Leica CM1950 User manual

Leica CM1950
Cryostat
Instructions for Use
English
Order No.: 14 0477 80101 - Revision N
Always keep this manual with the instrument.
Read carefully before working with the instrument.
Version 2.1, Revision N - 09/2019

3
Leica CM1950
The information, numerical data, notes and value judgments contained in this Instructions for Use
represent the current state of scientific knowledge and state-of-the-art technology as we understand it
following thorough investigation in this field.
We are under no obligation to update the present Instructions for Use periodically and on an ongoing
basis according to the latest technical developments, nor to provide our customers with additional
copies, updates etc. of this Instructions for Use.
To the extent permitted in accordance with the national legal system as applicable in each individual
case, we shall not be held liable for erroneous statements, drawings, technical illustrations etc.
contained in this Instructions for Use. In particular, no liability whatsoever is accepted for any financial
loss or other consequential damage caused by or related to compliance with statements or other
information in this Instructions for Use.
Statements, drawings, illustrations and other information regarding the contents or technical details of
the present Instructions for Use are not to be considered warranted characteristics of our products.
These are determined only by the contract provisions agreed between ourselves and our customers.
Leica reserves the right to change technical specifications as well as manufacturing processes without
prior notice. Only in this way is it possible to continuously improve the technology and manufacturing
techniques used in our products.
This document is protected under copyright laws. All copyrights to this documentation are held by
Leica Biosystems Nussloch GmbH.
Any reproduction of text and illustrations (or of any parts thereof) by means of print, photocopy,
microfiche, web cam or other methods – including any electronic systems and media – requires express
prior permission in writing by Leica Biosystems Nussloch GmbH.
For the instrument serial number and year of manufacture, please refer to the nameplate on the back of
the instrument.
Note
Leica Biosystems Nussloch GmbH
Heidelberger Strasse 17 - 19
69226 Nussloch
Germany
Tel.: +49 - (0) 6224 - 143 0
Fax: +49 - (0) 6224 - 143 268
Web: www.LeicaBiosystems.com

4Version 2.1, Revision N
Table of Contents
1. Important Information ................................................................................................................................ 6
1.1 Symbols in the text and their meanings....................................................................................................... 6
1.2 Instrument type................................................................................................................................................ 7
1.3 Qualification of personnel.............................................................................................................................. 7
1.4 Intended use of the instrument ..................................................................................................................... 7
2. Safety and Design....................................................................................................................................... 9
2.1 Safety notes...................................................................................................................................................... 9
2.2 Warnings........................................................................................................................................................... 9
2.3 General safety notes..................................................................................................................................... 10
2.4 Unpacking and installation........................................................................................................................... 10
2.5 Safety devices................................................................................................................................................ 13
2.6 Securing/locking the handwheel................................................................................................................ 14
2.7 Cleaning, disinfection – Turning the instrument back on ....................................................................... 15
2.8 Handling specimens – Defrosting............................................................................................................... 16
2.9 Removing the microtome.............................................................................................................................. 16
2.10 Maintenance .................................................................................................................................................. 16
3. Technical Data .......................................................................................................................................... 17
4. Standard delivery...................................................................................................................................... 21
5. General Overview..................................................................................................................................... 24
5.1 Control panel fields and cryostat chamber............................................................................................... 25
6. Installation ................................................................................................................................................. 26
6.1 Site requirements .......................................................................................................................................... 26
6.2 Transport to the site...................................................................................................................................... 26
6.3 Installing the handwheel.............................................................................................................................. 28
6.3.1 Locking/unlocking the handwheel.............................................................................................................. 29
6.3.2 Installing the footswitch dummy (instruments with cutting motor) ...................................................... 30
6.4 Electrical connection.................................................................................................................................... 30
6.5 Installing accessories/inserting chamber accessories.......................................................................... 31
6.5.1 Installing the adjustable footrest (optional) .............................................................................................. 31
6.5.2 Installing the storage systems (optional)................................................................................................... 32
6.5.3 Shelf, movable (optional).............................................................................................................................. 32
6.5.4 Inserting the section waste tray ................................................................................................................. 33
6.5.5 Installing the heat extractor, stationary (optional) .................................................................................. 33
6.5.6 Installing the knife/blade holder and adjusting the clearance angle ................................................... 34
6.5.7 Inserting/changing the bacteria filter ........................................................................................................ 35
6.5.8 Assembling the filter bag.............................................................................................................................. 35
6.5.9 Installing the section extraction (optional) – Use with blade holder CE only...................................... 36
7. Instrument Controls .................................................................................................................................. 38
7.1 Control panels on the Leica CM1950.......................................................................................................... 38
7.1.1 Control panel 1 ............................................................................................................................................... 38
7.1.2 Control panel 2 – Electric coarse feed, sectioning and trimming thickness ....................................... 40
7.1.3 Control panel 3 – Motorized sectioning (optional)................................................................................... 42

