Agilent Technologies 1200 series User manual

Agilent Technologies
Agilent 1200 Series
Preparative Pump
User Manual
Agilent 1200 Preparative Pump User Manual

Agilent 1200 Preparative Pump User Manual
Notices
© Agilent Technologies, Inc. 2006,
2007-2008
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Manual Part Number
G1361-90011
Edition
11/08
Printed in Germany
Agilent Technologies
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Not for use in Diagnostic Procedures.
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Safety Notices
CAUTION
A CAUTION notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
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result in damage to the product
or loss of important data. Do not
proceed beyond a CAUTION
notice until the indicated condi-
tions are fully understood and
met.
WARNING
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice,
or the like that, if not correctly
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WARNING notice until the indi-
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stood and met.

Agilent 1200 Preparative Pump User Manual 3
Contents
Contents
1 Introduction to the Preparative Pump 7
Introduction to the Preparative Pump 8
Instrument Layout 12
The Electronics 13
Electrical Connections 14
Agilent 1100/1200 Series Interfaces 16
2 Site Requirements and Specifications 23
Site Requirements 24
Physical Specifications 27
Performance Specifications 28
3 Installing the Pump 29
Unpacking the Preparative Pump 30
Optimizing the Stack Configuration 34
Installing the Preparative Pump 36
Connecting Modules and Control Software 40
Flow Connections for a Single (Isocratic) Preparative Pump 42
Flow Connections for a Dual (Binary Gradient) Preparative Pump 45
Get the System Ready for the First Analysis 49
4 Using the Preparative Pump 51
Hints for Successful Use of the Preparative Pump 52
Solvent Information 53
Prevent Blocking of Solvent Filters 54
Algae Growth in HPLC Systems 55
Setting Up the Pump 57
5 Optimizing Performance 65
How to Configure the Seal Wash Function 66
How to Optimize the Compressibility Compensation Setting 67

4 Agilent 1200 Preparative Pump User Manual
Contents
6 Troubleshooting and Diagnostics 69
Agilent Lab Advisor software 70
Overview of the Pump’s Indicators and Test Functions 71
Status Indicators 72
User Interfaces 74
Error Messages 75
Pressure Test 94
Leak Test 98
EMPV Cleaning 103
7 Maintenance 107
Introduction to Maintenance and Repair 108
Overview of Maintenance and Repair 112
Simple Repairs 114
8 Parts and Materials for Maintenance 133
Pump Housing and Main Assemblies 134
Pump-Head Assembly 138
Solvent Cabinet and Solvent Inlet Parts 140
Hydraulic Path 142
Preparative Pump Basis Kit 145
Preparative Pump Gradient Kit 147
9 Configuring the Preparative Pump 149
Preparative Pump Main Board (PPM) 150
Optional Interface Boards 155
Agilent 1200 Series Interfaces 159
Main Power Supply Assembly 164
10 Appendix 167
General Safety Information 168
The Waste Electrical and Electronic Equipment (WEEE) Directive
(2002/96/EC) 172
Lithium Batteries Information 173
Radio Interference 174
Sound Emission 175

Agilent 1200 Preparative Pump User Manual 5
Contents
Solvent Information 176
Agilent Technologies on Internet 178

6 Agilent 1200 Preparative Pump User Manual
Contents

7
Agilent 1200 Preparative Pump User Manual
Agilent Technologies
1
Introduction to the Preparative Pump
Introduction to the Preparative Pump 8
Hydraulic Path Overview 9
How Does Compressibility Compensation Work? 10
Early Maintenance Feedback (EMF) 11
Instrument Layout 12
The Electronics 13
Electrical Connections 14
Agilent 1100/1200 Series Interfaces 16

