Biosan DEN-1 User manual

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If you have any feedback on our products or services, we would like to hear from you.
Please send all feedback to:
Manufacturer
SIA Biosan
Rātsupites iela 7 k-2, Rīga, LV-1067, Latvija/Latvia
Phone: [phone]
Fax: [phone]
www.biosan.lv
Service e-mail: [e-mail]
Marketing e-mail: [e-mail]

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Contents
1. About this edition of user instructions.......................................................................3
2. Safety precautions ...................................................................................................4
3. General information..................................................................................................6
4. Getting started..........................................................................................................7
5. Operation .................................................................................................................8
6. Calibration..............................................................................................................10
7. Specifications.........................................................................................................11
8. Ordering information ..............................................................................................12
9. Care and maintenance...........................................................................................13
10. Storage and transportation.....................................................................................13
11. Warranty.................................................................................................................14
12. EU Declaration of conformity..................................................................................15
1. About this edition of user instructions
The current edition of the user instructions applies to the following models and ver-
sions of densitometers, suspension turbidity detectors:
Model and name
Version
DEN-1, densitometer, suspension turbidity detector
V.3AW
DEN-1B, densitometer, suspension turbidity detector
V.3AW

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2. Safety precautions
Caution!
Make sure you have fully read and understood the present user instruc-
tions before using the equipment. Please pay special attention to sections
marked by this symbol.
2.1. General safety.
•The protection provided can be ineffective if the operation of the appliance does not
comply with the manufacturer's requirements.
•Save the unit from shocks and falling.
•Store and transport the unit at ambient temperatures between -20°C and +60°C and
maximum relative humidity of 80%.
•After transportation or storage and before connecting it to the electric circuit, keep the
unit under room temperature for 2-3 hrs.
•Use only original parts and accessories, provided by manufacturer for this product.
•Before using any cleaning or decontamination methods except those recommended
by the manufacturer, check with the manufacturer that the proposed method will not
damage the equipment.
•Do not make modifications in design of the unit.
2.2. Electrical safety.
•Connect only to the mains with voltage corresponding to that on the serial number
label.
•Use only the external power supply provided with this product.
•Ensure that the power plug is easily accessible during use.
•Disconnect the unit from the mains before moving.
•If liquid penetrates into the unit, disconnect it from the mains and have it checked by
a repair and maintenance technician.
•Do not operate the unit in premises where condensation can form. Operating condi-
tions of the unit are defined in the Specifications section.
2.2.1. Batteries (only for model DEN-1B)
Caution!
Risk of fire and explosion!
•Use only AA size batteries, rechargable or non-rechargeable.
•Replace all used batteries in the unit at the same time. Insert batteries properly, with
the plus (+) and minus (–) terminals aligned correctly. In case of doubt, turn off the
unit immediately and check the polarity.
•Keep all batteries in a safe place away from children and pets.
•Where possible, recycle the batteries. Contact local government for information about
the disposal options in the area.
•Remove batteries from the unit if it will notbe used for severalmonths to avoid battery
leakage.
•Do not mix old and new batteries, batteries of different brands, or batteriesof different
types (e.g. zinc chloride and alkaline batteries) in the same unit as this may cause
the batteries to leak.
•Do not attempt to recharge non-rechargeable batteries. This can cause batteries to
overheat or leak.

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•Do not place batteries in a refrigerator. This will not ‘recharge’, increase storage life,
or increase batteries’ power.
•Do not exceed storage temperature, it reduces battery performance and may also
lead to leakage.
•Do not remove the battery label, or attempt to take the battery apart, or dispose of in
a fire as this may lead to rupture and/or chemical burns.
2.3. During operation.
•Do not operate the unit in environments with aggressive or explosive chemical mix-
tures. Please contact manufacturer for possible operation of the unit in specific at-
mospheres.
•Do not operate the unit if it is faulty or has been installed incorrectly.
•Do not use outside laboratory rooms.
•Use Select and Install buttons only for calibration of the unit. Pressing the buttons
can cause loss of calibration.
2.4. Biological safety.
•The user is responsible to carry out appropriate decontamination if hazardous mate-
rial spills on or penetrates into the equipment.

