NEW BRUNSWICK SCIENTIFIC I-2500KC User manual

I-2500KC
Digital Refrigerated
Incubator Shaker
with PROGRAMMABLE TIMER
MANUAL No. M1284-1054
Revision B
July 12, 2002
NEW BRUNSWICK SCIENTIFIC CO., INC.
BOX 4005 •44 TALMADGE ROAD •EDISON, NJ 08818-4005
Telephone: [phone] •1-800-631-5417
Fax: [phone] •Telex: 4753012 NBSCO
Internet: http://www.nbsc.com •E-mail: [email protected]

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I-2500KC •M1284-1054 User’s Guide

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New Brunswick Scientific Co., Inc. User’s Guide
CAUTION!
This equipment must be operated as described in this
manual. If operational guidelines are not followed,
equipment damage and personal injury can occur.
Please read the entire User’s Guide before attempting to
use this unit.

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New Brunswick Scientific Co., Inc. User’s Guide
Copyright Notice
New Brunswick Scientific Company, Inc.
Box 4005 44 Talmadge Road
Edison, New Jersey 08818-4005
©Copyright 2002 New Brunswick Scientific Co., Inc.
All Rights Reserved.
Reproduction, adaptation, or translation without prior written permission from
New Brunswick Scientific is prohibited.
Disclaimer Notice
New Brunswick Scientific Co., Inc. reserves the right to change
information in this document without notice. Updates to information in this
document reflect our commitment to continuing product development and
improvement.
Manual Conventions
NOTE:
CAUTION !
WARNING !
Bold Text in bold face type emphasizes key words or
phrases.
Caution messages appear before procedures
which, if caution is not observed, could result
in damage to the equipment.
Notes contain essential information that
deserves special attention.
Warning messages alert you to specific
procedures or practices which, if not followed
correctly, could result in serious personal
injury.

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New Brunswick Scientific Co., Inc. User’s Guide
I Series Shakers are warranted by New Brunswick Scientific Co., Inc.
for two years or 10,000 hours of actual shaker use, whichever comes later.
This warranty covers parts and labor for the entire machine, with the exception
of glassware and its contents. This warranty covers faulty components and
assembly, and our obligation under this warranty is limited to repairing or replacing
the shaker or part thereof which shall, within two years after date of shipment or
10,000 hours of operating time, prove to be defective after examination. This
warranty does not cover any loss of time, materials, biological or biochemical by-
products caused by any work interruption resulting from shaker failure, nor does it
extend to any I Series Shaker which has been subjected to misuse, neglect, accident
or improper installation or application. In addition, the warranty does not apply to
any I Series Shaker that has been repaired or altered outside the NBS factory without
prior authorization from New Brunswick Scientific. For a period of two years (or
more) after the shipment date, the I Series warranty will be in effect as long as
the shaker has not been in operation for a total of 10,000 hours. After the
10,000 hours of operating time have elapsed, the I Series warranty may
still be in effect, as long as the two-year minimum warranty period
has not been reached. Operating time is based on actual usage
of the shaker, as determined by the shaker’s internal
electronic clock. Any tampering or alteration of
the clock will void the 10,000-hour
warranty.
W A R R A N T Y

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New Brunswick Scientific Co., Inc. User’s Guide
TABLE OF CONTENTS
1OVERVIEW.....................................................................................................................................................11
1.1 GENERAL DESCRIPTION.............................................................................................................................11
2INSPECTION & VERIFICATION ...............................................................................................................13
2.1 INSPECTION ...............................................................................................................................................13
2.2 UNPACKING &VERIFICATION ..................................................................................................................13
3PREPARING THE LOCATION....................................................................................................................15
3.1 SPACE REQUIREMENTS..............................................................................................................................15
3.2 ENVIRONMENT ..........................................................................................................................................16
4FEATURES......................................................................................................................................................17
4.1 KEYPAD ....................................................................................................................................................18
4.2 ELECTRONIC TIMER...................................................................................................................................20
4.3 PLATFORM ASSEMBLIES............................................................................................................................23
4.4 QUICK CHANGE PLATFORM KIT................................................................................................................23
4.5 CONSTRUCTION.........................................................................................................................................23
4.6 REFRIGERATION SYSTEM...........................................................................................................................24
4.7 VENT.........................................................................................................................................................24
5GETTING STARTED.....................................................................................................................................25
5.1 LEVELING THE UNIT..................................................................................................................................25
5.2 ELECTRICAL CONNECTIONS ......................................................................................................................25
5.3 INSTALLATION OF PLATFORMS..................................................................................................................25
5.4 QUICK CHANGE PLATFORM OPTION..........................................................................................................26
5.5 INITIAL STARTUP.......................................................................................................................................27
6THE ELECTRONIC TIMER.........................................................................................................................29
6.1 SETTING THE ELECTRONIC TIMER .............................................................................................................29
6.2 ORDINARY TIMER OPERATION ..................................................................................................................30
6.3 SAMPLE PROGRAM....................................................................................................................................33
6.4 MULTIDAY OPERATION.............................................................................................................................37
6.5 CYCLIC OPERATION ..................................................................................................................................39
6.6 VIEWING PROGRAMS.................................................................................................................................41
6.7 DAY OVERRIDE.........................................................................................................................................44
6.8 CLEARING/CANCELING PROGRAMS...........................................................................................................48
7OPERATION ...................................................................................................................................................50
7.1 STARTING THE SHAKER.............................................................................................................................50
7.2 CONTINUOUS MODE..................................................................................................................................50
7.3 CHECKING ANY SETPOINT.........................................................................................................................51
7.4 ISERIES TIMER MODE..............................................................................................................................51
7.5 ALARM FUNCTIONS...................................................................................................................................52
7.6 PRIMARY TEMPERATURE SETPOINT...........................................................................................................53
7.7 PROGRAMMED TEMP MODE ......................................................................................................................53
7.8 PROGRAMMED SHAKE MODE ....................................................................................................................54
7.9 TEMPERATURE OFFSET CALIBRATION.......................................................................................................54
7.10 TOTAL RUNNING TIME ..............................................................................................................................55

