Knauer Azura Assistant ASM 2.1L User manual

Assistant ASM 2.1L
User Manual
HPLC

2
Note: Only use the device for applications that fall within the range of the
intended use. Otherwise, the protective and safety equipment of the
device could fail.
Manuel en français: Si jamais vous préfériez un manuel en français pour ce produit, veuillez
vous contacter le support technique (Technical Support) par email ou par
fax avec le no. de série. Merci beaucoup.
Technical Support: Phone: [phone] (9-17h, Central European Time)
Fax: [phone]
E-Mail: [email protected]
Languages: German, English
Publisher: KNAUER Wissenschaftliche Geräte GmbH
Hegauer Weg 38
D-14163 Berlin
Phone: [phone]
Fax: [phone]
Internet: www.knauer.net
E-Mail: [email protected]et
Version information: Article number: V6815
Version number: 3.0
Last update: 2017/04/13
Translation of the original edition
The information in this document is subject
to change without prior notice.
For latest version of the manual, check our website:
http://www.knauer.net/knowledge.html
Copyright: This document contains confidential information and may not be repro-
duced without written content of KNAUER Wissenschaftliche Geräte
GmbH.
© KNAUER Wissenschaftliche Geräte GmbH 2017
All rights reserved.
AZURA® is a registered trademark of
KNAUER Wissenschaftliche Geräte GmbH.

ASM 2.1L user manual V6815
3TableofContents
Table of contents
Product information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Device overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Detector and flow cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Pump and pump heads . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Valve drives and valve heads . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Degasser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Configuration of modules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Maximum number of same modules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Limitation of combination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Position of modules and IP port connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
One module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Two modules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Three modules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Software functions of different configurations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
ASM 2.1L configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Single device configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Scope of delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Safety for users. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Signal words . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Symbols and signs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Unpacking and setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Contacting the Technical Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Location requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Connectors on the rear side . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Controlling with the pin header . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Integrator output . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Initial startup. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
PEEK fittings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
ASM 2.1L as Compact HPLC isocratic . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Leak management . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Connecting a device to a computer to a Local Area Network (LAN) . . . . . . . . . . . . . . . . . . 18
Configuring the LAN Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Connecting the Cables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Configuring the Router . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Integrating the LAN into a Company Network . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Controlling Several Systems Separately in a LAN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Meaning of the LEDs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Meaning of the degasser LEDs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Startup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Functionality tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

ASM 2.1L user manual V6815
Table of Contents 4
Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
LAN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
System messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Maintenance and care . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Maintenance contract . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Required system information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Cleaning and caring for the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Maintenance tasks for users . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Communication . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
General . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Repeat orders . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Legal information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Transport damage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Warranty conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Declaration of conformity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Abbreviations and terminology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

ASM 2.1L user manual V6815
5 Product information
Product information
Note: Only use the device for applications that fall within the range of the
intended use. Otherwise, the protective and safety equipment of the
device could fail.
Assistant ASM 2.1L The Assistant ASM 2.1L is a compact combination module which is used in
analytical, preparative and continuous liquid chromatography. The con-
cept of flexible combination of device modules combines highest func-
tionality with minimal space requirements.
Equipped with up to three modules the assistant fulfills different custom-
ized or preset tasks like sample and buffer selection, sample injection, col-
umn switching, fraction collection. A selection of four different device
types is available: valve, pump, UV detector, degasser. Depending on the
selected modules the assistant is either a stand-alone compact system or
a part of an advanced system.
Compact HPLC With a suitable selection of micro devices the ASM 2.1L can be used as a
supplementary module for the HPLC or it can be combined to a compact
HPLC system for chromatographic analyses in the high pressure range.
Operating range The device can be used in the following areas:
Biochemistry analyses
FPLC/Biopurification
Chemical analyses
Food analyses
Pharmaceutical analyses
Environmental analyses
Eluents Small quantities of substances such as additives, modifiers, or salts can
influence the durability of the materials. For further reference on chemical
compatibility, please consult the user manuals of the integrated devices
(see “Device overview” on page 5).
Device overview
As an additional module for the HPLC system, the ASM 2.1L can be
equipped with different devices on request. If the ASM 2.1L is delivered
with 2 micro devices and if the left position from front is left empty, the
micro device located in the central position is represented as left device in
the software packages.
Following devices can be integrated:
Detector UVD 2.1S Small variable single wavelength UV detector
A wide range of flow cells for analytical or preparative LC applications
with flow rates from 10 μl/min to 10 l/min.
Automatic recognition and storing of device-specific information,
which are important for Good Laboratory Practice, operation qualifica-
tions, or repairing the device.
Pump P 2.1S/P 4.1S Dual-piston technology
Liquid transport with stable flow rate and high flow accuracy
Long service life
10 ml and 50 ml pump head selectable
With or without pressure sensor
As a material for the pump head interior titanium or stainless steel can
be chosen. For the 10 ml pump head ceramic can be chosen.
Valves The valves fall into different categories:
2-position valves with 6 ports and 8 ports
2-postion valves are used to inject the sample from the sample loop in

