Optika Italy B-150P Series User manual

Model
B-150P-MRPL
B-150P-BRPL
B-150P Series
INSTRUCTION MANUAL
Ver. 5.0 2019

Page 2
1. Warning 3
2. Symbols and conventions 3
3. Safety Information 3
4. Intended use 3
5. Overview 4
5.1 B-150P-MRPL 4
5.2 B-150P-BRPL 5
6. Unpacking 6
7. Assembling 6
7.1 B-150P-MRPL 6
7.2 B-150P-BRPL 7
7.3 Assembling the microscope 8
8. Use of the microscope 9
8.1 Light intensity adjustment 9
8.2 Coarse focus tension adjustment 9
8.3 Stage 9
8.4 Adjust the interpupillary distance 9
8.5 Diopter adjustment 10
8.6 Aperture diaphragm 10
8.7 Use with rechargeable batteries 10
8.8 Useinbrighteld 11
8.9 Use in polarized light 11
9. Maintenance 12
10. Troubleshooting 13
Equipment disposal 14
Table of Contents

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of
maintenance. It is built to high optical and mechanical standards and to withstand daily use. We remind you
that this manual contains important information on safety and maintenance, and that it must therefore be made
accessible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses
that does not comply with this manual.
2. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
3. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the ope-
ration voltage of the equipment and that the lamp switch is in o󰀨 position. Users should observe all safety regu-
lations of the region. The equipment has acquired the CE safety label. However, users have full responsibility to
use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure safe
operation of the unit.
4. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the
subject.

Page 4
5. Overview
COARSE
FOCUS KNOB
FINE FOCUS
KNOB
EYEPIECE
OBSERVATION
HEAD
MAIN SWITCH / INTENSITY
ADJUSTMENT DIAL
CONDENSER
STAGE
LED CHARGE
INDICATOR
NOSEPIECE
OBJECTIVES
TENSION
ADJUSTMENT
COLLAR
STAGE
CLIPS
POLARIZER
ANALYZER
5.1 B-150P-MRPL

Page 5
STAGE
CLIPS
5.2 B-150P-BRPL
LED CHARGE
INDICATOR
FINE FOCUS
KNOB
STAGE
OBSERVATION
HEAD
CONDENSER
OBJECTIVES
MAIN SWITCH / INTENSITY
ADJUSTMENT DIAL
NOSEPIECE
EYEPIECES
POLARIZER
ANALYZER
COARSE
FOCUS KNOB
TENSION
ADJUSTMENT
COLLAR

Page 6
6. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge
of the container and lift the top half of the container. Take some care to avoid that the opti-
cal items (objectives and eyepieces) fall out and get damaged. Using both hands (one around the
arm and one around the base), lift the microscope from the container and put it on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or
other residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
7. Assembling
Once opened the box, the microscope parts are the following:
7.1 B-150P-MRPL
① Frame
② Monocular observation head
③ Eyepiece
④ Tension adjustment tool
⑤ Objectives (4X / 10X / 40X)
⑥ Dust cover
⑦ Green lter
⑧ Power supply
③
④
⑤
⑥⑦⑧
①
②

Page 7
7.2 B-150P-BRPL
① Frame
② Monocular observation head
③ Eyepiece
④ Tension adjustment tool
⑤ Objectives (4X / 10X / 40X)
⑥ Dust cover
⑦ Green lter
⑧ Power supply
③
④
⑤
⑥⑦
⑧
①
②

Page 8
7.3 Assembling the microscope
1. Remove the dust cap from the microscope frame
and from the bottom of the observation head.
2. Insert the optical head above the stand and
tighten the screw. (Fig. 1)
•Hold the head with one hand during the lo-
cking in order to avoid that the head falls.
3. Insert both eyepieces into the tubes of the optical
head. (Fig. 2)
•For model B-150P-MRPL: only one eye-
piece is used as this model is monocular.
4. Insert the power supply jack in the socket placed
at the rear side of the microscope. (Fig. 3)
Fig. 1
Fig. 2
Fig. 3

