Biosan Inteliwasher 3D-IW8 User manual

Page 2
Contents
1. About this edition of user instructions.......................................................................2
2. Safety precautions ...................................................................................................3
3. General information..................................................................................................5
4. Getting started..........................................................................................................6
5. Operation ...............................................................................................................10
6. Operation closedown .............................................................................................14
7. Program error messages........................................................................................15
8. Specifications.........................................................................................................16
9. Ordering information ..............................................................................................17
10. Care and maintenance...........................................................................................18
11. Warranty.................................................................................................................21
12. Glossary.................................................................................................................22
13. EU declaration of conformity..................................................................................23
1. About this edition of user instructions
The current edition of the user instructions applies to the following models and ver-
sions of microplate washers:
Inteliwasher 3D-IW8 .........................................................................version V.3AW

Page 3
2. Safety precautions
Attention! Please make sure you have fully read and understood the Manual
before using the equipment and pay special attention to sections
marked by this symbol.
GENERAL SAFETY PRECAUTIONS
Use only as specified in the Operating Manual provided.
Protect the unit from shocks and falling.
After transportation or storage, keep the unit at room temperature for 2-3 hours before
connecting to the mains.
Before diagnostics, study carefully the possibilities and operation procedures of the
unit in order to obtain reproducible and credible results. Check any unclear issues
with the supplier.
It is not recommended to leave the operating unit unattended for a long period.
Change the bottles or connecting tubes only when the unit is switched off.
In case of doubt about the quality and uniformity of the completed washing program,
disregard the results obtained and repeat the washing procedure.
Accuracy of the obtained testing results depends on the quality of the microplate rins-
ing procedure. To assure correctness of the rinsing procedure, regularly exercise vis-
ual control over reproducibility of liquid volume dosing.
Before using any cleaning or decontamination methods except those recommended
by the manufacturer, check with the manufacturer that the proposed method will not
damage the equipment.
Do not make modifications to the design of the unit.
ELECTRICAL SAFETY
Connect only to an external power supply with voltage corresponding to that on the
serial number label. Use only the external power supply provided with this product.
Do not plug the power supply into an ungrounded power socket, and do not use an
ungrounded extension lead.
Ensure that the switch and external power supply connector are easily accessible
during use.
Disconnect the unit from the mains before relocating it.
If liquid penetrates into the unit, disconnect it from the mains and do not use it until it
is checked by a repair and maintenance technician.
Do not operate the unit in premises where condensation can form. Operating condi-
tions of the unit are defined in the Specifications section.

Page 4
DURING OPERATION
Do not operate the unit in environments with aggressive or explosive chemical mix-
tures. Please contact manufacturer for possible operation of the unit in specific at-
mospheres.
Do not operate the unit if it is faulty.
Always have the cover protecting against aerosol spread (hereinafter protective
cover) installed during operation.
Do not operate the unit if any of the needles is clogged or malfunctions.
Never touch the needles or place fingers or other parts under the needles during
operation. Manifold needles are sharp and can cause injury.
Always switch off the unit before changing the manifold.
If liquid spills on the guiding rail, stop the operation, clean and wipe the surface dry.
Do not allow the waste bottle to overflow the maximum level during operation (there
is a mark on the side surface of the bottle). Take necessary precautions utilizing
waste liquid in accordance with general laboratory standards.
AFTER OPERATION
Carry out the cycle of system washing (on the unit front panel, use the button System
rinsing) with distilled water and do not dry. This will keep the system in permanent
operation condition.
Clean the guiding rail under the platform and wipe it dry to prevent oxidation layer
formation and to prolong working life of the unit.
BIOLOGICAL SAFETY
It is the user’s responsibility to carry out appropriate decontamination if hazardous
material is spilt on or penetrates into the equipment.
In diagnostic researches, potentially dangerous biological materials can be used.
When working with such materials, always use protective clothing and eye protection.
Always have the protective cover installed during operation.