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Leica CM1950
Table of Contents
8. Working with the Instrument.................................................................................................................. 45
8.1 Preparing cutting tools, specimen discs and preparation aids............................................................. 45
8.2 Switching on the instrument........................................................................................................................ 45
8.3 Configuring the parameters......................................................................................................................... 46
8.4 Working with the precooled cryostat......................................................................................................... 50
8.4.1 Preparatory work........................................................................................................................................... 50
8.4.2 Trimming with extraction – 1. Anti-roll guide installed............................................................................ 53
8.4.3 Cutting with extraction – Anti-roll guide installed ................................................................................... 55
9. Troubleshooting ........................................................................................................................................ 57
9.1 Problems during work................................................................................................................................... 57
10. Temperature selection chart .................................................................................................................. 60
11. Optional Accessories............................................................................................................................... 61
11.1 Ordering information..................................................................................................................................... 61
12. Maintenance and Cleaning..................................................................................................................... 79
12.1 General maintenance instructions ............................................................................................................. 79
12.2 Changing fuses............................................................................................................................................... 80
12.3 Replacing the UVC lamp............................................................................................................................... 80
12.4 UVC lamp ordering information................................................................................................................... 84
12.5 Replacement of LED illumination ................................................................................................................ 85
13. Decontamination Confirmation.............................................................................................................. 86
14. Warranty and service............................................................................................................................... 87

6Version 2.1, Revision N
Important Information
1
1. Important Information
1.1 Symbols in the text and their meanings
Symbol: Title of the symbol: Warning
Description: Warnings appear in a white box and are marked by a
warning triangle.
Symbol: Title of the symbol: Note
Description: Notes, i. e. important user information, appear in a
white box and are marked by an information symbol.
Symbol: Title of the symbol: Caution – UVC radiation!
Symbol: Title of the symbol: Flammable freezing sprays prohibited
Description: This symbol alerts the user that the use of flammable
freezing sprays in the cryostat chamber is prohibited
due to explosion hazard.
Symbol: Title of the symbol: Item number
→"Fig. 7-1"Description: Item numbers for numbering illustrations. Numbers in
red refer to item numbers in illustrations.
Symbol: Title of the symbol: CE Compliance
Description: The CE marking is the manufacturer's declaration
that the medical product meets the requirements of
the applicable EC directives.
Symbol: Title of the symbol: Serial number
Description: Indicates the manufacturer's serial number so that a
specific medical device can be identified.
Symbol: Title of the symbol: Article number
Description: Indicates the manufacturer's catalog number so that
the medical device can be identified.
Symbol: Title of the symbol: Consult Instructions for Use
Description: Indicates the need for the user to consult the
Instructions for Use.
Symbol: Title of the symbol: Manufacturer
Description: Indicates the manufacturer of the medical product.

