Leica CM1510S User manual

Instruction manual
Leica CM1510 S – Cryostat
V1.4 English – 04/2010
Always keep this manual near the instrument.
Read carefully prior to operating the instrument.
/HLFD&0 6
Cryostat

3
Leica CM1510 S – Cryostat
The information, numerical data, notes and
value judgments contained in this manual rep-
resent the current state of scientific knowl-
edge and state-of-the-art technology as we
understand it following thorough investigation
in this field.
We are under no obligation to update the
present manual according to the latest tech-
nical developments, nor to provide our cus-
tomers with additional copies, updates etc. of
this manual.
For erroneous statements, drawings, techni-
cal illustrations etc. contained in this manual
we exclude liability as far as permissible ac-
cording to the national legal system applicable
in each individual case. In particular, no liabili-
ty whatsoever is accepted for any financial
loss or consequential damage caused by or
related to compliance with statements or other
information in this manual.
Statements, drawings, illustrations and other
information as regards contents or technical
details of the present manual are not to be
considered as warranted characteristics of
our products.
These are determined only by the contract
provisions agreed between ourselves and our
customers.
Leica reserves the right to change technical
specifications as well as manufacturing pro-
cesses without prior notice. Only in this way is
it possible to continuously improve the tech-
nology and manufacturing techniques used in
our products.
This document is protected under copyright
laws. Any copyrights of this document are re-
tained by Leica Biosystems Nussloch GmbH.
Any reproduction of text and illustrations (or of
any parts thereof) by means of print, photo-
copy, microfiche, web cam or other methods –
including any electronic systems and media –
requires express prior permission in writing by
Leica Biosystems Nussloch GmbH.
For the instrument serial number and year of
manufacture, please refer to the name plate at
the back of the instrument.
© Leica Biosystems Nussloch GmbH
NOTE
Published by:
Leica Biosystems Nussloch GmbH
Heidelberger Str. 17 - 19
D-69226 Nussloch
Germany
Phone: [phone]
Fax: [phone]
eMail: [email protected]
Internet: http://www.leica-microsystems.com

4Instruction manual V1.4 – 04/2010
Table of contents
1. Important information ................................................................................................................ 6
1.1 Symbols used in the text and their meaning .......................................................................................... 6
1.2 Instrument type ............................................................................................................................................. 6
1.3 Designated use ............................................................................................................................................. 6
1.4 Qualification of personnel .......................................................................................................................... 6
2. Safety ............................................................................................................................................ 7
2.1 Safety instructions ....................................................................................................................................... 7
2.2 Dangers, warnings and cautions .............................................................................................................. 7
2.3 Safety features ............................................................................................................................................ 11
2.3.1 Locking the handwheel ............................................................................................................................. 11
2.3.2 Knife guard ................................................................................................................................................... 11
3. Instrument components and specifications ........................................................................ 12
3.1 Overall view - instrument parts ................................................................................................................ 12
3.2 Technical data ............................................................................................................................................ 14
4. Setup ........................................................................................................................................... 16
4.1 Installation site requirements .................................................................................................................. 16
4.2 Standard delivery - packing list ............................................................................................................... 17
4.3 Unpacking and installation ...................................................................................................................... 18
4.4 Fastening the handwheel ......................................................................................................................... 19
4.5 Electrical connection ................................................................................................................................20
4.6 Prior to operation ........................................................................................................................................ 20
5. Operation .................................................................................................................................... 22
5.1 Control panel ............................................................................................................................................... 22
5.2 Controller ...................................................................................................................................................... 23
5.3 Setting user-definable parameters......................................................................................................... 24
6. Daily work with the instrument ............................................................................................. 26
6.1 Specimen freezing ...................................................................................................................................... 26
6.1.1 Quick-freeze shelf....................................................................................................................................... 26
6.2 Inserting the specimen discs .................................................................................................................. 26
6.2.1 Fixing the specimen discs in the specimen head ............................................................................... 27
6.2.2 Specimen orientation ................................................................................................................................ 27
6.3 Inserting the knife or blade into the knife holder ............................................................................... 27
6.4 Sectioning / trimming ................................................................................................................................. 28
6.4.1 Moving the specimen with the coarse / precision feed .................................................................... 29
6.4.2 Section thickness setting ......................................................................................................................... 29
6.5 Defrosting ..................................................................................................................................................... 30
6.5.1 Automatic defrosting of the cryochamber ............................................................................................ 30
6.5.2 Manual defrosting of the cryochamber ................................................................................................ 30
6.5.3 Manual defrosting of the quick-freeze shelf ....................................................................................... 31

