Leica ASP300 S User manual

Leica ASP300 S
Advanced Smart Processor
Automated vacuum
tissue processor
Operating Manual
Leica ASP300S
V1.0 English - 07/2006
Always keep this manual together with the instrument.
Read this instruction manual carefully before working
with the instrument.

3
Leica ASP300 S
The information, numerical data, notes and value
judgments contained in this manual represent
the current state of scientific knowledge and
state-of-the-art technology as we understand it
following thorough investigation in this field.
We are under no obligation to update the present
manual according to the latest technical devel-
opments, nor to provide our customers with addi-
tional copies, updates etc. of this manual.
For erroneous statements, drawings, technical
illustrations etc. contained in this manual we
exclude liability as far as permissible according
to the national legal system applicable in each
individual case. In particular, no liability whatso-
ever is accepted for any financial loss or conse-
quential damage caused by or related to compli-
ance with statements or other information in this
manual.
Statements, drawings, illustrations and other
information as regards contents or technical
details of the present manual are not to be
considered as warranted characteristics of our
products.
These are determined only by the contract provi-
sions agreed between ourselves and our cus-
tomers.
Leica reserves the right to change technical
specifications as well as manufacturing process-
es without prior notice. Only in this way is it pos-
sible to continuously improve the technology and
manufacturing techniques used in our products.
This document is protected under copyright
laws. Any copyrights of this document are
retained by Leica Microsystems Nussloch GmbH.
Any reproduction of text and illustrations (or of
any parts thereof) by means of print, photocopy,
microfiche, web cam or other methods – including
any electronic systems and media – requires
express prior permission in writing by
Leica Microsystems Nussloch GmbH.
For the instrument serial number and year of
manufacture, please refer to the name plate at
the back of the instrument.
© Leica Microsystems Nussloch GmbH
NOTE
Published by:
Leica Microsystems Nussloch GmbH
Heidelberger Str. 17 - 19
D-69226 Nussloch
Germany
Telephone: [phone]
Fax: [phone]
Internet: http://www.histo-solutions.de

4Operating Manual V 1.0 – 07/2006
Table of Contents
1. Important information ................................................................................................................. 6
2. Safety .............................................................................................................................................. 7
2.1 Safety instructions .................................................................................................................................... 7
2.2 Warnings .................................................................................................................................................... 7
2.3 Integrated safety devices ...................................................................................................................... 11
3. Instrument components and specifications ......................................................................... 12
3.1 Overview - instrument components..................................................................................................... 12
3.2 Specific instrument options .................................................................................................................. 14
3.3 Standard delivery - packing list ............................................................................................................ 15
3.4 Technical Data ........................................................................................................................................ 16
3.5 Compatible reagents .............................................................................................................................. 19
4. Setting up the instrument ......................................................................................................... 20
4.1 Site requirements ................................................................................................................................... 20
4.2 Electrical connection ............................................................................................................................. 20
4.3 Installing the accessories ..................................................................................................................... 22
4.4 Setting up the data connections .......................................................................................................... 23
4.5 Alarm functions ....................................................................................................................................... 24
4.6 Switching the instrument on ................................................................................................................. 25
4.7 Touchscreen functions .......................................................................................................................... 27
4.8 Checklist for first operation ................................................................................................................... 28
5. Operation ..................................................................................................................................... 29
5.1 Setting up the instrument parameters ................................................................................................. 29
5.1.1 System setup ........................................................................................................................................... 29
5.1.2 Access levels .......................................................................................................................................... 32
5.1.3 INSTALLATION menu ............................................................................................................................. 34
5.1.4 Editing the reagent list ........................................................................................................................... 37
5.1.5 Viewing the program list ........................................................................................................................ 40
5.1.6 Adding and/or modifying programs ..................................................................................................... 41
5.1.7 Favorites ................................................................................................................................................... 44
5.1.8 Editing the stations ................................................................................................................................. 46
5.1.9 Reagent groups ....................................................................................................................................... 47
5.2 Reagent handling .................................................................................................................................... 48
5.2.1 Fill/drain reagents (other than wax) ..................................................................................................... 48
5.2.2 Changing the wax ................................................................................................................................... 50

