 1
P1000 2-4 2-3
P5000 3-6 4-5
P10mL 5-7 4-5
●Change the tip before aspirating a different liquid, sample, or reagent.
●Change the tip if a droplet remains at the end of the tip from the previous pipetting operation.
●Pre-wet each new tip with the liquid to be pipetted.
●Liquid should never enter the tip holder.
To prevent this:
○Press and release the push button slowly and smoothly.
○Never turn the pipette upside down.
○Never lay the pipette on its side when there is liquid in the tip.
●If you use the same tip with a larger volume, pre-wet the tip.
●For volatile solvents, you should saturate the air cushion of your pipette by aspirating and dispensing the solvent
repeatedly before aspirating the sample.
●When the temperature of the liquid is different from the ambient temperature, pre-wet the tip several times before
use.
●You may remove the tip ejector (refer to MAINTENANCE on page 13) to aspirate from very narrow tubes.
●After pipetting acids or other corrosive liquids that emit vapors, remove the tip ejector and the tip holder; and then
rinse, dry, and lubricate the piston (refer to MAINTENANCE on page 13). For the model P1000, you can increase the
lifetime of the piston by using a specific tip holder equipped with a filter (refer to ACCESSORIES on page 10).
●Do not pipette liquids with temperatures above 70°C or below 4°C. The pipette can be used between 4°and 40°C,
but the specifications may vary according to the temperature (refer to Maximum Permissible Errors on page 5).
It is recommended to regularly verify the volume displayed on the dial while pipetting.
PIPETMAN should be held in the vertical position.
NOTICE

ACCESSORIES
10 ACCESSORIES | PIPETMAN®
Chapter 4
ACCESSORIES
DESCRIPTION PART NUMBER
Plastic ejector P2/P10 with adapter F107027
Plastic ejector P20 F107028
Plastic ejector P100 F107029
Plastic ejector P200 F107030
Plastic ejector P1000 F107031
To make pipetting more comfortable and more convenient, Gilson has developed several accessories:
Pipette stands allow users to store pipettes vertically to avoid the possibility of liquid running back into the pipette.
CARROUSEL™ Pipette Stand
(7 pipettes) F161401
TRIO™ Stand (3 pipettes) F161405
Universal Multichannel Stand* F161417
SINGLE™ Pipette Holder F161406
DESCRIPTION PART NUMBER
*Universal multichannel stand is for multichannel models only.
To identify or personalize your pipette, COLORISTM Identification Clips are available.
COLORIS clips
(mixed colors set of 10) F161301
COLORIS clips (red, set of 10) F161302
COLORIS clips (yellow, set of 10) F161303
COLORIS clips (green, set of 10) F161304
COLORIS clips (blue, set of 10) F161305
COLORIS clips (white, set of 10) F161306
DESCRIPTION PART NUMBER
With The JIMMY™ Microtube Opener, a hands-free microtube opener, you can open both snap-cap and screw-cap
microtubes.
DESCRIPTION PART NUMBER
JIMMY Microtube Opener
(set of 3) F144983
SINGLE™
CARROUSEL™ TRIO™
Universal
Multichannel
Stand
Plastic tip ejector

ACCESSORIES
11
PIPETMAN® |USER’S GUIDE
For P5000 and P10mL models, disposable filters maintain the pipette’s original accuracy and precision by creating an
efficient barrier to aerosols or corrosive vapours such as acids which could damage mechanical parts.
DESCRIPTION PART NUMBER
Bag of 10 Filters for
P5000/P10mL F161280
Bag of 100 Filters for
P5000/P10mL F161241
DESCRIPTION PART NUMBER
PIPETMAN® COMFORT HANDLE
(pack of 3, blue) F161486
PIPETMAN® COMFORT HANDLE
(pack of 3, white) F161488
PIPETMAN® COMFORT HANDLE
(pack of 50, blue) F161485
PIPETMAN® COMFORT HANDLE
(pack of 50, white) F161487
PIPETMAN® COMFORT HANDLE
(stickers, 120) F161499
PIPETMAN® COMFORT HANDLE models reduce the force needed to grip the pipette for maximum comfort.

