Biosan Automatic Luminescence Analyser ALA1/4 User manual

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Contents
1. Safety Precautions .......................................................................................4
2. General Information......................................................................................5
3. Getting started..............................................................................................6
4. Operation......................................................................................................7
5. Specifications ...............................................................................................8
6. Maintenance.................................................................................................9
7. Warranty and Claims..................................................................................10

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1. Safety Precautions
The following symbol means:
Caution: Make sure you have fully read and understood the present
Manual before using the equipment. Please pay special at-
tention to sections marked by this symbol.
GENERAL SAFETY
Use only as specified in the operating manual provided.
Save the unit from shocks and falling.
After transportation or storage, keep the unit under room temperature for 2-
3 hrs. before connecting it to the electric circuit.
Before using any cleaning or decontamination methods except those rec-
ommended by the manufacturer, check with the manufacturer that the pro-
posed method will not damage the equipment.
Do not make modifications in design of the unit.
ELECTRICAL SAFETY
Connect only to electric circuit with voltage corresponding to that on the
serial number label.
Ensure that the switch and plug are easily accessible during use.
Do not plug the unit into an ungrounded power socket, and do not use an
ungrounded extension lead.
Disconnect the unit from electric circuit before moving. Switch the unit off
and disconnect the power cord plug from power socket to disconnect the unit
from electric circuit.
It is the user’s responsibility to carry out appropriate decontamination if
hazardous material is spilt on or penetrates into the equipment. If liquid pen-
etrates into the unit, disconnect it from electric circuit and have it checked by
a repair and maintenance technician.
Do not operate the unit in premises where condensation can form. Operating
conditions of the unit are defined in the Specifications section.
DURING OPERATION
Do not operate the unit in environments with aggressive or explosive chemi-
cal mixtures. Please contact manufacturer for possible operation of the unit
in specific atmospheres.
Do not operate the unit if it is faulty or was installed incorrectly.
Do not use outside laboratory rooms.
Do not operate the unit while the lid is open.
BIOLOGICAL SAFETY
It is the user’s responsibility to carry out appropriate decontamination if
hazardous material is spilt on or penetrates into the equipment.

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2. General Information
Multi channel rotor-type Fluorimeter developed for the end-point PCR prod-
uct detection. It is a 4 channel high speed detector of Fluorescence/quenching
ration of DNA probe conjugated with a fluorescence/quenching molecules. Level of
fluorescence depends on a quantity of a positive amplification after thermocycling of
DNA probes. The instruments substitute electrophoresis and gel-documentation
steps of amplicon detection. The close tubes method ensures a biosafety conditions
in the DNA diagnostic Labs.
The product consists of the flowing main parts:
A rotor with tubes sockets fixed on the motor axis
Optical module
Microprocessor module
The operation is based on detection of fluorescent radiation of a certain
wavelength that is created in the reaction material by a certain wavelength light
from the excitation radiation source. The detection is performed in each tube one
after another which are delivered to the optical block by the motor. The micropro-
cessor module controls the operation of the analyser main parts and data exchange
with the controlling PC.

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3. Getting started
3.1. Unpacking
Remove packing materials carefully and retain them for future shipment or
storage of the unit.
Examine the unit carefully for any damage incurred during transit. The
warranty does not cover in-transit damage.
Warranty covers only the units transported in the original package.
3.2. Complete set. Package contents:
Standard set
- Automatic Luminescence Analyser ALA1/4.......................................... 1 pce.
- Spare fuse ........................................................................................... 1 pce.
- Rotor for 0.5 ml tubes........................................................................... 1 pce.
- Rotor for 0.2 ml tubes........................................................................... 1 pce.
- Data Cable............................................................................................ 1 pce.
- Software installation disc...................................................................... 1 pce.
- Software installation manual................................................................ 1 copy
- Software operating manual.................................................................. 1 copy
- Software Quick Start............................................................................ 1 copy
- Operating Manual, Certificate.............................................................. 1 copy
3.3. Place the unit on a suitable working surface away from sunlight and bright
light other strong sources.
3.4. Connect the unit to the PC via USB port on the Analyser rear side using the
supplied data cable.
3.5. Connect the power cord to the mains power socket on the rear side of the
device.
3.6. Install the software (supplied separately) following the Software installation
and operation manual.

