REPLIGEN CTech FlowVPE Operating manual

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CTech™ FlowVPE® System Maintenance Guide
Introducon
The purpose of this document is to become familiar and comfortable with handling the various components of the
GxP Flow Cell as well as correctly following the loading and unloading procedures. The following instrucon details
these procedures, as well as the setup required for the FlowVPE hardware prior to the loading procedure.
How to Load the GxP Flow Cell and Fibree
1. Click on Load Controls in the FlowVPE sidebar. The Flow Head moves to the loading posion and opens the Load
Controls window.
Figure 1. Load Controls buon.
CTech™ FlowVPE 10 mm GxP Flow Cell Loading Procedure
DOC0093 Rev. 02 11/12/2021
WARNING
! !
These instrucons must be followed very closely, as they ensure proper loading and operaon of the
FlowVPE System. Failure to do so may result in damage to the Flow hardware.
The User is the Crical Link Between the Soware and Hardware:
The user must tell the soware when the Flow Cell is mounted and demounted. The
soware is set up to prompt accordingly, but the user must be aware of the current state
of the hardware for safe and eecve use of the FlowVPE System.

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CTech™ FlowVPE® System Maintenance Guide
2. Make sure the Flow Cell is selected in the dropdown menu. Select FC-GxP10-SS for the 10 mm GxP Flow Cell.
Figure 2. Select Flow Cell from Cell Load/Unload Controls window.
3. Input the serial number, which is located on the side of the Flow Cell. Make sure to type the serial number
without the hyphen.
Figure 3. Serial Number prompt.
4. Line up the dovetail on the Flow Cell with the dovetail on the Detector Module, and gently slide the Flow Cell
into place (see Figure 4). DO NOT FORCE THE CELL IF IT DOES NOT SLIDE EASILY. Remove the Flow Cell and try
again, ensuring both sides of the Flow Cell are engaged with the Detector Module. For assistance in mounng
the Flow Cell properly, refer to General Troubleshoong in the FlowVPE User Manual DOC0096.
DO NOT CLICK LOAD UNTIL YOU HAVE MOUNTED THE
FLOW CELL AND FIBRETTE.
! !

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CTech™ FlowVPE® System Maintenance Guide
Figure 4. Flow Fibree Flange ng into Fibree clamp.
5. Slide the Flow Cell and Flow Fibree into place. The Flow Cell will make a hard stop on the pin at the back of the
Detector Module. The Fibree will slide into the Fibree Clamp.
Figure 5. Flow Cell mounng to Flow Head.
6. Secure the Flow Cell into the Detector Module using the four capve fasteners.
Figure 6. Securing Flow Cell to Detector Module.

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CTech™ FlowVPE® System Maintenance Guide
7. Push up on the Handle Lock on top of the Fibree Clamp to move it out of the way of the Clamp Handle, as
shown in Figure 7 (le).
8. Pull the Clamp Handle down, towards yourself, to close the Fibree Clamp over the Fibree Flange, as shown in
Figure 7 (right).
9. Apply pressure unl the Handle Lock falls into place behind the Handle.
Figure 7. Closing Fibree Clamp over Flow Fibree.
10. Swing the Clamp Lock Arm all the way to the right and seat the thumb nut into the mang counter bore.
Figure 8. Locking Fibree Clamp posion.
11. Tighten the thumb nut into the mang counter bore unl it makes a hard stop.

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CTech™ FlowVPE® System Maintenance Guide
12. Connect the SC-ST adapter to the Flow Fibree by aligning the spline of the Fibree with the channel of the
adapter. Gently insert the adapter fully into the locking collar of the Fibree. Li up and twist the collar to
secure the pegs on either side, as shown in Figure 9.
Figure 9. Securing SC-ST adapter to Flow Fibree.
13. Insert the tan poron of the Delivery Fiber into the SC-ST adapter so the small spline on the tan Delivery Fiber
connecter lines up with the channel in the adapter.
Figure 10. Delivery Fiber connected to Flow Fibree.

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CTech™ FlowVPE® System Maintenance Guide
14. Close the cover door. When the Flow Cell and Flow Fibree have been mounted onto the Flow Head properly,
the FlowVPE instrument will appear as shown in Figure 11. Connue the steps to nalize the setup process.
Figure 11. FlowVPE instrument with mounted Flow Cell.

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CTech™ FlowVPE® System Maintenance Guide
15. Click Load to tell the program that the Flow Cell and Fibree are mounted into the Flow Head properly.
Figure 12. UNLOADED Status in Cell Load/Unload Controls window.
16. The Load Status in the center of the Load/Unload Controls box will turn blue and will show the Load Status as
LOADED to conrm that the Flow Cell is loaded.
Figure 13. LOADED Status in Cell Load/Unload Controls window.
Congratulaons, you have now successfully loaded the Flow Cell!

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CTech™ FlowVPE® System Maintenance Guide
How to Unload the GxP Flow Cell and Fibree
1. Select Load Controls from the sidebar in the FlowVPE soware (Figure 1).
2. Open the FlowVPE instrument cover.
3. Disconnect the Delivery Fiber from the Flow Fibree.
4. Loosen and open the Clamp Lock Arm by turning the thumb nut counterclockwise.
5. Push back on the Handle Lock.
6. Push up on the Clamp Handle to open the clamp.
7. Loosen all four capve fasteners on the Flow Cell Body to remove the Flow Cell from the Detector Module.
8. Gently slide the Flow Cell out of the Fibree Clamp.
9. In the FlowVPE soware, click on Unload in the Load/Unload Controls window.
10. Follow prompts in the soware to fully unload the Flow Cell.
11. The Load Status in the Load/Unload Controls window should now read UNLOADED.