Zeiss ULTRAPHOT II User manual

I ULTRAPHOT II

ULTRAPHOT II
INSTRUCTIONS

Conte nts
Description
The Comero
The Microscope
The Iliuminoting
Apporatus
Instrument Tobie
General Operating Instructions
Attaching the
seporately pocked ports
Adiusting the light sources
Checking the automatic exposure device
Calibration the
automatic exposure device
Working with Transmitted Light
Compound Microscope
Simple Microscope
Working with Incident Light
Compound Microscope
Simple Microscope
Photographing with the 9x 12 cm Comero
ULTRAPHO T l ias Polorising Microscope
Fluorescence Microscopy
Maintenance and Handling
Poge
3
7
20
31
37
40
42
51
56
58
61
62
66
72
77
81
85
89
91
92
-

Description
A
H
K
B
E
1
The Camero Microscope ULTRAPHOT II consists of various components which in port ore inter-
changeable. This results in on exceptional versatility. The component ports of Ihe apparatus ore
( fig . 1):
A Stond
G Sloge group with substage for transmit!-
B 9x 12 om. (4 XS"j Photo Head,
inter- ed light
changeable with H Illuminator
for low-voltage filament bulb
C
35 mm. PhOTO Head, or
12 V 100 W, or for high-pressure mercury
D Projection Head
lamp HBO 200
E Tube Head, interchangeable with
F luminor Head
(for low-power photo- K Corbon Arc
lamp
graphs) l Instrument Tobie 3

A Patf! of RGJI in the Compound Microscope
2
T rcIftIMitted 11 .....lnation
Incident Illumincrtion

1----~
2----
3----
,----
5----
6 - ----
7 ____ _
8----- ~
21
22
23
"'--- 2<1

32
4
25---!.---
26---- -
27- -- -
4
1 Attachment device for the Photo Heads
2 Mirror carriage
3 Clamping strip for the Photo Heads
-4 Locking pin
5 Attachment device for the Tube Heads
6 Stop for the Tube Heads
7 Clamping screw for the Tube Heads
8 Cover for the light exit
in epi-illumination
9 Clamping strip for sloge and condenser
carriers
10 Filter holder
11 Main switch of the automotic exposure
device
12 Pilot lamp, lights up when main swilen is
turned on
13 Pilot lamp, lights up 0$ long as shutter is
electrically opened
28
29
30
31

14 Push button for electric hand control
15 Push button for automatic control of the ca-
mero shutter
16 Metal cover for carrier of Tube Head , stage
and substage
17 Knob for adjusting extension of the camero
18 Carrier for Tube Head, stage and substage
19 Se}ting knob for illuminators
~} Ring dovetails, holders for the illuminators
22 Bose
23 Switching lever for changing from illumina-
tion for Compound Microscope (position
·Micro·) to illumination for LUMINARS
(position ·luminar"")
24 Operating disk for the diaphragm (Lumi-
nous-field diaphragm at "Micro", aperture
diaphragm at "Luminor")
25 lever for clamping the Photo Heads
26 Provision for disengagement of the mirror
carriage
27 Plug board for electric connection between
stand and Photo Head
28 Guiding edge for the Photo Head
29 Fastening screw of retaining angle bar (A 30)
30 Retaining angle bar for supporting the mir-
ror carriage during transportation
31 Fastening screw of (A 30)
32 Rings for adiusting the scoles of magnifica-
tion
33 Red reference points for Optovar positions
1.25, l.6,
and 2
A Stand (Fig. 4)
The Carrier (A 18), mainly mode of cost iron
ports, is rigidly mounted to the box-shaped Bose
(A 22). This rests with four shock-reducing rub-
ber feet on the surface supporting the instru-
ment. It contains (] port of the illuminating
system for transmitted light (Fig. 2 and 3). The
Carrier is portly enclosed by a metal cover (A
16). In the upper port of it s interior it contains
the camero
space and in the lower the remain-
der of the ports for transillumination 0$ well as
the system for epi-iliumination. On the outside
of the metal cover, at its lower end, are three
ring dovetails (A 20 and A 21), for attadlment
of the light sources; the third, opposite (A 21),
is not visible in the illustration.
In addition, the Carrier and metal cover corry
mounting devices
(A 1) for the Photo Heads
(A 5) for the Tube- or luminar Heads, and
(A 9) on the pinion box: for stages and con-
densers for transillumination 5