Page 5
3. General information
Inteliwasher 3D-IW8 series microplate washer is designed for washing various types
of standard microplates, microstrips and microarrays on FastFRAME (rectangular well shape
frame). Washer is suitable for washing wells with different bottom shapes, flat, U-shape and
V-shape. The unit is fully programmable, ensuring multi-step solution ripening, aspiration
and soaking cycle during a particular period of time, as well as combinations of aspiration,
liquid dispensing and soaking.
The unit has 50 programs divided into 5 following aspiration categories (Scheme 1):
Type 1 (programs 1.0–1.9) IPF96 U/V is intended for U-shape and V-shape immuno-
plates, 1 point aspiration.
Type 2 (2.0–2.9) IPF96 FLAT-2 is intended for flat-bottom shape immunoplates, 2
point aspiration.
Type 3 (3.0–3.9) IPF96 FLAT-C is intended for rectangular shape immunoplates, full-
circle aspiration direction.
Type 4 (4.0–4.9) FastFRAME-2 is intended for multi-slide
1
plate with rectangular
wells.
Type 5 (5.0–5.9) FastFRAME-C is intended for multi-slide1plate with rectangular
wells.
Note. Optional 4-channel washing solution weight logger, 4CHW Logger al-
lows automatic buffer bottles and waste bottle volume control.
The unit is supplied with 8-channel washing head for dispensing/aspiration, 3 bottles for
washing and rinsing solutions, a large waste bottle and small aerosol catching bottle with hydro-
phobic filter that eliminates risk of contamination with bacteria, viruses and infected particle from
wasted liquid to an atmosphere. Hydrophobic filter bacterial efficiencies is very high, up to
99.999% particles bigger than 0.027 μm (which is smaller than Hepatitis A, B and C).
The unit provides:
washing mode;
rinsing mode;
mixing mode;
single point, two point, circular (circle or rectangular path);
possibility of additional solution mixing during time gap between two work cycles;
possibility to use microtest plates by different manufacturers, ensured by automated
plate set up (adjust-ing to different depths of plate wells);
round-bottom plate and strip washing mode;
possibility of user-defined programs with adjustable parameters.
1
The FastFRAME (Schleicher&Shuell) multi-slide plate or analog plate of another manufac-
turer, that is compatible with standard 1 x 3 inch (25 x 76 mm) glass slides.

Page 6
4. Getting started
4.1. Unpacking. Remove packing materials carefully and retain them for future shipment
or storage of the unit. Examine the unit carefully for any damage incurred during
transit. The warranty does notcover in-transit damage.Warranty covers only the units
transported in the original package.
4.2. Complete set. The set includes:
#
Name
Quantity
1
3D-IW8 Inteliwasher
1 piece
2
Platform for plates
1 piece
3
Manifold
1 piece
4
Protecting cover
1 piece
5
External power supply
1 piece
6
Tubes (outside/inside diam./length 6/3/600 mm)
5 pieces
7
Tube for manifold (outside/inside diam./length 3.2/1.6/400 mm)
1 piece
8
Tube for manifold (outside/inside diam./length 5/3/440 mm)
1 piece
9
Tube for hydrophobic filter (outside/inside diam. 9/6 mm)
1 piece
10
Half-litre bottle with connectors for aerosol collection
1 piece
11
1-litre bottles with sieve filters and connectors for reagents
3 pieces
12
2-litre bottle with connector for collecting of waste liquid
1 piece
13
Hydrophobic filters for half-litre bottle
2 pieces
14
Manifold cleaning set
1 piece
15
Syringe for liquid flushing in hoses
1 piece
16
Power cord
1 piece
17
Operating instructions,declaration of conformity
1 copy
18
4-channel washing solution weight logger, 4CHW Logger
on request
Figure 1. Complete set

Page 7
Figure 2. Unit side and back overview. Setting up.
4.3. Storage and transportation.
- During long-term storage, keep the unit and all its accessories in original packaging
in a dry dust-free place.
- Use the original packaging for transportation whether transported by air, water or
land.
Attention! We do not accept warranty claims related to damages caused by im-
proper packaging.
- To pack the unit properly, see 10.8.
4.4. Setup.
4.4.1. Place the unit on a strong horizontal surface, which can safely support weight of the
unit. To provide optimal ventilation, ensure 100 mm clearance on each side. Opera-
tion conditions are described in the Specification section.
4.4.2. Remove the protecting cover.
4.4.3. Unpack the plate platform.
4.4.4. Place the plate platform on the railing (fig. 2/1) so that the plate holder (flat spring)
faces the rear side of the unit (fig. 2/2). Magnet on the other side of the rail locks the
platform into start position.
Note: If the plate platform is installed improperly, the magnet does not hold
it in place and the platform will be able to move freely. Move the plat-
form along the railing until the magnets lock. Increase of resistance
to movement indicates that the magnets are locked.
4.4.5. Unpack the manifold.