7
Leica CM1950
Important Information 1
Symbol: Title of the symbol: Manufacturing date
Description: Indicates the date when the medical device was
manufactured.
Symbol: Title of the symbol: In vitro diagnostic medical device
Description: Indicates a medical device that is intended to be
used as an in vitro diagnostic medical device.
Symbol: Title of the symbol: WEEE Symbol
Description: The WEEE symbol, indicating separate collection
for WEEE – Waste of electrical and electronic
equipment, consists of the crossed-out wheeled bin
(§ 7 ElektroG).
Symbol: Title of the symbol: China ROHS
Description: Environmental protection symbol of the China RoHS
directive. The number in the symbol indicates the
"Environmentally-friendly Use Period" of the product
in years. The symbol is used if a substance restricted
in China is used in excess of the maximum permitted
limit.
Symbol: Title of the symbol: CSA Statement (Canada/USA)
Description: The CSA test mark means that a product has
been tested and fulfills the applicable safety
and/or performance standards, including the
relevant standards defined or administered by the
American National Standards Institute (ANSI),
Underwriters Laboratories (UL), the Canadian
Standards Association (CSA), the National Sanitation
Foundation International (NSF) and others.
1.2 Instrument type
All information given in these Instructions for Use applies only to the instrument type indicated on
the title page. A nameplate indicating the instrument serial number is attached to the rear side of the
instrument. Precise data for the various versions is specified in (→p. 17 – 3. Technical Data).

8Version 2.1, Revision N
Important Information
1
1.3 Qualification of personnel
The Leica CM1950 may be operated by trained laboratory personnel only. The instrument is intended for
professional use only.
Prior to operating the instrument, the operator must thoroughly read and understand these Instructions
for Use and must familiarize him/herself with all technical details of the instrument.
Note
Despite chemical and/or UVC-light disinfection, personal safety precautions as per the applicable
laboratory regulations must still be taken (i.e. safety goggles, gloves, laboratory coat and mask must
be worn).
This type of disinfection reduces the number of germs by at least 99.99 %.
1.4 Intended use of the instrument
The Leica CM1950 is a high-performance cryostat with an encapsulated microtome and separate
specimen cooling. It features a UVC disinfection system, an (optional) integrated extraction system for
section waste and an (optional) motor for motorized sectioning.
The cryostat is designed to produce frozen sections for biological, medical and industrial applications.
The Leica CM1950 is suitable for in-vitro diagnostic (IVD) applications.
The instrument may only be operated within the scope of its designated use as described above and as
per the instructions given in these Instructions for Use.
Any other use of this instrument is considered as improper operation.

9
Leica CM1950
Safety and Design 2
2. Safety and Design
Warning
The safety and caution notes in this chapter must be observed at all times.
Be sure to read these notes even if you are already familiar with the operation and use of other Leica
products.
2.1 Safety notes
These Instructions for Use include important instructions and information related to the operating safety
and maintenance of the instrument.
The Instructions for Use are an important part of the product, and must be read carefully prior to startup
and use and must always be kept near the instrument.
This instrument has been built and tested in accordance with the safety requirements for electrical
equipment for measurement, control, and laboratory use.
To maintain this condition and ensure safe operation, the user must observe all notes and warnings
contained in these Instructions for Use.
Note
For the CE certificate and up-to-date certificates pertaining to UVC disinfection, visit us online at:
www.LeicaBiosystems.com.
Note
These Instructions for Use must be appropriately supplemented as required by the existing
regulations on accident prevention and environmental safety in the operator‘s country.
Warning
• The protective devices located on the instrument and the accessories must not be removed or
modified. The instrument must only be opened and repaired by service technicians authorized by
Leica.
• Only original spare parts and permitted original accessories may be used!
• Use only one of the power cords approved by Leica. This must not be replaced with a different
power cord! If the power plug does not fit in the socket, then contact our service.
2.2 Warnings
The safety devices installed in this instrument by the manufacturer only constitute the basis for accident
prevention. Operating the instrument safely is, above all, the responsibility of the owner, as well as the
designated personnel who operate, service or repair the instrument.
To ensure trouble-free operation of the instrument, make sure to comply with the following instructions
and warnings.

