Optika Italy B-150D-MRPL User manual

Model
B-150D-MRPL
B-150D-BRPL
B-150D Series
INSTRUCTION MANUAL
Ver. 2.0 2019

Page 2
1. Warning 3
2. Symbols and conventions 3
3. Safety Information 3
4. Intended use 3
5. Overview 4
5.1 B-150D-MRPL 4
5.2 B-150D-BRPL 5
6. Unpacking 6
7. Assembling 6
7.1 B-150D-MRPL 6
7.2 B-150D-BRPL 7
7.3 Assembling the microscope 8
7.4 Polarizing set (optional) 9
8. Use of the microscope 10
8.1 Light intensity adjustment 10
8.2 Coarse focus tension adjustment 10
8.3 Stage 10
8.4 Adjust the interpupillary distance 10
8.5 Diopter adjustment 11
8.6 Use of oil immersion objective 11
8.7 Aperture diaphragm 12
8.8 Use with rechargeable batteries 12
8.9 Use of the polarizer (optional) 12
9. Maintenance 13
10. Troubleshooting 14
Equipment disposal 15
Summary

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of
maintenance. It is built to high optical and mechanical standards and to withstand daily use. We remind you
that this manual contains important information on safety and maintenance, and that it must therefore be made
accessible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses
that does not comply with this manual.
2. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
3. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the
operation voltage of the equipment and that the lamp switch is in o󰀨 position. Users should observe all safety
regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibility
to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure
safe operation of the unit.
4. Intended use
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.

Page 4
COARSE
FOCUS KNOB
FINE FOCUS KNOB
EYEPIECE
OBSERVATION
HEAD
MAIN SWITCH / INTENSITY
ADJUSTMENT DIAL
CONDENSER
STAGE
LED CHARGE
INDICATOR
NOSEPIECE
OBJECTIVES
TENSION
ADJUSTMENT
COLLAR
SLIDE HOLDER
X/Y MOVEMENT
KNOBS
5. Overview
5.1 B-150D-MRPL

Page 5
5.2 B-150D-BRPL
COARSE
FOCUS KNOB
FINE FOCUS KNOB
EYEPIECES
OBSERVATION
HEAD
MAIN SWITCH / INTENSITY
ADJUSTMENT DIAL
CONDENSER
STAGE
LED CHARGE
INDICATOR
NOSEPIECE
OBJECTIVES
TENSION
ADJUSTMENT
COLLAR
SLIDE HOLDER
X/Y MOVEMENT
KNOBS

Page 6
6. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container
and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)
fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope
from the container and put it on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or other
residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
7. Assembling
Once opened the box, the microscope parts are the following:
7.1 B-150D-MRPL
① Frame
② Monocular observation head
③ Eyepiece
④ Tension adjustment tool
⑤ Objectives (4X / 10X / 40X)
⑥ Dust cover
⑦ Green lter
⑧ Frosted lter
⑨ Power supply
⑩ USB cable
③
④
⑤
⑥
⑦⑧
①
②⑨
⑩

Page 7
7.2 B-150D-BRPL
③
④
⑤
⑥
⑦ ⑧
①
②
① Frame
② Binocular observation head
③ Eyepieces
④ Tension adjustment tool
⑤ Objectives (4X / 10X / 40X / 100X)
⑥ Dust cover
⑦ Green lter
⑧ Frosted lter
⑨ Power supply
⑩ USB cable
⑪Immersion oil
⑨
⑩
⑪

Page 8
7.3 Assembling the microscope
1. Remove the dust cap from the microscope frame
and from the bottom of the observation head.
2. Insert the optical head above the stand and
tighten the screw ①. (Fig. 1)
• Hold the head with one hand during the lo-
cking in order to avoid that the head falls.
3. Insert both eyepieces into the tubes of the optical
head. (Fig. 2)
• For model B-150D-MRPL: only one eyepiece
is used as this model is monocular.
4. Insert the power supply jack in the socket placed
at the rear side of the microscope. (Fig. 3)
Fig. 2
Fig. 3
Fig. 1
①

Page 9
7.4 Polarizing set (optional)
1. Place the polarizer on the light exit ①at the
base of the microscope. (Fig. 4)
2. Loosen the head xing knob ② and remove
the head from the microscope frame. (Fig.
5)
3. Insert the analyzer into the hole inside the
frame ③. (Fig. 6)
4. Put back the head into its original position
and lock the xing knob.
Fig. 6
③
Fig. 4
①
Fig. 5
②

