Leica HistoCore Arcadia C User manual

HistoCore Arcadia C
Cold Plate
Instructions for Use
English
Order No.: 14039380101 - Revision K
Always keep this manual with the instrument.
Read carefully before working with the instrument.
Version 1.8, Revision K - 10.2018

3
HistoCore Arcadia C
The information, numerical data, notes and value judgments contained in this Instructions for Use
represent the current state of scientific knowledge and state-of-the-art technology as we understand it
following thorough investigation in this field.
We are under no obligation to update the present Instructions for Use periodically and on an ongoing
basis according to the latest technical developments, nor to provide our customers with additional
copies, updates etc. of this Instructions for Use.
To the extent permitted in accordance with the national legal system as applicable in each individual
case, we shall not be held liable for erroneous statements, drawings, technical illustrations etc.
contained in this Instructions for Use. In particular, no liability whatsoever is accepted for any financial
loss or consequential damage caused by or related to compliance with statements or other information
in this Instructions for Use.
Statements, drawings, illustrations and other information regarding the contents or technical details of
the present Instructions for Use are not to be considered warranted characteristics of our products.
These are determined only by the contract provisions agreed between ourselves and our customers.
Leica Biosystems reserves the right to change technical specifications as well as manufacturing
processes without prior notice. Only in this way is it possible to continuously improve the technology
and manufacturing techniques used in our products.
This document is protected under copyright laws. All copyrights to this documentation are held by Leica
Biosystems Nussloch GmbH.
Any reproduction of text and illustrations (or of any parts thereof) by means of print, photocopy,
microfiche, web cam or other methods – including any electronic systems and media – requires express
prior permission in writing by Leica Biosystems Nussloch GmbH.
For the instrument serial number and year of manufacture, please refer to the nameplate on the back of
the instrument.
Note
Leica Biosystems Nussloch GmbH
Heidelberger Str. 17 - 19
D-69226 Nussloch
Germany
Tel.: +49 - (0) 6224 - 143 0
Fax: +49 - (0) 6224 - 143 268
Web: www.LeicaBiosystems.com
Assembly contracted to Leica Microsystems Ltd. Shanghai

4Version 1.8, Revision K
Table of contents
1. Important Information ................................................................................................................................ 5
1.1 Naming conventions ....................................................................................................................................... 5
1.2 Symbols in the text and their meanings....................................................................................................... 5
1.3 Instrument type................................................................................................................................................ 8
1.4 Intended use of instrument............................................................................................................................ 8
1.5 Qualification of personnel.............................................................................................................................. 8
2. Safety ........................................................................................................................................................... 9
2.1 Safety notes...................................................................................................................................................... 9
2.2 Warnings......................................................................................................................................................... 10
3. Instrument Components and Specifications........................................................................................ 12
3.1 Overview – instrument components........................................................................................................... 12
3.2 Main features of the instrument.................................................................................................................. 12
3.3 Technical Data............................................................................................................................................... 12
4. Setting up the instrument ........................................................................................................................ 14
4.1 Site requirement ............................................................................................................................................ 14
4.2 Standard delivery – packing list.................................................................................................................. 14
4.3 Unpacking and installation........................................................................................................................... 15
4.4 Moving the instrument.................................................................................................................................. 17
4.5 Power supply.................................................................................................................................................. 18
5. Operation.................................................................................................................................................... 19
5.1 Switching the instrument on ....................................................................................................................... 19
5.2 Replacing the secondary fuse..................................................................................................................... 19
6. Maintenance and Cleaning..................................................................................................................... 21
6.1 Cleaning the instrument................................................................................................................................ 21
6.2 Maintenance instructions............................................................................................................................ 21
7. Troubleshooting ........................................................................................................................................ 22
8. Warranty and Service .............................................................................................................................. 23
9. Decontamination Confirmation.............................................................................................................. 24

