NUVE ST 30 User manual

NÜVE SANAYİ MALZEMELERİ İMALAT VE TİCARET A.Ş
ST 30
SHAKING WATER BATH
USER’S MANUAL
Z14.K 25 282 Rev.No: 03 Rev. Date: 10 / 2017

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Dear Nüve User,
We would like to take this opportunity to thank you for preferring this Nüve product. Please
read the operating instructions carefully and keep them handy for future reference.
Please detain the packing material until you see that the unit is in good condition and it is
operating properly. If an external or internal damage is observed, contact the transportation
company immediately and report the damage. According to ICC regulations, this
responsibility belongs to the customer.
While you are operating the instrument please;
obey all the warning labels,
do not remove the warning labels,
do not operate damaged instrument,
do not operate the instrument with a damaged cable,
do not move the instrument during operation.
In case of a problem contact your Nüve agent for an authorized service or maintenance.
The validity of the guarantee is subject to compliance with the instructions and precautions
described in this manual.
Nüve reserves the right to improve or change the design of its products without any
obligation to modify previously manufactured products.
Information contained in this document is the property of Nüve. It may not be duplicated or
distributed without its permission.
SANAYİ MALZEMELERİ
İMALAT VE TİCARET A.Ş.
Saracalar Mah. Saracalar Kümeevleri No: 4/2
Akyurt 06750 Ankara-TURKEY
Tel : [phone]-32
Fax : [phone]
Sales : [e-mail]
Technical Service: [e-mail]

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WARRANTY CERTIFICATE
1. Nüve warrants that the equipment delivered is free from defects in material and
workmanship. This warranty is given for a period of two years. The warranty period begins
from the delivery date.
2. Warranty does not apply to parts normally consumed during operation or general
maintenance or any adjustments described in the operating instructions provided with the
instrument.
3. Nüve does not accept any liability in case where the goods are not used in accordance
with their proper intent.
4.The warranty may not be claimed for damages incurred during the shipment, for damages
resulting from improper handling or use, abuse, fire, liquid spillage, tampering or
unauthorized repairs by any persons, use of defective or incompatible accessories,
exposure to abnormally corrosive conditions, use of the product in non-standard
environmental conditions, including but not limited to failure to meet requirements of
ambient temperature, lubrication, humidity or magnetic field influences, from the defects in
maintenance, negligence, bad functioning of auxiliary equipment, in the case of force
majeure or accident and incorrect power supply.
5. In the event of failure, Nüve shall be under no liability for any injury, or any loss or damage
as the result of the failure other than the guarantee conditions.
BEFORE OPERATING THE INSTRUMENT THIS MANUAL SHOULD BE READ
CAREFULLY.
INFORMATION CONTAINED IN THIS DOCUMENT IS THE PROPERTY OF NÜVE. IT MAY
NOT BE DUPLICATED OR DISTRIBUTED WITHOUT PERMISSION.
THE VALIDITY OF THE GUARANTEE IS SUBJECT TO THE OBSERVATION OF THE
INSTRUCTIONS AND PRECAUTIONS DESCRIBED IN THIS MANUAL.
Lifetime of the device is 10 years.
PLEASE REGISTER ONLINE TO VALIDATE WARRANTY:
To register your warranty online, please visit our web page www.nuve.com.tr and fill in
Warranty Registration Form.

