WPI MICRO-ePUMP User manual

WORLD
PRECISION
INSTRUMENTS
MICRO-ePUMP
Microinjector
Serial No._____________________
www.wpiinc.com
INSTRUCTION MANUAL
012220

MICRO-ePUMP
World Precision Instruments i
CONTENTS
ABOUT THIS MANUAL ................................................................................................................... 1
INTRODUCTION .............................................................................................................................. 1
Features....................................................................................................................................... 2
Benets........................................................................................................................................ 2
Applications................................................................................................................................ 2
Notes and Warnings................................................................................................................. 3
Parts List...................................................................................................................................... 3
Unpacking................................................................................................................................... 3
INSTRUMENT DESCRIPTION ........................................................................................................ 4
Front Panel ................................................................................................................................. 4
Rear Panel................................................................................................................................... 5
Foot Switch ................................................................................................................................. 6
Touch Panel Display ................................................................................................................. 6
Setting Menu.............................................................................................................................. 7
Setup............................................................................................................................................ 8
OPERATING INSTRUCTIONS.......................................................................................................12
Saving a Protocol.....................................................................................................................15
Loading a Protocol..................................................................................................................15
Changing the Pipette..............................................................................................................16
TECHNIQUES IN MICROINJECTION...........................................................................................16
Setting the Compensation Pressure ..................................................................................16
Manufacturing Micropipettes ..............................................................................................18
Calibrating Volume by Measuring Droplet........................................................................18
Calibrating Rate using a Known Volume ...........................................................................19
Multibarrel Microinjection ....................................................................................................20
MAINTENANCE ..............................................................................................................................21
Cleaning.....................................................................................................................................21
ACCESSORIES.................................................................................................................................21
Replacement Parts .................................................................................................................21
TROUBLESHOOTING ...................................................................................................................22
SPECIFICATIONS............................................................................................................................23
APPENDIX A: DROPLET VOLUME..............................................................................................24
Volume of a Micropipette .....................................................................................................24
BIBLIOGRAPHY..............................................................................................................................25
INDEX...............................................................................................................................................26
WARRANTY .....................................................................................................................................27
Claims and Returns ................................................................................................................27
Repairs.......................................................................................................................................27
Copyright © 2020 by World Precision Instruments. All rights reserved. No part of this publication may
be reproduced or translated into any language, in any form, without prior written permission of World
Precision Instruments, Inc.

ii World Precision Instruments

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ABOUT THIS MANUAL
The following symbols are used in this guide:
This symbol indicates a CAUTION. Cautions warn against actions that can cause
damage to equipment. Please read these carefully.
This symbol indicates a WARNING. Warnings alert you to actions that can cause
personal injury or pose a physical threat. Please read these carefully.
NOTES and TIPS contain helpful information.
Fig. 1—MIRO-ePUMP Microinjector includes an internal pressurepump and the integrated
MICRO-ePORE Cell Penetrator technology.
INTRODUCTION
Designed to simplify intracellular injection and a variety of other microinjection tasks,
the MICRO-ePUMP uses carefully regulated air pres sure for injecting cells with uid.
Injected volumes range from picoliters to nanoliters. The port supplies positive pressure
for high-pressure ejection. The pressure port maintains a low positive “compensation”
pres sure to the injecting pipette between injection pulses to prevent uid up take
through capillary action.
Timing, injection pressure and compensation pressure are adjusted independent-
ly using the touch screen interface. Time intervals can range from 2 seconds down
to 10ms or less, depending on the injection pressure setting. The injection pressure
interval is triggered using the foot switch.