Page 10
8. Use of the microscope
8.1 Light intensity adjustment
Operate on the light intensity adjustment dial to turn
ON / OFF the microscope and to increase / decrease
the illumination voltage ①. (Fig. 7)
8.2 Coarse focus tension adjustment
• Adjust the tension using the provided tool.
The coarse knob tension is pre-setted in the factory.
To modify the tension according to personal’s needs,
rotate the ring ② using the provided tool (Fig. 8).
Clockwise rotation increases the tension. If the ten-
sion is too loose, the stage could go lower by itself
or the focus easily lost after ne adjustment. In this
case, rotate the knob in order to increase the tension.
8.3 Stage
Stage accepts standard slides 26 x 76 mm, thickness
1,2 mm with coverslide 0,17mm. (Fig. 9)
1. Open the spring arm of the slide holder ③ and
place the slide from the front on the stage.
2. Gently release the spring arm of the slide holder.
• A sudden release of the spring arm could cau-
se the falling of the slide.
8.4 Adjust the interpupillary distance
Only B-150D-BRPL
Hold the right and left parts of the observation head
using both hands and adjust the interpupillary distan-
ce by turning the two parts until one circle of light can
be seen. (Fig. 10)
• The graduation on the interpupillary distance in-
dicator ④, pointed by the spot “.” on the eyepiece
holder, shows the distance between the opera-
tor’s eyes.
The range of the interpupillary distance is 48- 75 mm.
Fig. 7
①
Fig. 8
②
Fig. 10
④
Fig. 9
③

Page 11
8.5 Diopter adjustment
Only B-150D-BRPL
1. Look into the right eyepiece with your right eye
only, and focus on the specimen.
2. Look into the left eyepiece with your left eye
only. If the image is not sharp, use the dioptric
adjustment ring ① to compensate. (Fig. 11)
• The adjustment range is ±5 diopter. The num-
ber indicated on the adjustment ring gradua-
tion should correspond to the operator’s dio-
ptric correction.
8.6 Use of oil immersion objective
Only B-150D-BRPL
1. Focus the specimen with a low power objective.
2. Lower the stage.
3. Put a drop of oil (provided) on the area of the spe-
cimen to be observed. (Fig. 12)
• Make sure that there are no oil bubbles. Air
bubbles in the oil damage the image quality.
• To check for bubbles: remove an eyepiece, ful-
ly open the aperture diaphragm and observe the
objective exit pupil. (The pupil must be circular
and bright).
• To remove the bubbles, gently move the nose-
piece to the right and left to move the immersion
objective a few times and allow the air bubbles to
move.
4. Insert immersion objective.
5. Return the stage to the upper focusing point and
obtain an optimal focus using the ne focus knob.
6. After use, gently remove the oil with a soft pa-
per towel or a lightly moistened optic paper with
a mixture of ethyl ether (70%) and absolute ethyl
alcohol (30%).
• The immersion oil, if not immediately cleaned,
could crystallize creating a glass-like layer.
In this situation the observation of the speci-
menwouldbedi󰀩cult(evennotimpossible)
due to the presence of an additional thickness
on the objective.
Fig. 11
①
Fig. 12

Page 12
8.7 Aperture diaphragm
The Numerical Aperture (N.A.) value of the aper-
ture diaphragm a󰀨ects the image contrast. Incre-
asing or reducing this value one can vary reso-
lution, contrast and depth of focus of the image.
Move the diaphragm lever ① (Fig. 13) toward left
or right to decrease or increase the N.A. value.
• With low contrast specimens set the numerical
aperture to about 70%-80% of the objective’s N.A.
If necessary, remove on eyepiece and, looking into
empty sleeve, adjust the condenser’s diaphragm
in order to obtain an image like the one in Fig. 14
8.8 Use with rechargeable batteries
When the microscope is plugged with the power
supply, the LED indicator for the battery recharge ②
is lit. (Fig. 15)
• LED red: battery under charge
• LED green: battery fully charged.
When the microscope is unplugged, the LED is o󰀨.
During the normal use with batteries, LED is always
OFF.
8.9 Use of the polarizer (optional)
1. Remove the specimen from the stage.
2. Looking inside the eyepieces, rotate the polarizer
until the darkest position is achieved.
3. Once the dark is achieved (“extinction” or “Cros-
sed Nicol” position) it is possible to begin the
observation.
Fig. 13
①
Fig. 14
IRIS DIAPHRAGM
FIELD OF VIEW
30-20%
70-80%
Fig. 15
②

