Agilent Technologies 1290 Infinity II User manual

Agilent Technologies
Agilent InfinityLab LC Series
1290 Infinity II
High Speed Pump
User Manual

1290 Infinity II High Speed Pump User Manual
Notices
© Agilent Technologies, Inc. 2014-2018
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Manual Part Number
G7120-90001 Rev. C
Edition
10/2018
Printed in Germany
Agilent Technologies
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76337 Waldbronn
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Safety Notices
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hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly per-
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notice until the indicated condi-
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or the like that, if not correctly
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cated conditions are fully
understood and met.

1290 Infinity II High Speed Pump User Manual 3
In This Guide…
In This Guide…
This manual covers the Agilent 1290 Infinity II High Speed Pump (G7120A).
1Introduction
This chapter gives an introduction to the pump and an instrument overview.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical
and performance specifications.
3UsingthePump
This chapter explains the operational parameters of the Agilent 1290 Infinity
II High Speed Pump.
4 Optimizing Performance
This chapter gives hints on how to optimize the performance or use additional
devices.
5 Troubleshooting and Diagnostics
Overview about the troubleshooting and diagnostic features.
6 Error Information
This chapter describes the meaning of error messages, and provides
information on probable causes and suggested actions how to recover from
error conditions.
7 Maintenance
This chapter describes the maintenance of the High Speed Pump.
8 Parts and Materials for Maintenance
This chapter provides information on parts for maintenance.

4 1290 Infinity II High Speed Pump User Manual
In This Guide…
9 Identifying Cables
This chapter provides information on cables used with the modules.
10 Hardware Information
This chapter describes the pump in more detail on hardware and electronics.
11 LAN Configuration
This chapter provides information on connecting the module to the Agilent
ChemStation PC.
12 Appendix
This chapter provides addition information on safety, legal and web.

1290 Infinity II High Speed Pump User Manual 5
Contents
Contents
1Introduction 9
Product Description 10
Pump Principle 11
Leak and Waste Handling 13
2 Site Requirements and Specifications 19
Site Requirements 20
Physical Specifications 23
Performance Specifications 24
3UsingthePump 27
Magnets 28
Turn on/off 29
Status Indicators 31
Best Practices 32
Purging the Pump 35
Solvent Information 37
Solvent Recommendation for Agilent 1290 Infinity and 1290 Infinity II and 1260
Infinity II Flexible Pumps 44
Algae Growth in HPLC Systems 49
Setting up the Pump with the Instrument Control Interface 50
Agilent Local Control Modules 60
4 Optimizing Performance 63
Delay Volume and Extra-Column Volume 64
How to Configure the Optimum Delay Volume 65
How to Achieve Higher Resolution 67
Using Solvent Calibration Tables 70

6 1290 Infinity II High Speed Pump User Manual
Contents
5 Troubleshooting and Diagnostics 71
User Interfaces 72
Agilent Lab Advisor Software 73
Pump Leak Rate Test 74
System Pressure Test 79
6 Error Information 81
What Are Error Messages 83
General Error Messages 84
Pump Error Messages 94
7 Maintenance 111
Introduction to Maintenance 112
Warnings and Cautions 114
Overview of Maintenance 116
Cleaning the Module 117
Install Fittings and Capillaries 118
Remove and Install Doors 119
Replace the Shutoff Valve Panel 121
Replace the Pressure Sensor 123
Replace the Solvent Selection Valve (SSV) 125
Change Configuration or Replace the Jet Weaver 127
Replace the Seal Wash Pump 130
Replace the Inlet Valve 131
Release a Stuck Inlet Valve 133
Remove the Pump Head Assembly 135
Pump Head Maintenance (Tool Free) 138
Install the Pump Head Assembly 153
Replace the Outlet Valve 155
Replace the Purge Valve Head 157
Replace Parts of the High Pressure Filter Assembly 160
Replace the Module Firmware 162
Prepare the Pump Module for Transport 163