6
Table T
Magnifi
cat ion' on the focusing screen (B 7) wi th the Compo un d M ic r o,c op .
Votid for ULTRAPHOT II 0' of ",iol No. SS Ot6 {excepting 6·ploce numbers).
3000.
Objective 4 6.3 10 16 25
40 63 100
The table shows the magnifications obtainable with the combination of individual ob jectives
and t he three positions of the OPTOVAR (E 18).
Foetor 1.25 ;:: colored field
Foetor 1.6 = hatched field
Foetor 2
;:: block field

The Camera
The upper part of the interior of the carrier
with metal caver constitutes the camera. The
image
is projected, by means of twa mirrors
(Fig. 2 and 3) upon the focusing screen (8 7)
mounted in the front wall of the instrument
above the Tube Head. The camero length is
changed by horizontally displacing t he mirrors.
Bath of them rest
an a mirror carriage (A 2)
which can be adjusted by a knob (A 17) extend·
ing laterally from the caver wall. Through this
adjustment the camero length can be doubled.
An advantage of the arrangement is that with
relatively small dimensions a considerable
variation
in camero length is achieved. The
range with the Compound Microscope is 50 to
SO cm., for survey photographs with the Simple
Microscope
SO to 110 cm.
On the shaft of knob (A 17) for setting the
camera length are placed interchangeable rings
(A 32) which bear marks for the rapid setting
of specific magnifications. The respective marks
must lie
in contact with the lower edge of the
window, like the third ring
(A 32), in Fig. 4. There
are three such rings for the Compound Micros·
cape. They are distinguished by blade: numbers.
There
is one for each of the three positions of
the
OPTOVAR (E 18). The relation is given by 1,
2, or 3 red dots (A 33) which correspond to the
dots appearing under the factor numbers of the
OPTOVAR (E 20).
The numbers engraved on the rings indicate the
factor by which the objective magnification
must
be multiplied in order to obtain the magni·
ficotion on the focusing screen (8 7).
Exompl.:
Plonoduomot
4OJO.6S i. employed. The OPTOVAR i, '01
on 1 .6 12 Ad don). After f<KUling IIIe iMOge on It..
fix",jng 'etMn, the comero lodor IS i, indieotocl by tho
oo".,po<\ding ring. Totol mognjlicotion on 1110 f<Ku.ing
screon 4Ox15x =
6OOx.
A separate ri ng is provided for each of the five
LUMINARS employed for low power photo·
graphs (Simple Microscope). The correlation
between ring
and LUMINAR is indicated by the
some color of the engraving. The numbers en·
graved an the rings directly indicate the magni·
ficotion an the focusing screen (Table 11, page
67). An additional ring with millimeter graduo.
tian is provided for cases in which such an un·
equivocal correlation cannot be determined
beforehand. With this scale
an optional table
of magnifications can
be set up. 7

