Leica TCS SP8 X User manual

10
Living up to Life
User Manual
Leica TCS SP8
Leica TCS SP8 MP
Leica TCS SP8 X

Published by:
Leica Microsystems CMS GmbH
Am Friedensplatz 3
D-68165 Mannheim (Germany)
http://www.leica-microsystems.com
http://www.confocal-microscopy.com
Responsible for contents: Leica Microsystems CMS GmbH
Copyright © Leica Microsystems CMS GmbH.
All rights reserved.

3
Copyright
Copyright
All rights to this document are held by Leica Microsystems CMS GmbH. Adaptation,
translation and reproduction of text or illustrations (in whole or in part) by print, photocopy,
microfilm or other method (including electronic systems) is not allowed without express
written permission from Leica Microsystems CMS GmbH.
Programs such as LAS and LAS AF are protected by copyright laws. All rights reserved.
Reproduction, adaptation or translation of these programs is prohibited without prior written
permission from Leica Microsystems CMS GmbH.
This User Manual specifies names of products or services that are trademarks or registered
trademarks of the respective trademark owners. Rather than including a trademark (TM or
®) symbol at every occurrence of a trademarked name, we state that we are using the
names only in an editorial fashion, and to the benefit of the trademark owner, with no
intention of infringement.
Made in Germany.
© Copyright Leica Microsystems CMS GmbH.
All rights reserved.

4
Copyright

5
Contents
Contents
Copyright. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3
Contents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5
1 About this User Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11
2 Intended Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13
3 Liability and Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15
3.1 Important Information for Operators and Users. . . . . . . . . . . . . . . . . . . . . . . . . . . . .15
4 Meaning of the warning messages in the manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17
5 General Safety Notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19
5.1 Commissioning and Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19
5.2 Modifications to the System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19
5.3 Safety Devices and Safety Labels. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20
5.4 Laser Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20
5.5 Electrical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21
5.6 Contact with Liquids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .22
5.7 Malfunction of the System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .22
6 Additional Notes on Handling the System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23
6.1 Location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23
6.2 Using the Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23
6.3 Protecting the System. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .24
6.3.1 Objectives . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .24
7 System Overview and Properties. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25
7.1 TCS SP8 System Variants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25
7.1.1 TCS SP8 with upright microscope and compact supply unit . . . . . . . . . . . . . . .25
7.1.2 TCS SP8 with upright microscope and flexible supply unit. . . . . . . . . . . . . . . . .27
7.1.3 TCS SP8 with inverted microscope and compact supply unit . . . . . . . . . . . . . .28
7.1.4 TCS SP8 with inverted microscope and flexible supply unit. . . . . . . . . . . . . . . .29
7.2 TCS SP8 MP System Variants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .30
7.2.1 TCS SP8 MP with DM6000 and IR laser without Dispersion Compensation . .30
7.2.2 TCS SP8 MP with DM6000 and IR Laser with Dispersion Compensation. . . . .31
7.2.3 TCS SP8 MP with DMI6000 and IR Laser without Dispersion Compensation .32
7.2.4 TCS SP8 MP with DMI6000 and IR Laser with Dispersion Compensation . . . .33
7.3 TCS SP8 X System Variants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34