ASM 2.1L user manual V6815
Device overview 6
the pumps high pressure flow, so that the sample is transported to the
column.
Multiposition valves with 6 ports and 8 ports
Multiposition valves are used to switch fluid streams, e.g. the selection
of eluents, columns or for fractionating of eluate. Bioninert valve heads
are available.
Valve heads available for different applications in UHPLC, analytical
HPLC and preparative HPLC.
Different valves of the company VICI Valco Instruments Co.Inc. can be
installed.
Valve drives 6V, 12V, 16V availabe.
Degasser The usage of a degasser in front of the pump leads to a stable baseline
of the chromatogram.
Eluents must not be degassed before they be used in the HPLC.
Analytical flow rate up to 10 ml/min
Detector and flow cells
The ASM 2.1L can be equipped with a detector on the left side. The
UVD 2.1S will be delivered with a test cell. For the use of the detector, a
flow cell has to be installed. For further information, take a look into the
UVD 2.1S user manual V6820: http://www.knauer.net/fileadmin/user_u-
pload/produkte/files/Dokumente/detectors/azura/manuals/V6820_A-
ZURA_UVD_2.1S_User-Manual_EN.pdf
Flow cells Flow cells are available with different path lengths, connections and also
for different maximum flow rates. The flow cells are made of stainless steel
or PEEK.
Pump and pump heads
The ASM 2.1L can be equipped with a pump P 2.1S/P 4.1S. The pump
P 4.1S is equipped with an additional pressure sensor. For further infor-
mation, take a look into the P 2.1S/P 4.1S user manual V6870: http://
www.knauer.net/fileadmin/user_upload/produkte/files/Dokumente/
pumps/azura/manuals/V6870_P_4.1S-P_2.1S_User-Manual_EN.pdf
In case an ASM 2.1L with a SmartMix is used and you are looking for fur-
ther information, please consult the SmartMix user manual V5350A:
http://www.knauer.net/fileadmin/user_upload/produkte/files/Dokumente/
mixing_chambers/manuals/V5350A_SmartMix_Handbuch_EN.pdf
Path length Connectors Material Flow rate
Analytical flow cells 3–10 mm 1/16“ Stainless steel,
PEEK
20–50 ml/min
Preparative flow cells 0.5/1.25/2/
5 mm
1/4”, 1/8”, 1/16“ Stainless steel,
PEEK
250–10000 ml/
min

ASM 2.1L user manual V6815
7 Device overview
Pump heads The pump heads can be easily removed and replaced via four front-acces-
sible screws by the user.
Valve drives and valve heads
Prior to delivery the valve drive can be equipped with KNAUER multiposi-
tion valves and 2-position valves. Connections and channels of 2-position
valves are described by abbreviations (example 6P/3C=2-position valve
with 6 ports and 3 channels). In case of multiposition valves the central
port does not count. For further information, take a look into the V 2.1S
user manual V6860: http://www.knauer.net/fileadmin/user_upload/pro-
dukte/files/Dokumente/valves/azura/manuals/V6860_AZURA_V_2.1S_user-
manual_EN.pdf
The ASM 2.1L can be equipped with following valve heads.
Legend
1Pressure sensor
2Pump head
Fig. 1 Pump with pressure sensor in the ASM 2.1L
12
Pump head Maximum pump capacity
Stainless steel 10 ml
Titanium 10 ml
Stainless steel 50 ml
Titanium 50 ml
Ceramic 10 ml
Legend
1Valve head
Fig. 2 Valves in the ASM 2.1L
1