Page 8
4.4.6. Install the manifold in the slots of the holder arm (fig. 2/3). Magnets in the manifold
and the holder arm connect and align the manifold.
4.4.7. Connect the manifold inlet connector supplying the liquid and the blue-coloured
Washing Head Dispense connector at the rear panel of the unit (fig. 3/2) with a tube
(fig. 1/7).
4.4.8. Connect the manifold outlet connector and the yellow-coloured Washing Head As-
pirate connector at the rear panel of the unit (fig. 3/4) with a tube (fig. 1/8).
Note: Use the provided labels for marking bottles with washing solutions
and bottles for collecting waste liquid and aerosol.
4.4.9. Connect the #4 green-coloured Waste bottle outlet on the rear side of the unit to the
connector of 2-litre bottle #4 for waste liquid collecting (fig. 3/1).
4.4.10.Place the tube for the hydrophobic filter (fig. 1/9) on the connector of the half-litre
bottle #5, then place the hydrophobic filter on the tube (fig. 1/13). Check that the “IN”
marking on the filter is facing the bottle #5 (fig. 11).
4.4.11.Connect the Waste bottle #4 to the half-litre bottle #5 (fig. 3/10).
4.4.12.On the rear side of the unit:
4.4.13.Connect the bottle #1 to the inlet connector (#1 –red, fig. 3/3) with a tube (fig. 1/6).
4.4.14.Connect the bottle #2 to the inlet connector (#2 –black, fig. 3/5) with a tube (fig. 1/6).
4.4.15.Connect the bottle #3 to the inlet connector (#3 –white, fig. 3/6) with a tube (fig. 1/6).
4.4.16.Insert the manifold tube (fig. 3/2) into the valve opening (fig. 2/4). Press the valve in
the direction of the arrow and hold the valve while stretching the tube and pulling it
through the opening. Release the valve.
Note: Make sure that all connections are secured tightly.
4.4.17.Install the protective cover. Ensure that all tubes are covered, but are not squeezed.
4.4.18.Place bottles near the unit.
4.4.19.If 4-channel weight logger is included in the kit, remove pads from underside of scale
cups. Place bottles on the 4-channel weight logger according to the numbering. Con-
nect the RS-232 with the cable to the plug connector on the rear side of the unit (fig.
3/7).

Page 9
Figure 3. Assembly diagram
4.5. Switching on.
Warning! Before connecting the unit to the mains outlet, check that the power
switch (fig. 3/9) is in position O(off).
4.5.1. Connect the external power supply to the power socket (fig. 3/8) on the rear panel of
the unit.
4.5.2. Connect the power cord to the external power supply (fig. 1/16)
4.5.3. Connect the external power supply to the grounded power socket.
4.5.4. Switch on the unit (position I) using the power switch on the rear panel.
4.5.5. Whenswitched on, the unit performs full initialisation cycle and displays the message”
Power on reset”
Note: Full initialisation cycle takes no longer than 5 seconds.

Page 10
5. Operation
5.1. Before starting plate washing:
- Fill the rinsing liquid bottle (#3) with distilled water or appropriate cleansing solution.
- Fill the necessary bottles (#1 and/or #2) with washing solutions.
5.2. Initial parameters of all programs are given in Table 1. All values are adjustable. User
programs can be created by changing the template of the original program. All pro-
grams are divided into 5 categories with 10 programs each, see figure 4. Each cate-
gory corresponds to a different type of microplate:
Category IPF96 U/V (1) is intended for U-shape and V shape well immunoplates.
Categories IPF96 FLAT-2 (2) and IPF96 FLAT-C (3) are intended for flat-bottom well
immunoplates.
Categories FastFRAME-2 (4) and FastFRAME-C (5) are intended for Watman
Schleicher & Shuell FastFRAME plates with rectangular wells.
Program #
01
02
03
04
05
06
07
08
09
10
Dispense
NO
YES
YES
YES
YES
YES
YES
YES
YES
YES
Aspirate
YES
YES
YES
YES
YES
YES
YES
YES
YES
YES
Shake
NO
NO
NO
NO
NO
NO
NO
NO
NO
NO
Dispense rate
03
03
03
03
03
03
03
03
03
03
Aspirate rate
03
03
03
03
03
03
03
03
03
03
Soak time, s
-
30
30
30
30
30
30
30
30
30
Shake time, s
-
-
-
-
-
-
-
-
-
-
Dispensed volume, μl
-
350
350
350
350
350
350
350
350
350
Aspiration time, s
1
1
1
1
1
1
1
1
1
1
Final aspirate, s
-
2
2
2
2
2
2
2
2
2
First aspirate
-
NO
YES
YES
NO
YES
YES
YES
YES
NO
Wash by rows
-
NO
YES
YES
NO
YES
YES
YES
YES
NO
On two channels
-
NO
NO
YES
NO
YES
YES
NO
NO
NO
Num.of 1 wash cycles
01
01
05
05
01
03
05
07
07
07
1 channel
-
-
-
02
-
02
02
-
-
-
Num.of 2 wash cycles
-
-
-
01
-
01
01
-
-
-
2 channel
-
-
-
01
-
02
01
-
-
-
Table 1. Initial parameters of all user programs.