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3. General information
DEN-1 & DEN-1B densitometers are designed for solution turbidity measurement in
the range of 0.0 - 6.0 McFarland units or 0 cells/ml - 18x108cells/ml. DEN-1 & DEN-1B are
capable of measuring solution turbidity in a wider range, up to 15.0 McFarland units, how-
ever, it is necessary to remember that in this case the standard deviation values increase.
DEN-1 & DEN-1B densitometers are used for 1) determining concentration of cells
(bacterial, yeast cells) in the fermentation process, 2) detection of susceptibility of microor-
ganisms against antibiotics, 3) identification of microorganisms with various test systems, 4)
measuring optical density at fixed wavelength and 5) quantitative evaluation ofconcentration
of dyed solutions that absorb green light.
The operation principle is based on optical density measurement with digital result
representation in McFarland units.
The unit is calibrated at the factory and saves calibration data when being switched
off. However, it can be recalibrated by multiple points in 0.0–6.0 McF range if necessary. We
reccommend using our Biosan standards for opyimal results, but both commercial standards
and the cell suspensions prepared in the laboratory (e.g., BaSO4) can be used for calibration.
Table 1. Interpretation of McFarland Standard results into corresponding numeric values of
bacterial suspension concentration and their optical density at 550 nm.
McFarland
Standard
Composition
Interpretation
Concentration of
BaSO4
Bacterial concentration1
Theoretical optical
density2at 550 nm
0.5
2.40 x 10-5 mol/L
150 x 106cells/mL
0.125
1
4.80 x 10-5 mol/L
300 x 106cells/mL
0.25
2
9.60 x 10-5 mol/L
600 x 106cells/mL
0.50
3
1.44 x 10-4 mol/L
900 x 106cells/mL
0.75
4
1.92 x 10-4 mol/L
1200 x 106cells/mL
1.00
5
2.40 x 10-4 mol/L
1500 x 106cells/mL
1.25
6
2.88 x 10-4 mol/L
1800 x 106cells/mL
1.50
1
Bacterial concentration depends on microorganism size. The numbers represent an average value
valid for bacteria. For yeasts, which are larger, these numbers should be divided by about 30.
2
Values correspond to optical densities of bacterial suspensions. The BaSO4solutions optical density
values differ, because the particle size and form differ from those of bacteria and light is diffracted dif-
ferently

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4. Getting started
4.1. Unpacking. Remove packing materials carefully and retain them for future shipment
or storage of the unit. Examine the unit carefully for any damage incurred during
transit. The warranty does notcover in-transit damage. Warranty covers only the units
transported in the original package.
4.2. Complete set. Package contents:
4.2.1. Standard set:
- DEN-1 / DEN-1B Densitometer, suspension turbidity detector ........................1 pce.
- A-16 adapter for Ø16 mm tubes.......................................................................1 pce.
- AA batteries (for DEN-1B only)........................................................................3 pcs.
- External power supply .....................................................................................1 pce.
- User instructions, declaration of conformity ....................................................1 copy
4.2.2. Optional accessories, on request:
- A-12 adapter for Ø12 mm tubes.......................................................................1 pce.
- CKG16 calibration kit for Ø16 mm glass tubes ..................................................1 set
- Calibration kit for Ø18 mm glass tubes...............................................................1 set
- Calibration kit for Ø12 mm glass tubes (for DEN-1B only).................................1 set
- Glass sample tubes without lid, ........................................................ 1 set of 78 pcs.
Figure 1. View from below
4.3. Setup.
- (for DEN-1B) Battery installation. Pull back the pin on the battery compartment (fig.
1/2) on the underside and open lid. Insert batteries as shown on the scheme inside
and close the lid.
- Place the unit on horizontal even working surface.
- Connect the external power supply unit into the socket at the rear side of the unit
(fig. 3/2) and position the unit for an easy access to the external power supply and
the power switch.
Note.
For DEN-1B, connecting external power supply is optional when the bat-
teries are in use.
- Remove the protective film from the display.
4.4. Factory calibration. The device is pre-calibrated at the factory for operation with the
glass tubes 16 mm in external diameter (see the label on the bottom side of the unit,
fig. 1/1) at temperature range from +15°C to +25°C and saves calibration data when
being switched off.
Note.
Recalibrate the unit before using the tubes that are different from factory
calibrated (e.g. with different outer diameter, bottom shape or different
material such as plastic). See the Calibration section of this manual.

