REPLIGEN Spectrum KR2i User manual

Spectrum® KR2i/ KMPiTFF Systems
USER GUIDE AND OPERATING INSTRUCTIONS
Spectrum® KrosFlo® Research 2iTFF System
Spectrum® KrosFlo® MiniKros Pilot i TFF System

The information contained in this document is subject to change without notice.
Repligen Corporation makes no warranty of any kind with regard to this material, including, but not
limited to, the implied warranties of merchantability and fitness for a particular purpose.
Repligen Corporation shall not be liable for errors contained herein or for incidental or
consequential damages in connection with the furnishing, performance, or use of this material.
No part of this document may be photocopied, reproduced, or translated to another language
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For further information, please contact Repligen Corporation at www.repligen.com.
© 2018 Repligen Corporation. All rights reserved.
The trademarks mentioned herein are the property of Repligen Corporation and/or its affiliate(s) or
their respective owners.
Repligen’s TFF System (KR2i/ KMPi) meets strict quality control standards and is warranted against
defects in material and workmanship for a period of two (2) years from date of shipment.
The information contained herein is believed to be accurate and is offered in good faith for the
convenience of the user. PRODUCTS ARE FURNISHED UPON THE CONDITION THAT THE USER
ASSUMES ALL RISKS AND LIABILITIES AND THAT NEITHER THE SELLER NOR MANUFACTURER SHALL
BE LIABLE FOR ANY LOSS OR DAMAGE, DIRECT OR CONSEQUENTIAL, ARISING FROM THE USE OF
THESE PRODUCTS.
Spectrum®, KrosFlo®, MicroKros®, MidiKros® and MiniKros® are registered trademarks of Repligen
Corporation. Microsoft®, Windows® and Excel® are registered trademarks of Microsoft Corporation.
C-Flex® and Pharmapure® are registered trademarks of Saint-Gobain. MasterFlex® is a registered
trademark of Cole-Parmer. Trademarks bearing the ® symbol in this publication are registered in the
U.S. and in other countries.
This document copyright © 2018 Repligen Corporation. All rights reserved. Reproduction prohibited
except by permission of the copyright owner.
Visit http://spectrumlabs.com/filtration/ProcessSystems.html for more information.
Customer Support
[e-mail]
310-885-4600
Technical Support
[e-mail]
Spectrum A Repligen Brand
18617 S Broadwick St.
Rancho Dominguez, CA 90220
www.spectrumlabs.com

Table of Contents
Introduction .........................................................................................................................6
Safety Precautions................................................................................................................7
Explanation of Symbols: .....................................................................................................................7
WARNING: Product Use Limitation ....................................................................................................8
Specifications .......................................................................................................................8
KR2i Specifications..............................................................................................................................8
KMPi Specifications ............................................................................................................................9
KONDUiT Specifications....................................................................................................................10
System Configuration and Major Components..................................................................... 12
4.1 Pump Drive, Pump Head, & Integrated Pressure Monitor ......................................................12
4.2 Mounting Hardware.................................................................................................................12
4.3 Octopus Cables ........................................................................................................................12
System Parts List.................................................................................................................12
Materials of Construction ...................................................................................................13
Setup and Operation ..........................................................................................................13
7.1 Basic Setup...............................................................................................................................13
7.2 Manual Mode Setup ................................................................................................................15
7.3 C. Mode Setup..........................................................................................................................16
7.4 C/D and C/D/C Mode Setup.....................................................................................................16
7.5 C/D/D/C Mode Setup...............................................................................................................17
7.6 CFC Mode Setup.......................................................................................................................18
7.7 CF/D/C Mode Setup .................................................................................................................19
7.8 Ctrl Mode .................................................................................................................................20
7.9 System Setup Using KF Comm Pump Control Window ...........................................................20
7.9.1 KF Comm User Interface ...............................................................................................20
7.9.2 Entering System Settings ..............................................................................................23
7.10 Using Pump Interface...............................................................................................................30
7.10.1 Keypad...........................................................................................................................30
7.10.2 Main Screen ..................................................................................................................31
7.10.3 Remote Control Of Auxiliary Pumps and Valves...........................................................32
7.10.4 System Settings.............................................................................................................35
7.10.5 Hardware Setup ............................................................................................................35
7.11 Pressure Calibrate....................................................................................................................37
7.12 Alarms and Stops .....................................................................................................................37
7.13 Tubing Calibration....................................................................................................................38
System Functionality ..........................................................................................................39
8.1 Pump Controls .........................................................................................................................39
8.1.1 Overview .......................................................................................................................39

