Optika Italy B-380 Series User manual

Model
B-383DK
B-380 Series
INSTRUCTION MANUAL
Ver. 3.1 2019

Page 2
1. Warning 3
2. Symbols and conventions 3
3. Safety Information 3
4. Intended use 3
5. Overview 4
6. Unpacking 6
7. Assembling 6
7.1 Assembling procedure 7
8. Summaryofbrighteldobservationprocedures 10
9. Summaryofdarkeldobservationprocedures 11
10. Use of the microscope 12
10.1 Light intensity adjustment 12
10.2 Coarse focus tension adjustment 12
10.3 Coarse upper limit knob 12
10.4 Stage 12
10.5 Dioptric adjustment 13
10.6 Adjusting the interpupillary distance 13
10.7 Condenser centering 13
10.7.1Centeringwithoutelddiaphragm 13
10.7.2Centeringwithelddiaphragm 14
10.8 Effectsoftheelddiaphragm 14
10.9 Aperture diaphragm 14
11. Darkeldmicroscopy 15
11.1 Principles of oil immersion microscopy 15
11.2 Principlesofdarkeldillumination 17
11.3 Highmagnicationdarkeldmicroscopy 18
11.4 Darkeldobservation 19
12. Microphotography 23
12.1 Use of C-mount camera 23
12.2 UseofReexcameras 23
13. Maintenance 24
14. Troubleshooting 25
Equipment disposal 27
Summary

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of
maintenance. It is built to high optical and mechanical standards and to withstand daily use. We remind you
that this manual contains important information on safety and maintenance, and that it must therefore be made
accessible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses
that does not comply with this manual.
2. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
3. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the
operation voltage of the equipment and that the lamp switch is in off position. Users should observe all safety
regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibility
to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure
safe operation of the unit.
4. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the
subject.

Page 4
5. Overview
EYEPIECES
PHOTO TUBE
OBSERVATION
HEAD
DIOPTER
ADJUSTMENT
RING
COARSE
UPPER
LIMIT KNOB
MAIN SWITCH /
BRIGHTNESS ADJUSTMENT KNOB
CONDENSER
STAGE
CONDENSER
HEIGHT
ADJUSTMENT KNOB
NOSEPIECE
OBJECTIVES

Page 5
COARSE FOCUS KNOB
FINE FOCUS KNOB
TENSION
ADJUSTMENT
RING
CONDENSER
LED
CENTERING
SCREWS
SLIDE HOLDER
X/Y MOVEMENT
KNOBS
CONDENSER
CENTERING
SCREWS
Overview (opposite side)
DARKFIELD
CONDENSER
SOCKET

Page 6
6. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge
of the container and lift the top half of the container. Take some care to avoid that the opti-
cal items (objectives and eyepieces) fall out and get damaged. Using both hands (one around the
arm and one around the base), lift the microscope from the container and put it on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or
other residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
7. Assembling
Once opened the B-383DK box, the microscope parts are the following:
⑪
① Frame
② Objectives
③ Observation head
④ Brighteld condenser
⑤ Darkeld condenser
⑥ Eyepieces
⑦ Allen wrench
⑧ Tension adjustment tool
⑨ Immersion oil
⑩ Power supply
⑪ Centering telescope
⑫ Dust cover
①
③
④
⑤
⑥
⑦⑧
⑩
⑨
②
⑫

Page 7
7.1 Assembling procedure
1. Insert the optical head above the stand and
tighten the screw. (Fig. 1)
• Hold the head with one hand during the
locking in order to avoid that the head falls.
2. Insert both eyepieces into the tubes of the optical
head. (Fig. 2)
3. Screw each objective into the thread of
the nosepiece, clockwise with increasing
magnication. (Fig. 3)
4. Insert the power supply jack in the connector
placed at the rear side of the microscope. (Fig. 4)
Fig. 1
Fig. 2
Fig. 3
Fig. 4

Page 8
• The microscope is delivered with two
condensers: one for brighteld and one for
darkeld. Select the suitable condenser for
the observation mode desired.
5. Lower the condenser holder using the height
condenser knob ①. (Fig. 5)
6. Insert the condenser round dovetail in the
condenser holder. (Fig. 6)
7. Tight the condenser locking screw ②. (Fig. 7)
8. (Only for darkeld condenser)
Connect the condenser jack to the connector on
the right side of the frame. (Fig. 8)
• When the condenser plug is connected, the
light coming from the microscope LED goes
out and the internal condenser LED turns on.
Fig. 5
Fig. 6
Fig. 7
Fig. 8
DF
BF
②

Page 9
Field Diaphragm (Optional)
1. Unscrew the lens ath the base of the microscope.
(Fig. 9)
• It may take a little bit of force to unscrew the
lens.
2. Fully screw the eld diaphragm (M-156).
3. System is ready for the use.
Fig. 9

Page 10
8. Summaryofbrighteldobservationprocedures
Main switch /
brightness adjustment knob
(Used commands ) (Chapter)
Slide holder
Nosepiece
Observation head
Diopter adjustment ring
Condenser centering
Aperture diaphragm
Field diaphragm
Nosepiece
Main switch /
brightness adjustment knob
Coarse and fine focus knobs
Coarse and fine focus knobs
Set the main swith to “ON” and adjust light intensity
Place a slide on the stage
Insert 10x objective into the light path
Focus the specimen
Begin observation
Adjust aperture and field diaphragms
Insert in the light path the desired objective and focus the
specimen
Adjust interpupillary distance
Adjust diopter
Center optical axis
Adjust brightness
10
10
5
5
5
5
10
10
10
10
10
5
10
X/Y movement knobs