5
Leica CM1510 S – Cryostat
Table of contents
6.6 Finishing work .............................................................................................................................................. 31
6.6.1 Finishing the daily work ............................................................................................................................. 31
6.6.2 Shutdown for a longer period .................................................................................................................. 32
6.7 Temperature selection chart ................................................................................................................... 33
7. Troubleshooting ....................................................................................................................... 34
7.1 Possible causes and remedies ............................................................................................................ 34
7.2 Display error messages ......................................................................................................................... 38
7.3 Thermal circuit breaker (120V model only)....................................................................................... 38
8. Cleaning and maintenance .................................................................................................... 39
8.1 General maintenance instructions ..................................................................................................... 39
8.2 Automatic circuit breaker...................................................................................................................... 40
8.3 Cleaning ...................................................................................................................................................... 41
8.3.1 Spray disinfection with Leica Cryofect.............................................................................................. 41
8.4 Removing the microtome........................................................................................................................ 43
8.5 Removing the microtome housing ....................................................................................................... 44
8.6 Reinstalling the microtome .................................................................................................................... 45
8.7 Replacing the fuses ................................................................................................................................. 46
8.8 Replacing the lamp .................................................................................................................................. 47
9. Optional accessories .............................................................................................................. 48
9.1.1 Mobile heat extractor ............................................................................................................................. 48
9.1.2 Stationary heat extractor ...................................................................................................................... 48
9.1.3 Thermal block............................................................................................................................................ 49
10. Warranty and service.............................................................................................................. 53
11. Decontamination Certificate (master) ................................................................................... 54
12. Information for the People´s Republik of China ................................................................ 56

6Instruction manual V 1.4 – 04/2010
1.1 Symbols used in the text and their
meaning
Dangers, warnings and cautions ap-
pear in a gray box and are marked by
a warning triangle .
Notes, i.e. important user informa-
tion appear in a gray box and are
marked by an information symbol
.
Figures in brackets refer to item nos.
in drawings.
1.2 Instrument type
All information provided in this manual applies
only to the instrument type indicated on the title
page.
1. Important information
1.3 Designated use
The Leica CM1510S is a powerful cryostat for
routine and research applications in biology,
medicine and industry, where it serves for
quick-freezing and subsequent sectioning of
specimen material.
The instrument is not designed for unattended
storage of specimen material.
The instrument must be operated only ac-
cording to its designated use (see description
above) and according to the instructions con-
tained in this manual.
Any other use of the instrument will be con-
sidered improper.
1.4 Qualification of personnel
• Der Leica CM1510 S may be operated only
by trained laboratory personnel.
• All laboratory personnel designated to op-
erate the Leica CM1510 S must read this in-
struction manual carefully and must be fa-
miliar with all technical features of the
instrument before attempting to operate the
Leica CM1510 S.
(5)
A name plate indicating the serial number of
the instrument is attached to the back of the
instrument.
Fig. 1

7
Leica CM1510 S – Cryostat
Be sure to comply with the safety instructions and warnings in this chapter. Be sure to read
these instructions, even if you are already familiar with the operation and use of other
Leica products.
2. Safety
2.1 Safety instructions
This instruction manual contains important in-
structions and information regarding the oper-
ational safety and maintenance of the instru-
ment.
The instruction manual is an important part of
the product, which must be read carefully pri-
or to setup and use and must always be kept
near the instrument.
If additional requirements on acci-
dent prevention and environmental
protection exist in the country of op-
eration, this instruction manual must
be supplemented by appropriate in-
structions to ensure compliance
with such requirements.
This instrument was built and tested in accor-
dance with the safety regulations for electrical
measuring, control, regulating and laboratory
devices.
For current information about applicable stan-
dards, please refer to the CE declaration of
conformity on our Internet site:
www.leica-microsystems.com
In order to maintain this condition and ensure
safe operation, the operator must observe all
the instructions and warnings contained in
this instruction manual.
The protective devices on both instrument and accessories must neither be removed nor
modified.
Only authorized and qualified service personnel may repair the instrument and access the
instrument’s internal components.
2.2 Dangers, warnings and cautions
The safety devices installed in this instrument by the manufacturer only constitute the ba-
sis for accident prevention. Primarily responsible for accident-free operation is above all
the institution which owns the instrument and, in addition, the designated personnel who
operates, services or repairs the instrument. To ensure trouble-free operation of the in-
strument, be sure to comply with the following instructions and warnings.