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5. Operation
5.1. Recommendations during operation.
•Remove the tube with the solution from the socket before switching the unit on or off.
•We recommended keeping the unit switched on for 15 minutes before starting the
operation in order to stabilize it in the working mode.
•If flat-bottomed tubes are used, the solution level should be higher than 7 mm from a
tube bottom; if round-bottomed tubes are used - higher than 12 mm from a tube bot-
tom.
•Check if the A-16 adapter is in the socket (fig. 2/1). The device is calibrated for oper-
ation with the glass tubes 16 mm in external diameter. To use different tubes, recali-
brate the unit, see the Calibration section of the manual.
Figure 2. Front view
Figure 3. Back view

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5.2. Connect the external power supply to electric circuit. Switch on the unit using the
Power switch (fig. 3/1) on the rear panel.
Note.
DEN-1B can operate without external power supply on battery power.
5.3. The following indication may be shown on the display (fig. 2/2):
•.–the unit is calibrated and ready for operation.
•LO BAT (DEN-1B) –low batteries, replace the batteries as described in 4.3 or con-
nect the external power supply.
Note.
The display of DEN-1B switches off if the socket is empty for longer that
one minute. Press the On key (fig. 2/2) to activate the unit.
5.4. Shake the tube with the solution. It is recommended to use a vortex for shaking, e.g.
Biosan V-1 plus.
5.5. To ensure measurement precision, check the external diameter of tubes. By default,
the unit works with Ø16 mm tubes, with A-16 adapter.
Caution!
Recalibrate the unit before using the tubes that are different from factory
calibrated (e.g. with different outer diameter, bottom shape or different
material such as plastic). See the Calibration section of this manual.
5.6. Insert the tube into the socket of densitometer (fig. 2/1). The McFarland value for the
solution will be shown on the display (fig. 2/3).
5.7. After finishing the operation switch off the unit using the Power switch (position O). If
the external power supply is used, disconnect it from electric circuit.

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6. Calibration
6.1. The unit is pre-calibrated at the factory for operation with the glass tubes 16 mm in
external diameter (see the label on the bottom side of the unit, fig. 1/1) at temperature
range from +15°C to +25°C and saves calibration data when being switched off. To
use different types of tubes, recalibrate the unit as shown below.
6.2. Before using the standards, prepare them by the instructions from the manufacturer.
6.3. Perform calibration from lower to higher calibration value. Use at least 2 points for
calibration. Different calibration pointsare available: 0.00, 0.50, 1.00, 2.00, 3.00, 4.00,
5.00, 6.00.
6.4. Connect the external power supply to electric circuit. Switch on the unit using the
Power switch (fig. 3/1) on the rear panel.
Note.
DEN-1B can operate without external power supply on battery power.
Caution!
Make sure that the tube socket is empty. For Ø12 mm or Ø16 mm tubes,
place the A-12 or A-16 adapters, accordingly.
6.5. Press the Select button (fig. 3/3) on the rear of the unit.
Note.
Use a thin pin of maximum diameter of 2 mm for pressing the Select and
Install buttons.
6.6. It is necessary to set values for an empty socket .and transparent standard ..
6.6.1. Empty socket. Display shows .indication. Press Install button (fig. 3/4) to save
empty socket value. Display shows next required calibration value.
6.6.2. Transparent standard. Display shows .indication. Insert standard for 0.00 value
into the socket (fig. 2/1) of the unit. If the standard for 0.00 value is not available, fill
the tube (of the kind that is used for operations) with distilled water. Use the tube as
the 0.00 value standard. Press Install button to save transparent standard value.
Display shows next required calibration value.