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Leica ASP300 S
Table of Contents
5.3 Running programs .................................................................................................................................. 53
5.3.1 Editing a selected program ................................................................................................................... 54
5.3.2 Starting a program .................................................................................................................................. 56
5.4 Reagent status ........................................................................................................................................ 57
5.5 System Monitor ....................................................................................................................................... 58
5.6 On-line help .............................................................................................................................................. 59
6. Solutions ...................................................................................................................................... 60
6.1 General ..................................................................................................................................................... 60
6.2 Power failure ........................................................................................................................................... 60
6.3 Troubleshooting ...................................................................................................................................... 61
6.4 Typical fill or drain problems................................................................................................................. 62
7. Cleaning and Maintenance ..................................................................................................... 63
7.1 Clean programs ....................................................................................................................................... 63
7.1.1 Retort clean programs ........................................................................................................................... 63
7.1.2 Wax clean program ................................................................................................................................ 65
7.1.3 Smart Clean ............................................................................................................................................. 66
7.2 General cleaning steps .......................................................................................................................... 67
7.2.1 Daily cleaning and maintenance .......................................................................................................... 69
7.2.2 Periodic cleaning and maintenance .................................................................................................... 71
7.3 Preventative maintenance schedule ................................................................................................... 72
8. Warranty and Service ............................................................................................................... 74

6Operating Manual V 1.0 – 07/2006
Symbols used in this manual
Warnings
appear in a gray box and are
marked by a warning triangle .
Notes,
i.e. important user information ap-
pear in a gray box and are marked
by an information symbol .
Flammable solvents and reagents
are marked with this symbol.
Instrument surfaces which become
hot during operation are marked
with this symbol.
Avoid direct contact with these sur-
faces - they may cause burns.
Figures in brackets refer to item
nos. in drawings.
Function keys to be pressed on the
instrument touch screen are written
in bold-print capital letters.
1. Important information
Designated use
The instrument has been designed so that it is
safe to use by the operator as well as for pro-
cessing specimens – provided that it is operated
according to the present instruction manual.
The Leica ASP300S is a modular automated
tissue processor designed for the following labo-
ratory applications:
• fixation,
• dehydration
• paraffin wax infiltration of histological
tissue specimens.
The Leica ASP300 S must be operated exclusively
with the reagents listed
in chapter 3.5 - "Compatible reagents" .
(5)
ENTER
Qualification of personnel
• The Leica ASP300 S may be operated only by
trained laboratory personnel.
•
The instrument may be operated only accord-
ing to the instructions contained in this manual.
Instrument type
All information provided in this manual applies
only to the instrument type indicated on the title
page.
Fig. 1
A name plate
indicating the
instrument
serial number
is attached to
the back of the
instrument.
Any other use of the instrument is
considered improper!
Failure to adhere to these instruc-
tions may result in an accident,
personal injury, damage to the
instrument or accessory equipment.

7
Leica ASP300 S
2. Safety
2.1 Safety instructions
This instruction manual includes important infor-
mation related to the operating safety and main-
tenance of the instrument and it is an important
part of the product.
If additional requirements on acci-
dent prevention and environmental
protection exist in the country of op-
eration, this instruction manual
must be supplemented by appropri-
ate instructions to ensure compli-
ance with such requirements.
Make sure to comply with the safety instructions and warnings in this chapter.
Make sure to read these instructions, even if you are already familiar with the operation
and use of other Leica products.
The protective devices on both instrument and accessories may neither be removed nor
modified. Only authorized and qualified service personnel may repair the instrument and
access the instrument’s internal components.
2.2 Warnings
The safety devices installed in this instrument by the manufacturer only constitute the basis for
accident prevention. Primarily responsible for accident-free operation is above all the institution
which owns the instrument and, in addition, the designated personnel who operates, services or
repairs the instrument.
To ensure trouble-free operation of the instrument, make sure to comply with the following
instructions and warnings.
This instrument has been built and tested in ac-
cordance with the safety regulations on electri-
cal measuring, control, regulating and laboratory
devices.
In order to maintain this condition and to ensure
safe operation the operator must comply with the
instructions and warning contained in this
instruction manual.
For current information on applicable
standards, please refer to the instru-
ment’s CE declaration and visit:
http://www.histo-solutions.com

