NEW BRUNSWICK SCIENTIFIC BioFlo 110 User manual

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BioFlo 110
Modular Benchtop
Fermentor
MANUAL NO: M1273-0054
Revision H
August 8, 2007
NEW BRUNSWICK SCIENTIFIC CO., INC.
BOX 4005 •44 TALMADGE ROAD •EDISON, NJ 08818-4005
Telephone: [phone] •1-800-631-5417
Fax: [phone] •Telex: 4753012 NBSCO
Internet: http://www.nbsc.com •E-mail: [email protected]

ii
BioFlo 110 Modular Fermentor M1273-0054 User’s Guide

iii
New Brunswick Scientific User’s Guide
INTERNATIONAL OFFICES:
BELGIUM
New Brunswick Scientific NV-SA
Stationsstraat 180/4
3110 Rotselaar
België/Belgique
Tel: [phone]
Fax: [phone]
E-mail: [email protected]
GERMANY
New Brunswick Scientific GmbH
In Der Au 14
D-72622 Nürtingen
Deutschland
Tel: [phone]
Fax: [phone]
E-mail: [email protected]
CHINA
New Brunswick Scientific Co., Inc.
A903, 904 Yin Hai Building
No. 250, Cao Xi Road
Shanghai 200235, P.R. China
Tel: [phone] or 5966
Fax: [phone]
E-mail: [email protected]
THE NETHERLANDS
New Brunswick Scientific BV
Kerkenbos 1101, 6546 BC Nijmegen
P.O Box 6826, 6503 GH Nijmegen
Nederland
Tel: [phone]
Fax: [phone]
E-mail: [email protected]
FRANCE
New Brunswick Scientific SARL
12-14, Rond Point des Champs Elysées
75008 Paris
France
Tel: [phone]
Fax: [phone]
E-mail: [email protected]
UNITED KINGDOM
New Brunswick Scientific (UK) Ltd.
17 Alban Park
St. Albans, Herts. AL4 0JJ
United Kingdom
Tel: [phone] or 0800 581331
Fax: [phone]
E-mail: [email protected]
Web: www.nbsuk.co.uk

iv
BioFlo 110 Modular Fermentor M1273-0054 User’s Guide
WARNING!
This product is not designed to contain gases within the range of their
lower explosion limit (LEL) and their upper explosion limit (UEL).
If your process requires or produces gases, be sure to verify their LEL
and UEL concentration range (available online).
CAUTION!
This equipment must be operated as described in this manual.
If operational guidelines are not followed, equipment damage and
personal injury can occur. Please read the entire User’s Guide before
attempting to use this unit.
Do not use this equipment in a hazardous atmosphere or with hazardous
materials for which the equipment was not designed.
New Brunswick Scientific Co., Inc. (NBS) is not responsible for any
damage to this equipment that may result from the use of an accessory
not manufactured by NBS.
WARNING!
High voltage.
Always make sure this equipment is properly grounded.

v
New Brunswick Scientific User’s Guide
Copyright Notice
New Brunswick Scientific Company, Inc.
Box 4005 44 Talmadge Road
Edison, New Jersey 08818-4005
©Copyright 2007 New Brunswick Scientific Co., Inc.
All Rights Reserved.
Reproduction, adaptation, or translation without prior written permission from
New Brunswick Scientific is prohibited.
Disclaimer Notice
New Brunswick Scientific Co., Inc. reserves the right to change
information in this document without notice. Updates to information in this
document reflect our commitment to continuing product development and
improvement.
Manual Conventions
NOTE:
Notes contain essential information that deserves
special attention.
CAUTION!
Caution messages appear before procedures which, if
caution is not observed, could result in damage to the
equipment.
WARNING!
Warning messages alert you to specific procedures or
practices which, if not followed correctly, could result in
serious personal injury.
Bold Text in boldface type emphasizes key words or
phrases.
This particular Warning message, whether found in the
manual or on the unit, means HOT SURFACE–and
therefore represents a potential danger to touch.
CRUSH WARNING!
Crush Warning messages alert you to specific
procedures or practices regarding heavy objects which,
if not followed correctly, could result in serious personal
injury .

