NEW BRUNSWICK SCIENTIFIC Excella E-24 User manual

Excella E-24/24R
Benchtop
Incubator Shakers
MANUAL NO: M1352-0050
Revision A
March 27, 2006
NEW BRUNSWICK SCIENTIFIC CO., INC.
BOX 4005 •44 TALMADGE ROAD •EDISON, NJ 08818-4005
Telephone: [phone] •1-800-631-5417
Fax: [phone] •Telex: 4753012 NBSCO
Internet: http://www.nbsc.com •E-mail: [email protected]

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Excella E-24/24R Incubator Shakers M1352-0050 User’s Guide

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New Brunswick Scientific Co., Inc. User’s Guide
INTERNATIONAL OFFICES:
BELGIUM
New Brunswick Scientific NV-SA
Stationsstraat 180/4
3110 Rotselaar
België/Belgique
Tel: [phone]
Fax: [phone]
E-mail: [email protected]
GERMANY
New Brunswick Scientific GmbH
In Der Au 14
D-72622 Nürtingen
Deutschland
Tel: [phone]
Fax: [phone]
E-mail: [email protected]
CHINA
New Brunswick Scientific Co., Inc.
A903, 904 Yin Hai Building
No. 250, Cao Xi Road
Shanghai 200235, P.R. China
Tel: [phone] or 5966
Fax: [phone]
E-mail: [email protected]
THE NETHERLANDS
New Brunswick Scientific BV
Kerkenbos 1101, 6546 BC Nijmegen
P.O Box 6826, 6503 GH Nijmegen
Nederland
Tel: [phone]
Fax: [phone]
E-mail: [email protected]
FRANCE
New Brunswick Scientific SARL
12-14, Rond Point des Champs Elysées
75008 Paris
France
Tel: [phone]
Fax: [phone]
E-mail: [email protected]
UNITED KINGDOM
New Brunswick Scientific (UK) Ltd.
17 Alban Park
St. Albans, Herts. AL4 0JJ
United Kingdom
Tel: [phone] or 0800 581331
Fax: [phone]
E-mail: [email protected]
Web: www.nbsuk.co.uk

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Excella E-24/24R Incubator Shakers M1352-0050 User’s Guide
CAUTION!
This equipment must be operated as described in this manual. If
operational guidelines are not followed, equipment damage and personal
injury can occur.
Please read the entire User’s Guide before attempting to use this unit.
Do not use this equipment in a hazardous atmosphere or with hazardous
materials for which the equipment was not designed.
New Brunswick Scientific Co., Inc. (NBS) is not responsible for any
damage to this equipment that may result from the use of an accessory
not manufactured by NBS.

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New Brunswick Scientific Co., Inc. User’s Guide
Copyright Notice
New Brunswick Scientific Company, Inc.
Box 4005 44 Talmadge Road
Edison, New Jersey 08818-4005
©Copyright 2006 New Brunswick Scientific Co., Inc.
All Rights Reserved.
Reproduction, adaptation, or translation without prior written permission from
New Brunswick Scientific is prohibited.
Disclaimer Notice
New Brunswick Scientific Co., Inc. reserves the right to change
information in this document without notice. Updates to information in this
document reflect our commitment to continuing product development and
improvement.
Manual Conventions
NOTE: Notes contain essential information that deserves
special attention.
CAUTION!
CAUTION messages appear before procedures
which, if caution is not observed, could result in
damage to the equipment.
WARNING! WARNING messages alert you to specific
procedures or practices which, if not followed
correctly, could result in serious personal injury.
Bold Text in bold face type emphasizes key words or
phrases.
Biohazard-related messages.
CRUSH WARNING!
Crush Warning messages alert you to specific
procedures or practices regarding heavy objects
which, if not followed correctly, could result in
serious personal injury .