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I-2500KC •M1284-1054 User’s Guide
7.11 MAINT INDICATOR ....................................................................................................................................56
7.12 POWER FAILURE........................................................................................................................................56
8PREVENTIVE MAINTENANCE..................................................................................................................58
8.1 CLEANING .................................................................................................................................................58
8.2 CONDENSER COIL......................................................................................................................................58
8.3 CHANGING FUSES......................................................................................................................................59
8.4 BELT REPLACEMENT .................................................................................................................................59
8.5 MOTOR ASSEMBLY REPLACEMENT ...........................................................................................................60
8.6 ESD PRECAUTIONS ...................................................................................................................................61
8.7 REPLACEMENT OF MAIN CONTROL BOARD...............................................................................................61
8.8 TEMPERATURE CONTROL BOARD REPLACEMENT .....................................................................................63
9SPECIFICATIONS..........................................................................................................................................66
10 REPLACEMENT PARTS & ACCESSORIES.............................................................................................68
10.1 REPLACEMENT PARTS DESCRIPTIONS AND PART NUMBERS......................................................................68
10.2 ACCESSORY DESCRIPTIONS &PART NUMBERS.........................................................................................69
11 DRAWINGS.....................................................................................................................................................72
11.1 CONTROL SCHEMATIC...............................................................................................................................72
11.2 LIST OF DRAWINGS ...................................................................................................................................78
12 INDEX...............................................................................................................................................................80

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New Brunswick Scientific Co., Inc. User’s Guide
1
1 O OV
VE ER RV VI IE EW W
1.1 General Description
The I-2500KC shaker with programmable timer incorporates a triple eccentric
counterbalanced drive to provide horizontal plane rotary motion in a 1inch (25.4 mm)
circular orbit. A Proportional/ Integral (PI) Microprocessor controller, with
instantaneous digital feedback, controls the speed over a range of 25-500 rpm. It also
provides temperature control over a range of 4.0°C to 60°C (at 20°C ambient). The
internal chamber is 34¾ inches (88.3 cm) wide, 22¼ inch (56.6 cm) deep, 19¼ inch (48.9
cm) above the platform and will accept flasks up to 6 liters. It is equipped with a foot-
pedal lid lift assist.
The I-2500KC is equipped with an integral refrigeration system which allows precise
temperature control at or below ambient temperature.
The shaker may be operated either continuously or in a timed mode via a programmable
timer for shaking periods of 0.1 hour to 99.9 hours.
The I-2500KC is equipped with audible and visible alarms that are activated when an
alarm condition exists, as follows:
•The end of a timed run
•Deviations of shaking speed or temperature outside of tolerance limits
A wide variety of platforms can be used with the I-2500KC. Dedicated platforms are
available for a variety of flask sizes. A Universal platform and test tube racks are
available. (See Section 10.1 for Accessories)

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New Brunswick Scientific Co., Inc. User’s Guide
2
2 I IN
NS SP PE EC CT TI IO ON N &
&
V
VE
ER RI IF FI IC CA AT TI IO ON N
2.1 Inspection
After you have received your order from Fisher Scientific, inspect the boxes carefully for
any damage that may have occurred during shipping. Report any damage to the carrier
and to your local Fisher sales representative. Do not discard any of the packing material.
2.2 Unpacking & Verification
Verify against your packing list that you have received the correct materials. Report any
errors or missing material to your local Fisher sales representative.