TROUBLESHOOTING
12 TROUBLESHOOTING | PIPETMAN®
Symptom Possible Cause Action Model*
Pipette is leaking
sample
Damaged tip holder Replace the tip holder S
Worn O-ring or seal Replace both parts and lubricate S
Pipette will not
aspirate
Worn O-ring Replace both parts and lubricate S
Damaged tip holder Replace the tip holder S
Connecting nut is loose Tighten connecting nut S+M
Damaged or corroded piston Return pipette to supplier S
Improper repair or assembly Refer to page 13 - MAINTENANCE S
Pipette volume is
inaccurate
Improper repair or assembly Refer to page 13 - MAINTENANCE S
Connecting nut is loose Tighten connecting nut S+M
Tip holder is loose Tighten connecting nut S
Incorrect operator technique Operator training S+M
Damaged or corroded piston(s) Return pipette to supplier S
Damaged tip holder(s) Replace the tip holder(s) S
Worn O-ring or seal Replace both parts and lubricate S
Tips fall o or do not
fit correctly
Low quality tips Use PIPETMAN DIAMOND Tips S+M
Damaged tip holder(s) Replace the tip holder(s) S+M
Damaged tip ejector Replace the tip ejector S+M
Dirty tip holder Clean the tip holder with isopropanol or
ethanol S+M
The tip ejector is loose Assemble the tip ejector properly S
The ejector lock is misaligned Align the ejector lock S
Pipetting seize up Piston needs lubricant Lubricate piston assembly S
*S: Single channel pipettes / M: Multichannel pipettes
NOTE
If you cannot solve the problem, contact your local Gilson-authorized Service Center.
For Single Channel Variable, Fixed Volume, and Multichannel Models
Chapter 5
TROUBLESHOOTING
A quick inspection of the pipette may help you detect a potential problem.
NOTE
You may download the Two Minute Inspection Poster from the Gilson website (www.gilson.com). The poster
describes how to perform a quick diagnosis of your pipette.
WARNING
Before returning any pipette to your local Gilson Service Center, ensure that it is completely free of
chemical, biological, or radioactive contamination (refer to CLEANING AND DECONTAMINATION on page
16). Please use the included safety bag to return the pipette to your local Gilson Service Center.
Troubleshooting Table
The following table may help you identify and correct the potential problem you might encounter.
NOTE
Please make sure the tips are correctly fitted. Refer to Fitting the Tips on page 6.

MAINTENANCE
13
PIPETMAN® |USER’S GUIDE
Leak Test
This test may be performed at any time to check that the pipette does not leak, especially after performing a
maintenance or decontamination procedure. If a pipette fails this test, replace the O-ring and seal.
After making sure that the pipette is correctly reassembled, repeat this test.
For the P2 to P200 Models
1. Fit a PIPETMAN® DIAMOND Tip.
2. Set the pipette to the maximum volume given in the specifications and pre-wet.
3. Aspirate the set volume from a beaker of distilled water.
4. Maintain the pipette in the vertical position and wait for 20 seconds.
●If a water droplet appears at the end of the tip, there is a leak.
●If you see no droplet, re-immerse the tip below the surface of water.
The water level inside the tip should remain constant. If the level goes down, there is a leak.
For the P1000, P5000, and P10mL Models
1. Fit a PIPETMAN DIAMOND Tip.
2. Set the pipette to the maximum volume given in the specifications.
3. Aspirate the set volume from a beaker of distilled water.
4. Maintain the pipette in the vertical position and wait for 20 seconds.
●If a water droplet appears at the end of the tip, there is a leak.
For the Multichannel Models (P8x, P12x)
1. Fit the PIPETMAN® DIAMOND Tip.
2. Set the pipette to the maximum volume given in the specifications and pre-wet.
3. Aspirate the set volume from a reagent reservoir of distilled water.
4. Maintain the pipette in the vertical position and wait for 20 seconds.
●If a water droplet appears at the end of the tip, there is a leak.
●For volumes below 200 μL, if you see no droplets, re-immerse the tip below the surface of water.
●The water level inside the tip should remain constant. If the level goes down, there is a leak.
Chapter 6
MAINTENANCE
Routine maintenance will help keep your pipette in good condition, ensuring a continued high level of performance.
NOTICE
Gilson recommends maintenance and calibration at least annually, more frequently as needed, depending on use.
The instructions provided below detail how to perform some basic maintenance yourself. Please contact your local
Gilson service provider (Click here to find your local Gilson representative) to have Gilson provide maintenance
and calibration for you or to ask any service related questions.
Maintenance is limited to:
●Cleaning or decontamination (refer to CLEANING AND DECONTAMINATION on page 16).
●Replacing spare parts
●Greasing the piston assembly
Maintenance for Single Channel Models
PIPETMAN P2 and P10 should not be disassembled, so you can only replace the push button, tip ejector, and its dual
position adapter. With these pipettes if the tip holder is damaged, the piston may also be damaged.