6
Contents
7.3.1 TCS SP8 X with upright microscope . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34
7.3.2 TCS SP8 X with inverted microscope . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35
7.4 TCS SP8 with external UV laser. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .36
7.5 Controls on the Supply Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .36
7.5.1 Main switch board on the flexible supply unit . . . . . . . . . . . . . . . . . . . . . . . . . . .36
7.5.2 Control panel field on the compact supply unit. . . . . . . . . . . . . . . . . . . . . . . . . . .37
7.6 Technical Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37
7.6.1 Dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37
7.6.2 Weight of the supply unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38
7.6.3 Electrical specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38
7.7 Electromagnetic Compatibility. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38
7.8 Serial Number. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39
8 Ambient Conditions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41
8.1 General Requirements Regarding Ambient Conditions. . . . . . . . . . . . . . . . . . . . . . .41
8.2 Vibrations. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41
8.3 Room Dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .42
8.4 Electrical Connection Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43
8.4.1 TCS SP8 with flexible supply unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43
8.4.2 TCS SP8 with compact supply unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43
8.4.3 TCS SP8 MP with flexible supply unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44
8.4.4 TCS SP8 MP with compact supply unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44
8.4.5 TCS SP8 X . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44
8.4.6 External Lasers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45
8.5 Load capacity of the multiple socket outlet on the flexible supply unit . . . . . . . . .45
8.6 Waste Heat and Cooling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46
8.6.1 TCS SP8 with flexible supply unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46
8.6.2 TCS SP8 with compact supply unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .47
8.6.3 TCS SP8 MP with flexible supply unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .47
8.6.4 TCS SP8 MP with compact supply unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .47
8.6.5 TCS SP8 X . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .48
8.6.6 External Lasers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .48
9 Laser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .49
9.1 Laser Classes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .49
9.2 Overview of usable lasers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .49
9.2.1 VIS/UV Lasers for TCS SP8. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .49
9.2.2 VIS/UV Lasers for TCS SP8 X . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .50
9.2.3 IR lasers for TCS SP8 MP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .50
9.2.3.1 Picosecond laser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .50

7
Contents
9.2.3.2 Femtosecond laser. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .51
10 Safety Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .53
10.1 Main Circuit Breaker for Disconnecting the Power Supply . . . . . . . . . . . . . . . . . . .53
10.1.1 Compact Supply Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .53
10.1.2 Flexible Supply Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .53
10.2 Key Switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .54
10.2.1 Master Key Switch on the Compact Supply Unit . . . . . . . . . . . . . . . . . . . . . . . . .54
10.2.2 Master Key Switch on the Flexible Supply Unit . . . . . . . . . . . . . . . . . . . . . . . . . .54
10.2.3 Key Switch for the White Light Laser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .55
10.2.4 Key Switch for UV Lasers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .55
10.2.5 Key Switches for Other External Lasers. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .56
10.3 Emission Warning Indicators. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .56
10.3.1 Emission Warning Indicator on the Supply Unit . . . . . . . . . . . . . . . . . . . . . . . . . .56
10.3.2 Emission Warning Indicator at the White Light Laser . . . . . . . . . . . . . . . . . . . . .57
10.3.3 Emission Warning Indicator on UV Lasers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57
10.3.4 Emission Warning Indicator on Other External Lasers . . . . . . . . . . . . . . . . . . . .58
10.3.5 Malfunction of Emission Warning Indicator . . . . . . . . . . . . . . . . . . . . . . . . . . . . .58
10.4 Interlock Connectors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .58
10.4.1 Interlock Connector on the Supply Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .58
10.4.2 Interlock Connector on the White Light Laser . . . . . . . . . . . . . . . . . . . . . . . . . . .60
10.4.3 Interlock Connector on Other External Lasers . . . . . . . . . . . . . . . . . . . . . . . . . . .61
10.4.4 Interlock Connector on the Scan Head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .61
10.5 Safety Switches on the Microscope . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .62
10.6 Warning Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .62
10.7 Special Laser Safety Equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .63
10.7.1 Laser Protection Tube and Laser Protection Shield . . . . . . . . . . . . . . . . . . . . . .63
10.7.2 Safety Beam Guide on the TCS SP8 MP. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .64
11 Safety Labels on the System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .65
11.1 Inverted microscope . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .66
11.2 Upright Microscope. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .68
11.3 Scan Head. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .70
11.4 Compact Supply Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .72
11.5 Flexible Supply Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .72
11.6 MP Beam Coupling Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .73
11.7 Cover for replacement flange . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .74
11.8 Mirror Housing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .75
11.9 White light laser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .76

