VWR avantor CHEMI User manual

Version 01 Issue 04/2021
Instruction Manual
VWRGel Documentation System CHEMI Only
European Catalogue Number: 730-1471

2 vwr.com Instruction manual VWR® CHEMI Only
Legal Address of Manufacturer
Europe
VWR International bv
Researchpark Haasrode 2020
Geldenaaksebaan 464
B-3001 Leuven
[phone]
http://be.vwr.com
UK Importer:
VWR International Ltd
Hunter Boulevard, Magna Park
Lutterworth, Leicestershire, LE17 4XN
http://uk.vwr.com
Country of Origin
UK.

3 vwr.com Instruction manual VWR® CHEMI Only

3 vwr.com Instruction manual VWR® CHEMI Only
Legal Address of Manufacturer
2
Safety Instructions
6
Unpacking a Chemi Only System
9
Removing from the Packaging
9
Visual Inspection
9
Unpacking the Main Box
9
Overview
12
Chemi Only System
12
Gel Doc image capture Software
12
Software Installation
14
Minimum System Requirements
14
Software Installation
14
Run the Set-up Program
14
Select the Instrument Hardware Information
14
Select if running Gel Doc in a CFR21 part 11 Compliance environment
15
Select the Installation Folder Destination Location
15
Select the Start Menu Folder
16
Install Device Drivers using the Device Driver Installation Wizard
16
Operating
18
Operator Controls
18
Rear Panel
18
Connected PC
18
Instrument Set-up - Darkroom
18
Entering Personal Preferences
21
Adjusting Basic Screen Viewing Size
28
Loading Test Samples
28
Sample Drawer
28
Automatic Image Capture Mode
29
Automatic Image Capture Chemi Rapid
29
Automatic Image Capture Chemi Blot (single image)
29
Automatic Image Capture Chemi Blot (series)
30
Automatic Image Capture- Signal Accumulation Calculator (SAC)
30
Automatic Image Capture - Sample Positioning and capturing an image
31
Automatic Image Capture - Image Actions Following Capture
36
Manual Image Capture Mode
36
Manual Image Capture - Start
36
Manual Image Capture - Single Image
37
Manual Image Capture - Series
43
Manual Image Capture - Additive Series
47
Manual Image Capture - Multiplex
50
Manual Image Capture - Image Actions Following Capture
54
Protocols - Saving, Opening and Editing
55
Introduction
55
Creating and Saving a Protocol
55
From the Home Screen
55
In Auto Image Capture Mode
56
In Manual Image Capture Mode
57
Using Protocols
58
Opening a Protocol
58
Deleting a Protocol
59
Editing a Saved Protocol
60
Image Functions
61
Table of Contents

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Load Images Screen
61
Image Actions from Load Images Screen
62
View Images Screen
63
View Images Screen - Single Images
63
View Images Screen - Multiplexed Images
64
View Images Screen - Image Display Options
64
View Images Screen - Combining Single Images into a New Multiplexed Image
67
Image Edit Screen
68
Image Edit Screen - Image Editing Main Options
68
Image Edit Screen - General Option
69
Image Edit Screen - Annote Option
71
Image Edit Screen - Enhance Option
73
Image Edit Screen - General / Annotate / Enhance Options Common Control
75
Image Edit Screen - 3D View Option
77
Image Edit Screen - All Options Common Controls
79
Common Screen Functions and Tools
81
Saving Images
81
Printing
85
Operator Maintenance
90
Looking After a Chemi Only Instrument
90
Cleaning a Chemi Only Instrument
90
Contacting VWR International
90
Chemi Only Troubleshooting
92
Disposal
93
Disposing of a Chemi ONLY
93
The Waste Electrical and Electronic Equipment (WEEE) Directive
93

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Safety Instructions
Safety Practices
This document describes the general safety practices and precautions that must be observed when
operating a VWRGel documentation Chemi Only System.
This advice is intended to supplement, not supersede, the normal safety codes in the user’s country. The
information provided does not cover every safety procedure that should be followed. Ultimately,
maintenance of a safe laboratory environment is the responsibility of the user and the user’s organisation.
Please consult all documentation supplied with the VWRGel documentation Chemi Only System before
starting to work with the instrument. Carefully read the safety information in this document and in the other
documentation supplied. When setting up the instrument or performing analysis or maintenance
procedures, strictly follow the instructions provided.
Warning Notices
Within this User Guide WARNINGS are used to highlight information or instructions that must be followed
in order to avoid personal injury to yourself or other people in the vicinity, e.g. switch off the mains voltage
and remove the mains cord before cleaning.
WARNINGS appear as below:
WARNING
Switch off the mains voltage and remove the mains cord before cleaning.
WARNING
Ensure that all instrument users read and understand the precautions listed below.
WARNING
You are advised to post a copy of the precautions near to or on the instrument
itself.
Precautions
The following precautions must be observed when using a VWRChemi Only System:
Be sure that the voltage of the VWRChemi Only System corresponds to the voltage available
where it is to be installed.
Never remove the side or back panels of the VWRChemi Only System without first shutting
down the instrument and disconnecting the mains cord.
General Operating Conditions
VWRChemi Only Systems have been designed and tested in accordance with the safety requirements of
the International Electrotechnical Commission (IEC). VWRChemi Only Systems conform to IEC61010-
1:2010 (Safety Requirements for electrical equipment for measurement, control and laboratory use) as it
applies to IEC Class 1 (earthed) appliances, and therefore meet the requirements of EC directive
2006/95/EC.