vi
BioFlo 110 Modular Fermentor M1273-0054 User’s Guide
Every Instrument manufactured by the New Brunswick Scientific
Co., Inc. is warranted to be free from defects in material and work-
manship. This apparatus with the exception of glassware, lamps and
electrodes (where supplied), is warranted for 1 year against faulty com-
ponents and assembly and our obligation under this warranty is limited
to repairing or replacing the instrument or part thereof, which shall
with-in 1 year after date of shipment, prove to be defective after our
examination. This warranty does not extend to any NBS products
which have been subjected to misuse, neglect, accident or improper
installation or application; nor shall it extend to products which
have been repaired or altered outside the NBS factory
without prior authorization from the
New Brunswick Scientific Co., Inc.
W A R R A N T Y

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New Brunswick Scientific User’s Guide

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BioFlo 110 Modular Fermentor M1273-0054 User’s Guide

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New Brunswick Scientific User’s Guide
TABLE OF CONTENTS
1OVERVIEW........................................................................................................................................................ 1
1.1 DESIGN CONCEPT......................................................................................................................................... 1
1.2 PROCESS CONTROL ...................................................................................................................................... 1
1.3 COMPUTER-READY ...................................................................................................................................... 2
1.4 ENHANCING YOUR FERMENTOR................................................................................................................... 2
2INSPECTION & UNPACKING........................................................................................................................ 3
2.1 INSPECTION OF BOXES ................................................................................................................................. 3
2.2 PACKING LIST VERIFICATION....................................................................................................................... 3
3PREPARING THE LOCATION....................................................................................................................... 4
3.1 PHYSICAL LOCATION ................................................................................................................................... 4
3.2 ENVIRONMENT............................................................................................................................................. 4
3.3 UTILITIES ..................................................................................................................................................... 4
4INSTALLATION, ASSEMBLY & SET-UP..................................................................................................... 5
4.1 INTRODUCTION TO CONTROL MODULE REAR PANELS ................................................................................. 5
4.1.1 Primary Control Unit (PCU).................................................................................................................. 5
4.1.2 Power Controller.................................................................................................................................... 6
4.1.3 dO2/pH Controller ................................................................................................................................. 7
4.1.4 Level Controller...................................................................................................................................... 8
4.1.5 Gas Mix Controller................................................................................................................................. 8
4.1.6 Pump Module.......................................................................................................................................... 9
4.1.7 Other Optional Modules....................................................................................................................... 10
4.2 STACKING MODULES ................................................................................................................................. 10
4.3 INTERCONNECTING THE MODULES............................................................................................................. 12
4.4 **IMPORTANT WARNINGS** ..................................................................................................................... 14
4.5 VESSEL ASSEMBLY: NON-JACKETED........................................................................................................ 16
4.5.1 Headplate ............................................................................................................................................. 18
4.5.2 Install Heat Blanket.............................................................................................................................. 20
4.5.3 Install Vessel in Vessel Stand ............................................................................................................... 21
4.5.4 Install Baffle (14.0L Fermentation Vessels ONLY) .............................................................................. 21
4.5.5 Install Impeller(s)................................................................................................................................. 22
4.5.6 Install Cooling Coil.............................................................................................................................. 23
4.5.7 Install Sparger (3.0L, 7.5L & 14.0L Vessels)....................................................................................... 23
4.5.8 Install Harvest Tube ............................................................................................................................. 24
4.5.9 Install Sampler Tube............................................................................................................................. 24
4.5.10 Install Thermowell........................................................................................................................... 24
4.5.11 Install Foam Probe.......................................................................................................................... 24
4.5.12 Install Foam Exhaust Tube.............................................................................................................. 25
4.5.13 Install Level Probe(s)....................................................................................................................... 25
4.5.14 Install Addition Tube(s) ................................................................................................................... 25
4.5.15 Install pH Probe............................................................................................................................... 25
4.5.16 Install dO2 Probe............................................................................................................................. 27
4.5.17 Install Exhaust Condenser............................................................................................................... 28
4.5.18 Install Sampler................................................................................................................................. 30
4.5.19 Install Foam Trap............................................................................................................................ 32
4.5.20 Plug Unused Ports........................................................................................................................... 33
4.5.21 Install 1.3L, 3.0L or 7.5L Fermentation Vessel Baffle..................................................................... 34
4.5.22 Install Headplate.............................................................................................................................. 34
4.6 VESSEL ASSEMBLY: WATER-JACKETED ................................................................................................... 35
4.6.1 Headplate ............................................................................................................................................. 37