Page 9
8. Use of the microscope
Fig. 6
Fig. 4
①
Fig. 5
②
Fig. 7
③
④
⑤
8.1 Light intensity adjustment
Operate on the light intensity adjustment dial ① to
turn ON / OFF the microscope and to increase / de-
crease the illumination voltage. (Fig. 4)
8.2 Coarse focus tension adjustment
• Adjust the tension using the provided tool.
The tension of the coarse focusing knob is factory
preset.
1. To modify the tension according to personal’s
needs, rotate the ring ② . (Fig. 5)
• Clockwise rotation increases the tension.
• If the tension is too loose, the stage could go
lower by itself or the focus easily lost after ne
adjustment. In this case, rotate the knob in order
to increase the tension .
8.3 Stage
Stage is equipped with two clips ③ for the sample
placement.
Standard specimen is glass slide, thickness 1,2 mm
with coverslide 0,17mm. (Fig. 6)
Specimen can be moved with hands.
1. Lift the clip, place the specimen and gently relea-
se the clip until the specimen is secured over the
stage.(Fig. 6)
2. When used in polarized light stage can be ro-
tated on 360°, be grabbing the knurled ring
④ placed on the outer side of the stage.
8.4 Adjust the interpupillary distance
Only B-150P-BRPL
Hold the right and left parts of the observation head
using both hands and adjust the interpupillary distan-
ce by turning the two parts until one circle of light can
be seen. (Fig. 7)
• The graduation on the interpupillary distance in-
dicator ⑤, pointed by the spot “.” on the eyepiece
holder, shows the distance between the opera-
tor’s eyes.
The range of the interpupillary distance is 48- 75 mm.

Page 10
8.5 Diopter adjustment
Only B-150P-BRPL
1. Look into the right eyepiece with your right eye
only, and focus on the specimen.
2. Look into the left eyepiece with your left eye
only. If the image is not sharp, use the dioptric
adjustment ring ① to compensate. (Fig. 8)
• The adjustment range is ±5 diopter. The num-
ber indicated on the adjustment ring gradua-
tion should correspond to the operator’s dio-
ptric correction.
8.6 Aperture diaphragm
• The Numerical Aperture (N.A.) value of the aper-
ture diaphragm a󰀨ects the image contrast. Incre-
asing or reducing this value one can vary reso-
lution, contrast and depth of focus of the image.
Move the diaphragm lever ② (Fig. 9) toward left
or right to decrease or increase the N.A. value.
• With low contrast specimens set the numerical
aperture to about 70%-80% of the objective’s
N.A. If necessary, remove on eyepiece and, lo-
oking into empty sleeve, adjust the condenser’s
diaphragm in order to obtain an image like the
one in Fig. 10
8.7 Use with rechargeable batteries
When the microscope is plugged with the power sup-
ply, the LED indicator for the battery recharge ③is
lit. (Fig. 14)
• LED red: battery under charge
• LED green: battery fully charged.
When the microscope is unplugged, the LED is o󰀨.
During the normal use with batteries, LED is always
OFF.
Fig. 8
Fig. 9
②
Fig. 10
APERTURE
DIAPHRAGM
FIELD OF VIEW
30-20%
70-80%
Fig. 11
③
①

Page 11
8.8 Useinbrighteld
1. Move the analyzer slider ① to the right to remove
analyzer lter from the light path.(Fig. 12)
• In this way it is possible to work in brighteld.
8.9 Use in polarized light
1. Move the analyzer slider ① to the left to insert
analyzer lter into the light path.(Fig. 12)
2. Remove one eyepiece from the observation head.
3. Insert 10X objective into the light path.
4. Remove the specimen from the stage.
5. Rotate the polarizer lter ②(Fig. 13) until a com-
plete dark eld of view can be achieved. Now the
“crossed-Nicol” position is obtained and it is pos-
sible work in polarized light.
6. Put a specimen on the stage.
7. Insert the desired objective.
8. Focus the specimen.
9. Begin the observation.
• Polarizer can be removed from the light path sim-
ply rotating the polarizer unit toward right. (Fig.
14)
Fig. 12
①
Fig. 13
②
Fig. 14