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The MICRO-ePUMP is designed to inject very small quantities of uids, such as drugs,
into cells or small organelles. Pressure injection is an especially useful alternative to
electroionophoresis, since it does not mandate the use of charged ions. Two dierent
positive pressures may be applied–one for ejection at high pressure and a second,
lower pressure to prevent back lling of the pipette by capillary action.
WPI’s MICRO-ePORE™ Pinpoint Cell Penetrator technology is embedded inside the
MICRO-ePUMP. When the researcher enables the The MICRO-ePORE™, it delivers
a highly localized voltage signal to a targeted injection site to facilitate penetration
with minimal trauma. The researcher determines the amplitude and frequency of the
signal that best suits the application. The signal originates in the control box, and it
is transmitted through the electrode interface cable to the microelectrode holder.
A silver wire is used to transmit the signal into the electrically conductive substance
being injected. A reference electrode is used to place the media at 0.0 V potential with
reference to the generated voltage.
The MICRO-ePUMP oers separate regulated compensation (back lling prevention)
and ejection pressures with a precision timing circuit that switches from injection
pressure to compensation pressure automatically.
Features
• Regulated compensation and injection pressure
• Pressure output: 0.3-87 PSI
• Onboard MICRO-ePORE™
• Pinpoint Cell Penetrator for targeted microinjection and increased viability of
injected embryos
• Audio continuity tone indicating active probe
• Injection counter to indicate total number of injections
• User-adjustable frequency and voltage through touch screen
• Foot switch to activate voltage for MICRO-ePORE™ and injection
• Low volume tubing assembly
Benets
• Internal pressure source
• Intuitive user-interface for injector and MICRO-ePORE™
• Small footprint takes up very little bench space
• Easy to navigate with touch screen and control knob
• Inject into a single cell in picoliter volumes
Applications
• Microinjection of diverse compounds and biomolecules – DNA, RNA, proteins
• Pre- and post-implantation in embryos of various species – mice, rats, monkeys,
bovine, pigs, zebrash, etc.

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Notes and Warnings
!WARNING: SECURE THE PIPETTE FIRMLY IN THE HOLDER. WHEN HIGH
PRESSURE IS APPLIED, A LOOSE PIPETTE CAN BE EJECTED FORCEFULLY.
DO NOT APPLY PRESSURES IN EXCESS OF 100 PSI (100 KPA).
!WARNING: THIS INSTRUMENT IS FOR INVESTIGATIONAL USE ONLY IN
ANIMALS OR OTHER TESTS THAT DO NOT INVOLVE HUMAN SUBJECTS.
Parts List
After unpacking, verify that there is no visible damage to the instrument. Verify that all
items are included:
(1) MICRO-ePUMP
(1) Foot switch
(2) 300863 WPI MICRO-ePORE™ Electrode Holders (Electrode holder includes an
electrode holder startup kit.)
(1) 300744 MICRO-ePUMP Capillary Kit which includes:
(4) 1.0 mm pipette gaskets (green)
(4) 1.2 mm pipette gaskets (black)
(4) 1.5 mm pipette gaskets (red)
(4) 1.65 mm pipette gaskets (white)
(1) Pipette handle
(1) MICRO-ePORE injection assembly
(2) Sealing o-rings
(1) Pipette holder
(1) MICRO-ePUMP tubing assembly
(1) MICRO-ePUMP adapter tubing assembly
(1) AC/DC 24 V Power Adapter
(1) Instruction Manual (available online at www.wpiinc.com/manuals)
Unpacking
Upon receipt of this instrument, make a thorough inspection of the contents and
check for possible damage. Missing cartons or obvious damage to cartons should be
noted on the delivery receipt before signing. Concealed damage should be reported
at once to the carrier and an inspection requested. Please read the section entitled
“Claims and Returns” on page 27 of this manual. Please contact WPI Customer
Service if any parts are missing at [phone] or [email protected].
Returns: Do not return any goods to WPI without obtaining prior approval (RMA
# required) and instructions from WPI’s Returns Department. Goods returned
(unauthorized) by collect freight may be refused. If a return shipment is necessary, use
the original container, if possible. If the original container is not available, use a suitable
substitute that is rigid and of adequate size. Wrap the instrument in paper or plastic
surrounded with at least 100 mm (four inches) of shock absorbing material. For further
details, please read the section entitled “Claims and Returns” on page 27 of this manual.

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INSTRUMENT DESCRIPTION
Front Panel
Touch screen interface
Adjustment Knob
Injector Port
MICRO-ePORE™ Interface Cable Port
Fig. 2—The major features of the MICRO-ePUMP are labeled in this diagram.
Touch Screen Interface – The responsive panel allows for intuitive control. Touch
the injection counter to reset it, the tank pressure gauge to rell the tank, Clear Tip to
blow a blast of air through the pipette tip, a parameter to modify it using the knob, or
the settings area to adjust the settings.
Adjustment Knob – Rotate the adjustment knob to change the highlighted
parameter. Press it to toggle between gross and ne tune controls.
MICRO-ePORE™ Interface Cable Port – If you are using the MICRO-ePORE™,
connect the Microelectrode Interface Cable (probe cable) to this port. Press the
connector into the port. This cable is labeled as Front Panel.
Fig. 3—The Interface Cable Connector is labeled “Front Panel.” Do NOT plug it into the foot
switch port on the rear panel.
Injector Port – Plug the easy connect connector on the tubing into this port.

