Optika Italy IM Series User manual

Ver. 5.2 2021
Model
IM-3
IM-3F
IM-3FL4
IM-3LD2
IM-3LD4
IM-3LD4D
IM Series
INSTRUCTION MANUAL

Page 2
Table of Contents
1. Warning 4
2. Symbols and conventions 4
3. Safety Information 4
4. Intended use 4
5. Instrument description 5
5.1 IM-3 5
5.2 IM-3F 6
5.3 IM-3FL4 7
5.4 IM-3LD2 8
5.5 IM-3LD4 9
5.6 IM-3LD4D 10
6. Unpacking 12
6.1 IM-3 12
6.2 IM-3F / IM-3FL4 13
6.3 IM-3LD2 14
6.4 IM-3LD4 15
6.5 IM-3LD4D 16
7. Assembling 17
7.1 Installing the objectives 17
7.2 Installing stage extension or mechanical stage 17
7.3 Installing the stage insert 18
7.4 Installing the eyepieces 18
7.5 Installing the condenser 18
7.6 Installing the LED housing 19
7.7 Installingcolorlters 19
7.8 Installing the PC screen (IM-3LD4D) 20
7.9 Cable connection (IM-3LD4D) 20
7.10 Connecting the power supply 21
7.11 Installinguorescence 22
8. Brighteldobservation(transmittedlight) 26
9. Useofthemicroscopeinbrighteld(transmittedlight) 27
9.1 Turning on the microscope 27
9.2 Adjusting the light intensity 27
9.3 Adjusting the coarse focus tension 27
9.4 Diopter adjustment 27
9.5 Adjusting interpupillary distance 28
9.6 Use of eyeshields 28
9.7 Selecting the light path 28
9.8 Stage and stage inserts 29
9.8.1 Installing stage inserts 30
9.9 Aperture diaphragm 30
9.10 Usingcolorlters 31
10. Use of the microscope in phase contrast (transmitted light) 32
10.1 Installing the phase contrast slider 32
10.2 Phase contrast slider 32
10.3 Centering the phase ring 32
11. Use of the microscope in RPC (transmitted light) 34
11.1 Installing the RPC slider 34
11.2 RPC slider 34
11.3 RPC observation 35
12. Fluorescence observation (IM-3F / IM-3FL4) 36
13. Fluorescence observation (IM-3LD2 / IM-3LD4 / IM-3LD4D) 36
14. Use of the microscope inuorescence(reectedlight) 37
14.1 Centering the mercury bulb 37
14.2 Centeringtheelddiaphragm 39
14.3 Using the microscope 39
14.3.1Switchinguorescenceltercubes 39
14.3.2Availableuorescenceltercubes 40
14.4 Installinguorescencelter 41

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14.5 Use of the Anti-glow cap 42
14.6 Filter holder / Shutter 42
14.6.1InsertingaNDlter 42
14.6.2Usingthelterslider 43
15. Simultaneous observation in Phase Contrast + Fluorescence 44
15.1 IM-3F / IM-3FL4 44
15.2 IM-3LD2 / IM-3LD4 / IM-3LD4D 44
16. Microphotography 45
16.1 Use of C-mount cameras 45
16.2UseofReexcameras 45
17. Maintenance 46
18. Troubleshooting 47
Equipment disposal 49

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1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of mainte-
nance. It is built to high optical and mechanical standards and to withstand daily use. We remind you that this
manual contains important information on safety and maintenance, and that it must therefore be made accessi-
ble to the instrument users. We decline any responsibility deriving from incorrect instrument use uses that does
not comply with this manual.
2. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
3. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the op-
eration voltage of the equipment and that the lamp switch is in off position. Users should observe all safety reg-
ulations of the region. The equipment has acquired the CE safety label. However, users have full responsibility
to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure
safe operation of the unit.
4. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the sub-
ject.

