Agilent Technologies InfinityLab LC Series User manual

Agilent InfinityLab LC Series
Preparative Binary Pumps
User Manual
Preparative Binary Pumps User Manual

Preparative Binary Pumps User Manual
Notices
Manual Part Number
D0000602 Rev.B
EDITION 10/2019
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Preparative Binary Pumps User Manual 3
In This Guide...
This manual covers the following modules:
•Agilent 1260 Infinity II Preparative Binary Pump (G7161A)
•Agilent 1290 Infinity II Preparative Binary Pump (G7161B)
1Introduction
This chapter gives an introduction to the module and an instrument overview.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical and
performance specifications.
3 Using the Pump
This chapter explains the operational parameters of the module.
4 Optimizing Performance
This chapter gives hints on how to optimize the performance or use additional
devices.
5Troubleshootingand Diagnostics
Overview about the troubleshooting and diagnostic features.
6ErrorInformation
This chapter describes the meaning of error messages, and provides information
on probable causes and suggested actions how to recover from error conditions.
7 Maintenance
This chapter describes the maintenance of the module.

Preparative Binary Pumps User Manual 4
8 Parts and Materials for Maintenance
This chapter provides information on parts for maintenance.
9IdentifyingCables
This chapter provides information on cables used with the modules.
10 Hardware Information
This chapter describes the module in more detail on hardware and electronics.
11 LAN Configuration
This chapter provides information on connecting the module to the Agilent
ChemStation PC.
12 Appendix
This chapter provides addition information on safety, legal and web.

Preparative Binary Pumps User Manual 5
Contents
1Introduction9
G7161A Preparative Binary Pump 10
G7161B Preparative Binary Pump 12
Flow Reduction 14
Pump Principle 15
Leak and Waste Handling 18
2 Site Requirements and Specifications 19
Site Requirements 20
Physical Specifications 24
Performance Specifications 25
3 Using the Pump 31
Magnets 32
Turn on/off 33
Status Indicators 35
Best Practices 36
Purging the Pump 39
Solvent Information 41
Algae Growth in HPLC Systems 49
Agilent Local Control Modules 50
4 Optimizing Performance 51
Delay Volume and Extra-Column Volume 52
How to Achieve Higher Resolution 53
Using Solvent Calibration Tables 56
5Troubleshootingand Diagnostics 57
User Interfaces 58

Preparative Binary Pumps User Manual 6
Agilent Lab Advisor Software 59
Pump Leak Rate Test 60
6ErrorInformation64
What Are Error Messages 65
General Error Messages 66
Module Error Messages 73
7 Maintenance 79
Introduction to Maintenance 80
Warnings and Cautions 83
Overview of Maintenance 85
Cleaning the Module 86
Install Fittings and Capillaries 87
Remove and Install Doors 88
Open and Close Doors 90
Replace the Manifold 91
Replace the Y-Connector (G7161A) 93
Replace the Solvent Selection Valve (G7161B) 94
Replace the Solvent Mixer 96
Replace the Seal Wash Pump 97
Replace the Seal Wash Sensor 99
Replace the Purge Valve 101
Replace the Purge Valve Adapter and the Filter 103
Replace the Inlet Valve Cartridge 106
Release a Stuck Inlet Valve 108
Replace the Outlet Valve 110
Remove the Pump Head Assembly 112
Pump Head Maintenance 113
Install the Pump Head Assembly 117
Replace the Module Firmware 119
Prepare the Pump Module for Transport 120

Preparative Binary Pumps User Manual 7
8 Parts and Materials for Maintenance 122
Overview of Maintenance Parts (G7161A) 123
Overview of Maintenance Parts (G7161B) 124
Flow Connections (G7161A) 125
Flow Connections (G7161B) 126
Purge Valve 127
Pump Heads 128
Accessory Kit 131
HPLC System Tool Kit 132
9 Identifying Cables 133
Cable Overview 134
Analog Cables 136
Remote Cables 138
CAN/LAN Cables 142
Agilent Module to PC 143
USB 144
10 Hardware Information 145
Firmware Description 146
Electrical Connections 149
Interfaces 151
Setting the 6-bit Configuration Switch 158
Early Maintenance Feedback 160
Instrument Layout 161
11 LAN Configuration 162
What You Have to Do First 163
TCP/IP parameter configuration 164
Configuration Switches 165
Initialization Mode Selection 166