8Instruction manual V1.4 – 04/2010
Warnings - Electrical connection
Please refer to and comply with the ‘Technical data‘!
The instrument must be connected to a grounded mains power outlet socket!
During the start-up of the compressor the nominal voltage must not drop below the values
specified in the ‘Technical Data‘ chapter!
The compressor requires a start-up current between 45 and 50 A.
Therefore, the electric circuit at the installation site must be inspected by a qualified elec-
trician to ensure that it meets the requirements for a smooth operation of the instrument.
For trouble-free operation, a constant power supply according to specification must be en-
sured at all times. Failure to comply with the above will cause damage to the instrument.
After transporting, wait at least 4 hours before switching the instrument on!
This waiting period is necessary to allow the compressor oil, which may have been dis-
placed during transport, to return to its original position.
Failure to comply with this can cause severe damage to the instrument.
2. Safety
Warnings - Transport and installation
The instrument must be transported either in an upright position or, if inclined, at an angle
of max. 30 degrees!
Do not operate in rooms with explosion hazard!
To ensure the instrument works trouble-free, the instrument must be set up with a least 10
cm distance from all walls and furniture.
Do not place anything next to the compressor ventilation grid (right side of the cabinet) to
ensure adequate ventilation!
The protective foam parts between microtome and compressor MUST be removed prior to
operating the instrument!

9
Leica CM1510 S – Cryostat
Warnings - sectioning
Be careful when handling microtome knives and disposable blades. The cutting edge is ex-
tremely sharp and can cause severe injury!
Never leave knives/blades or knife holders with a knife/blade mounted lying around!
Always store knives in the knife box when not in use!
Never place a knife anywhere with the cutting edge facing upwards!
NEVER try to catch a falling knife!
Always clamp the specimen before the knife!
Be careful when picking up the sections! The cutting edge is exposed!
When working inside the cryochamber, keep hands away from gilled evaporator – high risk
of injury from sharp edges!
Prior to manipulating knife and specimen, when changing the specimen or during breaks,
always lock the handwheel and cover the cutting edge with the knife guard!
Avoid skin contact with cold parts of the instrument as this can cause frostbite!
Cleaning and disinfection
Do not use organic solvents or any other aggressive substances for cleaning and disinfec-
tion! The instrument is suitable for spray disinfection with Leica Cryofect!
Only use alcohol or commercial disinfectants based on alcohol for disinfection!
For spray disinfection follow the instructions for use supplied with the disinfectant!
Do not use any external heaters for drying the cryochamber. This can cause severe damage
to the heating system!
2. Safety

10 Instruction manual V 1.4 – 04/2010
Removing the microtome from the cryochamber
Prior to removing the microtome turn off the instrument mains switch and pull the mains
plug.
Before removing the microtome, rotate the handwheel until the specimen cylinder is posi-
tioned in the lowest position. Otherwise, when removing the microtome, the specimen head
will rapidly fall down and might injure the operator’s hands!
Wear appropriate protective gloves to remove the cold microtome from the cryochamber.
Prolonged skin contact with cold parts of the instrument can cause frost bite!
The microtome must be completely dry before reinstallation. Humidity inside the microtome
will condense and freeze in the cold cryostat and thus may cause malfunctions or damage!
Warnings - maintenance
Only qualified and authorized service personnel may access the internal components of the
instrument for service and repair.
Clean the compressor ventilation grid at least once a year or more often if required!
Replacing the fuses:
Before replacing the fuses, turn off the instrument mains switch and pull the mains plug!
Only use replacement fuses of the same specification. For the required values, please refer
to chapter 3.2 ‘Technical Data‘!
Replacing the fluorescent light lamp:
Before replacing the lamp, turn off the instrument mains switch and pull the mains plug!
If the lamp is broken, it must be replaced by the technical service, as the replacement pro-
cedure involves a high risk of injury!
Do not use any lamp type other than the one inserted by the manufacturer!
2. Safety

11
Leica CM1510 S – Cryostat
2.3 Safety features
The instrument is equipped with the following safety features: hand-
wheel locking mechanism and knife guard on knife holder.
The consistent use of these safety features and strict observation of
the safety instructions and warnings in this manual will safeguard the
operator from accidents and/or personal injury to a great extent.
2.3.1 Locking the handwheel
To lock the handwheel, rotate the handwheel until the handle is in the
upper position and the metal pin (1) is located opposite the black dot (2).
Push the metal pin outward into the recess at the handwheel; if neces-
sary move the handwheel slightly back and forth, until you feel that the
locking mechanism engages.
To unlock the handwheel, push the metal pin (1) out of the recess at the
handwheel, towards the cryostat housing.
Prior to manipulating knife and specimen, when changing the
specimen or during breaks, always lock the handwheel and
cover the cutting edge with the knife guard!
2.3.2 Knife guard
Knife holder CN is equipped with a knife guard; on the knifeholder CE, the
glass plate of the anti-roll guide constitutes the knife guard (see sepa-
rate instruction manual for your knife holder).
2. Safety
21
Fig. 2

12 Instruction manual V 1.4 – 04/2010
3. Instrument components and specifications
3.1 Overall view - instrument parts
Fig. 3
Specimen
disc
Section
waste tray
Brush shelf
Knife holder CN
Knife holder base
Knife holder CE

13
Leica CM1510 S – Cryostat
Use the motorized coarse feed to rapidly move
the specimen head towards or away from the
knife.
Carefully observe the specimen
when moving it towards the cutting
edge to avoid an accidental colli-
sion of the specimen with the knife
edge, which can cause severe dam-
age to both knife and specimen.
3. Instrument components and specifications
Thermal
block
(optional
accessory)
Heat extractor
Quick-freezing shelf
Fig. 4
Control
panel
Motorized coarse feed
forward or
backward
- slow
forward
or backward
- fast
On/Off switch
for cryochamber
illumination
Display indicating:
- Set / actual temperature
- Real time
- Defrost time

14 Instruction manual V 1.4 – 04/2010
3.2 Technical data
Operating temperature range from +18 °C to +35 °C. All specifications related to temperature
are based on an ambient temperature of +22 °C and maximum air humidity of 60%!
Type CM1510 S CM1510 S CM1510 S
Nominal voltage 100 V AC ±10% 120 V AC ±10% 230 V AC ±10%
Nominal frequency 50/60 Hz 60 Hz 50 Hz
Power draw 2000 VA 1300 VA 1500 VA
Protective class I I I
Mains fuses Automatic circuit Automatic circuit Automatic circuit
breaker T 20 A breaker T 15 A breaker T 10 A
Pollution degree 2 2 2
Overvoltage inst. category II II II
Power draw 495 W / 850 W 850 W 495 W
Refrigerating output
at evaporator temperature 470 W / 780 W 780 W 470 W
of -25 °C
Nominal pressure 25 bar (maximum value)
Safety factor 3
Refrigerant 215 g ± 5 g R404A *
Compressor oil 0.6 l EMKARATE RL244b, ICI *
Refrigeration system 50 Hz 60 Hz
Temperature setting range 0 °C to -30 °C ±10%
at an ambient temperature of 22°C
and air humidity _<60 %
when sliding window is closed;
adjustable in 1-degree increments
Temperature of quick-freezing shelf Max. -44 °C
- at a temperature of -30 °C
Defrosting Automatic hot gas defrosting,
defrost time freely programmable,
manual defrosting of quick-freeze shelf and evaporator
duration: 8 min.
* Refrigerant and compressor oil must be replaced by authorized service personnel only!
3. Instrument components and specifications