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FILTER HOLDER
PHASE
CONTRAST SLIDER
PHOTO/TV
PORT
DIOPTER
ADJUSTMENT
RING
EYEPIECE
LED
HOUSING
CONDENSER
COARSE
FOCUS KNOB
FINE FOCUS KNOB
TENSION
ADJUSTMENT
COLLAR
OBJECTIVE
STAGE
MICROSCOPE
BODY
LIGHT PATH
SELECTOR
LEVER
STAGE
PLATE
5. Instrument description
5.1 IM-3
MAIN SWITCH

Page 6
PHOTO/TV
PORT
DIOPTER
ADJUSTMENT
RING
EYEPIECE
PHASE CONTRAST SLIDER
LED
HOUSING
CONDENSER
FILTER HOLDER
COARSE
FOCUS KNOB
FINE FOCUS KNOB
FLUORESCENCE
POWER SUPPLY
TENSION
ADJUSTMENT
COLLAR
OBJECTIVE
STAGE
MICROSCOPE
BODY
LIGHT PATH
SELECTOR
LEVER
STAGE PLATE
FLUORESCENCE LAMP
HOUSING
UV SHIELD
5.2 IM-3F
FLUORESCENCE
CUBE LEVER
MAIN SWITCH

Page 7
5.3 IM-3FL4
PHOTO/TV
PORT
DIOPTER
ADJUSTMENT
RING
EYEPIECE
LED
HOUSING
CONDENSER
FILTER HOLDER
COARSE
FOCUS KNOB
FINE FOCUS
KNOB
FLUORESCENCE
POWER SUPPLY
MICROSCOPE
BODY
LIGHT PATH
SELECTOR
LEVER
STAGE
PLATE
FLUORESCENCE LAMP
HOUSING
UV SHIELD
STAGE
FLUORESCENCE
CUBE LEVER
TRANSMITTED LIGHT
ADJUSTMENT KNOB

Page 8
DIOPTER
ADJUSTMENT
RING
EYEPIECE
PHASE
CONTRAST
SLIDER
LED
HOUSING
FILTER HOLDER
COARSE
FOCUS KNOB
FINE FOCUS KNOB
OBJECTIVE
STAGE
MICROSCOPE
BODY
LIGHT PATH
SELECTOR
LEVER
STAGE PLATE
CONDENSER
PHOTO/TV
PORT
5.4 IM-3LD2
FLUORESCENCE
CUBE LEVER
TRANSMITTED LIGHT
ADJUSTMENT KNOB
REFLECTED LIGHT
ADJUSTMENT KNOB

Page 9
5.5 IM-3LD4
DIOPTER
ADJUSTMENT
RING
EYEPIECE
LED
HOUSING
FILTER HOLDER
COARSE
FOCUS KNOB
FINE FOCUS KNOB
OBJECTIVE
STAGE
MICROSCOPE
BODY
LIGHT PATH
SELECTOR
LEVER
STAGE PLATE
CONDENSER
PHOTO/TV
PORT
FLUORESCENCE
CUBE LEVER
TRANSMITTED LIGHT
ADJUSTMENT KNOB
REFLECTED LIGHT
ADJUSTMENT KNOB
X-Y MOVEMENT
KNOBS

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5.6 IM-3LD4D
DIOPTER
ADJUSTMENT
RING
EYEPIECE
FILTER HOLDER
COARSE
FOCUS KNOB
FINE FOCUS KNOB
OBJECTIVE
STAGE
MICROSCOPE
BODY
LIGHT PATH
SELECTOR
LEVER
STAGE PLATE
CONDENSER
FLUORESCENCE
CUBE LEVER
TRANSMITTED LIGHT
ADJUSTMENT KNOB
REFLECTED LIGHT
ADJUSTMENT KNOB
USB CAMERA
MONITOR
X-Y MOVEMENT
KNOBS