ASM 2.1L user manual V6815
Device overview 8
Degasser
The ASM 2.1L can be equipped with a degasser. By using a degasser up
to two eluents can be degassed simultaneously in a reliable and comfort-
able way. For further information, take a look into the DG 2.1S user man-
ual V6880: http://www.knauer.net/fileadmin/user_upload/produkte/files/
Dokumente/degassers/azura/manuals/V6880_AZURA_DG_2.1S_User-
Manual_EN.pdf
Degasser Gas Outlet If the ASM 2.1L is equipped with a degasser, the gas outlet (exhaust) will
be located on the front . Air and possibly solvent vapors will be pumped
out here. If necessary, it can be combined with a fume hood or similar
extraction device.
KNAUER Valves Ports Connection Material Pressure stability
2-position valve 6 1/16", 1/8" Stainless steel 300 bar
2-position valve 6 1/16" Stainless steel 400 bar
2-position valve 6, 8 1/16" Stainless steel 1000 bar
2-position valve 6 1/16", 1/8” PEEK 150 bar
Multiposition valve 6 1/16", 1/8" Stainless steel 300 bar
Multiposition valve 6 1/16" Stainless steel 400 bar
Multiposition valve 6,8 1/16" Stainless steel 1000 bar
Multiposition valve 6 1/16", 1/8" PEEK 150 bar
Multiposition valve 12 1/8" Stainless steel 25 bar
Multiposition valve 16 1/16" Stainless steel 50 bar
VICI Valves Ports Connection Material Pressure stability
2-position valve 6 1/16" Stainless steel 700 bar, 1000 bar
Multiposition valve 6 1/16" Stainless steel 700 bar, 1000 bar
Legend
1Degasser
Fig. 3 2-channel degasser in the ASM 2.1L
1

ASM 2.1L user manual V6815
9 Device overview
Configuration of modules
Maximum number of same modules
Three modules can be integrated in an assistant. The number of same
device types is limited:
1x Degasser
1x Detector UVD 2.1S
2x Pumps (only with ClarityChrom®or PurityChrom®), HPG is not sup-
ported
Limitation of combination
The pump P 4.1S cannot be placed to the right of detector UVD 2.1S,
because the fittings of the detector flow cell cannot be mounted.
An assistant with only degasser integrated is not allowed.
Position of modules and IP port connections
The positions of the modules are important to address them by IP ports,
which is required by the chromatography software PurityChrom® and by
single device configuration.
One module
Is one module in the assistant, it is always connected to the left (port
10002).
Two modules
For two modules in the assistant, port 10003 is not connected.
Fig. 4 ASM 2.1L gas outlet on the front
1

ASM 2.1L user manual V6815
Scope of delivery 10
Three modules
For three modules, the position is as follows: left module: port 10002,
middle module: port 10003, right module: port 10004.
Software functions of different configurations
ASM 2.1L configuration
Single device configuration
Scope of delivery
Note: Only use original parts and accessories made by KNAUER or a com-
pany authorized by KNAUER.
Devices ClarityChrom® OpenLab® Mobile Control
V4
Two pumps
(independent)
yes no no
Fractionvalve11no
Injection
module1
1. An injection module is a combination of one pump and one 6-port 2-position valve.
no no no
Devices ClarityChrom® OpenLab® Mobile Control
V4
Two pumps
(independent)
yes yes yes
Fraction valve 1 Cascading1
1. Multi valve fraction collector
1
Injection
module
no yes no
Delivery ASM 2.1L with devices
User manual ASM 2.1L plus user manuals of integrated micro
devices (German/English)
Power cable with kettle plug
AZURA® Accessories kit
Accessories kits of integrated micro devices

