Biosan UVT-B-AR User manual

DNA/RNA UV-cleaner box
UVT-B-AR
Medical–Biological
Research & Technologies
Operating Manual
Certificate
for versions:
V.3AA
V.3AB
V.3AD
V.3AE
V.3A04

Contents
recautions1.
2. General Information
3. Getting Started
4. Operation
5. Specifications
6. Technical Maintenance
7. Warranty, Reclamation Information
8. Declaration of Conformity
Safety P
3

1. Safety precautions
Caution: Do not work in the cabinet or open the front protective screen while
the open UV-lamp is switched ON. Otherwise it can expose the
operator to a dangerous level of UV emission.
Caution! Exposure to UV light is harmful and can cause damage to
unprotected eyes and skin. The UV-cleaner box contains a
powerful source of UV radiation, therefore, before operating the
unit, ensure all personnel working with the UV-cleaner box are
properly protected.
The following symbols mean:
Caution: Make sure you have fully read and understood the present Manual
before using the equipment. Please pay special attention to
sections marked by this symbol.
GENERAL SAFETY
·Use only as specified in the operating manual provided.
·The unit should be saved from shocks or drops.
·After transport or storage allow the unit to dry out (2-3 hrs) before connecting to the
mains.
·Before using any cleaning or decontamination method except those recommended
by the manufacturer, check with the manufacturer that the proposed method will not
damage the equipment.
·Do not make modifications to the design of the unit.
ELECTRICAL SAFETY
·Connect only to the mains with a voltage corresponding to that on the serial number
label.
·Ensure that the switches and plug are easily accessible during use.
·Do not plug the unit into the main outlet without grounding, and do not use extension
lead without grounding.
·Before moving the unit disconnect the power plug from the mains outlet.
·If liquid is split inside the control block, disconnect it from the mains and have it
checked by a competent person.
The operator should wear a closed-front lab coat (fully buttoned),
UV certified safety glasses and gloves, which should overlap the
lab coat cuffs.
4

DURING OPERATION
·Do not operate the unit in environments with aggressive or explosive chemical
mixtures.
·Do not operate the unit if it is faulty or been incorrectly installed.
·For indoor use only.
·Do not use outside laboratory rooms.
·Do not work in the box while the open UV-lamp is switched ON.
BIOLOGICAL SAFETY
·It is the user's responsibility to carry out appropriate decontamination if hazardous
material is spilt on or inside the equipment.
5

2. General Information
Bench top model of DNA/RNA UV-cleaner box UVT-B-AR is specially designed for
laboratories of molecular biology, DNA analysis and genetic engineering.
The front panel of UV-cleaner box with a movable protective screen that has three
locking positions is made of glass coated with a UV-protection film. The working
surface and the rear wall are of stainless steel. The framework, side and top walls are
of steel with chemical resistant powder coating. Built in power socket or an inlet for
power cords (depending on customer choice) is provided for connecting electrical
devices used inside the cabinet.
At the top of the working area of UV-cleaner box there are:
1. Fluorescent lamp (15 W) for working area illumination
2. Open UV lamp (25 W) for working area disinfection
3. UV Recirculator (25 W) for amplicon inactivation inside the UV-cleaner box
A digital timer controls duration of working surface irradiation with UV light in the
range from 0 to 24 hours. In case of opening the protective front screen during UV
irradiation open UV lamp is switched off automatically.
UV recirculator consists of a UV lamp, a fan and dust filters organized in a plastic
case. Operator working in a UV-cleaner box UVT-B-AR with a switched on UV
recirculator is not exposed to UV radiation. It allows continuous treatment of the airflow
with UV light without interrupting working process. Air circulation at a short distance
from the UV lamp combined with reflective surfaces in the air duct results in increased
density of UV rays leading to higher efficiency of disinfection. UV recirculator
generates 100 volumes of UV-cleaner box per one hour of airflow exchange ensuring
maximally aseptic conditions inside the cabinet.
Microbiological studies at the R&D Department of Biosan led by Dr. biol. V.
Bankovsky demonstrated a high level of biosafety and efficacy of UV-cleaner box UVT-
B-AR (maximal level of contamination is 1–3 cfu per 100 liters of air)*.
UV-cleaner box is designed to protect the product, but not the operator, therefore, it
is not recommended to use the UV-cleaner box for working with pathogenic
microorganisms.
Advantages:
• No HEPA filters;
• UV-recirculator;
• Ozone free high density UV decontamination;
• Open UV-lamp is switched off automatically in case of opening the protective
front screen;
* – http://lodz.biosan.lv/efficiencyRUb5.pdf
6