8 Agilent 1200 Preparative Pump User Manual
1Introduction to the Preparative Pump
Introduction to the Preparative Pump
Introduction to the Preparative Pump
The preparative pump is a cam driven pump, with fixed stroke and two
parallel pistons, each with (identical ball and seat) inlet and outlet valve. Each
of the two pump pistons is capable of delivering a maximum of 50 ml/min flow
at up to 400 bar pressure.
The two cams have overlapping delivery cycles. Compression losses are
calculated and smooth delivery is ensured through variation of the motor
speed over the pump cycle.
The pistons are guided through two seals each. The gap between those seals is
connected to capillary fittings, this way seal wash is not only an option, but
integral part of every pump. A peristaltic pump, to automate seal wash, is also
part of the system.
Figure 1 Overview of the Preparative Pump
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Agilent 1200 Preparative Pump User Manual 9
Introduction to the Preparative Pump 1
Introduction to the Preparative Pump
Hydraulic Path Overview
The pump flow leaving the pump head passes through the multi-assembly,
which combines the flow of the two pistons, filters the solvent and contains a
pressure sensor to monitor system pressure. No hydraulic damper is included
in the system. This makes very fast gradients possible. From the
multi-assembly the flow is routed to the purge valve and from there either
directly into waste or on to the next module (normally injection valve or
sampling device).
Gradients are formed with two combined pumps, as high pressure gradients.
The combined flow, is flowing through a passive mixer, to smooth composition.
With its high pressure mixing principle gradient systems and the usually high
flow rates, no degassing of solvents is necessary.
To avoid outgassing in the detector cell a back pressure regulating device is
recommended for applications that demand it.
Figure 2 Schematics of the Preparative Pump
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10 Agilent 1200 Preparative Pump User Manual
1Introduction to the Preparative Pump
Introduction to the Preparative Pump
For pump specifications, see “Site Requirements” on page 24.
How Does Compressibility Compensation Work?
The compressibility of the solvents in use will affect retention-time stability
when the back pressure in the system changes (for example, ageing of
column). In order to minimize this effect, the pump provides a compressibility
compensation feature which optimizes the flow stability according to the
solvent type. The compressibility compensation is set to a default value and
can be changed through the user interface.
Without a compressibility compensation the following will happen during a
stroke of the first piston. The pressure in the piston chamber increases and
the volume in the chamber will be compressed depending on backpressure and
solvent type. The volume displaced into the system will be reduced by the
compressed volume.
When a compressibility compensation value for the pump head is set, the
pump processor calculates a compensation volume that depends on the
system pressure and the selected compressibility value. The pump has a fixed
stroke. To compensate for compressibility losses, the speed of the piston
movement has to be varied during the different parts of each stroke.
Table 1 Preparative Pump Details
Materials in contact with mobile phase
Bottle head assembly glass frits, PTFE compounds, PEEK
Pump head SST, sapphire
Inlet/Outlet Valves SST, sapphire, ruby, PEEK
EMPV SST, ruby, sapphire, PEEK
Filter Cup SST
Filter Plate SST, PEEK
Pressure Sensor SST, PEEK
Capillaries SST

Agilent 1200 Preparative Pump User Manual 11
Introduction to the Preparative Pump 1
Introduction to the Preparative Pump
Early Maintenance Feedback (EMF)
The early maintenance feedback (EMF) feature monitors the usage of specific
components in the instrument, and provides feedback when the user-settable
limits have been exceeded. The visual feedback in the user interface provides
an indication that maintenance procedures should be scheduled.
For details on EMF counters and how to use them, see Agilent Lab Advisor.

12 Agilent 1200 Preparative Pump User Manual
1Introduction to the Preparative Pump
Instrument Layout
Instrument Layout
The industrial design of the module incorporates several innovative features.
It uses Agilent’s E-PAC concept for the packaging of electronics and
mechanical assemblies. This concept is based upon the use of expanded
polypropylene (EPP) layers foam plastic spacers in which the mechanical and
electronic boards components of the module are placed. This pack is then
housed in a metal inner cabinet which is enclosed by a plastic external
cabinet. The advantages of this packaging technology are:
•virtual elimination of fixing screws, bolts or ties, reducing the number of
components and increasing the speed of assembly/disassembly,
•the plastic layers have air channels molded into them so that cooling air can
be guided exactly to the required locations,
•the plastic layers help cushion the electronic and mechanical parts from
physical shock, and
•the metal inner cabinet shields the internal electronics from
electromagnetic interference and also helps to reduce or eliminate radio
frequency emissions from the instrument itself.

Agilent 1200 Preparative Pump User Manual 13
Introduction to the Preparative Pump 1
The Electronics
The Electronics
The electronics are comprised of four main components:
•The preparative pump main board (PPM), see “Preparative Pump Main
Board (PPM)” on page 150.
•Power supply, see “Main Power Supply Assembly” on page 164.
Optional:
•Interface board (BCD/external contacts), see “BCD / External Contact
Board” on page 155
•LAN Communication Card, see “LAN Communication Interface Board” on
page 157.
Main Board
The board controls all information and activities of all assemblies within the
module. The operator enters parameters, changes modes and controls the
module through interfaces (CAN, GPIB or RS-232C), connected to the
user-interfaces.
Main Power Supply Assembly
The main power supply comprises a closed assembly (no on-site repair
possibility). The power supply provides all DC voltages used in the module
except for the voltages supplied by the lamp power supply to the deuterium
and tungsten lamps in the detectors.
The line voltage can vary in a range from 100 – 240 volts AC ± 10% and needs
no manual setting.
Optional Interface Boards
The Agilent 1200 Series modules have one optional board slot that allows to
add an interface board to the modules. Optional interface boards for the
Agilent 1200 Series are:
•BCD Board
•LAN Communication Card

