REPLIGEN KrosFlo KR2i User manual

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© 2023 Repligen Corporation. All rights reserved. The trademarks mentioned herein
are the property of Repligen Corporation and/or its affiliate(s) or their respective owners.
DOC0304 eRev. [Major Version]1.01.0 [Publication Date]8/9/20238/9/2023
KrosFlo® KR2i
RPM™ System
User Guide

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The information contained in this document is subject to change without notice.
With respect to documentation accompanying Product Repligen makes no warranty, express or implied. Any and all warranties
related to the documentation accompanying Product are expressly disclaimed. Customer shall refer to the terms and conditions of
sale governing the transaction for any and all warranties for the Product.
Repligen Corporation shall not be liable for errors contained herein or for incidental or consequential damages in connection with
the furnishing, performance, or use of this material.
No part of this document may be photocopied, reproduced, or translated to another language without the prior written consent of
Repligen Corporation.
Products are not intended for diagnostic or therapeutic use or for use in vivo with humans or animals.
For further information, please contact Repligen Corporation at www.repligen.com.
©2023 Repligen Corporation. All rights reserved. The trademarks mentioned herein are the property of Repligen Corporation and/or
its affiliate(s) or their respective owners.
Customer Support
[e-mail]
[e-mail]
[phone]
Repligen Corporation
Process Analytics Offices and Manufacturing Facility
685 Route 202/206
Bridgewater, NJ 08807, USA
www.repligen.com

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Contents
9.1.1 Connecting the FlowVPX Head to the FlowVPX Standard Mount.................................................................................. 26
9.1.2 Connecting the FlowVPX Instrument to the Cary 60 Spectrophotometer and Computer............................................. 29
9.2.1 Loading the Flow Cell ..................................................................................................................................................... 33
9.2.2 Unloading the Flow Cell ................................................................................................................................................. 35
10.1.1 Installation...................................................................................................................................................................... 37
10.2.1 Installation...................................................................................................................................................................... 37
10.2.2 Auxiliary Pump Settings.................................................................................................................................................. 38
10.3.1 ABV Installation .............................................................................................................................................................. 38
10.3.2 ABV Settings ................................................................................................................................................................... 38
10.4.1 System Configuration ..................................................................................................................................................... 39
10.4.2 Basic Setup ..................................................................................................................................................................... 39
10.4.3 Maintenance .................................................................................................................................................................. 40

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List of tables
Table 1. Explanation of user attention phrases ......................................................................................................................................... 7
Table 2. Safety precautions for KrosFlo KR2i RPM System ........................................................................................................................ 8
Table 3. Performance Specifications.......................................................................................................................................................... 9
Table 4. Electrical Input Specifications ......................................................................................................................................................9
Table 5. Physical Specifications................................................................................................................................................................10
Table 6. Environmental Specifications .....................................................................................................................................................11
Table 7. Compliance Summary ................................................................................................................................................................ 11
Table 8. Computer Specifications: Included Tablet .................................................................................................................................12
Table 9. Computer Specifications: Minimum Requirements (If Supplied by User).................................................................................. 12
Table 10. System Parts List ......................................................................................................................................................................13
Table 11. Materials of Construction.........................................................................................................................................................14
Table 12. ACR2-H3I-01N Typical Flow, Pressure, and Vacuum Data – 3 roller pumps ............................................................................21
Table 13. ACR2-H4I-01N Typical Flow, Pressure, and Vacuum Data – 3 roller pumps ............................................................................21
Table 14. ACR2-H4I-01N Typical Flow, Pressure, and Vacuum Data – 3 roller pumps ............................................................................32
Table 15. KR Jr Tubing Specifications....................................................................................................................................................... 38
Table 16. Troubleshooting .......................................................................................................................................................................43
Table 17. Replacement and Auxiliary Parts Information .........................................................................................................................44
List of figures
Figure 1. Manual Mode setup configuration ...........................................................................................................................................15
Figure 2. Concentration Mode (C. Mode) setup configuration................................................................................................................16
Figure 3. C/D and C/D/C Mode setup configuration................................................................................................................................17
Figure 4. C/D/D/C Mode setup configuration..........................................................................................................................................17
Figure 5. CFC Mode setup configuration ................................................................................................................................................. 18
Figure 6. CF/D/C Mode setup configuration ............................................................................................................................................19
Figure 7. Attaching mounting plate to drive............................................................................................................................................22
Figure 8. Back of Research II Pump Head to drive ...................................................................................................................................22
Figure 9. Position for engaging bayonet feature for horizontal mounting ..............................................................................................22
Figure 10. Bayonet feature locked in horizontal pump orientation ........................................................................................................23
Figure 11. Pump head in fully closed position .........................................................................................................................................23
Figure 12. Pump head in fully open position ........................................................................................................................................... 24
Figure 13. Tubing path through pump head—during loading .................................................................................................................24
Figure 14. Preparation to mount a second pump head...........................................................................................................................25
Figure 15. Engaging bayonet of second pump head to bayonet tabs on first pump head......................................................................25
Figure 16. Drive with both pump heads locked in position first pump head...........................................................................................25
Figure 17. CTech FlowVPX System: Body .................................................................................................................................................26
Figure 18. FlowVPX Head, logo side down............................................................................................................................................... 26
Figure 19. Standard Mount Clamp on FlowVPX Head .............................................................................................................................27
Figure 20. 5 mm Ball End Driver...............................................................................................................................................................27
Figure 21. Clamp Handle installation .......................................................................................................................................................27

