Agilent Technologies LC Series User manual

Agilent Technologies
Agilent InfinityLab LC Series
1260 Infinity II Isocratic-
and Quaternary Pumps
User Manual

Agilent InfinityLab LC Series 1260 Infinity II Isocratic- and Quaternary Pumps User Manual
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Manual Part Number
G7111-90000 Rev. C
Edition
08/2018
Printed in Germany
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Agilent InfinityLab LC Series 1260 Infinity II Isocratic- and Quaternary Pumps User Manual 3
In This Guide...
In This Guide...
This manual covers:
•Agilent 1260 Infinity II Isocratic Pump (G7110B),
•Agilent 1260 Infinity II Quaternary Pump VL (G7111A),
•Agilent 1260 Infinity II Quaternary Pump (G7111B), and
•Agilent 1260 Infinity II Bio-inert Pump (G5654A).
1Introduction
This chapter gives an introduction to the module, instrument overview and
internal connectors.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical
and performance specifications.
3UsingthePump
This chapter provides information for optimized usage of the module.
4 Optimizing Performance
This chapter gives hints on how to optimize the performance or use additional
devices.
5 Troubleshooting and Diagnostics
This chapter gives an overview about the troubleshooting and diagnostic
features and the different user interfaces.
6 Error Information
This chapter describes the meaning of error messages, and provides
information on probable causes and suggested actions how to recover from
error conditions.

4 Agilent InfinityLab LC Series 1260 Infinity II Isocratic- and Quaternary Pumps User Manual
In This Guide...
7 Test Functions and Calibration
This chapter describes the tests for the module.
8 Maintenance
This chapter describes the maintenance of the module.
9PartsforMaintenance
This chapter provides information on parts for maintenance.
10 Identifying Cables
This chapter provides information on cables used with the Agilent 1200
Infinity Series modules.
11 Hardware Information
This chapter describes the pump in more detail on hardware and electronics.
12 LAN Configuration
This chapter provides information on connecting the module to the Agilent
ChemStation PC.
13 Appendix
This chapter provides addition information on safety, legal and web.

Agilent InfinityLab LC Series 1260 Infinity II Isocratic- and Quaternary Pumps User Manual 5
Contents
Contents
1Introduction 9
Introduction to the Pump 10
G7110B Isocratic Pump 11
G7111A Quaternary Pump VL 13
G7111B Quaternary Pump 15
G5654A Bio-inert Pump 17
Overview of the Hydraulic Path 19
Leak and Waste Handling 27
2 Site Requirements and Specifications 33
Site Requirements 34
Physical Specifications 37
Performance Specifications 41
3UsingthePump 49
Magnets 50
Turn on/off 51
Status Indicators 53
Best Practices 54
Leak and Waste Handling 57
Hints for Successful Use of the Pump 59
Setting up the Pump with the G4208A Instant Pilot 60
Setting up the Pump with the Instrument Control Interface 61
Solvent Information 71
Algae Growth in HPLC Systems 81
Prevent Blocking of Solvent Filters 82
Normal Phase Applications 83
4 Optimizing Performance 85
Using the Degasser 86
Operational Hints for the Vacuum Degasser 87
Operational Hints for the Multi Channel Gradient Valve (MCGV) 88
When to use the Seal Wash Function 89
Choosing the Right Pump Seals 90
Optimize the Compressibility Compensation Setting 91

6 Agilent InfinityLab LC Series 1260 Infinity II Isocratic- and Quaternary Pumps User Manual
Contents
5 Troubleshooting and Diagnostics 93
User Interfaces 94
Agilent Lab Advisor Software 95
6 Error Information 97
What Are Error Messages 99
General Error Messages 100
Module Error Messages 106
7 Test Functions and Calibration 121
Introduction 122
System Pressure Test 123
Leak Rate Test 128
8 Maintenance 135
Introduction to Maintenance and Repair 136
Warnings and Cautions 137
Overview of Maintenance and Repair 138
Cleaning the Module 139
Remove and Install Doors 140
Checking and Replacing the Solvent Filter 141
Exchange the Passive Inlet Valve 142
Exchange the Outlet Valve 144
Exchange the Purge Valve Frit or the Purge Valve 146
Replace the O-Ring on the Purge Valve 150
Remove the Pump Head Assembly 153
Maintenance of a Pump Head Without Seal Wash Option 155
Maintenance of a Pump Head with Seal Wash Option 158
Reinstall the Pump Head Assembly 162
Seal Wear-in Procedure 165
Exchange the Multi-Channel Gradient Valve (MCGV) 166
Replace the Solvent Selection Valve (Isocratic Pump) 169
Exchange the Active Inlet Valve (AIV) or its Cartridge 171
Exchange the Seal Wash Cartridge 173
Replace Leak Handling System Parts 174
Replacing the Module Firmware 175

