Agilent Technologies 1260 Infinity User manual

Agilent Technologies
Agilent 1260 Infinity
Binary Pump
User Manual

1260 Infinity Binary Pump User Manual
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2010-2013
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Manual Part Number
G1312-90015 Rev. B
Edition
11/2013
Printed in Germany
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1260 Infinity Binary Pump User Manual 3
In This Guide...
In This Guide...
This manual covers the Agilent 1260 Infinity Binary Pump (G1312B).
1 Introduction to the Binary Pump
This chapter gives an introduction to the module and an instrument
overview.
2 Site Requirements and Specifications
This chapter provides information about site requirements and
specifications for the binary pump.
3 Installing the Pump
This chapter gives information about the preferred stack setup for your
system and the installation of your binary pump.
4UsingthePump
This chapter explains the operational parameters of the binary pump.
5 Optimizing Performance
This chapter gives information on how to optimize the performance of the
Binary Pump under special operational conditions.
6 Troubleshooting and Diagnostics
Overview of the troubleshooting and diagnostic features.
7 Error Information
This chapter describes the meaning of error messages, and provides
information on probable causes and suggested actions how to recover from
error conditions.

4 1260 Infinity Binary Pump User Manual
In This Guide...
8 Test Functions and Calibration
This chapter explains all test functions that are available for the binary
pump.
9 Diagnostic Signals
This chapter explains all diagnostic signals of the binary pump.
10 Maintenance
This chapter describes the maintenance of the module.
11 Parts and Materials for Maintenance
This chapter lists all parts and tools that are required for maintenance.
12 Identifying Cables
This chapter provides information on cables used with the Agilent 1200
Infinity Series modules.
13 Hardware Information
This chapter provides detailed technical information about your binary
pump.
14 Appendix
This appendix provides general safety and environmental information.

1260 Infinity Binary Pump User Manual 5
Contents
Contents
1 Introduction to the Binary Pump 9
Instrument and Operation 10
Overview of the Hydraulic Path 13
Leak and Waste Handling 18
2 Site Requirements and Specifications 21
Site Requirements 22
Physical Specifications 25
Performance Specifications 26
3 Installing the Pump 29
Unpacking the Binary Pump 30
Optimizing the Stack Configuration 33
Installation Information on Leak and Waste Handling 41
Installing the Binary Pump 45
Flow Connections with Solvent Selection Valve 48
Flow Connections without Solvent Selection Valve 51
Priming the System 54
4UsingthePump 59
Leak and Waste Handling 60
Hints for Successful Use of the Binary Pump 61
Setting up the Pump with the G4208A Instant Pilot 62
Setting up the Agilent 1260 Infinity Binary Pump G1312B with the Instrument
Control Interface 63
Solvent Information 69
Algae Growth in HPLC Systems 74
Prevent Blocking of Solvent Filters 76

6 1260 Infinity Binary Pump User Manual
Contents
5 Optimizing Performance 79
When to Use a Vacuum Degasser 80
When to Use the Active Seal Wash Option 81
When to Use Alternative Seals 82
When to Use the Low Volume Mixer 83
When to Remove Damper and Mixer 84
How to Optimize the Compressibility Compensation Setting 87
6 Troubleshooting and Diagnostics 91
Overview of the Module’s Indicators and Test Functions 92
Status Indicators 94
User Interfaces 96
Agilent Lab Advisor Software 97
7 Error Information 99
What Are Error Messages 101
General Error Messages 102
Module Error Messages 109
8 Test Functions and Calibration 125
(System) Pressure Test 126
Valve Test 131
Binary Pump Solvent Compressibility Calibration 133
Pump Elasticity Calibration 135
Leak Rate Test 137
9 Diagnostic Signals 143
Analog Pressure Output 144
Diagnostic Signals in Chromatographic Data Systems 145

1260 Infinity Binary Pump User Manual 7
Contents
10 Maintenance 147
Introduction to Maintenance and Repair 148
Warnings and Cautions 149
Cleaning the Module 151
Overview of Maintenance and Simple Repairs 152
Maintenance Procedures 153
Exchanging the Purge Valve Frit or the Purge Valve 154
Removing the Pump Head Assembly 157
Maintenance of a Pump Head without Seal Wash 159
Maintenance of a Pump Head with Seal Wash 163
Reinstalling the Pump Head Assembly 167
Seal Wear-in Procedure 169
Exchanging the Active Inlet Valve (AIV) or its Cartridge 170
Exchanging the Outlet Valve 173
Installation of the Solvent Selection Valve Upgrade Kit 175
Exchanging the Solvent Selection Valve 177
Exchanging the Optional Interface Board 180
Replacing Module Firmware 181
11 Parts and Materials for Maintenance 183
Hydraulic Path with Solvent Selection Valve 184
Hydraulic Path without Solvent Selection Valve 186
Pump Head Assembly Without Seal Wash 188
Pump Head Assembly with Seal Wash Option 190
Outlet Valve 192
Purge Valve Assembly 193
Active Inlet Valve Assembly 194
HPLC Starter Kit G4201-68707 195
HPLC Starter Kit G4202-68707 195
HPLC System Tool Kit 196
Bottle Head Assembly 197
Solvent Cabinet 198

8 1260 Infinity Binary Pump User Manual
Contents
12 Identifying Cables 201
Overview 202
Analog cables 204
Remote Cables 206
BCD Cables 209
CAN/LAN Cables 211
External Contact Cables 212
RS-232 Cable Kit 213
Agilent 1200 Module to Printer 214
13 Hardware Information 215
Firmware Description 216
Electrical Connections 219
Interfaces 221
Setting the 8-bit Configuration Switch (without On-board) LAN 228
Optional Interface Boards 232
The Solvent Selection Valve (SSV) 236
Early Maintenance Feedback 237
Instrument Layout 238
14 Appendix 239
General Safety Information 240
The Waste Electrical and Electronic Equipment Directive 243
Lithium Batteries Information 244
Radio Interference 245
Sound Emission 246
Agilent Technologies on the Internet 247

9
1260 Infinity Binary Pump User Manual
Agilent Technologies
1
Introduction to the Binary Pump
Instrument and Operation 10
Introduction to the Pump 10
Principle of Operation 11
Overview of the Hydraulic Path 13
Leak and Waste Handling 18
This chapter gives an introduction to the module and an instrument overview.

10 1260 Infinity Binary Pump User Manual
1Introduction to the Binary Pump
Instrument and Operation
Instrument and Operation
Introduction to the Pump
The binary pump comprises two identical pumps integrated into one
housing. Binary gradients are created by high- pressure mixing. An
optional degasser is available for applications that require best flow
stability, especially at low flow rates, for maximum detector sensitivity.
Pulse damper and mixer can be bypassed for low flowrate applications or
whenever a minimal transient volume is desirable. Typical applications are
high throughput methods with fast gradients on high resolution 2.1 mm
columns. The pump is capable of delivering flow in the range of 0.1 –
5 mL/min against up to 600 bar. A solvent selection valve (optional)
allows to form binary mixtures (isocratic or gradient) from one of two
solvents per channel. Active seal wash (optional) is available for use with
concentrated buffer solutions.
Figure 1 Overview of the binary pump
Purge valve
Active inlet valve
Mixer
Outlet valve