WPI UltraPath 2 Parts list manual

SYSTEM GUIDE
for visible and ultraviolet (UPUV-2) systems
UltraPath 2
Multiple pathlength sample cell for absorbance
spectroscopy with extended dynamic range
Serial No._____________________
www.wpi-europe.com
180522

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CONTENTS
GENERAL WARNINGS AND CAUTIONS......................................................................................... 3
INTRODUCTION ........................................................................................................................... 4
INSTRUMENT DESCRIPTION ........................................................................................................ 5
UltraPath 2 Sample Cell..................................................................................................... 6
TIDAS S 300 UV/VIS Spectrometer .................................................................................... 7
Fiber Optic Cables ............................................................................................................. 8
Peri-Star Pro Peristalitic Pump .......................................................................................... 8
Cleaning Kit (Available US Only)........................................................................................ 9
LWCC Injection System...................................................................................................... 9
Setting up UltraPath 2 ............................................................................................................... 10
Parts List.......................................................................................................................... 10
Opening the package ...................................................................................................... 10
Assembling the UltraPath 2 system ................................................................................ 12
Installing the software .................................................................................................... 13
Setting up QFT2 Glass Fiber Filter (GF/F) & cuvette holder....................................................... 14
Using UltraPath 2....................................................................................................................... 15
How to fill the UltraPath 2 sample cell............................................................................ 15
Acquiring Data with TIDASDAQ –a typical measurement cycle...................................... 16
Refractive index sensitivity of the UltraPath 2 sample cell ............................................. 18
- salinity matched reference solutions to avoid baseline offsets .................................... 18
Measurement reliability.................................................................................................. 18
Flow rate and maximum pressure................................................................................... 18
Effective pathlength and linearity................................................................................... 19
Using the QFT2 cuvette holder for particulate absorption measurements............................... 20
Background ..................................................................................................................... 20
Taking particulate absorption measurements with a GF/F pad ...................................... 21
Taking cuvette measurements........................................................................................ 21
Relevant Literature for particulate absorption measurements ...................................... 22
Instrument Maintenance........................................................................................................... 23
Self-Test..................................................................................................................................... 25
Troubleshooting ........................................................................................................................ 26
Storage ...................................................................................................................................... 28
Accessories ................................................................................................................................ 29
Specifications............................................................................................................................. 30
References................................................................................................................................. 31
WARRANTY................................................................................................................................ 33
Copyright © 2022 by World Precision Instruments. All rights reserved. No part of this publication may be
reproduced or translated into any language, in any form, without prior written permission of World
Precision Instruments.

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GENERAL WARNINGS AND CAUTIONS
Read this manual before you attempt to use this instrument.
All warnings on the unit and in these operating instructions should be adhered to.
Warning: Do not look directly into the light output of the light source. light radiation may
damage your eyes.
Warning: Do not remove any safety devices installed. this will void your warranty and create
an unsafe operating condition.
Warning: Dangerous voltages are present. No user serviceable parts inside unit. Instrument
should be serviced by qualified service personnel only.
Warning: The UltraPath 2 sampling system contains cleaning solutions. only qualified
personnel should use these solutions. Please refer to the SDS Sheets for details on the
solutions.
Warning: Before using the instrument for the first time, check for transport damage.

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INTRODUCTION
The UltraPath 2 sampling system is a unique high-performance spectrophotometer offering
user-selectable optical path lengths of 10, 50 and 200 cm. Designed for the detection of low
absorbing species in solution, UltraPath 2 is an ideal tool for any study requiring precise and
highly sensitive spectroscopic determination of analytes, either in the lab or in the field. The
instrument operates in the wavelength range of 220 to 720 nm (UPUV-2) and has an
exceptional dynamic range allowing reliable absorbance measurements between 5μAU/cm to
1 AU/cm to be routinely made.
The UltraPath system was developed by WPI under a collaborative agreement with NASA
(Stennis Space Center) for the spectroscopic determination of colored dissolved organic matter
(CDOM) in seawater and freshwater environments. It was designed for use in the laboratory
and in the field (i.e., at sea). CDOM concentrations vary significantly between open ocean
samples with low CDOM (e.g., 0.007 m-1 at 380 nm), and high CDOM freshwater environments
(e.g., 10-20 m-1 at 380 nm).
To address these problems the design requirements of UltraPath mandated the development
of a rugged portable system capable of highly sensitive measurements across a wide dynamic
range.
The UltraPath 2 sample cell has three optical pathlengths contained within a single sample cell
(10 cm, 50 cm and 200 cm). The pathlengths are user selectable, offering a very high sensitivity
and an extended dynamic range for VIS and UV absorbance measurements. The fluid path of
the sample cell is optimized to produce a laminar flow that is virtually free of interference from
trapped air bubbles and adherence of dissolved substances to the cell wall. In particular, the
design greatly minimizes the problems commonly found with flow cells of long optical
pathlengths: the risk of trapping dust particles, fibers or particulate matter inside the cell.