5
HistoCore Arcadia C
Important Information
1
1. Important Information
1.1 Naming conventions
Note
The full name of the device is HistoCore Arcadia C Cold Plate. The device is called
HistoCore Arcadia C to ensure that the Instructions for Use are well legible.
1.2 Symbols in the text and their meanings
Symbol: Title of the symbol: Warning
Description: Warnings appear in a white box, orange header and
are marked by a warning triangle.
Symbol: Title of the symbol: Note
Description: Notes, i. e. important user information, appear in
a white box, blue header and are marked by an
information symbol.
Symbol: Title of the symbol: Item number
→"Fig. 7-1"Description: Item numbers for numbering illustrations. Numbers in
red refer to item numbers in illustrations.
Symbol: Title of the symbol: Caution
Description: Caution, consult the instructions for use for
cautionary information.
Symbol: Title of the symbol: Power on
Symbol: Title of the symbol: Power off
Symbol: Title of the symbol: Consult Instructions for Use
Description: Indicates the need for the user to consult the
Instructions for Use.
Symbol: Title of the symbol: Manufacturer
Description: Indicates the manufacturer of the medical product.
Symbol: Title of the symbol: Manufacturing date
Description: Indicates the date when the medical device was
manufactured.

6Version 1.8, Revision K
Important Information
1
Symbol: Title of the symbol: Alternating current
Symbol: Title of the symbol: PE terminal
Symbol: Title of the symbol: Article number
Description: Order number for standard delivery or accessories.
Symbol: Title of the symbol: Serial number
Description: Designates the serial number of the instrument.
Symbol: Title of the symbol: China RoHS
Description: Environmental protection symbol of the China RoHS
directive. The number in the symbol indicates the
"Environment-friendly Use Period" of the product in
years. The symbol is used if a substance restricted
in China is used in excess of the maximum permitted
limit.
Symbol: Title of the symbol: WEEE Symbol
Description: Symbol for labeling electrical and electronic
equipment in accordance with Section 7 of the
German Electrical and Electronic Equipment Act
(ElektroG). ElektroG is the law regarding the sale,
return and environmentally sound disposal of
electrical and electronic equipment.
Symbol: Title of the symbol: CE Compliance
Description: CE labeling shows that the product corresponds to
one or more applicable European directives.
Symbol: Title of the symbol:
This product fulfills the requirements of the CAN/
CSA-C22.2 No. 61010.
Symbol: Title of the symbol: Fragile, handle with care
Description: The package contents are fragile and must be
handled with care.
Symbol: Title of the symbol: Keep dry
Description: The package must be kept in a dry environment.

7
HistoCore Arcadia C
Important Information
1
Symbol: Title of the symbol:
Indicates the correct upright position of the package.
Symbol: Title of the symbol:
It allows maximum 2 stacks layers.
Symbol: Title of the symbol:
Indicates the temperature range permitted for storing
and transporting the package.
Minimum -29 °C
Maximum +50 °C
Symbol: Title of the symbol:
Indicates the temperature range permitted for storing
the package.
Minimum +5 °C
Maximum +50 °C
Symbol: Title of the symbol:
Indicates the humidity range permitted for storing
and transporting the package.
Minimum 10 % r.H.
Maximum 85 % r.H
Symbol: Title of the symbol:
Tip-n-Tell indicator to monitor whether the shipment
has been transported and stored in upright position
according to your requirements. With a pitch of
60° or more, the blue quartz sand flows into the
arrow-shaped indicator window and sticks there
permanently. Improper handling of the shipment
is immediately detectable and can be proven
definitively.
Symbol: Title of the symbol:
In the Shockwatch system, a shock dot shows
shocks or impacts that are above a specified
intensity through red coloration. Exceeding a defined
acceleration (g value) causes the indicator tube to
change color.

8Version 1.8, Revision K
Important Information
1
Symbol: Title of the symbol:
Indicates the item can be recycled where correct
facilities exist.
Symbol: Title of the symbol: Regulatory Compliance Mark (RCM)
Description The Regulatory Compliance Mark (RCM) indicates a
device’s compliance with applicable ACMA technical
standards of New Zealand and Australia - that is, for
telecommunications, radio communications, EMC
and EME.
1.3 Instrument type
All information provided in these Instructions for Use applies only to the instrument type indicated on
the cover page.
A nameplate is attached to the back of the instrument and a serial number label is on the side of the
instrument.
1.4 Intended use of instrument
The HistoCore Arcadia C is a cold plate for chilling and blocking out histological tissue samples in
paraffin blocks.
Any other use of the instrument will be considered as improper use!
1.5 Qualification of personnel
• The HistoCore Arcadia C may be operated by trained laboratory personnel only.
• All laboratory personnel designated to operate this instrument must read these Instructions for Use
carefully and must be familiar with all technical features of the instrument before attempting to
operate it.