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BioFlo 110 Modular Fermentor M1273-0054 User’s Guide
4.6.2 Install Headplate Clamping Ring......................................................................................................... 40
4.6.3 Install Vessel on Base Plate.................................................................................................................. 40
4.6.4 Install Baffle (14.0L Fermentation Vessels ONLY) .............................................................................. 41
4.6.5 Install Impeller(s)................................................................................................................................. 41
4.6.6 Install Sparger...................................................................................................................................... 42
4.6.7 Install Harvest Tube ............................................................................................................................. 43
4.6.8 Install Sampler Tube............................................................................................................................. 43
4.6.9 Install Thermowell................................................................................................................................ 43
4.6.10 Install Foam Probe.......................................................................................................................... 43
4.6.11 Install Foam Exhaust Tube.............................................................................................................. 44
4.6.12 Install Level Probe(s)....................................................................................................................... 44
4.6.13 Install Addition Tube(s) ................................................................................................................... 44
4.6.14 Install pH Probe............................................................................................................................... 44
4.6.15 Install Dissolved Oxygen Probe....................................................................................................... 46
4.6.16 Install Exhaust Condenser............................................................................................................... 47
4.6.17 Install Sampler................................................................................................................................. 50
4.6.18 Install Foam Trap............................................................................................................................ 52
4.6.19 Install Antifoam System.................................................................................................................... 53
4.6.20 Plug Unused Ports........................................................................................................................... 53
4.6.21 Install 1.3L, 3.0L or 7.5L Fermentation Vessel Baffle..................................................................... 53
4.6.22 Install Headplate.............................................................................................................................. 54
4.7 INSTALL THE AGITATION MOTOR............................................................................................................... 54
4.8 INSTALL THE ROTAMETER.......................................................................................................................... 55
4.9 INSTALL LIQUID ADDITION SYSTEMS......................................................................................................... 56
4.10 MAKING CONNECTIONS ............................................................................................................................. 57
4.10.1 Power............................................................................................................................................... 57
4.10.2 Water................................................................................................................................................ 57
4.10.3 Probes and Sensors.......................................................................................................................... 58
4.10.4 Pumps .............................................................................................................................................. 61
4.10.5 Air and Optional Gases ................................................................................................................... 62
4.11 INSTALL CABLE GUIDES............................................................................................................................. 63
4.12 VOLTAGE SELECTION AND FUSING ............................................................................................................ 64
4.13 CALIBRATING THE PH PROBE..................................................................................................................... 65
5BEFORE FIRST USE....................................................................................................................................... 69
5.1 SELECT PCU DISPLAY LANGUAGE ............................................................................................................ 69
5.2 ASSIGN VESSEL ADDRESS.......................................................................................................................... 69
5.3 FOR PC SUPERVISION ................................................................................................................................70
5.3.1 Connect to Supervisory System............................................................................................................. 70
5.3.2 Select Communications Protocol.......................................................................................................... 71
5.3.3 Set Base Addresses ............................................................................................................................... 72
5.3.4 Set Chart Recorder for Data Logging .................................................................................................. 72
6STERILIZATION & REINSTALLATION................................................................................................... 75
6.1 INITIAL PREPARATION FOR AUTOCLAVING ................................................................................................ 76
6.1.1 Filling the Water Jacket........................................................................................................................ 76
6.2 ADDITIONAL PREPARATION FOR AUTOCLAVING........................................................................................ 76
6.3 AUTOCLAVING THE VESSEL ....................................................................................................................... 77
6.3.1 Sterilization Time and Temperature..................................................................................................... 78
6.4 REINSTALLING THE SYSTEM....................................................................................................................... 79
6.5 CONFIRMING PH CALIBRATION.................................................................................................................. 80
6.6 POLARIZING & CALIBRATING THE DO2 PROBE.......................................................................................... 80
6.7 POWERING THE JACKET WATER HEATER................................................................................................... 82
7OPERATION.................................................................................................................................................... 84
7.1 PRIMARY CONTROL UNIT DISPLAY & CONTROLS...................................................................................... 84

