Beckman Coulter Avanti J-26 XP Series User manual

J326XP-IM-5AB
January 2012
Instruction For Use
Avanti J-26XP
High-Performance Centrifuge
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821

Avanti J-26XP
High-Performance Centrifuge
J326XP-IM-5AB (January 2012)
Copyright © 2012 Beckman Coulter, Inc.
All rights reserved. No part of this document may be
reproduced or transmitted in any form or by any means,
electronic, mechanical, photocopying, recording, or
otherwise, without prior written permission from
Beckman Coulter, Inc.
Find us on the World Wide Web at:
www.beckmancoulter.com

J326XP-IM-5AB iii
Safety Notice
Read all product manuals and consult with Beckman Coulter-trained personnel before attempting
to operate the instrument. Do not attempt to perform any procedure before carefully reading all
instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as
to how to proceed in any situation, contact your Beckman Coulter Representative.
Alerts for Danger, Warning, Caution, and Note
DANGER
DANGER indicates an imminently hazardous situation which, if not avoided,
will result in death or serious injury.
WARNING
WARNING indicates a potentially hazardous situation which, if not avoided,
could result in death or serious injury.
CAUTION
CAUTION indicates a potentially hazardous situation, which, if not avoided,
may result in minor or moderate injury. It may also be used to alert against unsafe
practices.
NOTE NOTE is used to call attention to notable information that should be followed during installation, use,
or servicing of this equipment.
Safety During Installation and/or Maintenance
This instrument is designed to be installed by a Beckman Coulter Field Service representative.
Installation by anyone other than authorized Beckman Coulter personnel invalidates any warranty
covering the instrument. Also, if the instrument needs to be moved, a Beckman Coulter Field Service
representative must reinstall and relevel the instrument in its new location.
Any servicing of this equipment that requires removal of any covers can expose parts which involve
the risk of electric shock or personal injury. Make sure that the power switch is turned off an the
instrument is disconnected from the main power source, and refer such servicing to qualified
personnel.
Be sure to use the anchoring system to secure the centrifuge in place. The anchoring system is
designed to reduce the possibility of injury or damage that could result from instrument movement
in the event of a major rotor mishap.
Do not replace any centrifuge components with parts not specified for use on this instrument.

J326XP-IM-5AB
iv
Safety Notice
Electrical Safety
Electrical Safety
To reduce the risk of electrical shock, this instrument uses a three or five-wire electrical cord and
plug to connect this equipment to earth-ground. To preserve this safety feature:
• Make sure that the matching wall outlet receptacle is properly wired and earth-grounded.
Check that the line voltage agrees with the voltage listed on the name-rating plate affixed to the
centrifuge.
• Never use a three-to-two wire plug adapter.
• Never use a two-wire extension cord or a two-wire non-grounding type of multiple-outlet
receptacle strip.
Do not place containers holding liquid on or near the chamber door. If they spill, liquid may get into
the instrument and damage electrical or mechanical components.
Safety Against Risk of Fire
This instrument is not designed for use with materials capable of developing flammable or
explosive vapors.Do not centrifuge such materials (for example, chloroform or ethyl alcohol) in this
instrument nor handle or store them near the centrifuge.
Mechanical Safety
For safe operation of the equipment, observe the following:
• Use only the Beckman Coulter rotors and accessories designed for use in this instrument.
• Do not exceed the maximum rated speed of the rotor in use.
• NEVER attempt to slow or stop a rotor by hand.
• Do not lift or move the centrifuge while a rotor is spinning.
• NEVER attempt to override the door interlock system while the rotor is spinning.
• Do not lean on the centrifuge or place items on it while it is operating.
Chemical and Biological Safety
Normal operation may involve the use of solutions and test samples that are pathogenic, toxic, or
radioactive. Such materials should not be used in this instrument, however, unless all necessary
safety precautions are taken.
• Handle body fluids with care because they can transmit disease. No known test offers complete
assurance that they are free of micro-organisms. Some of the most virulent—Hepatitis (B and
C) and HIV (I–V) viruses, atypical mycobacteria, and certain systemic fungi—further emphasize
the need for aerosol protection. Handle other infectious samples according to good laboratory

J326XP-IM-5AB v
Safety Notice
Chemical and Biological Safety
procedures and methods to prevent spread of disease. Because spills may generate aerosols,
observe proper safety precautions for aerosol containment. Do not run toxic, pathogenic, or
radioactive materials in this centrifuge without taking appropriate safety precautions. Biosafe
containment should be used when Risk Group II materials (as identified in the World Health
Organization Laboratory Biosafety Manual) are handled; materials of a higher group require
more than one level of protection.
• Dispose of all waste solutions according to appropriate environmental health and safety
guidelines.
It is your responsibility to decontaminate the instrument and accessories before requesting service
by Beckman Coulter Field Service.