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Figure 22. Rotate Clamp Handle ..............................................................................................................................................................27
Figure 23. Tighten Mounting Post Clamp Handle .................................................................................................................................... 28
Figure 24. Install FlowVPX Head on mounting post.................................................................................................................................28
Figure 25. Lower FlowVPX Head on mounting post................................................................................................................................. 28
Figure 26. Tighten Standard Mount Clamp Handle (Left) and Repositioning the Clamp Handle (Right).................................................29
Figure 28. Accessory port in back of Cary 60 spectrophotometer...........................................................................................................29
Figure 29. Detector Cable in sample compartment................................................................................................................................. 30
Figure 30. Cary 60 power cable and USB cable, back panel .................................................................................................................... 30
Figure 31. Fiber Optic Coupler threaded splice bushing..........................................................................................................................30
Figure 32. FlowVPX I/O cable connections ..............................................................................................................................................31
Figure 33. Connect Power and I/O Splitter Cable (EC0208) to FlowVPX Head ........................................................................................31
Figure 34. I/O External Cable (EC0214) to FlowVPX Head .......................................................................................................................32
Figure 35. USB Cable (EC0207) to FlowVPX Head .................................................................................................................................... 32
Figure 36. FlowVPX USB cable (EC0207) to computer .............................................................................................................................33
Figure 36. KR Jr Display in Remote Control Mode ...................................................................................................................................37
Figure 37. Automatic Backpressure Valve ............................................................................................................................................... 38
Figure 38. Back of KONDUiT, green power indicator light .......................................................................................................................39
Figure 39. Front of KONDUiT ...................................................................................................................................................................40
Abbreviations
CF Concentration factor
CFC Constant feed concentration
CV Column volume
HF Hollow fiber
KF KrosFlo®
KR2i KrosFlo Research 2 integrated
PPE Personal protective equipment
RPM Real-Time Process Management
TMP Transmembrane pressure
TFF Tangential flow filtration
VPT Variable Pathlength Technology

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1. Introduction
This user guide provides detailed instructions for the set up and operation of the KR2i RPM Tangential Flow Filtration (TFF) system
and auxiliary components. Included are descriptions of potential modes of operation and basic concepts of tangential flow filtration.
For questions and further information, please contact your Repligen representative.
The KrosFlo® KR2i RPM™ System is the first Tangential Flow Filtration (TFF) bench-scale system with integrated Real-time Process
Management (RPM). The System combines the KrosFlo® KR2i TFF System and the CTech™ FlowVPX® In-line spectrophotometer for
real-time concentration measurement and control. The KrosFlo® RPM™ software can execute complicated TFF processes through
user-specified set points for the system auxiliary pumps, scales, and backpressure valve.
The system is compatible with both hollow fiber (HF) membrane and TangenX flat sheet cassette TFF modules. The HF modules are
ideal for sample concentration, fractionation, and washing while avoiding membrane fouling and maximizing product recovery.
TangenX cassettes feature an inner screen that increases turbulence and gives high permeate flux, ideal for applications involving
viscous proteins, oligosaccharides, and viruses. Both module types are disposable to eliminate the potential for cross-contamination.
This user guide provides guidance for general use of the KrosFlo KR2i RPM System. For further optimization or troubleshooting
support, please contact our Customer Service team:
[e-mail]
[e-mail]
[phone]