Preparative Binary Pumps User Manual 8
Dynamic Host Configuration Protocol (DHCP) 168
Manual Configuration 171
12 Appendix 175
General Safety Information 176
Waste Electrical and Electronic Equipment (WEEE) Directive 183
Radio Interference 184
Sound Emission 185
Agilent Technologies on Internet 186

Preparative Binary Pumps User Manual 9
1Introduction
G7161A Preparative Binary Pump 10
Product Description 10
Features 11
G7161B Preparative Binary Pump 12
Product Description 12
Features 13
Flow Reduction 14
Flow Reduction when Column Switching 14
Flow Reduction for Pump Protection (G7161B only) 14
Pump Principle 15
Leak and Waste Handling 18
Leak Sensor 18
This chapter gives an introduction to the module and an instrument overview.

Preparative Binary Pumps User Manual 10
1Introduction
G7161A Preparative Binary Pump
G7161A Preparative Binary Pump
Product Description
The 1260 Infinity II Preparative Binary Pump is an affordable high-pressure
gradient pump for LC purification. This pump has been designed and tested for
reverse-phase solvents and engineered for continuous use to provide reliable and
rugged performance through a dual-piston, rapid-refill design. It delivers flow
from 1 to 50 mL/min and is ideally suited for a broad range of semi-preparative
applications using conventional columns up to 30 mm id.
Figure 1 Overview of the pump
Status indicator
Purge valve
Leak and waste drain
Power switch
Active seal wash
Solvent mixer
Y-connector
Manifold
Channel B
Channel A

Preparative Binary Pumps User Manual 11
1Introduction
G7161A Preparative Binary Pump
Features
Purification efficiency
•Outstanding retention time stability for routine operation
Instrument efficiency
•Dynamic flow range up to 50 mL/min at 420 bar for semi-preparative
applications
•Outstanding binary gradient compositional accuracy across a wide dynamic
flow range
Laboratory efficiency
•Upper and lower pressure limits with automatic cutoff for increased safety in
the event of column blockage or leakage
•Automated seal wash for extended seal lifetime

Preparative Binary Pumps User Manual 12
1Introduction
G7161B Preparative Binary Pump
G7161B Preparative Binary Pump
Product Description
The 1290 Infinity II Preparative Binary Pump is an affordable high-pressure
gradient pump for LC purification. This pump has been designed and tested for
reverse-phase solvents and engineered for continuous use to provide reliable and
rugged performance through a dual-piston, rapid-refill design. It delivers flow
from 1 to 200 mL/min and is ideally suited for a broad range of semi-preparative
applications using conventional columns up to 50 mm id.
Figure 2 Overview of the pump
Active seal wash
Status indicator
Purge valve
Leak and waste drain
Power switch
Solvent mixer
Seal wash sensor
Solvent selection valve
Manifold
Channel B
Channel A

Preparative Binary Pumps User Manual 13
1Introduction
G7161B Preparative Binary Pump
Features
Purification efficiency
•Outstanding retention time stability for routine operation
Instrument efficiency
•Exchangeable pump heads deliver maximum efficiency and performance
across the entire flow range
•Dynamic flow range up to 50 mL/min at 600 bar using the analytical to
preparative pump head
•Dynamic flow range up to 200 mL/min using the preparative pump head
•Outstanding binary gradient compositional accuracy across a wide dynamic
flow range
Laboratory efficiency
•Upper and lower pressure limits with automatic cutoff for increased safety in
the event of column blockage or leakage
•Built-in active seal-wash for increased uptime

Preparative Binary Pumps User Manual 14
1Introduction
Flow Reduction
Flow Reduction
Flow Reduction when Column Switching
In a pressure cluster hosted by a Preparative Binary Pump, the flow reduction
feature protects the instrument and column from pressure spikes caused by a
switching valve by reducing the flow rate before switching the valve.
The flow reduction sequence is as follows:
1A valve switch command is issued by the user or method.
2The flow rate is reduced to zero mL/min at the rate defined in the Flow ramp
down setting (default: 600 mL/min/min) of the active method.
3When the flow rate reaches zero mL/min the pump sends a signal to the valve
that it is safe to switch.
4The valve switches and sends a signal to the pump when it is at its new
position.
5The flow rate is increased to the current set point defined in the Flow ramp up
setting (default: 600 mL/min/min) of the active method.
Flow Reduction for Pump Protection (G7161B only)
The flow reduction feature protects the pump against running dry at high flow for
extended periods.
The protection mechanism is triggered if the flow rate is 150 mL/min or higher
and the pressure is below 20 bar for 1 min. The flow rate is then reduced to
50.00 mL/min, but the solvent composition remains unchanged. The status LED
on the pump is yellow while the flow reduction is in force. In the driver dashboard,
the status bar is yellow (Not Ready), and the tooltip of the status bar displays Flow
Limit. The extended module tile in the dashboard displays the source of the limit
as Protect.
To clear the flow reduction feature, either set the flow rate for the respective
channel to 50 mL/min or less, or turn the pump off.
NOTE
The time taken to complete the valve switch will be increased if lower flow ramp
values are used.

