Leica MICA User manual

1
Leica Microsystems CMS GmbH. User Manual, 11xxxxxx, revision 1.0, 2021-12-15
MICA
User Manual
From Eye to Insight

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Copyrights
Revision 1.0, edited 2020-12-15 by:
Leica Microsystems CMS GmbH
Ernst-Leitz-Strasse 17–37
D-35578 Wetzlar (Germany)
http://www.leica-microsystems.com
Responsible for contents:
Marketing CMS
Copyrights
All rights to this documentation are held by Lei-
ca Microsystems CMS GmbH. Reproduction of
text or illustrations (in whole or in part) by print,
photocopy, microfilm or other method (including
electronic systems) is not allowed without ex-
press written permission from Leica Microsys-
tems CMS GmbH.
The instructions contained in the following doc-
umentation reflect state-of-the-art technology.
We have compiled the texts and illustrations
as accurately as possible. Still, we are always
grateful for comments and suggestions regarding
potential errors within this documentation.
The information included in this manual may be
changed without prior notice.

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Contents
Contents
1. Important Notes about this Manual.............4
2. Function............................................................6
3. Safety Notes.....................................................9
3.1 Important Information for Operators and
Users .................................................................9
3.2 General Safety Notes .....................................9
3.3 Electrical Safety ............................................10
3.4 Specifications of the Lasers
Installed in the Supply Unit..........................13
3.5 Laser Safety ...................................................13
3.5.1 Safety notes for handling the laser ............13
4. Transport and Storage..................................15
4.1 Transport ........................................................15
4.2 Ambient Conditions.......................................15
4.3 Installation Location .....................................16
4.4 Disposal ..........................................................17
5. Overview of the Instrument.........................18
5.1 Mica 18
5.1.1 Status LED ......................................................18
5.2 Supply Unit .....................................................20
5.3 Incubator ........................................................21
5.4 Auto Immersion ............................................21
5.5 CORR objectives ...........................................21
5.6 Antivibration Table........................................22
6. Instrument Setup...........................................23
6.1 Switching on ..................................................23
6.2 Notes on Handling the Instrument Hood...23
7. Operation........................................................24
7.1 Starting the software....................................24
7.2 Software help.................................................24
7.3 Online Help .....................................................24
7.4 The User Interface ........................................25
7.5 First Experiment.............................................33
8. Care ...............................................................34
9. EU-Declaration of Conformity.....................35

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1. Important Notes about this Manual
(1.2)
→p. 20
Numbers in parentheses refer to illustrations; for
example, (1.2) refers to Fig. 1, item 2.
Numbers with a pointer arrow (for example
→page 20), point to a certain page of this man-
ual.
WARNING indicates a hazard with a medium
degree of risk that, if not avoided, can result
in death or serious injury.
CAUTION indicates a potentially hazardous
situation with a low degree of risk that, if not
avoided, may result in minor or moderate in-
jury.
Caution!
In this manual, additional safety notes are in-
dicated with the triangle symbol shown here,
and have a gray background.
Caution! The instrument and its accessories can
be damaged when operated incorrectly.
Text Symbols and Their Meanings:
Caution!
This User Manual is an essential component
of the product; it must be read carefully be-
fore the product is assembled, put into op-
eration or used, and must be kept for future
reference.
1. Important Notes about this Manual
This User Manual includes important instruc-
tions and information related to the operating
safety and maintenance of the instrument. .
!
WARNING
CAUTION

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1. Important Notes about this Manual
Warning of hazardous electrical voltage! Danger
of electric shock!
Warning of optical radiation! Never look directly
into the light beam! Wear safety goggles!
Warning of electromagnetic field
Warning of UV radiation! Wear safety goggles!
Warning of hazardous laser radiation – take ap-
propriate safety precautions!
Warning of hand injuries
Instructions for disposing of the instrument,
its accessories and consumables.
Ground terminal
Explanatory note
Item not contained in all equipment configura-
tions
Manufacturing date, e.g. 11 / 2011 for November
2011
China RoHS 50 years EFUP
(Environmentally friendly use period)
*
MM/YYYY

