Optika Italy B-1000 Series User manual

Ver. 2.1 2022
Model
B-1000METBF
B-1000METDK
B-1000 Series
INSTRUCTION MANUAL

Page 2
Table of Contents
1. Warning 3
2. Safety Information 3
3. Package content 4
3.1 B-1000METBFTransmittedandreectedlight 4
3.2 B-1000METBFReectedlight 5
3.3 B-1000METDKTransmittedandreectedlight 6
3.4 B-1000METDKReectedlight 7
4. Unpacking 8
5. Intendeduse 8
6. Symbolsandconventions 8
7. Instrumentdescription 9
7.1 B-1000METBFTransmittedandreectedlight 9
7.2 B-1000METBFReectedlight 10
7.3 B-1000METDKTransmittedandreectedlight 12
7.4 B-1000METDKReectedlight 13
7.5 Motorisedversion 15
8. Assembling 16
8.1 B-1000METBF 16
8.2 B-1000METDK 19
8.2.1 Useofnosepieceadapterrings 19
8.3 Motorisedversion 20
9. Transmittedlightobservationprocedures 21
10. Useofthemicroscope(transmittedlight) 22
10.1 Generalswitchon 22
10.2 Control keyboard 22
10.3 Brightnessadjustment 22
10.4 Adjusttheobservationhead 22
10.5 Adjusttheinterpupillarydistance 23
10.6 Diopteradjustment 23
10.7 Useofeyeshields 23
10.8 Lightpathselection 24
10.9 Coarsefocustensionadjustment 24
10.10Focusstoplever 24
10.11Stage 25
10.12Centeringthecondenser 25
10.13E󰀨ectofelddiaphragm 25
10.14Aperturediaphragm 25
10.15Motorisedversion 26
10.15.1 Focusing 26
10.15.2 Stage 26
11. Brightfieldreflectedlightobservationprocedures 27
12. Useofthemicroscope(reflectedlight) 28
12.1 Generalswitchon 28
12.2 Brightnessadjustment 28
12.3 SwitchingBrighteld/Darkeldobservation 28
12.4 UseofNDlter 29
12.5 Changingthestageheight 29
12.6 Centeringtheelddiaphragm(FS) 29
12.7 E󰀨ectsoftheelddiaphragm 30
12.8 Centeringtheaperturediaphragm(AS) 30
12.9 Useofcolorlters 30
13. UseofthemicroscopeinPolarizedlight(PO) 31
13.1 Useofpolarizedlight 31
13.2 Use of connection plate 32
14. Microphotography 33
14.1 UseofC-mountcameras 33
14.2 Useofreexcameras 33
15. Maintenance 34
16. Troubleshooting 35
Equipmentdisposal 37

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of maintenance. It
is built to high optical and mechanical standards and to withstand daily use. We remind you that this manual contains
important information on safety and maintenance, and that it must therefore be made accessible to the instrument users.
We decline any responsibility deriving from incorrect instrument use uses that does not comply with this manual.
2. Safety Information
  AvoidingElectricalShock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the operation voltage
of the equipment and that the lamp switch is in o󰀨 position. Users should observe all safety regulations of the region. The
equipment has acquired the CE safety label. However, users have full responsibility to use this equipment safely. Please
follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit.

Page 4
①Frame
② Reectedlightilluminator
③ Observationhead
④ Eyepieces
⑤ Stage
⑥ Objectives
⑦ Condenser
⑧ Polarizer+Analyzer
⑨ ColorFilters
⑩ Powersupply
⑪ Allenwrench
⑫ Dustcover
⑬ Powercord
①
③
④⑤
⑥
⑦⑧
⑨
⑩⑪
②
⑫
⑬
3. Package content
3.1 B-1000METBFTransmittedandreectedlight

Page 5
3.2 B-1000METBFReectedlight
④
⑤
⑥
⑦
⑧
⑨
⑩
⑪
①Frame
② Reectedlightilluminator
③ Observationhead
④ Eyepieces
⑤ Stage
⑥ Objectives
⑦ Polarizer+Analyzer
⑧ ColorFilters
⑨ Allenwrench
⑩ Dustcover
⑪ Powercord
①
②
③