MAINTENANCE
14 MAINTENANCE | PIPETMAN®
After replacing any parts, you should verify the performance of your pipette following the verification procedure
available on the Gilson website (www.gilson.com). If the pipette needs to be readjusted, please contact your local
Gilson Service Center.
Changing the Tip Ejector for Single Channel Models
To remove:
Push the ejection button.
Laterally push the tip ejector.
Slide and remove the tip ejector.
To refit:
Push the ejection button.
Slide the tip ejector along the tip holder.
Push laterally to clip the tip ejector on the body of the pipette.
1
2
3
1
2
3
1
2
3
Changing the Tip Holder – No Tools Required
1. Remove the tip ejector (refer to Changing the Tip Ejector for
Single Channel Models on page 14).
2. Unscrew the connecting nut by turning it counterclockwise.
3. Carefully separate the lower and upper parts.
4. Remove the piston assembly and the seals.
5. Clean, autoclave, or replace the tip holder.
6. If necessary, lightly lubricate the piston and its seals (refer to
Servicing the Piston Assembly on page 14).
7. Reassemble the pipette (refer to the Figure 11).
8. Tighten the connecting nut (turn clockwise)
9. Refit the tip ejector (refer to Changing the Tip Ejector for Single
Channel Models on page 14).
P10, P20
P100, P200
P5000
P10mL
P1000
Tip ejector
Stainless
steel
Plastic tip ejector
available as an
acessory
Connecting nut
Color-coded
push button
Piston
assembly
Blue ring
Figure 11
Disassembled PIPETMAN®, with different piston
assemblies
Servicing the Piston Assembly
You may remove the piston assembly for cleaning purposes only. If
the piston assembly is changed, the pipette must be adjusted and
calibrated by a trained technician in a Gilson-authorized Service
Center. Because P2 and P10 models contain miniaturized parts, it
is best not to disassemble these pipettes yourself.
The piston assembly must not be autoclaved.
NOTICE
1. Remove the tip ejector (refer to Changing the Tip Ejector for Single Channel
Models on page 14).
2. Unscrew the connecting nut by turning it clockwise.
3. Carefully separate the lower and upper parts.
4. Remove the piston assembly and the seals.
5. Leave the piston exposed, clean it with isopropanol or ethanol, and lubricate
lightly. For P20 to P10mL models, lubricate only the internal part of the piston
and the seals.
NOTICE

MAINTENANCE
15
PIPETMAN® |USER’S GUIDE
Optimized pipetting forces are due to the design of the piston assembly, which includes a very high-quality lubricant
(part number 5440011070). The use of any other lubricant voids the warranty of this pipette.
NOTICE
6. Reassemble the pipette (refer to Figure 11 on page 14).
7. Tighten the connecting nut (turn counterclockwise).
8. Refit the tip ejector (refer to Changing the Tip Ejector for Single Channel Models on page 14).
Changing the Seal Guide (or Seal)
The O-ring and seal on the piston must not be autoclaved. If worn or damaged in any way (chemical or mechanical), they
must be replaced. Because the models P2 and P10 contain miniaturized parts, it is best not to disassemble these pipettes
yourself. Please contact your local Gilson Service Center.
The dimensions of the O-ring vary depending on the model of pipette.
1. Remove the tip ejector (refer to Changing the Tip Ejector for Single Channel Models on page 14).
2. Unscrew the connecting nut by turning it clockwise.
3. Carefully separate the lower and upper parts.
4. Remove the piston assembly, O-ring, and seal.
5. If necessary, clean the piston and replace the seal. Lightly lubricate both seal and piston. Remember to replace the seal and
O-ring in the correct order.
6. Reassemble the pipette (refer to the Figure 11 on page 14).
7. Tighten the connecting nut (turn counterclockwise).
8. Refit the tip ejector (refer to Changing the Tip Ejector for Single Channel Models on page 14).
Maintenance for Multichannel Models
Routine maintenance will help keep your pipette in good condition, ensuring a continued high level of performance.
Maintenance is limited to:
●Cleaning or decontamination (refer to CLEANING AND DECONTAMINATION on page 16).
●Replacing spare parts
●Greasing the piston assembly
Changing the Tip Ejector for Mutichannel Model
To remove the tip ejector, keep both ejector locks depressed. Pull the tip ejector down.
To refit the tip ejector, gently re-insert the tip ejector vertically into the rails of the ejector support.
Pull lightly on the tip ejector to check the position.
Tip ejector Ejector lock
Ejector spacer
Figure 12
PIPETMAN® multichannel tip ejector and ejector spacers