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TABLE OF CONTENTS
SECTION 1...............................................................................................................................................5
INTRODUCTION......................................................................................................................................5
1.1 USE AND FUNCTION................................................................................................................5
SECTION 2...............................................................................................................................................6
TECHNICAL SPECIFICATIONS..............................................................................................................6
2.1 TECHNICAL SPECIFICATIONS TABLE....................................................................................6
2.2 OPTIONAL ACCESSORIES......................................................................................................6
SECTION 3...............................................................................................................................................7
PRECAUTIONS AND LIMITATIONS OF USAGE...................................................................................7
SECTION 4...............................................................................................................................................7
SYMBOLS................................................................................................................................................7
SECTION 5...............................................................................................................................................8
INSTALLATION .......................................................................................................................................8
5.1 ENVIRONMENTAL CONDITIONS.............................................................................................8
5.2 HANDLING AND TRANSPORTATION......................................................................................8
5.3 UNPACKING..............................................................................................................................8
5.4 MAINS SUPPLY.........................................................................................................................8
5.5 POSITIONING............................................................................................................................8
5.6 GENERAL PRESENTATION.....................................................................................................9
5.7 CONTROL PANEL.....................................................................................................................9
5.7.1 Explanations and Functions for Display and Control Panel ............................................ 10
5.8 PRIOR TO OPERATION......................................................................................................... 11
5.8.1 Filling Liquid..................................................................................................................... 11
SECTION 6............................................................................................................................................ 12
OPERATING PRINCIPLES................................................................................................................... 12
6.1 OPERATING THE UNIT.......................................................................................................... 12
6.2 PROGRAMMING .................................................................................................................... 12
6.2.1 PREPARATION OF USER SETTINGS........................................................................... 12
6.2.2 oP Operator Menu Parameters........................................................................................ 13
6.3 PROGRAMMING SUMMARY................................................................................................. 14
6.4 COMPLETION OF THE OPERATION .................................................................................... 16
SECTION 7............................................................................................................................................ 17
PERIODICAL MAINTENANCE AND CLEANING................................................................................ 17
7.1 PERIODICAL MAINTENANCE ............................................................................................... 17
7.2 CLEANING.............................................................................................................................. 17
SECTION 8............................................................................................................................................ 17
DISPOSAL MANAGEMENT CONCEPT .............................................................................................. 17
SECTION 9............................................................................................................................................ 17
TROUBLESHOOTING.......................................................................................................................... 17
9.1 ERROR CODES AND EXPLANATIONS................................................................................. 18
9.2 FUSE REPLACEMENT........................................................................................................... 18
SECTION 10.......................................................................................................................................... 19
ELECTRICAL CIRCUIT DIAGRAM...................................................................................................... 19
SECTION 11.......................................................................................................................................... 20
WARNING LABEL................................................................................................................................ 20

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SECTION 1
INTRODUCTION
1.1 USE AND FUNCTION
ST 30 shaking water bath is designed to be used in microbiology, biotechnology, research
and industrial laboratories mainly tissue culture, bacterial incubation, enzyme reactions,
fermentation and other general and special applications.
ST 30 maintains incubation and heating temperatures between 5 ⁰C above the ambient
temperature and 99.9 ⁰C and keeps the temperature stable within the given tolerances.
Besides heating, ST 30 has a shaking function. The shaking speed can be set between 20
rpm and 250 rpm.
ST 30 has a microprocessor control system. The heating is based on PID control system.
There are digital display both for heating and shaking functions. Each function for
temperature and shaking has timer which can be programmed up to 99.9 hours including
hold position.
Microprocessor control system will shut down the temperature sensor and in case of
malfunctions that may occur in the control system, alarm system will be activated and the
user is warned visually and audibly. The study data are recorded in the memory and can be
transferred to external USB memory. At the same time, unauthorized persons have been
blocked permission to change parameters with improved password menu.
The tank of ST 30 is made of stainless steel and all the materials in contact with liquid are
made of stainless material. The tank is insulated with glass wool for operating homogenous
temperature distribution.
The ST 30 water bath is manufactured according to following standards;
EN 61010-1, EN 61010-2-051, EN 50419, EN 61326.
This device is in compliance with WEEE Regulation.

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SECTION 2
TECHNICAL SPECIFICATIONS
2.1 TECHNICAL SPECIFICATIONS TABLE
Technical Specifications
ST 30
Temperature range
Ambient temperature +5ºC / 99.9ºC
Temperature sensor
Fe –Const.
Control System
N-PrimeTM
Temperature set and display sensitivity
0.1ºC
Temperature variation (37 ºC)
< ± 0.3 ºC
Temperature fluctuation (37 ° C)
±0.1 ºC
Shaking speed
20 - 250 rpm
Shaking speed adjustment sensitivity
1 rpm
Timer
1 minute –99.9 hours + hold position
Delayed Start Timer
1 minute –99.9 hours
Memory
3000 data*
Power supply
230 V, 50 Hz.
Power consumption
1550 Watt
Tank volume
30 lt
Internal material
Stainless steel
External material
Epoxy-polyester powder coated stainless steel
Internal dimensions (WxDxH) mm
300 x 505 x 190
Overall dimensions (WxDxH) mm
303 x 626 x 406
*Shows the number of lines for each work done. Temperature, time and if there is
error code consist of 1 line.
2.2 OPTIONAL ACCESSORIES
K 04 197 plexiglass lid (Temperature resistance 60 ºC)
K 52 004 stainless steel lid
TUBE RACK AND SHELVES
A 08 051 Tube rack 52xØ13 mm
A 08 050 Tube rack 30xØ16 mm
A 08 021 Tube rack 27xØ18 mm
A 08 049 Tube rack 12xØ30 mm
R 01 015 6x250 ml shelf
R 01 078 12x100 ml shelf
R 01 038 4x500 ml shelf
R 01 079 2x1000 ml shelf
R 01 036 6x100 ml shelf

