NEW BRUNSWICK SCIENTIFIC AF 2000 User manual

AF 2000
Anti–Foam Control Module
MANUAL NO: M1230–0053
Rev E
March 4, 1998
NEW BRUNSWICK SCIENTIFIC CO., INC.
BOX 4005 . 44 TALMADGE ROAD . EDISON, NJ 08818-4005
TELEPHONE: . [phone] . 800-631-5417 . FAX: [phone]
TELEX: 4753012 NBSCO . INTERNET: http://www.nbsc.com
E-MAIL: [email protected]
UNITED KINGDOM THE NETHERLANDS BELGIUM GERMANY
New Brunswick Scientific (UK) Limited New Brunswick Scientific Benelux B.V. New Brunswick Scientific NV / SA New Brunswick Scientific GMBH
Edison House–163 Dixons Hill Road P.O Box 6826 ‘t Veldeke, 1 B-1970 In Der Au 14
North Mymms Hatfield Hertfordshire 6503 GH Nijmegen Wezembeek-OPPEM D-72622 Nürtingen
AL9 7JE United Kingdom Holland België/Belgique Deutschland
Tel: [phone] Tel: [phone] Tel: [phone] Tel: [phone]
Fax: [phone] Fax: [phone] Fax: [phone] Fax: [phone]
Telex: 94018208 NBSC G

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CAUTION !CAUTION !
Operate the AF 2000 as described in this manual.Operate the AF 2000 as described in this manual.
If Operational Guidelines are not followed, equipment damage can
occur. Please read the entire User’s Guide before attempting to use
the AF 2000.
If Operational Guidelines are not followed, equipment damage can
occur. Please read the entire User’s Guide before attempting to use
the AF 2000.
AF 2000 User’s GuideAF 2000 User’s Guide

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Copyright Notice
New Brunswick Scientific Company, Inc.
Box 4005 44 Talmadge Road
Edison, New Jersey 08818–4005
© Copyright 1995 New Brunswick Scientific Co., Inc.
All Right Reserved.
Reproduction, adaptation or translation without prior written permission from
New Brunswick Scientific is prohibited.
Disclaimer Notice
New Brunswick Scientific Company Inc., reserves the right to change information
in this document without notice. Updates to this information reflect our
commitment to continuing product development and improvement.
Manual Conventions
#NOTE: Notes contain important and useful information separate from text.
Caution messages appear before procedures which, if not
observed, CAUTION ! could result in damage to the equipment.
Warning messages alert you to specific procedures or practices
WARNING ! which, if not followed correctly, could result in serious personal
injury.
Bold Text in Bold face type emphasizes key words or phrases.
New Brunswick Scientific Co., Inc.

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Every Instrument manufactured by the New Brunswick Scientific
Co., Inc. is warranted to be free from defects in material and work-
manship. This apparatus with the exception of glassware, lamps and
electrodes (where supplied), is warranted for 1 year against faulty com-
ponents and assembly and our obligation under this warranty is limited
to repairing or replacing the instrument or part thereof, which shall
within 1 year after date of shipment, prove to be defective after our
examination. This warranty does not extend to any NBS products
which have been subjected to misuse, neglect, accident or improper
installation or application; nor shall it extend to products which
have been repaired or altered outside the NBS factory without
prior authorization from New Brunswick Scientific Co., Inc.
W A R R A N T Y
AF 2000 User’s Guide