CLEANING AND DECONTAMINATION
16 CLEANING AND DECONTAMINATION | PIPETMAN®
Chapter 7
CLEANING AND DECONTAMINATION
PIPETMAN®is designed so that the parts normally in contact with liquid contaminants can easily be cleaned and
decontaminated. However, because the models P2 and P10 contain miniaturized parts, it is best not to disassemble
these pipettes yourself. Please contact your local Gilson Service Center.
NOTICE
You can refer to the decontamination procedure available on the Gilson website (www.gilson.com).
Liquid must never enter the upper part (handle) of any pipette.
Cleaning for Single Channel Models
The pipette must be cleaned, as described below, before it is decontaminated. Soap solution is recommended for
cleaning PIPETMAN®.
External
1. Remove the tip ejector (refer to Changing the Tip Ejector for Single Channel Models on page 14).
2. Wipe the entire pipette and the tip ejector with a soft cloth or lint-free tissue soaked with soap solution to remove all
dirty marks. If the pipette is very dirty, a brush with soft plastic bristles may be used.
3. Wipe the entire pipette and the tip ejector with a soft cloth or lint-free tissue soaked with distilled water.
4. Refit the tip ejector and allow the pipette to dry.
Internal
Only the following components can be immersed in a cleaning solution: connecting nut, tip ejector, tip holder, piston
assembly, seal, and O-ring.
1. Disassemble the pipette (refer to Figure 11 on page 14).
2. Set aside the upper part in a clean, dry place.
3. Clean the individual components of the lower part of the pipette using an ultrasonic bath (20 minutes at 50°C) or with a
soft cloth and brushes.
NOTE
The piston assembly and seals must be degreased with isopropanol or ethanol before being immersed in the ultrasonic
bath. Small round brushes with soft plastic bristles may be used to clean the interior of the tip holder.
4. Rinse the individual components with distilled water.
5. Let the parts dry by evaporation or wipe them with a clean soft cloth or lint-free tissue.
6. Reassemble the pipette as described (refer to Figure 11 on page 14).
Cleaning for Multichannel Models
Only the following components can be immersed in a cleaning solution: tip ejector, ejector locks, and ejector spacer.
1. Remove the tip ejector and the ejector spacer.
2. Immerse the tip ejector, ejector locks, and ejector spacer in the cleaning solution or wipe them with a soft cloth or
lint-free tissue soaked with the cleaning solution.
3. Rinse the components with distilled water.
4. Wipe the entire pipette with a soft cloth or lint-free tissue soaked with the cleaning solution.
5. Wipe it with distilled water.
6. Let the parts dry by evaporation or wipe them with a clean soft cloth or lint-free tissue.
7. Refit the tip ejector (refer to Changing the Tip Ejector for Multichannel Models on page 15).
Autoclaving
The upper part (body) and the piston assembly of the pipette are not autoclavable. Only the following parts may be
autoclaved: tip ejector, tip holder, and connecting nut. The O-ring and seal are not autoclavable. They may be cleaned or
replaced with the ones found in SPARE PARTS on page 18.
1. Clean the parts to be autoclaved, especially the tip holder.
2. Put the parts in an autoclaving sack.
3. Autoclave for 20 minutes at 121°C, 1 bar relative pressure.
4. Check that the parts are dry before reassembling the pipette.
5. Set the pipette aside to equilibrate to room temperature.
6. Reassemble the pipette (refer to Figure 11 on page 14).