Page 12
9. Maintenance
Microscopy environment
This microscope is recommended to be used in a clean, dry and shock free environment with a temperature of
5°-40°C and a maximum relative humidity of 85 % (non condensing). Use a dehumidier if needed.
To think about when and after using the microscope
• The microscope should always be kept vertically when moving it and be careful so that no
moving parts, such as the eyepieces, fall out.
• Never mishandle or impose unnecessary force on the microscope.
• Never attempt to service the microscope yourself.
• After use, turn o󰀨 the light immediately, cover the microscope with the provided dust-cover,
and keep it in a dry and clean place.
Electrical safety precautions
•Before plugging in the power supply, make sure that the supplying voltage of your region
matches with the operation voltage of the equipment and that the lamp switch is in o󰀨-
position.
•Users should observe all safety regulations of the region. The equipment has acquired the
CE safety label. However, users do have full responsibility to use this equipment safely.
Cleaning the optics
•If the optical parts need to be cleaned try rst to: use compressed air.
•If that is not su󰀩cient: use a soft lint-free piece of cloth with water and a mild detergent.
•And as a nal option: use the piece of cloth moistened with a 3:7 mixture of ethanol and ether.
•Note: ethanol and ether are highly ammable liquids. Do not use them near a heat source, near
sparks or near electric equipment. Use these chemicals in a well ventilated room.
•Remember to never wipe the surface of any optical items with your hands. Fingerprints can damage the
optics.
•Do not disassemble objectives or eyepieces in attempt to clean them.
Forthebestresults,usetheOPTIKAcleaningkit(seecatalogue).
If you need to send the microscope to Optika for maintenance, please use the original packaging.

Page 13
10. Troubleshooting
Review the information in the table below to troubleshoot operating problems.
PROBLEM CAUSE SOLUTION
I. Optical Section:
LED operates, but eld of view remains
dark. Power supply is unplugged. Connect
Brightness is too low Set brightness to a proper level
Batteries are uncharged Fully charge the batteries
Dirt or dust is visible in the eld of view. Dirt/dust on the specimen Clean the specimen
Dirt/dust on the eyepieces Clean the eyepieces
Image looks double Aperture diaphragm is stopped down
too far Open aperture diaphragm
Visibility is poor.
• Image is not good.
• Contrast is poor.
• Details are indistinct.
• Image glares
Revolving nosepiece is in an incorrect
position
Move the nosepiece to a click stop
Aperture diaphragm is too closed or to
open
Adjust aperture diaphragm
Dust or dirt on lenses (condenser, ob-
jectives, eyepieces and slide) Clean thoroughly
Polarizing filters are not in “crossed-
Nicol” position Set them until “crossed-Nicol” position can be achieved
One side of the image is out of focus. The nosepiece is not in the center of
the light path Turn the nosepiece to a click stop
The specimen is out of place (tilted) Place the specimen at on the stage.
The optical performance of the sample
cover glass is poor Use a cover glass of better quality
II. Mechanical Section:
The coarse focus knob is hard to turn. The tension adjustment collar is too
tight
Loosen the tension adjustment collar
The focus is unstable. The tension adjustment collar is too
loose Tighten the tension adjustment collar
III. Electric section
The LED doesn’t turn on. No power supply Check the power cord connection
Batteries are uncharged Fully charge the batteries
The brightness is not enough The brightness adjustment is low Adjust the brightness
The light blinks The power cord is poorly connected Check the power cord
IV. Observation tube
Field of view of one eye does not match
that of the other. Interpupillary distance is incorrect. Adjust interpupillary distance.
Incorrect diopter adjustment.Adjust diopter.
Your view is not accustomed to micro-
scope observation.
Upon looking into eyepieces, try look-
ing at overall field before concentrating
on specimen range. You may also find
it helpful to look up and into distance
for a moment before looking back into
microscope.

OPTIKA S.r.l.
Via Rigla, 30 - 24010 Ponteranica (BG) - ITALY Tel.: [phone]
[e-mail] - www.optikamicroscopes.com
OPTIKA Spain
[e-mail]
OPTIKA USA
[e-mail]
OPTIKA China
[e-mail]
OPTIKA India
[e-mail]
OPTIKA Central America
[e-mail]
®
®
®
®
®
®
Page 14
Equipment disposal
Art.13 Dlsg 25 July 2005 N°151. “According to directives 2002/95/EC, 2002/96/EC and 2003/108/EC relating
to the reduction in the use of hazardous substances in electrical and electronic equipment and waste disposal.”
The basket symbol on equipment or on its box indicates that the product at the end of its useful life should be
collected separately from other waste. The separate collection of this equipment at the end of its lifetime is or-
ganized and managed by the producer. The user will have to contact the manufacturer and follow the rules that
he adopted for end-of-life equipment collection. The collection of the equipment for recycling, treatment and
environmentally compatible disposal, helps to prevent possible adverse e󰀨ects on the environment and health
and promotes reuse and/or recycling of materials of the equipment. Improper disposal of the product involves
the application of administrative penalties as provided by the laws in force.