8.2 Detailed Pump Mode Operation..............................................................................................40
8.2.1 Concentration Mode.....................................................................................................40
8.2.2 Concentration/Diafiltration Mode................................................................................41
8.2.3 Concentration 1/Diafiltration/Concentration 2 Mode .................................................43
8.2.4 Concentration 1/Diafiltration 1/Diafiltration 2/Concentration 2 Mode.......................44
8.2.5 Constant Feed Concentration Mode.............................................................................45
8.2.6 Constant Feed/Diafiltration/Concentration Mode .......................................................46
8.2.7 Ctrl Mode ......................................................................................................................47
8.3 Running the TFF System...........................................................................................................48
8.4 Pump Mode Status Timeout Display........................................................................................48
Pump Head Setup and Operation ........................................................................................49
9.1 Introduction .............................................................................................................................49
9.2 Specifications ...........................................................................................................................49
9.3 KR2i Pump Head Operation .....................................................................................................52
9.4 KMPi Pump Head Operation....................................................................................................55
Auxiliary Component Setup and Operation.......................................................................... 58
10.1 Auxiliary Scales.........................................................................................................................58
10.1.1 Installation ....................................................................................................................58
10.2 Auxiliary Pumps........................................................................................................................59
10.2.1 Installation ....................................................................................................................59
10.2.2 Auxiliary Pump Settings ................................................................................................61
10.3 Automatic Backpressure Valve ................................................................................................61
10.3.1 Installation ....................................................................................................................61
10.3.2 Operation ......................................................................................................................62
10.3.3 Automatic Backpressure Valve Settings........................................................................62
10.4 KONDUiT ..................................................................................................................................62
10.4.1 System Configuration and Major Components.............................................................62
10.4.2 Installation ....................................................................................................................63
10.4.3 Software/Firmware Operation......................................................................................65
10.4.4 KONDUiT Software/Firmware Configuration................................................................65
10.4.5 Software Operation.......................................................................................................65
10.4.6 Firmware Operation......................................................................................................66
10.4.7 Maintenance .................................................................................................................68
KF Comm Software .............................................................................................................68
11.1 Introduction .............................................................................................................................68
11.2 Installing and Updating TFF System Software and Firmware with KF Comm Software ..........69
11.3 USB Connection .......................................................................................................................70
11.4 Opening and Configuring the KF Comm Workbook Template ................................................71
11.5 Data Collection Tools ...............................................................................................................72

11.6 Worksheets Overview..............................................................................................................73
Basic Concepts of Tangential Flow Filtration........................................................................ 74
12.1 Introduction .............................................................................................................................74
12.2 Concentration ..........................................................................................................................74
12.3 Diafiltration..............................................................................................................................75
Troubleshooting .................................................................................................................75
Replacement and Auxiliary Parts.........................................................................................76
Index..................................................................................................................................77