14 Agilent 1200 Preparative Pump User Manual
1Introduction to the Preparative Pump
Electrical Connections
Electrical Connections
•The GPIB connector is used to connect the module with a computer. The
address and control switch module next to the GPIB connector determines
the GPIB address of your module. The switches are preset to a default
address and is recognized once after power is switched ON.
•The CAN bus is a serial bus with high speed data transfer. The two
connectors for the CAN bus are used for internal Agilent 1200 Series
module data transfer and synchronization.
•One analog output provides signals for integrators or data handling
systems.
•The interface board slot is used for external contacts and BCD bottle
number output or LAN connections.
•The REMOTE connector may be used in combination with other analytical
instruments from Agilent Technologies if you want to use features such as
start, stop, common shut down, prepare, and so on.
•With the appropriate software, the RS-232C connector may be used to
control the module from a computer through a RS-232C connection. This
connector is activated and can be configured with the configuration switch.
See your software documentation for further information.
•The power input socket accepts a line voltage of 100 – 240 volts AC ± 10%
with a line frequency of 50 or 60 Hz. Maximum power consumption is
220 VA. There is no voltage selector on your module because the power
supply has wide-ranging capability. There are no externally accessible
fuses, because automatic electronic fuses are implemented in the power
supply. The security lever at the power input socket prevents the module
cover from being taken off when line power is still connected.
NOTE
Never use cables other than the ones supplied by Agilent Technologies to ensure proper
functionality and compliance with safety or EMC regulations.

Agilent 1200 Preparative Pump User Manual 15
Introduction to the Preparative Pump 1
Electrical Connections
Figure 3 Rear View of Preparative Pump - Electrical Connections and Label
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16 Agilent 1200 Preparative Pump User Manual
1Introduction to the Preparative Pump
Agilent 1100/1200 Series Interfaces
Agilent 1100/1200 Series Interfaces
The Agilent 1100/1200 Series modules provide the following interfaces:
Table 2 Agilent 1100/1200 Series Interfaces
Module CAN LAN/BCD
(optional)
LAN
(on-board)
GPIB RS-232 Analog APG
Remote
Special
Pumps
G1310A ISO
G1311A QUAT
G1312A BIN
G2226A NANO
2 Yes No Yes Yes 1 Yes
G1312B BIN SL 2 Yes No Yes Yes 1 Yes
G1361A PREP 2 Yes No No Yes No Yes CAN-DC- OUT for
CAN slaves
Samplers
G1313A STD 2 Yes No Yes Yes No Yes
G1329A STD
G1329B STD SL
G2260A PREP
2 Yes No Yes Yes No Yes THERMOSTAT for
G1330A/B
G1364A FRC
G1367A/B/C/D
WPS
G1377A µWPS
G2258A D-LOOP
2 Yes No Yes Yes No Yes THERMOSTAT for
G1330A/B
CAN-DC- OUT for
CAN slaves
Detectors
G1314A/B VWD 2 Yes No Yes Ye s 1 Yes
G1314C VWD SL 2 Yes No No Yes 1 Yes
G1314D VWD 2 No Yes No Yes 1 Yes
G1314E VWD SL+ 2 No Yes No Yes 1 Yes

Agilent 1200 Preparative Pump User Manual 17
Introduction to the Preparative Pump 1
Agilent 1100/1200 Series Interfaces
•CAN connectors as interface to other Agilent 1200 Series modules,
•GPIB connector as interface to the Agilent ChemStation,
•RS-232C as interface to a computer,
•REMOTE connector as interface to other Agilent products,
•analog output connector(s) for signal output, and
•interface slot for specific interfacing (external contacts, BCD, LAN and so
on).
For identification and location of the connectors, see the module manual.
G1315A/B DAD
G1365A/B MWD
2 Yes No Yes Yes 2 Yes
G1315C DAD SL
G1365C MWD SL
G1315D DAD
G1365D MWD
2 No Yes No Yes 2 Ye s
G1321A FLD
G1362A RID
2 Yes No Yes Yes 1 Yes
G4280A ELSD No No NO No Yes Yes Yes EXT Contact
AUTOZERO
Others
G1316A TCC No No No A Yes No Yes
G1316B TCC SL No No No A Yes No Yes
G1322A DEG No No No No No No Yes AUX
G1379A DEG No No No No Yes No No AUX
G4240A CHIP CUBE 2 Yes No No Yes No Yes CAN-DC- OUT for
CAN slaves
THERMOSTAT for
G1330A/B (NOT
USED
Table 2 Agilent 1100/1200 Series Interfaces
Module CAN LAN/BCD
(optional)
LAN
(on-board)
GPIB RS-232 Analog APG
Remote
Special