9
HistoCore Arcadia C
Safety
2
2. Safety
2.1 Safety notes
Warning
The safety and caution notes in this chapter must be observed at all times. Be sure to read these
notes even if you are already familiar with the operation and use of other Leica Biosystems products.
These Instructions for Use include important instructions and information related to the operating safety
and maintenance of the instrument.
These Instructions for Use are an important part of the product, and must be read carefully prior to
startup and use and must always be kept near the instrument.
This instrument has been built and tested in accordance with the safety requirements for electrical
equipment for measurement, control, and laboratory use.
To maintain this condition and ensure safe operation, the user must observe all notes and warnings
contained in these Instructions for Use.
Note
These Instructions for Use must be appropriately supplemented as required by the existing
regulations on accident prevention and environmental safety in the operator's country.
Warning
The protective devices on the instrument and its accessories must not be removed or modified.
Only service personnel qualified by Leica Biosystems may repair the instrument and access the
instrument's internal components.
Warning
Use only the provided power cable - this must not be replaced with a different power cable. If the
power plug does not fit in your socket, contact our service.
Warning
Residual risks
The instrument has been designed and constructed with the latest state-of-the-art technology and
according to recognized standards and regulations with regard to safety technology. Operating or
handling the instrument incorrectly can place the user or other personnel at risk of injury or can
cause damage to the instrument or other property. The instrument may be used only as intended and
only if all of its safety features are in proper working condition. Malfunctions that impede safety must
be remedied immediately.

10 Version 1.8, Revision K
Safety
2
Note
For current information about applicable guidelines, please refer to the CE declaration of conformity
and on our Internet site at:
http://www.LeicaBiosystems.com
Warning
To prevent damage to the instrument or the specimen, only accessories authorized by
Leica Biosystems may be used.
2.2 Warnings
The safety devices installed in this instrument by the manufacturer only constitute the basis for accident
prevention. Operating the instrument safely is, above all, the responsibility of the owner, as well as the
designated personnel who operate, service or repair the instrument.
To ensure trouble-free operation of the instrument, make sure to comply with the following instructions
and warnings.
Warnings – Safety notes on the instrument itself
Warning
• This device may only be used by trained laboratory technicians. It must only be operated for the
purpose of its designated use and according to the instructions contained in these Instructions for
Use.
• Safety notes on the instrument itself, which are marked with a warning triangle, indicate that the
correct operating instructions (as defined in these Instructions for Use) must be followed when
operating or replacing the item marked. Nonobservance can cause accidents, injuries and/or
damage to the instrument/accessories.
Safety instructions - Transport and installation
Warning
• After unpacking the instrument it may only be transported in an upright position.
• Before connecting the device to a power source, ensure that the voltage indicated on the type
plate matches the voltage available at the place of installation.
• The unit must be connected only with the supplied power cable and only to a grounded power
receptacle. Do not use an extension cord.
• The power socket to which the instrument is connected has to be near the instrument and easily
accessible.

11
HistoCore Arcadia C
Safety
2
Warning
• The minimum voltage must be maintained while starting the refrigeration unit (→p. 12 – 3.3
Technical Data).
• The compressor needs a start-up current of approx. 25 A. A stable power supply in accordance
with the instrument's specifications is essential to its proper functioning. Please ensure that your
electrical installation fulfills these preconditions prior to installing the unit. Nonobservance causes
damage to the instrument.
• Switch off the instrument each time before servicing, repairing or cleaning, and pull out the power
plug.
• Failure to observe the instructions specified by the manufacturer may result in damage to the
protection provided by the instrument.