ASM 2.1L user manual V6815
11 Safety for users
Safety for users
Professional Group The user manual addresses persons who are qualified as chemical labora-
tory technicians or have completed comparable vocational training.
The following knowledge is required:
Fundamental knowledge of liquid chromatography
Knowledge regarding substances that are suitable only to a limited
extent for use in liquid chromatography
Knowledge regarding the health risks of chemicals
Participation during an installation of a device or a training by the com-
pany KNAUER or an authorized company.
If you do not belong to this or a comparable professional group, you may
not perform the work described in this user manual under any circum-
stances. In this case, please contact your superior.
Safety Equipment When working with the device, take measures according to lab regula-
tions and wear protective clothing:
Safety glasses with side protection
Protective gloves
Lab coat
What must be taken into
account?
All safety instructions in the user manual
The environmental, installation, and connection specifications in the
user manual
National and international regulations pertaining to laboratory work
Original spare parts, tools, and solvents made or recommended by
KNAUER
Good Laboratory Practice (GLP)
Accident prevention regulations published by the accident insurance
companies for laboratory work
Filtration of substances under analysis
Use of inline filters
Once they have been used, never re-use capillaries in other areas of
the HPLC system.
Only use a given PEEK fitting for one specific port and never re-use it
for other ports. Always install new PEEK fittings on each separate port.
Follow KNAUER or manufacturer’s instructions on caring for the
colums.
More safety-relevant information is listed below:
flammability: Organic solvents are highly flammable. Since capillaries
can detach from their screw fittings and allow solvent to escape, it is
prohibited to have any open flames near the analytical system.
solvent tray: Risk of electrical shock or short circuit if liquids get into the
device's interior. For this reason, place all bottles in a solvent tray.
solvent lines: Install capillaries and tubing in such a way that liquids
cannot get into the interior in case of a leak.
leaks: Regularly check if any system components are leaking.
power cable: Defective power cables are not to be used to connect the
device and the power supply system.
self-ignition point: Only use eluents that have a self-ignition point
higher than 150 °C under normal ambient conditions.
Installation Qualification Document (“IQ”, English)

ASM 2.1L user manual V6815
Symbols and signs 12
power strip: If several devices are connected to one power strip, always
consider the maximum power consumption of each device.
power supply: Only connect devices to voltage sources, whose voltage
equals the device's voltage.
toxicity: Organic eluents are toxic above a certain concentration.
Ensure that work areas are always well-ventilated! Wear protective
gloves and safety glasses when working on the device!
Where is use of the
device prohibited?
Never use the system in potentially explosive atmospheres without appro-
priate protective equipment. For further information, contact the Techni-
cal Support of KNAUER.
Secure decommissioning At any time, take the device completely out of operation by either switch-
ing off the power switch or by pulling the power plug.
Opening the Device The device may be opened by the KNAUER Technical Support or any
company authorized by KNAUER only.
Signal words
Possible dangers related to the device are divided into personal and
material damage in this user manual.
Lethal injuries will occur.
Serious or moderate injuries can occur.
Minor injuries can occur.
Device defects can occur.
Decontamination
Contamination of devices with toxic, infectious or radioactive substances
poses a hazard for all persons during operation, repair, sale, and disposal
of a device.
Life-threatening injuries
Health danger if getting in contact with toxic, infectious or radio-active
substances.
Before disposing of the device or sending it away for repair, you are
required to decontaminate the device in a technically correct manner.
All contaminated devices must be properly decontaminated by a special-
ist company or the operating company before they can be recommis-
sioned, repaired, sold, or disposed of. All materials or fluids used for
decontamination must be collected separately and disposed of properly.
Decontamination Report Devices without a completed Decontamination Report will not be
repaired. If you would like to return a device to KNAUER, make sure to
enclose a completed Decontamination Report with the device: http://
www.knauer.net/en/knowledge/downloads/service.html
Symbols and signs
The following table explains symbols and labels which are used on the
device, on the graphic user interface or in the user manual.
Symbol Meaning
Device fulfills the requirements of the
Conformité
Européenne
, which is confirmed by the Declaration of
Conformity.