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Excella E-24/24R Incubator Shakers M1352-0050 User’s Guide
Every Instrument manufactured by the
New Brunswick Scientific Co., Inc. is warranted to be free
from defects in material and workmanship. This apparatus, with
the exception of glassware, lamps and electrodes (where supplied),
is warranted against faulty components and assembly for 2 years
in the United States & Canada and for 1 year elsewhere. Our
obligation under this warranty is limited to repairing or replacing
the instrument or part thereof, which shall, upon our examination,
prove to be defective. The warranty period begins at the date of
shipment. This warranty does not extend to any NBS products
which have been subjected to misuse, neglect, accident or
improper installation or application; nor shall it extend to
products which have been repaired or altered outside the
NBS factory without prior authorization from the
New Brunswick Scientific Co., Inc.
W A R R A N T Y

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New Brunswick Scientific Co., Inc. User’s Guide
TABLE OF CONTENTS
1OVERVIEW.........................................................................................................................9
2INSPECTION & UNPACKING OF EQUIPMENT ......................................................11
2.1 INSPECTION OF BOXES ..................................................................................................11
2.2 PACKING LIST VERIFICATION .......................................................................................11
2.3 UNPACKING OF EQUIPMENT..........................................................................................11
2.4 OUT OF BOX CONCERNS ...............................................................................................12
2.5 WARRANTY REGISTRATION ..........................................................................................12
3PREPARING THE LOCATION......................................................................................13
3.1 PHYSICAL LOCATION ....................................................................................................13
3.2 ENVIRONMENT..............................................................................................................13
3.3 ELECTRICAL REQUIREMENTS........................................................................................13
3.4 SPACE REQUIREMENTS .................................................................................................14
4INSTALLATION...............................................................................................................15
4.1 INSTALLATION OF PLATFORM .......................................................................................15
4.2 FLASK CLAMP INSTALLATION.......................................................................................16
4.3 ELECTRICAL CONNECTIONS..........................................................................................17
5FEATURES........................................................................................................................18
5.1 CONTROL PANEL...........................................................................................................18
5.1.1 Keypad .................................................................................................................18
5.1.2 User Interface Keys .............................................................................................19
5.1.3 LED Display.........................................................................................................19
5.1.4 Status Indicators..................................................................................................20
5.1.5 Function Indicators..............................................................................................20
6OPERATION .....................................................................................................................22
6.1 ELECTRICAL CONNECTIONS..........................................................................................22
6.2 STARTING THE SHAKER.................................................................................................22
6.3 CONTINUOUS (UNTIMED) RUN......................................................................................23
6.4 CHECKING ANY SETPOINT ............................................................................................23
6.5 TIMED FUNCTIONS........................................................................................................23
6.6 ALARM FUNCTIONS.......................................................................................................24
6.7 TEMPERATURE SETPOINT..............................................................................................24
6.8 TEMPERATURE OFFSET CALIBRATION ..........................................................................25
6.9 POWER FAILURE ...........................................................................................................26
6.10 SPEED CALIBRATION.....................................................................................................26
7MAINTENANCE...............................................................................................................27
7.1 ROUTINE MAINTENANCE ..............................................................................................27
7.2 CLEANING EXTERNAL & INTERNAL SURFACES.............................................................27

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Excella E-24/24R Incubator Shakers M1352-0050 User’s Guide
7.3 BIOHAZARD DECONTAMINATION..................................................................................27
8SERVICE............................................................................................................................29
8.1 TROUBLESHOOTING ......................................................................................................29
8.2 PRODUCT RETURNS.......................................................................................................30
8.3 OPENING THE SERVICE COMPARTMENT........................................................................30
8.4 FUSE REPLACEMENT.....................................................................................................31
8.5 BELT REPLACEMENT OR ADJUSTMENT .........................................................................33
8.6 REPLACEMENT PARTS...................................................................................................35
9ACCESSORIES .................................................................................................................36
9.1.1 Platforms..............................................................................................................36
9.1.2 Flask Clamps for Universal Platforms................................................................36
9.1.3 Replacement Clamp Hardware Kits ....................................................................37
9.1.4 Test Tube Racks & Other Accessories.................................................................37
10 DRAWINGS & TABLES..............................................................................................39
10.1 SCHEMATICS.................................................................................................................39
10.2 LIST OF DRAWINGS.......................................................................................................44
10.3 LIST OF TABLES ............................................................................................................45
11 SPECIFICATIONS........................................................................................................46
11.1 CERTIFICATIONS ...........................................................................................................47
12 APPENDIX: PRODUCT RETURNS..........................................................................49
12.1 RETURN PROCEDURE ....................................................................................................49
12.2 RETURN AUTHORIZATION AND DECONTAMINATION CERTIFICATE ...............................49
13 INDEX.............................................................................................................................51