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2. About this document
This manual uses several different phrases. Each phrase should draw the following level of attention:
Table 1. Explanation of user attention phrases
Phrase Description
Note: Points out useful information.
IMPORTANT Indicates information necessary for proper instrument operation.
PRECAUTION Cautions users of potential physical injury or equipment damage if the information is not heeded.
WARNING! Warns users that serious physical injury can result if warning precautions are not heeded.

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3. Safety precautions
Table 2. Safety precautions for KrosFlo KR2i RPM System
Symbol Description
DANGER
High voltages exist and are accessible. Use extreme caution when servicing internal components.
Remove power from the pump before any cleaning operation is started.
WARNING Remove power from the pump before attempting any maintenance.
WARNINGS
•Tubing breakage may result in fluid being sprayed from pump. Use appropriate measures to protect
operator and equipment.
•Turn drive off before removing or installing tubes. Fingers or loose clothing could get caught in
drive mechanism.
CAUTIONS
•Power must be turned off before connecting the external remote control cable to prevent damage
to the drive.
•Do not contaminate the lubricant in the container, on the shaft or on the seal with foreign material.
Failure to observe this precaution may result in damage to the seal and premature failure of the
seal.
•No foreign matter should be allowed under the gasket on the back of the front plate or under the
heads of the screws. Failure to observe this precaution may result in leakage during washdown of
the drive.
•To avoid electrical shock, the power cord protective grounding conductor must be connected to
ground. Not for operation in wet locations as defined by EN61010-1.
CAUTION Keep fingers away from rotor while pump is in operation. Stop pump before loading or unloading tubing.
CAUTION
Caution, indicates a hazard that may result in personal injury or death if proper operating procedures are not
followed. Documentation must be consulted in all cases where this symbol is marked. Do not proceed
beyond a [Caution] notice until procedures and conditions of operation are met as specified.
CAUTION Caution, possibility of shock

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4. System Specifications
The tables below outline the performance specifications, electrical requirements, physical attributes, environmental considerations,
and compliance information of the major system components. Tubing specifications can be found in section 8.2.
Table 3. Performance Specifications
Table 4. Electrical Input Specifications
Specification Value
KR2i TFF System
Pump Speed Range 0.1 to 600 rpm
Maximum torque load—Starting 400 oz-in (29 kg-cm)
Maximum torque load—Running Up to 180 oz-in (13 kg-cm)
Speed regulation
Line ±0.1% FS
Load ±0.1% FS
Drift ±0.1% FS
Display 128 x 64 LCD w/ LED Backlight
Pressure Sensor Limits -9.99 to 75 psi
FlowVPX System
Qualification Slope Range 0.10 to 46 Au/mm using NIST-Traceable Slope Standards
Qualification Slope Repeatability ±2%
Maximum Pathlength 5.000 mm
Minimum Pathlength Step 0.001 mm
Spectroscopic Engine Agilent Cary 60 Spectrophotometer
Spectrophotometer Wavelength Range 190–1100 nm
KONDUiT Conductivity, Temperature, and UV Monitor
Conductivity Range 0.1 to 100 mS/cm
Conductivity Accuracy
0.1 to 2 mS/cm: ± 0.1 mS/cm
2 to 50 mS/cm: ±5% of reading
50 to 100 mS/cm: ±5% of reading (typical)
Temperature Range 0–70°C
Temperature Accuracy Better than ±0.2°C (typically better than ±0.1°C)
UV Sensor Output signal
4–20 mA sourcing with 400 Ωmaximum at 24 VDC; scaled to 0–2 AU with
repeatability of 1% of full scale (0.02 AU)
UV Sensor Typical Response Time 1 second
UV Sensor Maximum Zero Shift <2% of full scale (<0.040 AU)
UV Sensor Long-term Output Drift <5% per month of full scale (<0.100 AU)
Specification Value