CLEANING AND DECONTAMINATION
17
PIPETMAN® |USER’S GUIDE
Chemical Decontamination
You may choose to decontaminate your pipette chemically, in accordance with your own procedures. Whatever decontaminant
you use, check with the supplier of the decontaminant to verify that it is compatible with stainless steel and the plastics used in
the construction of the pipette: PA (Polyamide), PBT (Polybutylene Terephtalate), PC (Polycarbonate), PC/PBT (Polycarbonate/
Polybutylene Terephtalate), POM (Polyoxymethylene), PVDF (Polyvinylidene Fluoride), or PP (Polypropylene).
Chemical Decontamination for Single Channel Models
Upper Part (handle)
1. Wipe the upper part (handle) of the pipette with a soft cloth or lint-free tissue soaked with the chosen
decontaminant.
2. Wipe the upper part of the pipette with a soft cloth or lint-free tissue soaked with distilled water or sterile water.
Lower Part (volumetric module)
Only the following components can be immersed in a decontaminant solution: connecting nut, tip ejector, and tip holder.
NOTE The piston assembly and seals must be degreased with isopropanol or ethanol in a separate vessel before
being immersed in decontamination solution.
1. Remove the tip ejector, the tip holder, and the connecting nut.
2. Immerse them in the decontamination solution.
3. Degrease the piston assembly and the seals. Then immerse them in another vessel of decontamination solution.
4. Rinse each component with distilled water.
5. Let the parts dry by evaporation. The tip ejector, tip holder, and connecting nut can be wiped dry with a soft cloth.
6. Lubricate the piston assembly and the seals.
7. Reassemble the piston assembly, the tip holder, and the tip ejector.
Chemical Decontamination for Multichannel Models
Only the following components be immersed in a decontamination solution: tip ejector, ejector locks, and ejector spacer.
1. Remove the tip ejector and the ejector spacer.
2. Immerse the tip ejector, ejector locks and ejector spacer in the decontamination solution or wipe them with a soft cloth
or lint-free tissue soaked with the decontamination solution.
3. Rinse the components with distilled water.
4. Wipe the entire pipette with a soft cloth or lint-free tissue soaked with the decontamination solution.
5. Wipe the entire pipette with distilled water.
6. Let the parts dry by evaporation or wipe them with a clean soft cloth or lint-free tissue.
7. Refit the tip ejector (refer to Changing the Tip Ejector for Multichannel Models, on page 15).

SPARE PARTS
18 SPARE PARTS | PIPETMAN®
Service Kit 1st level includes:
●3 piston seals* or
seal guides
●3 O-rings*
●1 tip holder
Service Kit 2nd level includes:
●1 push button
●1 connecting nut
●Stainless steel
tip ejector F2
●Plastic tip ejector F1
(also available separately as
an accessory)
For the P2 and P10 models only:
●1 dual-position adapter for
stainless steel tip ejector
●1 extension for plastic
tip ejector
F3
Figure 13
Spare parts identified
C
A
B
D
E
F4
* For P5000, two piston seals and O-rings are provided in the Service Kit 1st level.
Chapter 8
SPARE PARTS
F3
A
B
C
D
E
F1 F2
F2
F4 F3