6400-12355-000 Rev. 03
KR2i / KMPi TFF Systems
Introduction
Introduction
The KR2i[KrosFlo® Research 2 integrated] and KMPi[KrosFlo® MiniKros® Pilot integrated] Tangential
Flow Filtration (TFF) Systems are ideal automated pump systems for processes ranging from 1ml to
10L and 0.5L to 500L respectively. The systems consist of a digital peristaltic pump, man/machine
interface with graphical LCD display, Pump Head, digital readouts of pressure values with automated
shut-off controls, module stand, and real-time data collection software KF Comm. The KR2ican
mount up to two 2.3 LPM KR2iEasy-Load Pump Heads or two 2.9 LPM KR2iHigh-Performance Pump
Heads. The KMPican mount up to two 13 LPM KMPiEasy-Load Pump Heads or one 17 LPM KMPi
High-Performance Pump Head.
When equipped with optional auxiliary components, the TFF system's automated functions are
extended further. Through the combination of specific set-points measured through the TFF
system's auxiliary pumps, scales, KONDUiT and backpressure valves, the TFF system is able to carry
out complicated TFF processes with improved accuracy and efficiency.
The peristaltic pump handles abrasive slurries, corrosive fluids and highly viscous fluids with minimal
wear. The pump also allows only the tubing and other tube material to come in contact with the
fluid, has no valves to clog, and has smooth and easy-to-clean inner surfaces. Its performance has
proven that the bidirectional and self-priming TFF system has low shear, is capable of running dry
and pumping fluids with high quantities of entrained air, has high volumetric efficiency, and a motor
speed repeatability of 0.1 percent to maximize productivity.
The KR2iis the ideal system for small volume, R&D scale microfiltration and ultrafiltration due to its
compact footprint and versatility. The KMPihas been designed to be used with Spectrum’s
ProConnex® Flowpaths, which are sterile, fully-assembled and disposable process flowpaths for TFF.
The KMPiand Pro-Connex sets are ideal for upstream TFF microfiltration and downstream TFF
ultrafiltration. The disposable or dedicated flowpath—including the filter, pressure transducers,
tubing and fittings—eliminates the possibility of cross contamination. TangenX flat-sheet TFF
cassettes can also be used on both the KR2iand KMPi.
The hollow fiber (HF) membrane modules provide excellent performance for sample concentration,
fractionation and washing while avoiding membrane fouling and maximizing product recovery.
TangenX cassettes are ideal for applications where the inner screen increases turbulence and gives
high permeate flux for viscous proteins, oligosaccharides, and viruses for example. The disposability
of both the cassettes and HF filters eliminates not only the potential for cross-contamination and
costs associated with cleaning and rinsing, but also the difficulties associated with validating
reusable membranes. Used in conjunction with HF membrane modules, or TangenX cassettes, the
TFF systems offer the following advantages:
•Faster processing times
•Superior filtration dynamics
•Filter disposability
•Lower costs

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KR2i / KMPi TFF Systems
Safety Precautions
•Direct and easy scale-up for production volumes
Safety Precautions
DANGER: High voltages exist and are accessible. Use extreme caution when servicing
internal components.
Remove power from the pump before any cleaning operation is started.
WARNING Remove power from the pump before attempting any maintenance.
WARNINGS: Tubing breakage may result in fluid being sprayed from pump. Use
appropriate measures to protect operator and equipment.
Turn drive off before removing or installing tubes. Fingers or loose clothing
could get caught in drive mechanism.
CAUTIONS: Power must be turned off before connecting the external remote control
cable to prevent damage to the drive.
Do not contaminate the lubricant in the container, on the shaft or on the
seal with foreign material. Failure to observe this precaution may result in
damage to the seal and premature failure of the seal.
No foreign matter should be allowed under the gasket on the back of the
front plate or under the heads of the screws. Failure to observe this
precaution may result in leakage during washdown of the drive.
CAUTION: To avoid electrical shock, the power cord protective grounding conductor
must be connected to ground. Not for operation in wet locations as
defined by EN61010-1.
CAUTION: Keep fingers away from rotor while pump is in operation. Stop pump
before loading or unloading tubing.
Explanation of Symbols:
CAUTION: Risk of Danger. Consult Operating Instructions for nature of hazard and
corrective actions.
CAUTION: Risk of crushing. Keep fingers away from rotor while pump is in operation.
Stop pump before loading or unloading tubing.
CAUTION: Hot surface. Do not touch.
CAUTION: Risk of electric shock. Consult Operating Instructions for nature of hazard
and corrective actions.

8400-12355-000 Rev. 03
KR2i / KMPi TFF Systems
Specifications
WARNING: Product Use Limitation
Risk of Danger. Consult Operating Instructions for nature of hazard and
corrective actions.
This product is not designed for, nor intended for use in patient connected
applications; including, but not limited to, medical and dental use, and
accordingly has not been submitted for FDA approval.
This product is not designed for, nor intended for use in hazardous duty
areas as defined by ATEX or the NEC (National Electrical Code); including,
but not limited to use with flammable liquids. Consult the factory for
products suitable for these types of applications.
ENVIRONMENTAL Waste electrical products should not be disposed of with household
PROTECTION: waste. Please recycle where facilities exist. Check with your Local
Authority or retailer for recycling advice.
Specifications
KR2iSpecifications
Output
Speed: 0.1 to 600 rpm
Torque output, Maximum: 180 oz-in (13 kg•cm)
540 oz-in Starting
Speed regulation: Line ±0.1% F.S.
Load ±0.1% F.S.
Drift ±0.1% F.S.
Display: 128 x 64 LCD w/ LED Backlight
Pressure Sensor Limits: -9.99 to 75 psi
Input
Supply voltage limits: 90 to 260 Vrms @ 50/60 Hz
(Universal Input)
Current, Maximum: 2.2A @ 115 Vrms, or 1.1A @ 230 Vrms
Construction
Dimensions (L × W × H): 10.5 in × 8 in × 8 in
(267 × 203 × 203 mm)
Weight: 13 lb (5.9 kg)
Enclosure Rating: IP33
Environment