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IM-3LD4D (back side)
MONITOR
POWER
SUPPLY
CABLE
HDMI PLUG
PC STICK
HDMI CABLE
(TO MONITOR)
USB CABLE
(TO CAMERA)
MONITOR
POWER
SUPPLY
CONNECTOR
PC STICK
POWER
SUPPLY
CABLE
LED
POWER
SUPPLY
CABLE
USB
MOUSE / KEYBOARD
RECEIVER

Page 12
6. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container
and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)
fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope
from the container and put it on a stable desk.
Once opened the box, the microscope parts are the following:
6.1 IM-3
① Microscopebody
② Condenser
③ Filterholder
④ LEDilluminator
⑤ Phasecontrastslider
⑥ Eyepieces
⑦ Centeringtelescope
⑧ Metalinsertforstage
⑨ Glassinsertforstage
⑩ Objectives
⑪ Powersupply
⑫ GreenlterIF550
⑪
⑫⑩
③④
⑤
⑥
⑦⑧ ⑨
①②

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⑲
① Microscopebody
② Condenser
③ LEDilluminator
④ Fluorescencepowersupply+cable
⑤ Fluorescencepowercable
⑥ Filterholder
⑦ Metalinsertforstage
⑧ Glassinsertforstage
⑨ Objectives
⑩ Eyepieces
⑪ GreenlterIF550
⑫ UVshield
⑬ Fluorescenceilluminator
⑭ HBOlamphousing
⑮ Microscopepowersupply
⑯ Phasecontrastslider
• ProvidedwithIM-3F
• OptionalwithIM-3FL4
⑰ Centeringtelescope
• ProvidedwithIM-3F
• OptionalwithIM-3FL4
⑱ Anti-glowcap
①
②
⑪
⑫ ⑬
⑭
⑮
⑩
③
④
⑤⑥
⑦ ⑧
⑨
6.2 IM-3F / IM-3FL4
⑯
⑰⑱

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6.3 IM-3LD2
① Microscopebody
② Condenser
③ LEDilluminator
④ Anti-glowcap
⑤ Mechanicalstage
⑥ Phasecontrastslider
⑦ Glassinsertforstage
⑧ Metalinsertforstage
⑨ Objectives
⑩ Eyepieces
⑪ Stageinserts
⑫ Centeringtelescope
⑬ GreenlterIF550
⑭ Lenscleaningtissue
⑮ UVshield
⑯ Dustcover
⑰ Powersupply+powercord
③
①
②
⑪⑩
④
⑤
⑥
⑦ ⑨
⑧
⑫⑬
⑭
⑮
⑯⑰

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6.4 IM-3LD4
① Microscopebody
② Condenser
③ LEDilluminator
④ Objectives
⑤ Eyepieces
⑥ Anti-glowcap
⑦ Glassinsertforstage
⑧ Metalinsertforstage
⑨ Mechanicalstage
⑩ UVshield
⑪ Stageinserts
⑫ Lenscleaningtissue
⑬ Dustcover
⑭ Powersupply+powercord
①
②
⑪
⑩
③
④
⑤
⑥
⑦
⑧
⑨
⑫
⑬
⑭

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6.5 IM-3LD4D
① Microscopebody
② Condenser
③ LEDilluminator
④ Anti-glowcap
⑤ Objectives
⑥ Eyepieces
⑦ Glassinsertforstage
⑧ Metalinsertforstage
⑨ Mechanicalstage
⑩ Stageinserts
⑪ Camera
⑫ “C”mount
⑬ Mouse
⑭ Keyboard
⑮ Monitor
⑯ UVshield
⑰ Powersupply+powercord
⑱ Micrometricslide
⑲ Lenscleaningtissue
⑳ Dustcover
①
②
⑪
⑫
③
④
⑤⑥
⑦⑧
⑨
⑩
⑬
⑭⑮⑯
⑰
⑱
⑲
⑳