OPTIKA S.r.l.
Via Rigla, 30 - 24010 Ponteranica (BG) - ITALY Tel.: [phone]
[email protected] - www.optikamicroscopes.com
OPTIKA Spain
[email protected]
OPTIKA USA
[email protected]
OPTIKA China
[email protected]
OPTIKA India
[email protected]
OPTIKA Central America
[email protected]
®
®
®
®
®
®

Modelli
B-150P-MRPL
B-150P-BRPL
Serie B-150P
MANUALE DI ISTRUZIONI
Ver. 5.0 2019

Pagina 17
1. Avvertenza 18
2. Simboli 18
3. Informazioni sulla sicurezza 18
4. Utilizzo previsto 18
5. Descrizione dello strumento 19
5.1 B-150P-MRPL 19
5.2 B-150P-BRPL 20
6. Disimballaggio 21
7. Assemblaggio 21
7.1 B-150P-MRPL 21
7.2 B-150P-BRPL 22
7.3 Procedura di assemblaggio 23
8. Uso del microscopio 24
8.1 Regolazione intensità luminosa 24
8.2 Regolazione della frizione 24
8.3 Tavolino 24
8.4 Regolazione distanza interpupillare 24
8.5 Regolazione diottrica 25
8.6 Diaframma di apertura 25
8.7 Uso con batterie ricaricabili 25
8.8 Uso in campo chiaro 26
8.9 Uso in luce polarizzata 26
9. Manutenzione 27
10. Risoluzione dei problemi 28
Smaltimento 29
Sommario

Pagina 18
1. Avvertenza
Questo microscopio è uno strumento scientico di alta precisione, progettato per durare a lungo con una minima
manutenzione; la realizzazione è secondo i migliori standard ottici e meccanici, per poter essere utilizzato
quotidianamente. Vi ricordiamo che questo manuale contiene informazioni importanti per la sicurezza e per la
manutenzione dello strumento, e deve quindi essere messo a disposizione di coloro che lo utilizzeranno.
Decliniamo ogni responsabilità derivante da un utilizzo dello strumento non indicato nel presente manuale.
2. Simboli
La seguente tabella riporta i simboli utilizzati in questo manuale.
PERICOLO
Questo simbolo indica un rischio potenziale ed avverte di procedere con cautela.
SHOCK ELETTRICO
Questo simbolo indica un rischio di shock elettrico.
3. Informazioni sulla sicurezza
Per evitare shock elettrici
Prima di collegare il cavo di alimentazione alla presa elettrica, assicurarsi che il voltaggio della rete locale
coincida con il voltaggio dello strumento e che l’interruttore dell’illuminazione sia nella posizione “OFF”.
Gli utenti dovranno seguire tutte le norme di sicurezza locali. Lo strumento è certicato CE. In ogni caso, gli
utilizzatori sono gli unici responsabili per un utilizzo sicuro dello strumento. Per l’utilizzo in sicurezza dello
strumento è importante attenersi alle seguenti istruzioni e leggere il manuale in tutte le sue parti.
4. Utilizzo previsto
Modelli standard
Solo per applicazioni di ricerca ed usi didattici. Non indicato per utilizzo diagnostico e terapeutico umano e ve-
terinario.
Modelli IVD
Anche per uso diagnostico, nalizzato ad ottenere informazioni sulla situazione siologica o patologica del sog-
getto.

Pagina 19
5. Descrizione dello strumento
MANOPOLA
MACROMETRICA DI
MESSA A FUOCO
MANOPOLA
MICROMETRICA DI
MESSA A FUOCO
OCULARE
TESTA DI
OSSERVAZIONE
INTERRUTTORE / ROTELLA
REGOLAZIONE INTENSITÀ
CONDENSATORE
TAVOLINO
LED INDICATORE DI
CARICA
REVOLVER
OBIETTIVI
ANELLO
REGOLAZIONE
TENSIONE
MOLLETTINE
FERMACAMPIONE
POLARIZZATORE
ANALIZZATORE
5.1 B-150P-MRPL

Pagina 20
5.2 B-150P-BRPL
MOLLETTINE
FERMACAMPIONE
LED
INDICATORE DI
CARICA
MANOPOLA
MICROMETRICA DI
MESSA A FUOCO
TAVOLINO
TESTA DI
OSSERVAZIONE
CONDENSATORE
OBIETTIVI
INTERRUTTORE / ROTELLA
REGOLAZIONE INTENSITÀ
REVOLVER
OCULARI
POLARIZZATORE
ANALIZZATORE
MANOPOLA
MACROMETRICA DI
MESSA A FUOCO
ANELLO
REGOLAZIONE
TENSIONE