ASM 2.1L user manual V6815
13 Unpacking and setup
Unpacking and setup
Contacting the Technical Support
You have various options to contact the technical support:
Phone [phone]
Fax [phone]
E-mail [email protected]
You can make your requests in English and German.
Location requirements
Only if the requirements for ambient conditions of the operating environ-
ment are met, can the intended use be ensured. Details on the operating
conditions can be found in the Technical Data section.
Device defect
The device overheats at exposure to sunlight and insufficient air circula-
tion. Device failures are very likely.
Set up the device in such a way that it is protected against exposure to
direct sunlight.
Keep at least 15 cm clear at the rear and 5–10 cm at each side for air cir-
culation.
Space Requirements At least 5 cm if another device is set up on one side
At least 10 cm if further devices are set up on both sides
At least 15 cm on the rear panel for the fan.
Leave the power plug on the rear of the device accessible to be able to
disconnect the device from the mains.
General requirements Position the device on a level and even surface.
Protect the device against direct exposure to sunlight.
Set up the device at a location not exposed to air drafts (A/C systems).
Do not set up the device in the vicinity of other machines that cause
floor vibrations.
Power supply The device is only suitable for alternating current. The supplied power
cable is to be used to connect the device to the mains supply.
Unpacking
Note: The leak sensor may malfunction if the device stands on a tilted sur-
face. Use a level to check that the device stands horizontally.
Store all packing materials. Included packing list should be kept for repeat
orders.
Damage to the leak tray or front cover possible while
carrying, setting up and installing a device. Grip the
device at its sides near the middle when lifting or mov-
ing.
Electrostatic-discharge hazard
Symbol Meaning

ASM 2.1L user manual V6815
Unpacking and setup 14
Prerequisite Check packaging for damage caused during transportation. If necessary,
put forward any claim for damages to the carrier.
Tools Utility knife
Bruising danger
Damage to the device by carrying or lifting it on protruding housing parts.
The device may fall and thus cause injuries.
Lift the device only centrally on the side of the housing.
Process 1. Set up the package in such a way that you can read the label. Using the
utility knife, cut the adhesive tape and open the packaging.
2. Remove the foam insert. Take out the accessory kit and the manual.
3. Open the accessory kit and check the scope of delivery. In case any
parts are missing, contact the Technical Support.
4. Clasp the device from below, lift it out of the packaging and place it on
its feet. Do not hold onto the front cover.
5. Check the device for signs of damage that occurred during transport.
In case you notice any damage, contact the Technical Support.
6. Place the device in its site of operation and remove protective foil.
Next steps Store packaging and keep the included packing list for repeat orders.
Connectors on the rear side
Device defect
Changes of the environmental temperature cause condensation inside
the device.
Allow device to acclimate for 3 h, before connecting to power supply
and taking into operation.
External devices like computers, fraction collectors, etc. can be connected
in 2 different ways to the detector:
Control with pin header
Connected to LAN within a network
Controlling with the pin header
Electronic defect
Connecting cables to the multi-pin connector of a switched on device
causes a short circuit.
Turn off the device before connecting cables.
Pull the power plug.
Connector Assignments The single ports exchange start, control and error signals with other
devices. Events can only be controlled by software.
Legend
1Label with serial
number
2LAN port
3Interface for the
Technical Support
4Pin header
5Fan
6Integrator output
7Connection and
power switch
Fig. 5 ASM 2.1L rear view
234 567
1

ASM 2.1L user manual V6815
15 Unpacking and setup
Connection Function
EV 3 (Event 3) Relay contact
The contact is on a floating basis. Its setting
depends on the settings in the software.
Steady-rate signal:
passive = open relay contact
active = closed relay contact
Pulse:
Closed relay contact for at least 1000 ms
Permissible load of the relay contact: 1 A/ 24 V DC
EV 2 (Event 2) OC output limited to 5 V
Levels:
passive 5 V
active 0 V
Pulse:
0 V for at least 1000 ms
EV 1 (Event 1) OC output limited to 5 V
Levels:
passive 5 V
active 0 V
Pulse:
0 V for at least 1000 ms
START IN TTL input
Low active
Secure switching threshold at least 1000 ms
After receiving a signal (short-circuit to ground) from
an external device, the device starts. If controlled with
software, an electronic trigger is send through the
LAN.
ERROR IN TTL input
Low active
Secure switching threshold at least 10 mA
After receiving a signal (short-circuit to ground) from
an external device, an error message appears and the
device stops.
ERROR OUT OC output limited to 5 V
Levels:
passive 5 V
active 0 V
Pulse:
0 V for at least 1000 ms
+5 V Provides a voltage of 5 V with respect to GND. This
makes it possible to supply a consumer that is
switched by an EVENT.
Max. current: 50 mA
GND Reference point of the voltage at the signal inputs