1290 Infinity II High Speed Pump User Manual 7
Contents
8 Parts and Materials for Maintenance 167
Overview of Maintenance Parts 168
Flow Connections 170
Pump Heads 172
Purge Valve 177
Cover Parts 178
Accessory Kit 179
HPLC System Tool Kit 180
9 Identifying Cables 181
Cable Overview 182
Analog Cables 184
Remote Cables 186
CAN/LAN Cables 190
Agilent Module to PC 191
USB 192
10 Hardware Information 193
Firmware Description 194
Electrical Connections 197
Interfaces 199
Setting the 8-bit Configuration Switch 204
Early Maintenance Feedback 208
Instrument Layout 209
11 LAN Configuration 211
What You Have to Do First 212
TCP/IP parameter configuration 213
Configuration Switch 214
Initialization mode selection 215
Dynamic Host Configuration Protocol (DHCP) 217
Link configuration selection 220
Manual Configuration 221
PC and User Interface Software Setup Setup 226

8 1290 Infinity II High Speed Pump User Manual
Contents
12 Appendix 229
General Safety Information 230
Waste Electrical and Electronic Equipment (WEEE) Directive (2002-96-EC) 236
Radio Interference 237
Sound Emission 238
Agilent Technologies on Internet 239

9
1290 Infinity II High Speed Pump User Manual
Agilent Technologies
1
Introduction
Product Description 10
Pump Principle 11
Leak and Waste Handling 13
Leak Sensor 16
Waste Concept 17
This chapter gives an introduction to the pump and an instrument overview.

10 1290 Infinity II High Speed Pump User Manual
1 Introduction
Product Description
Product Description
The Agilent 1290 Infinity II High Speed Pump can enhance your efficiency
through high speed and chromatographic performance.
A low-delay-volume mixer allows you to run fast gradients for narrow-bore
applications for high laboratory efficiency.
The new 1290 Infinity II LC power range has a high instrument efficiency,
allowing you to run any HPLC and UHPLC method.
The full ISET range enables you to transfer existing methods from different
instruments, including current Agilent systems as well as instruments from
other manufacturers.
Active damping, automatic purge valve, new ultralow dispersion kits or low
delay-volume capability, combine to achieve high instrument and analytical
efficiency.
Figure 1 Overview of the High Speed Pump
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1290 Infinity II High Speed Pump User Manual 11
Introduction
1
Pump Principle
Pump Principle
The 1290 Inifinity II Highspeed Pump features a dual pump head design for
generation of binary gradients.
A solvent selection valve allows to choose from two solvents per pump head.
However, this valve cannot the switched during a run.
Each pump head is equpipped with two independently actuated pistons in
series.
Delivery cycle:
1Piston two moves forward to deliver solvent into the flow path. The
flow-rate is thereby determined by the speed of the piston.
At the same time, piston one draws solvent from the solvent reservoir. The
two piston chambers are isolated by a check valve (outlet ball valve).
2Shortly before the end of the delivery stroke of piston two, piston one
reverses its direction. The check valve (passive inlet valve) at the inlet to
piston chamber one closes while the solvent in piston chamber one gets
compressed to system operating pressure.
3Piston two reverses while piston one delivery the set flow rate into the flow
path and re-fills piston chamber two.
4When piston two has reached that end of its intake stroke it reverses and
the delivery cycle starts again with step 1)
Reproducible solvent properties are maintained by an integrated two-channel
solvent degasser. It is located between solvent selection valve and the pump
heads.
The pump automatically compensates for pressure- and flow instabilities
caused by the complex relationship between solvent compressibility and
system pressure.
The only user interaction is selecting the appropriate solvent or solvent
mixture per channel from a drop-down list.