Page 6
①Frame
② Reectedlightilluminator
③ Observationhead
④ Eyepieces
⑤ Stage
⑥ Objectives
⑦ Condenser
⑧ Polarizer+Analyzer
⑨ ColorFilters
⑩ Powersupply
⑪ Allenwrench
⑫ Dustcover
⑬ Powercord
⑭ Adapterringsfornosepiece
①
③
④⑤
⑥
⑦⑧
⑨
⑩⑪
②
⑫
⑬
3.3 B-1000METDKTransmittedandreectedlight
⑭

Page 7
④
⑤
⑥
⑦
⑧
⑨
⑩
⑪
①Frame
② Reectedlightilluminator
③ Observationhead
④ Eyepieces
⑤ Stage
⑥ Objectives
⑦ Polarizer+Analyzer
⑧ ColorFilters
⑨ Allenwrench
⑩ Dustcover
⑪ Powercord
⑫ Adapterringsfornosepiece
①
②
③
3.4 B-1000METDKReectedlight
⑫

Page 8
4. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container and lift the
top half of the container. Take some care to avoid that the optical items (objectives and eyepieces) fall out and get dam-
aged. Using both hands (one around the arm and one around the base), lift the microscope from the container and put it
on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or other
residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
5. Intendeduse
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the subject.
6. Symbolsandconventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.

Page 9
FOCUS LOCK
LEVER
NOSEPIECE
TV/PHOTO PORT
CONDENSER
CENTERING
KNOBS
REFLECTED
LIGHT
POWER
SUPPLY
OBJECTIVE
STAGE
REFLECTED LIGHT
ILLUMINATOR
7. Instrumentdescription
7.1 B-1000METBFTransmittedandreectedlight
FILTERS SLOT
MAIN SWITCH
CONTROL
KEYBOARD
CONDENSER
HEIGHT
ADJUSTMENT
KNOB
TRANSMITTED
LIGHT INTENSITY
ADJUSTMENT
DIAL
DIC PRISM
SLOT
CONDENSER
REFLECTED
LIGHT INTENSITY
ADJUSTMENT
DIAL
POLARIZER
SLOT

Page 10
7.2 B-1000METBFReectedlight
FOCUS LOCK
LEVER
NOSEPIECE
TV/PHOTO PORT
OBJECTIVE
STAGE
REFLECTED LIGHT
ILLUMINATOR
FILTERS SLOT
DIC PRISM
SLOT
REFLECTED
LIGHT INTENSITY
ADJUSTMENT
DIAL
POLARIZER
SLOT
REFLECTED
LIGHT
POWER
SUPPLY

Page 11
B-1000METBFBothVersions-Oppositeside
COARSE
FOCUSING KNOB
DIOPTER
ADJUSTMENT
RING
EYEPIECE
APERTURE
DIAPHRAGM
FIELD DIAPHRAGM
FINE FOCUSING KNOB
LIGHT PATH
SELECTOR
LEVER
X-Y STAGE
MOVEMENT
KNOBS
TENSION
ADJUSTMENT
RING
ND FILTER LEVER
ANALYZER
SLOT

Page 12
7.3 B-1000METDKTransmittedandreectedlight
FOCUS LOCK
LEVER
NOSEPIECE
STAGE
REFLECTED LIGHT
ILLUMINATOR
DIC PRISM
SLOT
REFLECTED
LIGHT INTENSITY
ADJUSTMENT
DIAL
REFLECTED
LIGHT
POWER
SUPPLY
CONTROL
KEYBOARD
OBJECTIVE
DIOPTER
ADJUSTMENT
RING
EYEPIECE
TRANSMITTED
LIGHT INTENSITY
ADJUSTMENT DIAL
BF/DF SELECTION
LEVER
POLARIZER
SLOT
MAIN SWITCH
CONDENSER
HEIGHT
ADJUSTMENT
KNOB
CONDENSER
CENTERING
KNOBS
CONDENSER

Page 13
7.4 B-1000METDKReectedlight
FOCUS LOCK
LEVER
NOSEPIECE
OBJECTIVE
STAGE
DIC PRISM
SLOT
REFLECTED
LIGHT INTENSITY
ADJUSTMENT
DIAL
REFLECTED
LIGHT
POWER
SUPPLY
REFLECTED LIGHT
ILLUMINATOR
DIOPTER
ADJUSTMENT
RING
EYEPIECE
BF/DF SELECTION
LEVER
POLARIZER
SLOT