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Table 5. Physical Specifications
Specification Value
KR2i Main Pump
Dimensions (L × W × H)
10.5 in × 8 in × 8 in
(267 × 203 × 203 mm)
Weight 13 lb. (5.9 kg)
KR2i Pump Heads
Mounted dimensions (W x H x D) 8.8 cm x 12.1 cm x 7.8 cm (3.45" x 4.75" x 3.08")
Weight 1.1 lb (0.5 kg)
Housing Materials Glass-filled polypropylene (PP), polyphenylene sulfide (PPS), nylon (PA)
Roller Material Stainless Steel
Bearing Material Sealed Stainless Steel
Rotor material Stainless Steel
FlowVPX Instrument
Dimensions (L × W × H)
4” x 4¾” x 9”
(102 x 121 x 229 mm)
Weight
4.31 kg (with 3 mm Flow Cell)
4.22 kg (with 10 mm Flow Cell)
5.54 kg (with 22 mm Flow Cell)
Delivery Fiber Optic Cable Length 3 m (Optional 6 m Fiber Available Upon Request)
Cary 60 Spectrophotometer
Dimensions
22" x 19" x 8"
(559 x 483 x 203 mm)
Weight 18.14 kg
KONDUiT Conductivity, Temperature, and UV Monitor
Dimensions (L × W × H) 7 ¾” x 4 ¾” x 4½”
KR2i TFF System
Supply voltage limits 90–260 VRMS @ 50–60 Hz (Universal Input)
Current, Maximum 2.2 A @ 115 VRMS, or 1.1 A @ 230 VRMS
FlowVPX System
Supply voltage limits 100–230 VAC @ 50–60 Hz
Current, Maximum 0.6 A
Cary 60 Spectrophotometer
Voltage 100–240 VAC
Input Frequency 47–63 Hz
KONDUiT Conductivity, Temperature, and UV Monitor
Voltage 24 VDC
Current 0.625 A

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Weight 1.6 kg
Housing Materials Powder-coated Aluminum, Urethane
Table 6. Environmental Specifications
‡ Use in this temperature range for continuous duty operation with no decrease in performance or product life. Pump Heads will work outside this
range with some possible reductions in performance or product life.
Table 7. Compliance Summary
Specification Value
KR2i Main Pump
Enclosure Rating IP33
Operating Temperature 0° to 40°C (32° to 104°F)
Storage Temperature –25° to 65°C (–13° to 149°F)
Humidity (non-condensing) 10% to 90%
Altitude Less than 2000 m
Pollution Degree Pollution Degree 2
KR2i Pump Heads
Chemical resistance Most substances, except strong acids or alkalis, organic solvents, or hydrocarbons
Operating Temperature ‡ 0°C to 40°C (32°F to 104°F)
Storage Temperature -45°C to 65°C (-49°F to 149°F)
Humidity 10% to 90% (non-condensing)
Altitude 2000 m or less
FlowVPX System
Enclosure Rating IP65
Operating Temperature 0°C to 48°C (32°F to 118°F)
Operating Humidity (non-condensing) 15% to 80%
Storage Temperature -34°C to 66°C (-29°F to 150°F)
Storage Humidity (non-condensing) 0% to 95%
Pollution Degree Pollution Degree 2
KONDUiT Conductivity, Temperature, and UV Monitor
Operating Temperature 2°C to 50°C (35°F to 122°F)
Storage Temperature -25°C to 65°C (-13°F to 149°F)
Pressure Rated for up to 75 psi (5 bar)
Specification Value
KR2i TFF System
ETL UL 61010-1, CAN/CSA C22.2 No. 61010-1