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New Brunswick Scientific Co., Inc. User’s Guide
1
1 O OV
VE ER RV VI IE EW W
The Excella E-24/24R Benchtop Incubator Shakers use a UniCentric™ counter-balanced drive
mechanism. They provide horizontal plane rotary motion in a ¾-inch (1.9 cm) diameter circular
orbit. A Proportional/Integral (PI) microprocessor controls the speed and temperature over the
entire range.
The incubated and refrigerated E-24R operates from 15ºC below ambient (with a minimum
setpoint of 4ºC) to 60°C, and the incubated E-24 from 7ºC above ambient to 60ºC. Both these
ranges depend on relative humidity and other ambient factors. Ambient temperature is measured
one meter from the front of the unit.
Erlenmeyer flasks, 2.8-liter Fernbach flasks, and a wide variety of tubes and plates can be
accommodated using the New Brunswick Scientific shaker accessories described in Section 9.
The E-24/24R may be operated in the following ways:
• Continuously: at a set speed and temperature, until user intervention.
• In a timed mode: run at a set speed, time and temperature for a period of up to 99.9
hours, after which the shaker automatically shuts off, while the temperature is maintained
at its setpoint.
For safe operation, the E-24/24R shakers are designed with a safety switch that automatically
stops the shaker mechanism when the lid is opened.
Both the E-24 and the E-24R are equipped with visual and audible alarms that alert the user to
the following conditions:
• The end of a timed run
• Deviations from speed setpoint (5 minutes after hood is closed)
• Deviations from temperature setpoint (5 minutes after hood is closed)
• Power failure
• Lid (hood) open
To accommodate customer needs, a wide variety of platforms can be used with the Excella
E-24/24R:
• Universal platforms are the most flexible, providing hole patterns for flask clamps,
test tube racks and other accessories.
• Dedicated platforms are supplied with flask clamps attached; they are designed solely
and expressly for this purpose.

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Excella E-24/24R Incubator Shakers M1352-0050 User’s Guide
• Test tube racks, microplate holders, and test tube rack holders are also available (a
universal platform is needed for all test tube racks and holders).
For further information on these accessories, see Section 9.

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New Brunswick Scientific Co., Inc. User’s Guide
2
2 I IN
NS SP PE EC CT TI IO ON N &
& U UN
NP PA AC CK KI IN NG G O OF F
E
EQ
QU UI IP PM ME EN NT T
2.1 Inspection of Boxes
After you receive your order, inspect the boxes carefully for any damage that may have
occurred during shipping. Report any damage immediately to the carrier and to your
local sales representative.
2.2 Packing List Verification
Verify against your packing list that you have received all of the correct materials.
2.3 Unpacking of Equipment
WARNING!
Do not attempt to lift the Excella E-24/24R by yourself. Always ask for
assistance or use a lifter or other suitable equipment when raising or
handling the unit.
CRUSH WARNING!
It is preferable to lift the Excella E-24/24R from the sides of the unit,
but if you lift it from the front and back, be sure to use the finger holes
in the back lip (toward the bottom of the unit). If these finger holes are
not used, the back lip can pinch fingers and hands.
Upon unpacking the unit, inspect it carefully for any damage that may have occurred
during transit. Report any apparent damage to the carrier and to your sales
representative. Save the crate and packing materials.
NOTE:
Use of the Excella E-24/24R Shakers requires a platform, which is a separate
item. See the Available Platforms list in Section 9.1.1.

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Excella E-24/24R Incubator Shakers M1352-0050 User’s Guide
2.4 Out of Box Concerns
If any part of your order was damaged during shipping, is missing pieces, or fails to
operate properly, please contact your sales representative and also fill out Customer
Satisfaction Form 6300, included with your equipment, and return it by fax.
2.5 Warranty Registration
Please complete and return your warranty card or register electronically at our Website:
www.nbsc.com