ASM 2.1L user manual V6815
Initial startup 16
Integrator output
The integrator output sends measuring signals from the detector.
non-bipolar
1 channel
0–5 V
DAC 16 bit
Scalable
Adjustable to offset
Initial startup
Optional mounting brackets, a holder for the Mobile Control or AZURA®
Click can be attached to ASM 2.1L during initial startup (see “Repeat
orders” on page 28). Please check the user manuals of the micro devices
to find all information on initital startup (see “Device overview” on page 5).
PEEK fittings
Component defect
Damage to the ports caused by strongly tightened fittings.
Use 5 Nm torque for stainless steel fittings.
Use 0.5 Nm torque for PEEK fittings.
ASM 2.1L as Compact HPLC isocratic
Tools Tool kit A1033
+24 V Event-controlled switching of 24 V
against GND
Max. current: 200 mA
GND Reference point of the voltage at the signal inputs
Connection Function
Fig. 6 Scheme of an isocratic compact HPLC system
Procedure Process
1. Connect the solvent bottles with degasser.
2. Connect the degasser with pump.
3. Connect the pump to the manual valve.
4. Connect the valve with column.
5. Connect the column to the detector.
6. Connect the detector to the waste bottle.

ASM 2.1L user manual V6815
17 Initial startup
Leak management
The leak management consists of the leak sensor and the drainage system
(funnels, hoses, nozzles). The drainage system ensures that escaping liq-
uids flow into a waste bottle. When leaks are registered by the leak sen-
sor, the LED flashes red. Both the device and the data acquisition via
chromatography software are stopped.
Prerequisite The front cover has been removed.
Next steps Attach the front cover.
Process Process Figure
1. Carefully push the funnel 1
into the center opening of the
capillary guide 2.
Fig. 7 Funnel and capillary guide
2. Push the long ending of the first
nozzle 4into the hose 3.
Fig. 8 Hose and nozzle
3. Connect the nozzle and the fun-
nel.
4. Push the other end of the hose
onto the nozzle 5of the leak
tray.
Fig. 9 Hose connected to device
5. For the bottom device, push
the short end of the nozzle 6
into the opening in the collec-
tion point of the leak tray.
6. Connect the hose to the nozzle
and lead the second ending to
the waste bottle.
7. Place the waste bottle below
the bottom device.
Fig. 10 Leak tray with nozzle
12
3
4
5
6

ASM 2.1L user manual V6815
Initial startup 18
Connecting a device to a computer to a
Local Area Network (LAN)
Note: HPLC devices made by KNAUER work only with IP adresses which
are assigned via IPv4. IPv6 is not supported.
This section describes how to set up an HPLC system in a local area net-
work (LAN) and how a network administrator can integrate this LAN into
your company network. The description applies to the operating system
Windows and all conventional routers.
To set up a LAN, we recommend to use a router. That means the following
steps are required:
Process 1. On the computer, go to the control panel and check the LAN proper-
ties.
2. Hook up the router to the devices and the computer.
3. On the computer, configure the router to set up the network.
4. Install the chromatography software from the data storage device.
5. Switch on the device and run the chromatography software.
Configuring the LAN Settings
The LAN uses only one server (which is normally the router) from that the
devices automatically receive their IP address.
Prerequisite In Windows, power saving, hibernation, standby, and screen saver
must be deactived.
In case you use an USB-to-COM box, the option "Allow the computer to
turn off ths device to save power" in the devicemanager must be deac-
tivated for all USB hosts.
For all LAN devices: For the network adapter, the following option in
the Device Manager must be deactivated: "Allow the computer to turn
off this device to save power".
Process 1. In Windows choose Start
 Control Panel Network and Sharing Cen-
ter
.
2. Double-click on
LAN Connection .
3. Click on the button Properties.
4. Select Internet Protocol version 4 (TCP/IPv4).
5. Click on the button Properties.
6. Check the settings in the tab General. The correct settings for the DHCP
client are:
a) Obtain IP address automatically
b) Obtain DNS server address automatically
7. Click on the button
OK .
Connecting the Cables
A router 3has several LAN ports 2and one WAN port 4that can be
used to integrate the LAN into a wide area network (WAN), e.g. a com-
pany network or the Internet. In contrast, the LAN ports serve to set up a
network from devices 1and a computer 5. To avoid interference, we
recommend operating the HPLC system separately from the company
network.