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CONTENTS
1. OVERVIEW................................................................................................................. 8
1.1 SPECIFICATIONS......................................................................................................9
2. INSPECTION, VERIFICATION & UNPACKING OF EQUIPMENT............................. 9
2.1 INSPECTION OF BOXES............................................................................................. 9
2.2 PACKING LIST VERIFICATION..................................................................................... 9
3. PREPARING THE LOCATION................................................................................. 10
3.1 PHYSICAL LOCATION..............................................................................................10
3.2 ENVIRONMENT....................................................................................................... 10
4. UNPACKING & INSPECTION.................................................................................. 11
4.1 UNPACKING THE EQUIPMENT..................................................................................11
4.2 INSPECTION OF EQUIPMENT .................................................................................... 11
5. ANTI–FOAM MODULE FEATURES......................................................................... 13
6. GETTING STARTED ................................................................................................ 16
6.1 STACKING CONTROL MODULES............................................................................... 17
6.2 ANTI–FOAM MODULE INTERCONNECTING CABLE CONNECTION.................................. 18
7. PREPARATION BEFORE THE AUTOCLAVE......................................................... 19
7.1 VESSEL HEADPLATE ASSEMBLY.............................................................................. 19
7.2 PREPARING THE CONDUCTANCE PROBE FOR THE AUTOCLAVE................................... 23
New Brunswick Scientific Co., Inc.

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8. FERMENTATION SETUP......................................................................................... 24
8.1 CONDUCTANCE PROBE LEAD CONNECTION.............................................................. 24
8.2 SENSITIVITY ADJUSTMENT...................................................................................... 24
8.3 ANTI–FOAM CONTROL ...........................................................................................25
8.4 HIGH FOAM CONTROL............................................................................................ 25
8.5 DISPLAYING THE CURRENT VALUES......................................................................... 27
9. PREVENTIVE MAINTENANCE................................................................................ 27
9.1 CLEANING PROCEDURES........................................................................................ 28
9.2 FUSE REPLACEMENT.............................................................................................. 28
10. TROUBLESHOOTING............................................................................................ 30
11. REPLACEMENT PARTS AND ACCESSORY INFORMATION ............................. 31
11.1 REPLACEMENT PARTS DESCRIPTIONS AND PART NUMBERS..................................... 31
11.2 ACCESSORY DESCRIPTIONS AND CONDUCTANCE PROBES PART NUMBERS ..............31
APPENDIX A Control Schematic–AF 2000.................................................................. 32
INDEX...........................................................................................................................33
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New Brunswick Scientific Co., Inc.

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1. OVERVIEW
The New Brunswick Scientific's AF 2000 Anti–Foam Module is an optional unit that
connects to the BioFlo 2000 Fermentor. The Anti–Foam Module regulates the operation
of a self contained feed pump that delivers anti–foam agent and limits the agitation rate
when high foam condition occur.
To operate the Anti–Foam Module you must purchase a Conductance Probe and
Conductance Probe Leads separately. The length of the Conductance Probe
should be selected to match the size of your fermentation vessel.
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1.1 SPECIFICATIONS
AF 2000
Dimensions 9½″x 10½″x 3 5/16″
(241 mm x 267 mm x 84 mm)
Electrical 24 Volts AC–for Control Module
100–240 Volts–for BioFlo 2000 Fermentor
Pump 0–6.35 mL/min.
SetPoint Range 0–100%
Pump Head Watson Marlow 101
Weight 7 lbs. (3 kg)
2. INSPECTION, VERIFICATION & UNPACKING OF EQUIPMENT
2.1 INSPECTION OF BOXES
After you have received your order from New Brunswick Scientific, inspect the
boxes carefully for any damage that may have occurred during shipping. Report
any damage to the carrier and to your local NBS Sales Order Department.
[phone] / 800-631-5417.
2.2 PACKING LIST VERIFICATION
Verify on your NBS packing list that you have received the correct materials.
New Brunswick Scientific Co., Inc.