Page 11
5.3. All possible program parameters and their meaning are shown in Table 2.
Table 2. Program parameters and their definitions
Parameter
Value or range
Description
Dispense
yes/no
Perform fill
Aspirate
yes/no
Perform aspiration1
Shake
yes/no
Plate shaking on the platform during the cycle
Dispense rate
01..03
Speed of liquid filling (100; 200; 300 μl/s)
Aspirate rate
01..03
Speed of liquid aspiration
Soak limit, s
0..300 (step 10 s)
Time between fill and aspiration
Shake limit, s
05..150 (step 5 s)
Shaking time
Dispensed volume
25…1600
(step 25)
Volume of dispensed liquid
Aspirate time, ms
200..3000 ms (step 200 ms)
Time of aspiration from well in cycle
Final aspirate, s
200..3000 ms (step 200 ms)
Time of last aspiration in the cycle
First aspirate
yes/no
Aspiration is the first action in a cycle
Wash by rows
yes/no
Each row is washed once per cycle
On two channels
yes/no
Use 2 washing solutions
Num.of wash cycles
1..15
Number of washes with first solution
1 channel
1..3
Number of bottle to use for the main washing
Second chan. cycles
01..15
Number of washes with second solution
2 channel
1..3
Number of bottle to use for the second washing
5.4. Install the plate on the platform.
Attention! When installing a plate the first time or when a different type of plate
is installed perform plate setup to adjust the manifold lowering depth
(see paragraph 4.20).
Note: When working with the FastFRAME plates, remove the plate holder
(fig. 2/2). Install it back when working with immunoplates.
5.5. Use the Enter (fig. 4/1) key to select category, depending on the used plate type. Use
the +and -keys (fig. 4/3) to select required washing program from 1 to 10. Program
category and number are displayed in the top right corner of the display (fig. 5/1)
5.6. Press the Program Parameters key (fig. 4/4) to view the parameters of program. In
the program, 1st parameter is activated (Dispense...yes/no, fig. 4/5).
5.7. Use the +and -keys to select necessary value and press the Enter key to save
changes to the active parameter and proceed to the next. This way you will be able
to review and/or change all parameters.
5.8. Press the Rowskey (fig. 4/6) to return to the previous parameter in the program.
5.9. Press the Program Parameters key to exit parameter view and change mode and
save the program.
5.10. Press the Esc key (fig. 4/7) to exit parameter view and change mode without saving.
5.11. Press the Run/Stop key (fig. 4/2) to start the program.
5.12. If it is necessary to wash less than 12 rows on a microplate, press the Rows key (fig.
4/6) before starting. Following message appears on the display, figure 6.
1
During aspiration, the waste fluid bottle is detected automatically –bottle #4. The number is not spec-
ified on the display

Page 12
Figure 5. Program selection
Figure 4. Control panel
Figure 6. Setting number of rows to rinse
Figure 7. Display during operation
5.13. Select necessary number of rows to rinse using +and -keys.
5.14. Press the Run/Stop key to save changes and start the unit operation.
5.15. If operation requires washing from two channels (parameter “On two channels” set to
YES), confirmation of the correct bottle number for second wash appears on the dis-
play before starting the program (fig. 9). Select necessary bottle number using +and
-keys and press the Run/Stop key.
5.16. During operation, display shows program number, currently used bottle number and
the number of cycles left (fig. 7). If the current action has time tracking (soaking or
shaking), then the elapsed time is displayed.
5.17. If 4-channel washing solution weight logger is connected to the unit, then during op-
eration, display shows program number, currently used bottle number, the number of
cycles left and the remaining volume percentage of liquid in the bottles (fig. 8). For
the waste bottle, filled liquid amount is displayed accordingly based on 2-litre bottle
volume percentage.
5.18. To stop the program during operation, press the Run/Stop key. The message “Can-
celled by operator” appears on the lower line of the display. Press the Run/Stop key
to restart the washing.
Note: If operation requires washing from one channel (parameter “On two
channels” set to NO), washing parameters for the second channel are
not displayed. Respectively, if two channels are set (parameter “On
two channels” set to YES), washing parameters for the second chan-
nel are displayed and can be adjusted as needed (Table 3).