B •
•

•
9 x 12 em. (4 x 5") Photo Head
Retaining knob for the light-excluding hood
(here removed)
of the focusing screen. The
hood is sel on this knob and pressed down-
wards
2 Frome of the focusing screen holding the
ground-gloss plale
3 Retaining clip of the focusing screen frome
-4 Bolt for attaching (I roll film or sheet film
holder or (I ~Po l aroid holder·
5 Dry cartridge with silica gel. If Ihe contents
ore no longer blue, the cartridge is unscrew-
ed and dried on (I moderately warm heat-
ina pl ate until the dark blue color is restored
6 Se lector knob for Ihe sensi tivity of the em-
ployed emu ls ion. The longesl e)(posures are
obta ined with position -'-, Ihe shortest with
position -8"
7 Foc using screen with clear diogonal strips
8 Shutter opening
9 lever fo r opening a nd cocking the fo ca l,
plane shutter by h and. For cocking a nd clos·
ing the shuHer by h an d (p. 56, section 7) or
by insert
in g the pl ate hol der (p. 86, sectians
10 and 11) the knob fo r mech an ical rel ease
of the shutter (8 10) must not be in the
depressed position.
10 Knob for mechanical release of the shutter.
When pressed halfway in, the knob ca tches,
the shutter remains
open; pressed complete·
Iy in, the knob posses the catch and the
shutler remains open for
as long as the knob
is pressed
11 Release knob for the lock of the winding
lever
operated by the plate or fi lm holder.
This lock arrests the lever when a f
il m pack
or ro ll film magazine or a Polaroid holder
is used
12 Contact face of the Photo H ead
13 Window of yellow glass; behind it lies the
photocell for automatic control
of e)(POSUre
14 Terminal strip for electric connection bet·
ween Photo H ead and st and
15 Shutter housing 9

B 9x12 em. Photo Heod (for USA 4xS")
The Photo Head is otloched to the upper end
of the instrument.
It carries the devices for
focusing the image
and for receiving the hol-
den containing the film material. In its bosic
form
it is designed for 9x12 cm. or 4x5" hol-
ders. The change from one size to the other is
mode by transposing the guide bars (page 52).
The interior of the Photo Head houses the
essential port of the device for automatic
exposure control
and the shutter mechanism.
After taking off the Tube Head (E) and after
loosening clamping lever
(A 25), the Photo Head
can be removed from the instrument by pulling
it upwards. In its place a simple Praiection Head
(Fig. 8) or a 35 mm . Photo Head ( Fig. 6) can be
10 inserted.
In taking a photograph, the image is focused
on the focusing screen
(8 7) located immediate-
ly above the Tube Head. II is a ground-gloss
plate provided with diagonal clear strips. The
focusing screen need
nat be removed from the
instrument when inserting a plate or film holder.
The holder
is introduced from above between
housing
and frame (82) of the focusing screen.
In so doing Ihe frame springs back so thot the
plane
of the photographic emulsion lies in the
plane occupied by the ground glass during
focusing. When introduced the holder depresses
a lever
(334, page 53) which automatica ll y closes
and cocks the shutler.
This necessarily must be
open while focusing the image. The orrange-

,
,
•
ment ovoids errors in manipulation, svch as
leaving the shutter open when removing the
holder, or double exposures.
The holder should
be introduced gently to avoid
shod: which could alter the plane of focus. lever
(8 9) at the right of the focusing screen serves
for opening and cod:ing the shutter by hand.
Knob (8 10) at the right and below the focusing
screen
is a hand release of the shutter for time
elCposures. When the knob
is pushed halfway
in, to the catch, the shutler remains open, but
when pressed completely
in and released, the
shutter closes.
In the interior of the Photo Head, to the left of
the focusing screen,
is a photocell (8 13). It
determines the brightness of the imoge throvgh
o window, closed by a yellow filter, facing the
camero space. The correct exposure
is auta-
maticolly adiusted in occardonce with the reac-
tion of the phatocell by on electronic control
device located portly
in the Photo Head beside
the phatocell, ond portly in the interior of the
bose.
A prerequisite, naturally,
is correct odiustment
of the automatic device
to the sensitivity of the
film material employed. The procedure is de-
scribed on page 58 ond carried out with the aid
of knob
(8 6) having a scale for 8 sensitivity
steps.
11

