Leica MICA User manual

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Leica Microsystems CMS GmbH. User Manual, 11xxxxxx, revision 1.0, 2021-12-15
MICA
User Manual
From Eye to Insight

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Copyrights
Revision 1.0, edited 2020-12-15 by:
Leica Microsystems CMS GmbH
Ernst-Leitz-Strasse 17–37
D-35578 Wetzlar (Germany)
http://www.leica-microsystems.com
Responsible for contents:
Marketing CMS
Copyrights
All rights to this documentation are held by Lei-
ca Microsystems CMS GmbH. Reproduction of
text or illustrations (in whole or in part) by print,
photocopy, microfilm or other method (including
electronic systems) is not allowed without ex-
press written permission from Leica Microsys-
tems CMS GmbH.
The instructions contained in the following doc-
umentation reflect state-of-the-art technology.
We have compiled the texts and illustrations
as accurately as possible. Still, we are always
grateful for comments and suggestions regarding
potential errors within this documentation.
The information included in this manual may be
changed without prior notice.

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Contents
Contents
1. Important Notes about this Manual.............4
2. Function............................................................6
3. Safety Notes.....................................................9
3.1 Important Information for Operators and
Users .................................................................9
3.2 General Safety Notes .....................................9
3.3 Electrical Safety ............................................10
3.4 Specifications of the Lasers
Installed in the Supply Unit..........................13
3.5 Laser Safety ...................................................13
3.5.1 Safety notes for handling the laser ............13
4. Transport and Storage..................................15
4.1 Transport ........................................................15
4.2 Ambient Conditions.......................................15
4.3 Installation Location .....................................16
4.4 Disposal ..........................................................17
5. Overview of the Instrument.........................18
5.1 Mica 18
5.1.1 Status LED ......................................................18
5.2 Supply Unit .....................................................20
5.3 Incubator ........................................................21
5.4 Auto Immersion ............................................21
5.5 CORR objectives ...........................................21
5.6 Antivibration Table........................................22
6. Instrument Setup...........................................23
6.1 Switching on ..................................................23
6.2 Notes on Handling the Instrument Hood...23
7. Operation........................................................24
7.1 Starting the software....................................24
7.2 Software help.................................................24
7.3 Online Help .....................................................24
7.4 The User Interface ........................................25
7.5 First Experiment.............................................33
8. Care ...............................................................34
9. EU-Declaration of Conformity.....................35

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1. Important Notes about this Manual
(1.2)
→p. 20
Numbers in parentheses refer to illustrations; for
example, (1.2) refers to Fig. 1, item 2.
Numbers with a pointer arrow (for example
→page 20), point to a certain page of this man-
ual.
WARNING indicates a hazard with a medium
degree of risk that, if not avoided, can result
in death or serious injury.
CAUTION indicates a potentially hazardous
situation with a low degree of risk that, if not
avoided, may result in minor or moderate in-
jury.
Caution!
In this manual, additional safety notes are in-
dicated with the triangle symbol shown here,
and have a gray background.
Caution! The instrument and its accessories can
be damaged when operated incorrectly.
Text Symbols and Their Meanings:
Caution!
This User Manual is an essential component
of the product; it must be read carefully be-
fore the product is assembled, put into op-
eration or used, and must be kept for future
reference.
1. Important Notes about this Manual
This User Manual includes important instruc-
tions and information related to the operating
safety and maintenance of the instrument. .
!
WARNING
CAUTION

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1. Important Notes about this Manual
Warning of hazardous electrical voltage! Danger
of electric shock!
Warning of optical radiation! Never look directly
into the light beam! Wear safety goggles!
Warning of electromagnetic field
Warning of UV radiation! Wear safety goggles!
Warning of hazardous laser radiation – take ap-
propriate safety precautions!
Warning of hand injuries
Instructions for disposing of the instrument,
its accessories and consumables.
Ground terminal
Explanatory note
Item not contained in all equipment configura-
tions
Manufacturing date, e.g. 11 / 2011 for November
2011
China RoHS 50 years EFUP
(Environmentally friendly use period)
*
MM/YYYY

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2. Function
2. Function
The entire system of which this User Manual is
part is intended for research applications. The
Leica microscope Mica is an inverted light mi-
croscope and intended for use as a general lab
microscope for routine examinations of biologi-
cal specimens.
Directives of the European Community (EU Di-
rectives)
The microscope described here fulfills the re-
quirements of EU Directives 2014/35/EU concern-
ing the safety of electrical equipment, 2014/30/EU
concerning electromagnetic compatibility and
the Machinery Directive 2006/42/EC.
According to the VO1194/2012 regulation regard-
ing the implementation of the Ecodesign Direc-
tive 2009/125/EC, microscopes are considered
special products. The light sources in this instru-
ment are special lamps which are only suitable
for microscopes. They are not suitable for gener-
al lighting purposes or room lighting.
Reasonably Foreseeable Misuse
The following are prohibited:
• To use the system for any purpose
not in accordance with the Declara-
tion of Conformity (e.g. the use of IVD or
medical products in accordance with
EU regulation 2017/746 or 2017/45 )
• Operating the system in an inclined position
• Cleaning the system in a way other than speci-
fied in the manual
• Modifying the built-in safety circuits in the in-
strument
• Allowing unauthorized personnel to open the
instrument
• Using cables that Leica Microsystems CMS
GmbH has not provided or permitted
• Using combinations with non-Leica compo-
nents that go beyond the scope of the manual