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2. Function
2. Function
The entire system of which this User Manual is
part is intended for research applications. The
Leica microscope Mica is an inverted light mi-
croscope and intended for use as a general lab
microscope for routine examinations of biologi-
cal specimens.
Directives of the European Community (EU Di-
rectives)
The microscope described here fulfills the re-
quirements of EU Directives 2014/35/EU concern-
ing the safety of electrical equipment, 2014/30/EU
concerning electromagnetic compatibility and
the Machinery Directive 2006/42/EC.
According to the VO1194/2012 regulation regard-
ing the implementation of the Ecodesign Direc-
tive 2009/125/EC, microscopes are considered
special products. The light sources in this instru-
ment are special lamps which are only suitable
for microscopes. They are not suitable for gener-
al lighting purposes or room lighting.
Reasonably Foreseeable Misuse
The following are prohibited:
• To use the system for any purpose
not in accordance with the Declara-
tion of Conformity (e.g. the use of IVD or
medical products in accordance with
EU regulation 2017/746 or 2017/45 )
• Operating the system in an inclined position
• Cleaning the system in a way other than speci-
fied in the manual
• Modifying the built-in safety circuits in the in-
strument
• Allowing unauthorized personnel to open the
instrument
• Using cables that Leica Microsystems CMS
GmbH has not provided or permitted
• Using combinations with non-Leica compo-
nents that go beyond the scope of the manual

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2. Function
Caution!
The manufacturer assumes no liability for
damage caused by, or any risks arising from,
using the microscope for other purposes than
those for which they are intended or not us-
ing them within the specifications of Leica
Microsystems CMS GmbH.
In such cases, the Declaration of Conformity
shall be invalid.
WARNING
Notes on Handling Laser Devices
In the standard design without additional la-
ser safety precautions, the microscopes are
not suitable for coupling laser radiation (such
as to the camera ports), since this radiation
poses a hazard for the user (eye injury in par-
ticular).
For use of the microscope with lasers, Leica
Microsystems CMS GmbH offers special mi-
croscope variants with additional safety fea-
tures. Laser couplings require corresponding
safety devices that have to be inspected and
installed by trained personnel.
For further information, please contact your
authorized Leica Microsystems CMS GmbH
representative.
WARNING
Due to the special sources of danger of the
laser system, only those persons may use the
system who have received special training in
working with the system, observing the spe-
cific hazards of a laser system.
CAUTION
Light sources pose a potential irradiation risk
(glare, UV radiation, IR radiation). Therefore,
lamps may only be operated in closed hous-
ing and in an installed condition.
Never look directly into the beam path
(blinding hazard).

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2. Function
CAUTION
If the hood to the specimen area is open
and the UV LED (365nm) is switched on with
maximum configured intensity, a distance
between the eye and light outlet interface on
the objective of <10cm may result in a hazard
to the eye.
No hazard for the eye exists in the normal use
case (distance between eye and objective
>10cm).
When changing specimens with the UV LED
(365nm) switched on at maximum intensity,
be sure not to exceed a maximum exposure
time of the skin (hands) of 60 minutes a day.
(actinic UV hazard)
We recommend dimming/deactivating all
LEDs before opening.