• Long life UV lamps (8000 h);
• Low noise level;
• Low energy consumption;
• Compact “bench top” model for personal labs;
• Optional table with drawer T-4 (on request).
7

3. Getting started
3.1. Unpacking.
Remove packing materials carefully and retain for future shipment or storage of
the unit.
3.2. Complete set.
Unit set includes:
- DNA/RNA UV-cleaner box UVT-B-AR ..................................................1 pce.
- Spare dust filters ..................................................................................4 pcs.
- A spare fuse (inside the fuse holder) .....................................................1 pce.
- Operating manual, Certificate ..............................................................1 copy
4. Operation
Examine the unit carefully for any damage incurred during transit. The warranty
does not cover in-transit damage.
Standard set:
Optional accessories:
- Moving table for UV-cleaner box T-4 .....................................................1 pce.
3.3. Set up
-For moving table T-4: carefully unpack the table and assemble it using as a
giude the enclosed assembling scheme;
- Place UV-cleaner box upon stable surface. Ensure that the unit is placed on a
solid, level surface (not less than 720x550 mm), which is able to support its
weight and the weight of equipment and materials inside, for instance T-4.
Place UV-cleaner box upon surface.
- Position the unit so that there is easy access to the power switches and mains
plug.
Caution! Due to its size and weight the unit requires two people to lift or move it.
8
T-4

4.1. Plug the power cord to the grounded mains outlet. Switch ON the Power switch at
the rear panel (position I).
4.1.1. Check the cable connections strength of the control block and the
intermediate mains cord.
4.2. UV-exposition of the working place.
Caution! Do not work in the box or opened the protective front screen while
the open UV-lamp is switched ON. Otherwise it can expose the
operator to a dangerous level of UV light.
4.2.1. Turn ON switch 2 (Fig.1/2). This automatically turns on the UV-recirculator
with the hidden UV-lamp and activates the timer of open UV-lamp. The UV-
recirculator will operate all the time until switch 2 is turned OFF.
Note! Open UV-lamp operation can be checked with the visible light off
(switch 1 is OFF). Use the indicator in the center of recirculator cover
to check operation of UV lamp inside the recirculator. If indicator is
lights from inside while switch 2 is ON, then the UV lamp is
functioning.
4.2.2. Use the timer keys + and - (Fig.1/4) to set the time (UV-LIGHT TIME
CONTROLLER) of direct UV light exposition of the working place:
( + ) to increase exposition time (step 1 min);
( - ) to decrease exposition time (step 1 min).
If the key is pressed more than 2 sec the increment becomes bigger.
Recommended time of exposition 15–20 min.
4.2.3. Press the key Start (Fig.1/3), the open UV lamp automatically will be turned
on and timer starts counting up the exposition time. Timer indicator shows
actual time: till 1 hour – in minutes and seconds (min:sec), after 1 hour – in
hours and minutes (hh:mm).
After reaching the set time the timer will automatically turn off the open UV-
lamp.
4.2.4. The open UV-lamp can be switched off by pressing Stop/Reset key
9
Fig.1. Control Panel
12
ON
OFF
1 VISIBLE LIGHT
2 UV-RECIRCULATOR / UV-LIGHT
UV-LIGHT TIME CONTROLLER
Start Stop/Reset
SETTING TIME (MIN)
O
I
O
I
124
35
0 15

(Fig.1/5). The set time of exposition is preserved in the memory. The set time
won't be preserved after the complete switching off the UV-cleaner box.
4.2.5. If the set time of direct UV light exposition is 0:00, pressing the Start key unit
operates continuously during 24 hrs or until the Stop/Reset key is pressed.
UV-cleaner box is ready for use.
4.3. Work in the Box.
Note: Opening protective screen the open UV-light switches off, but timer
continue counting up exposition time.
4.3.1. Turn ON switch 1 (Fig.1/1) for lighting of the working place (this turns ON the
Luminescent (Visible light) lamp).
4.3.2. Open the front protective screen up for work in the box.
4.3.3. After the task is done close the front protective screen.
4.3.4. At the end of operation turn OFF switch 2 and switch 1.
4.4. Turn OFF the unit with the power switch (position O) and disconnect the power
plug from the mains outlet.
5. Specifications
10