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2. Function
Caution!
The manufacturer assumes no liability for
damage caused by, or any risks arising from,
using the microscope for other purposes than
those for which they are intended or not us-
ing them within the specifications of Leica
Microsystems CMS GmbH.
In such cases, the Declaration of Conformity
shall be invalid.
WARNING
Notes on Handling Laser Devices
In the standard design without additional la-
ser safety precautions, the microscopes are
not suitable for coupling laser radiation (such
as to the camera ports), since this radiation
poses a hazard for the user (eye injury in par-
ticular).
For use of the microscope with lasers, Leica
Microsystems CMS GmbH offers special mi-
croscope variants with additional safety fea-
tures. Laser couplings require corresponding
safety devices that have to be inspected and
installed by trained personnel.
For further information, please contact your
authorized Leica Microsystems CMS GmbH
representative.
WARNING
Due to the special sources of danger of the
laser system, only those persons may use the
system who have received special training in
working with the system, observing the spe-
cific hazards of a laser system.
CAUTION
Light sources pose a potential irradiation risk
(glare, UV radiation, IR radiation). Therefore,
lamps may only be operated in closed hous-
ing and in an installed condition.
Never look directly into the beam path
(blinding hazard).

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2. Function
CAUTION
If the hood to the specimen area is open
and the UV LED (365nm) is switched on with
maximum configured intensity, a distance
between the eye and light outlet interface on
the objective of <10cm may result in a hazard
to the eye.
No hazard for the eye exists in the normal use
case (distance between eye and objective
>10cm).
When changing specimens with the UV LED
(365nm) switched on at maximum intensity,
be sure not to exceed a maximum exposure
time of the skin (hands) of 60 minutes a day.
(actinic UV hazard)
We recommend dimming/deactivating all
LEDs before opening.

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3. Safety Notes
3. Safety Notes
3.1 Important Information for Operators and
Users
• The owner/operator and user of this product
are responsible for proper and safe operation
and safe maintenance of the system and for
following all applicable safety regulations.
• The owner/operator and user are fully liable
for all consequences resulting from the use of
the system for any purposes other than those
listed in the User Manual or the online help.
• The owner/operator and user are obligated to
perform and monitor suitable safety measures
(according to national regulations).
• The owner/operator and user are responsible
for observing the laser safety regulations ac-
cording to applicable country-specific regula-
tions.
• The owner/operator and user must ensure that
this laser product is started up and operated
only by persons who have been trained in the
use of the system and the potential dangers of
laser radiation.
• The owner/operator and user are fully liable
for all consequences resulting from the use of
the system if it is opened, improperly serviced
or repaired by persons other than authorized
Leica service representatives.
3.2 General Safety Notes
This protection class 1 instrument was built and
tested in accordance with
EN 61010-1/IEC 61010-1, Safety requirements for
electrical equipment for measurement, control
and laboratory use.
It also meets the requirements of EN / IEC 61326-
1, Electrical equipment for measurement, control
and laboratory use – EMC requirements.
The product has also been tested in accordance
with EN 60825-1 / IEC 60825-1 Safety of laser
products
Part 1: Equipment classifications and require-
ments checked.
The products were also tested in accordance
with IEC 62471 "Photobiological safety of lamps
and lamp systems" and are a risk of risk group
2 (medium risk). Observe the warning message
regarding optical radiation on the previous page.
Caution!
In order to maintain this condition and to en-
sure safe operation, the user must follow the
instructions and warnings contained in this
User Manual.
Also note the provided User Manuals of other
components (e.g. incubator, Mot.Corr, etc.).

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3. Safety Notes
The instruments and accessories described
in this manual have been safety-tested and
checked for possible hazards.
The responsible Leica Microsystems CMS
GmbH affiliate or the main plant in Wetzlar
must be consulted whenever the instrument
is altered, modified or used in conjunction
with non-Leica components that go beyond
the scope of this manual.
Unauthorized alterations to the device or
noncompliant use shall void all rights to any
warranty claims and product liability!
In addition to this manual, the safety notes
of the other manuals provided must be ob-
served!
The system may be set up, installed and put
into operation only by authorized Leica per-
sonnel!
Caution!
The microscope may be operated by autho-
rized personnel only.
3.3 Electrical Safety
General Specifications
In addition, observe the information about the
specifications in the other User Manuals provid-
ed!
Supply Unit
For indoor use only.
Supply voltage: 100 to 240 VAC
Frequency: 50 / 60 Hz
Power consumption: max. 220 VA
Fuses: 4 A, slow-blowing,
Breaking capacity H, 250 VAC,
Size: 5x20 mm
Ambient temperature: 15 – 35 °C
Relative humidity: 90 % up to 30 °C,
non-condensing
Protection class: I
Overvoltage category: II
Pollution degree: 2