Agilent InfinityLab LC Series 1260 Infinity II Isocratic- and Quaternary Pumps User Manual 7
Contents
9 Parts for Maintenance 177
Accessory Kits 178
Pump Head Assembly Without Seal Wash 180
Pump Head Assembly with Seal Wash Option 182
Outlet Valve 185
Purge Valve Assembly 186
Active Inlet Valve Assembly 188
1260 Infinity II Max Uptime Kit 190
1260 Infinity II Max Uptime Kit Bio 191
1260 Infinity II Low Dispersion Kit MCT 192
HPLC System Tool Kit 193
Solvent Cabinet 194
Bottle Head Assembly 195
Hydraulic Path of the Quaternary Pump 196
Hydraulic Path of the Isocratic Pump 198
10 Identifying Cables 201
Cable Overview 202
Analog Cables 204
Remote Cables 206
CAN Cable 210
Agilent Module to PC 211
Agilent 1200 Module to Printer 212
11 Hardware Information 213
Firmware Description 214
Electrical Connections 217
Interfaces 219
Setting the 6-bit Configuration Switch 227
Instrument Layout 231
Early Maintenance Feedback 232
12 LAN Configuration 233
What You Have to Do First 234
TCP/IP parameter configuration 235
Configuration Switches 236
Initialization Mode Selection 237
Dynamic Host Configuration Protocol (DHCP) 239
Manual Configuration 242

8 Agilent InfinityLab LC Series 1260 Infinity II Isocratic- and Quaternary Pumps User Manual
Contents
13 Appendix 247
General Safety Information 248
Waste Electrical and Electronic Equipment (WEE) Directive 254
Radio Interference 255
Sound Emission 256
Agilent Technologies on Internet 257

9
Agilent InfinityLab LC Series 1260 Infinity II Isocratic- and Quaternary
Pumps User Manual
Agilent Technologies
1
Introduction
Introduction to the Pump 10
G7110B Isocratic Pump 11
Product Description 11
Features 12
G7111A Quaternary Pump VL 13
Product Description 13
Features 14
G7111B Quaternary Pump 15
Product Description 15
Features 16
G5654A Bio-inert Pump 17
Product Description 17
Features 18
Overview of the Hydraulic Path 19
Hydraulic Path 20
How Does the Pump Work? 22
How Does Compressibility Compensation Work? 25
How Does Variable Stroke Volume Work? 26
Leak and Waste Handling 27
Leak Sensor 31
Waste Concept 31
This chapter gives an introduction to the module, instrument overview and
internal connectors.

10 Agilent InfinityLab LC Series 1260 Infinity II Isocratic- and Quaternary Pumps User Manual
1 Introduction
Introduction to the Pump
Introduction to the Pump
The Pumps comprise one pump head unit with an optional solvent cabinet, a
vacuum degasser (optional for the isocratic pump), and a multi-channel
gradient valve (MCGV) for quaternary pumps, or an optional solvent selection
valve (SSV) for isocratic pumps. The combination of the pump head assembly
and high speed proportioning MCGV with a possible connection up to four
different solvents in quaternary pumps provides gradient generation by low
pressure mixing. The combination of the pump head assembly and SSV in
isocratic pump provides opportunity to make a choice between three different
solvents. A solvent cabinet provides enough space for eight one-liter bottles.
The Active Seal Wash (ASW) and Active Inlet Valve (AIV) options are available
for quaternary pumps when concentrated buffer solutions are used. The
Bio-inert pump is optimized for these conditions, thus is always equipped with
ASW and AIV.
Figure 1 Overview of the pump
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Agilent InfinityLab LC Series 1260 Infinity II Isocratic- and Quaternary Pumps User Manual 11
Introduction
1
G7110B Isocratic Pump
G7110B Isocratic Pump
Product Description
The Agilent 1260 Infinity II Isocratic Pump is ideal for demanding QA/QC
tasks and routine applications. It maintains virtually pulse-free and stable
solvent flows. The dual floating, precise servo-controlled pistons in the
delivery mechanism adjust the stroke volume according to your chosen flow
rate.
The optional integrated degasser and solvent selection valve offers increased
ease-of-use and method flexibility.
The pump can deliver a broad range of pressures up to a maximum 600 bar,
giving you the flexibility to use small particle size columns, longer columns or
alternative higher viscosity solvents.
The Agilent 1260 Infinity II Isocratic Pump is the ideal pump for GPC/SEC
applications where run-to-run and day-to-day precision in retention times is
crucial.
Figure 2 Overview of the pump
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/HDNGUDLQ