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3. Safety Notes
3. Safety Notes
3.1 Important Information for Operators and
Users
• The owner/operator and user of this product
are responsible for proper and safe operation
and safe maintenance of the system and for
following all applicable safety regulations.
• The owner/operator and user are fully liable
for all consequences resulting from the use of
the system for any purposes other than those
listed in the User Manual or the online help.
• The owner/operator and user are obligated to
perform and monitor suitable safety measures
(according to national regulations).
• The owner/operator and user are responsible
for observing the laser safety regulations ac-
cording to applicable country-specific regula-
tions.
• The owner/operator and user must ensure that
this laser product is started up and operated
only by persons who have been trained in the
use of the system and the potential dangers of
laser radiation.
• The owner/operator and user are fully liable
for all consequences resulting from the use of
the system if it is opened, improperly serviced
or repaired by persons other than authorized
Leica service representatives.
3.2 General Safety Notes
This protection class 1 instrument was built and
tested in accordance with
EN 61010-1/IEC 61010-1, Safety requirements for
electrical equipment for measurement, control
and laboratory use.
It also meets the requirements of EN / IEC 61326-
1, Electrical equipment for measurement, control
and laboratory use – EMC requirements.
The product has also been tested in accordance
with EN 60825-1 / IEC 60825-1 Safety of laser
products
Part 1: Equipment classifications and require-
ments checked.
The products were also tested in accordance
with IEC 62471 "Photobiological safety of lamps
and lamp systems" and are a risk of risk group
2 (medium risk). Observe the warning message
regarding optical radiation on the previous page.
Caution!
In order to maintain this condition and to en-
sure safe operation, the user must follow the
instructions and warnings contained in this
User Manual.
Also note the provided User Manuals of other
components (e.g. incubator, Mot.Corr, etc.).

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3. Safety Notes
The instruments and accessories described
in this manual have been safety-tested and
checked for possible hazards.
The responsible Leica Microsystems CMS
GmbH affiliate or the main plant in Wetzlar
must be consulted whenever the instrument
is altered, modified or used in conjunction
with non-Leica components that go beyond
the scope of this manual.
Unauthorized alterations to the device or
noncompliant use shall void all rights to any
warranty claims and product liability!
In addition to this manual, the safety notes
of the other manuals provided must be ob-
served!
The system may be set up, installed and put
into operation only by authorized Leica per-
sonnel!
Caution!
The microscope may be operated by autho-
rized personnel only.
3.3 Electrical Safety
General Specifications
In addition, observe the information about the
specifications in the other User Manuals provid-
ed!
Supply Unit
For indoor use only.
Supply voltage: 100 to 240 VAC
Frequency: 50 / 60 Hz
Power consumption: max. 220 VA
Fuses: 4 A, slow-blowing,
Breaking capacity H, 250 VAC,
Size: 5x20 mm
Ambient temperature: 15 – 35 °C
Relative humidity: 90 % up to 30 °C,
non-condensing
Protection class: I
Overvoltage category: II
Pollution degree: 2

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3. Safety Notes
WARNING
The power plug may only be plugged into an
outlet equipped with a grounding contact.
Do not invalidate the grounding function by
using an extension cord without a ground
wire. Any interruption of the ground wire in-
side or outside of the instrument, or release
of the protective conductor terminal, can
cause the instrument to become hazardous.
Intentional ground interruption is not permit-
ted!
Caution!
By connecting it to the grounding connection
(ground screw at the back of the electron-
ics box), ancillary equipment connected to
the microscope may be brought to the same
ground wire potential with its own and/or
extra power supply. For connections with-
out a ground protection conductor, Leica
Microsystems CMS GmbH Service must be
consulted.
Caution!
Never use any fuses as replacements other
than those of the types and the current rat-
ings listed here. Using other fuses or bridging
the fuse holder is not permitted. The use of
other fuses poses a fire hazard.
WARNING
Before replacing the fuses, be absolutely
certain to switch off the main power switch
and remove the power cable.
Caution!
The microscope's electrical accessory compo-
nents are not protected against water. Water
can cause electric shock.
CAUTION
Unplug the power plug before performing
cleaning and maintenance work!
Protect electrical components from mois-
ture!
Caution!
Do not plug in or unplug data lines and con-
trol circuits unless the instrument has been
shut down; otherwise, the instrument may be
damaged.
WARNING
By definition, the main circuit breaker of this
instrument is the connection between the
power cable and instrument port. The user
must ensure unobstructed access to the
main circuit breaker at all times.