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Rear Panel
Foot Switch Connection
USB Port (not used)
Power Supply Connection
Power Switch
Fig. 4—The foot switch connection, power supply connection and
power switch are located on the back panel of the MICRO-ePUMP.
Foot Switch Connection – Plug the connector from the foot
switch into the connection port marked with the foot switch icon.
Press the foot switch to initiate a timed sequence (timed mode)
or to manually eject pressure (manual mode). This foot switch is a
two-stage switch. Depress it half way down to engage the MICRO-
ePORE™, and depress it fully to make an injection. (See “Foot
Switch” on page 6.)
USB Port –This interface is reserved for factory use only.
Power Switch –To power on the unit, press the toggle switch to
—. To power o the unit, press the toggle switch to O.
Power Supply Connection –Insert the power supply connector
into this port and plug the other end into an A/C wall outlet.

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Foot Switch
The foot switch is a two-step switch. Press the switch half way down to activate the
MICRO-ePORE™. If the MICRO-ePORE™ is disabled, just press the switch all the way
down. When the switch is depressed all the way, the MICRO-ePUMP is activated.
Not depressed Half way depressed Fully depressed
Touch Panel Display
MICRO-ePORE™ Controller
Frequency
Amplitude
Injection Controller
Injection Pressure
(PSI or kPa)
Compensation Pressure
(PSI or kPa)
Injection Time (msec - 2 s max.)
Protocol Loaded
Injection Counter
Tank Pressure Gauge
Clear Clogged Tip
Settings Screen
2
Fig. 5—The Touch Panel Display gives you access to all the parameters and the settings menu.
Protocol Loaded – The number in the box in the upper left corner indicates the
protocol with stored parameters that is loaded. You can store and recall up to three
of your most used protocols in the MICRO-ePUMP memory. If the box is empty, no
protocol is loaded.
Injection Counter – Every time you depress the foot switch all the way (when the port
is enabled), the injection counter increments. To reset the injection counter to zero,
touch the injection counter on the display.
Tank Pressure Gauge – The internal pressure tank is pressurized to 100 PSI. When
the pressure in the tank falls below 90 PSI (90%), the tank automatically rells. This
10% is considered the tank reserve. The Tank Pressure Gauge shows the status
of the tank reserve as the pressure falls to 90%. If you want to manually start the
repressurization of the tank, touch the Tank Pressure Gauge. When you power up
the MICRO-ePUMP, the gauge may register below 90% as the tank lls initially. The
display will readout in PSI until the tank reaches 90 PSI. Then, the display will show the
percentage of the reserve pressure (0 – 100%).
Clearing Clogged Tip – Press Clear Tip to force a 500 ms burst of maximum pressure
(87 PSI_ through the injection tip. This can be used if the tip becomes clogged.

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Settings Screen – Touch anywhere in the settings area (bottom right corner) of the
screen to access the settings menu. This area also shows the status of the warning
tones and the pump mode (Timed or Manual).
Injection Controller
Injection Pressure – Touch the parameter and rotate the knob to adjust the pressure
used to inject. When you depress the foot switch all the way, this is the pressure that
is forced through the injection tip.
Compensation Pressure – Touch the parameter and rotate the knob to adjust the
compensation pressure that is used to prevent the back lling of the pipette.
Injection Time – Touch the parameter and rotate the knob to adjust the length of
each injection.
MICRO-ePORE™ Controller
Frequency – The WPI MICRO-ePORE™ Pinpoint Cell Penetrator delivers a highly
localized voltage signal to a targeted injection site to facilitate penetration with
minimal trauma. The researcher determines the amplitude and frequency of
the signal that best suits the application. If you are using the MICRO-ePORE™
techcnology, touch the parameter and rotate the knob to adjust the frequency of
the signal delivered. To engage the MICRO-ePORE™, depress the foot switch half
way down. When you press the foot switch all the way down, the MICRO-ePORE™
disengages and the injection is made. To disable the MICRO-ePORE™, see “Setting
Menu” on page 7.
Amplitude – If you are using the MICRO-ePORE™ techcnology, touch the parameter
and rotate the knob to adjust the amplitude of the signal delivered when you depress
the foot switch half way down.
Setting Menu
Injection Settings
Mode
Units
MICRO-ePORE™ Settings
Status
Continuity Test Tone
PUMP Settings
Save/Load Protocols
Port Status (On/Off)
Back Button
Fig. 6—From the Settings menu, you can save and load parameters, choose the mode, set
the units, toggle the MICRO-ePORE™ o or on, silence the tones, and toggle the injection
port o or on.
Mode – Choose either the Manual or the Timed checkbox to determine the injection

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mode. Manual control allows for injection pressure to be applied for as long as the
foot switch is fully depressed. Timed injections use the duration set on the main screen.
Units – Select one of the checkboxes to set the display to either PSI or kPa.
Status – Select the ON checkbox to enable the MICRO-ePORE™ or select the OFF
checkbox to disable the MICRO-ePORE™. When the MICRO-ePORE™ is disabled, the
bottom section of the main display is blank.
Continuity Test Tone – By default, tones are disabled when you start the MICRO-
ePUMP. If tones are enabled, a tone sounds when the circuit between the probe tip
and the reference electrode is not complete. Without a complete circuit, the MICRO-
ePORE™ will not be able to apply current. Every time you depress the foot switch half
way (when the MICRO-ePORE™ is enabled, a continuity test is conducted. A good
tone sounds if the circuit is complete. Otherwise a warning tone sounds. To silence
the tone while you correct a setup error, select the Silent check box. Select the Tone
checkbox to enable the tones.
Save/Load Protocols – The MICRO-ePUMP can store your three favorite parameter
sets (protocols). On the main screen set the parameters as desired. Then press one of
the Save buttons. For example, press SAVE1 to save the parameters as Protocol 1. To load
a set of saved parameters (a protocol), press the appropriate LOAD button. The number
of the loaded protocol displays in the box in the upper left corner of the main screen.
Port Status – Port ON indicates that the injection port is enabled. To disable the port,
touch the Port ON button. The button will change to Port OFF. To re-enable the port,
press the Port OFF button, and the button will change to Port ON.
Back Button – Touch the Back button to return to the main screen.
Setup
1. With the MICRO-ePUMP turned o, make the following connections:
• Connect Power Supply to the rear of the unit and plug it into a wall outlet.
• Plug the Foot Switch Connector into the Foot Switch Port.
Fig. 7—The Foot Switch Connector is labeled “Rear Panel.” Do NOT plug it into the injection
port on the front panel.
NOTE: DO NOT connect the capillary kit hose to the MICRO-ePUMP.
2. Verify that the capillary kit hose is disconnected from the Injection Port. Then,
power on the MICRO-ePUMP. On power up, the following happens:
• The power up screen appears.
• The MICRO-ePUMP performs a self-test.
• The operating pressure begins to build.
After the self-test completes, the main display screen appears. See “Touch
Panel Display” on page 6. It displays the conguration settings used when it
powered o the last time.

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Fig. 8—(Left) The power up screen shows the software revision on your MICRO-ePUMP.
Fig. 9—As the self-test runs through the programmed diagnostic sequence, the display
shows the results.
3. Press the touchscreen to access the main screen. By default, the injection port is
disabled. Tap the warning or the Settings icon to enable the Injection Port.
Fig. 10—The warning indicates that the injection port is disabled by default on startup.
Assembling the Capillary Kit
Pipette Holder
Pipette Handle
Tubing Assembly
Adapter Tubing Assembly
Gasket
Cap
Pipette
Tubing connected to the barb
Body
Fig. 11—The 300744 Capillary Kit must be properly assembled.
1. Slide the bare end of the Adapter Tubing Assembly through the Pipette Handle.
2. Connect it to the barb of the Pipette Holder Body. Be careful so that the barb
does not break when attaching or removing the tubing.
3. Slide the Pipette Handle over the barb and carefully screw it onto the Body of the
Pipette Holder. Be careful not to cross thread it.
4. Place a gasket of the correct size in the cap. Refer to the table below. Then, insert
the blunt end of the micropipette (pulled capillary glass) into the cap through
the gasket and into the body. Screw the cap in place. The screw cap and rubber
gasket rmly hold the glass micropipette.
Gasket Color Green Black Red White
Pipette Diameter (mm) 1.0 1.2 1.5 1.65

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!
WARNING: DANGER OF INJURY EXISTS IF THE PIPETTE IS INSECURE. HIGH
PRESSURE CAN CAUSE EJECTION AT HIGH VELOCITY.
5. The other end of the small tubing has a quick connector. Connect it with the blue
MICRO-ePUMP Tubing Assembly provided.
6.
Verify that the Injection Port is disabled. If not, touch the Settings area of the display
to access the Settings menu and touch the Port ON button to set it to Port OFF .
7. Align the easy-connect Injector connector with the injector port on the front of
the MICRO-ePUMP, slide the connector securely into the port and attach it with a
slight clockwise twist.
Fig. 12—(Left) Align the connector with the Injection Port, slide the connector in position and
rotate the connector a little clockwise to lock it into place.
Fig. 13—(Right) The PicoNozzle is connected to the injection port.
Setting up the MICRO-ePORE™
WARNING: GLASS PIPETTE SHOULD BE SECURED TIGHTLY BEFORE
MAKING INJECTIONS.
1. The pipette holder has a silver wire that must be inserted into the glass
micropipette. The wire must be long enough to make contact with the substance
to be injected. This wire is intentionally left longer than most pipettes to allow for
dierent lengths. Trim the wire to the preferred length.
Fig. 14—This pipette holder is designed for use with the WPI MICRO-ePUMP.
2. The Microelectrode Interface Cable has two 2 mm male pin connectors.

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Fig. 15—The Microelectrode Interface Cable has two connectors, one for the reference
electrode and one for the microelectrode holder.
• The red pin connects to the 2 mm socket on the microelectrode holder.
Insert the red pin into the socket on the microelectrode holder.
Fig. 16—The red pin of the microelectrode interface cable connects to the microelectrode
holder.
• The black pin of the microelectrode interface cable connects to the 2 mm
socket on one of the reference wires. This is necessary to complete the
electrical connection to solution. For the well-style electrode, insert the black
pin into the socket of the reference electrode (Fig. 17). For the probe-style
electrode, the black pin of the microelectrode interface cable connects to
the back side of the reference electrode block. The probe-style electrode pin
plugs into the front of the block (Fig. 18).
Fig. 17—(Left) The well-style electrode is properly connected. [Ring reference electrode – OD:
12.7 mm (0.5”), Thickness: 0.4 mm (0.16”)]
Fig. 18—(Right) The probe-style electrode is properly connected.
3. A. Well-style electrode–If you use the well-style electrode, the gold ring must be
secured to your slide using vacuum grease to create an impermeable temporary
seal. Place the liquid medium with your embryos inside the gold ring.
B. Probe-style electrode–If you are using the probe-style electrode, place the
block near the injection site. Then, bend the bare reference wire as needed to
make contact with the liquid medium in order to complete the circuit.

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OPERATING INSTRUCTIONS
Changing the Injection Parameters
1. Press anywhere in the settings area to access the Settings menu. Press Port O to
turn on the pressure port (PORT ON).
Fig. 19—(Left) Touch the setting area in the bottom right corner to access the Settings menu.
Fig. 20—(Right) Touch the Port OFF button to enable the injection port.
2. To change the parameters, just touch the screen on the parameter you want to
change. A box appears around the parameter to highlight it. Adjust the parameter
by turning the knob. To change the dial adjustment speed, press the knob on the
front of the MICRO-ePUMP. The snail changes the display in 0.01 increments,
and the running man changes it in increments of 0.1.
Fig. 21—(Left) Touch the parameter you want to adjust and rotate the knob to modify the
parameter.
Fig. 22—(Right) Press the knob to toggle between gross and ne tuning adjustments.
3.
Two seconds after a parameter is adjusted, it is saved into the memory. If you move
from one parameter to the next before two seconds, the rst parameter is automatically
saved into the memory as the working parameter. This setting will be recalled on power
up.
NOTE: This is dierent from saving parameter sets (protocols) to load.

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Changing the MICRO-ePORE™ Parameters
1. When the MICRO-ePORE™ is disabled, the home screen does not show the
MICRO-ePORE™ parameters in the bottom half of the screen. To enable the
MICRO-ePORE™, touch the Settings area of the main screen to go to Settings
menu.
Fig. 23—The MICRO-ePORE™ is disabled in this conguration. No parameters show in the
bottom half of the screen.
2. Tap a parameter and turn the knob to change the parameter.
Fig. 24—Touch the Frequency parameter or the Amplitude parameter and rotate the knob.
3.
Two seconds after a parameter is adjusted, it is saved into the memory. If you move
from one parameter to the next before two seconds, the rst parameter is automatically
saved into the memory as the working parameter. This setting will be recalled on power
up.
NOTE: This is dierent from saving parameter sets to load.