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SECTION 3
PRECAUTIONS AND LIMITATIONS OF USAGE
Do not operate the instrument for purposes other than its main purpose.
The instrument should only be used by authorized and trained staff after the instruction
manual has been read carefully. Only authorized technical staff can handle the product in
case of a failure.
Only original spare parts and original accessories supplied by Nüve should be used.
Correctly grounded power supply should be used.
Check the following carefully,
Liquids are not heated in sealed containers;
The samples which may liquefy and expand are not in a closed container.
The set temperature is not higher than the boiling points of the samples;
The sizes of the containers of the liquids which may expand during heating are so big
that they do not overflow;
The set temperature does not destroy the structure of the samples;
The vapor and gases generated during the operation are not harmful to human health or
flammable or explosive.
SECTION 4
SYMBOLS
Symbol in the operating instructions:
Attention, general hazard area.
This symbol refers to safety relevant warnings and indicates possibly dangerous
situations.
The non-adherence to these warnings can lead to material damage and injury to
personal.

Symbol in the operating instructions:
This symbol refers to important circumstances.

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SECTION 5
INSTALLATION
5.1 ENVIRONMENTAL CONDITIONS
The water bath is designed to operate safely under the following conditions:
•Indoor use only
•Ambient temperature: 5°C to 40°C.
•Maximum relative humidity for temperature up to 22°C: 80%.
•Maximum altitude: 2000 m.
•Temperature for maximum performance: 15°C / 25°C.
5.2 HANDLING AND TRANSPORTATION
All handling and transportation must be carried out by using proper equipment and
experienced staff. The instrument must be supported underneath and never be turned upside
down.
5.3 UNPACKING
Open the packing cardboard box. Remove the nylon packing wrapped around the device.
The items provided with the device are listed below, please check them.
1 ea. user’s manual
1 ea. warranty certificate
1 ea. power cable
5.4 MAINS SUPPLY
The water bath requires 230 V, 50/60 Hz.
Please make sure that the supplied mains matches the required power ratings which are
written on the name plate of the instrument located at the back of the steam sterilizer.
Always plug-in the instrument to correctly grounded sockets.
A supply fitted with a circuit breaker should be used for protection against indirect
contact in case of an isolation fault.
5.5 POSITIONING
Check that no damage occurred during transportation.
Check that the positioning is suitable for the users.
Support the water bath underneath and carry it to its place carefully.
Make sure that the bench that the water bath is positioned is resistant to the weight of the
instrument and vibration free.

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Check that the water bath is stable on its four pads.
Check that the positioning of the water bath prevents interference with other equipment in
the near surrounding.
5.6 GENERAL PRESENTATION
Figure 1
5.7 CONTROL PANEL
Figure 2
Display and
Control Panel
Shaking tank
Input USB

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5.7.1 Explanations and Functions for Display and Control Panel
01-Temperature Display
This display shows
Sterilization chamber temperature during “stand-by” and during the operation (thermometer
position),
During program preparation, the temperature setting values or the alarm setting values,
Failure codes,
EoF warning when power is interrupted,
The set temperature values and alarm values.
02-Time Display
This display shows the values set for time during program preparation. Also; this display
shows he time values in the control of the settings.
03-Encoder Button
The encoder button has two physical movements. The button turn clockwise and
anticlockwise to increase or decrease the temperature and time values of the program. Also,
the button press for select / confirm.
04-Start / Stop Button
Used button to start the device to operating at set values or to stop the operation.
05-Heating Lamp
The led is “on” during heating, it indicates that the heating process is carried out.
06-“Operating The Program” Lamp
A lamp indicating that the program is running as soon as the device is started.
07-Data Transfer Lamp
The lamp indicates that the records are transferred in the memory or the file is transferred
during software update.
08-Alarm Led
This led flashes when there is a warning or error on the device.
01- Temperature display
09- “End of the program” Lamp
02- Time display
10- Usb Lamp
03- Encoder Button
11- Back / Alarm Mute Button
04- Start / Stop Button
12- Shaking speed display
05- Heating Lamp
13-Shaking process start / stop button
06- “Operating the program” Lamp
14- Shaking speed value increase button
07- Data Transfer Lamp
15- Shaking speed value decrease button
08-Alarm Led

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09-“End of The Program” Lamp
It is a warning lamp that indicates that the running program is finished.
10-Usb Lamp
This lamp is on when connected to a USB external memory.

