Agilent Technologies 1200 series User manual

Agilent Technologies
Agilent 1200 Series
Preparative Pump
User Manual
Agilent 1200 Preparative Pump User Manual

Agilent 1200 Preparative Pump User Manual
Notices
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2007-2008
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Manual Part Number
G1361-90011
Edition
11/08
Printed in Germany
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Not for use in Diagnostic Procedures.
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Agilent 1200 Preparative Pump User Manual 3
Contents
Contents
1 Introduction to the Preparative Pump 7
Introduction to the Preparative Pump 8
Instrument Layout 12
The Electronics 13
Electrical Connections 14
Agilent 1100/1200 Series Interfaces 16
2 Site Requirements and Specifications 23
Site Requirements 24
Physical Specifications 27
Performance Specifications 28
3 Installing the Pump 29
Unpacking the Preparative Pump 30
Optimizing the Stack Configuration 34
Installing the Preparative Pump 36
Connecting Modules and Control Software 40
Flow Connections for a Single (Isocratic) Preparative Pump 42
Flow Connections for a Dual (Binary Gradient) Preparative Pump 45
Get the System Ready for the First Analysis 49
4 Using the Preparative Pump 51
Hints for Successful Use of the Preparative Pump 52
Solvent Information 53
Prevent Blocking of Solvent Filters 54
Algae Growth in HPLC Systems 55
Setting Up the Pump 57
5 Optimizing Performance 65
How to Configure the Seal Wash Function 66
How to Optimize the Compressibility Compensation Setting 67

4 Agilent 1200 Preparative Pump User Manual
Contents
6 Troubleshooting and Diagnostics 69
Agilent Lab Advisor software 70
Overview of the Pump’s Indicators and Test Functions 71
Status Indicators 72
User Interfaces 74
Error Messages 75
Pressure Test 94
Leak Test 98
EMPV Cleaning 103
7 Maintenance 107
Introduction to Maintenance and Repair 108
Overview of Maintenance and Repair 112
Simple Repairs 114
8 Parts and Materials for Maintenance 133
Pump Housing and Main Assemblies 134
Pump-Head Assembly 138
Solvent Cabinet and Solvent Inlet Parts 140
Hydraulic Path 142
Preparative Pump Basis Kit 145
Preparative Pump Gradient Kit 147
9 Configuring the Preparative Pump 149
Preparative Pump Main Board (PPM) 150
Optional Interface Boards 155
Agilent 1200 Series Interfaces 159
Main Power Supply Assembly 164
10 Appendix 167
General Safety Information 168
The Waste Electrical and Electronic Equipment (WEEE) Directive
(2002/96/EC) 172
Lithium Batteries Information 173
Radio Interference 174
Sound Emission 175

Agilent 1200 Preparative Pump User Manual 5
Contents
Solvent Information 176
Agilent Technologies on Internet 178

6 Agilent 1200 Preparative Pump User Manual
Contents

7
Agilent 1200 Preparative Pump User Manual
Agilent Technologies
1
Introduction to the Preparative Pump
Introduction to the Preparative Pump 8
Hydraulic Path Overview 9
How Does Compressibility Compensation Work? 10
Early Maintenance Feedback (EMF) 11
Instrument Layout 12
The Electronics 13
Electrical Connections 14
Agilent 1100/1200 Series Interfaces 16

8 Agilent 1200 Preparative Pump User Manual
1Introduction to the Preparative Pump
Introduction to the Preparative Pump
Introduction to the Preparative Pump
The preparative pump is a cam driven pump, with fixed stroke and two
parallel pistons, each with (identical ball and seat) inlet and outlet valve. Each
of the two pump pistons is capable of delivering a maximum of 50 ml/min flow
at up to 400 bar pressure.
The two cams have overlapping delivery cycles. Compression losses are
calculated and smooth delivery is ensured through variation of the motor
speed over the pump cycle.
The pistons are guided through two seals each. The gap between those seals is
connected to capillary fittings, this way seal wash is not only an option, but
integral part of every pump. A peristaltic pump, to automate seal wash, is also
part of the system.
Figure 1 Overview of the Preparative Pump
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Agilent 1200 Preparative Pump User Manual 9
Introduction to the Preparative Pump 1
Introduction to the Preparative Pump
Hydraulic Path Overview
The pump flow leaving the pump head passes through the multi-assembly,
which combines the flow of the two pistons, filters the solvent and contains a
pressure sensor to monitor system pressure. No hydraulic damper is included
in the system. This makes very fast gradients possible. From the
multi-assembly the flow is routed to the purge valve and from there either
directly into waste or on to the next module (normally injection valve or
sampling device).
Gradients are formed with two combined pumps, as high pressure gradients.
The combined flow, is flowing through a passive mixer, to smooth composition.
With its high pressure mixing principle gradient systems and the usually high
flow rates, no degassing of solvents is necessary.
To avoid outgassing in the detector cell a back pressure regulating device is
recommended for applications that demand it.
Figure 2 Schematics of the Preparative Pump
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10 Agilent 1200 Preparative Pump User Manual
1Introduction to the Preparative Pump
Introduction to the Preparative Pump
For pump specifications, see “Site Requirements” on page 24.
How Does Compressibility Compensation Work?
The compressibility of the solvents in use will affect retention-time stability
when the back pressure in the system changes (for example, ageing of
column). In order to minimize this effect, the pump provides a compressibility
compensation feature which optimizes the flow stability according to the
solvent type. The compressibility compensation is set to a default value and
can be changed through the user interface.
Without a compressibility compensation the following will happen during a
stroke of the first piston. The pressure in the piston chamber increases and
the volume in the chamber will be compressed depending on backpressure and
solvent type. The volume displaced into the system will be reduced by the
compressed volume.
When a compressibility compensation value for the pump head is set, the
pump processor calculates a compensation volume that depends on the
system pressure and the selected compressibility value. The pump has a fixed
stroke. To compensate for compressibility losses, the speed of the piston
movement has to be varied during the different parts of each stroke.
Table 1 Preparative Pump Details
Materials in contact with mobile phase
Bottle head assembly glass frits, PTFE compounds, PEEK
Pump head SST, sapphire
Inlet/Outlet Valves SST, sapphire, ruby, PEEK
EMPV SST, ruby, sapphire, PEEK
Filter Cup SST
Filter Plate SST, PEEK
Pressure Sensor SST, PEEK
Capillaries SST