10 Version 2.1, Revision N
Safety and Design
2
Note
The instrument has been designed and constructed with the latest state-of-the-art technology and
according to recognized standards and regulations with regard to safety technology. Operating or
handling the instrument incorrectly can place the user or other personnel at risk of injury or can
cause damage to the instrument or other property. The instrument may be used only as intended and
only if all of its safety features are in proper working condition. Malfunctions that impede safety must
be remedied immediately.
2.3 General safety notes
The Leica CM1950 is a cryostat with an encapsulated microtome and separate specimen cooling. It is
primarily used for work in the area of fast-cut diagnostics.
The displays and instrument controls are easy to operate due to their largely self-explanatory symbols.
LED displays make it easy to read. The cryochamber is made of seamlessly welded, high-quality
stainless steel that is free of difficult-to-access corners and thus easy to clean and disinfect.
Warning
Due to explosion hazard, the use of flammable freezing sprays inside the cryostat chamber, while the
instrument is turned on, is prohibited.
Note
Flammable substances may not be used in the Leica CM1950 when it is turned on and plugged in.
Do not place staining solutions or other liquids on top of the instrument.
2.4 Unpacking and installation
To ensure proper function of the instrument, it must be set up with a minimum distance on all sides from
walls and furniture (→p. 26 – 6.1 Site requirements)).
• The instrument may only be transported in an upright or slightly inclined position.
• To ensure a safe transport with a forklift 3 people are required: one operating the forklift, and the
other 2 holding the instrument on either side to prevent it from sliding down.
• Before connecting to the power supply system, please observe (→p. 17 – 3. Technical Data).
• Never connect the instrument to a power socket that does not have a protective conductor terminal.
Length of the power cord: up to 3.5 m extension possible: NO
Note
After transporting, wait at least 4 hours before turning the instrument on. This waiting period is
necessary to allow the compressor oil, which may have been displaced during transport, to return
into its original location. Any condensation on electrical parts that has formed due to temperature
differences during transport must be allowed to dry completely. Failure to comply with this can cause
severe damage to the instrument!

11
Leica CM1950
Safety and Design 2
Note
• When the instrument is delivered, check the tilt indicators on the packaging.
• If the arrowhead is blue, the shipment was transported laying flat, was tilted at too
great an angle or fell over during transport. Note this on the shipping documents
and check the shipment for possible damage.
• Two persons are required to unpack the instrument!
• Instrument figures and pictures are only to be used as examples to explain the
unpacking procedure.
Fig. 1
1
2
1. To remove the strips (→Fig. 1-1), suitable
shears and protective gloves are required.
2. Stand next to the crate and cut the strips at the
location shown (see arrows in (→Fig. 1)).
3. Lift the outer carton (→Fig. 1-2)upwards and
out.
Warning
Caution when removing the strips! There is a
risk of injury (the strip has sharp edges and
is under tension)!
Fig. 2
8
4
4
6
3
5
7
4. Carefully remove the adhesive tape
(→Fig. 2-3)holding the two transport anchors
(→Fig. 2-4)on both sides of the instrument
and remove them.
5. Pull the dust cover (→Fig. 2-5)from the
instrument.
6. Remove the two white and the two blue
transport anchors (→Fig. 2-6), which protect
the chamber window.
7. Remove all accessories (→Fig. 2-7).

12 Version 2.1, Revision N
Safety and Design
2
Fig. 3
9
8. Lift and remove the wooden edge (→Fig. 3-9).
9. Remove the ramp (→Fig. 4-13)from the pallet.
10. Properly insert the ramp. Make sure that the
ramp components designated "L" (left) and
"R" (right) click into place in the intended
guide channel (→Fig. 4-14). When correctly
assembled, the guide rails (→Fig. 4-16)are
located inside, while the arrows (→Fig. 4-15)
point toward each other.
Fig. 4
13
10
11
12
14
15
16
Warning
• Do not push the device by its hood (→Fig. 4-10)!
• Instead, use transport grip points •(→Fig. 4-11)!
• The front and rear rollers (→Fig. 4-12) must stay on the ramp (→Fig. 4-13). Danger of tipping!
11. Carefully roll the instrument backwards over the ramp from the pallet.
12. Push the instrument to the installation location on the castors (→Fig. 4-12).