Page 17
7. Assembling
FFig. 1ig. 1
①
②
FFig. 2ig. 2
FFig. 3ig. 3
③
FFig. 4ig. 4
7.1 Installing the objectives
1. Turn the coarse focusing knob ① until the nosepiece
reaches its lowest position.
• For a safe transport, the nosepiece is placed in the
lowest position and the tension adjustment collar
②is adjusted to the proper tension when the mi-
croscope leaves the factory. (Fig.1)
2. Screw the lowest magnication objective on the nose-
piece from the right side, then turn the nosepiece clock-
wise. Mount the other objectives in the same way, fol-
lowing the sequence from low to high.
• Note: the objectives can also be installed through
the stage opening. (Fig. 2)
• Clean the objectives regularly. In inverted microscopes,
the objectives are very sensitive to dust.
• To prevent dust and contamination from entering the
microscope, cover all the unused holes with dust caps
③. (Fig. 3)
• When operating, use the low power objective (10X) to
search and focus the specimen, then switch to higher
magnications.
• When switching between objectives, slowly turn the
nosepiece until it clicks. The click means that the objec-
tive is in the right position, in the center of the light path.
7.2 Installing stage extension or mechanical stage
• Stage extension and mechanical stage are optional
accessories for some models.
• Stage extension can be installed on either side of the
stage to enlarge the working surface.
• Mechanical stage can only be installed on the right side
of the stage.
1. Installing the units: screw the bolts in the xing holes
of the attachments, then mount the unit from below the
stage. (Fig. 4)

Page 18
FFig. 5ig. 5
FFig. 6ig. 6
FFig. 7ig. 7
7.3 Installing the stage insert
1. When using the glass stage, make sure that the insert
is horizontal.
2. Install the stage insert in the stage opening. (Fig. 5)
7.4 Installing the eyepieces
Insert both eyepieces into the tubes of the optical head.
(Fig. 6)
7.5 Installing the condenser
1. Insert the condenser illumination unit into the bracket.
(Fig. 7)
2. Turn the condenser illumination unit clockwise about
90°, with the “AS” mark of lter holder facing front. Align
the screw of the condenser illumination unit and the
hole of the holder, then screw the bolt in the hole with
the supplied Allen wrench. (Fig. 8)
FFig. 8ig. 8

Page 19
7.6 Installing the LED housing
• All models except IM-3LD4D
1. Insert the connector plug into the connector jack.
2. Push the LED housing gently into the holes of the illu-
mination unit. (Fig. 9)
• IM-3LD4D
The LED of the IM-3LD4D is already pre-installed on the
illuminator. No operation is therefore necessary. (Fig. 10)
7.7 Installingcolorlters
• Removethelterholder,theninstallthecolorlter
you need. (Fig. 11 - 12)
1. Mount the color lter at as shown in ①, checking that
they are not tilted.
• Ifthecolorlteristiltedorotherwiseoutofplace
②, it may fall.
• Color lters can be stacked in the holder. This allows to
install as many lters as needed, as long as the whole
thickness is less than 11 mm.
FFig. 9ig. 9
FFig. 10ig. 10
FFig. 12ig. 12
FFig. 11ig. 11
②
①

Page 20
FFig. 13ig. 13
FFig. 14ig. 14
FFig. 15ig. 15
7.8 Installing the PC screen (IM-3LD4D)
• For the camera installation, please refer to chapter 15.1.
1. Align the notch ① with the bracket ② on the illuminator.
(Fig. 13)
2. Once the monitor is fully inserted, tighten the wheel ③
to lock the monitor. (Fig.14)
7.9 Cable connection (IM-3LD4D)
1. Connect the USB mouse / keyboard receiver in the slot
“A” of the PC stick.
2. Plug the USB3.0 cable in the slot “B” of the PC stick.
3. Plug the PC stick power supply cable in the slot “C”.
4. Plug the HDMI cable in the slot “D” of the PC stick. (Fig.
15)
5. Plug the microscope LED power supply in the connec-
tor “E” of the microscope illuminator. (Fig. 16)
FFig. 16ig. 16
②
①
③