15
Leica CM1510 S – Cryostat
3. Instrument components and specifications
Microtome
Rotary microtome
Section thickness setting 1 -60 µm; in 1-µm steps from 1 to 10 µm
in 2-µm steps from 10 to 20 µm
in 5-µm steps from 20 to 60 µm
Total horizontal specimen feed 25 mm
Vertical stroke 59 mm
Maximum specimen size 55 x 55 mm
Specimen orientation 8° (x-, y-, z-axis)
Electrical coarse feed
slow 0.2 µm/s
fast 0.7 µm/s
Lamp
50 Hz version: Osram Dulux S 11 W/21, light color LUMILUX bright white
60 Hz version: Osram Dulux S 13 W/21, light color LUMILUX bright white
Cryocabinet
Dimensions
Width (w/o handwheel) 880 mm
Width (with handwheel) 680 mm
Depth 680 mm
Height (arm rests) 1010 mm
Height (overall) 1175 mm
Weight (incl. microtome) approx. 135 kg
Please refer to chapter 4.1 - ‘Installa-
tion site requirements’!
All CM1510S instrument types require the following secondary fuses:
F4: T0.5 A 6.3 x 32 mm; Fa. Schurter, Typ FST; Fa. Littlefuse, Typ 313
F5: T1.0 A 6.3 x 32 mm; Fa. Schurter, Typ FST; Fa. Littlefuse, Typ 313
F6: T2.0 A 6.3 x 32 mm; Fa. Schurter, Typ FST; Fa. Littlefuse, Typ 313
F7: T4.0 A 6.3 x 32 mm; Fa. Schurter, Typ FST; Fa. Littlefuse, Typ 313
F8: T2.0 A 6.3 x 32 mm; Fa. Schurter, Typ FST; Fa. Littlefuse, Typ 313

16 Instruction manual V1.4 – 04/2010
4.1 Installation site requirements
Do not operate in rooms with explosion hazard!
To ensure the instrument works trouble-free, the instrument must be set up with at least 10
cm distance from all walls and furniture.
Do not place anything next to the compressor ventilation grid (right side of the cabinet) to
ensure adequate ventilation!
The place of installation must meet the following requirements:
• no direct sunlight,
• mains power socket at a distance no greater than 2.5 m (length of
power cord = 2.5 m) - no extension cords must be used!
• no drafts (air condition outlets etc.),
• even floor,
• virtually vibration-free floor,
• handwheel freely and easily accessible,
• room temperature maximum 35 °C, (< 22 °C recommended),
• relative humidity max. 60 %,
• only for indoor use.
High room temperature and excessive air humidity at the installation site affect the cooling
capacity of the cryostat.
4. Setup

17
Leica CM1510 S – Cryostat
4.2 Standard delivery - packing list
Order no.
1 Basic instrument .................................................................... 14 0436 38461
1 Stationary heat extractor ..................................................... 14 0369 11197
1 Handwheel with marking, antibacterial ........................... 14 0471 42558
1 Optional clamping lever for object head .......................... 14 0112 28806
1 Set of specimen discs ........................................................... 14 0470 43550
-4 Specimen discs, 25 mm .................................................... 14 0416 19275
-4 Specimen discs, 30 mm .................................................... 14 0370 08587
1 Adapter for Miles Tissue Tek specimen discs................ 14 0436 26747
1 Section waste tray ................................................................. 14 0471 30787
1 Storage shelf, right................................................................. 14 0471 30789
1 Storage shelf, left ................................................................... 14 0436 38503
1 Brush shelf............................................................................... 14 0398 13088
1 Tool set: .................................................................................... 14 0436 43463
-1 Brush, fine ........................................................................... 14 0183 28642
-1 “Leica” brush w/magnet................................................. 14 0183 40426
-1 Allen key, size 1.5 ............................................................... 14 0222 10050
-1 Allen key, size 2.5 ............................................................... 14 0222 04137
-1 Allen key, size 3.0 ............................................................... 14 0222 04138
-1 Allen key, size 4.0 ............................................................... 14 0222 04139
-1 Allen key with spherical head, size 4.0 ......................... 14 0222 32131
-1 Allen key, size 5.0 ............................................................... 14 0222 04140
-1 Allen key with handle, size 5.0 ........................................ 14 0194 04760
-1 Allen key, size 6.0 ............................................................... 14 0222 04141
-1 Single-head wrench, size 13 .......................................... 14 0330 33149
-1 Single-head wrench, size 16 .......................................... 14 0330 18595
1 Bottle of cryostat oil, type 407, 50 ml .................................. 14 0336 06098
1 Bottle of OCT-Compound, mounting medium
for cryosectioning, 125 ml .................................................... 14 0201 08926
1 Instruction manual Leica CM1510 S - G/E/F/S ................. 14 0436 80001
4. Setup