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3. PREPARING THE LOCATION
3.1 PHYSICAL LOCATION
The surface where you place the Anti–Foam Module should be smooth, level and
sturdy. All Control Modules should be stacked to the immediate right or left of
the BioFlo 2000 Fermentor. Ensure that there is enough space around the back
and the front of the Anti–Foam Module for proper operation.
CAUTION !
When stacking additional Control Modules, the pH 2000 should
always be on the bottom. When the pH 2000 is on the bottom and
an acid or base spill occurs, the spill will not damage
additional Modules.
3.2 ENVIRONMENT
The Anti–Foam Module operates properly under the following conditions:
•ambient temperature range 10°C–35°C
•relative humidity up to 80% non-condensing
•voltage fluctuation ±10%
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4. UNPACKING & INSPECTION
4.1 UNPACKING THE EQUIPMENT
Save all packing materials and the User’s Guide. If any part of your order was
damaged during shipping, is missing any pieces or fails to operate, please fill out
the Customer Satisfaction Form 6300 and return by fax or mail. You can also call
the New Brunswick Scientific Service Department at
[phone] / 800-237-2298.
To properly unpack the AF 2000 perform the following steps:
4.1.1 Open the top of the box.
4.1.2 Remove the START UP KIT from the package.
4.1.3 Remove the Anti–Foam Module from the box.
4.2 INSPECTION OF EQUIPMENT
Verify that you have received the following equipment :
NEW BRUNSWICK SCIENTIFIC
Edison NJ U.S.A.
AF 2000 CONTROLLER
PROBE
GRND
PROBE
OFF
AUTO
PRIME
FOAM
HIGH
SENS
SENS
ANTI–FOAM MODULE
New Brunswick Scientific Co., Inc.

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SPARE FUSE
GROUND POST SCREW
Anti–Foam
Module
User’s Guide
ANTI–FOAM MODULE USER’S GUIDE
ANTI–FOAM MODULE
INTERCONNECTING CABLE
(15 PIN)
4 SUPPORT PILLARS
CONDUCTANCE PROBE HEADPLATE FITTING
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5. ANTI–FOAM MODULE FEATURES
•ANTI–FOAM MODULE INTERCONNECTING Supplies communications and
CABLE power between the BioFlo 2000
Fermentor and Anti–Foam
Module
•SUPPORT PILLARS Four stainless steel screw-in pins
provide stability for stacking
additional Control Modules on
top of the Anti–Foam Module
•CONDUCTANCE PROBE HEADPLATE FITTING Used on the VESSEL
HEADPLATE–Properly fits and
seals the CONDUCTANCE PROBE
into the VESSEL HEADPLATE
•ON / OFF ROCKER POWER SWITCH Located on the lower left side of
the front panel–Supplies power
to the Anti–Foam Module
•FOAM PROBE INPUT Located on front left side of the
Anti–Foam Module–Connector
for CONDUCTANCE PROBE
Lead
•HIGH FOAM PROBE INPUT Located on the left side of the
front panel– Connector for
CONDUCTANCE PROBE LEAD
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•GROUND (GRND) INPUT CONNECTOR Located on the left side of the
front panel–Connector for the
GROUND LEAD
•FOAM SENSITIVITY (SENS) ADJUSTMENT Located on the upper left side of
the front panel–Adjusts for the
conductivity at which the
CONDUCTANCE PROBE and the
ANTI–FOAM Module respond to
the foam probe being in contact
with foam
•HIGH FOAM SENSITIVITY (SENS) ADJUSTMENT Located on the lower left side of
the front panel–Adjusts for the
conductivity at which the and the
CONDUCTANCE PROBE and the
ANTI–FOAM Module respond to
the high foam probe being in
contact with foam
•ANTI–FOAM PUMP MODE SWITCH Located on the left of the Anti–
Foam Pump Head–Selects Pump
operating modes
•ANTI–FOAM PUMP Located on the right front panel–
Adds Anti–Foam agent to the
fermentation while the
CONDUCTANCE PROBE is in
contact with foam–Addition rate
is controlled by the Bio Flo 2000
Fermentor
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•15 PIN POWER /CONTROL CONNECTOR Located on the back panel of the
Anti–Foam Module–Connects
power and communications from
BioFlo 2000 Fermentor to the
Anti–Foam Module
•2 AMP FUSE RECEPTACLE On the back panel of the Anti–
Foam Module–Holds the
working fuse for the Module
Anti–Foam Module
Front Panel
Foam Sensitivity (SENS) Adjustment
Foam Probe Input
On / Off Rocker Power Switch
NEW BRUNSWICK SCIENTIFIC
Edison NJ U.S.A.
AF 2000 CONTROLLER
Anti–Foam Pump
Mode Switch
PROBE
GRND
PROBE
FOAM SENS
OFF
AUTO
PRIME
HIGH SENS
High Foam Probe Input
Ground (GRND) Connector
Anti–Foam Pump
High Foam Sensitivity (SENS) Adjustment
New Brunswick Scientific Co., Inc.