Page 13
Figure 8. Display during operation with con-
nected 4-CHW Logger
Figure 9. Choosing the bottle number for rinse
on the second channel
5.19. When the cycle is completed, unit produces an informative sound signal. Proceed to
section Operation closedown of these instructions.
Attention! Always perform the rinsing cycle using distilled water after finishing
the operation at the end of working day. This will help to keep the unit
in working order and prevent head channel clogging.
5.20. Plate setup. When installing a plate for the first time or when a plate of different type
is used, setup the manifold needles lowering depth. Press the Enter key in the start
mode, the following message appears on the display (fig. 10). To calibrate, press
Run/Stop key. The manifold measures the depth of the plate well by touching the
plate surface first and then the bottom of the well. The unit saves the difference of the
measured values.
Table 3. All parameters of programs with two
channels
Dispense
YES
Aspirate
YES
Shake
YES
Dispense rate
03
Aspirate rate
03
Soak limit, s
00
Shake limit, s
30
Figure 10. Manifold lowering depth
identification
Dispensed volume
300
Aspirate time, ms
1000
Final aspirate, ms
2000
First aspirate
YES
Wash by rows
YES
On two channels
YES
Num.of wash cycles
03
1 channel
01
2 chan. cycles
01
2 channel
02

Page 14
6. Operation closedown
6.1. When the cycle is completed, a sound signal will inform that the unit stopped the
operation.
Attention! Always perform the rinsing cycle using distilled water after finishing
the operation at the end of working day. This will help to keep the unit
in working order and prevent manifold channel clogging.
6.2. Press Prime rinse (fig. 11/1), display shows “PRIME RINSE, the channel 01”. Select
the correct channel with using +and -(fig. 11/2). Press Run/Stop (fig. 11/3) key and
the system will perform rinsing cycle. Repeat the procedure twice, if necessary.
6.3. Disconnect the tube from selected bottle, and press Prime rinse key again to dry the
tube with air.
6.4. After finishing the operation, remove the tube from the valve opening to prevent de-
formation (wall gluing) of the dosing valve tube. To remove the tube, press the valve
on the side (fig. 2/4, 2/) and stretch the tube a little when pulling it through an open-
ing.
6.5. Switch the unit off using mains switch on the rear panel (position O, off).
6.6. Unplug the external power supply from the mains.
Figure 11. System rinse

Page 15
7. Program error messages
7.1. Table 4 shows the program cycle error messages, their description and possible so-
lutions that can be made by user.
Table 4. Program error messages
Displayed message
Description
CANCELED BY OPERATOR
Program execution is stopped by operator.
Press Run/Stop key
BOTTLE 4 OVERFLOWED
Overflow of waste collection bottle. Empty the bottle
BOTTLE ## EMPTY
Buffer bottle needs to be filled. Fill the bottle
PLATE ERROR
Plate calibration is required. See 6.20
7.2. If the unit stops the operation and an unmentioned message appears, copy the error
text, unpower the unit and contact your service company.