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New Brunswick Scientific Co., Inc. User’s Guide
3
3 P PR
RE EP PA AR RI IN NG G T TH HE E L
LO
OC CA AT TI IO ON N
3.1 Physical Location
WARNING!
Do not attempt to lift the Excella E-24/24R by yourself. Always ask for
assistance or use a lifter or other suitable equipment when raising or
handling the unit.
CRUSH WARNING!
It is preferable to lift the Excella E-24/24R from the sides of the unit,
but if you lift it from the front and back, be sure to use the finger holes
in the back lip (toward the bottom of the unit). If these finger holes are
not used, the back lip can pinch fingers and hands.
The surface where you place the E-24/24R should be smooth, level and sturdy, and must
be able to accommodate 200 pounds.
3.2 Environment
The shaker is designed to operate optimally in the following ambient conditions:
• 10º to 35°C
• 20 to 80% Relative Humidity (non-condensing)
3.3 Electrical Requirements
The E-24/24R can be equipped to run on:
 100 Volts, 50/60 Hz, 1500 VA maximum
 120 Volts, 60 Hz, 1500 VA maximum
 230 Volts, 50 Hz, 1500 VA maximum
In all cases, voltage variations must not exceed ±10%.

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Excella E-24/24R Incubator Shakers M1352-0050 User’s Guide
3.4 Space Requirements
It is essential that the shaker be situated in an area where there is sufficient space for the
unit and its service lines (see Figure 1).
Figure 1: Space Requirements
The dimensions of the Excella E-24/24R are:
Width 22 inches 55.8 cm
Depth 30 inches 76.2 cm
Height 24 1/16 inches 61.1 cm
Height with lid open 40½ inches 101.9 cm
NOTE:
Be sure to allow at least three inches (7.6 cm) around shaker for ventilation,
access to power cord (rear panel), and access to power switch and RS-232
port (right side).
The effective surface area required for operation is:
Width 24 inches 61 cm
Depth 30 inches 76.2 cm
Allow 27 inches
of width
to provide clearance
Allow 33 inches of depth
to provide clearance
Allow 42 inches of
height to provide
clearance (for
opening the lid)

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New Brunswick Scientific Co., Inc. User’s Guide
4
4 I IN
NS ST TA AL LL LA AT TI IO ON N
4.1 Installation of Platform
NOTE:
There are two small plastic straps that hold the bearing housing in place for
shipping. The straps must be removed from the unit.
Prior to use, a platform must be installed on the unit. The unit is shipped with four
Allen head PLATFORM SCREWS installed in the SUBPLATFORM of the BEARING
HOUSING. These screws must be removed and set aside for use before a platform can be
installed:
Figure 2: Platform Screw Locations
1. Using the 7/32-inch hex wrench provided, remove the four Allen head platform
screws from the subplatform. Set them aside.
2. Place the selected PLATFORM on the subplatform. Align the mounting holes of the
platform with the platform screw locations in the subplatform.
3. Insert the four Allen head platform screws previously removed and set aside. Tighten
them with the 7/32-inch hex wrench provided to secure the platform.
SUBPLATFORM

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Excella E-24/24R Incubator Shakers M1352-0050 User’s Guide
4.2 Flask Clamp Installation
Flask clamps purchased for use with universal platforms (see Section 9.1.1) require
installation. Clamps are installed by securing the base of the clamp to the platform with
the correct type and number of screws. All clamps are shipped complete with hardware.
NOTE:
The Excella E-24 and E-24R platforms require 10-24 x 5/16-inch screws,
which are the smaller Phillips head screws supplied, to fasten flask clamps.
Clamps for 2- and 2.8-liter flasks are shipped with an additional girdle to keep the flasks
in place. The girdle is an assembly of springs and sections of rubber tubing. One girdle
is already in place on the clamp, the other is packed separately. To install these double
girdle clamps:
1. Place the clamp on the platform, aligning its mounting holes with holes on the
platform. Secure the clamp in place using the flat Phillips head screws provided
(#S2116-3051, 10-24 x 5/16-inch). Use Figure 3b to help you identify the proper
screws, as three different types of screws are shipped with the clamps.
2. With the first girdle in place, as delivered, on the upper part of the clamp body (see
Figure 3a), insert an empty flask into the clamp.
3. After making sure the sections of tubing are located between the clamp legs, roll the
first girdle down the legs of the clamp as far as it can go. The tubing sections will
rest against the platform, and the springs will be under the clamp base.
4. Place the second girdle around the upper portion of clamp body (just as the first
girdle was initially). Make sure that its spring sections rest against the clamp legs,
while its rubber tubing sections sit against the flask, in between the clamp legs.
Figure 3a: Double Girdle Clamp Installation
UPPER GIRDLE
WITH GIRDLE
TUBES
LOWER
GIRDLE WITH
GIRDLE TUBES
PLATFORM
CLAMP
MOUNTING
HOLES (5)
CLAMP BODY
(LEGS AND BASE)