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4.1 Computer
A computer tablet is provided to control the KR2i RPM system. The user may supply their own computer, if preferred.
Table 8. Computer Specifications: Included Tablet
Table 9. Computer Specifications: Minimum Requirements (If Supplied by User)
CE
EN61010-1 (EU Low Voltage Directive)
EN61326 (EU EMC Directive)
RoHS Directive 2011/65/EU
FlowVPX System (including Agilent Cary 60)
ETL UL 61010-1, CAN/CSA C22.2 No. 61010-1
CE
EN61010-1 (EU Low Voltage Directive)
EN61326 (EU EMC Directive)
RoHS Directive 2011/65/EU
KONDUiT Conductivity, Temperature, and UV Monitor
ETL
Conforms to ANSI/UL Std 61010-1
Certified to CAN/CSA Std C22.2 No. 61010-1 This product has been tested to the
requirements of CAN/CSA-C22.2 No. 61010-1 second edition, including Amendment 1, or
a later version of the same standard incorporating the same level of testing requirements.
CE EN61010-1: (EU Low Voltage Directive)
EN61326: (EU EMC Directive)
Specification Value
Model Microsoft® Surface Pro®
Dimensions 11.5" x 7.9" x 0.33"
(292 x 201 x 8.5 mm)
Specification Value
Operating System Windows® 10
Processor i7
Hard Drive 250 GB (SSD Preferred)
RAM 16 GB

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5. System Configuration and Major Components
5.1 System Parts List
Table 10. System Parts List
For replacement part numbers, please see Section 14.
5.2 Pump Drive & Integrated Pressure Monitor
The KR2i TFF system comes with the Digital Pressure Monitor integrated into the Pump Drive as one unit. The internal
microprocessor runs both the Pump and Pressure monitor functions.
5.1 Octopus Cables
The KR2i TFF system comes with two Octopus Cables: one for the Auxiliary Components, and one for the Pressure Transducers. On
the back of the TFF system are two Interface ports for the Octopus Cables.
5.2 Mounting Hardware
The KR2i TFF system comes with an attached mounting plate for the Easy-Load Pump Head.
Part Description Quantity
KR2i TFF System
KrosFlo® Research 2i Pump Drive w/ Integrated Pressure Monitor 1
Microsoft® Surface Pro® with Installed KrosFlo RPM™ Software Suite 1
Auxiliary Component Octopus Cable 1
Pressure Transducer Cable 1
Power Supply Cable 1
KrosFlo® Research II Easy-Load Pump Head 1
KrosFlo 20 kg Scales 2
KR Jr Pump Head with Cables 2
KONDUiT 1
Magnetic Stirrer 1
FlowVPX System
FlowVPX Instrument Essentials Kit 1
FlowVPX Accessory Kit 1
FlowVPX Head Assembly 1
FlowVPX Detector Module 1
Agilent Cary 60 Spectrophotometer 1
Flow Cell (Choice of 3 mm or 10 mm Flow Path Diameter) 2

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6. Materials of Construction
Table 11. Materials of Construction
Part Number Material
KR2i TFF System Process Contact Surfaces
Tubing / Reservoir Closures C-Flex® / Pharmapure®
Reservoirs Polypropylene
Disposable Pressure Transducers Polysulfone
Plastic Fittings Polypropylene / Polysulfone
FlowVPX Flow Cell Process Contact Surfaces
Flow Path 316L Stainless Steel
Diaphragm Seal EPDM
Detector Window UV-Grade Fused Silica
Adhesive Medical-Grade Epoxy
KR2i TFF System Non-Process-Contact Surfaces
316 Stainless Steel
Hard Coat Anodized Aluminum
Polysulfone
Polypropylene
Polyphenylene Sulfide
Polyester
Nylon
FlowVPX System Non-Process-Contact Surfaces
316/316L Stainless Steel
Stainless Steel Fiber Optic Connector
Polyetheretherketone (PEEK)
Polyphenylsulfone (PPSU)
Medical-Grade Epoxy
Gold-Plated Electrical Contacts