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Anti–Foam Module
Back Panel
AF 2000
6. GETTING STARTED
Fuse Receptacle
2 AMP
(T)
FUSE
15 Pin Power / Control Connector
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6.1 STACKING CONTROL MODULES
If you are stacking additional Modules onto the Anti–Foam Module, perform the
following steps:
6.1.1 Use a Phillips Head (+) screwdriver and remove the four Phillips Head
screws from the top of the Anti–Foam Module.
6.1.2 Screw the four SUPPORT PILLARS into the four holes on the top of the
Anti–Foam Module.
6.1.3 Verify that the SUPPORT PILLARS are secure.
6.1.4 Align any additional Control Module on top of the Anti–Foam Module.
Verify that the rubber grommets on the bottom of the additional
Control Module match up with the SUPPORT PILLARS on the top of the
Anti–Foam Module.
6.1.5 Push the additional Control Module down, and verify that the Module
sits securely on the Anti–Foam Module.
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6.2 ANTI–FOAM MODULE INTERCONNECTING CABLE CONNECTION
Before you can run any experiments you must connect the INTERCONNECTING
CABLE. This cable supplies communications between the Anti–Foam Module
and the BioFlo 2000 Fermentor. Perform the following steps to make the proper
cable connections:
6.2.1 Verify that the BioFlo 2000 Fermentor ON / OFF ROCKER SWITCH is
set to OFF.
6.2.2 Connect the flat female end of the ANTI–FOAM MODULE
INTERCONNECTING CABLE to the 15 PIN POWER / CONTROL
CONNECTOR on the back panel of Anti–Foam Module.
6.2.3 Tighten the thumbscrews on the ANTI–FOAM MODULE
INTERCONNECTING CABLE until secure.
6.2.4 Connect the pinned male end of the ANTI–FOAM MODULE
INTERCONNECTING CABLE to the flat AF 2000 CONNECTOR on the
back panel of the BioFlo 2000 Fermentor.
BioFlo 2000 Fermentor
Back Panel AF 2000
Connector
HeaterCooling
Valve RTD
Recorder
XX 2000
A
F 2000
FP 2000
p
H 2000
DO
RS 422/232
120
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7. PREPARATION BEFORE THE AUTOCLAVE
7.1 VESSEL HEADPLATE ASSEMBLY
Perform the following steps to properly insert the CONDUCTANCE PROBE
FITTING into the Port on the Vessel HeadPlate.
7.1.1 Screw the CONDUCTANCE PROBE FITTING (supplied) onto the Vessel
HeadPlate. (See HeadPlate Illustrations)
7.1.2 Verify that you screwed the CONDUCTANCE PROBE FITTING into the
indicated 6 mm Port on the Vessel HeadPlate.
Conductance Probe Fitting
Detail
Back Ferrule
Front Ferrule
Knurl Nut
7.1.3 Verify that the FRONT and BACK FERRULES are properly inserted in
the CONDUCTANCE PROBE FITTING.
7.1.4 Replace one of the VESSEL MOUNTING ARM screws with a GROUND
POST SCREW.
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2 Liter Vessel HeadPlate
pH Electrode
Cooling Coil 2
Cooling Coil 1
Sparger
3 Way Inlet
Inoculation
Thermowell
DO Electrode
Spare/Exhaust Foam/Level
Spare/Exhaust Condenser
Sample/Harvest
Ground Post
Front
#NOTE:
When you use a FOAM PROBE and a LEVEL PROBE simultaneously, use the
FOAM/LEVEL PORT and SPARE/EXHAUST PORT for the Probes. Use the
EXHAUST PORT CONDENSER (M1230–9413) on the SPARE/EXHAUST
CONDENSER PORT.
AF 2000 User’s Guide