Page 14
B-1000METDKBothVersions-Oppositeside
TV/PHOTO PORT
LIGHT PATH
SELECTION LEVER
FILTERS SLOT
ND FILTER
LEVER
COARSE
FOCUSING KNOB
FINE FOCUSING KNOB
X-Y STAGE
MOVEMENT
KNOBS
TENSION
ADJUSTMENT
RING
ANALYZER
SLOT
APERTURE
DIAPHRAGM
FIELD DIAPHRAGM

Page 15
7.5 Motorisedversion
Only the parts related to the motorized version are highlighted.
X-AXIS MOVEMENT
KNOB
(MANUAL MOVEMENT)
FOCUS
MOTOR
STAGE
CONNECTION
CABLE
Y-AXIS MOVEMENT
KNOB
(MANUAL MOVEMENT)

Page 16
FFig. 1ig. 1
FFig. 2ig. 2
FFig. 4ig. 4
FFig. 3ig. 3
①
②
③
④
⑤
8. Assembling
8.1 B-1000METBF
1. Insert the reected light illuminator ①on the frame and lock
the locking knob ② with the provided Allen wrench. (Fig. 1)
2. Remove the cover ③on the back side of the illuminator. (Fig.
2)
3. Insert the LED housing ④and lock the locking screws ⑤.
(Fig. 3-4)

Page 17
4. Connect the illuminator cable ⑥to the connector ⑦placed
on the back side of the power supply. (Fig. 5)
FFig. 5ig. 5
⑥
⑦
FFig. 6ig. 6
⑧
5. Connect the power supply to the connector ⑧placed on the
back side of the power supply. (Fig. 6)
6. Insert the optical head above the illuminator and tighten the
locking screw ⑨ with the provided Allen wrench. (Fig. 7)
• Holdtheheadwithonehandduringthelockinginorder
toavoidthattheheadfalls.
7. Insert both eyepieces into the tubes of the optical head. (Fig.
8)
FFig. 7ig. 7
FFig. 8ig. 8
⑨

Page 18
FFig. 10ig. 10
FFig. 11ig. 11
8. Screw each objective into the thread of the nosepiece,
clockwise with increasing magnication. (Fig. 9)
⑩
FFig. 9ig. 9
FFig. 12ig. 12
⑪
9. Mount the stage: lower the support using the coarse focus
knob, then place the stage and rmly tighten the lock screw
⑩. (Fig. 10)
Onlyfortransmittedandreectedlightversion:
10. Insert the condenser under the stage: position until it is well
inserted into its holder (under the condenser there is a pin
that must fully enter the holder guide). (Fig. 11)
11. Lock the condenser xing knob ⑪.
12. Insert the power supply jack in the socket placed in the rear
side of the of the microscope. (Fig. 12)

Page 19
8.2 B-1000METDK
• The reected light illuminator for the B-1000METDK is in-
stalled prior to shipment from the factory and requires no
special installation procedures.
Repeat steps from 2. to 12. of chapter 8.1 for the microscope
assembling.
8.2.1 Use of nosepiece adapter rings
The nosepiece can allocate objectives for brighteld/darkeld
observation (BD).
However, it is possible to install objectives for brighteld using
the adapter rings.
1. Screw the adapter ring into the empty hole of the nosepiece.
(Fig. 13-14)
FFig. 13ig. 13
FFig. 14ig. 14
FFig. 15ig. 15
2. Screw the objective into the adapter ring. (Fig. 15)
3. Perform the observation as usual.
• The brighteld objective cannot be used for darkeld,
therefore switching the mirror cube selector to “DF” could
bring to a completely dark image.
• Using the adapter rings it could happen an imperfect
parfocalitybetweenbrighteldand brighteld/darkeld
objectives.Thisisnotadefect.

Page 20
8.3 Motorisedversion
1. Assemble the stage in the same way as the manual version.
• Check the perfect alignment of the rear part of the stage with
the rear arm of the frame.
• A non perfect alignment could lead to an incorrect functioning
of the system. (Fig. 16)
FFig. 16ig. 16
FFig. 17ig. 17
②
①
FFig. 18ig. 18
③ ④ ⑤⑥
2. Connect the cable ① from the stage to the frame and tight
the locking screws of the connectors ②. (Fig. 17)
3. Connect the provided cables: ③ 12V power supply for the
motorised parts; ④ 6V microscope power supply; ⑤ serial
cable; ⑥ PS/2 mouse. (Fig. 18)
• Connectpowercablesasthelaststep.