SPARE PARTS
19
PIPETMAN® |USER’S GUIDE
P2 (F144054M) AND P10 (F144055M)
LABEL DESCRIPTION P/N FOR
P2 P/N FOR P10
C+D+E Service Kit 1st level F144501 FA07001
A+B+F1 to F4 Service Kit 2nd level F1619702 F1619712
C+D Seal Guide + O-ring
(5 sets) F144861 FA07012
F3 Dual-Position Adapter
(x5) F144879 F144879
F4 Tip ejector extension F2070903 F2070903
P20 (F144056M) AND P100 (F144057M)
LABEL DESCRIPTION P/N FOR
P20 P/N FOR P100
C+D+E Service Kit 1st level FA07002 FA07003
A+B+F1+F2 Service Kit 2nd level F1619722 F1619732
C+D Seal Guide + O-ring
(5 sets) FA07013 FA07014
LABE
P200 (F144058M) AND P1000 (F144059M)
LABEL DESCRIPTION P/N FOR
P200
P/N FOR
P1000
C+D+E Service Kit 1st level FA07004 FA07005
A+B+F1+F2 Service Kit 2nd level F1619742 F1619782
C+D Seal Guide + O-ring
(5 sets) FA07015 FA07016
P5000 (F144066) AND P10ML (F144067)
LABEL DESCRIPTION P/N FOR
P5000
P/N FOR
P10ML
C+D+E Service Kit 1st level FA07311 FA07312
C+D Seal Guide + O-ring
(2 sets) FA07307 FA07308
E Tip holder FA07350 F161263
DESCRIPTION PART NUMBER
Lubricant 5440011070
Single Channel Models Multichannel Models
Tip ejector
(12 channels) Ejector lock
Ejector spacer
(8 channels)
F3
G
H1
Ejector spacer
(12 channels)
H2
Ejector spacer D10
(8 channels)
H3
Ejector spacer D10
(12 channels)
H4
F4
Tip ejector
(8 channels)
P8X10 (F144068) AND P12X10(F144069)
LABEL DESCRIPTION P/N FOR
P8X10
P/N FOR
P12X10
F3-F4 Tip ejector F507005 F507006
G Ejector lock F507008 F507008
H1 - H2 Ejector spacer F507001 F507003
H3 - H4 Ejector spacer D10 F807114 F807115
P8X20 (F144070) AND P12X20(F144071)
LABEL DESCRIPTION P/N FOR
P8X20
P/N FOR
P12X20
F3-F4 Tip ejector F507005 F507006
G Ejector lock F507008 F507008
H1 - H2 Ejector spacer F507001 F507003
P8X200 (F144072) AND P12X200(F144073)
LABEL DESCRIPTION P/N FOR
P8X200
P/N FOR
P12X200
F3-F4 Tip ejector F507005 F507006
G Ejector lock F507008 F507008
H1 - H2 Ejector spacer F507001 F507003
P8X300 (F144074) AND P12X300(F144075)
LABEL DESCRIPTION P/N FOR
P8X300
P/N FOR
P12X300
F3-F4 Tip ejector F507005 F507006
G Ejector lock F507008 F507008
H1-H2 Ejector spacer F507001 F507003

WARRANTY
20 WARRANTY | PIPETMAN®
Chapter 9
WARRANTY
Gilson warrants this pipette against defects in material under normal use and service for a period of three years for
both single channel and multichannel models from the date of purchase.
This warranty shall not apply to pipettes which are subject to abnormal use and/or improper or inadequate maintenance
(contrary to the recommendations given in the user’s guide), including, but not limited to pipettes which have been
subjected to physical damage, improper handling, or spillage or exposure to any corrosive environment. This warranty
shall also be void in the event pipettes are altered or modified by any party other than Gilson or its designates. Gilson’s
sole liability under this warranty shall be limited to, at Gilson’s sole option, repair or replacement of any defective
components of pipettes or refund of the purchase price paid for such pipettes. Routine cleaning, control, and
recalibration are not covered under the warranty. The replacement of wearing parts such as seals, O-rings, broken
pistons assembly, and broken tip holders are not covered under the warranty.
NOTICE
Yearly routine maintenance is highly recommended to keep your pipette in good condition, ensuring a
continued high level of performance.
THE FOREGOING WARRANTY IS EXCLUSIVE AND GILSON HEREBY DISCLAIMS ALL OTHER WARRANTIES, WHETHER
EXPRESS OR IMPLIED, INCLUDING ANY WARRANTIES OF MERCHANTABILITY AND ANY WARRANTIES OF FITNESS FOR A
PARTICULAR PURPOSE, UNDER NO CIRCUMSTANCES SHALL GILSON BE LIABLE FOR ANY CONSEQUENTIAL, PUNITIVE,
INDIRECT OR INCIDENTAL DAMAGES ARISING OUT OF ANY BREACH OF ANY EXPRESS OR IMPLIED WARRANTY.