13
Leica CM1950
Safety and Design 2
2.5 Safety devices
The Instructions for Use include important instructions and information related to the operating safety
and maintenance of the instrument.
These Instructions for Use are an important part of the product, and must be read carefully BEFORE
startup and use and must always be kept near the instrument.
If additional requirements on accident prevention and environmental protection apply in the country
of operation, these Instructions for Use must be supplemented by appropriate instructions to ensure
compliance with such requirements.
The instrument is equipped with the following safety devices: an emergency stop switch (motorized
instruments only), a handwheel lock and centering system (motorized instruments only), safety guard on
the blade and knife holder, and a blade ejector.
Warning
To prevent adverse health effects from UVC radiation, the UVC disinfection cycle can be started only
after the sliding window has been properly closed. Closing the window activates the corresponding
safety features.
The consistent use of these safety features and strict observation of the warnings and cautions in these
Instructions for Use will safeguard the operator from accidents and/or personal injury to a great extent.
Microtome knives
• Take care when handling microtome knives/disposable blades. The cutting edge is extremely sharp
and can cause serious injuries!
• Never leave knives and knife holders with a knife/blade mounted lying around!
• Never place a knife on a table with the cutting edge facing upward!
Note
We strongly recommend using the safety gloves included with the standard delivery.
• NEVER try to catch a falling knife!
• Before handling the specimen or the knife, or changing the specimen, lock the handwheel and ensure
that the knife is covered by the safety guard.
• Avoid contact with cold parts of the instrument as this can cause freezer burn – wear the safety
gloves supplied!

14 Version 2.1, Revision N
Safety and Design
2
Safety guard
Warning
Prior to making modifications to the knife and specimen, changing the specimen or knife, or taking a
break, always lock the handwheel and cover the cutting edge with the safety guard!
The CE, CN and CN-Z knife holders feature safety guards; the glass anti-roll plate of the CE knife holder
also serves as a safety guard.
2.6 Securing/locking the handwheel
Warning
Always lock the handwheel prior to making modifications to the knife or specimen, changing the
specimen, or taking a break!
12 o'clock
position
6 o'clock
position
Fig. 5
1
2
Fig. 6
To lock the handwheel, press the lever (→Fig. 6-1)outward. Continue turning
the handwheel slowly until the grip is in the upper or lower position and the
handwheel is locked. Press the lever fully outward; gently rock the handwheel
back and forth until the locking mechanism clicks into place noticeably.
To release the handwheel, press the lever (→Fig. 6-2)on the handwheel
toward the cryostat housing.
Centering of the handwheel (motorized instruments only)
Fig. 7
Pull out the handwheel's handle and position it in the middle of the
handwheel. The handle automatically engages in this position.

15
Leica CM1950
Safety and Design 2
Note
An important safety device on the cryostat is the centering of the handwheel on motorized
instruments.
Warning
Rotate the handwheel only if the refrigeration system is on and the cryochamber is cold.
2.7 Cleaning, disinfection – Turning the instrument back on
Note
It is not necessary to remove the microtome for disinfection.
• The instrument has been designed for UVC disinfection.
Note
Remove section waste after EVERY sectioning operation and BEFORE changing specimens. Remove
the section waste using the extraction nozzle (optional) or by using a paper towel soaked in an
alcohol based disinfectant. Do not start the disinfection before swiveling the anti-roll plate to the side.
Each new specimen is a potential source of contamination.
• When disinfecting the instrument, take appropriate protective measures (gloves, mask, protective
clothing, etc.).
• When using detergents and disinfectants please comply with the safety precautions of the
disinfectant manufacturer!
• The integrated glass anti-roll guide of the blade holders CE, CN and CN-Z can be cleaned either with
acetone or alcohol.
• Dispose of waste liquid according to the waste disposal regulations.
• Do not use external heaters for drying the cryochamber. This can cause damage to the cooling
system!
• Do not turn the instrument on before the cryochamber is completely dry. Frost formation!
• All components removed from the cold cryostat must be carefully dried before returning them to the
cryochamber!
• The front panel and the slit cover of the microtome must be completely dry before turning on the
instrument!
Note
For more detailed information about disinfection, visit the Leica Biosystems Division website at
www.LeicaBiosystems.com