12 1290 Infinity II High Speed Pump User Manual
1 Introduction
Pump Principle
Figure 2 The hydraulic path
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3UHVVXUHVHQVRU

1290 Infinity II High Speed Pump User Manual 13
Introduction
1
Leak and Waste Handling
Leak and Waste Handling
The Agilent InfinityLab LC Series has been designed for safe leak and waste
handling. It is important that all security concepts are understood and
instructions are carefully followed.
The solvent cabinet is designed to store a maximum volume of 8 L solvent. The
maximum volume for an individual bottle stored in the solvent cabinet should
not exceed 2 L. For details, see the usage guideline for the Agilent Infinity II
Solvent Cabinets (a printed copy of the guideline has been shipped with the
solvent cabinet, electronic copies are available on the Internet).
All leak plane outlets are situated in a consistent position so that all Infinity
and Infinity II modules can be stacked on top of each other. Waste tubes are
guided through a channel on the right hand side of the instrument, keeping the
front access clear from tubes.
The leak plane provides leak management by catching all internal liquid leaks,
guiding them to the leak sensor for leak detection, and passing them on to the
next module below, if the leak sensor fails. The leak sensor in the leak plane
stops the running system as soon as the leak detection level is reached.
Solvent and condensate is guided through the waste channel into the waste
container:
•from the detector's flow cell outlet
•from the Multisampler needle wash port
•from the Sample Cooler or Sample Thermostat (condensate)
•from the pump's Seal Wash Sensor (if applicable)
•from the pump's Purge Valve or Multipurpose Valve

14 1290 Infinity II High Speed Pump User Manual
1 Introduction
Leak and Waste Handling
Figure 3 Infinity II Leak Waste Concept (Flex Bench installation)

1290 Infinity II High Speed Pump User Manual 15
Introduction
1
Leak and Waste Handling
Figure 4 Infinity II Single Stack Leak Waste Concept (bench installation)

16 1290 Infinity II High Speed Pump User Manual
1 Introduction
Leak and Waste Handling
Figure 5 Infinity II Two Stack Leak Waste Concept (bench installation)
The waste tube connected to the leak pan outlet on each of the bottom
instruments guides the solvent to a suitable waste container.
Leak Sensor
CAUTION
Solvent incompatibility
The solvent DMF (dimethyl formamide) leads to corrosion of the leak sensor. The
material of the leak sensor, PVDF (polyvinylidene fluoride), is incompatible with DMF.
➔Do not use DMF.

1290 Infinity II High Speed Pump User Manual 17
Introduction
1
Leak and Waste Handling
Waste Concept
1Agilent recommends using the 6 L waste can with 1 Stay Safe cap GL45
with 4 ports (5043-1221) for optimal and safe waste disposal. If you decide
to use your own waste solution, make sure that the tubes don't immerse in
the liquid.

18 1290 Infinity II High Speed Pump User Manual
1 Introduction
Leak and Waste Handling

19
1290 Infinity II High Speed Pump User Manual
Agilent Technologies
2
Site Requirements and Specifications
Site Requirements 20
Physical Specifications 23
Performance Specifications 24
This chapter provides information on environmental requirements, physical
and performance specifications.

20 1290 Infinity II High Speed Pump User Manual
2 Site Requirements and Specifications
Site Requirements
Site Requirements
A suitable environment is important to ensure optimal performance of the
instrument.
Power Considerations
The module power supply has wide ranging capability. It accepts any line
voltage in the range described in Table 1 on page 23. Consequently there is no
voltage selector in the rear of the module. There are also no externally
accessible fuses, because automatic electronic fuses are implemented in the
power supply.
WARNING
Hazard of electrical shock or damage of your instrumentation
can result, if the devices are connected to a line voltage higher than specified.
➔Connect your instrument to the specified line voltage only.
WARNING
Electrical shock hazard
The module is partially energized when switched off, as long as the power cord is
plugged in.
The cover protects users from personal injuries, for example electrical shock.
➔Do not open the cover.
➔Do not operate the instrument and disconnect the power cable in case the cover
has any signs of damage.
➔Contact Agilent for support and request an instrument repair service.
WARNING
Inaccessible power plug.
In case of emergency it must be possible to disconnect the instrument from the
power line at any time.
➔Make sure the power connector of the instrument can be easily reached and
unplugged.
➔Provide sufficient space behind the power socket of the instrument to unplug the
cable.