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INSTRUMENT DESCRIPTION
The UltraPath 2 system includes a photodiode array-based spectrometer module (TIDAS S 300
UV/VIS) to measure the absorbance at the selected pathlength. Absorption of light is
measured between 190 to 720 nm with a resolution of 4 nm (FWHM) and a noise level below
0.2 mAU. The spectrometer includes an integrated deuterium/halogen light source which
delivers light via the supplied fiber optic cables to the UltraPath 2 sample cell. A peristaltic
pump (Peri-Star Pro) is utilized to evenly draw sample up into the Sample Injector and through
the UltraPath 2 sample cell.
The spectrophotometer is connected to the network via an ethernet port. The
TIDASDAQ/SpectraView software (included) is installed on a standard Windows PC or laptop
(not included) and connects to the TIDAS via the network. The TIDASDAQ/SpectraView
software displays and performs data collection and processing. High precision absorbance or
transmittance spectra can be obtained within seconds. Key features of the software package
include ease-of-use, simple spectra acquisition, as well as tools for sample qualification,
quantification, and data extraction. (Refer to the TIDASDAQ & SpectraView manuals on the
USB drive for a detailed description of the software supplied with the instrument.)

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UltraPath 2 Sample Cell
1. Fiber optic input connector
2. Liquid Input connector
3. Liquid Output Connector. Sample is removed from the UltraPath 2 sample cell via
the Liquid Output Connector with vacuum suction of the Peri-Star Pro peristaltic
pump.
4. Fiber Optic Switch & Output Connector. The optical pathlength of the UltraPath 2
can be switched between 10, 50 and 200 cm. Pull the knob and turn to the selected
pathlength
5. UltraPath 2 Sample Injector Kit. The Sample Injector (WPI #58006) is connected to
the Liquid Input connector (7). Sample is drawn into the UltraPath 2 sample cell with
the Sample Injector using vacuum suction of the Peri-Star Pro peristaltic pump.
6. Silicone pump tubing (WPI# 500320 –part of the sample injector assembly kit WPI#
58006).
7. Waste Bottle for expelled sample (included with WPI# 89372-2 LWCC Injection Kit).

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TIDAS S 300 UV/VIS Spectrometer
8. Status LED. LED’s indicating the TIDAS S 300 UV/VIS state.
9. Error LED Indicator (Red). The Error LED lights up when the TIDAS S 300 UV/VIS is in
a fault state. On system start-up the error LED is on for up to 3 seconds.
10. Front Power Switch (ON/OFF). When the power switch is turned ON, power is
supplied to the TIDAS S 300 UV/VIS spectrometer module and this LED will be lit.
11. Fiber optic input (SMA type). The Fiber optic input connects to the light output of
the UltraPath 2 sample cell (4).
12. Debug RS-232. Interface for system diagnostics (connectors supplied). For further
instructions refer to TIDAS S 300 UV/VIS instruction manual.
13. LAN RJ45 ethernet communication interface. The TIDAS S 300 UV/VIS is connected
to a network via the supplied ethernet cable. For further instructions refer to TIDAS S
300 UV/VIS instruction manual.
14. Shutter Button: Pushing the shutter butting the shutter is toggled. If the shutter is
open the LED is lit.
15. Cooling Fan. The fan dissipated the heat generated by the power supply and
electronics of the TIDAS S 300 UV/VIS. To ensure proper ventilation, the unit should
be situated away from walls or panels. Do not obstruct the cooling fan openings.
16. Input terminal for power cord and fuse. The TIDAS S 300 UV/VIS accepts voltages
from 80 to 240 VAC, 50-60Hz. Use correct cord and fuse, as described in the manual.