The device supports up to 8 GB of external memory.
11-Back / Alarm Mute Button
This button is used to silence audible alarms in case of error and cancel the changes in the
menu.
12-Shaking Speed Display
Shaking speed unit is displayed as RPM.
13-Shaking Process Start/Stop Key
The key is used to start the set program and stop the running program.
14-Shaking Speed Value Increase Key
The keys are used to increase the shaking speed value on the display while adjusting the
shaking speed.
15-Shaking Speed Value Decrease Key
The keys are used to decrease the shaking speed value on the display while adjusting the
shaking speed.
5.8 PRIOR TO OPERATION
5.8.1 Filling Liquid
Fill the tank with distilled water or liquid and put the samples up to the maximum line
MAX (see Figure 3).
Do not run the device unless distilled water or liquid is fılled up to max line.
The distilled water or liquid level above the max level may cause unexpected
problems.
Make sure that the filled liquid is not flammable or explosive at the operation
temperature.

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Figure 3
SECTION 6
OPERATING PRINCIPLES
6.1 OPERATING THE UNIT
Switch on ST 30 shaking water bath by using the power switch and ensure that the power
switch led is on.
Observe that display and control is activated.
Learn the functions of display and control panel (see. Section 5.7.1).
Set the values and start the operation.
6.2 PROGRAMMING
6.2.1 PREPARATION OF USER SETTINGS
The device has a password protected menu. The password is set to “000” when the device
first starts. Follow the below steps to change the password, update current date / time
information and access the operator's menu where other settings are made.
Max
level

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Wait by pressing the encoder button.
Lift your hand when you see "oP" on the temperature
display and again press the encoder button. If the
device has a menu protection password, password
screen “oPS” will come on the temperature display.
You enter the set password to turn the encoder button
right and left. ( The password will not be asked if the
device is newly installed.) Confirm the password by
pressing the encoder button. Observe that the
parameter numbers on the temperature display change
with each pressing the encoder button. For operator
menu parameter descriptions (see section 6.1.1). You
can set the parameter values by turning the encoder
button right or left on time display.
Again press the encoder button and confirm the set
value. Press the back button to return the work screen.
6.2.2 oP OPERATOR MENU PARAMETERS
1: Recording Period: This time is recording period of temperature and error information.
2: Lid Alarm Time: Not used for ST models.
3: Lid Alarm Range: Not used for ST models.
4: Timer Set Band: When the read temperature reaches the "Set Temperature - TIMER SET
BAND" value, the time starts counting backwards.
5: Buzzer ON/OFF: The alarm sound on/off 0: OFF 1: ON
6: Date Setting - Year: Two digits are displayed the year information of date. If updating is
necessary, change.
7: Date Setting - Month: The month information of date is displayed. If updating is
necessary, change.
8: Date Setting - Day: The day information of date is displayed. If updating is necessary,
change.
9: Time Setting - Hour: The hour information of time is displayed. If updating is necessary,
change.
A: Time Setting - Minute: The minute information of time is displayed. If updating is
necessary, change.
B: Time Setting –Second: The second information of time is displayed. If updating is
necessary, change.
C: Date / Time Update: 0: No change 1: Update date / time according to the entered values.
The entered values are considered as current Date / Time information when 5, 6, 7, 8, 9, A
parameters are changed and B parameter is set to 1.
D: Password: The password used to enter the operator parameters. This password used
when you want to change the set values. No password if 0 is selected.

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6.3 PROGRAMMING SUMMARY
You can set the steam sterilizer on stand-by. Follow the below steps to set and save the
values.
Push the encoder button
By pushing the encoder button select SET
menu.
See that second LED flashes in the
temperature display, again push the encoder
button.
See the parameter flashing on the temperature
display.
By turning the encoder button set operating
temperature value.
Push the encoder button and save set value.
See the parameter flashing on the temperature
display.
By turning the encoder button set operating Set
alarm value. If the temperature is out of Set
alarm value, audible and visual alarm will be
activated.

