Thermo Scientific Evolution One Series User manual

Evolution Series UV-Visible Instruments
Evolution One Series
Spectrophotometer
User Guide
269-350500 Revision A October 2021

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The contents of this document are subject to change without notice. All technical information in this
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all previous information received by the purchaser.
Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or error-
free and assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might
result from any use of this document, even if the information in the document is followed properly.
This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This
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Sale shall govern all conflicting information between the two documents.
For Research Use Only. This instrument or accessory is not a medical device and is not
intended to be used for the prevention, diagnosis, treatment or cure of disease.
WARNING Avoid an explosion or fire hazard. This instrument or accessory is not
designed for use in an explosive atmosphere.

Thermo Scientific Evolution One Series Spectrophotometer User Guide iii
C
Chapter 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
About the Hardware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Setting Up the Instrument. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Before Using the Spectrophotometer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
About This Document . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Organization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
E-mailing Data Files . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Product Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Warranty Period . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Items Not Covered by Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Warranty Repair . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Shipping Cost for Items Covered by Warranty . . . . . . . . . . . . . . . . . . . . . . . . 6
Update Policy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Trademarks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Site Preparation and Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Chapter 2 Evolution One Series Spectrophotometers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Safety Considerations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Safety Label Locations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Lifting or Moving the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Operating Precautions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Spectrophotometer Basics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Spectrophotometer Components. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Connectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Removable Panels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Corrosion Protection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Installing and Removing Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Standard Cell Holders . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Installing a Manual Accessory . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Installing a Smart Accessory. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Installing a Liquid Thermostatted Accessory . . . . . . . . . . . . . . . . . . . . . . . . . 20
Installing a Reference Cell Holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Contents

Contents
iv Evolution One Series Spectrophotometer User Guide Thermo Scientific
Using the Spectrophotometer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Computer Control Instruments. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Routine Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Cleaning the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Removing and Installing the Detector Module . . . . . . . . . . . . . . . . . . . . . . . 25
Ordering Parts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

Contents
Thermo Scientific Evolution One Series Spectrophotometer User Guide v

Thermo Scientific Evolution One Series Spectrophotometer User Guide 1
1
Introduction
This document covers the Thermo Scientific™ Evolution™ One series UV-Visible
spectrophotometers and accessories
About the Hardware
Contents
•Overview
•Setting Up the Instrument
•Before Using the Spectrophotometer
•About This Document
•Contacting Us
•E-mailing Data Files
•Product Warranty
•Trademarks
•Site Preparation and Safety
Contents
•Overview
•Setting Up the Instrument
•Before Using the Spectrophotometer

1Introduction
About This Document
2 Evolution One Series Spectrophotometer User Guide Thermo Scientific
Overview
The Evolution One series UV-visible spectrophotometers integrate advanced hardware
features with the power and flexibility of a wide range of Smart Accessories™. The One series
spectrophotometers include:
•EvolutionOne
•EvolutionOnePlus
All instruments include our Insight Pro software for data collection and analysis. The
Insight Pro software provides five modes of operation:
•Fixed, to measure the light passing through the sample at one or more wavelengths.
•Scan, to measure the light that passes through the sample over a range of wavelengths.
•Quant, to set up and perform quantitative analyses of sample data.
•Kinetics, to make time- and temperature-based kinetics measurements.
•Live Display, for quick measurements and simplified data collections in Fixed or
Scan mode.
Setting Up the Instrument
The spectrophotometer can be run from a Windows®-compatible computer connected to the
instrument (Computer Control). Computer Control instruments must be connected to an
external computer with the proper software installed. For instructions to set up a Computer
Control spectrophotometer, see Using the Spectrophotometer.
Before Using the Spectrophotometer
The spectrophotometer contains precise optical components. Handle it carefully. Before using
the system, review the Site Preparation and Safety information and Operating Precautions.
About This Document
Contents
•Organization
•Conventions

1 Introduction
About This Document
Thermo Scientific Evolution One Series Spectrophotometer User Guide 3
Organization
This document has the following main sections:
Conventions
This document uses the following conventions:
Section Description
Introduction Overview of the instrument and this document plus
information about registering your instrument, contacting us,
and the system warranty.
Evolution One Series
Spectrophotometers
Complete instructions for using and maintaining the
instrument.
Accessories Complete instructions for using accessories with the instrument.
WARNING Indicates a hazardous situation which, if not avoided, could result in death or
serious injury.
CAUTION Indicates a hazardous situation which, if not avoided, could result in minor or
moderate injury.
NOTICE Follow instructions with this label to avoid damaging the system hardware or
losing data.
Note Contains helpful supplementary information.
Tip Provides helpful information that can make a task easier.