6 vwr.com Instruction manual VWR® CHEMI Only
If possible, avoid any adjustment, maintenance or repair to the instrument while it is open and operative.
However, if any adjustment, maintenance or repair is necessary while the instrument is open, this must be
done by a skilled person who is aware of the hazards involved.
Whenever circumstances arise that mean a VWRChemi Only System may be unsafe, make it
inoperative. In particular, a VWRChemi Only System may be unsafe if it:
Shows visible damage.
Fails to perform the intended measurement.
Has been subjected to severe transport stresses.
Has been subjected to prolonged storage in unfavorable conditions.
Environmental Conditions
VWRChemi Only Systems should only be used under the following conditions:
Indoors.
At altitudes below 2000m.
In ambient temperatures between 5°C and 40°C.
With relative humidity below 80% for temperatures up to 31°C, decreasing linearly to 50%
relative humidity at 40°C.
Electrical supply fluctuations not exceeding +10% of the nominal voltage.
WARNING
The protection provided by the equipment may be impaired if the environmental
conditions do not lie within these parameters.

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Electrical Safety
VWRChemi Only Systems are designed to protect the user from potential electrical hazards. This section
describes some recommended electrical safety practices.
WARNING
Lethal voltages are present at certain points within the instrument.
When the instrument is connected to mains power, removing the instrument covers
is likely to expose live parts.
Even when the power switch is off, high voltages can still be present –capacitors
within the instrument may still be charged even if the instrument has been
disconnected from all live voltage sources.
The VWRChemi Only System must be correctly connected to a suitable electrical supply. The supply
must have a correctly installed protective conductor (earth or ground) and must be installed and checked
by a qualified electrician before connecting the instrument.
WARNING
Any interruption of the protective conductor inside or outside the VWRChemi
Only System, or disconnection of the protective conductor terminal is likely to
make the instrument dangerous.
Intentional interruption of the protective conductor is prohibited.
WARNING
Ensure that the mains supply socket on the instrument is not obstructed, i.e. leave
a gap to allow easy disconnection of the mains cord.
WARNING
If the mains power cord has to be replaced, ensure that the replacement cord is
appropriately rated and approved for the intended use.
When working with the VWRChemi Only System:
Connect the instrument to a correctly installed mains power outlet that has a protective conductor
connection.
Do not operate the instrument with any covers or internal parts removed.
Do not attempt to make internal adjustments or replacements except as directed in the VWR
Chemi Only System documentation.
Disconnect the instrument from all live voltage sources before opening it to make any
adjustments, replacements, maintenance or repair. If the opened instrument must be operated
for further adjustment, maintenance or repair, this must only be done by a supplier’s Service
Engineer.
If it is possible that the instrument is no longer electrically safe for use, make the instrument
inoperative and secure it against any unauthorised or unintentional operation. The electrical
safety of the instrument is likely to be impaired if:
It has any signs of visible damage.
If it has been subjected to prolonged storage in unfavourable conditions.
If it has been subjected to severe stress during transportation.
Electrical Protection
Insulation: Class I rating for external circuits. Only connect equipment that meets the
requirements of IEC 61010-1:2010, IEC 60950-1:2005 (including AMD1:2009 AND AMD2:2013
CSV) or equivalent standards.

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Installation Category: The instruments are able to withstand transient overvoltages typically
present on the mains supply. The normal level of transient overvoltages is impulse withstand
(overvoltage) category II of IEC 60664-1:2007.
Pollution Degree 2: Normally only non-conductive pollution occurs. Occasionally, however,
temporary conductivity caused by condensation must be expected.
EMC Compliance
EC Directive
The VWRChemi Only System is designed and tested to meet the requirements of the EC directive
2004/108/EC and 93/68/EC. The instrument complies with the EMC standard EN61326-1:2013 (EMC
standard for electrical equipment for measurement, control and laboratory use) and EN55011:2009 AND
A1:2010 (ISM) class A (rf emissions).
FCC Rules and Regulations
The VWRChemi Only System is classified as a digital device used exclusively as industrial, commercial
or medical test equipment. It is exempt from the technical standards specified in Part 15 of the FCC Rules
and Regulations based on Section 15.103 (c).
Warning Labels
Warning labels attached to the instrument draw attention to specific hazards - refer to this User Guide and
other documentation provided with the instrument for more details concerning potential hazards and any
precautions or other actions that must be taken.
WARNING
To prevent potential personal injury and damage to the instrument, switch OFF all
components in the system and disconnect them from the mains power supply
before altering, or making any new electrical connections.