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Push the encoder button and save set value.
Turn the encoder button to the right.
See that second LED flashes in the time
display, again push the encoder button.
See the parameter flashing on the time display.
By turning the encoder button set operating
time value (01 minute to 99 hours 54 minutes
or Hold).
Push the encoder button and save set value.
See 'dLY' in the temperature display.
By turning the encoder button set operating
delay time value. If 'Off' is selected, heating will
start without delay. If any numerical value is
selected; After pressing Start, it starts heating
after the set delay time (01 minute to 99 hours
54 minutes).
.
Push the encoder button and save set value.
Push ‘the start button’ and start the program.
In order to display the set values during the operation, push the encoder button
once. The values set on the temperature display and the time display of the device
will appear for 5 seconds.

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The set time start to count after the device reaches the set temperature.
6.4 COMPLETION OF THE OPERATION
Observe that the set program is completed (See “End” and “End of the program
lamp”).
Push the heating process stop key in order to stop the heating. If the device
functions with shaking, push the shaking process stop key to stop the shaking
process.
Take the samples out at the required temperature and time.
When the device is unloaded, remove the undesirable effects which are caused by
the samples.
You may leave the device at stand-by position or switch it off.
Operating records are transferred to the usb port attached a USB memory.
Check the liquid level in the tank during long operations and refill to the max line if
necessary.
The usb led and the data transfer led on the control panel turn on during transfer of data
in memory and the transfer process starts automatically. Do not remove external
memory from usb port without the data transfer led turn off and the audible alarm
finished.
Records are transferred to external memory when external memory is connected to the
Usb port. For get the records without program ending, Usb memory, hold down the
"Mute" button for 3 seconds until "Data Transfer Lamp" lights up, then remove from Usb
port.

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SECTION 7
PERIODICAL MAINTENANCE AND CLEANING
7.1 PERIODICAL MAINTENANCE
The shaking water bath does not require any periodical maintenance.
7.2 CLEANING
Prior to cleaning please unplug the device and clean the device at the room temperature.
A piece of wet cloth may be used in order to remove the dust and dirt from the device.
Mild detergent use is recommended to remove difficult dust and dirt.
Please be aware of the undesirable effects of the chemicals and be careful while applying
them.
Protect the device against rust from outside. Please clean the rust as soon as possible
when it is detected.
After all work has been completed, drain all fluid from bath reservoir.
SECTION 8
DISPOSAL MANAGEMENT CONCEPT
The user is responsible for the proper disposal of each individual component.
All parts which may comprise potentially infectious materials have to be disinfected by
suitable validated procedures (i.e. autoclaving, chemical treatment) prior to disposal.
SECTION 9
TROUBLESHOOTING
If the device fails to operate, please check the followings:
The power switch is on;
The fuse is not blown;
The plug is plugged-in properly;
The plug is not defective;
The installation of the plug is not defective;
The mains supply is present.

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9.1 ERROR CODES AND EXPLANATIONS
Err0:
Motor thermal is off. Contact to the authorized service.
Er1:
The temperature sensor endings are broken. The error code flashes on the temperature
display and an audible alarm sounds.
Er2:
An electronic failure occurs in the microprocessor. The error code flashes on the temperature
display and an audible alarm sounds.
Er3:
The temperature sensor measures a temperature higher than 147°C.The error code is shown
on the temperature display and an audible alarm sounds.
Er4:
The temperature sensor endings are connected in reverse. The error code flashes on the
temperature display and an audible alarm sounds.
EoF:
This error code appears if any probable power cut causes when the sterilization phase “EoF”
flashes and the audible alarm sounds on the temperature display.
If an error occurs, please contact to an authorized NÜVE AGENT to seek technical
help.
IN CASE OF ANY ERROR, THE PROGRAM İS STOPPED AUTOMATİCALLY AND
IMMEDIATELY.
9.2 FUSE REPLACEMENT
The fuses shall be always be replaced by the authorized personnel.

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SECTION 10
ELECTRICAL CIRCUIT DIAGRAM

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SECTION 11
WARNING LABEL
ST 30
FUSES (2 x 10A)