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KR2i / KMPi TFF Systems
Specifications
Temperature, Operating: 0° to 40°C (32° to 104°F)
Temperature, Storage: –25° to 65°C (–13° to 149°F)
Humidity (non-condensing): 10% to 90%
Altitude: Less than 2000 m
Pollution Degree: Pollution Degree 2
Compliance:(For ETL Mark):
UL 61010-1, CAN/CSA C22.2 No. 61010-1
(For CE Mark):
EN61010-1 (EU Low Voltage Directive) and
EN61326 (EU EMC Directive)
RoHS
KMPiSpecifications
Output
Speed:
All models 0.1 to 650 rpm
Torque output, max.
All models 520 oz-in (37.4 kg • cm)
1560 oz-in Starting
Speed regulation:
All models Line ±0.1% F.S.
Load ±0.1% F.S.
Drift ±0.1% F.S.
Display:
All models 128 x 64 LCD w/ LED Backlight
Remote outputs:
All models Voltage speed output
(0-10V DC @ 1 kΩ min)
All models Current speed output
(0-10 mA @ 0-600Ω)
All models Tach output
(100 to 6500 Hz, 50% duty cycle, 10 Hz/rpm)
All models Motor running output
(N.O. & N.C. contact closure, 1A @ 28V DC)
Input
Supply voltage limits:
All models 90 to 260 Vrms @ 50/60 Hz
(Universal Input)
Current, max.:
All models 4.5A @ 115 Vrms, or 2.6A @ 230 Vrms
Remote Inputs:
All models START/STOP, CW/CCW, PRIME
(Contact closure)
All models Voltage input (0-10V DC @ 10 kΩ),
±50V common mode range

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KR2i / KMPi TFF Systems
Specifications
All models Current input
(0-20 mA or 4-20 mA @ 250 Ω),
±50V common mode range
Construction
Dimensions (L × W × H): 17.5 in × 11 in × 13 in
(445 × 280 × 330 mm)
Weight: 40 lb (18 kg)
Enclosure Rating:
All models NEMA 4X
Environment
Temperature, Operating:
All models 0° to 40°C (32° to 104°F)
Temperature, Storage:
All models -25° to 65°C (-13° to 149°F)
Humidity (non-condensing):
All models 10% to 100%
Altitude:
All models Less than 2000 m
Noise Level: <70dBa @ 1 meter
Pollution Degree:
All models Pollution Degree 3
(Indoor use — Sheltered locations)
Chemical Resistance:
Models with stainless steel Exposed material is 316 stainless steel enclosure and hard coat
anodized aluminum
Models with powder coated Exposed material is polyester coated
steel enclosure steel and hard coat anodized aluminum
Compliance: Conforms to ANSI/UL Std 61010-1
Certified to CAN/CSA Std C22.2 No. 61010-1
This product has been tested to the
requirements of CAN/CSA-C22.2 No. 61010-1
second edition, including Amendment 1, or
a later version of the same standard
incorporating the same level of testing requirements.
(For CE Mark):
EN61010-1: (EU Low Voltage Directive) and
EN61326: (EU EMC Directive)
KONDUiT Specifications
Dimensions (L x W x H): 7 ¾” x 4 ¾” x 4 ½”
Weight: 1.6 kg
Power Supply Range:
a. Input Power: 100-240 VAC