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4. Operation
4.1. Connect the power cord to the mains outlet.
4.2. Switch ON the power switch on the rear. The analyser turns on and the display
shows “ALA_X.XX.X”.
Attention! It is necessary to allow 15 min time for warming up before
conducting detection.
Attention! If the Analyser was turned off for longer period (more than 7
days), it is necessary to turn it on and allow long warm-up
time (about 8 h).
4.3. Operation with the software is described in the manual supplied with the
software (supplied separately).
4.4. Rotor installation and removal
Rotor removal. The rotor is secured with the latch (fig.1). Unscrew the latch
(anticlockwise) and remove the rotor.
Rotor installation. Install the rotor into the analyser. The positioning pin on
the base inside the unit must match into the opening in the rotor. Secure the
new rotor carefully and turning the latch tightly.
Caution! Do not operate the unit while the lid is open.
Caution! Remove the latch before taking out the rotor. Always secure
the rotor with the latch after installation.
Fig. 1. Latch
4.5. During operationm the following warning indication may appear on the
analyser display:
Exception 01
failure at filter initial positioning
Exception 02
failure at filter positioning for the given channel
Exception 04
the rotor not installed or installed in the wrong way. It can also
indicate the rotor position sensor failure.
4.6. At the end of operation, close the ALA_1 software saving the necessary data.
4.7. Turn OFF the mains switch on the rear. Unplug the unit from the mains outlet.

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5. Specifications
The unit is designed for operation in cold rooms, incubators and closed la-
boratory rooms at ambient temperature from +4°C to +40°C in a non-condensing
atmosphere and maximum relative humidity 80% for temperatures up to 31°C
decreasing linearly to 50% relative humidity at 40°C.
5.1. Number of detection channels..................................................................... 4
5.2. Detector ................................................................................. Photomultiplier
5.3. First Channel FAM
excitation wavelength ......................................................................................460 nm
emission wavelength .......................................................................................515 nm
5.4. Second Channel JOE, HEX, R6G
excitation wavelength ......................................................................................532 nm
emission wavelength .......................................................................................570 nm
5.5. Third Channel ROX
excitation wavelength ......................................................................................570 nm
emission wavelength .......................................................................................610 nm
5.6. Fourth Channel CY-5
excitation wavelength ......................................................................................630 nm
emission wavelength .......................................................................................670 nm
5.7. Rotor capacity
R-036................................................................................................36 x 0.5 ml tubes
R-048 ...............................................................................................48 x 0.2 ml tubes
5.8. Measurement time
(rotor R-036, 1 channel ) ......................................................................... 55 s
5.9. Excitation source ................ Light diode (Luxeon), through interference filter
5.10. Interference filter transmission spectrum half-width ...............................7 nm
5.11. Number of measurements per sample in detection mode .....................2000
5.12. Interface cable ........................................................................................USB
5.13. Input voltage ......................................................................... 230V; 50/60 Hz
5.14. Power consumption, no more than ........................................................30 W
5.15. Dimensions 400х250х190 mm
5.16. Weight*................................................................................................. 8.5 kg
Biosan is committed to a continuous program of improvement and reserves
the right to alter design and specifications of the equipment without additional
notice.
* Accurate within ±10%.

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6. Maintenance
6.1. If the unit requires maintenance, disconnect the unit from the electric circuit
and contact Biosan or your local Biosan representative.
6.2. All maintenance and repair operations must be performed only by qualified
and specially trained personnel.
6.3. Standard ethanol (75%) or other cleaning agents recommended for cleaning
of laboratory equipment can be used for cleaning and decontamination of
the unit.
6.4. Fuse replacement. Disconnect from the power supply socket. Remove the
power cable from the rear of the unit. Pull out the fuse drawer by applying
leverage in recess (A). Remove the fuse from the holder. Check and replace
with the correct fuse if necessary, 230V –M 630mA (type M- time lag: Me-
dium).

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7. Warranty and Claims
7.1. The Manufacturer guarantees the compliance of the unit with the require-
ments of Specifications, provided the Customer follows the operation, stor-
age and transportation instructions.
7.2. The warranted service life of the unit from the date of its delivery to the
Customer is 24 months (excluding the consumables, i.e. calibration kits).
Contact your local distributor to check availability of extended warranty.
7.3. Warranty covers only the units transported in the original package.
7.4. If any manufacturing defects are discovered by the Customer, an unsatisfac-
tory equipment claim shall be compiled, certified and sent to the local distrib-
utor address. Please visit www.biosan.lv, Technical support section to obtain
the claim form.
7.5. The following information will be required in the event that warranty or post-
warranty service comes necessary. Complete the table below and retain for
your records.
Model
Automatic Luminescence Analyser ALA1/4
Serial number
Date of sale

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SIA Biosan
Ratsupites 7, build. 2, Riga, LV-1067, Latvia
Phone: [phone]
Fax: [phone]
http://www.biosan.lv
Edition 2.01 –October 2015