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17. Light output. SMA Fiber Optic Connector (collimator) with adjacent mounting
screws.
Fiber Optic Cables
18. Fiber optic cable —Light is coupled into the fiber optic input connector (1) of the
UltraPath 2 sample cell from the TIDAS S 300 UV/VIS light output (22) with a 600 μm
core fiber (WVLUXDUV-S-0600-SMA).
19. Fiber optic cable —Light is coupled from the output of the UltraPath 2 fiber optic
switch (4) into the TIDAS S 300 UV/VIS spectrometer Input (11) with a 600 μm core
fused silica fiber (WVLUXDUV-S-0600-SMA).
Peri-Star Pro Peristalitic Pump
20. Peri-Star Pro Pump Head. Two channels and eight rollers.
21. Control Panel. The Control Panel allows to start the pump clockwise and
counterclockwise, to increase and decrease pump speed between 0.1 rpm and 100
rpm, to stop the pump and to prime the pump (Refer to the Peri-Star Pro manual for
details).
22. Power Switch (ON/OFF). When the power switch is turned ON, power is supplied to
the Peri-Star Pro peristaltic pump

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23. Cooling Fan. The fan dissipated the heat generated by the power supply, motor and
electronics of the Peri-Star pump. To ensure proper ventilation, the unit should be
situated away from walls or panels. Do not obstruct the cooling fan openings.
24. Input terminal for power cord.
25. External Control socket. DB-15 connection to control speed and direction of the
pump head. Refer to the instruction manual for details.
Cleaning Kit (Available US Only)
26. Waveguide Cleaning Kit. The waveguide cleaning kit contains three solutions
specifically developed to keep the UltraPath 2 sample cell clean and ensure a high
measurement accuracy and repeatability.
LWCC Injection System
27. 6 Port Injection Valve
28. 6 Port Selection Valve
29. LWCC Injection System Connection Kit to connect between the switch and samples,
reference solutions and cleaning solutions on the LWCC injection system.
For a detailed description of the TIDAS S 300 UV/VIS and Peri-Star Pro, refer to the relevant
manuals.

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Setting up UltraPath 2
UltraPath 2 is a modular spectrophotometer system. Its main components are: UltraPath 2
sample cell and TIDAS S 300 UV/VIS spectrometer module. The assembly of the UltraPath 2
system is described below.
Parts List
Part Number
Quantity
Description
UPATH2-CELL
1
UltraPath 2 Cell
TIDAS S 300 UV/VIS
1
UV/VIS Spectrometer system (190-
720nm) including software, DH
Lightsource.
WVLUXDUV-S-0600-100-SMA
2
DUV Fiber (200-1200nm) 600um 100cm
SMA
WVLUXDUV-S-0200-100-SMA
1
DUV Fiber (200-1200nm) 200um 100cm
SMA
13395
2
SMA Bulkhead Feed Thru Connector, D-
hole
58006
1
Sample Injector Assembly for LWCC
58450
1
Kit, Adapter Syringe, LWCC
89372-2
1
LWCC Injection System
LOOP-5ML
1
Sample Loop Kit, 5 mL (2.5m PFA Tubing
1/16” ID x 1/8” OD + 2 x PEEK fittings 1/8"
OD Tubing x ¼”-28 UNF)
PERIPRO-4LS
1
Peristaltic Pump, 4 Channel, low speed
QFT2*
1
25mm GFF Filter and 10mm Cuvette
Holder, SMA
CUV2012-1*
1
Quartz Cuvette, Style C, 12.4x12.4x45mm,
10mm, 3.5ml
* Used when you need to measure particulate absorption on glass fiber filter pads (see
Setting up QFT2 Glass Fiber Filter section) –not used with the UltraPath 2 System setup.
Opening the package
Upon receipt of this system, make a thorough inspection of the contents and check for
possible damage. Concealed loss or damage should be reported at once to the carrier and an