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Note.
Сalibrate the unit using as many points as possible to obtain precise results.
Minimum requirement are 2 points closest to the working range limits (e.g.
0.00 and 6.00 for operation in 0.00–6.00 McF range).
6.7. Common calibration rules. Display shows indication of necessary calibrationvalue.
Insert the necessary standard into the socket (fig. 2/1) of the unit and press the Install
button to save the value for current standard.
Note.
If pressing the Install button does not switch to the next standard value, it
means that the current standard in the socket has lower turbidity value than
the previous standard. Shake or replace the standard.
6.8. If a standard is not available, press the Select button to skip to the next calibration
value without recording the value.
6.9. Repeat steps 6.7-6.8 until the calibration is complete. After recording or skipping the
last value, unit automatically exits calibration mode and is ready for operation.
6.10. Reset to factory calibration. To reset the calibration of the unit to factory settings,
ensure that you are in the working mode and the socket of the unit is empty. Press
and hold Install key for 5 seconds. The unit displays a dot . , then changes it to ..
The values are now reset.
6.11. Switch off the unit using the Power switch (position O). If an external power supply
is used, disconnect it from electric circuit.
7. Specifications
The unit is designed for operation in cold rooms, incubators (except CO2incubators)
and closed laboratory rooms at ambient temperature from +4°C to +40°C in a non-condens-
ing atmosphere and maximum relative humidity 80% for temperatures up to 31°C decreasing
linearly to 50% relative humidity at 40°C.
Biosan is committed to a continuous programme of improvement and reserves the
right to alter design and specifications of the equipment without additional notice.
Light source....................................................................................................................LED
Wavelength .................................................................................................λ = 565 ± 15 nm
McFarland unit range...................................................................................0.00–15.00 McF
Resolution ..............................................................................................................0.01 McF
Accuracy, in the factory calibration range and of the full scale...................................... ±3%
Measurement time............................................................................................................1 s
Sample volume..............................................................................................2 mL minimum
External diameter of tubes........................12 mm (with A-12), 16 mm (with A-16) or 18 mm
Display........................................................................................................................... LCD
Dimensions (L x W x H).......................................................................... 165 x 115 x 75 mm
Weight, within ±10%....................................................................................................0.7 kg
Power consumption..................................................................................................... 0.1 W
Input voltage and current.....................................................................................12 V, 7 mA
External power supply.................................input AC 100-240 V, 50/60 Hz; output DC 12 V
Batteries (only DEN-1B) ............................................................................................3 x AA

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8. Ordering information
8.1. Models and versions available:
Model
Version
Catalogue number
DEN-1
V.3AW
BS-050102-AAF
DEN-1B
V.3AW
BS-050104-AAF
8.2. To inquire about or order the optional accessories or the replacement parts, contact
Biosan or your local Biosan representative.
8.3. Optional accessories:
Description
Catalogue number
A-12, adapter for Ø12 mm tubes
BS-050102-IK
CKG16, calibration kit for Ø16 mm glass tubes. Latex particles
BS-050102-BK
Calibration kit for Ø12 mm glass tubes. Polymer particles
On request
Calibration kit for Ø18 mm glass tubes. BaSO4suspension
On request
Glass sample tubes without lid, Ø16x100x0.8 mm, 78 pcs.
Valid for factory-calibrated units.
BS-050102-LK
8.4. Replacement parts:
Description
Catalogue number
A-16, adapter for tubes of 16 mm in external diameter
BS-050102-AK