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New Brunswick Scientific User’s Guide
7.2 SELECTING THE APPLICATION.................................................................................................................... 86
7.3 MAIN SCREEN ............................................................................................................................................ 87
7.4 SET THE TEMPERATURE LOOP.................................................................................................................... 89
7.4.1 Control Mode........................................................................................................................................ 89
7.4.2 Setpoint................................................................................................................................................. 91
7.4.3 Graphic Display ................................................................................................................................... 92
7.5 SETTING THE AGITATION LOOP.................................................................................................................. 93
7.6 SETTING THE PH LOOP............................................................................................................................... 93
7.6.1 Setting a Deadband for pH control....................................................................................................... 94
7.6.2 Setting the Output Multiplier for pH Control....................................................................................... 95
7.6.3 Setting P & I Gains for pH Control...................................................................................................... 97
7.7 SETTING THE DO2 LOOP ............................................................................................................................ 98
7.8 SETTING DO2 CONTROL CASCADES........................................................................................................... 98
7.8.1 Cascades for Fermentation without Gas Mix Controller ..................................................................... 98
7.8.2 Cascades for Fermentation with Gas Mix Controller ........................................................................ 100
7.8.3 Cascades for Cell Culture without Gas Mix Controller ..................................................................... 102
7.8.4 Cascades for Cell Culture with Gas Mix Controller .......................................................................... 102
7.9 SETTING P & I GAINS FOR DO2 CONTROL............................................................................................... 105
7.10 SETTING THE GAS MIX FOR FERMENTATION............................................................................................ 106
7.11 SETTING THE GAS MIX FOR CELL CULTURE............................................................................................. 106
7.11.1 4-Gas Mode.................................................................................................................................... 107
7.11.2 Demand Mode................................................................................................................................ 107
7.11.3 100% Feed Mode........................................................................................................................... 107
7.12 SETTING THE PUMPS ................................................................................................................................108
7.12.1 Pump Operation............................................................................................................................. 108
7.12.2 Pump Setpoint and Multiplier........................................................................................................ 108
7.12.3 Control Mode................................................................................................................................. 109
7.12.4 Setting Pumps ................................................................................................................................ 109
7.13 SETTING THE PUMP CONTROL MODE: ANTIFOAM, WET ON & WET OFF ............................................... 110
7.14 INOCULATION........................................................................................................................................... 112
7.15 STERILE SAMPLING .................................................................................................................................. 112
7.16 AUTOMATIC FOAM CONTROL .................................................................................................................. 112
7.17 HARVESTING............................................................................................................................................ 113
8MAINTENANCE............................................................................................................................................ 114
8.1 CLEANING THE MODULES ........................................................................................................................ 114
8.2 PH PROBE MAINTENANCE AND STORAGE ................................................................................................ 114
8.3 DO2 PROBE MAINTENANCE AND STORAGE.............................................................................................. 114
8.4 CLEANING THE VESSELS .......................................................................................................................... 115
8.4.1 14.0L Vessel Disassembly................................................................................................................... 115
8.4.2 Vessel Cleaning Methods.................................................................................................................... 115
8.5 PORT & ADAPTER O-RING REPLACEMENT .............................................................................................. 116
8.6 HEADPLATE O-RING REPLACEMENT........................................................................................................ 117
8.7 BEARING HOUSING CAP REPLACEMENT................................................................................................... 117
8.8 REPLACING PCU FUSES........................................................................................................................... 117
9SERVICE......................................................................................................................................................... 118
9.1 TROUBLESHOOTING ................................................................................................................................. 118
10 REPLACEMENT PARTS & ACCESSORIES............................................................................................ 119
10.1 REPLACEMENT PARTS.............................................................................................................................. 119
10.2 REPLACEMENT VESSELS .......................................................................................................................... 122
10.3 MISCELLANEOUS PARTS & OPTIONAL ACCESSORIES............................................................................... 122
10.4 MODULE KITS.......................................................................................................................................... 123
11 APPENDIX A: SPECIFICATIONS............................................................................................................. 124