18 Instruction manual V 1.4 – 04/2010
4.3 Unpacking and installation
Unpacking as per the unpacking instructions attached to the outside of
the instrument shipping crate.
The instrument must be transported either in an upright position
or slightly inclined, at an angle of max. 30 degrees!
When pushing the instrument, do not grip the cabinet at the lid.
Grip the cabinet only at the marked areas ( )!
• Use the size 16 fork wrench (1) to com-
pletely screw in the adjustable feet.
• Move instrument to installation site on roll-
ers (2and 3).
• The adjustable feet (1) can be used to
slightly tilt the instrument (max. 30 degrees).
• To lift the instrument please use the handle
straps provided attaching them to the front
(2) and rear rollers (3).
• At the installation site, unscrew the adjust-
able feet (1) with the fork wrench to ensure
stability.
• Align the adjustable feet to level the instru-
ment.
After transporting, wait at least 4
hours before switching the instru-
ment on!
This waiting period is necessary to
allow the compressor oil, which may
have been displaced during trans-
port, to return to its original position.
Instrument
rear wall
231
4. Setup
Fig. 5

19
Leica CM1510 S – Cryostat
The handwheel and all hardware
items needed to attach it to the cry-
ostat are packed in the accessory
box. The handwheel can be dis-
mounted e.g. for transport through
narrow doors.
• Insert the pin (1) of the handwheel shaft
into the bore (2) of the handwheel.
• Place spring washer (3) onto screw (4) with
the curved surface pointing to the right side
(see also Fig. 6).
• Tighten screw (4) with the size 6 Allen key.
To disassemble, proceed in reverse order.
The protective foam parts between
microtome and compressor MUST be
removed prior to operating the instru-
ment (see Fig. 7)!
Caution! Edges of gilled evaporator
are extremely sharp! High risk of in-
jury!
4.4 Fastening the handwheel
1
2
34
Fig. 6
5
Fig. 7
4. Setup
The handwheel is lockable with the handle in
the upper position.

20 Instruction manual V 1.4 – 04/2010
4. Setup
4.5 Electrical connection
During the start-up of the compressor the nominal voltage must
not drop below the values specified in the ‘Technical Data‘!
The compressor requires a start-up current between 45 and 50 A.
Therefore, the electric circuit at the installation site must be in-
spected by a qualified electrician to ensure that it meets the re-
quirements for a smooth operation of the instrument.
For trouble-free operation, a constant power supply according to
specification must be ensured at all times.
Failure to comply with the above will cause damage to the in-
strument.
• Protect the electric circuit at the place of installation separately.
• Do not connect any other consumers to this electric circuit.
4.6 Prior to operation
• Verify if mains voltage and frequency in your laboratory comply with
the power rating indicated on the name plate of the instrument!
• Place the storage shelves into the chamber.
• Insert section waste tray (1) and brush shelf (2).
• Place the knife holder base on the microtome base plate and clamp.
• Mount the knife holder onto the knife holder base and clamp (see
separate instruction manual for your knife holder).
• Open the knife box with the knife and place the open box into the
cryochamber for precooling.
• Place all tools needed for specimen preparation into the
cryochamber.
• Close the sliding window.
• Connect the mains plug to the wall outlet socket.
After transporting, wait at least 4 hours before switching the in-
strument on!
This waiting period is necessary to allow the compressor oil,
which may have been displaced during transport, to return to its
original position.
Failure to comply with this can cause severe damage to the in-
strument.
1
2
Fig. 8