12 Version 1.8, Revision K
Instrument Components and Specifications
3
3. Instrument Components and Specifications
3.1 Overview – instrument components
Instrument front view
4
1
2
3
5
6
Fig. 1
1Power switch 4Heat sink
2Refrigeration system (inside) 5Port for power inlet
3Cooling surface 6AC fuses
3.2 Main features of the instrument
• The instrument is distinguished by a simple, modular design and a powerful refrigeration unit with
precisely controlled cooling performance.
• The environment adaptive control module ensures the working temperature always stabilized at
-6 °C.
• High cooling performance ensures that the instrument's working temperature is reached quickly.
• Optimized temperature distribution in the cold plate prevents dripping condensation.
• The generously-dimensioned cooling surface has room for around 60 blocks.
• Designed to be used with the HistoCore Arcadia H Paraffin Embedding Station.
3.3 Technical Data
General data
Voltage of power supply 100 VAC, 110-120 VAC, 220-240 VAC,
50/60Hz
Fuse Time-lag fuses 5 x 20 mm
220-240 VAC: 2xT5A, 250V
100-120 VAC: 2xT10A, 250V
Nominal current 5 A max.
Maximum start-up current (5 s) 25 A

13
HistoCore Arcadia C
Instrument Components and Specifications
3
Environmental operating temperature range +20 °C to +30 °C
Operating temperatures -6 °C
Environmental relative humidity 20 to 80 % - non-condensing
Environmental operating altitude Up to 2000 m
Permissible temperature range during storage +5 °C to +50 °C
Permissible temperature range during transport -29 °C to +50 °C
Permissible humidity range during storage and transport 10 to 85 % - non-condensing
Electromagnetic environment Basic electromagnetic environment
IEC 61010 classification Protection class 1
Pollution degree 2
IP protection class (IEC 60529) IP20
Refrigeration unit
Refrigeration capacity* 158 W( at 50Hz) ;185 W( at 60Hz)
Safety factor 3
Refrigerant R 134a
Compressor oil 150 +10/-5 ml Ester RL7H, ISO 7
* according to ASHRAE, condensing temperature: 54.4 °C, evaporating temperature: -23.3 °C
Coolant 115 g ± 2 g
Dimensions and weights
for instrument Order No. 14 0393 53599, 14 0393 54102, 14 0393 54092,14 0393 54091
Width: 400 mm
Depth: 605 mm
Height: 385 mm
Weight: 30 kg
Dimensions and weights
for instrument Order No. 14 0393 57262, 14 0393 57261, 14 0393 57260, 14 0393 57263
Width: 400 mm
Depth: 636 mm
Height: 384 mm
Weight: 32 kg

14 Version 1.8, Revision K
Setting up the instrument
4
4. Setting up the instrument
4.1 Site requirement
• Stable, vibration-free laboratory table with horizontal, flat table top, as far as possible vibration-free
ground.
• No direct sunlight or strong temperature fluctuations. Room temperature consistently between +20 °C
and +30 °C.
• Relative air humidity maximum 80 %, non-condensing.
• The instrument should be set up in such a way that the air circulation is not impaired.
• The instrument must be installed in a place that ensures an easy disconnection from the power
supply. The power cable must be in a place that can be easily reached.
Warning
At a room temperature of > +30 °C, the working temperature of the cold plate of -6 °C may not be
reached at all points.
Warning
To ensure proper function and an easy disconnection of the power cable from the instrument, there
must be gap of at least 15 cm behind the instrument. Failure to observe this distance may result in
serious damage to the refrigeration unit of the device. The instrument should not be operated in
hazardous locations.
4.2 Standard delivery – packing list
Qty Designation Order No.
1 Basic unit HistoCore Arcadia C
220-240 VAC 14 0393 53599
220-240 VAC, China 14 0393 54102
110-120 VAC 14 0393 54092
100 VAC 14 0393 54091
220-240 VAC 14 0393 57262
220-240 VAC, China 14 0393 57263
110-120 VAC 14 0393 57261
100 VAC 14 0393 57260
4 Sets of spare fuses: 220-240 VAC, 5A 250 V 14 6000 05015
100-120 VAC, 10A 250V 14 6000 05078
1 Instructions for Use (printed English with language CD 14 0393 80200) 14 0393 80001
The country specific power cord needs to be ordered separately. Please find a list of all power cords
available for your device on our website www.LeicaBiosystems.com within the product section.