8
Contents
11.10 External UV Laser. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .76
11.11 Other External Lasers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .77
12 Switching On the System. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .79
12.1 TCS SP8/SP8 X with flexible supply unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .79
12.2 TCS SP8 with compact supply unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .84
12.3 HyD Reflected Light Detectors (HyD RLDs) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .88
13 LAS AF . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .91
13.1 Starting LAS AF . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .91
13.2 Structure of the Graphical User Interface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .92
13.3 LAS AF Online Help . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .95
13.3.1 Structure of Online Help . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .95
13.3.2 Accessing Online Help . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .95
13.3.3 Selecting the Language for Online Help. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .96
13.3.4 Using Online Help. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .96
13.3.5 Full-text Search with Logically Connected Search Terms . . . . . . . . . . . . . . . . .97
14 Changing the Specimen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .99
14.1 Changing the Specimen on an Upright Microscope . . . . . . . . . . . . . . . . . . . . . . . . .99
14.2 Changing the Specimen on an Inverted Microscope . . . . . . . . . . . . . . . . . . . . . . . .99
15 Changing the Objective . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .101
16 Piezo Focus on Upright Microscope . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .103
17 Switching Off the System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .105
17.1 TCS SP8/SP8 X with flexible supply unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .105
17.2 TCS SP8 with compact supply unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .110
18 Care and Cleaning. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .115
18.1 Cleaning Surfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .115
18.2 Cleaning the Optical System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .115
18.3 Cleaning Immersion Lenses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .116
18.4 Care . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .116
19 Repairs and Service Work. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .117
20 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .119
20.1 Having Coolant Replaced . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .119
21 Disassembly and Transport. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .121
22 Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .123

9
Contents
23 Contact . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .125
24 Appendix. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .127
24.1 Patents. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .127
24.2 Safety Data Sheets from Third-Party Manufacturers . . . . . . . . . . . . . . . . . . . . . . .127
24.3 Compliance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .133
24.4 People's Republic of China. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .134

10
Contents

11
About this User Manual
1 About this User Manual
Prior to commissioning the system, carefully read through this User Manual and be
absolutely certain to follow the safety notes contained in it. So that you can operate the
system safely and react quickly and correctly in the event of an emergency, you must
familiarize yourself with the safety devices before using it for the first time. In this case, read
Chapter "Safety Features" in this manual. Keep this User Manual and the included manuals
for the microscope and other components in a safe place easily accessible for all users.
This Manual gives you important information about safe handling of the system. All
information is intended for the safety of users and trouble-free operation of the system.
Unless the information pertains specifically to certain system variants, the instructions
always apply to the basic system described here.
This User Manual provides you with important information for using the system, the
necessary ambient conditions and the usable lasers. It explains system startup. The system
is assembled and disassembled by service technicians that have been authorized by Leica
Microsystems CMS GmbH. This is why unpacking, assembly and installation of the system
are not described in this manual. You can find an overview of the system and specifications
in the Chapter "System Overview and Properties". For information about special
configurations, such as optional lasers or specific objectives, refer to the respective
included manual.
This User Manual does not contain any information about basic optical principles or the
operating principle of microscopes, confocal systems and the like. If you are interested in
these topics or certain applications from the area of optics and confocal microscopy, you
can read more about them at the Leica Microsystems CMS GmbH knowledge portal: http://
www.leica-microsystems.com/science-lab/
The system is delivered with the latest version of the licensed "Leica Application Suite
Advanced Fluorescence" (LAS AF) software. In order to keep information up-to-date, a
description of software functions has been intentionally omitted from this operating manual.
You can find up-to-date explanations and instructions for corresponding software functions
in the LAS AF Online Help. Read Chapter "LAS AF" in this User Manual in order to familiarize
yourself with the design and basic operation of the software. Additional information about
specific functions can be found in the online help.
The instructions contained in this documentation reflect state-of-the-art technology and
knowledge standards at the time of publication. Leica Microsystems CMS GmbH reserves
the right to revise this documentation and/or to further develop and improve the products
described in this document at any time without prior notice or any other obligation.
If you have any suggestions or improvements for this User Manual, please contact the Leica
branch office in your country.