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7. Setup and Operation
7.1 Basic Setup
Note: See Sections 7.2 through 7.7 for Sample Applications.
1. Mount the TFF system on a flat, horizontal surface with no more than two Pump Heads attached (see section 8.3 for Pump
Head Setup details).
2. Connect both Octopus Cables to the back of the TFF system.
3. Connect up to three pressure transducers to the Pressure Transducer Octopus Cable ports, depending on the application.
4. Connect an Automatic Backpressure Valves (ABV's) to the Auxiliary Component Octopus Cable "Valve" (see section 10.3 for
ABV Setup details).
5. Connect the FlowVPX to process tubing in between the feed reservoir and the KR2i pump. (See Section 9 FlowVPX setup
details.)
6. Connect power cable to the TFF system.
7. Follow guidelines and diagrams in sections 7.2 through 7.7 to determine which Auxiliary Components are required to
operate specific Process Modes for manual, semi-automated, and automated processes.
8. If using Auxiliary Pump, configure Auxiliary Pump before starting application (see section 10.2 for Auxiliary Pump Setup
details).
9. After connecting Auxiliary Components, power on the TFF system first before powering on Auxiliary Components.
10. Connect TFF flow path to TFF system.
11. Set low and high pressure alarms and interlocks as required by the process conditions.
12. Input Concentration Factor/Diafiltration Volume (CF/DV) set-points into the TFF system’s Process Mode settings to start
application.
Note: Valves, cables, and the computer are intentionally left out of the diagrams below for visual clarity.
7.2 Manual Mode Setup
Figure 1. Manual Mode setup configuration
VPT Device
(FlowVPX)
Feed Reservoir
Diafiltration Pump
(Auxiliary Pump 1)
Feed
Scale
KR2i Pump
Permeate Pump
(Auxiliary Pump 2)
Permeate Scale
Permeate Reservoir
Module
Diafiltration Buffer Reservoir

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1. Any combination of Auxiliary Components is possible in Manual Mode—the only required component is the TFF system
pump itself. Diagram above is a full setup with all Auxiliary Components.
2. Auxiliary Scales
a. Connect up to two scales to the Auxiliary Component Cable’s “Feed Scale” and/or “Permeate Scale” ports.
7.3 Concentration Mode (C. Mode) Setup
Figure 2. Concentration Mode (C. Mode) setup configuration.
1. Auxiliary Scales
a. Connect scale to the Auxiliary Component Cable’s “Permeate Scale”.
7.4 C/D and C/D/C Mode Setup
Note:Permeate Pump is optional and shown in the diagram as an example for applications that require permeate control.
Module
Permeate Reservoir
Feed Reservoir
KR2i Pump
VPT Device
(FlowVPX)
Permeate Scale

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Figure 3. C/D and C/D/C Mode setup configuration
1. Auxiliary Pumps
a. Connect auxiliary pump to the Auxiliary Component Cable’s “Auxiliary Pump 1” port and second auxiliary pump to
the Auxiliary Component Cable's "Auxiliary Pump 2" port.
b. Connect Auxiliary pump power cables(s).
2. Auxiliary Scales
a. Connect first scale to the Auxiliary Component Cable’s “Feed Scale” port and second scale to the Auxiliary
Component Cable’s “Permeate Scale” port.
7.5 C/D/D/C Mode Setup
Figure 4. C/D/D/C Mode setup configuration
1. Auxiliary Pumps
a. Connect first auxiliary pump to the Auxiliary Component Cable’s “Auxiliary Pump 1” port and second auxiliary
pump to the Auxiliary Component Cable’s “Auxiliary Pump 2” port.
b. Connect auxiliary power cables.
VPT Device
(FlowVPX)
Feed Reservoir
Diafiltration Pump
(Auxiliary Pump 1)
Feed
Scale
KR2i
Pump
Permeate Pump
(Auxiliary Pump 2)
Permeate
Scale
Permeate Reservoir
Module
Diafiltration Buffer Reservoir
Permeate Reservoir
VPT Device
(FlowVPX)
KR2i Pump
Diafiltration Pump
(Auxiliary Pump 2)
Permeate Scale
Diafiltration Buffer Reservoir 2
Diafiltration Buffer Reservoir 1
Diafiltration Pump
(Auxiliary Pump 1)
Feed Scale

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c. Auxiliary Pump 1 will function as Diafiltration Pump 1, and the Auxiliary Pump 2 will function as Diafiltration Pump
2.
2. Auxiliary Scales
a. Connect first scale to the Auxiliary Component Cable’s “Feed Scale” port and second scale to the Auxiliary
Component Cable’s “Permeate Scale” port.
7.6 CFC Mode Setup
Note:Permeate Pump is optional and shown in the diagram as an example for applications that require permeate control.
Figure 5. CFC Mode setup configuration
1. Auxiliary Scales
a. Connect first scale to the Auxiliary Component Cable’s “Feed Scale” port and second scale to the Auxiliary
Component Cable’s “Permeate Scale” port.
VPT Device
(FlowVPX)
Feed Reservoir
Diafiltration Pump
(Auxiliary Pump 1)
Feed
Scale
KR2i Pump
Permeate Pump
(Auxiliary Pump 2)
Permeate
Scale
Permeate Reservoir
Module
Diafiltration Buffer Reservoir