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BioFlo 110 Modular Fermentor M1273-0054 User’s Guide
11.1 MODULES................................................................................................................................................. 124
11.2 PUMP FLOW RATES .................................................................................................................................. 126
11.3 TUBING .................................................................................................................................................... 127
11.4 VESSELS................................................................................................................................................... 127
11.4.1 Non-Jacketed Vessel Dimensions for Autoclaving (Vertical) ........................................................ 127
11.4.2 Non-Jacketed Vessel Dimensions for Autoclaving (Inclined)........................................................ 128
11.4.3 Water-Jacketed Vessel Dimensions for Autoclaving (Vertical) ..................................................... 129
11.4.4 Non-Jacketed Vessel Dimensions for Autoclaving (Inclined)........................................................ 129
12 APPENDIX B: TECHNICAL INTERVENTIONS..................................................................................... 131
12.1 REPLACING POWER CONTROLLER FUSES................................................................................................. 131
12.2 REPLACING PCU BATTERIES ................................................................................................................... 132
12.3 VOLTAGE SELECTION............................................................................................................................... 133
12.4 CHANGING PCU FUSING.......................................................................................................................... 134
12.4.1 Converting from Single Fuse to Double Fuse................................................................................ 134
12.5 P&I CONTROL EQUATIONS ...................................................................................................................... 136
12.5.1 pH .................................................................................................................................................. 136
12.5.2 dO2 ................................................................................................................................................ 137
13 APPENDIX C: COMMUNICATIONS PROTOCOLS.............................................................................. 138
13.1 COMMUNICATIONS SETTINGS................................................................................................................... 138
13.1.1 AFS/ModBus Pin Connections....................................................................................................... 139
13.2 AFS (BIOCOMMAND) COMMUNICATIONS PROTOCOL ............................................................................. 139
13.2.1 General Specifications................................................................................................................... 139
13.2.2 Protocol ......................................................................................................................................... 140
13.2.3 Requests & Response Headers....................................................................................................... 140
13.2.4 Change Commands........................................................................................................................ 141
13.2.5 Message Formats........................................................................................................................... 141
13.3 MODBUS COMMUNICATIONS PROTOCOL ................................................................................................. 141
13.3.1 Register Map.................................................................................................................................. 142
14 APPENDIX D: THERMAL MASS FLOW CONTROLLER.................................................................... 143
14.1 INSTALLATION ......................................................................................................................................... 143
14.2 LOADING SOFTWARE UPDATE.................................................................................................................. 145
14.3 OPERATION .............................................................................................................................................. 146
14.3.1 Fermentation Mode Setup.............................................................................................................. 147
14.3.2 Fermentation Mode Cascades ....................................................................................................... 149
14.3.3 Cell Culture Mode Setup................................................................................................................ 150
14.3.4 Cell Culture Mode Cascades ......................................................................................................... 151
14.4 CLEANING................................................................................................................................................ 152
14.5 SPECIFICATIONS ....................................................................................................................................... 153
14.6 TROUBLESHOOTING ................................................................................................................................. 153
15 APPENDIX E: DRAWINGS......................................................................................................................... 154
15.1 MODULE SCHEMATICS ............................................................................................................................. 154
15.2 LIST OF DRAWINGS .................................................................................................................................. 162
16 APPENDIX F: PORTS & ADAPTERS....................................................................................................... 164
17 INDEX ............................................................................................................................................................. 165