15
HistoCore Arcadia C
Setting up the instrument
4
Note
Please compare the delivered components against the packing list and your order. Should there be
any discrepancy, please contact the Leica Biosystems distributor handling your order.
4.3 Unpacking and installation
Note
When the instrument is delivered, check the tilt indicators on the packaging. If the
arrowhead is blue, the shipment was transported laying flat, was tilted at too great an
angle or fell over during transport.
Note this on the shipping documents and check the shipment for possible damage.
Warning
These unpacking instructions only apply if the box is placed with the symbols facing upwards.
1. Remove the packing strap (→Fig. 2-1)and the adhesive tape (→Fig. 2-2).
2. Open the package. Lift up and remove the carton wall (→Fig. 2-3).
1
2
3
Fig. 2

16 Version 1.8, Revision K
Setting up the instrument
4
3. Remove the foam pads (→Fig. 3-1)one by one.
1 1
Fig. 3
Warning
The HistoCore Arcadia C always has to be transported upright and horizontally. It must not be
inverted under any circumstances, even for short periods, or stored on one of its sides.
It is mandatory to observe a waiting time of 4 hours between the last transport and the first time
the instrument is switched on. The oil present in the compressor needs this time to flow back to its
original location.
4. Ensure that when removing the instrument (→Fig. 4-1)from the pallet this is carried out by
two people lifting four lower corners of the housing base (→Fig. 4).
5. Place the instrument on a stable laboratory table.
1
Fig. 4
6. Remove the accessories from the accessory box (→Fig. 5-1)on the base of the pallet.

17
HistoCore Arcadia C
Setting up the instrument
4
1
Fig. 5
Note
The packaging must be retained for the duration of the warranty period. To return the instrument,
follow the instructions above in reverse order.
4.4 Moving the instrument
Warning
Do not move the instrument during operation.
Before moving the instrument, make sure that there is no specimen blocks on the cold plate, the
instrument is at an ambient temperature, and the power cord is disconnected from the power supply.
Do not touch the metal parts of the compressor air outlet (→Fig. 6-1)on the rear panel. It is
mandatory to observe a waiting time of 4 hours before the instrument is switched on.
Hold the instrument at the front and rear part of the lower housing base and move.
1
Fig. 6

18 Version 1.8, Revision K
Setting up the instrument
4
4.5 Power supply
The HistoCore Arcadia C refrigeration unit requires a specific voltage and frequency (→p. 12 – 3.3
Technical Data), and is therefore always delivered with a power cord that fits the instrument.
Please observe the following notes to prevent damage to the instrument.
Warning
Before connecting the instrument to the power supply, it is mandatory to check whether the voltage
specified on the identification label (rear side) matches the actual voltage values at the installation
location.
If this is not the case, the connection must not be made!
The unit must be connected only with the supplied power cord and only to a grounded power
receptacle.
Do not use an extension cord!
1. Connect the power cord plug (→Fig. 7-2)to the connecting port (→Fig. 7-1).
1
2
Fig. 7
2. Plug the power cord into the wall outlet.

19
HistoCore Arcadia C
Operation
5
5. Operation
5.1 Switching the instrument on
After installation as described in (→p. 14 – 4. Setting up the instrument), the HistoCore Arcadia C
is ready for operation. Switch on the device with the Power switch at the front left of the instrument
("I" = ON). A lamp lights up in the switch to indicate that the unit is operational and the refrigeration unit
will start working.
Depending on the room temperature, the time to reach the target temperature of the cooling surface
(-6 °C) will be around 25 minutes.
Fig. 8
Warning
The cooling surface may not be loaded with molds until the cooling time has elapsed. Otherwise, the
working temperature of -6 °C may not be reached.
Note
The compressor will start to work in five minutes after the power is on.
5.2 Replacing the secondary fuse
A miniature fuse to protect the electronic components is located on the rear of the instrument.
Fuse rating: 220-240 VAC, 5A 250 V
100-120 VAC, 10A 250 V
Warning
Before replacing the fuse, always switch the instrument off and pull the power plug from the wall
socket.
Only miniature fuses of the type specified can be used (→p. 12 – 3.3 Technical Data).

20 Version 1.8, Revision K
Operation
5
To replace the fuse, please proceed as follows:
1. Use a screwdriver to open the fuse holder (→Fig. 9-1)and remove the fuses (→Fig. 9-2).
2
1
Fig. 9
2. Replace them with two new fuses of the same type.
3. Use the screwdriver to press the fuse holder back to its original location.
4. Reconnect the instrument to an AC power outlet and switch it on.