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3. Safety Notes
WARNING
Only use power cables, or alternative cables
with VDE/HAR codes that minimally fulfill the
following conditions: 3x0.75 mm2 and 10 A /
250 V.
WARNING
Electrical components must be assembled
at least 10cm from the wall and away from
flammable substances.
WARNING
In spite of the low speed, there is a risk of
pinching and crushing injuries when moving
the scanning stage.
Never put objects or body parts in the travel
path!
CAUTION
Light sources pose a potential irradiation risk
(glare, UV radiation, IR radiation). Therefore,
lamps may only be operated in closed hous-
ings and in an installed condition.
Never look directly into the beam path
(blinding hazard).
If the hood to the specimen area is open and
the UV LED (365nm) is switched on with con-
figured intensity, a distance between the eye
and light outlet interface on the objective of
<10cm may result in a hazard to the eye.
No hazard for the eye exists in the normal use
case (distance between eye and objective
>10cm).
When changing specimens with the UV LED
(365nm) switched on at maximum intensity,
be sure not to exceed a maximum exposure
time of the skin (hands) of 60 minutes a day.
(actinic UV hazard)
We recommend dimming/deactivating all
LEDs before opening.

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3. Safety Notes
3.4 Specifications of the Lasers Installed in the
Supply Unit
LASER CLASS 3B
Wavelengths of the lasers contained in the laser
box:
405 nm, 488 nm, 561 nm, 638 nm
Typical outputs:
405 nm →300 mW
488 nm →60 mW
561 nm →20 mW
638 nm →120 mW
Warm-up time: approx. 5 min
Laser type: CW laser (continuous wave)
The specified power outputs are the maximum
laser outputs of the individual laser diodes. The
actual laser power in the specimen plane is just
a fraction of this.
The lasers used correspond to laser class 3B.
The overall MICA system meets the requirements
of Laser Class 1.
3.5 Laser Safety
The supply unit is part of the system and operat-
ed in the context of one of the possible combi-
nations. The laser fiber is coupled to the system.
The system in all displayed combination options
is laser class 1 system in accordance with the re-
quirements of the IEC 60825-1.
The supply unit includes lasers of Class 3B. Due
to the laser safety measures, only radiation of la-
ser class 1 is found in the specimen area of the
microscope.
As long as all laser protection devices are cor-
rectly installed, you do not have to take any spe-
cial safety precautions.
The laser may be operated only when the com-
plete system is fully installed. The laser beam is
completely enclosed until it exits the objective.
The laser function can only be used with the
hood/lid of the instrument closed.
3.5.1 Safety notes for handling the laser
WARNING
The user is not permitted to open the instru-
ment!
The laser system is encapsulated. It may be
repaired or replaced by personnel authorized
by Leica CMS only. Incorrect handling of the
laser can cause injuries!

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3. Safety Notes
WARNING
Never deactivate the laser protection devic-
es.
WARNING
Never look directly into a laser beam or a re-
flection of the laser beam. Avoid all contact
with the laser beam.
WARNING
Do not introduce any reflective objects into
the laser beam path.
WARNING
All unoccupied positions in the objective
nosepiece must be closed using the supplied
covers.
WARNING
Never disconnect a fiber optic cable.
Caution!
Only persons authorized by Leica CMS are per-
mitted to open the supply unit.
WARNING
If other operation or adjustment devices than
those listed here are used or other proce-
dures are carried out, dangerous beam expo-
sure may result.
Risk of injury!
WARNING
To be classified as a Class 1 laser product ac-
cording to IEC/EN60825-1 and fulfill the elec-
trical safety regulations, all safety devices,
safety interlocks and safety systems of the
laser product must be in operational condi-
tion.
Deactivating or damaging these safety de-
vices or any intervention in any of these
safety devices may lead to serious eye inju-
ries, physical injuries or property damage. In
these cases, Leica Microsystems CMS shall
not assume any liability.