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New Brunswick Scientific User’s Guide
1
1 O OV
VE ER RV VI IE EW W
1.1 Design Concept
The BioFlo 110 is a modular fermentation system for microbial and cell culture
applications. It features a modern, easy-to-use control interface, plus an available family
of vessels, controller modules, and other accessories that address the diverse needs of the
bioprocessing community.
A complete range of water-jacketed and non-jacketed fermentation and cell culture vessels
is available to cover 0.4 through 14.0 liters. For temperature control, non-jacketed vessels
use an external heating blanket and an internal cooling coil. Jacketed vessels use an
external jacket water temperature controller.
Modularity means you can position components to suit your workspace and working style.
The controller modules can be vertically stacked to conserve bench space. Modularity
also makes it easy and economical to adapt your system for different tasks at any time.
As an autoclavable fermentor, the BioFlo 110 requires an external sterilizing apparatus
that is large enough to accept the complete vessel assembly.
1.2 Process Control
The heart of the BioFlo 110 is the Primary Control Unit (PCU). The PCU serves as the
operator interface for one to four vessels. Its bright graphic display and clearly marked
keypad serve as the control center for all attached vessels and their associated loop
controllers. Four-channel output to a strip-chart recorder is available from the PCU.
NOTE:
No more than 16 modules (any combination) can be supported by one PCU.
The BioFlo 110 Power Controller is required for temperature and agitation control. It also
provides power for liquid pumps and other control modules. The rotameter and cooling
water valve are mounted on the outside of the Power Controller.
Additional control modules and sensors enable expanding a basic temperature/agitation/
airflow fermentor to include control of pH, dissolved oxygen (dO2), antifoam addition,
removal and addition of nutrients and other liquids, and blending inlet air with up to three
other gases.

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BioFlo 110 Modular Fermentor M1273-0054 User’s Guide
1.3 Computer-Ready
The BioFlo 110 is compatible with both ModBus and New Brunswick Scientific’s
BioCommand communications protocols. Optional BioCommand software adds automatic
data logging, centralized monitoring and control, and process programming capabilities to
one or more fermentors.
1.4 Enhancing Your Fermentor
Other accessories are available and new ones will be added to extend the capabilities of
the BioFlo 110. Whenever your bioprocessing needs or applications change, contact your
New Brunswick Scientific sales representative for a current description of BioFlo 110
products.

3
New Brunswick Scientific User’s Guide
2
2 I IN
NS SP PE EC CT TI IO ON N &
& U UN
NP PA AC CK KI IN NG G
2.1 Inspection of Boxes
After you have received your order from New Brunswick Scientific, inspect the boxes
carefully for any damage that may have occurred during shipping. Report any damage
to the carrier and to your local NBS Sales Order Department immediately.
2.2 Packing List Verification
Unpack your order, saving the packing materials for possible future use. Also be sure
to save the User’s Guide, for instruction and reference.
Verify against your NBS packing list that you have received the correct materials, and
that nothing is missing. The part numbers on the packing list correspond to part
numbers and/or manufacturing numbers. You will find the number affixed to each
item, either on a paper sticker on the packaging or on the serial number identification
sticker on the equipment itself.
If any part of your order was damaged during shipping or is missing, or fails to
operate, please fill out Customer Satisfaction Form 6300 (packed in the envelope with
your warranty card) and return it by fax or mail. You can also call the New Brunswick
Scientific Service Department.

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BioFlo 110 Modular Fermentor M1273-0054 User’s Guide
3
3 P PR
RE EP PA AR RI IN NG G T TH HE E L
LO
OC CA AT TI IO ON N
3.1 Physical Location
The surface where you place the BioFlo 110 should be smooth, level and sturdy.
Ensure that there is enough space around the back and the front of the combined
system for proper operation and access.
3.2 Environment
The BioFlo 110 operates best under the following conditions:
• Ambient temperature range of 10°C to 35° C
• Relative humidity up to 80% non-condensing
3.3 Utilities
Utility Requirements (minimum-maximum)
Water (50 μm filtration)
5-10 PSIG (0.34-0.68 kg/cm2)
Air 3-10 PSIG (0.20-0.68 kg/cm2)
O2 3-10 PSIG (0.20-0.68 kg/cm2)
CO2 3-10 PSIG (0.20-0.68 kg/cm2)
Gases (optional)
N2 3-10 PSIG (0.20-0.68 kg/cm2)
100V 90-100 VAC 50/60 Hz 10 Amp
120V 108-132 VAC 50/60 Hz 10 Amp
220V 198-242 VAC 50/60 Hz 6 Amp
Electricity
240V 216-264 VAC 50/60 Hz 6 Amp
NOTE:
All gases supplied should be medical grade.