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Unpacking a VWR® Chemi Only System
Removing from the Packaging
Visual Inspection
Upon taking delivery of a new VWRChemi Only Instrument:
Check the contents of the cartons as they are unpacked against the contents listed on the
packing list
Check each item on the packing list for damage and document any damage carefully
If any items are missing or damaged, contact your local distributor immediately
Unpacking the Main Box
The VWRChemi Only Instrument is packaged in a large carton which is securely strapped. Some
Instruments may be shipped as a carton only, others may be shipped with the carton strapped onto a
wooden pallet. Accessories are shipped in one or more separate cartons.
To unpack a typical VWRChemi Only Instrument:
WARNING
VWRChemi Only Instrument requires two people to lift it safely. A VWRChemi
Only Instrument complete weighs approximately 20Kg.

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VWRChemi Only Unpacking Guide
A. Original packing of VWRChemi only
B. Open the box and remove the
drawer and accessories bag
C. Remove the cardboard surrounding
the VWRChemi Only and gently
lift out of box
D. Remove the foam and plastic
surrounding the VWRChemi Only
E. Slide in the VWRChemi Only
drawer and the system is now ready
to use

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PACKAGE CONTENTS
Mains power cord - suitable for country of use
Camera to PC lead (USB Type A)
Darkroom to PC USB lead

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Overview
The VWRGel Documentation Chemi Only system is dedicated to Chemiluminescence imaging. The
system is built for high performance, automation and features a next generation high quantum
efficiency CCD camera for even greater sensitivity.
Western blot imaging has never been so easy than with the VWRChemi Only system. The system
automatically selects the right imaging conditions for any blot irrespective of the chemiluminescent
reagents being used.
Darkroom - this provides a completely black environment into which the sample to be imaged is
enclosed.
White LED lights are provided on each side of the Darkroom to provide general illumination when
positioning and setting up samples.
VWRChemi Only system
The VWRChemi Only system is to be used with your own monitor and computer.
Specifications
Image resolution (pixels M)
4
Effective resolution (pixels M)
16
A/D
16 Bit
Greyscales
65536
Quantum efficiency @425nm
73%
Cooling regulated (degrees)
-57oC
Lens (motor driven)
Fixed f0.95
Epi LED white lights
Yes
Maximum blot size
11x8cm
Use with external PC
Yes
Software
VWRGel Doc Image capture
software and VWRGel Doc
software
VWRImage Capture Software
The VWRImage Capture software runs in a standard Windows environment and provides an application
driven and time-saving workflow for running a variety of life-science applications.
The VWRChemi Only Instrument is controlled and operated by the VWRImage Capture software
running on an external PC operating a standard Microsoft Windows environment. Two cable connections
are required between the PC and the VWRChemi Only Instrument; one for the Darkroom, and one for the
Camera.
The raw data recorded by the digital camera is processed by the VWRImage Capture software to
generate the images and technical files which are the VWRChemi Only Instrument's basic outputs.

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The VWRImage Capture software provides both automatic and manual modes of control of the VWR
Chemi Only hardware and has an inbuilt database of application specific data which it uses in automatic
capture mode to optimise the hardware configuration in order to obtain the optimal sample image. The
database contains information relating to:
Sample format; Blot
Blot type; Chemiluminescence
Sample type; Protein
Substrate types
TYPICAL VWRCHEMI Only - FRONT
Darkroom
Sample drawer

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Software Installation
Minimum System Requirements
The VWRImage Capture software can operate on the following Microsoft Windows Operating Systems:
Windows 7 Professional SP3 (32 bit and 64 bit versions)
Windows 8 Professional
Windows 10 Professional and Windows 10 Enterprise
Note: Home versions of the Windows Operating Systems are not supported.
Software Installation
A VWRChemi Only Instrument needs to have the VWRImage Capture software installed on the
associated PC from which the instrument is to be controlled.
The VWRImage Capture software can only be installed from a Windows account that has Administrator
rights. Installation is performed in the following order:
1. Run the Set-up Program
2. Select the Instrument Hardware Information
3. Select the Installation Folder Destination Location
4. Select the Start Menu Folder
5. Install Device Drivers using the Device Driver Installation Wizard
Run the Set-up Program
1. Insert the VWRImage Capture USB stick/CD ROM and run the Set-Up Program. The
following screen will appear:
SETUP - VWRImage Capture - WELCOME TO THE VWRImage Capture SETUP WIZARD SCREEN
2. Click on the Next > button. The first Hardware Information screen will appear.
Select the instrument Hardware Information
SET-UP -VWRImage Capture- HARDWARE INFORMATION SCREEN-INSTRUMENT TYPE