Preparative Binary Pumps User Manual 15
1Introduction
Pump Principle
Pump Principle
Pump Principle of the 1260 Infinity II Preparative Binary Pump (G7161A): The
solvent flow leaving the two pump heads on each channel is combined by a
t-piece before passing through the manifold, which combines the flow from both
pump channels. From the manifold, the combined flow is routed to a pressure
sensor, then into the purge valve from which the flow goes through the mixer and
onto the next module (normally injection valve or sampling device).
Pump Principle of the 1290 Infinity II Preparative Binary Pump (G7161B): The
solvent flow leaving each pump head is combined by a t-piece, then passed
through a pressure sensor and then into a purge valve before being combined at
the manifold, which combines the flow from both channels. The flow then passes
through the mixer and onto the next module (normally injection valve or sampling
device).
Gradients are formed by the pump by combining the flow from both pump head
channels at high pressure, minimizing the need for degassing. To avoid
outgassing in the detector-cell, a back pressure regulating device is
recommended for applications that demand it.

Preparative Binary Pumps User Manual 16
1Introduction
Pump Principle
Figure 3 Hydraulic path of the pump (G7161A)
Seal wash pump
Y-connector
Purge valve Mixer
To sampler
Seal wash solvent
Manifold
Pressure
sensor
Pump head
AL
Pump head
AR
Pump head
BL
Pump head
BR

Preparative Binary Pumps User Manual 17
1Introduction
Pump Principle
Figure 4 Hydraulic path of the pump (G7161B)
Seal wash pump
Manifold
Purge valve
Mixer
Seal wash
solvent
Pump head
Pump head
Pump head
Pump head
AL
AR BR
BL
Pressure
sensor
Seal wash sensor
Solvent selection
valve
SamplerSolvent

Preparative Binary Pumps User Manual 18
1Introduction
Leak and Waste Handling
Leak and Waste Handling
1The figure below shows the leak and waste drain outlets of the module.
Leak Sensor
NOTE
Each module must have its own waste line. Waste lines cannot be combined.
CAUTION
Solvent incompatibility
The solvent DMF (dimethyl formamide) leads to corrosion of the leak sensor.
The material of the leak sensor, PVDF (polyvinylidene fluoride), is incompatible
with DMF.
Do not use DMF.

Preparative Binary Pumps User Manual 19
2 Site Requirements and Specifications
Site Requirements 20
Physical Specifications 24
Performance Specifications 25
Performance Specifications (G7161A) 25
Performance Specifications (G7161B) 28
This chapter provides information on environmental requirements, physical and
performance specifications.

Preparative Binary Pumps User Manual 20
2 Site Requirements and Specifications
Site Requirements
Site Requirements
A suitable environment is important to ensure optimal performance of the
instrument.
Power Considerations
The module power supply has wide ranging capability. It accepts any line voltage
in the range described in Table 1 on page 24. Consequently there is no voltage
selector in the rear of the module. There are also no externally accessible fuses,
because automatic electronic fuses are implemented in the power supply.
WARNING
Hazard of electrical shock or damage of your instrumentation
can result, if the devices are connected to a line voltage higher than
specified.
Connect your instrument to the specified line voltage only.
WARNING
Electrical shock hazard
The module is partially energized when switched off, as long as the power
cord is plugged in.
The cover protects users from personal injuries, for example electrical
shock.
Do not open the cover.
Do not operate the instrument and disconnect the power cable in case
the cover has any signs of damage.
Contact Agilent for support and request an instrument repair service.
WARNING
Inaccessible power plug.
In case of emergency it must be possible to disconnect the instrument
from the power line at any time.
Make sure the power connector of the instrument can be easily reached
and unplugged.
Provide sufficient space behind the power socket of the instrument to
unplug the cable.