The unit is designed for operation in cold rooms, incubators and closed laboratory
rooms at ambient temperature from +4°C to +40°C and maximum relative humidity
80% for temperatures up to 31°C decreasing linearly to 50% relative humidity at 40°C.
·Open UV-lamp.............................................TUV 25 W G13 UV-C Special Philips
·Radiation type ...............................................................UV (254 nm), ozone free
·UV-lamp life time ....................................................................................8000 hrs
·UV-recirculator ............................................TUV 25 W G13 UV-C Special Philips
·Digital time setting of direct UV-exposure .........................................1 min – 24 h
·Luminescent (Visible light) lamp.........................................15 W/830 G13 Philips
·
·
·
·
·
·Thickness of upper front panel ......................................................................8 mm
·Thickness of protective front screen .............................................................4 mm
·Working place ....................................................................................650x475 mm
·Overall size ................................................................................ mm
·
·........................................................................................
·Weight*........................................................................................................31.2 kg
* Accurate within ±10%
Glass type ...........................................................................Euroglass, (Germany)
Side walls material type.....................................................................painted steel
Optical transmission ......................................................................................95 %
UV protection ................................................................................................96 %
Protection film type ..........................................................................4 MIL CLEAR
690x585x555
Power outlet inside the unit (exclude V.3AA)................................................1 piece
67 W
** Note! Consumed power of device connected via internal power socket should
not exceed 1000 W for 230V.
6. Technical Maintenance
Power consumption
Biosan is committed to a continuous programme of improvement, specifications may
be changed without notice.
11
DescriptionOptional accessories Catalogue number
moving table with a drawer and wheel
locks, dimensions 800x600x745 mm
T-4 BS-040101-BK
Operating voltage Power outlet
100–240 V, 50/60 Hz —
100–240 V, 50/60 Hz US**
100–240 V, 50/60 Hz UK**
Version number
V.3AA
V.3AE
V.3AB
100–240 V, 50/60 Hz Euro**V.3AD
Plug
Euro
US
UK
Euro
100–240 V, 50/60 Hz AU**V.3A04 Euro

6.1. If service is required, disconnect the unit from the mains and contact your local
Biosan representative or Biosan.
6.2. It is allowed to perform the maintenance and all types of repair operations only to
the persons who have completed special training.
6.3. Replacement of fuses
Remove the power plug from the rear of the
unit.
(3,15 A for 100–240 V,
50/60 Hz).
6.4. Replacement of UV-lamps
Average life time of UV lamps supplied is 8000 hrs. Replacement is necessary
after lamp stops functioning or at the end of manufacturer specified life time.
It is allowed to perform lamps replacement only to the persons who have
completed special training.
Open UV-lamp operation can be checked with the visible light off (switch 1 is OFF).
Use the indicator in the center of recirculator cover to check operation of UV lamp
inside the recirculator. If indicator lights from inside while switch 2 is ON, then the
UV lamp is functioning.
6.5. Cleaning and maintenance
For decontamination it is recommended to use:
·70% Ethanol;
·sodium hypochlorite solution;
TM
·DNA/RNA removing solution (e.g. DNA-Exitus Plus ,
TM
RNase-Exitus Plus ).
After washing the inside parts of the box it is necessary to rub them dry.
Caution! Do not allow moisture to enter the control box.
6.6. Replacement of Dust Filters
The dust filters on either end of the UV-recirculator with the hidden UV-lamp should
be checked monthly and cleaned or replaced when they become clogged. To
check, replace or clean the filters, simply unclip the covers, if it is necessary fit a
new or rinse in water, dry and set up existing filters. Clip covers back in place.
7. Warranty. Reclamation information
Disconnect the unit from the mains outlet.
Pull out the fuse drawer by applying
leverage in recess (Fig.2/A). Remove the fuse
from the holder. Check and replace with the
correct fuse if necessary
12
Fig.2 Fuse
replacement
A

13
7.1. The Manufacturer warrants the compliance of unit with the requirements of
Specifications, provided the Customer follows the operation, storage and
transportation requirements.
7.2. The warranted service life of unit from date of delivery to the Customer is 24
months (exclude lamps and dust filters). Turn to your local distributor to check
availability of extended warranty.
7.3. If any manufacturing defects are discovered by the Customer an unsatisfactory
equipment claim shall be compiled, certified and sent to the local distributor
address. Please visit www.biosan.lv, Technical support section to obtain the claim
form.
7.4. The following information will be required in the event that warranty or post-
warranty service comes necessary. Complete the table below and retain for your
records.
DNA/RNA UV-cleaner box UVT-B-AR
Model
Serial number
Date of sale

8. Declaration of Conformity
14

Version 3.03 – October 2013
Biosan SIA
Ratsupites 7, build. 2, Riga, LV-1067, Latvia
Phone: [phone]
Fax: [phone]
http://www.biosan.lv