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New Brunswick Scientific Co., Inc. User’s Guide
Figure 3b: Clamp Fastener
9
NOTE:
The upper girdle secures the flask within the clamp, and the bottom girdle
keeps the flask from spinning.
NBS flask clamps are used on a variety of shaker platforms. Flat head screws of
different lengths and thread pitch are used to secure the clamp. With reference to Figure
3b above, select the appropriate screws and set the others aside.
NOTE:
One-liter and larger flask clamps are fastened with 5 screws.
4.3 Electrical Connections
CAUTION!
Before making electrical connections, be sure to check the following:
1. If you have not already done so, check that the voltage and frequency of your unit are
compatible with your electric supply.
2. Remove the caution label from the rear of the unit.
3. Set the circuit breaker on the right side of the unit to the OFF position.
ONLY THEN:
4. Plug the power cord into a grounded electrical outlet.
CAUTION!
A grounded electrical outlet is necessary for the safe operation of this
instrument.

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Excella E-24/24R Incubator Shakers M1352-0050 User’s Guide
5
5 F FE
EA AT TU UR RE ES S
5.1 Control Panel
The front panel (see Figure 4) of the Excella E-24/24R shakers provides easy access to
the control panel, which consists of the keypad, display and indicator lights. The ON/OFF
switch is conveniently located on the right side of the unit, as you face the controls.
Figure 4: Control Panel
5.1.1 Keypad
The keypad (see close-up in Figure 4a on the following page) is the operator’s
command center for the shaker. In one convenient location are grouped the user
interface keys, the LED display, the status indicator lights and the function
indicator lights.
ON/OFF
SWITCH
KEYPAD (see Sections 5.1.1 & 5.1.2)
INDICATORS (see Sections 5.1.4 & 5.1.5)LED DISPLAY
(see Section 5.1.3 )

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New Brunswick Scientific Co., Inc. User’s Guide
Figure 4a: Keypad
5.1.2 User Interface Keys
• START/STOP This key is used to start or stop the shaker. It will
also activate or stop the timer when a timed run is
desired.
• SELECT This key is used to change the displayed parameter.
• V(UP), W(DOWN) These keys are used to adjust the setpoint of a
displayed parameter up or down. They also allow
the user to enter the SET MODE for setpoint
changes.
5.1.3 LED Display
The digital display on the control panel is a three-digit LED DISPLAY. During
normal shaker operation, the display will indicate:
• Shaker status (On/Off)
• Shaking speed
• Chamber temperature
• Setpoints
• Hours remaining (in a timed run)
• Lid open (“LID”)
USER INTERFACE
KEYS
LED
DISPLAY
STATUS
INDICATORS
FUNCTION
INDICATORS

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Excella E-24/24R Incubator Shakers M1352-0050 User’s Guide
5.1.4 Status Indicators
Four status indicator lights are located to the left of the LED DISPLAY. They are:
• SET Indicates that the shaker is in the SET MODE,
when setpoints are being displayed and can be
altered. This is activated by the SELECT key
or by pressing the V(UP) or W(DOWN) arrow.
• POWER Illuminates and blinks during power up or if
power is interrupted during a run. Press the
SELECT key and change to another function to
turn off this indicator.
• HEAT Illuminates to indicate that the heater is on.
• TIME Indicates that the timer is in operation. The
shaker can be programmed to run for a preset
time from 0.1 to 99.9 hours. The timer can be
disengaged without stopping an ongoing run.
5.1.5 Function Indicators
Four function indicator lights are located to the right of the LED DISPLAY. They indicate
the current parameter(s) being displayed:
• °C Interior chamber temperature. Can be set from
4°C to 60°C, when in SET MODE, using the
V(UP) or W(DOWN) arrow key. It indexes at
0.1°C increments unless the key is pressed for
4 seconds, after which it indexes more rapidly.
• RPM Revolutions per minute. When in SET MODE,
use the V(UP) or W(DOWN) arrow key to
change the speed. It indexes at 1 RPM
increments unless the key is pressed for 4
seconds, after which it indexes more rapidly.