18 Agilent 1200 Preparative Pump User Manual
1Introduction to the Preparative Pump
Agilent 1100/1200 Series Interfaces
CAN
The CAN is inter-module communication interface. It is a 2-wire serial bus
system supporting high speed data communication and real-time requirement.
LAN
The 1100/1200 modules have either an interface slot for an LAN card (e.g.
Agilent G1369A LAN Interface) or they have an on-board LAN interface (e.g.
detectors G1315C/D DAD and G1365C/D MWD). This interface allows the
control of the module/system via a connected PC with the appropriate control
software (e.g. Agilent ChemStation).
GPIB
This interface is not available in all modules and may be removed from the
modules in future.
The GPIB connector is used to connect the module with a computer. The
address and control switches next to the GPIB connector determine the GPIB
address of your module. The switches are preset to a default address and
recognized by the operating software from Agilent Technologies.
RS-232C (Serial)
The RS-232C connector is used to control the module from a computer
through RS-232C connection, using the appropriate software. This connector
can be configured with the configuration switch module next to the GPIB
connector.
NOTE
Never use cables other than the ones supplied by Agilent Technologies to ensure proper
functionality and compliance with safety or EMC regulations.
NOTE
If a Agilent 1100/1200 series detector (DAD/MWD/FLD/VWD/RID) is in the system, the
LAN should be connected to the DAD/MWD/FLD/VWD/RID (due to higher data load). If
no Agilent detector is part of the system, the LAN interface should be installed in the pump
or autosampler.

Agilent 1200 Preparative Pump User Manual 19
Introduction to the Preparative Pump 1
Agilent 1100/1200 Series Interfaces
The RS-232C is designed as DCE (data communication equipment) with a
9-pin male SUB-D type connector. The pins are defined as:
Figure 4 RS-232 Cable
Analog Signal Output
The analog signal output (e.g. detector signal or pump pressure signal) can be
distributed to a recording device. For details refer to the description of the
main board of the module.
APG Remote
The APG Remote connector may be used in combination with other analytical
instruments from Agilent Technologies if you want to use features as common
shut down, prepare, and so on.
Remote control allows easy connection between single instruments or systems
to ensure coordinated analysis with simple coupling requirements.
The subminiature D connector is used. The module provides one remote
connector which is inputs/outputs (wired-or technique).
To provide maximum safety within a distributed analysis system, one line is
dedicated to SHUT DOWN the system’s critical parts in case any module
detects a serious problem. To detect whether all participating modules are
switched on or properly powered, one line is defined to summarize the
POWER ON state of all connected modules. Control of analysis is maintained
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20 Agilent 1200 Preparative Pump User Manual
1Introduction to the Preparative Pump
Agilent 1100/1200 Series Interfaces
by signal readiness READY for next analysis, followed by START of run and
optional STOP of run triggered on the respective lines. In addition PREPARE
and START REQUEST may be issued. The signal level are defined as:
•standard TTL levels (0 V is logic true, + 5 V is false)
•fan-out is 10,
•input load is 2.2 kOhm against + 5 V, and
•output are open collector type, inputs/outputs (wired-or technique).
NOTE
All common TTL circuits operate with a 5 volt power supply. A TTL signal is defined as
"low" or L when between 0 V and 0.8 V and "high" or H when between 2.0 V and 5 V (with
respect to the ground terminal).
Table 3 Remote Signal Distribution
Pin Signal Description
1 DGND Digital ground
2 PREPARE (L) Request to prepare for analysis (for example, calibration, detector
lamp on). Receiver is any module performing pre-analysis activities.
3 START (L) Request to start run / timetable. Receiver is any module
performing run-time controlled activities.
4 SHUT DOWN (L) System has serious problem (for example, leak: stops pump).
Receiver is any module capable to reduce safety risk.
5 Not used
6 POWER ON (H) All modules connected to system are switched on. Receiver is any
module relying on operation of others.
7 READY (H) System is ready for next analysis. Receiver is any sequence
controller.
8 STOP (L) Request to reach system ready state as soon as possible (for
example, stop run, abort or finish and stop injection). Receiver is any
module performing run-time controlled activities.
9 START REQUEST (L) Request to start injection cycle (for example, by start key on any
module). Receiver is the autosampler.