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KR2i / KMPi TFF Systems
Specifications
b. Input Frequency: 47-63 Hz
c. Input Current: ~0.4A
KONDUiT Power Requirements
a. Voltage: 24 VDC
b. Current: 0.625 A
Environment
Temperature, Operating: 2°C to 50°C
Temperature, Storage: -25°C to 65°C
Chemical Resistance: Powder-coated aluminum, urethane
Pressure Range: Rated for pressure up to 75 psi (5 bar)
Readability
Conductivity
Range: 0.1 to 100 mS/cm
Accuracy: From 0.1 to 2 mS/cm ± 0.1 mS/cm; 2 to 50 mS/cm ±5% of reading; 50 to 100 mS/cm
typically ±5% of reading
Temperature
Range: 0-70°C
Accuracy: Better than ±0.2°C (typically better than 0.1°C)
Optional: UV
Output signal: 4-20 mA sourcing with 400 ohm maximum at 24 VDC; scaled to 0-2 AU with
repeatability of 1% of full scale (0.02 AU)
Typical Response Time: 1 second
Maximum Zero Shift: <2% of full scale (<0.040 AU)
Long Term Output Drift: <5% per month of full scale (<0.100 AU)
Compliance: Conforms to ANSI/UL Std 61010-1
Certified to CAN/CSA Std C22.2 No. 61010-1
This product has been tested to the
requirements of CAN/CSA-C22.2 No. 61010-1
second edition, including Amendment 1, or

12 400-12355-000 Rev. 03
KR2i / KMPi TFF Systems
System Configuration and Major Components
a later version of the same standard
incorporating the same level of testing requirements.
(For CE Mark):
EN61010-1: (EU Low Voltage Directive) and
EN61326: (EU EMC Directive)
System Configuration and Major Components
4.1 Pump Drive, Pump Head, & Integrated Pressure Monitor
The TFF system comes with the Digital Pressure Monitor integrated into the Pump Drive as one unit.
The internal microprocessor runs both the Pump and Pressure monitor functions.
4.2 Mounting Hardware
The KMPiTFF system comes with the Easy-Load Pump Head that requires no extra hardware to
mount. The KR2iTFF system comes with an attached mounting plate for the KMPiEasy-Load Pump
Head.
4.3 Octopus Cables
The TFF system comes with two Octopus Cables—one with more pins for the Auxiliary Components,
and one with less pins for the Pressure Transducers. On the back of the TFF system are two Interface
ports for the Octopus Cables.
System Parts List
KR2iTFF System
Part Number Pump Description
SYR2-U20 Complete KR2i System
SYR2-U10 KR2iPump Drive, Head, and Stand Only
System Parts List:
Part Description Quantity
KrosFlo® Research 2iPump Drive w/ Integrated Pressure Monitor 1
KrosFlo KF Comm Software Suite (on USB) 1
Auxiliary Component Octopus Cable 1
Pressure Transducer Cable 1
Power Supply Cable 1
KrosFlo® Research II Easy-Load Pump Head 1
KMPiTFF System
Part Number Pump Description
SYR2-U20 Complete KMPiSystem
SYR2-U10 KMPiPump Drive and Head Only

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KR2i / KMPi TFF Systems
Materials of Construction
System Parts List:
Part Description Quantity
KMPiPump Drive w/ Integrated Pressure Monitor 1
Auxiliary Component Octopus Cable 1
Pressure Transducer Octopus Cable 1
Power Supply Cable 1
KMPi13LPM Easy-Load Pump Head 1
KMPiFittings Kit 1
KrosFlo Module Accessory Kit 1
SAW Scale 12" x 12" 60kg x 1g 1
10L Process Reservoir 1
PharmaPure #26 Tubing Pack 1
PharmaPure #73 Tubing Pack 1
Automatic Backpressure Valve 1
Process System Computer 1
KrosFlo KF Comm Software Suite (on USB) 1
Materials of Construction
Part Number Material
TFF System (Product Contact Surfaces)
Tubing / Reservoir Closures C-Flex® / Pharmapure®
Reservoirs Polypropylene
Disposable Pressure Transducers Polysulfone
Plastic Fittings Polypropylene / Polysulfone
Hollow Fiber Module (Module Sold Separately)
Membrane ME, mPES, PES or PS
Housing Pigmented and Non-pigmented Polysulfone
Potting Polyurethane / Epoxy
End-caps Pigmented Polysulfone
Setup and Operation
7.1 Basic Setup
NOTE: See Section 8.2 for Sample Applications.
1. Mount the TFF system on a flat, horizontal surface with no more than two Pump Heads
attached (see Section 9 for Pump Head Setup details)
2. Connect both Octopus Cables to the back of the TFF system
3. Connect up to three pressure transducers to the Pressure Transducer Octopus Cable ports as
suitable for application