12 Agilent InfinityLab LC Series 1260 Infinity II Isocratic- and Quaternary Pumps User Manual
1 Introduction
G7110B Isocratic Pump
Features
•Higher resolution and increased throughput with the new standard
600 bar pressure for modern LC columns.
•Low operating and maintenance costs - due to excellent instrument
uptime.
•Future proof - your investment is protected since you can upgrade to a
quaternary pump if required.
•Versatile wide f low range – from 0.2 mL/min to 10 mL/min for analytical
and semi-preparative separations and purifications.
•Fast and easy maintenance - front access to all maintenance parts means
you can change components quickly without having to disassemble the
instrument.
•Longevity and low operating costs - robust materials (stainless steel,
titanium, gold, ruby, sapphire, ceramics, PEEK and PTFE) withstand the
most demanding applications and keep your pump functioning for years.
Our optional seal wash for high-salt mobile phases keeps maintenance to a
minimum and reduces operating costs.

Agilent InfinityLab LC Series 1260 Infinity II Isocratic- and Quaternary Pumps User Manual 13
Introduction
1
G7111A Quaternary Pump VL
G7111A Quaternary Pump VL
Product Description
The Agilent 1260 Infinity II Quaternary Pump VL has an extended power
range, delivering pressures up to 400 bar. At these high pressures you can use
smaller particle size columns and get higher resolution and faster separations.
It maintains virtually pulse-free, well mixed and stable solvent flows. Its dual
floating, precise servo-controlled pistons adjust the stroke volume according
to your chosen flow rate.
Offering access to up to four solvents, the Agilent 1260 Infinity II Quaternary
Pump VL provides the greatest flexibility in automated solvent blending and is
recommended for a wide range of research and routine applications
(especially in the food, environmental and pharmaceutical sectors) as well as
for method development.
Figure 3 Overview of the pump
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'HJDVVHU
0&*9

14 Agilent InfinityLab LC Series 1260 Infinity II Isocratic- and Quaternary Pumps User Manual
1 Introduction
G7111A Quaternary Pump VL
Features
•Higher resolution, faster separations - the maximum pressure of 400 bar
allows you to use smaller particle size columns, longer columns and/or
higher viscous solvents as an alternative to acetonitrile.
•Analytical and semi-preparative purifications - the wide flow range (up
to 10 mL/min) perfectly supports standard and semi-preparative
applications. The perfect choice for 4.6 mm and 3.0 mm id columns.
•Quicker experiments - the pump offers convenient access to up to four
solvents for isocratic or gradient analysis which can really speed up method
development, preparation of mobile phases and system flushing.
•Easy, fast and secure transfer of methods from HPLC to sub-2-micron
columns.
•Integrated degasser
•Longevity and low operating costs - robust materials (stainless steel,
titanium, gold, ruby, sapphire, ceramics, PEEK and PTFE) withstand the
most demanding applications and keep your pump functioning for years.
Our optional seal wash for high-salt mobile phases keeps maintenance to a
minimum and reduces operating costs.

Agilent InfinityLab LC Series 1260 Infinity II Isocratic- and Quaternary Pumps User Manual 15
Introduction
1
G7111B Quaternary Pump
G7111B Quaternary Pump
Product Description
The Agilent 1260 Infinity II Quaternary Pump has an extended power range,
delivering pressures up to 600 bar. At these high pressures you can use
smaller particle size columns and get higher resolution and faster separations.
It maintains virtually pulse-free, well mixed and stable solvent flows. Its dual
floating, precise servo-controlled pistons adjust the stroke volume according
to your chosen flow rate.
Offering access to up to four solvents, the Agilent 1260 Infinity II Quaternary
Pump provides the greatest flexibility in automated solvent blending and is
recommended for a wide range of research and routine applications
(especially in the food, environmental and pharmaceutical sectors) as well as
for method development.
Figure 4 Overview of the pump
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3XUJHYDOYH
3RZHUVZLWFK
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'HJDVVHU
0&*9

