Thermo Scientific Evolution One Series User manual

Evolution Series UV-Visible Instruments
Evolution One Series
Spectrophotometer
User Guide
269-350500 Revision A October 2021

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The contents of this document are subject to change without notice. All technical information in this
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all previous information received by the purchaser.
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free and assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might
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Sale shall govern all conflicting information between the two documents.
For Research Use Only. This instrument or accessory is not a medical device and is not
intended to be used for the prevention, diagnosis, treatment or cure of disease.
WARNING Avoid an explosion or fire hazard. This instrument or accessory is not
designed for use in an explosive atmosphere.

Thermo Scientific Evolution One Series Spectrophotometer User Guide iii
C
Chapter 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
About the Hardware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Setting Up the Instrument. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Before Using the Spectrophotometer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
About This Document . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Organization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
E-mailing Data Files . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Product Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Warranty Period . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Items Not Covered by Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Warranty Repair . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Shipping Cost for Items Covered by Warranty . . . . . . . . . . . . . . . . . . . . . . . . 6
Update Policy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Trademarks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Site Preparation and Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Chapter 2 Evolution One Series Spectrophotometers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Safety Considerations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Safety Label Locations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Lifting or Moving the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Operating Precautions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Spectrophotometer Basics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Spectrophotometer Components. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Connectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Removable Panels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Corrosion Protection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Installing and Removing Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Standard Cell Holders . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Installing a Manual Accessory . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Installing a Smart Accessory. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Installing a Liquid Thermostatted Accessory . . . . . . . . . . . . . . . . . . . . . . . . . 20
Installing a Reference Cell Holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Contents

Contents
iv Evolution One Series Spectrophotometer User Guide Thermo Scientific
Using the Spectrophotometer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Computer Control Instruments. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Routine Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Cleaning the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Removing and Installing the Detector Module . . . . . . . . . . . . . . . . . . . . . . . 25
Ordering Parts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

Contents
Thermo Scientific Evolution One Series Spectrophotometer User Guide v

Thermo Scientific Evolution One Series Spectrophotometer User Guide 1
1
Introduction
This document covers the Thermo Scientific™ Evolution™ One series UV-Visible
spectrophotometers and accessories
About the Hardware
Contents
•Overview
•Setting Up the Instrument
•Before Using the Spectrophotometer
•About This Document
•Contacting Us
•E-mailing Data Files
•Product Warranty
•Trademarks
•Site Preparation and Safety
Contents
•Overview
•Setting Up the Instrument
•Before Using the Spectrophotometer

1Introduction
About This Document
2 Evolution One Series Spectrophotometer User Guide Thermo Scientific
Overview
The Evolution One series UV-visible spectrophotometers integrate advanced hardware
features with the power and flexibility of a wide range of Smart Accessories™. The One series
spectrophotometers include:
•EvolutionOne
•EvolutionOnePlus
All instruments include our Insight Pro software for data collection and analysis. The
Insight Pro software provides five modes of operation:
•Fixed, to measure the light passing through the sample at one or more wavelengths.
•Scan, to measure the light that passes through the sample over a range of wavelengths.
•Quant, to set up and perform quantitative analyses of sample data.
•Kinetics, to make time- and temperature-based kinetics measurements.
•Live Display, for quick measurements and simplified data collections in Fixed or
Scan mode.
Setting Up the Instrument
The spectrophotometer can be run from a Windows®-compatible computer connected to the
instrument (Computer Control). Computer Control instruments must be connected to an
external computer with the proper software installed. For instructions to set up a Computer
Control spectrophotometer, see Using the Spectrophotometer.
Before Using the Spectrophotometer
The spectrophotometer contains precise optical components. Handle it carefully. Before using
the system, review the Site Preparation and Safety information and Operating Precautions.
About This Document
Contents
•Organization
•Conventions

1 Introduction
About This Document
Thermo Scientific Evolution One Series Spectrophotometer User Guide 3
Organization
This document has the following main sections:
Conventions
This document uses the following conventions:
Section Description
Introduction Overview of the instrument and this document plus
information about registering your instrument, contacting us,
and the system warranty.
Evolution One Series
Spectrophotometers
Complete instructions for using and maintaining the
instrument.
Accessories Complete instructions for using accessories with the instrument.
WARNING Indicates a hazardous situation which, if not avoided, could result in death or
serious injury.
CAUTION Indicates a hazardous situation which, if not avoided, could result in minor or
moderate injury.
NOTICE Follow instructions with this label to avoid damaging the system hardware or
losing data.
Note Contains helpful supplementary information.
Tip Provides helpful information that can make a task easier.

1Introduction
Contacting Us
4 Evolution One Series Spectrophotometer User Guide Thermo Scientific
Contacting Us
For U.S. Technical Support, please contact:
Thermo Fisher Scientific
5225 Verona Road
Madison, WI 53711-4495 U.S.A.
Telephone: [phone]
E-mail: [e-mail]
For International Support, please contact:
Thermo Fisher Scientific
Telephone: [phone]
E-mail: [e-mail]
E-mailing Data Files
If you have questions about your data, you can send it to us as an archived workbook file
(*.iwbk). Please send us the appropriate file as an e-mail attachment.
Product Warranty
Warranties herein are for products manufactured by Thermo Fisher Scientific or its
authorized dealers.
Thermo Fisher Scientific warrants that this product is free from defects in labor and materials
and shall conform to its product specifications as defined in the product user documentation.
This warranty covers parts (except those specified below) and labor, and applies only to
equipment which has been installed and operated in accordance with the documentation
supplied by Thermo Fisher Scientific, and which has been serviced only by authorized
Thermo Fisher Scientific dealers or service personnel. This warranty does not apply to
equipment and accessories that have been modified or tampered with in any way, misused, or
damaged by accident, neglect, or conditions beyond Thermo Fisher Scientific's control.
Note Please have the instrument serial number available when you contact us.
Note Please do not send exported report files or user-defined workbooks-these do not
contain the information we need.