J326XP-IM-5AB
vi
Safety Notice
Chemical and Biological Safety

vii
Contents
Safety Notice,iii
Alerts for Danger, Warning, Caution, and Note,iii
Safety During Installation and/or Maintenance,iii
Electrical Safety,iv
Safety Against Risk of Fire,iv
Mechanical Safety,iv
Chemical and Biological Safety,iv
Introduction,xiii
Certification,xiii
Scope of This Manual, xiii
Conventions,xiv
CFC-Free Centrifugation,xiv
Radio Interference,xiv
Recycling Label,xv
CHAPTER 1: Specifications,1-1
Control Features,1-1
Operational Features,1-2
Physical Data,1-2
Available Rotors,1-3
Preinstallation Requirements,1-7
Electrical Requirements,1-8
Space and Location Requirements,1-11
Securing the Centrifuge to the Floor,1-11
Using J2 Series Rotors in the Avanti J,1-13
Checking for Rotor Drive Pins,1-13
Using the JA-18 Rotor,1-14
Using the JCF-Z Continuous Flow/Zonal Rotor,1-14

viii
Contents
CHAPTER 2: Centrifuge Function and Safety Features,2-1
Centrifuge Function,2-1
Safety Features,2-2
Housing and Door,2-2
Rotor Chamber,2-2
Drive,2-2
Friction Reduction System (FRS),2-3
Temperature Sensing and Control,2-3
Overtemp System,2-3
Name Rating Plate,2-3
Controls and Indicators,2-3
Power Switch,2-3
Control Panel,2-4
CHAPTER 3: Introduction,3-1
Summary of Avanti J-26XP Run Procedures,3-2
Section and Loading,3-3
Entering Run Parameters,3-4
Selecting a Rotor,3-4
Setting Run Speed,3-4
Setting Run Time,3-5
Setting Run Temperature,3-7
Setting Acceleration and Deceleration Rates,3-7
Setting Up an Elutriation Run,3-9
Starting a Run,3-11
Changing Parameters During a Run,3-12
Changing Time Settings,3-12
Stopping a Run,3-12
Unloading the Centrifuge,3-13
CHAPTER 4: Introduction,4-1
User Messages,4-1
Accessing the Rotor in Case of Power Failure,4-5
JCF-Z Rotor Identification,4-9
CHAPTER 5: Introduction,5-1
Maintenance,5-1
Cleaning,5-2
Decontamination,5-3
Sterilization and Disinfection,5-3
Replacing the Air Filter,5-4
Circuit Breaker and Fuses,5-5

ix
Contents
Storage and Transport,5-5
Storage,5-5
Returning a Centrifuge,5-5
Supply List,5-6
Replacement Parts and Supplies,5-6
APPENDIX A: Introduction,A-1
Avanti® J Series Centrifuge Warranty,Warranty-1

x
Illustrations
1.1 Single-Phase Electrical Requirements,1-9
1.2 Three-Phase “Y” Electrical Requirements,1-9
1.3 Correct and Incorrect Three-Phase Power Configurations,1-10
1.4 Rear View and Dimensions,1-12
1.5 Checking the Rotor for Drive Pins,1-13
2.1 The Power Switch,2-4
2.2 The Control Panel,2-4
3.1 Acceleration and Deceleration Settings Graph,3-8
3.2 Elutriation Controls,3-9
4.1 Emergency Door Release Latch Access,4-5
4.2 Manual Door Release,4-6
4.3 Venting the Chamber Vacuum,4-8
4.4 Front Panel Retaining Strip,4-9

xi
Tables
Tables
1.1 Nominal Supply Voltage Ratings for the Avanti J-26XP,1-8
1.2 Required Wire Connections,1-10
3.1 Acceleration Settings,3-7
3.2 Deceleration Settings,3-8
4.1 Diagnostic Message Chart,4-2

