Optika Italy B-510 Series User manual

Ver. 2.1 2019
INSTRUCTION MANUAL
B-510 Series
Model
B-510DK

Page 2
Table of Contents
1. Warning 3
2. Symbols and conventions 3
3. Safety Information 3
4. Intended use 3
5. Overview 4
6. Unpacking 6
7. Assembling 6
7.1 Assembling procedure 7
8. Summaryofbrighteldobservationprocedures 9
9. Summaryofdarkeldobservationprocedures 10
10. Use of the microscope 11
10.1 Light intensity adjustment 11
10.2 Coarse focus tension adjustment 11
10.3 Coarse upper limit knob 11
10.4 Stage 11
10.5 Dioptric adjustment 12
10.6 Adjusting the interpupillary distance 12
10.7 Use of eye shields 12
10.8 Centeringthebrighteldcondenser 13
10.9 Effectsoftheelddiaphragm 13
10.10 Aperture diaphragm 13
11. Darkeldmicroscopy 14
11.1 Principles of oil immersion microscopy 14
11.2 Principlesofdarkeldillumination 16
11.3 Highmagnicationdarkeldmicroscopy 17
11.4 Centeringofdarkeldcondenser 18
12. Microphotography 21
12.1 Use of C-mount camera 21
12.2 UseofReexcamera 21
13. Maintenance 22
14. Troubleshooting 23
Equipmentdisposal 25

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of
maintenance. It is built to high optical and mechanical standards and to withstand daily use. We remind you
that this manual contains important information on safety and maintenance, and that it must therefore be made
accessible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses
that does not comply with this manual.
2. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICALSHOCK
This symbol indicates a risk of electrical shock.
3. Safety Information
  AvoidingElectricalShock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the
operation voltage of the equipment and that the lamp switch is in off position. Users should observe all safety
regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibility
to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure
safe operation of the unit.
4. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the
subject.

Page 4
5. Overview
PHOTO/TV
PORT
DIOPTER
ADJUSTMENT
RING
NOSEPIECE
COARSE
UPPER
LIMIT KNOB
OBJECTIVE
FIELD DIAPHRAGM
MAIN SWITCH /
BRIGHTNESS ADJUSTMENT KNOB
OBSERVATION
HEAD
CONDENSER HEIGHT
ADJUSTMENT KNOB

Page 5
EYEPIECES
STAGE
CONDENSER
BINOCULAR
TUBE
X/Y MOVEMENT
KNOBS
CONDENSER
CENTERING SCREWS
TENSION ADJUSTMENT RING
SLIDE HOLDER
FINE FOCUS
KNOB
COARSE FOCUS KNOB
Overview (opposite side)
LED CONDENSER
CENTERING SCREWS
DARK FIELD
CONDENSER
CONNECTOR

Page 6
6. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container
and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)
fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope
from the container and put it on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or
other residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
7. Assembling
Once opened the B-510DK box, the microscope parts are the following:
① Microscope frame
② Eyepieces
③ Objectives
④ Observation head
⑤ Immersion oil
⑥ Allen wrench
⑦ Tension adjustment tool
⑧ Dust cover
⑨ Power supply
⑩ Bright eld condenser
⑪ Dark eld condenser
⑫ Centering telescope
③
④
⑤
⑥
⑦
⑧⑨
①
②
⑩
⑪
⑫

Page 7
7.1 Assembling procedure
1. Insert the optical head above the stand and
tighten the screw. (Fig. 1)
• Hold the head with one hand during the
locking in order to avoid that the head falls.
2. Insert both eyepieces into the tubes of the optical
head. (Fig. 2)
3. Screw each objective into the thread of
the nosepiece, clockwise with increasing
magnication. (Fig. 3)
4. Insert the power supply jack in the connector
placed at the rear side of the microscope. (Fig. 4)
Fig. 1
Fig. 2
Fig. 3
Fig. 4

Page 8
• The microscope is delivered with two
condensers: one for brighteld and one for
darkeld. Select the suitable condenser for
the observation mode desired.
5. Lower the condenser holder using the height
condenser knob ①. (Fig. 5)
6. Insert the condenser round dovetail in the
condenser holder. (Fig. 6)
7. Tight the condenser locking screw ②. (Fig. 7)
8. (Only for darkeld condenser)
Connect the condenser jack to the connector on
the right side of the frame. (Fig. 8)
• When the condenser plug is connected, the
lightcomingfrom themicroscopeLED goes
outandtheinternalcondenserLEDturnson.
Fig. 5
Fig. 6
Fig. 7
Fig. 8
DF
BF
②

Page 9
8. Summaryofbrighteldobservationprocedures
Main switch /
brightness adjustment knob
(Used commands) (Chapter)
Slide holder
X/Y movement knobs
Nosepiece
Binocular tube
Diopter adjustment ring
Condenser height adjustment knob
Condenser centering screws
Aperture diaphragm
Field diaphragm
Nosepiece
Main switch /
brightness adjustment knob
Coarse and fine focus knobs
Coarse and fine focus knobs
Set the main swith to “ON” and adjust light intensity..
Place a slide on the stage.
Insert 10x objective into the light path
Focus the specimen
Begin observation
Adjust aperture and field diaphragms
Insert in the light path the desired objective and focus the
specimen
Adjust interpupillary distance
Adjust diopter
Center optical axis
Adjust brightness
(5/10)
(10)
(5)
(5)
(5)
(5)
(5)
(10)
(10)
(10)
(10)
(10)
(10)
(5/10)

Page 10
9. Summaryofdarkeldobservationprocedures
Main switch /
brightness adjustment knob
(Used commands) (Chapter)
Slide holder
X/Y movement knobs
Nosepiece
Binocular tube
Diopter adjustment ring
Condenser height adjustment knob
Condenser centering screws
Condenser height adjustment knob
Nosepiece
Main switch /
brightness adjustment knob
Coarse and fine focus knobs
Coarse and fine focus knobs
Set the main swith to “ON” and adjust light intensity.
Place a slide on the stage.
Insert 10x objective into the light path
Focus the specimen
Begin observation
Lower the condenser, place a drop of oil on the front lens
and return the condenser to the original height
Insert in the light path the 100x objective and focus the
specimen
Adjust interpupillary distance
Adjust diopter
Center optical axis
Adjust brightness
(5/10)
(10)
(5)
(5)
(5)
(5)
(5)
(10)
(10)
(10)
(10)
(10)
(5/10)
Nosepiece
Rotate the nosepiece to an intermediate position between
the 40x and 100x objectives and place a drop of oil on the
slide (5)
100x OIL/IRIS objective
Turn the iris diaphragm on the 100x objective to achieve the
darkfield effect (11)
Main switch /
brightness adjustment knob
Adjust brightness (5/10)