16 Agilent InfinityLab LC Series 1260 Infinity II Isocratic- and Quaternary Pumps User Manual
1 Introduction
G7111B Quaternary Pump
Features
•Higher resolution, faster separations - the maximum pressure of 600 bar
allows you to use smaller particle size columns, longer columns and/or
higher viscous solvents as an alternative to acetonitrile.
•Analytical and semi-preparative purifications - the wide flow range (up
to 10 mL/min) perfectly supports standard and semi-preparative
applications. The perfect choice for 4.6 mm and 3.0 mm id columns.
•Quicker experiments - the pump offers convenient access to up to four
solvents for isocratic or gradient analysis which can really speed up method
development, preparation of mobile phases and system flushing.
•Easy, fast and secure transfer of methods from HPLC to sub-2-micron
columns.
•Integrated degasser
•Longevity and low operating costs - robust materials (stainless steel,
titanium, gold, ruby, sapphire, ceramics, PEEK and PTFE) withstand the
most demanding applications and keep your pump functioning for years.
Our optional seal wash for high-salt mobile phases keeps maintenance to a
minimum and reduces operating costs.

Agilent InfinityLab LC Series 1260 Infinity II Isocratic- and Quaternary Pumps User Manual 17
Introduction
1
G5654A Bio-inert Pump
G5654A Bio-inert Pump
Product Description
The 1260 Infinity II Bio-Inert Pump is the pump of choice for all your
biological and extreme pH applications. The titanium-based pump offers
highest corrosion resistance against high salt concentration (2 M) and offers a
handling of a wide pH range (1 – 13, short term 14). It features a pressure
range of up to 600 bar and a flow rate up to 10 mL/min (at 200 bar). Which
enables the use of almost any column: conventional, sub-2 m particle, or
superficially porous columns.
Together with the Agilent Bio-HPLC column portfolio for SEC and IEX, the
highest resolution per time is achieved for protein and NBE characterization.
The Agilent Buffer Advisor software allows fast pH scouting and easy
buffer/solvent preparation in ion exchange chromatography.
Figure 5 Overview of the Bio-inert Pump
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18 Agilent InfinityLab LC Series 1260 Infinity II Isocratic- and Quaternary Pumps User Manual
1 Introduction
G5654A Bio-inert Pump
Features
•Titanium pump - for highest corrosion resistance against high salt
concentrations and harsh cleaning procedures
•Steel and iron-free wetted parts in solvent delivery unit - ensure the
integrity of bio-molecules and minimize unwanted surface interactions
while increasing column life-time
•High salt tolerance (2 M) and wide pH range (1 – 13, short term 14) - for
increased flexibility
•Flow rates up to 10 mL/min - enable power ranges from lowest pressure for
traditional bio-purification columns up to high pressure STM analytical
bio-columns
•Active seal wash and quaternary solvent blending included
•Comes with Agilent Buffer Advisor software - for faster pH scouting and
easy buffer/solvent preparation in ion exchange chromatography

Agilent InfinityLab LC Series 1260 Infinity II Isocratic- and Quaternary Pumps User Manual 19
Introduction
1
Overview of the Hydraulic Path
Overview of the Hydraulic Path
The Low Pressure Mixing Pumps are based on a two-channel, dual-piston
in-series design which comprises all essential functions that a solvent delivery
system has to fulfill. Metering of solvent and delivery to the high-pressure side
are performed by one pump assembly which can generate pressure up to
600 bar.
In the quaternary pumps, degassing of the solvents is done in a built-in
vacuum degasser. Solvent compositions are generated on the low-pressure
side by an MCGV.
The pump assembly includes a pump head with an inlet valve and an outlet
valve. A damping unit is connected between the two piston chambers. A purge
valve including a PTFE frit is fitted at the pump outlet for convenient priming
of the pump head.

20 Agilent InfinityLab LC Series 1260 Infinity II Isocratic- and Quaternary Pumps User Manual
1 Introduction
Overview of the Hydraulic Path
Hydraulic Path
Figure 6 Hydraulic path of the isocratic pump
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