xii
Tables

J326XP-IM-5AB xiii
Introduction
Certification
To ensure full system quality, Beckman Coulter Avanti® J-26XP centrifuges are manufactured in a
registered ISO 9001 or 13485 facility. They have been designed and tested to be compliant (when
used with Beckman Coulter rotors) with the laboratory equipment requirements of applicable
regulatory agencies. Declarations of conformity and certificates of compliance are available at
www.beckmancoulter.com.
Scope of This Manual
This manual is designed to familiarize centrifuge users and site engineers with the Avant J-26XP
centrifuge, its functions, specifications, operation, and routine care and maintenance. We
recommend that you read this entire manual, especially the Safety Notice and all safety-related
information, before operating the instrument or performing instrument maintenance.
• CHAPTER 1, Specifications and Preinstallation Requirements contains system specifications and
instructions for preparing your site for centrifuge installation.
• CHAPTER 2, Description contains an overall description of the centrifuge, including a
description of system controls and indicators.
• CHAPTER 3, Operation summarizes procedures for operating the centrifuge.
• CHAPTER 4, Troubleshooting lists system diagnostic messages, together with probable causes
and user-performed corrective actions.
• CHAPTER 5, Care and Maintenance contains procedures for routine maintenance, as well as a
brief list of supplies and replacement parts.
• APPENDIX A contains a procedure to use when temperature control within ±1°C is required.
NOTE If the centrifuge is used in a manner other than specified in this manual, the safety and performance
of this equipment could be impaired. Further, the use of any equipment other than that recommended
by Beckman Coulter has not been evaluated for safety. Use of any equipment not specifically
recommended in this manual is the sole responsibility of the user.

J326XP-IM-5AB
xiv
Introduction
Conventions
Conventions
Certain symbols are used in this manual to call out safety related and other important information.
These international symbols may also be displayed on the centrifuge and are reproduced on the
inside back cover of the manual.
Typographic Conventions
Certain typographic conventions are used throughout this manual to distinguish names of user
interface components, such as keys and displays.
• Control knob names (for example, ROTOR and SPEED) appear in bold type.
• Key names (for example, START and STOP) appear in bold type.
• Display field names (for example, TEMP°C or SPEED) appear in bold type.
CFC-Free Centrifugation
To ensure minimal environmental impact, no CFCs are used in the manufacture or operation of the
Avanti J-26XP centrifuge.
Radio Interference
This instrument has been tested and found to comply with the limits for a Class A digital device,
pursuant to Part 15 of FCC Rules. These limits are designed to provide reasonable protection against
harmful interference when the equipment is operated in a commercial environment. This
equipment generates, uses, and can radiate radio frequency energy and, if not installed and used
in accordance with this instruction manual, may cause interference to radio communications.
Operation of this equipment in a residential area may cause interference, in which case the user
will be required to correct the interference at his own expense.
Canadian Regulations
This digital apparatus does not exceed the Class A limits for radio noise emissions from digital
apparatus as set out in the radio interference regulations of the Canadian Department of
Communications.
Le présent appareil numérique n’émet pas de bruits radioélectriques dépassant les limites
applicables aux appareils numériques de Classe A prescrites dans le reglement sur le brouillage
radioelectrique édicté par le Ministère des Communications du Canada.

J326XP-IM-5AB xv
Introduction
Recycling Label
Recycling Label
This symbol is required in accordance with the Waste Electrical and Electronic Equipment (WEEE)
Directive of the European Union. The presence of this marking on the product indicates:
1. The device was put on the European market after August 13, 2005 and
2. The device is not to be disposed via the municipal waste collection system of any member state
of the European Union.
It is very important that customers understand and follow all laws regarding the proper
decontamination and safe disposal of electrical equipment. For Beckman Coulter products bearing
this label please contact your dealer or local Beckman Coulter office for details on the take back
program that will facilitate the proper collection, treatment, recovery, recycling and safe disposal
of the device.
A28219-AA

J326XP-IM-5AB
xvi
Introduction
Recycling Label

J326XP-IM-5AB 1-1
CHAPTER 1
Specifications and Preinstallation Requirements
Specifications
Only values with tolerances or limits are guaranteed data. Values without tolerances are
informative data, without guarantee.
Control Features
Specifications Description
Speed • Setting range:
—100 to 26 000 rpm (in 100-rpm increments below 10 000 rpm,
500-rpm increments above 10 000 rpm), or equivalent RCF (in
100 gincrements)
• Elutriation speed setting range:
—0 to 5 000 rpm (in 10-rpm or 10 g increments)
• Speed display:
—from 0 to 10 000 rpm, display indicates actual rotor speed 10 rpm;
from 10 000 to 26 000 rpm, indicates actual rotor speed 0.1%, or
equivalent RCF
Time • Setting range:
—to 180 minutes or continuous (hold)
• Time display:
—Indicates time remaining (timed run) or time elapsed (hold run)
Temperature • Setting range:
—–10 to +40°C (in 1°C increments)
• Accuracy:
—rotor temperature controlled to within 2°C of set temperature (after
equilibration)a
• Ambient temperature range:
—16 to 38° (60 to 100°F)b
• Cooling fluid:
—refrigerant 404A (HFC)