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9. Care and maintenance
9.1. Service.
9.1.1. If the unit is disabled (e.g., no reaction to tube insertion or key presses, etc) or re-
quires maintenance, disconnect the unit from the mains and contact Biosan or your
local Biosan representative.
9.1.2. All maintenance and repair operations (except listed below) must be performed only
by qualified and specially trained personnel.
9.1.3. Operating integrity check. If the unit follow the procedure described in sections Op-
eration and Calibration, then no additional checks are required.
9.2. Cleaning and disinfection.
9.2.1. Use mild soap and water with a soft cloth or sponge for cleaning the exterior. Rinse
remaining washing solution with distilled water. Wipe dry the excess water with clean
soft cloth or sponge.
9.2.2. To disinfect the plastic parts, use 75% ethanol or DNA/RNA removing solution (e.g.
Biosan PDS-250). After disinfecting it is necessary to wipe the surfaces dry.
9.2.3. Internal (optical parts). Do not use liquids to clean optical parts. Use air from a rubber
siphon to blow away any particles.
9.2.4. The unit and its accessories are not autoclavable.
9.3. Decomissioning of the unit. The user is responsible for the decontamination of the
unit before decommissioning. Dispose of the appliance as electronic equipment in
accordance with the relevant national laws.
10. Storage and transportation
10.1. Store and transport the unit in a horizontal position (see package label) at ambient
temperatures between -20°C and +60°C and maximum relative humidity of 80%.
10.2. After transportation or storage and before connecting it to the electric circuit, keep the
unit under room temperature for 2-3 hrs.
10.3. For extended storage of the DEN-1B model, remove the batteries (see 4.3). The
DEN-1 model does not require special procedures.

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11. Warranty
11.1. The Manufacturer guarantees the compliance of the unit with the requirements of
Specifications, provided the Customer follows the operation, storage and transporta-
tion instructions.
11.2. The warranted service life of the unit from the date of its delivery to the Customer is
24 months. Extended storage does not extend the warranty. For extended warranty,
see 11.5.
11.3. Warranty covers only the units transported in the original package.
11.4. If any manufacturing defects are discovered by the Customer, an unsatisfactory
equipment report shall be compiled, certified and sent to the local distributor address.
To obtain the claim form, visit Technical support page on our website at link below.
11.5. Extended warranty.
•For DEN-1B, the Premium class model, one year of extended warranty is available
free of charge after registration, during 6 months from the date of sale. Online regis-
tration form can be found in section Warranty registration at the link below.
•For DEN-1, the Basic Plus class model, extended warranty is a paid service. Contact
your local Biosan representative or our service department through the Technical
support section on our website at the link below.
11.6. Description of the classes of our products is available in the Product class descrip-
tion section on our website at the link below.
Technical support
Registration
Product class description
biosan.lv/en/support
biosan.lv/register-en
biosan.lv/classes-en
11.7. The following information will be required in the event that warranty or post-warranty
service comes necessary. Complete the table below and retain for your records.
Model
Serial number
Date of sale
DEN-1, DEN-1B,
McFarland Densitometer

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12. EU Declaration of conformity
Unit type Densitometers
Models DEN-1, DEN-1B, DEN-600
Serial number 14 digits styled XXXXXXYYMMZZZZ, where XXXXXX is model code,
YY and MM –year and month of production, ZZZZ –unit number.
Manufacturer SIA BIOSAN
Latvia, LV-1067, Riga, Ratsupites 7 k-2
The objects of the declaration described above is in conformity with the following
relevant Union harmonization legislations:
LVD 2014/35/EU
LVS EN 61010-1:2011 Safety requirements for electrical
equipment for measurement, control, and laboratory use.
General requirements.
EMC 2014/30/EU
LVS EN 61326-1:2013 Electrical equipment for measurement,
control and laboratory use. EMC requirements.
General requirements.
RoHS3 2015/863/EU
Directive on the restriction of the use of certain hazardous
substances in electrical and electronic equipment.
WEEE 2012/19/EU
Directive on waste electrical and electronic equipment.
I declare that the Declaration of Conformity is issued under sole responsibility of the
manufacturer and belongs to the above-mentioned objects of the declaration.
Svetlana Bankovska
Managing director
__________________
Signature
__________________
Date

Biosan SIA
Rātsupites iela 7 k-2, Rīga, LV-1067, Latvija/Latvia
Phone: [phone] Fax: [phone]
http://www.biosan.lv
Edition 3.01 –May of 2021