Optika B-1000 POL User manual

Version: 1
Issued: 25, 02, 2015
Model
B-1000POL
B-1000 Series
INSTRUCTION MANUAL

Page 2
Table of Contents
Warning
Symbols and conventions
Safety Information
Intended use
List of accessories and spare parts
Overview
Unpacking
Assembling
Using the microscope
Maintenance
Troubleshooting
Equipment disposal

Page 3
Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of mainte-
nance. It is built to high optical and mechanical standards and to withstand daily use. We remind you that this
manual contains important information on safety and maintenance, and that it must therefore be made acces-
sible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses that does
not comply with this manual.
Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the
operation voltage of the equipment and that the lamp switch is in off position. Users should observe all safety
regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibil-
ity to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure
safe operation of the unit.
Intended use
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.

Page 4
List of accessories and spare parts
CAT. NO. DESCRIPTION
M-1001 WF10x/22mm eyepiece (pair)
M-1002 WF10x/24mm eyepiece (pair)
M-1004.N Centering telescope, 30mm diameter
M-781 WF10x/22mm micrometer eyepiece (10mm, 0.1mm div.)
M-1011 Trinocular Head (3 positions)
M-1012 Binocular ERGO head
M-1033 Bertrand Lens with Analyzer and slot for slides (with Lambda, 1/4 Lambda and Quartz Edge)
M-1044 Quintuple nosepiece with centrable positions for POL objectives
M-1080 4x IOS POL PLAN objective
M-1081 10x IOS POL PLAN objective
M-1082 40x IOS POL PLAN objective
M-1083 60x IOS POL PLAN objective
M-1145 Rotating Stage, centrable
M-1146 Attachable mechanical stage for rotating Stage
M-005 Micrometric slide, 26x76mm, range 1mm, div. 0,01mm
M-690 Eyecup (pair)
M-619 Photo tube adapter for full frame SLR camera
M-173 Photo tube adapter for APS-C SLR camera
M-699 Photo tube adapter for DIGI digital camera series
M-620 CCD camera adapter for 1/3’’ sensor
M-620.1 CCD camera adapter for ½’’ sensor
M-114 CCD camera adapter 0,45x
M-113.1 Ring adapter, 30mm (for monocular and binocular microscopes)
M-617.1N Phase contrast set with IOS PLAN objectives 40x

Page 5
Overview
STAGE LOCK
KNOB
COARSE
FOCUSING
KNOB
NOSEPIECE
DIOPTRIC
ADJUSTMENT
RING
PHOTO PORT
MAIN ON/OFF
EYEPIECE
ANALYZER
FILTER
BERTRAND LENS
DISC
CONDENSER
CENTERING
KNOBS
APERTURE
DIAPHRAGM
POLARIZER
FIELD DIAPHRAGM
BRIGHTNESS
ADJUSTMENT KNOB
FOCUS-STOP KNOB
FINE FOCUSING
KNOB
OBJECTIVE
STAGE
FILTER
PLATE HOLDER

Page 6
Put the microscope stand on a
solid table.
First insert the Bertrand lens
attachment, use the 2mm
Allen wrench to tighten the
screw.
All screws are already inser-
ted into each threaded hole.
(Fig.1)
Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container
and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)
fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope
from the container and put it on a stable desk.
Assembling
Once you open the box, these are the microscope’s components:
① Condenser
② Optical head
③ Main body
④ Eyepieces
⑤ Power supply
⑥ Stage
⑦ Objectives
⑧ Bertrand lens and analyzer
⑨ Retardation plates
①
②
③
④
⑤
⑥
⑦
⑧ ⑨
Fig.1

Page 7
Insert the condenser under
the stage: position until it is
well inserted into its holder
(under the condenser there is
a pin that must fully enter the
guide of the holder). (Fig. 4)
AFTER INSERTING THE
CONDENSER, TIGHTEN
THIS SCREW
Insert the optical head above
the Bertrand lens, using the
other 3mm Allen wrench to
tighten the screw. (Fig.2)
Insert both eyepieces into
the tubes of the optical head.
(Fig.3)
SPRING
Mount the rotating stage: at
the bottom of the stage there
is a spring, push this spring
toward the stage support ①,
then push the stage
downward ②. (Fig.5)
Fig.2
Fig.3
Fig.4
Fig.5
①
②

Page 8
Insert the retardation plates
by sliding them into the slot
under the optical head. (Fig.7)
Center each objective using
the provided screwdrivers
(see chapter using the micro-
scope). (Fig.8)
Insert the power supply jack
on the connector at the rear.
(Fig.9)
Screw each objective into the
thread of the nosepiece, in
order of magnication. (Fig.6)
Fig.6
Fig.7
Fig.8
Fig.9

Page 9
The rotating stage is provided
with:
a) Stage centering knobs;
b) Stage lock knob;
c) 45° rotation clicks;
d) Optional X-Y translator.
(Fig.10)
The Bertrand lens attachment
is provided with
a) Analyzer lter;
b) Retardation plates;
c) Lens focus adjustment;
d) Lens X-Y centering screws.
(Fig.11)
X-Y TRANSLATION KNOBS
STAGE CENTERING KNOBS
BERTRAND LENS ATTACHMENT
RETARDATION PLATES
Fig.10
Fig.11

Page 10
B-1000 illumination can be managed through
the keyboard placed on the left of the stand:
1) ON-OFF: press this button to turn on or off
the LED illuminator.
2) BOOST: press this button in order to incre-
ase the brightness (useful for high-magnica-
tion objectives or very opaque specimens).
2) AUTO-OFF: if you want the illuminator to
switch off automatically, press this button until
15, 30 or 60 minutes delay is set. After this
period of time, the light will turn off. You have
to press the ON-OFF button to turn it on again.
(Fig.12)
In order to activate the transmitted light illu-
minator, insert the plug of the external power
supply into the socket and turn on the main
switch on the side of the main body. Press
the ON-OFF button on the control keyboard
and turn the brightness adjustment knob to a
brightness suitable for observation. (Fig.13)
Do not enable boost mode while observing with
low magnication objectives (4x, 10x) with fully
open diaphgram: the high brightness may hurt
user’s eyes.
Control keyboard
LED settings
Preliminary settings
1) ON-OFF
3) AUTO-OFF
2) BOOST
MAIN
SWITCH
• The eld diaphragm is
fully open. (Fig.14)
• The aperture diaphragm
is fully open. (Fig.15)
• No lter is inserted under
the observation head.
Using the microscope
Fig.12
Fig.13
Fig.14
Fig.15
Before trying to focus the
sample, ensure that: