Zeiss Axioskop 2 plus User manual

Operating Manual Axioskop 2
plus
/Axioskop 2
mot
plus
Routine Microscope

INTRODUCTION Axioskop 2 plus
Carl Zeiss Copyright Axioskop 2 mot plus
0-2 B 40-075 e 10/03
Knowledge of this manual is required for the operation of the instrument. Would you therefore please
make yourself familiar with the contents of this manual and pay special attention to hints concerning the
safe operation of the instrument.
The specifications are subject to change; the manual is not covered by an update service.
© Unless expressly authorized, forwarding and duplication of this document and the software
package, and the utilization and communication of its contents are not permitted. Violations will
entail an obligation to pay compensation.
All rights reserved in the event of granting of patents or registration of a utility model.
Issued by: Carl Zeiss
Light Microscopy
P.O.B. 4041
37030 Göttingen
GERMANY
Phone: ++49 551 5060 660
Telefax: ++49 551 5060 464
Internet: www.zeiss.de/micro
E-Mail: [email protected]
Number of this manual: B 40-075 e
Date of issue: 31st of October 2003

Axioskop 2 plus INTRODUCTION
Axioskop 2 mot plus Contents Carl Zeiss
B 40-075 e 10/03 0-3
CONTENTS:
Page
INTRODUCTION...........................................................................................................0-2
Copyright......................................................................................................................0-2
Contents .......................................................................................................................0-3
Microscopy in a few steps..............................................................................................0-7
Notes on instrument safety............................................................................................0-8
Notes on warranty.......................................................................................................0-12
Overall view of Axioskop 2 plus ...................................................................................0-13
Overall view of Axioskop 2 mot plus ............................................................................0-14
CHAPTER 1 INSTRUMENT DESCRIPTION .......................................................................................1-2
1.1 Name and intended application.....................................................................................1-2
1.2 Instrument description and main features ......................................................................1-2
1.3 Axioskop 2 plus (manual) microscope configurations and modules ................................1-4
1.4 Axioskop 2 mot plus microscope configurations and modules........................................1-8
1.5 Objectives....................................................................................................................1-12
1.6 Eyepieces.....................................................................................................................1-14
1.7 Stage micrometers and eyepiece reticles ......................................................................1-14
1.8 Technical data .............................................................................................................1-16

INTRODUCTION Axioskop 2 plus
Carl Zeiss Contents Axioskop 2 mot plus
0-4 B 40-075 e 10/03
Page
CHAPTER 2 START-UP ....................................................................................................................2-4
2.1 Initial start-up................................................................................................................2-4
2.1.1 Unpacking and installation of the microscope................................................................2-4
2.1.2 Attachment of binocular tube or phototube ..................................................................2-5
2.1.3 Insertion of eyepieces and centering telescope ..............................................................2-5
2.1.4 Setting of the interpupillary distance of the binocular tube ............................................2-7
2.1.5 Setting of viewing height ..............................................................................................2-7
2.1.6 Screw in of objectives....................................................................................................2-7
2.1.7 Attachment of universal condenser................................................................................2-8
2.1.8 Connection to the line...................................................................................................2-9
2.1.9 Switch microscope and HBO 100 W transformer on and off ..........................................2-9
2.1.10 HAL 100 halogen illuminator.......................................................................................2-10
2.1.11 HBO 103 illuminator....................................................................................................2-11
2.1.12 Connect PC / Laptop to the Axioskop 2 mot plus microscope ......................................2-15
2.1.13 Attachment of round cable holder...............................................................................2-15
2.2 Attachments and conversions......................................................................................2-16
2.2.1 Changing the condenser .............................................................................................2-16
2.2.2 Changing the phase stop for phase contrast or darkfield in the universal condenser ....2-17
2.2.3 Changing the DIC prism in the universal condenser .....................................................2-18
2.2.4 Attachment of filter mount..........................................................................................2-18
2.2.5 Attachment of D polarizer ...........................................................................................2-19
2.2.6 Attachment of SENARMONT DIC polarizer...................................................................2-19
2.2.7 Changing the HAL 100 halogen lamp..........................................................................2-20
2.2.8 Changing the HBO 103 W/2 mercury pressure short-arc lamp .....................................2-20
2.2.9 Changing the mechanical stage...................................................................................2-23
2.2.10 Changing the binocular tube.......................................................................................2-26
2.2.11 Attachment of intermediate tube with height adjustment............................................2-27
2.2.12 Attachment of adapter for the Axioskop 2 plus ...........................................................2-28
2.2.13 Installing and Removing “Push&Click” Modules ..........................................................2-29
2.2.14 Changing the filter set in the FL P&C reflector module.................................................2-30
2.2.15 Changing the beam splitter in the FL P&C reflector module .........................................2-30
2.2.16 Attachment of active coobservation equipment for 2 users..........................................2-32
2.2.17 Attachment of multi-observation equipment ...............................................................2-34
2.2.18 Attachment of intermediate tube for image projection ................................................2-36

Axioskop 2 plus INTRODUCTION
Axioskop 2 mot plus Contents Carl Zeiss
B 40-075 e 10/03 0-5
Page
CHAPTER 3 OPERATION .................................................................................................................3-3
3.1 Overview of operation and function controls .................................................................3-3
3.1.1 Operation and function controls of the Axioskop 2 plus.................................................3-4
3.1.2 Overview of operation and function controls of the Axioskop 2 mot plus.....................3-10
3.2 Switching on and basic settings ...................................................................................3-18
3.2.1 Axioskop 2 plus ...........................................................................................................3-18
3.2.2 Axioskop 2 mot plus....................................................................................................3-18
3.3 Illumination and contrasting techniques.......................................................................3-24
3.3.1 Setting of transmitted-light brightfield according to KÖHLER .......................................3-24
3.3.2 Setting of transmitted-light darkfield ...........................................................................3-27
3.3.3 Setting of transmitted-light phase contrast ..................................................................3-29
3.3.4 Setting of transmitted-light polarization.......................................................................3-31
3.3.5 Setting of transmitted-light differential interference contrast (DIC)..............................3-36
3.3.6 Setting of epi-fluorescence ..........................................................................................3-39
3.4 Documentation ...........................................................................................................3-41
3.4.1 Attachment of photomicrography equipment..............................................................3-41
3.4.2 Attachment of videomicroscopy equipment.................................................................3-43
3.4.3 Attachment of adapter for digital compact cameras ....................................................3-44
3.5 Multi-observation equipment.......................................................................................3-45
3.5.1 Attachment of active coobservation equipment for 2 users.........................................3-45
3.5.2 Attachment of multi-observation equipment ...............................................................3-47
3.6 Drawing device............................................................................................................3-49
3.6.1 Intermediate tube for image projection, switchable .....................................................3-49
3.7 Quantitative microscopy ..............................................................................................3-50
3.7.1 Measurement of lengths..............................................................................................3-50
3.7.2 Height measurement ...................................................................................................3-51

INTRODUCTION Axioskop 2 plus
Carl Zeiss Contents Axioskop 2 mot plus
0-6 B 40-075 e 10/03
Page
CHAPTER 4 CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE .....................................4-2
4.1 Instrument care .............................................................................................................4-2
4.2 Instrument maintenance................................................................................................4-3
4.2.1 Performing checks.........................................................................................................4-3
4.2.2 Changing the fuses .......................................................................................................4-3
4.3 Troubleshooting ............................................................................................................4-4
4.4 Spares, consumables and tools ......................................................................................4-8
4.5 Requesting service.........................................................................................................4-9
ANNEX........................................................................................................................ A-1
List of abbreviations ..................................................................................................... A-2
List of key words .......................................................................................................... A-4
Patent rights................................................................................................................. A-8

Axioskop 2 plus INTRODUCTION
Axioskop 2 mot plus Microscopy in a few steps... Carl Zeiss
B 40-075 e 10/03 0-7
Microscopy in a few steps using the example of "Setting of transmitted-light brightfield
according to KÖHLER"
☞Please read the instructions regarding instrument safety and the Description (Chapter 1) and
Start Up (Chapter 2) chapters before using the Axioskop 2 plus and/or Axioskop 2 mot plus
microscopes for the first time.
(1) As described in chapter 2, the microscope is ready for operation and switched on according to
section 3.3.
(2) Place a high-contrast specimen on the mechanical stage (cover slip pointing upwards).
(3) Swing in 10x objective on the nosepiece and focus on the specimen; if possible, always move
the specimen away from the objective!
(4) Move condenser - e.g. universal condenser 0.9 - (in brightfield position, front lens switched in)
to the upper stop position via the condenser drive. The height adjustment must be set in such
a way that the specimen is not touched by the condenser.
(5) Close luminous-field diaphragm until it is visible in the field of view, even if not in focus
(Fig. 0-1/A).
(6) Lower universal condenser 0.9 until the edge of the luminous-field diaphragm appears in focus
(Fig. 0-1/B).
(7) Center luminous-field diaphragm using centering screws on the condenser carrier (Fig. 0-1/C)
and open it until its edge just disappears behind the field of view (Fig. 0-1/D).
(8) To set the aperture diaphragm (contrast), remove one eyepiece from the binocular tube and
set the aperture diaphragm to approx. 2/3 of the diameter of the objective exit pupil
(Fig. 0-1/E). Optimum contrast setting is dependent on the respective specimen.
(9) Insert eyepiece again and, if required, refocus on the specimen via fine drive.
(10) After each objective change, field size and objective aperture change, which makes it
necessary to repeat steps (5) to (9).
(11) For objective magnifications between 1.25x and 5x, the front lens 0.9 of the universal
condenser must be swung out and the aperture diaphragm opened entirely.
Fig. 0-1 Diaphragm settings in transmitted-
light brightfield according to
KÖHLER

INTRODUCTION Axioskop 2 plus
Carl Zeiss Notes on instrument safety Axioskop 2 mot plus
0-8 B 40-075 e 10/03
Notes on instrument safety
The Axioskop 2 plus and Axioskop 2 mot plus microscopes have been designed produced and tested in
compliance with DIN 61010-1 (IEC 1010-1) and DIN EN 61010-2-101 safety requirements for electrical
measuring, control and laboratory instruments.
They meet the requirements of the directive 98/79/EC and are marked with the marking.
This operation manual includes information and warnings which must be observed by the user.
The following warning and information symbols are used in this manual:
☞NOTE
This symbol is a warning which you must observe under all circumstances.
CAUTION
This symbol is a warning which indicates a hazard to the instrument or instrument system.
CAUTION
This symbol is a warning which indicates a hazard to the user of the instrument.
CAUTION
Hot surface!
CAUTION
UV radiation is emitted!
CAUTION
Disconnect the instrument from the line before opening it!

Axioskop 2 plus INTRODUCTION
Axioskop 2 mot plus Notes on instrument safety Carl Zeiss
B 40-075 e 10/03 0-9
The Axioskop 2 plus and Axioskop 2 mot plus microscopes, including original accessories, may only be
used for the microscope techniques described in this manual.
Particular attention must be paid to the following warning notes:
The manufacturer cannot assume any liability for any other applications, possibly also involving
individual modules or single parts. This also applies to all service or repair work which is not
carried out by authorized service personnel. Furthermore, this forfeits all the claims against
warranty.
The power plug must be inserted in a socket featuring a grounding (earth) contact. The
grounding effect must not be made ineffective by an extension cable which does not have a
protective ground wire.
If it is established that protection measures are no longer effective, the instrument must be
switched off and safeguarded against inadvertent operation. Please contact a Zeiss service
agency or Carl Zeiss Microscopy Service for repairing the instrument.
☞
The power unit which is integrated in the stand of the microscopes permits the use of line
voltages in the range between 100 and 240 V ± 10 %, 50 / 60 Hz, without having to change
the voltage on the instrument.
The ballasts for the HBO 103 (ebg 100 dc) or XBO 75 (ebx 75 isolated) are designed for the 90
to 250 V AC, 50 to 60 Hz range. The instruments adapt automatically to the available line
voltage. There is no need to change the voltage.
However, the transformer for the HBO 50 must be set manually to the correct voltage (100,
110, 120, 127 or 230, 240 V), and the correct frequency must be preselected (50 or 60 Hz).
Before switching on the instrument, check whether it is suitable for the line voltage present.
Before switching on the instrument, check whether it is suitable for the line voltage present.
Always disconnect the instrument from the line before opening the instrument and before
changing the fuses.
Make sure to use only fuses of the rated power required. The use of makeshift fuses and the
short-circuiting of the fuse holders are not permitted.

INTRODUCTION Axioskop 2 plus
Carl Zeiss Notes on instrument safety Axioskop 2 mot plus
0-10 B 40-075 e 10/03
When the stand with motorized focusing drive is used, there is a risk of crushing between stage
carrier / condenser carrier and stand base. Therefore, avoid making any adjustments under the
stage carrier when the stage is lowered. For safety reasons, the stage lowering procedure can
be automatically switched off (see page 3-13).
The Axioskop 2 plus and Axioskop 2 mot plus microscopes are not equipped with any special
devices for protection from substances which are corrosive, potentially infectious, toxic,
radioactive or other substances which could be hazardous to health. All the legal regulations,
particularly the pertinent accident prevention regulations of the respective country, must be
observed when handling such substances.
Gas discharge lamps, e.g. HBO 103, emit ultraviolet radiation which can cause burns on the
eyes and skin. Therefore, never look directly into the light of these lamps and avoid direct,
unprotected incidence of their light on your skin. When using the microscope, always use the
protective devices belonging to the instrument (e.g. special attenuation filters). When hot, gas
discharge lamps are under high internal pressure and may therefore only be changed when
they have cooled down, and protective gloves and goggles must be used (for detailed
information please see Operating Manual B 40-065 e).
When fluorescence filters are used, the filter protecting from heat emitted by the microscope
illuminator must not be removed, since fluorescence filters are sensitive to heat and their
performance might therefore be impaired.
Avoid touching the hot lamp housing. Always pull the power plug before changing the lamps
and allow the instrument to cool down for approx. 15 mins.
Dust and dirt can impair the performance of the instrument. Therefore, the instrument must be
protected against these influences as far as possible, and covered with the dust cover if it is not
used for longer periods of time. Always check whether the instrument is switched off before
you cover it.
Placing objects against or covering ventilation slats can lead to a build-up of heat which will
damage the instrument and, in extreme cases, cause a fire. Always keep the ventilation slats
clear and make sure that no objects enter the instrument through the ventilation slats.
Defective microscopes should not be placed in ordinary domestic waste; they should be
disposed of in accordance with the relevant statutory provisions.

Axioskop 2 plus INTRODUCTION
Axioskop 2 mot plus Notes on instrument safety Carl Zeiss
B 40-075 e 10/03 0-11
The instruments may only be operated by trained personnel who must be aware of the possible
danger involved with microscopy and the relevant application. The Axioskop 2 plus / Axioskop 2
mot plus is a high-precision instrument which can be impaired in its performance or destroyed
when handled improperly.
☞Be sure to read the safety notes provided with Immersol 518 N®immersion oil.
Immersol 518 N®immersion oil irritates the skin. Avoid any contact with skin, eyes and
clothing.
After skin contact, wash the oil off with plenty of water and soap.
After eye contact, immediately rinse the eye with plenty of water for at least five minutes.
If the irritation persists, consult a specialist.
Proper disposal (Immersol 518 N):
Take care to ensure that immersion oil does not enter surface water or the sewage system.

INTRODUCTION Axioskop 2 plus
Carl Zeiss Notes on warranty Axioskop 2 mot plus
0-12 B 40-075 e 10/03
Notes on warranty
The manufacturer guarantees that the instrument has no material and production defects when
delivered. You must inform us of any defects immediately and we must do anything to minimize the
damage. If the manufacturer is informed of such a defect, he is obliged to remove it; it is his decision
whether he does this by repairing the instrument or by delivering an instrument free of any defect. No
guarantee is provided for defects caused by natural wear (wearing parts in particular) and improper use.
The instrument manufacturer is not liable for damage caused by faulty operation, negligence or any other
meddling with the instrument, particularly the removal or replacement of instrument components, or the
use of accessories from other manufacturers. This forfeits all the claims against warranty.
With the exception of the work specified in this manual, no maintenance or repair of the
Axioskop 2 plus / Axioskop 2 mot plus may be performed. Repairs may only be performed by Zeiss service
staff or specially authorized personnel. Should any defect occur with the instrument, please get in touch
with your local Zeiss agency.

Axioskop 2 plus INTRODUCTION
Axioskop 2 mot plus Overall view of the Axioskop 2 plus Carl Zeiss
B 40-075 e 10/03 0-13
Overall view of the Axioskop 2 plus
1 Eyepieces
2 Binocular tube
3 Stand
4 Incident-light illuminator
5 Transmitted-light illuminator
6 Manual universal condenser
7 Mechanical stage
8 Objectives
10
20
30
40
50
60
70
80
90
100

INTRODUCTION Axioskop 2 plus
Carl Zeiss Overall view of the Axioskop 2 mot plus Axioskop 2 mot plus
0-14 B 40-075 e 10/03
Overall view of the Axioskop 2 mot plus
1 Eyepieces
2 Binocular ergotube
3 Stand
4 Incident-light illuminator
5 FL excitation filter wheel
6 Transmitted-light illuminator
7 Motorised universal condenser
8 Mechanical stage
9 Objectives

Axioskop 2 plus INSTRUMENT DESCRIPTION
Axioskop 2 mot plus Contents / List of illustrations Carl Zeiss
B 40-075 e 10/03 1-1
INSTRUMENT DESCRIPTION
Contents
1Instrument Description ...................................................................................................1-2
1.1 Name and intended application..........................................................................................1-2
1.2 Instrument description and main features...........................................................................1-2
1.3 Axioskop 2 plus (manual) microscope configurations and modules .....................................1-4
1.4 Axioskop 2 mot plus microscope configurations and modules ............................................1-8
1.5 Objectives ........................................................................................................................1-12
1.6 Eyepieces .........................................................................................................................1-14
1.7 Stage micrometers and eyepiece reticles...........................................................................1-14
1.8 Technical Data .................................................................................................................1-16
List of illustrations
Fig. 1-1 Axioskop 2 plus (manual) microscope configurations..........................................................1-4
Fig. 1-2 Axioskop 2 mot plus microscope configurations .................................................................1-8
Fig. 1-3 Objective..........................................................................................................................1-12

INSTRUMENT DESCRIPTION Axioskop 2 plus
Carl Zeiss Name ... / Instrument description ... Axioskop 2 mot plus
1-2 B 40-075 e 10/03
1 INSTRUMENT DESCRIPTION
1.1 Name and intended application
Manufacturer's name: Axioskop 2 plus microscope
Axioskop 2 mot plus microscope
The Axioskop 2 plus and Axioskop 2 mot plus microscopes have been designed as universally applicable
microscopes for applications in biology and medicine, e.g. for the examination of blood and / or tissue
samples from the human body. They can be used as pure transmitted-light microscopes or, with epi-
fluorescence equipment, as combined transmitted-light / incident-light microscopes.
The Axioskop 2 plus and Axioskop 2 mot plus microscopes are mainly used in:
− hospitals
− doctor's offices
− universities and
− industry
1.2 Instrument description and main features
With the advanced pyramid and modular design, the Axioskop 2 plus incorporates time-tested principles
in microscope construction, thus ideally combining modern demands on design, operating convenience
and function with technical performance. Thanks to the modular design, existing microscope modules of
the Axioskop 2 plus, such as tubes, intermediate tubes and condensers, are easy to attach.
The Axioskop 2 plus is offered as a manual and a motorized / coded version. The basic stand is equipped
with either manual (Axioskop 2 plus) or motorized / coded operation and function controls
(Axioskop 2 mot plus), but is otherwise identical for both versions.
Depending on the instrument configuration, the following microscopy and contrasting techniques are
possible:
Transmitted light: Incident light:
- brightfield - fluorescence contrast
- darkfield
- phase contrast
- polarization contrast
- differential interference contrast (DIC)
If ordered, the optional epi-fluorescence equipment including the adjusting aid is integrated in the
microscope stand on delivery. For technical reasons, retrofitting of existing instruments with the epi-
fluorescence equipment should be performed by Zeiss service staff.
The binocular tubes and the suitable adapters allow one microscope camera, one SLR camera or one
video camera to be attached for documentation purposes.

Axioskop 2 plus INSTRUMENT DESCRIPTION
Axioskop 2 mot plus Instrument description and main features Carl Zeiss
B 40-075 e 10/03 1-3
Major instrument features of the Axioskop 2 plus (manual):
− Stable and sturdy diecast stand with T-shaped base in the pyramid design and integrated DC supply
for the HAL 12 V 100 W halogen lamp
− Stage focusing via Harmonic Drive TM gear and coaxial drive
− Fixed and rotary mechanical stage with ceramic-coated surface and extended drive for higher
operating convenience
− High-performance HAL 12 V 100 W illuminators for transmitted light and HBO 103 for incident light
− Achromatic-aplanatic universal condenser 0.9 with swing-in front lens for applications in brightfield,
darkfield, phase contrast, polarization contrast and differential interference contrast
− Ball-bearing, 5-position nosepiece with W 0.8" thread
− ICS objectives in the price performance categories A-Plan, Achroplan, Plan-Neofluar, Plan-Apochromat
− Binocular tubes and phototubes with fixed and variable viewing angle
− 10x focusing eyepieces with field number 23, suitable for spectacle wearers
− High-performance epi-fluorescence equipment
− Wide variety of attachment possibilities for photography and videomicroscopy
− Multi-observation equipment for 2, 9 or more viewing stations
− Drawing device with intermediate tube for image projection
In addition to the above instrument features, the Axioskop 2 mot plus has the following features which
differ from the manual version:
− High-precision step motor drive for stage focusing
− Motorized condenser functions (front lens, turret disk, aperture diaphragm), optional
− Coded objective nosepiece
− Coded reflector turret
− Objective-dependent brightness control in combination with the coded nosepiece
− Automatic matching of the focusing speeds and focus positions of the objectives used
− Optional and motorized, 8-position FL excitation filter wheel (filter diameter 25 mm) for incident-light
fluorescence, controlled by step motor
− The microscope can be connected to a PC via the RS 232 interface

INSTRUMENT DESCRIPTION Axioskop 2 plus
Carl Zeiss Axioskop 2 plus microscope configurations ... Axioskop 2 mot plus
1-4 B 40-075 e 10/03
1.3 Axioskop 2 plus (manual) microscope configurations and modules
Fig. 1-1 Axioskop 2 plus (manual) microscope configurations
1
1
1
1
1
10
20
30
40
50
60
70
80
90
100

Axioskop 2 plus INSTRUMENT DESCRIPTION
Axioskop 2 mot plus Axioskop 2 plus microscope configurations ... Carl Zeiss
B 40-075 e 10/03 1-5
Key to Fig. 1-1
1 Stand
2 Eyepiece
3 Binocular tube 30°/23
4 HBO 100 W transformer
5 HBO 103 illuminator
6 HAL 12 V 100 W halogen lamp
7 FL filter slider
8 Objective
9 DIC slider
10 Compensator λ
11 Dust cover
12 DIC prism
13 Achromatic-aplanatic universal condenser 0.9 H D DIC
14 Filter mount
15 Mechanical stage 75x50 R
16 Specimen holder with spring clip R
17 FL P&C reflector module

INSTRUMENT DESCRIPTION Axioskop 2 plus
Carl Zeiss Axioskop 2 plus microscope configurations ... Axioskop 2 mot plus
1-6 B 40-075 e 10/03
Axioskop 2 plus (manual) microscope configurations Cat. No.
Configurations
The "Axioskop 2 plus" routine microscope is offered in the following different configurations:
for transmitted-light brightfield
with objectives "A-Plan" 5x, 10x, 20x, 40x and 100x
for transmitted-light brightfield
with objectives "Achroplan" 4x, 10x, 20x, 40x and 100x
for transmitted-light brightfield and phase contrast with
objectives "Achroplan" 4x, 10x Ph 1, 20x Ph2, 40x Ph 2 and 100x
for transmitted-light brightfield
with objectives "Plan-Neofluar" 2.5x, 10x, 20x, 40x and 100x
for transmitted-light brightfield and phase contrast
with objectives "Plan-Neofluar" 2.5x, 10x Ph 1, 20x Ph 2, 40x Ph 2 and 100x Ph 3
for transmitted-light brightfield and interference contrast
with objectives "Plan-Neofluar" 10x, 20x, 40x and 100x
for epi-fluorescence and transmitted-light phase contrast with objectives Plan-Neofluar/Plan-
Apochromat, Plan-Neofluar 2.5, 10x Ph 1, 20x Ph 2, 40x, Plan-Apochromat 63x and Plan-
Neofluar 100x
Modules
The "Axioskop 2 plus" routine microscope consists of the following modules:
Axioskop 2 plus microscope stand with 5x reflector turret, harmonic drive focusing drive,
5-position nosepiece H DIC W 0.8”, integrated 12 V DC 100 W power unit (stabilized),
transmitted-light equipment with filter magazine D, stage and condenser carrier,
FL incident light equipment and HBO/XBO adjusting aid
452310-0000-000
Eyepieces:
W-PL 10x/23 Br. foc.
PL 10x/23 Br. foc.
000000-1016-758
000000-1026-548
Tubes:
Binocular tube 30°/23
Binocular phototube 30°/23 (30/70)
Binocular ergotube 6-25°/23
Binocular ergo-phototube 6-25°/23 (100/0 or 0/100)
452340-0000-000
452344-0000-000
452341-0000-000
452342-0000-000
Illuminators:
HAL 12 V 100 W halogen illuminator
HBO 103 illuminator
HBO 100 W transformer for HBO 100 W/2 and HBO 103 W/2
447219-0000-000
487211-9804-000
458451-9901-000
Objectives see also section 0
DIC sliders:
DIC slider 10x/0.30 I
DIC slider 20x/0.50 II
DIC slider 40x/0.75 II
DIC slider 100x/1.30 III
444431-0000-000
444437-0000-000
444463-0000-000
444480-0000-000
Compensator λ, 6x20 473704-0000-000
DIC prisms with polarizer:
DIC prism I / 0.9
DIC prism II / 0.9
DIC prism III / 0.9
000000-1005-002
000000-1005-003
000000-1005-004