1Introduction
Contacting Us
4 Evolution One Series Spectrophotometer User Guide Thermo Scientific
Contacting Us
For U.S. Technical Support, please contact:
Thermo Fisher Scientific
5225 Verona Road
Madison, WI 53711-4495 U.S.A.
Telephone: [phone]
E-mail: [e-mail]
For International Support, please contact:
Thermo Fisher Scientific
Telephone: [phone]
E-mail: [e-mail]
E-mailing Data Files
If you have questions about your data, you can send it to us as an archived workbook file
(*.iwbk). Please send us the appropriate file as an e-mail attachment.
Product Warranty
Warranties herein are for products manufactured by Thermo Fisher Scientific or its
authorized dealers.
Thermo Fisher Scientific warrants that this product is free from defects in labor and materials
and shall conform to its product specifications as defined in the product user documentation.
This warranty covers parts (except those specified below) and labor, and applies only to
equipment which has been installed and operated in accordance with the documentation
supplied by Thermo Fisher Scientific, and which has been serviced only by authorized
Thermo Fisher Scientific dealers or service personnel. This warranty does not apply to
equipment and accessories that have been modified or tampered with in any way, misused, or
damaged by accident, neglect, or conditions beyond Thermo Fisher Scientific's control.
Note Please have the instrument serial number available when you contact us.
Note Please do not send exported report files or user-defined workbooks-these do not
contain the information we need.

1 Introduction
Product Warranty
Thermo Scientific Evolution One Series Spectrophotometer User Guide 5
Related Topics
Warranty Period
Items Not Covered by Warranty
Warranty Repair
Shipping Cost for Items Covered by Warranty
Update Policy
Warranty Period
With the exception of SPECTRONIC™ 20+, SPECTRONIC 20D+, GENESYS™ 20 and the
Helios™ Epsilon spectrophotometers, all UV Visible spectrophotometers come with a
standard warranty that is fourteen months from the date of shipment or twelve months from
the date of installation (whichever occurs first). The replacement parts and spare components
are warranted for 90 days from the date of shipment. If such parts are intended for long term
storage, it is recommended that their functionality is tested immediately upon receipt and any
problems reported to Thermo Fisher Scientific within the above specified warranty period.
The following warranties apply to instrument light sources:
1. Xenon lamps are warranted against failure for a period of three (3) years.
2. Tungsten and deuterium lamps will be replaced under warranty if they fail on the initial
power-up or within the first month of operation.
Thermo Fisher Scientific reserves the right to request the return of failed components that are
being replaced under warranty.
User should complete and return the Product Registration card, and retain proof of
delivery date.
Items Not Covered by Warranty
This warranty does not apply to glassware, expendable components, peripheral devices or
accessories not manufactured by Thermo Fisher Scientific. The manufacturer of these
products may offer specific warranties for such parts and components.
Cleaning and calibration service is not covered by this warranty.

1Introduction
Product Warranty
6 Evolution One Series Spectrophotometer User Guide Thermo Scientific
Warranty Repair
In the event of failure within the warranty period, Thermo Fisher Scientific will at Thermo
Fisher Scientific's option, repair or replace the product not conforming to this warranty.
There may be additional charges, including freight, for warranty service performed in some
countries. For service, call Thermo Fisher Scientific (or its authorized dealer outside the
United States and Canada). Thermo Fisher Scientific reserves the right to ask for proof of
delivery date.
Warranty repairs for UV Visible instruments may vary per product line and region. UV
Visible products manufactured by Thermo Fisher Scientific are covered by a
"return-to-factory" parts and labor warranty. In some regions, on-site warranties, extended
warranties and service contracts may be purchased for all products.
In the event that on-site warranty service is required, and no on-site service contract is active
on this product, the customer is responsible for any travel expenses, labor costs, and additional
expenses which may be incurred in the performance of on-site warranty service.
Please contact your dealer or Thermo Fisher Scientific Technical Service department for
further information. A Return Authorization Number must be obtained from Thermo Fisher
Scientific Technical Service before returning any product for in-warranty repair
or replacement.
Shipping Cost for Items Covered by Warranty
It is customer's responsibility to pay for the shipment of the product under warranty to
Thermo Fisher Scientific.
This warranty covers return shipment (standard ground) charges for returning the repaired or
replaced product to the customer.
Update Policy
Thermo Fisher Scientific may, from time to time, revise the performance of its products, and
in doing so incur no obligation to furnish any such revisions to any Thermo Fisher Scientific
customer.
THIS WARRANTY IS IN LIEU OF ALL WARRANTIES EXPRESSED, IMPLIED, OR
STATUTORY, INCLUDING, BUT NOT LIMITED TO, WARRANTIES OF FITNESS
FOR A PARTICULAR PURPOSE OR MERCHANTABILITY OR OTHERWISE, and
states Thermo Fisher Scientific's entire and exclusive liability and the Customer's exclusive
remedy for any claim in connection with the sale or furnishing of services, goods, or parts,
their design, suitability for use, installation, or operations. Thermo Fisher Scientific will in no
event be liable for any direct, indirect, special, or consequential damages, whatsoever,
including loss of goodwill, whether grounded in tort (including negligence), strict liability or
contract, and Thermo Fisher Scientific's liability under no circumstances will exceed the
contract price for the goods and/or services for which liability is claimed.