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New Brunswick Scientific Co., Inc. User’s Guide
3
3 P PR
RE EP PA AR RI IN NG G T TH HE E L
LO
OC CA AT TI IO ON N
3.1 Space Requirements
The surface where you place the I-2500KC should be smooth, level and sturdy.
It is essential that the instrument be situated in an area where there is sufficient space for
the shaker and service lines (see Figure 1 below).
Figure 1: Space Requirements
The outside dimensions of the I-2500KC are:
OUTSIDE DIMENSIONS
Width 45 inches 114 cm
Depth 28 inches 71 cm
Height 37 inches 94 cm
The effective surface area required for operation is:
REQUIRED SURFACE AREA
Width 48 inches 122 cm
Depth 30 inches 76 cm

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I-2500KC •M1284-1054 User’s Guide
3.2 Environment
The I-2500KC operates properly under the following conditions: 0°C - 35°C, 90%
humidity, non-condensing.

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New Brunswick Scientific Co., Inc. User’s Guide
4
4 F FE
EA AT TU UR RE ES S
Figure 2: I-2500KC Front Panel
KEYPAD
ELECTRONIC
TIMER
ON/OFF
SWITCH
CONTROL
PANEL
FOOT
PEDAL

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I-2500KC •M1284-1054 User’s Guide
4.1 Keypad Figure 3: Control Panel
4.1.1 LED Display
The digital display on the control panel is a three digit LED DISPLAY. During
normal shaker operation the display will indicate:
•Shaker status (on/off)
•Shaking speed
•Chamber temperature
•Setpoints
•Hours remaining (timed run)
•Lid open (cover open/shaker operation stops)
4.1.2 User Interface Keys
•START/STOP This key is used to start or stop the shaker. It will
also activate or stop the timer when a timed run is
desired.
•SELECT This key is used to change the displayed parameter.
•(UP), (DOWN) These keys are used to adjust the setpoint of a
displayed parameter up or down. They also allow
the user to enter the SET MODE for setpoint
changes.
FUNCTION
INDICATOR
S
STATUS
INDICATOR
S
LED
DISPLAY
USER INTERFACE
KEYS

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New Brunswick Scientific Co., Inc. User’s Guide
4.1.3 Status Indicators
Four status indicator lights are located to the left of the LED DISPLAY. They are:
•MAINT Remains lit after 10,000 hours of use. Accumulated
running time is internally monitored and may be
displayed as a guideline.
•SET Indicates that the shaker is in the SET MODE and
setpoints are being displayed and can be altered.
•TIME Indicates that the timer is in operation. The shaker
can be programmed to run for a preset time from
0.1 hour to 99.9 hours. The timer can be disengaged
without stopping an ongoing run.
•MUTE Indicates the status of the audible alarm. When
illuminated, the audible alarm is disabled.
4.1.4 Function Indicators
The four function indicator lights are located to the right of the LED display. They
indicate the current parameter being displayed.
•RPM
Revolutions per minute.
•HOURS
Time remaining when used in I SERIES TIMER MODE.
•° C
Used to display current incubator temperature and set primary temperature
setpoint.
•*
Used to display current incubator temperature and set alternate or secondary
temperature setpoint, which is used with the electronic timer.

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I-2500KC •M1284-1054 User’s Guide
4.2 Electronic Timer
The electronic timer is used to set programmed times to control the operation of the
shaker. PROG1 enables the unit to alternate between primary (°C setting on the keypad)
and secondary (*) temperature setpoints at preset intervals (cycles) or at specific times.
PROG2 controls the operation of the shaker. The timer controls speed by stopping and
starting the shaker at preset intervals (cycles) or at specific times.
Chapter 6 provides instructions for programming and using the electronic timer feature.
This feature allows the operator to leave a culture unattended over a weekend, for
example, and yet maintain a no-growth environment. By selecting the low temperature
setpoint from end of day Friday until a pre-determined time Sunday night or Monday
morning, the operator can ensure stasis while the culture is unsupervised. At the
operator-selected time, the temperature will rise to the maximum setpoint, encouraging
growth. (See Section 6.3 for details.)
Figure 4: Electronic Timer Detail
SHIFT
h
DAY/RESET
m
P1
P2
RUN
WRITE
CHECK
TIME ADJ
1
AUTO
OFF
AUTO
PROG 2
ON
COPY
CYCLE/PULSE CLR
12MAN
AUTO
TIMER
PULSE 12
MODE BOOT
AUTOMATIC
OPERATION
INDICATOR
SHIFT KEY
SET/RESET
KEY
h KEY
WRITE KEY
CHECK KEY
m KEY
MODE
SWITCH
OUTPUT
ON/OFF
SWITCH
CLR
KEY
COPY
KEY
CYCLE/PULSE
KEY
DRAWER
DRAWER COVER
(OPEN)