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Relling the Air Tank
1. The internal pump reservoir automatically replenishes itself when it fall below
90% of its full capacity.
2. To reset the pump manually, press the gauge meter on the touchscreen, and the
reservoirs starts relling.
Fig. 25—(Left) Touch the gauge to start relling the reservoir.
Fig. 26—(Right) When the tank is fully charged at 100 PSI, the tank reserve gauge is green.
Resetting the Injection Counter
This area in the upper left corner shows the injection count. Whenever the foot switch
is fully depressed, the injection counter increments. Reset it to zero by pressing on the
Injections area of the main display touchscreen.
Adjust the MICRO-ePUMP Settings
The setting section (bottom right corner of the main screen) displays the current settings:
• MICRO-ePORE™ status (O or On)
• MICRO-ePUMP mode (Manual or Timed)
Fig. 27—(Left) The Setting area is located in the bottom right corner of the main screen.
Fig. 28—(Right) Touch the Setting area to display the Settings menu.
1. To modify these settings, press the Settings section on the touchscreen.
2. From there, you can make the following adjustments:
• Disable or enable the MICRO-ePORE™.

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• Disable or enable the MICRO-ePORE™ continuity test tone.
• Change the MICRO-ePUMP mode (manual or timed).
• Change the pressure units displayed on the main screen (PSI or kPa).
3. Press the BACK button to save the settings and return to the main screen.
Saving a Protocol
After you have adjusted the parameters, you can save the set of parameters as a
protocol. MICRO-ePUMP will store up to three (3) protocols.
1. Set the parameters, as desired.
2. Press the Settings section on the touchscreen to access the Settings menu.
Fig. 29—The Settings menu lets you save a protocol or load a saved protocol.
3. To save the existing set of parameters from the main screen, press the SAVE1,
SAVE2 or SAVE3 button. The parameters are saved in Protocol 1, Protocol 2 or
Protocol 3, depending on which SAVE button you chose.
4. Press the BACK button to return to the main screen.
Loading a Protocol
Saved protocols may be recalled, and this loads the saved parameters for use. When
a protocol is loaded, the protocol number (1, 2 or 3) appears in the box located in the
upper left corner of the main screen.
1. Press the Settings section on the touchscreen to access the Settings menu.
Fig. 30—The Settings menu lets you save a protocol or load a saved protocol.
2. To bring up a saved protocol with the MICRO-ePUMP parameters, press the
LOAD 1, LOAD2 or LOAD3 button. The unit loads the saved parameters of
Protocol 1, Protocol 2 or Protocol 3, depending on which LOAD button you chose.
3. Press the BACK button to return to the main screen.

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Changing the Pipette
To change the pipette, turn o the injection PORT to avoid depleting the reservoir. The
button indicates the present state of the port.
1. To turn o the Injection Port, press the Settings section on the touchscreen.
Fig. 31—The Settings menu lets you save enable or disable the Injection Port by pressing the
Port ON/Port OFF toggle button.
2. Press the Port ON button to disable the Injection Port
3. Press the BACK button to save the settings and return to the main screen. A
warning indicates that the Injection Port is disabled.
Fig. 32—When the Injection Port is disabled a notice appears on the main screen.
4. To install the micropipette, see “Assembling the Capillary Kit” on page 9.
5. When the micropipette is properly installed, click on the Settings area to access
the Settings menu and press the Port OFF button to enable the Injection Port.
TECHNIQUES IN MICROINJECTION
The MICRO-ePUMP was designed for demanding tasks like the microinjection of uid
into cells. In this section, we will look at several important things to keep in mind when
working with the MICRO-ePUMP.
Setting the Compensation Pressure
The compensation pressure is used to counterbalance the capillary action of the uid
backlling into the pipettes. If you insert an empty pipette into uid, you can see a
meniscus rising from the capillary tip.
Set the Compensation Pressure parameter by touching the Compensation Pressure
parameter on the main screen and adjusting the knob until the meniscus stops at the
desired position in the micropipette. In many applications, colored dye or uorescent