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3. Select the instrument type that you have by selecting the appropriate radio button, i.e. VWR
CHEMI only.
4. Click on the Next > button. The CFR compliance screen will appear
Select if running VWRImage Capture software in a CFR21 part 11 compliance
environment
SET-UP-VWRIMAGE CAPTURE-CFR COMPLIANCE
5. Click on the Next > button. The setup-Select Destination Location screen will appear
Select the Installation Folder Destination Location
SET-UP VWRImage Capture-SELECT DESINATION LOCATION SCREEN
Select the folder in which you would like the VWRImage Capture to be installed:
To accept the displayed default folder click on the Next > button.
To choose a different folder click on the Browse... button and navigate to the desired folder or
create a new folder. Then click on the Next > button.
Note: The VWRImage Capture software installation requires free space of at least 80.7MB.
6. The Setup-VWRImage Capture Select Start Menu Folder screen will appear.

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Select the Start Menu Folder
SET-UP-VWRImage Capture-SELECT START MENU FOLDER SCREEN
7. Select the folder in which you would like VWRImage Capture program start shortcut to be
installed:
To accept the displayed default folder click on Next > button.
To choose a different folder click on the Browse... button and navigate to the desired
folder or create a new folder. Then click on the Next > button.
8. The Ready to Install screen will appear. Click on the install button to start the installation
process.
SETUP- VWRImage Capture –READY TO INSTALL SCREEN
9. The Device Driver Installation Wizard screen will appear
Install device drivers using the Device Driver Installation Wizard
This part of the installation process installs the software drivers that some computers need in order to
complete the installation.

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DEVICE DRIVER INSTALLATION WIZARD WELCOME SCREEN
10. Click on the Next > button. The Wizard (a sub-program, part of the VWRImage Capture
software) will automatically install the necessary device drivers on the PC that the hardware
device you selected previously needs in order to operate.
Once the device drivers have been installed, the screen will change to the second Device Driver
Installation Wizard screen. This displays details of the device drivers that have been installed.
DEVICE DRIVER INSTALLATION WIZARD FINISH SCREEN
11. Click on the Finish button.
12. The setup-VWRImage Capture software completing the VWRImage Capture Setup Wizard
screen will appear. This indicates that the installation process has finished successfully.
SETUP-VWR Image Capture- COMPLETING THE VWRImage Capture SETUP WIZARD SCREEN
13. Click on the Finish button to complete the set-up process.
14. Remove the VWRImage Capture USB Stick.

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Operating
Operator Controls
Rear Panel
The rear panel of the VWRChemi Only Instrument contains two User controls:
An ON/OFF rocker switch, used for energising/de-energising the instrument.
Connected PC
The keyboard and mouse of an external PC connected to a VWRChemi Only Instrument function as
normal.
Instrument Set-up - Darkroom
Make sure that the Laboratory bench is strong enough to support the VWRChemi Only Instrument and is
stable and level.
Caution
Do not energise any of the components until instructed to.
Make the following cable connections:
15. Connect the mains power cord between a power supply socket and the Instrument. DO NOT
switch on.
WARNING
Ensure that the mains supply socket on the Instrument is not obstructed, i.e. leave
a gap to allow easy disconnection of the mains cord.
16. Connect Darkroom to the PC using the USB lead.
17. Connect the camera USB cable to the back of the darkroom to connect the camera to the PC.
18. Energise the Darkroom - ON/OFF switch on the back of the Darkroom.
19. Startup the PC and install the VWRImage Capture software.
20. The VWRGel Doc software will install automatically.
21. Double click the ( ) icon to start the VWRImage Capture software.

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VWRImage Capture HOME SCREEN
VWRImage Capture Standard View Home Screen Layout
TITLE BAR
Displays an icon to show where you are in the VWRImage Capture
program, e.g. here it shows the icon to show that you are on
the Home Screen. On other screens within the VWRImage Capture
program this button is used to return you to the Home screen.
Displays the camera resolution, the connected hardware and the
program title , for this particular installation it
shows that a with a VWRChemi only with a 4MP image resolution
system is installed and the VWRImage Capture program is running.
Displays the icon, for accessing System data - camera hardware
and program version information, as shown below:
WORKFLOW
STATUS BAR
INFORMATION
PANES
USER
SELECTABLE
ACTIONS
TITLE
BAR
NAVIGATION ICONS