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KR2i / KMPi TFF Systems
Setup and Operation
4. Connect one (if using KR2i) or up to two (if using KMPi) Automatic Backpressure Valves
(ABV's) to the Auxiliary Component Octopus Cable's "Valve 1" ("Valve" for KR2i) and/or
"Valve 2" (see Section 10.3 for ABV Setup details)
5. Connect power cable to the TFF system
6. Follow guidelines and diagrams below to determine which Auxiliary Components are
required to operate specific Pump Modes for manual, semi-automated, and automated
processes (see Section 8 for Pump Mode Setup details)
i. If using Auxiliary Pump, configure Auxiliary Pump before starting application (see
Section 10.2 for Auxiliary Pump Setup details)
ii. After connecting Auxiliary Components, power on the TFF system first before
powering on Auxiliary Components
7. Connect TFF flowpath to TFF system
8. Set low and high pressure alarms and interlocks as required by the process conditions
9. Input Concentration Factor/Diafiltration Volume (CF/DV) set-points into the TFF system’s
Pump Mode settings to start application
Auxiliary Components Required for Pump Modes
Pump Mode Feed Scale Permeate Scale Auxiliary Pump 1 Auxiliary Pump 2
M Mode
C Mode X* XX**
C/D Mode X X XX**
C/D/C Mode XXX X
C/D/D/C Mode XXXX
CFC Mode XXXX**
CF/D/C Mode XXXX
Ctrl Mode XXX**
* The Feed Scale is only required in applications using C Mode if user does not want to input
starting Feed Scale values
** Auxiliary Pump 2 is optional for applications that utilize it as a Permate Pump, such as MF
applications
NOTE: All diagrams in Section 7 are shown with the process-scaled KMPi, however the same set-ups
may be used with the KR2i.

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KR2i / KMPi TFF Systems
Setup and Operation
7.2 Manual Mode Setup
NOTE: Valves are intentionally left out of the diagrams below for visual clarity.
1. Any combination of Auxiliary Components is possible in Manual Mode—the only required
component is the TFF system pump itself. Diagram above is a full setup with all Auxiliary
Components
2. Auxiliary Pumps
a. Connect up to two auxiliary pumps to the Auxiliary Component Cable’s “Auxiliary
Pump 1” and/or “Auxiliary Pump 2” ports
b. Connect auxiliary pump power cable(s)
3. Auxiliary Scales
a. Connect up to two scales to the Auxiliary Component Cable’s “Feed Scale” and/or
“Permeate Scale” ports
Feed Scale
Permeate Pump
(Auxiliary Pump 2)
Permeate Scale
Diafiltration Buffer Reservoir
Module
Permeate Reservoir
Feed Reservoir
Diafiltration Pump
(Auxiliary Pump 1)
KMPiPump

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Setup and Operation
7.3 C. Mode Setup
1. Auxiliary Pumps
a. (Optional) To control permeate rate, connect auxiliary pump to the Auxiliary
Component Cable’s “Auxiliary Pump 2” port
b. (Optional) Connect Auxiliary Pump 2's power cable
2. Auxiliary Scales
a. Connect scale to the Auxiliary Component Cable’s “Permeate Scale”
7.4 C/D and C/D/C Mode Setup
NOTE: Permeate Pump is optional and shown in the diagram as an example for applications that
require permeate control.
1. Auxiliary Pumps
Feed Reservoir
KMPiPump
Permeate Reservoir
Diafiltration Buffer Reservoir
Module
Diafiltration Pump
(Auxiliary Pump 1)
Feed Scale
Permeate Reservoir
Permeate Pump
(Auxiliary Pump 2)
Permeate Scale
Module
KMPiPump
Feed Reservoir
Permeate Scale