ASM 2.1L user manual V6815
19 Initial startup
You will find patch cables for each device and the router in the accessories
kit. To connect the router to a WAN, an additional patch cable is required,
which is not supplied within the scope of delivery.
Prerequisite The computer has been switched off.
There is a patch cable for each device and the computer.
Process 1. Use the patch cable to connect the router and the computer. Repeat
this step to connect all devices.
2. Use the power supply to connect the router to the mains power system.
Configuring the Router
The router is preset at the factory. You will find a label at the bottom side
of the router, on which IP address, user name, and password are printed.
These information help to open the router configuration.
Process 1. To open the router configuration, start your Internet browser and enter
the IP address (not for all routers).
2. Enter user name and password.
3. Configure the router as DHCP server.
4. In the router configuration, check the IP address range and make
changes if necessary.
Result Once the router has assigned IP addresses to all devices, the chromato-
graphy software can be used to remotely control the system.
Integrating the LAN into a Company Network
A network administrator can integrate the LAN into your company net-
work. In this case you use the WAN port of the router.
Prerequisite There is a patch cable for the connection.
Process 1. Check that the IP address range of the router and of the company net-
work do not overlap.
2. In case of an overlap, change the IP address range of the router.
3. Use the patch cable to connect the router WAN port to the company
network.
4. Restart all devices, including the computer.
Controlling Several Systems Separately in a LAN
Devices connected to a LAN communicate through ports, which are part
of the IP address. If more than one HPLC system is connected to the same
LAN and you plan on controlling them separately, you can use different
ports to avoid interference. Therefore, the port number for each device
must be changed and this same number must be entered into the device
configuration of the chromatography software. We recommend to use the
same port number for all devices in the same system.
Note: The port is set to 10001 at the factory. You must use the same num-
bers in the device configuration of the chromatography software as in the
device, otherwise the connection fails.
Process 1. Find out port number and change it on the device.
12
345

ASM 2.1L user manual V6815
Operation 20
2. Enter the port number in the chromatography software.
Result The connection is established.
Operation
A device can be operated in different ways:
with chromatography software
with Mobile Control
Chromatography Soft-
ware
To control the device with chromatography software, it must be con-
nected to the computer through the LAN interface. The list of supported
documents is public in the software section on the KNAUER website:
http://www.knauer.net/fileadmin/user_upload/produkte/files/Dokumente/
software/supported_instruments.pdf
You will find a detailed description on the chromatography software in the
software manual.
Mobile Control The Mobile Control is a program for a computer or tablet. To operate via
Mobile Control, the computer or tablet needs to be connected to a WLAN
router. You will find a detailed description in the Mobile Control user
manual V6851A: http://www.knauer.net/fileadmin/user_upload/produkte/
files/Dokumente/software/mobile_control/V6851A_Mobile-Control_User-
Manual_EN.pdf
Meaning of the LEDs
On the LED panel there are three LEDs 1and a standby button 2. The
figure shows the LED panel when the device is switched off.
The LEDs can have different colors depending on the operating condi-
tions.
12
Color Operating condition Operation
Left LED red flashing Error status Check the system.
Shortly press the
switch to deactivate
the error message.
red Fatal error Restart the device.
If the fatal error status
remains, contact Ser-
vice.
green Program or sequence
is running/was
loaded.
Center LED does not light Not ready