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4. Transport and Storage
4.1 Transport
Caution!
Transport and storage at -20 °C to +85 °C and
max. 90% humidity.
For shipping or transporting the system and its
accessory components, the original packaging
should be used.
WeightCAUTION
At a full load, the system weighs more than
230 kg. The user must take corresponding
precautions for transporting the instrument.
The microscope with the table may only be
moved/shifted by rolling it on the castors at
the table. Lifting the table with the micro-
scope is prohibited.
Stow the supply unit below the microscope
table. Castors are attached at the bottom of
the table for this purpose as well. The supply
unit may only be lifted by a team of 2 persons.
In this case, hold the supply unit at its bottom.
• Mica including antivibration table 190 kg
• Supply unit 40 kg
Caution!
The supply unit and the desk may only be
rolled, not lifted.
4.2 Ambient Conditions
Caution!
This system must not be used at elevations of
more than 2000 m above sea level.
This system, in combination with the incuba-
tor, must not be exposed to direct sunlight or
be located below a air-conditioning system,
as this could have an impact on the tempera-
ture control and/or condensation.
Caution!
Protect the system from excessive tempera-
ture fluctuations. Such fluctuations can lead
to the accumulation of condensation, which
can damage the electrical and optical com-
ponents.
Operating temperature: 15 – 35°C.
4. Transport and Storage

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4. Transport and Storage
Caution!
Do not use this instrument near sources of
high electromagnetic radiation (for example,
unshielded, intentionally operated ultra-high
frequency sources), because these can dis-
rupt proper operation.
We recommend operating the instrument in
ESD-protected areas as well assessing the elec-
tromagnetic environment and giving correspond-
ing instructions before operating this instrument.
This instrument has been developed and test-
ed in accordance with CISPR 11 Class A. It can
cause radio interference in a household environ-
ment. In this case, you may have to take mea-
sures to eliminate the interference.
We recommend assessing the electromagnet-
ic environment before setting up the instrument
and then giving corresponding instructions.
4.3 Installation Location
Work with the system should be performed in
a dust-free room that is free of oil vapors, other
chemical vapors and extreme humidity. At the
workstation, large temperature fluctuations, di-
rect sunlight and vibrations should be avoided.
These conditions can distort measurements and
longer microscopic scans.
Permitted ambient conditions:
Temperature 15 – 35 °C
Relative humidity maximum 90% up to 30°C
Microscopes in warm and warm-damp climatic
zones require special care in order to prevent
fungus contamination. Also make sure to follow
the installation instructions in the microscope's
manual!
Caution!
The supply unit must be operated in upright
position because otherwise the function of
the safety device is not guaranteed.
Caution!
The supply unit must be set up so that the
power voltage input is not obstructed and the
instrument can quickly be disconnected from
the power supply if necessary.

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4. Transport and Storage
Caution!
Electrical components must be assembled
at least 10 cm from the wall and away from
flammable substances.
Caution!
Do not cover the ventilation slots. Risk of
overheating
4.4 Disposal
Once the product has reached the end of its
service life, please contact Leica Microsystems
GmbH Service or Sales about disposal.
Please observe and comply with the national and
federal laws and regulations implementing the
WEEE EC directive, for example.
Note!
Like all electronic devices, the microscope,
its accessory components and consumables
must never be disposed of with general
household waste.

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5. Overview of the Instrument
5.1 Mica
5. Overview of the Instrument
5.1.1 Status LED
Status of LED Status
Off Instrument switched off
White All components are initialized
White 2 sec. flashing Experiment in progress
Red 2 sec. flashing Incubator heating up. The desired temperature has not been
reached yet
White 0.1 sec. flashing Ongoing experiment has been canceled
Abb. 1 Overview of the Instrument Mica
1 Hood
2 Status indicator
3 SmartMove
1
2
3

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5. Overview of the Instrument
• Mica without antivibration table 100 kg
• MICA including antivibration table 190 kg
• Supply unit 40 kg
657

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5. Overview of the Instrument
5.2 Supply Unit
Before you can switch on the instrument using
the Power on/off push button on the side, make
sure that the I/O main switch on the rear side of
the supply unit is in position I and the power ca-
ble is inserted.
1. Power on/off: Press switch once to switch on.
Press and hold for 3 seconds to switch off.
2. Interlock key: When the lasers are not needed,
the key can be switched off.
3. Interlock indicator: Lights up when the inter-
lock is switched on and the lasers are avail-
able.
1
2
3