c .
15
12
-'t--- 13
6
16
17
18

3S mm. Photo Head
Knob for mechanical shutter release
The shuller stays
open as l ang as the knob
is depressed; in that position it can be locked
by giving
it a half tu rn . The shutter remains
open until the lock is released,
2 Sase plate
3 Knob for adjusting the shuller speed. It is
set on -S" for automatic expasures, To
change the setting, th e kn ob is pulled out
to a stop
and turned un til the desired shutler
speed lies beside the mark into which the
knob
snaps upon release
4 Knob for changing between
clear disk with
cross lines
and gro und-gloss disk
5 Dry cartridge wi th silica ge l
6 Synchronizing contact for flash exposures
7 Centering screw for projective (should
no t
be manipulated by the user)
B Projective
9 light-excluding sleeve in front of the focus·
ing eyepiece
10 Eyecup
11 Screw for clamping the focusing eyepiece
(C 14) in the correct position for the observ-
er's eye
12 Some as (C 7)
13 Housing
14 focusing eyepiece wi thout light·excluding
sleeve. The eyepiece has a very high exit
pupil
in order to facilitate observation when
passing from observation through the bi·
noculor tube. The light-excluding sleeve (C 9)
should be attached if difficu lt y is experienc.
ed
in locating the exit pupi l.
15 Green marks on cossette and ring, to lie
opposite
one another when inserting the
cossette
16 Handle for the film winding mechanism
17 Cosselle
16 Fi lm counting device. After inserting the cos·
selle, set the number disk on 35 and make
two blank exposures by pressing knob (A 14)
or (C 1)
19 lever for opening the li ght-excluding flop
installed
in the observation eyepiece fo r the
purpose of preventing the
entrance of stray
light, during on exposure, through the eye·
piece, It is opened if long exposures require
checking of the focu
s. As for as possible t his
should be done with attached light-exclu d·
ing sleeve
20 Selector knob for sensitivity of the
fi lm
molerial
13

c
14
C 35 mm. Photo Head
ULTRAPHOT II is designed primarily for phot-
ography with relatively large film sizes. How-
ever, frequent ly it is desirable olso to use 35
mm. film. This can be accomplished by means
ofthewe ll -known Attachment Comera equipped
for the use
of 35 m m. film. Afler releasing
screw
(E 1 3), th e cover (E 12) can be removed
TobIe 2
Magnification with
the 35 mm . Photo Head
from Ihe Tube H ead (E 1). A vertical lube is
inserted in its place, carrying in i ts lower end
lens 473094. An Attochment Comero is mount-
ed on the tube, and photographs con be loken
on 35 mm. film as with ony compound micr os-
cope. Directions ore given in Ihe instructions
fo r the Attachment Comero (G 40-415).
Pr oiective 3.2x
with OPTOVAR in position
Ob iective
1.25 x 1.6x 2x
total factor total factor totol factor
'x
5x 6.3x
1
4X
5 x 6.3x
2.5 lOx
12.5x
16
x
4
16x
20 x 25
x
6.3
25x
32
x
40 x
10
40x
50
x
63
x
16
63x 80
x
100
x
25
l00x
125
x
160
x
..
160x
200
x 250 x
63
250 x I 320
x
400
x
100
400x
500
x 630 x