Page 16
8. Specifications
The unitisdesignedforoperationincoldrooms andclosedlaboratoryroomsatambient
temperature from +4°C to +40°C in a non-condensing atmosphere and maximum relative hu-
midity 80% for temperatures up to 31°C decreasing linearly to 50% relative humidity at 40°C.
Biosan is committed to a continuous programme of improvement and reserves the
right to alter design and specifications of the equipment without additional notice.Dispense
system of liquid dosage for each channel separately;
Minimum dispense volume ...........................................................................................25 µl
Maximum dispense volume ......................................................................................1600 µl
Dispense increment ......................................................................................................25 µl
Irregular liquid dosage at 300 µl ...........................................................max. ±2.5% or 7.5 µl
Allowed residual liquid volume in plate well ...........................................................max. 2 µl
Number of wells washed simultaneously ............................................................................8
Number of washing cycles........................................................................................... 1 - 15
Aspiration time ............................................................................................................1 - 3 s
Aspiration/dispensing speed .....................................................................................3 levels
Washing buffers .................................................................................................3 maximum
Maximum number of washing liquids in program ................................................................2
Dispense system...........................................................................................sphincter valve
Soaking time ...............................................................................0 –300 s (increment 10 s)
Shaking time..................................................................................0 –150 s (increment 5 s)
Number of washed rows .............................................................................................1 –12
Time of plate single wash (350 µl) ........................................................................max. 45 s
Number of programs .........................................................................................................50
Plate platform and manifold movement ...............................................................automated
Indication of operation modes .............................................................................8-line LCD
Dimensions .............................................................................................. 375х345х180 mm
Weight
1
.........................................................................................................................11 kg
Input current/power consumption ............................................................12 V, 1.8 A / 22 W
External power supply.................................input AC 100–240 V 50/60 Hz, output DC 12 V
1
Accurate within ±10%.

Page 17
9. Ordering information
9.1. Models and versions available:
Model
Version
Catalogue number
3D-IW8
V.3AW
BS-010212-AAA
9.2. To inquire about or order the optional accessories or the replacement parts, contact
Biosan or your local Biosan representative.
9.2.1. Optional accessories
Accessory
Description
Catalogue number
4CHW Logger
4-channel washing solution weight logger, max.
loading per scale cup 2 kg, dimensions
267x252x97 mm, weight13 kg
BS-060102-AAI
9.3. Replacement parts
Replacement
Description
Catalogue number
Bottle #1
Assembled (with weight, tube, filter)
BS-060102-S26
Bottle #2
Assembled (with weight, tube, filter)
BS-060102-S27
Bottle #3
Assembled (with weight, tube, filter)
BS-060102-S28
Bottle #4
Assembled with tube
BS-060102-S29
Bottle #5
Assembled (with filter, tube)
BS-060102-S43
Hydrophobic filter
For bottle #5
BS-060102-S44
Filter complete set
Filter, weight, tube
BS-060102-S01
Silicone tube set
6 pcs.
BS-060102-S39
1
Accurate within ±10%.

Page 18
10. Care and maintenance
10.1. If the unit requires maintenance, disconnect the unit from the mains and contact your
local Biosan representative or Biosan service department.
10.2. All maintenance and repair operations must be performed only by qualified and spe-
cially trained personnel.
10.3. Do not use non-conforming parts for repair. The manufacturer provides all necessary
services and spare parts. To order necessary services and parts, please contact your
supplier.
10.4. Maintenance checks. The following checks can be done by user.
10.4.1.Daily maintenance.
- Clean the surface of the device from liquid droplets and contamination.
- Clean and wipe dry the surface around the guiding rail (fig. 2/1).
- Perform the rinsing cycle twice using distilled water after finishing the operation (see
section 5).
10.4.2.Monthly.
- With 75% ethanol solution, clean mounting surface of the plate platform and plate
holder spring.
10.5. Ethanol (75%) or other cleaning agents recommended for cleaning of laboratory
equipment can be used for cleaning and disinfection of the unit.
10.6. Filter replacement (fig. 11). It is recommended to change the hydrophobic filter once
in six months; in particular cases, e.g. at intensive operation, once in three months
(to order the filter, you have to know the product identification number, see the table
in Specifications chapter). Disconnect the filter from outlet tube, remove the filter,
insert a new filter into the socket and tighten it.
Attention! Maintain the mounting of the filter according to figure 11. The “IN”
marking should be located on the side of the inlet tube (facing the
bottle).
Figure 11. Filter mount