16 Version 2.1, Revision N
Safety and Design
2
2.8 Handling specimens – Defrosting
• When working with contaminated or infected material, the general safety guidelines for laboratories
must be applied!
• Before defrosting the cryochamber remove all samples!
• Before defrosting the specimen head, remove all samples!
Note
Never leave samples in the cryochamber! – The instrument is not suitable for storing frozen
specimens, as the refrigeration dehydrates the specimens!
Warning
The quick freeze shelf can become very hot during the defrosting process. Therefore, do not touch it!
2.9 Removing the microtome
• The microtome is encapsulated and therefore does not require removal by the user.
2.10 Maintenance
Replacing the fuses
• Turn the instrument off and disconnect the power plug before replacing the fuses!
• Use only the fuse types specified in (→p. 17 – 3. Technical Data)! The use of fuses other than
those specified by the manufacturer may cause severe damage to the instrument!
Replacement of the UVC lamp
• Turn the instrument off and disconnect the power plug before replacing the UVC lamp.
Warning
It is possible to break the UVC lamp during replacement. If this happens, the lamp change must be
completed by Technical Service. If any metallic mercury is released, handle it carefully and dispose
of it properly.
Note
If both disinfection indicator lights are blinking alternately, the UVC lamp must be
replaced!

17
Leica CM1950
Technical Data 3
3. Technical Data
Note
All specifications related to temperature are valid only up to an ambient temperature of 18 °C to 35 °C
and a relative humidity of no more than 60 %!
Instrument type -1 -2 -3
Model numbers 14047742460,
14047742461,
14047742463,
14047742462
14047742464,
14047742465,
14047742466,
14047742467,
14047743909,
14047743908,
14047743907,
14047743906,
14047744626
14047742456,
14047742457,
14047742458,
140477442459,
14047743905,
14047743904,
1404774625
Nominal voltage (±10 %) 100 VAC 120 VAC 230 VAC
Nominal frequency 50/60 Hz 60 Hz 50 Hz
Power consumption (with/
without object head cooling)
1500/1300 VA 1500/1300 VA 1500/1300 VA
Power supply IEC 60320-1 C-20 IEC 60320 C-20 IEC 60320-1 C-20
Mains input fuse (automatic
circuit breaker)
T15 A M3 T15 A T1 T10 A T1
Other fuses F1 T4A 250 VAC (5x20 heater - object head)
F2 T4A 250 VAC (5x20 sectioning motor)
F3 T4A 5x20 250 VAC (5x20 power supply 5V and 24V electronics,
keyboards,valves, slaveboard, feed)
F4 T4A 250 VAC (5x20 heater - drip pan)
F5 T4A 250 VAC (5x20 heaters - window, grid and drain tube)
F6 T0.630A 250 VAC (5x20 illumination and UVC disinfection)
F7 T2A 250 VAC (5x20 peltier element)
F8 T2A 250 VAC (5x20 clutch)
F9 T1.6A 250 VAC (5x20 extraction)
Dimensions and weights
Overall size of device, without
handwheel (width x depth x
height)
700 x 850 x 1215 mm
Overall size of device, with
handwheel (width x depth x
height)
835 x 850 x 1215 mm
Working height (armrest) 1025 mm
Overall size of packging (width x
depth x height)
960 x 820 x 1420 mm