12
About this User Manual

13
Intended Use
2 Intended Use
This system is intended for use in a lab. The system was designed for confocal scanning
(laser scanning images) of fluorescence-marked living and fixed specimens as well as for
quantitative measurements in the area of life science.
Applications of in-vitro diagnostics in accordance with MPG (German Medical Devices Act)
are excluded from proper intended use.
This system must not be used together with life-support systems such as those found in
intensive-care wards.
The owner/operator and user of this product are responsible for proper and safe operation
and safe maintenance of the system and for following all applicable safety regulations. The
owner/operator and user are fully liable for all consequences resulting from the use of the
system for any purposes other than those listed in the User Manual or the online help.
The manufacturer assumes no liability for damage caused by, or any risks arising from, use
of the microscopes for purposes other than those for which they are intended, or not using
the microscopes within the specifications of Leica Microsystems CMS GmbH. In such
cases, the Declaration of Conformity shall be invalid.

14
Intended Use

15
Liability and Warranty
3 Liability and Warranty
Leica Microsystems CMS GmbH shall not be liable for damages resulting from failure to
observe the information in this User Manual. The information here does not in any way
modify the warranty and liability clauses contained in the general terms and conditions of
Leica Microsystems CMS GmbH.
Repairs and servicing may be performed only by service technicians authorized by Leica
Microsystems CMS GmbH. Opening or working on the system in any way shall void any and
all warranty claims.
The manufacturer assumes no liability for damage caused by, or any risks arising from, use
of the microscopes for purposes other than those for which they are intended, or not using
the microscopes within the specifications of Leica Microsystems CMS GmbH. In such
cases, the Declaration of Conformity shall be invalid.
Leica Microsystems CMS GmbH shall not be liable for any damage caused by incorrect
storage, improper transport or an unsuitable installation location.
Figures are for illustration purposes. The system you purchased can deviate from the
illustrations without Leica Microsystems CMS GmbH explicitly specifying such.
Leica Microsystems CMS GmbH shall not be liable for any injury or property damage caused
by untrained or unauthorized persons.
3.1 Important Information for Operators and Users
• The owner/operator is required to designate a Laser Safety Officer or a Laser Protection
Advisor according to the applicable legal requirements in each country.
• The owner/operator and user of this product are responsible for proper and safe
operation and safe maintenance of the system and for following all applicable safety
regulations.
• The owner/operator and user are fully liable for all consequences resulting from the use
of the system for any purposes other than those listed in the User Manual or the online
help.
• The owner/operator and user are obligated to perform and monitor suitable safety
measures (according to national regulations).
• The owner/operator and user are responsible for observing the laser safety regulations
according to applicable country-specific regulations.
• The owner/operator and user must ensure that this laser product is commissioned and
operated only by persons who have been trained in the use of the system and the
potential dangers of laser radiation.
• The owner/operator and user are fully liable for all consequences resulting from the use
of the system if it is opened, improperly serviced or repaired by persons other than
authorized Leica service representatives.

16
Liability and Warranty

17
Meaning of the warning messages in the manual
4 Meaning of the warning messages in the manual
WARNING Electric shock
This warns you of hazardous electrical voltage. Following the
instructions is mandatory, since otherwise there is a risk of severe or
fatal injury.
WARNING Severe injuries from ...
This note warns you of hazards that can cause severe or fatal injuries.
WARNING Permanent eye and skin damage from laser radiation
This note warns you of eye and skin damage that can occur when using
lasers if safety precautions are not taken.
WARNING Risk of injuries due to harmful or irritating substances
This note warns you of substances that pose a health hazard.
WARNING Risk of injuries due to biological substances
This note warns you of biological substances that pose a health hazard.
WARNING Risk of burns on hot surfaces
This note warns you of hot surfaces that can cause burns.

CAUTION Injuries from…
This note warns you of minor to moderate injuries that can be prevented
by following instructions.
NOTICE Risk of damage to the system
This note describes possible material damage that can occur in case of
misuse.
NOTICE Loss of data
D A T A
This note warns you of the potential for losing data.
Observe user manual
This mandatory sign indicates that an additional user manual must be
followed.
Wear laser safety glasses
This mandatory sign indicates that laser safety glasses must be worn to
prevent eye injuries.
Additional note
This note serves to emphasize important instructions for handling the
product or contains special instructions about a certain topic.
18
Meaning of the warning messages in the manual