Page 19
10.7. Troubleshooting
Table 5. Troubleshooting
Symptom
Possible cause
Action required
Manifold dosing error
Volume of washing
solution does not
correspond to the set
volume, dosing une-
venness is observed
along the plate wells
or the washing solu-
tion is not dispensed
at all.
Poor contact between the
bottle hose connector and
the device
Ensure proper connection of the bottle with the de-
vice.
The hose is overbent
Check if the hose is overbent and straighten it if
necessary.
Obstruction of sieve filters
Check if the sieve filters in bottles No. 1, 2 and 3
are clogged.
Obstruction of dispensing
channel of the manifold or
a needle
Check if the dispensing channel of the manifold or
needles is not obstructed. If yes, perform cleaning
(see “Obstruction of dispensing channel or a nee-
dle in the manifold” in this Table).
Absence of liquid in bottles
Ensure presence of liquid in the bottles No. 1, 2
and 3; fill up liquid in a bottle if needed. (Attention:
with 4CHW Logger, information on liquid levels in
bottles is shown on the display).
Obstruction of dis-
pensing channel or a
needle in the mani-
fold
During operation needles
of the manifold can be ob-
structed.
Determine the obstructed needle by checking the
underfilling of a well.
Warning! Be careful working with the manifold,
prevent pricks and injuries caused by needles, they
may contain dangerous infections!
Take off the manifold from the device, put it down
with needles upward. Clean the aspiration channel
needles with the wiping rod Ø 0.80 mm, and clean
needles of the dispensing channel with the wiping
rod Ø 0.45 mm, inserting the wiping rod against
stop and removing it.
After cleaning, install the manifold on the device
and do the rinsing procedure to ensure proper op-
eration of the needle.
In case of repeated obstruction, execute require-
ments of pp. 1 and 2, unscrew the manifold’s plugs,
disconnect the dispensing and aspirating tubes,
clean the dispensing or aspiration channel with the
wiping rod, rinse the manifold with the 75% solution
of ethanol, replace the plugs, install the manifold on
the device and ensure the proper operation of nee-
dles.
Washing solution is
not dispensed into a
plate
Deformation (wall gluing)
of dosing valve tube due to
the long-term storage or
standstill of the device
Remove the tube from the valve.
In the case of deformation, open with fingers the
closed part of the dosing valve.
Insert the tube into the valve opening to position
shown in fig. 2/4.
Note: In order to release the tube, press the valve
on the side and slightly stretch the tube pulling it
through the opening.
Wear or damage of
the valve tubes and
the pump
The connecting tubes are
worn out or damaged dur-
ing exploitation.
Turn off the device and have it checked by a spe-
cialist. Only service engineers and trained special-
ists are allowed to replace the tubes.

Page 20
Table 5. Troubleshooting (cont.)
Symptom
Possible cause
Action required
Volume of residual
liquid in a well ex-
ceeds the admissible
value (2 μl)
Depth of the manifold im-
mersion is not determined.
Perform well depth measurement in the automatic
mode.
Obstruction of the manifold
aspiration channel or a
needle.
Check if the aspiration channel and needles are not
obstructed. If yes, do their cleaning (see “Obstruc-
tion of dispensing channel or a needle in the mani-
fold” in this Table).
If it does not help, turn off the device and have it
checked by a specialist.
On starting the oper-
ation, the device
does not fetch liquid
from the active bot-
tle.
Insufficient liquid quantity
in the pipe.
To solve this problem, complete the following pro-
cedure:
- Stop the program.
- Prepare a syringe with 5 ml of distilled water.
- Unscrew the hose from the active bottle.
- Press Prime Rinse key.
- Using + and - keys, select number of the active
bottle and press the Start key.
- At the moment when the pump begins operation,
gradually fill the hose with distilled water from the
syringe.
- Tighten the hose on the bottle.
If the pump does not restore performance, contact
the service engineer
10.8. Storage and transportation.
10.8.1.Remove the manifold, tubes and platform for plates.
10.8.2.Put on packaging blocks onto sides of the unit and place it into the original carton
box.
10.8.3.Place the manifold into protecting case; place syringe, manifold cleaning set and the
platform into PE bag. Place the bag and the case with the manifold in the corners of
the box between the packaging blocks and the side panels of the carton box; place
the 2-litre bottle and the half-litre bottle in free space between the packaging blocks
and the side panels of the carton box.
10.8.4.Insert the top section into the carton box by bending the section downwards along
the perimeter and inserting thebent parts between the walls of the original carton box
and packaging blocks, so that the device and the remaining content of the box are
fully covered.
10.8.5.Put the soft foam sheet (grey) with the smooth side down on the resulting horizontal
carton surface.
10.8.6.Put the remaining three bottles and other accessories on the foam sheet.
10.8.7.If 4-channel weight logger is included in the kit, insert pads under scale cups, then
place the logger into top section of packaging.
10.8.8.Cover everything with another sheet of soft foam material with the smooth side up.
10.8.9.Close the box and seal with packing tape.