18 Version 2.1, Revision N
Technical Data
3
Dimensions and weights
Empty weight (without
accessories)
145-193 kg depending on
configuration
Environmental specification
Operating altitude 1Max. 2000 m above sea level
Operating temperature +18 °C to +35 °C
Relative humidity (operation) 20 to 60 % RH non-condensing
Transport temperature -29 °C to +50 °C
Storage temperature +5 °C to +50 °C
Relative humidity
(transport / storage)
10 to 85 % RH non-condensing
Minimum distance to walls Back: 150 mm
Right: 300 mm
Left: 150 mm
Emissions and boundary conditions
Overvoltage category 1II
Pollution degree 12
Means of protection 1Class I (PE connected)
Degree of protection according
to IEC 60529
IP20
Heat emission (max., with /
without object head cooling)
1500/1300 J/s
A-weighted noise level,
measured at 1 m distance
< 70 dB (A)
EMC class A (FCC Rules part 15)
A (CISPR 11, IEC 61326, CAN ICES-3 (A)/NMB
Warning
Observe item (→p. 26 – 6.1 Site requirements)!
Refrigeration system
Cryochamber
Temperature range 0 °C to -35 °C ±5 K, adjustable in 1 K increments, at ambient
temperature of 20 °C
Cooling time to -25 °C at starting
point and ambient temperature of
20 °C
approx. 5 h
Cooling time to -35 °C at starting
point and ambient temperature of
20 °C
approx. 8 h
Max. operating pressure 25 bar

19
Leica CM1950
Technical Data 3
Refrigeration system
Refrigerant* 320 g, refrigerant R-452A*
Defrosting Hot gas defrosting
Manual defrosting Yes
Automatic defrosting
Programmable Yes (hot gas defrost), selectable time
Defrosting intervals 1 defrost in 24h or manual hot gas defrost
Defrost time 12 minutes
Automatic shutoff defrost At chamber temperature of more than -5 °C
Object head cooling
Optional feature included in
model number
At 230 V/50 Hz: 14047742456, 14047742457, 14047742458,
14047742459
At 120 V/60 Hz: 14047742464, 14047742465, 14047742466,
14047742467
At 100 V/50 Hz and 60 Hz: 14047742460, 14047742461, 14047742462,
14047742463
Temperature range -10 to -50 °C ± 3 K
Refrigerant and quantity At 230 V/50 Hz: 137 g, refrigerant R-452A*
At 120 V/60 Hz: 147 g, refrigerant R-452A*
At 100 V/50/60 Hz: 147 g, refrigerant R-452A*
Max. operating pressure 25 bar
Defrosting object head cooling
Defrosting Electrical heater
Automatic defrosting No
Manual defrosting Yes
Defrost duration 15 min.
Defrost temperature 45 °C ±2K
Quick-freeze shelf
Minimum temperature -42 °C (±5 K), at chamber temp. -35 °C (+5 K)
Number of freezing stations 15+2
Defrosting Manual hot gas defrost
Peltier element
Max. temperature difference -17 K, at chamber temperature of -35 °C +5 K
Number of freezing stations 2
1in acc. with IEC-61010; UL 3101
2for additional details, see (→p. 41 – Setting section/trim thickness)
3in acc. with CECOMAF: Liquid temperature 45 °C, evaporation temperature: -25 °C
Warning
* Refrigerant and compressor oil must be replaced by qualified, authorized service personnel only!

20 Version 2.1, Revision N
Technical Data
3
Microtome
Type Rotary microtome, encapsulated
Section thickness range 1 to 100μm
Trimming thickness range Clinical: 10 to 40 μm
Research: 1 to 600 μm 2
Horizontal specimen feed 25 mm + 1 mm
Vertical specimen stroke 59 mm ± 0.5 mm
Specimen retraction 20 μm (can be deactivated)
Maximum specimen size 50 x 80 mm
Sectioning speed Slow: 0 to 50 strokes/min
Fast: 0 to 85 strokes/min
Max. speed: 85 to 90 strokes/min
Specimen orientation ±8 ° (x-, y-axis)
Coarse feed Slow: 300 μm/s
Fast: 900 μm/s
UVC disinfection
Manual start Yes
Automatic start No
Manual abort Yes
Duration desinfection cycle Short cycle: 30 min
Long cycle: 180 min