I
,
The requirements for 35 mm. photomicrography
ore met in a more complete manner by the
35
mm. Photo Head (f ig, 6). It is equipped with
automatic exposure device and automatic film
advance. This instrument is' mounted in place
of the 9x12 em. (4X5"j Photo Head on the
corrier of ULTRAPHOT II. Before doing so, the
cover
(E 12) is removed from the Tube Head (E
1 ). The Projective (e 8), attached in a centering
moun! at the lower end of the 35 mm. Photo
H ead, extends into the opening of the Tube
Head.
The intermediate (air) image formed by the com-
bination of microscope objective and OPIO·
VAR is projected by the Projective on Ihe film
01 a fur ther magnification of 3.2x. The total
magnifications obtained with the various com-
binations ore shown in toble 2.
Switch on the photoelectric cell diaphragm by
setting the ring
oround the drying car tridge
(C 5) to position ~1 ~ which should usually re-
main switched in at position ~2~. Only if cali-
brotion
photographs show that the change of
the exposure obtainable with the selector switch
(C 20) is too coarse by the foetor 2, inter-
mediate steps are possible by switching to
position ","_ This may occasionally be desir-
able for colour photogrophs . With the selector
switch
(C 20) in the some position the .x po s ur.
6_ will be increased by the foctor 1.5 app,OL
The 35 mm. Photo Head can be used only in
conjunction with the Tube Head (E 1 ). II co n·
not be used with the luminar Head (Fl)
for taking low-power photographs.
A beam-splitting system (Fig. 7) is mounted
above the Projective (C 8). II sends approxi-
mately't. of the light to the film, 'I. to the pha.
tocell, and the remainder to the focusing disk.
The focusing eyepiece
(C 14) serves for observa-
tion of the focusing disk 01 on increased magni-
fication. Before its use, after loosening screw
(C 11), the focus of the eyepiece is first adjusted
so that the
plane of the disk appears sharp to
the eye
of the observer. The exit pupil lies very
high, so that it is possible to easily pass from
ob .servalion through the inclined binocular tube
of the microscope to focusing of the image in
the focusing eyepiece. If there is difficulty in lo-
cating the exit pupil, the light-excluding sleeve
(C 9) should be attached. A clear-gloss and a
ground-gloss disk
are provided for focusing.
One or the other is brought into the path of
rays by moving knob (C 4) up and down.
The
film is contained in a drum·shaped cossette
(C 17) and introduced into the housing of the
35 mm. Photo Head so Ihot the 9, .. n line on
the knurled ring
of the cossette lies beside Ihe
gr •• n mark (C 15). By turning right to a stop
the cosselle
is locked in position, opened, and
coupled wilh the mechanism for automatic film
advance. Lever (C 16) serves for winding the
spring
of this mechanism.
A focal-plane shutter
is employed. Shutter
speeds for mechanical
release are adjusted
with knob
(e 3). To change the speed, the knob
is pulled out to a stop, turned until the desired
number lies beside the
fi>(ed mark, and then
allowed to snap in.
For hand operation the shutter is released
either by pressing the white knob
(A 14) on the
bose
of the instrument or knob (e 1) of the
camero. For time eKposures the shutter
is set on
B, for instantaneous shots on the time selected
(10 = ';'. sec., 25 = '/usec.,SO =' /50 sec., 100 ==
'/01>0 sec.).
If 35 mm. photomicrographs are to be mode
with automatic control of exposure, shutter knob
(e 3) is set on B and selector knob (e 20) on the
film sensitivity. Correct adjustment of the selec-
tor knob can not be mode on the basis of
the film sensitivity supplied by the manufacturer.
Instead, before beginning work with the appa-
ratus, it is necessary to make a series of lest
exposures
(page 58) on the film which is to be
employed. 15

o
16

, Hood
2 Scole for reoding the ongle
of rotolion
3 Hondle for rototing the projection screen
-4 Clomping screw for the projection screen
5 Housing
6 Fastening pin for the hood
7 Clomping screw
for fostening the projection
screen to the housing
Projection Head
The Projection Head consists essentially of a
housing (0 5) which serves as holder for a pro-
jection screen.
Th is rests in 0 (ototing mount
hoving a scole on
its periphery (0 2). If ongle$
ore to be measured with the device, it is a
simple matter for the user to drow suitoble lines
on the projection screen with a shorp hord lead
pencil.
The size of the imoge on the projection screen,
having a diameter of 190 mm., is the some as on
the focusing screen (8 7) of the 9x 12 cm. Photo
Head. Hence the magnifications given in toble 1
(poge
6), 11 (page 67), and 12 (poge 75) olso
opply to the Projection Head.
To colculote the diameter of the moximum
object
area which can be imoged at a given
mognificotion on the projection screen,
it is
necessory only to divide its diameter of 190 mm.
by the mognificotion. 17