19
General Safety Notes
5 General Safety Notes
You have to follow the instructions listed below to work with the instrument safely and
without disturbance. If you do not follow these or other instructions in this User Manual or
the included manual, Leica Microsystems CMS GmbH shall not be liable for any resulting
injury or property damage.
As it is impossible to anticipate every potential hazard, please be careful and apply common
sense when using the system.
5.1 Commissioning and Use
• The system components have been packaged securely for transport in multiple crates.
Do not open these crates. The crates may be opened and unpacked by Leica service
technicians or by people who are authorized by Leica Microsystems CMS GmbH only.
• The system may only be set up by Leica service technicians or by people who are
authorized by Leica Microsystems CMS GmbH.
• This laser equipment may be operated only by persons who have been trained in the use
of the system and about the potential hazards of laser radiation.
• Have your laser safety officer instruct you about the dangers of laser radiation and about
suitable laser safety precautions, such as wearing suitable laser safety glasses. This
applies to all persons present in the room where the system is set up and operated.
• Each user must have read the instructions included and follow the instructions it
contains.
• Do not introduce any flammable objects, such as paper, into the specimen area when
you are working with a laser.
• Do not place any flammable or combustible objects on or near the system and do not put
it near hot surfaces.
• During start up and during operation, you have to keep your hands and fingers away from
the specimen area, as otherwise there is a risk of crushing hazards or injury from rotating
objectives and the motorized specimen stage.
• Set up the workplace (for example, chair and monitor) on the system so that it
corresponds to your requirements. Observe the national regulations for occupational
safety.
• Before each service call by a service technician or whenever you relocate the
instrument, you have to clean it thoroughly. This is necessary to remove any possible
contamination, thereby preventing the transfer of dangerous substances and pathogens
and avoiding hazards and dangers. The same also applies to the removal of components.
This applies in particular to systems that are located in biomedical research labs.
• You must not deviate from the operating and maintenance instructions provided herein.
5.2 Modifications to the System
• The system is installed by service technicians from Leica Microsystems CMS GmbH. You
must not change the position of the system components.

20
General Safety Notes
• The supply unit must always be set up and operated in an upright position.
• Under no circumstances may you open housing parts.
• Never disconnect a fiber optic cable.
• The cable and fiber optic cable may not be folded, stretched, pinched or rolled up tightly
or damaged in any other way.
• The product has a closed liquid coolant circuit. The cooling liquid hoses may not be
folded, stretched, pinched or rolled up tightly or damaged in any other way.
• Do not connect any external equipment or other components. If you have questions,
please directly contact the Leica branch office in your country.
5.3 Safety Devices and Safety Labels
• So that you can operate the system safely and react quickly and correctly in the event of
an emergency, you must familiarize yourself with the safety devices before using it for
the first time. Read Chapter "Safety Features" of this User Manual carefully.
• Never remove the safety devices on the system.
• Never deactivate the laser protection devices.
• All safety devices must be ready to operate. Do not carry out any procedures that modify,
disable or damage the functionality of safety features. Unauthorized procedures could
result in serious injuries or property damage.
• Safety labels on the system may not be removed. Missing or damaged safety labels must
be attached immediately and at the described location. Observe Chapter "Safety Labels
on the System".
5.4 Laser Safety
• The instrument is a Class 3B/IIIb (VIS and UV lasers) or a Class 4/IV (MP systems) laser
product.
• You must observe all suitable safety measures applicable for this laser class.
• When using an MP system, you must wear laser safety glasses (Order No.: 158002570).
Appropriate laser safety glasses for IR laser radiation are provided with the system when
delivered. During the scanning operation, all persons present in the room must wear
such laser safety glasses. These laser safety glasses do not provide any protection
against visible laser radiation (visible spectrum)!
• It is not necessary to wear protective eyewear when using VIS and UV lasers. When
used as intended and safety notes have been followed, laser radiation is kept within the
limit value that eliminates the chance for eye injuries.
• Never look directly into a laser beam or a reflection of the laser beam. Avoid all contact
with the laser beam.
• Never expose your eyes or skin to direct or indirect laser radiation. The radiation can
cause irreparable eye and skin injuries.
• During the scanning operation, the laser radiation is accessible in the microscope's
specimen area without obstruction after coming out of the objective. Always maintain a