1 Introduction
Trademarks
Thermo Scientific Evolution One Series Spectrophotometer User Guide 7
REPRESENTATION AND WARRANTIES MADE BY ANY PERSON, INCLUDING
ITS AUTHORIZED DEALERS, REPRESENTATIVES AND EMPLOYEES OF
THERMO FISHER SCIENTIFIC WHICH ALTER OR ARE IN ADDITION TO THE
TERMS OF THIS WARRANTY SHALL NOT BE BINDING UPON THERMO
FISHER SCIENTIFIC UNLESS IN WRITING AND SIGNED BY ONE OF ITS
OFFICERS.
Trademarks
Microsoft, Windows and Excel are either trademarks or registered trademarks of Microsoft
Corporation in the United States and/or other countries.
Teflon, Viton and Freon are either trademarks or registered trademarks of E. I. du Pont de
Nemours and Company (DuPont) or its affiliates in the United States and/or other countries.
Starna is either a trademark or a registered trademark of Starna Scientific Limited in the
United States and/or other countries.
Hellma is either a trademark or a registered trademark of Hellma GmbH in the United States
and/or other countries.
AccuVac and Hach are either trademarks or registered trademarks of Hach Company in the
United States and/or other countries.
Spectralon is either a trademark or a registered trademark of Labsphere, Inc. in the United
States and/or other countries.
All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.
Site Preparation and Safety
Before using the system, read the site preparation and safety manual on the provided
documentation CD. Always follow the safety precautions in that manual and in this
document when using the system

1Introduction
Site Preparation and Safety
8 Evolution One Series Spectrophotometer User Guide Thermo Scientific

Thermo Scientific Evolution One Series Spectrophotometer User Guide 1
2
Evolution One Series Spectrophotometers
The Evolution™ One series UV-Visible spectrophotometers includes two models:
•EvolutionOne
•EvolutionOnePlus
Safety Considerations
Each person using the spectrophotometer should read the general safety information in the
Site and Safety Information guide (see the documentation CD) and the instrument-specific
safety information provided here.
Contents
•Safety Considerations
•Operating Precautions
•Spectrophotometer Basics
•Installing and Removing Accessories
•Using the Spectrophotometer
•Maintenance
•Ordering Parts
Contents
•Safety Label Locations
•Lifting or Moving the Instrument
WARNING Do not operate this system without following the safety precautions described
in this manual and the documentation that came with your system.

2Evolution One Series Spectrophotometers
Safety Considerations
2 Evolution One Series Spectrophotometer User Guide Thermo Scientific
Safety Label Locations
This section shows the locations of safety labels on the spectrophotometer. If there are
questions or problems with the safety labels, contact us using the information provided at the
beginning of this document.
Back view
Right side view
Compliance label
UV radiation warning label
(under mercury lamp access cover)

2 Evolution One Series Spectrophotometers
Safety Considerations
Thermo Scientific Evolution One Series Spectrophotometer User Guide 3
Left side view
Related Topics
Removing and Installing the Detector Module
Lifting or Moving the Instrument
To avoid risk of injury, use proper techniques when lifting or moving the instrument or other
system components.
UV radiation warning label
(under detector access cover)
Hand hold
Hand hold

2Evolution One Series Spectrophotometers
Operating Precautions
4 Evolution One Series Spectrophotometer User Guide Thermo Scientific
Operating Precautions
The spectrophotometer contains precise optical components. Handle it carefully and follow
these precautions:
• Do not allow moisture to leak into the instrument interior.
• Wipe off spilled chemicals immediately.
• Do not drop the instrument.
• Protect the instrument from mechanical shock.
• Protect the instrument from dust.
Spectrophotometer Basics
Contents
•Spectrophotometer Components
•Connectors
•Removable Panels
•Corrosion Protection

2 Evolution One Series Spectrophotometers
Spectrophotometer Basics
Thermo Scientific Evolution One Series Spectrophotometer User Guide 5
Spectrophotometer Components
The following illustration identifies major components visible on the outside of a typical
spectrophotometer. (Some components may not be present on all instruments.)
Spectrophotometer Z-height
The z-height (distance from the bottom of the cell to the center of the light beam) for the
spectrophotometer is 8.5 mm.
Related Topics
Opening and Closing the Sample and Reference Compartment Doors
Computer Control
Insight Pro Software
Opening and Closing the Sample and Reference Compartment Doors
To open the sample compartment, press the button on its door. To close the compartment,
slide the door forward until it latches.
To access the reference compartment, lift or lower the door.
Related Topics
Sample Compartment Front Panel
Tubing Access Panel
Sample compartment Reference compartment

2Evolution One Series Spectrophotometers
Spectrophotometer Basics
6 Evolution One Series Spectrophotometer User Guide Thermo Scientific
Computer Control
The spectrophotometer can be run from a Windows®-compatible computer connected to the
instrument (Computer Control). Computer Control instruments must be connected to an
external computer with the proper software installed.
Insight Pro Software
All instruments include our Insight Pro™ software for data collection and analysis. See the
Insight Pro Software User Guide.
Connectors
This section shows the locations of the connectors inside and outside the spectrophotometer.
Outside Cover
This illustration shows the connectors on the instrument outside cover.
Back view
Contents
•Outside Cover
•Inside the Sample Compartment
Computer
Trigger Power
Power switch
Hg lamp
Purge