Page 13
9. Maintenance
To think about when and after using the microscope
• The microscope should always be kept vertically when moving it and be careful so that no moving
parts, such as the eyepieces, fall out.
• Never mishandle or impose unnecessary force on the microscope.
• Never attempt to service the microscope yourself.
• After use, turn o󰀨 the light immediately, cover the microscope with the provided dust-cover, and keep
it in a dry and clean place.
Electrical safety precautions
• Before plugging in the power supply, make sure that the supplying voltage of your region matches
with the operation voltage of the equipment and that the lamp switch is in o󰀨-position.
• Users should observe all safety regulations of the region. The equipment has acquired the CE safety
label. However, users do have full responsibility to use this equipment safely.
Cleaning the optics
• If the optical parts need to be cleaned try rst to: use compressed air.
• If that is not su󰀩cient: use a soft lint-free piece of cloth with water and a mild detergent.
• And as a nal option: use the piece of cloth moistened with a 3:7 mixture of ethanol and ether.
• Note: ethanol and ether are highly ammable liquids. Do not use them near a heat source, near sparks or
near electric equipment. Use these chemicals in a well ventilated room.
• Remember to never wipe the surface of any optical items with your hands. Fingerprints can damage the
optics.
• Do not disassemble objectives or eyepieces in attempt to clean them.
For the best results, use the OPTIKA cleaning kit (see catalogue).
If you need to send the microscope to Optika for maintenance, please use the original packaging.

Page 14
10. Troubleshooting
Review the information in the table below to troubleshoot operating problems.
PROBLEM CAUSE SOLUTION
I. Optical Section:
LED operates, but eld of view re-
mains dark. Power supply is unplugged. Connect
Brightness is too low Set brightness to a proper level
Batteries are uncharged Fully charge the batteries
Dirt or dust is visible in the eld of
view. Dirt/dust on the specimen Clean the specimen
Dirt/dust on the eyepieces Clean the eyepieces
Image looks double Aperture diaphragm is stopped
down too far Open aperture diaphragm
Visibility is poor.
• Image is not good.
• Contrast is poor.
• Details are indistinct.
• Image glares
Revolving nosepiece is in an
incorrect position
Move the nosepiece to a click stop
Aperture diaphragm is too closed
or to open
Adjust aperture diaphragm
Dust or dirt on lenses (condenser,
objectives, eyepieces and slide) Clean thoroughly
One side of the image is out of focus. The nosepiece is not in the center
of the light path Turn the nosepiece to a click stop
The specimen is out of place
(tilted) Place the specimen at on the stage.
The optical performance of the
sample cover glass is poor Use a cover glass of better quality
II. Mechanical Section:
The coarse focus knob is hard to
turn. The tension adjustment collar is too tight
Loosen the tension adjustment collar
The focus is unstable. The tension adjustment collar is
too loose Tighten the tension adjustment collar
III. Electric section
The LED doesn’t turn on. No power supply Check the power cord connection
Batteries are uncharged Fully charge the batteries
The brightness is not enough The brightness adjustment is low Adjust the brightness
The light blinks The power cord is poorly con-
nected Check the power cord
IV. Observation tube
Field of view of one eye does not
match that of the other. Interpupillary distance is incorrect. Adjust interpupillary distance.
Incorrect diopter adjustment.Adjust diopter.
Your view is not accustomed to
microscope observation.
Upon looking into eyepieces, try looking at
overall field before concentrating on speci-
men range. You may also find it helpful to
look up and into distance for a moment
before looking back into microscope.

Page 15
Equipment disposal
Art.13 Dlsg 25 July 2005 N°151. “According to directives 2002/95/EC, 2002/96/EC and 2003/108/EC relating
to the reduction in the use of hazardous substances in electrical and electronic equipment and waste dispo-
sal.”
The basket symbol on equipment or on its box indicates that the product at the end of its useful life should be
collected separately from other waste. The separate collection of this equipment at the end of its lifetime is or-
ganized and managed by the producer. The user will have to contact the manufacturer and follow the rules that
he adopted for end-of-life equipment collection. The collection of the equipment for recycling, treatment and
environmentally compatible disposal, helps to prevent possible adverse e󰀨ects on the environment and health
and promotes reuse and/or recycling of materials of the equipment. Improper disposal of the product involves
the application of administrative penalties as provided by the laws in force.