5
New Brunswick Scientific User’s Guide
4
4 I IN
NS ST TA AL LL LA AT TI IO ON N,
, A AS
SS SE EM MB BL LY Y &
& S SE
ET T-
-U UP
P
4.1 Introduction to Control Module Rear Panels
4.1.1 Primary Control Unit (PCU)
Figure 1: Primary Control Unit (PCU) Rear Panel
Refer to Figure 1 above to visually locate the following features on the rear panel:
• FLOPPY DRIVE An internal A: drive for field upgrades to the interface
software.
• POWER SWITCH On/Off rocker switch.
• AC CONNECTION Inlet for power cord (supplied), either for connection to
Power Controller outlet or for electrical service outlet.
• FUSE HOLDER Easy access to replace 250V 2-amp Slo-Blo®fuse.
Flopp
y
Drive
Power Switch
I = On
O = Of
f
Power Inlet
25-pin
Connecto
r
RS-485
Connectors
(daisy chain to
next module)
9-pin Recorder
Connector
PCU serial number
Fuse
Holde
r

6
BioFlo 110 Modular Fermentor M1273-0054 User’s Guide
• AFS/MODBUS
CONNECTOR 25-pin D connector for any supervisory software you
may add.
• CONTROLLER BUS
CONNECTORS Two RS-485 9-pin D connectors, one above the other.
Use one to connect the Power Controller to the PCU with
the cable supplied.
• RECORDER
CONNECTOR One 9-pin D connector, reserved for use with any analog
recorder you may add. 2.5V output supplies data
records.
4.1.2 Power Controller
Figure 2: Power Controller Rear Panel
FUSE
DRAWER
POWER INLET
(Connect to Mains
with supplied
power cord)
PERISTALTIC PUMP CONNECTIONS
(3 assignable by operator)
POWER OUTLET
(Connect to PCU
with supplied
power cord)
CONNECTOR
for WATER
SOLENOID
VALVE CABLE
INDICATOR
LIGHTS
4-PIN UNIT ADDRESSS
SELECTOR
LOCATION
UNIT ADDRESS
SELECTION
REFERENCE
TABLE
RS-485 CONNECTORS
(daisy-chain to other modules;
cap unused connecto
r
with Terminator plug)
RS-485 CABLE
(provided)

7
New Brunswick Scientific User’s Guide
Refer to Figure 2 above to visually locate the following features:
• WATER SOLENOID
VALVE Located on the side panel (not visible in drawing).
Provides water from outside source for cool temperature
control within the vessel and to the optional exhaust
condenser.
• WATER SOLENOID
CONNECTOR Located on the rear panel. Control connection for the
water solenoid valve.
• VOLTAGE SELECTOR Located in the fuse drawer in the rear panel, next to the
power cord connection.
• AC CONNECTION Located on the rear panel. Input connection, using the
power cord supplied, to the electrical service.
• PCU CONNECTION Located on the rear panel. Output connection, using the
power cord supplied, to power the PCU.
• CONTROLLER BUS
CONNECTORS Located on the rear panel. Two RS-485 9-pin D
connectors, one above the other. Use one to connect the
Power Controller to the PCU with the cable supplied.
• UNIT ADDRESS
SELECTOR Located on the rear panel. Four two-position (on/off)
switches used to identify the vessel address, uniquely
associating this module to one particular vessel.
4.1.3 dO2/pH Controller
Figure 3: dO2/pH Controller – Rear Panel
Module Serial
Numbe
r
RS-485 Connectors
(daisy-chain to othe
r
modules)
Indicato
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