8Operating Manual V 1.0 – 07/2006
2. Safety
Transport and Installation
The instrument may only be transported in an upright position.
Follow the unpacking instructions carefully to avoid damage to the instrument!
A cleaning must be performed prior to each transport, during which the instrument may
be shaken, tilted or lifted. Severe internal damage may otherwise occur.
The instrument MUST be connected to an earthed mains power outlet socket. The instru-
ment must not be connected to an extension cord without protective earth conductor.
Ensure uniform current supply according the to set voltage!
The set voltage CANNOT be changed by the user.
Severe damage may occur if the instrument is connected to a power supply voltage other
than that to which it was originally set.
The instrument must be set up in a well ventilated area, free from any ignition sources.
The chemicals to be used in the Leica ASP300 S are both flammable and noxious.
Do not operate the instrument in rooms with explosion hazard.
If there is a significant difference in temperature between the warehousing and the in-
stallation site of the instrument and if at the same time there is a high air humidity level,
condensation water may form. In this case, a waiting period of at least two hours must be
observed before the instrument is switched on. Failure to adhere to this waiting period
may result in damage to the instrument. Failure to adhere to this waiting period may re-
sult in damage to the instrument.
Warnings - Markings on the instrument itself
Markings on the instrument showing the warning triangle indicate that the correct
operating instructions (as defined in this manual) must be followed when operating or
replacing the item marked. Failure to adhere to these instructions may result in an acci-
dent, personal injury, damage to the instrument or accessory equipment.
Some instrument surfaces become hot during operation.
They are marked with this warning label. Touching these surfaces may cause
burns.

9
Leica ASP300 S
Warnings - Operating the instrument
The Leica ASP300S may only be operated by trained laboratory personnel, according to
its designated use and per the present instruction manual.
The ON/STOP switch at the side of the instrument may be used in an emergency to stop
the instrument during operation.
Always press PAUSE before opening the retort lid during processing to allow the retort to
vent.
Do not remove the wax drain hose or the remote fill/drain hose until the fill or drain has
been completed, as pressurized air is used to clear the hose after each fill / drain.
After refilling / replacing the reagent bottles ensure that the lids are tight.
The bottles must be properly pushed home into the connection manifolds at the rear inner
wall of the reagent module.
Failure to correctly plug the reagent bottles into the manifold will cause an interruption
to the processing run and may result in spilling of reagents.
Fixatives containing mercuric salts, acetic or picric acid will corrode metallic compo-
nents in the instrument.
After each paraffin step a retort clean cycle must be run.
2. Safety
Material safety data sheets can be obtained from the supplier of the chemicals.
They are also available on the Internet:
http://www.msdsonline.com

10 Operating Manual V 1.0 – 07/2006
Warnings -handling reagents
Be careful when handling solvents!
Always wear rubber gloves and safety goggles when handling the chemicals used in this
instrument.
Reagents used for tissue infiltration can be both toxic and/or flammable.
To avoid damaging the instrument only use those reagents that are listed in chapter 3.5!
Do not use acetone, benzene or trichlorethane in the instrument!
Use caution when handling paraffin wax or removing baskets – molten paraffin is hot and
may cause burns.
Also, avoid personal contact with paraffin stations and retort walls – they can be very hot
as well.
Dispose of waste solvents with care according to local regulations and the waste man-
agement policy of the company or institution.
Do not clean reagent bottles in an automatic dishwasher – they are NOT dishwasher-
proof.
2. Safety
Warnings - Cleaning and maintenance
Prior to each maintenance and/or cleaning, switch the instrument off and disconnect
mains power.
Do not clean the instrument with solvents containing acetone or xylene. No liquid may be
spilled into the internal components of the instrument – neither during operation nor
during cleaning.
When working with cleaning detergents, comply with all safety instructions by the manu-
facturer of the product and the laboratory management policy.
The condensate bottle should be inspected at least once every week and, if necessary,
emptied.