OPTIKA S.r.l.
Via Rigla, 30 - 24010 Ponteranica (BG) - ITALY Tel.: [phone]
[email protected] - www.optikamicroscopes.com
OPTIKA Spain
[email protected]
OPTIKA USA
[email protected]
OPTIKA China
[email protected]
OPTIKA India
[email protected]
OPTIKA Central America
[email protected]
®
®
®
®
®
®

Modelli
B-150D-MRPL
B-150D-BRPL
Serie B-150D
MANUALE DI ISTRUZIONI
Ver. 2.0 2019

Pagina 18
1. Avvertenza 19
2. Simboli 19
3. Informazioni sulla sicurezza 19
4. Utilizzo previsto 19
5. Descrizione dello strumento 20
5.1 B-150D-MRPL 20
5.2 B-150D-BRPL 21
6. Disimballaggio 22
7. Assemblaggio 22
7.1 B-150D-MRPL 22
7.2 B-150D-BRPL 23
7.3 Procedura di assemblaggio 24
7.4 Set di polarizzazione (opzionale) 25
8. Uso del microscopio 26
8.1 Regolazione intensità luminosa 26
8.2 Regolazione della frizione 26
8.3 Tavolino 26
8.4 Regolazione distanza interpupillare 26
8.5 Regolazione diottrica 27
8.6 Uso di obiettivi ad immersione 27
8.7 Diaframma di apertura 28
8.8 Uso con batterie ricaricabili 28
8.9 Uso con polarizzatore (opzionale) 28
9. Manutenzione 29
10. Risoluzione dei problemi 30
Smaltimento 31
Sommario

Pagina 19
1. Avvertenza
Questo microscopio è uno strumento scientico di alta precisione, progettato per durare a lungo con una minima
manutenzione; la realizzazione è secondo i migliori standard ottici e meccanici, per poter essere utilizzato
quotidianamente. Vi ricordiamo che questo manuale contiene informazioni importanti per la sicurezza e per la
manutenzione dello strumento, e deve quindi essere messo a disposizione di coloro che lo utilizzeranno.
Decliniamo ogni responsabilità derivante da un utilizzo dello strumento non indicato nel presente manuale.
2. Simboli
La seguente tabella riporta i simboli utilizzati in questo manuale.
PERICOLO
Questo simbolo indica un rischio potenziale ed avverte di procedere con cautela.
SHOCK ELETTRICO
Questo simbolo indica un rischio di shock elettrico.
3. Informazioni sulla sicurezza
Per evitare shock elettrici
Prima di collegare il cavo di alimentazione alla presa elettrica, assicurarsi che il voltaggio della rete locale
coincida con il voltaggio dello strumento e che l’interruttore dell’illuminazione sia nella posizione “OFF”.
Gli utenti dovranno seguire tutte le norme di sicurezza locali. Lo strumento è certicato CE. In ogni caso, gli
utilizzatori sono gli unici responsabili per un utilizzo sicuro dello strumento. Per l’utilizzo in sicurezza dello
strumento è importante attenersi alle seguenti istruzioni e leggere il manuale in tutte le sue parti.
4. Utilizzo previsto
Solo per applicazioni di ricerca ed usi didattici. Non indicato per utilizzo diagnostico e terapeutico umano e
veterinario.

Pagina 20
5. Descrizione dello strumento
5.1 B-150D-MRPL
MANOPOLA
MACROMETRICA DI
MESSA A FUOCO
MANOPOLA
MICROMETRICA DI
MESSA A FUOCO
OCULARE
TESTA DI
OSSERVAZIONE
INTERRUTTORE / ROTELLA
REGOLAZIONE INTENSITÀ
CONDENSATORE
TAVOLINO
LED INDICATORE
DI CARICA
REVOLVER
OBIETTIVI
ANELLO
REGOLAZIONE
TENSIONE
FERMAVETRINO
MANOPOLE
TRASLAZIONE X/Y