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Setup and Operation
a. Connect auxiliary pump to the Auxiliary Component Cable’s “Auxiliary Pump 1” port
and second auxiliary pump to the Auxiliary Component Cable's "Auxiliary Pump 2"
port
b. Connect Auxiliary pump power cables(s)
2. Auxiliary Scales
a. Connect first scale to the Auxiliary Component Cable’s “Feed Scale” port and second
scale to the Auxiliary Component Cable’s “Permeate Scale” port
7.5 C/D/D/C Mode Setup
1. Auxiliary Pumps
a. Connect first auxiliary pump to the Auxiliary Component Cable’s “Auxiliary Pump 1”
port and second auxiliary pump to the Auxiliary Component Cable’s “Auxiliary Pump
2” port
b. Connect auxiliary power cables
c. Auxiliary Pump 1 will function as Diafiltration Pump 1, and the Auxiliary Pump 2
will function as Diafiltration Pump 2
2. Auxiliary Scales
a. Connect first scale to the Auxiliary Component Cable’s “Feed Scale” port and second
scale to the Auxiliary Component Cable’s “Permeate Scale” port
Diafiltration Buffer Reservoir 1
Module
Permeate Reservoir
Diafiltration Buffer
Reservoir 2
Feed Reservoir
KMPiPump
Feed Scale
Permeate Scale
Diafiltration Pump
(Auxiliary Pump 1)
Diafiltration Pump
(Auxiliary Pump 2)

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Setup and Operation
7.6 CFC Mode Setup
NOTE: Permeate Pump is optional and shown in the diagram as an example for applications that
require permeate control.
1. Auxiliary Pumps
a. Connect auxiliary pump to the Auxiliary Component Cable's "Auxiliary Pump 1" port
and second auxiliary pump to the Auxiliary Component Cable's "Auxiliary Pump 2"
port
b. Connect auxiliary pump power cables
c. The Auxiliary Pump 1 will function as Constant Feed Pump
2. Auxiliary Scales
a. Connect first scale to the Auxiliary Component Cable’s “Feed Scale” port and second
scale to the Auxiliary Component Cable’s “Permeate Scale” port
Extra Feed Reservoir
Module
Feed Reservoir
Permeate Reservoir
Diafiltration Pump
(Auxiliary Pump 1)
KMPiPump
Permeate Pump
(Auxiliary Pump 2)
Permeate Scale
Feed Scale

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Setup and Operation
7.7 CF/D/C Mode Setup
1. Auxiliary Pumps
a. Connect auxiliary pump to the Auxiliary Component Cable's "Auxiliary Pump 1" port
and second auxiliary pump to the Auxiliary Component Cable's "Auxiliary Pump 2"
port
b. Connect auxiliary pump power cables
c. Auxiliary Pump 1 will function as Constant Feed Pump, and Auxiliary Pump 2 will
function as Diafiltration Pump
2. Auxiliary Scales
a. Connect first scale to the Auxiliary Component Cable’s “Feed Scale” port and second
scale to the Auxiliary Component Cable’s “Permeate Scale” port
KMPiPump
Diafiltration Pump
(Auxiliary Pump 2)
Feed Scale
Diafiltration Buffer Reservoir
Permeate Scale
Constant Feed Pump
(Auxiliary Pump 1)
Extra Feed Reservoir
Permeate Reservoir
Feed Reservoir
Module

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KR2i / KMPi TFF Systems
Setup and Operation
7.8 Ctrl Mode
NOTE: Refer to section 8.2.1.7 and 8.3 for further information about Ctrl Mode.
1. Auxiliary Pumps
a. Connect auxiliary pump (optional permeate pump) to the Auxiliary Component
Cable’s “Auxiliary Pump 2” port
d. Connect auxiliary pump power cables
2. Auxiliary Scales
a. Connect first scale to the Auxiliary Component Cable’s “Feed Scale” port and second
scale to the Auxiliary Component Cable’s “Permeate Scale” port
7.9 System Setup Using KF Comm Pump Control Window
7.9.1 KF Comm User Interface
The Pump Control Interface can be used to interact with all the features of the system. This screen
will appear when the KF Comm software is opened in Excel. When needed, the functions and
setpoints are accessible on the main pump display. For full explanation of Pump Control window
please refer to section 8.1 KF COMM Pump Control User Interface.
Below is the basic layout of the Pump Control window:
Feed Reservoir
KMPiPump
Permeate Reservoir
Diafiltration Buffer Reservoir
Module
Feed Scale
Permeate Pump
(Auxiliary Pump)
Permeate Scale