J326XP-IM-5AB
1-2
Specifications and Preinstallation Requirements
Specifications
Operational Features
Physical Data
Acceleration maximum or slow
Deceleration maximum, slow, or off
a. During transient conditions, such as acceleration and deceleration, rotor temperature may be outside this range. Refer to
applicable rotor manuals for specific rotor operating range information.
b. To reach temperatures above ambient, the centrifuge is dependent on the frictional heat generated inside the chamber
during operation. At low run speeds or low ambient temperatures, the centrifuge may not be able to achieve some higher
temperatures.
Specifications Description
Specifications Description
Door 6.1-cm (2.4-in.) thick structural foam with steel plate
Rotor Chamber Diameter 51.3 cm (20 in.)
Friction Reduction
System (FRS)
190 mm (7.5 in.) Hg
Specification Description
Width 71 cm (28 in.)
Depth • 86 cm (34 in.)
• including air diverter extending from back panel
—102 cm (40.25 in.)
Height • with door closed
—86 cm (34 in.)
• to top of control head
—116 cm (45.5 in.)
• to top of open door
—149 cm (58.7 in.)
Weight 290 kg (640 lb)
Clearances
(for adequate
ventilation)
• sides
—7.6 cm (3 in.)
• back (place air diverter against the wall)
—16 cm (6.25 in.)
Surface Finish polyester control panel with polycarbonate coating overlay; polyurethane
enamel on door and covers; acrylic baking enamel on other surfaces

J326XP-IM-5AB 1-3
Specifications and Preinstallation Requirements
Available Rotors 1
Available Rotors
Refer to the applicable rotor manual for complete rotor descriptions.
Electrical Requirements • 200/208/240-V, single-phase instrument
—180–264 VAC, 30 A, 50/60 Hz
• 230-V, single-phase instrument
—180–264 VAC, 30 A, 50 Hz
• 220/380-V plus neutral, three-phaseainstrument
—313–457 VAC plus neutral, 16 A, 50 Hz
Electrical Supply Class I
Maximum Heat
dissipation into Room
Under Steady-State
Conditions
2.0 kW (6900 BTu/h)
Humidity restrictions <95% (noncondensing)
Noise Level 0.91 m (3 ft)
in front of instrument at
26 000 rpm
57 dBa
Installation
(overvoltage) Category
II
Pollution Degree 2b
a. Unbalanced three-phase. Split for single-phase operation internally.
b. Normally, only nonconductive pollution occurs; occasionally, however, a temporary conductivity caused by condensation
must be expected.
Specification Description
Rotor Profile Description
Rotor
Code
Max
RPMa
Max
RCF
(g) Max Capacity
Rotor
Manual
Number
JA-30.50 Ti b
Fixed Angle, 34°
(8 place)
rmax = 108 mm
30.50 26 000c81 800 8 50 mL J-TB-070
JA-25.50 Ti
Fixed Angle, 34°
(8 place)d
rmax = 108 mm
25.50 25 000 75 600 8 50 mL J-TB-056

J326XP-IM-5AB
1-4
Specifications and Preinstallation Requirements
Available Rotors
JA-25.15 Ti
Fixed Angle, 25°
(24 place)d
rmax = 106 mm
(outer row)
rmax = 86 mm
(inner row)
25.15 25 000
74 200
60,200
24 15 mL J-TB-057
JA-21
Fixed Angle, 40°
(18 place)
rmax = 102 mm
21 21 000 50 400 18 10 mL J-TB-002
JA-20.1
Fixed Angle, 23°
(32 Place)
rmax = 115 mm
(outer row)
rmax = 98 mm
(inner row)
20.1 20 000
51 500
43 900
32 15 mL J-TB-022
JA-20
Fixed Angle, 34°
(8 place)
rmax = 108 mm
20 20 000 48 400 8 50 mL J-TB-003
JA-18.1
Fixed Angle
(24 place)
45° adapter
rmax = 116 mm
25° adapter
rmax = 112 mm
18.1
18 000
17 000
42 100
36 300
24 1.8 mL
24 1.8 mL
J-TB-037
JA-18
Fixed Angle, 23°
(10 place)
rmax = 132 mm
18 18 000 47 900 10 100 mL J-TB-035
JA-17
Fixed Angle, 25°
(14 place)
rmax = 132 mm
17 17 000 39 800 14 50 mL J-TB-017
JLA-16.250
Fixed Angle, 25°
(6 place)
rmax = 134 mm
16.250 16 000 38 400 6 250 mL J-TB-072
Rotor Profile Description
Rotor
Code
Max
RPMa
Max
RCF
(g) Max Capacity
Rotor
Manual
Number