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7.7 CF/D/C Mode Setup
Figure 6. CF/D/C Mode setup configuration
7.8 System Setup Using KrosFlo RPM Software Pump Control Window
The Pump Control Interface can be used to interact with all the features of the system. This screen will appear when the KrosFlo
RPM software is opened. When needed, the functions and setpoints are accessible on the main pump display.
7.9 Alarms and Stops
The TFF system has a number of safety pressure alarms and stops that may be set in the Alarm Settings menu.
•Pf Hi Stop: When Feed Pressure (Pf) value ≥ Pf Hi Stop value, the pump drive will stop running.
•Pf Hi Alarm:When Pf value ≥ Pf Hi Alarm value, the pump drive will alarm but continue running.
•Pf Lo Stop: When Pf value ≤ Pf Lo Alarm value, the pump drive will stop running.
•Pf Lo Alarm: When Pf value ≤ Pf Lo Alarm value, the pump drive will alarm but continue running.
Note:For the Pf Lo Stop and Lo Alarm the feed pressure must first rise above the alarm level to be activated.
•Pp Lo Alarm:When Permeate Pressure (Pp) value ≤ Pp Lo Alarm value, the pump drive will alarm but continue running.
•Pp Lo Stop: When Pp value ≤ Pp Lo Stop value, the pump drive will stop running.
•UV Hi Stop: When AU value of UV1 is ≥ UV Hi Stop value the system will shut down.
•UV Hi Alarm: When AU value of UV1 is ≥ UV Hi Alarm value the pump drive will alarm but continue running.
•Perm Hi Alarm:When permeate scale reading is ≥ Perm Hi Alarm value the system will alarm but continue running.
•Perm Hi Stop: When permeate scale reading is ≥ Perm Hi Stop value the system will shut down.
•Feed Hi Alarm: When feed scale reading is ≥ Feed Hi Alarm value the system will alarm but continue running.
•Feed Hi Stop: When feed scale reading is ≥ Feed Hi Stop value the system will shut down.
•Feed Lo Alarm:When feed scale reading is ≤ Feed Lo Alarm value the system will alarm but continue running.
•Feed Lo Stop: When feed scale reading is ≤ Feed Lo Stop value the system will shut down.
•Silent Alarm:Set to “On” to silence alarms, set to “Off” to play alarm sound.
VPT Device
(FlowVPX)
Feed Reservoir
Extra Feed Reservoir
Constant
Feed Scale
KR2i Pump
Diafiltration Pump
(Auxiliary Pump 2)
Permeate
Scale
Permeate Reservoir
Module
Diafiltration Buffer
Reservoir

20
DOC0304
eRev. [Major Version]1.01.0 8/9/2023
User Guide
KrosFlo® KR2i Real-Time Process Management (RPM™) System
1. To enable an alarm, highlight the alarm value and click Enter.
2. Use the directional keys to set the desired alarm value, then click Enter to save the setting.
3. To disable an alarm, change the value to 0 and TFF system will read the alarm as “OFF”.
7.10 Tubing Calibration
Note: Condition tubing by running at ½ of maximum flow rate of tubing being calibrated for at least 10-15 minutes before conducting
tubing calibration. Tubing calibration cannot be done when pump is in RPM mode.
1. Select tubing size in Calibration menu.
2. Press CAL, the system set calibration volume will appear.
3. Press START / STOP—the pump will use its stored memory to dispense the specified calibration sample quantity. The pump
will stop automatically.
4. Weigh/measure the sample.
5. Use directional keys to correct the volume on the flashing display.
6. Press the Enter key to save the calibration setting. Once calibrated, a lower case "c" appears next to the tubing (e.g. 73c).
7. To confirm tubing calibration, press CHECK to dispense calibration volume using calibrated tubing and weigh dispensed
volume.
Note:If the adjusted calibration is high, "Err" will appear in the display. If this occurs, press the CAL control and repeat the calibration
procedure. The microprocessor will retain one special calibration value per tubing size, even when power is turned off. The next
calibration will replace the existing value.