Endress+Hauser Analytik Jena CyBio FeliX User manual

CyBio FeliX
Operating instructions

Registered trademarks:
CyBio ..Analytik Jena GmbH, Germany
Title to all other trademarks or brands which are referenced in this User Manual
belongs to their legal owners.
Copyright Copyright: 2022 Analytik Jena GmbH
All rights reserved. No part of this documentation may be dupli-
cated, photocopied, saved to a storage system or transferred to
electronic media without prior written permission of the pub-
lisher.
Manufacturer
Analytik Jena GmbH
Konrad-Zuse-Straße 1
07745 Jena, Germany
Germany
Tel: [phone]
Fax:[phone]
Service support
Tel: [phone]
E-Mail: [email protected]
Order number 30-5015-100-24BLE
Document type Translation of Original instructions
Serial number Refer to product nameplate

Rev. B 2022/02 III
CyBio FeliX

IV Rev. B 2022/02
CyBio FeliX

Rev. B 2022/02 V
CyBio FeliX
EC Declaration of Conformity (reproduction of the content)
Name and address of the manufacturer:
Analytik Jena GmbH
Konrad-Zuse-Straße 1
D-07745 Jena
Herewith we declare, that the product described below
CyBio FeliX
30-5015-100-24
30-5015-600-24
30-5015-125-25
is complying with all corresponding requirements of the Machinery Directive 2006/42/EC.
In addition the product is in conformity with the EC Directive relating to electromagnetic
compatibility 2014/30/EU. The safety objectives of the Low-Voltage Directive are taken
into account.
Harmonized Standards used:
EN ISO 12100 - Safety of Machinery - General principles for design - Risk assessment and
risk reduction
EN 61010-1 - Safety requirements for electrical equipment for measurement, control and
laboratory use
The person authorized to compile the relevant technical documentation:
Analytik Jena GmbH, Konrad-Zuse-Straße 1, D-07745 Jena

VI Rev. B 2022/02
CyBio FeliX
EC Declaration of Conformity (reproduction of the content)
Name and address of the manufacturer:
Analytik Jena GmbH
Konrad-Zuse-Straße 1
D-07745 Jena
Herewith we declare, that the product described below
CyBio FeliX
30-5015-500-25
is complying with all corresponding requirements of the Machinery Directive 2006/42/EC.
In addition the product is in conformity with the EC Directive relating to electromagnetic
compatibility 2014/30/EU. The safety objectives of the Low-Voltage Directive are taken
into account.
Harmonized Standards used:
EN ISO 12100:2011 - Safety of Machinery - General principles for design - Risk assess-
ment and risk reduction
EN 61010-1:2011 - Safety requirements for electrical equipment for measurement, con-
trol and laboratory use
The person authorized to compile the relevant technical documentation:Analytik Jena
GmbH, Konrad-Zuse-Straße 1, D-07745 Jena

Rev. B 2022/02 VII
CyBio FeliX
C E R T I F I C A T E
No. U8 037158 0008 Rev. 00
Page 1 of 1
TÜV SÜD America Inc. • 10 Centennial Drive • Peabody • MA 01960 • USA
Holder of Certificate: Analytik Jena GmbH
Konrad-Zuse-Str. 1
07745 Jena
GERMANY
Production
Facility(ies):
037158
Certification Mark:
Product:
Laboratory equipment
Model(s):
CyBio FeliX
Parameters:
Rated voltage: 100-240 VAC
Rated current: 2 A
Rated fraquency: 50/60 Hz
Protection class: I
Tested
according to:
UL 61010-1:2012/R:2018-11
CAN/CSA-C22.2 No. 61010-1:2012/A1:2018-11
UL 61010-2-081:2019
CAN/CSA-C22.2 No. 61010-2-081:2019
This product was voluntarily tested to the relevant safety requirements referenced on this certificate.
It can be marked with the certification mark above. The mark must not be altered in any way. This
product certification system operated by TÜV SÜD America Inc. most closely resembles system 3 as
defined in ISO/IEC 17067. Certification is based on the TÜV SÜD "Testing and Certification
Regulations". TÜV SÜD America Inc. is an OSHA recognized NRTL and a Standards Council of
Canada accredited Certification body.
Test report no.:
028-713039674-100
Date,
2020-12-15
( Abdul Sabbagh )

VIII Rev. B 2022/02
CyBio FeliX

Rev. B 2022/02 IX
CyBio FeliX
Table of Content
1 General information................................................................................ 1
1.1 Notes .......................................................................................................................1
1.2 Target group ...........................................................................................................1
1.3 Conventions .............................................................................................................2
1.3.1 Text marks............................................................................................................... 2
1.3.2 Design of notes ....................................................................................................... 3
1.4 Intended use ............................................................................................................4
1.5 Conforming Use .......................................................................................................4
1.6 Warranty & Liability ................................................................................................6
1.7 Scope of delivery .....................................................................................................6
2 Technical Specifications ........................................................................... 7
2.1 General data ............................................................................................................7
2.2 Overview operating data/conditions .....................................................................9
3Safetyinstructions
................................................................................. 11
3.1 General ................................................................................................................. 11
3.2 Standards & Directives ......................................................................................... 12
3.3 Safety markings ................................................................................................... 13
3.4 Danger areas ........................................................................................................ 16
3.5 Protective devices ................................................................................................ 17
3.6 Requirements On Personnel ................................................................................ 17
3.7 Safety Requirements for Transportation & Installation ..................................... 17
3.8 Safety Notes for Operation .................................................................................. 18
3.8.1 General.................................................................................................................. 18
3.8.2 Explosion Protection, Fire Prevention ................................................................. 18
3.8.3 Electrics ................................................................................................................. 18
3.8.4 Basic Maintenance & Care.................................................................................... 18
3.8.5 Handling of Dangerous Substances ..................................................................... 19
3.8.6 Chemical Resistance ............................................................................................. 20
3.9 Rules of conduct in case of Emergency ............................................................... 23
4 Technical description............................................................................. 25
4.1 Set-up/components ............................................................................................. 25
4.1.1 CyBio FeliX - Overview.......................................................................................... 25
4.1.2 Type plate.............................................................................................................. 27
4.1.3 Enclosure and louver ............................................................................................ 27
4.1.4 Decks ..................................................................................................................... 28
4.1.5 Operating status indicator.................................................................................... 31
4.1.6 Head mount .......................................................................................................... 33
4.1.7 Pipetting heads (Pipetting heads)/versions*...................................................... 34
4.1.8 Fan......................................................................................................................... 34
4.1.9 Connector panel.................................................................................................... 35
4.2 Working mode ...................................................................................................... 37
4.3 Operating Modes ................................................................................................. 37
5 Packing, transport and storage............................................................... 39
5.1 Safety Notes ......................................................................................................... 39
5.2 Packaging ............................................................................................................. 40
5.2.1 Attach transport locks........................................................................................... 40
5.2.2 Packing the device ................................................................................................ 41

X Rev. B 2022/02
CyBio FeliX
5.2.3 Storage .................................................................................................................. 41
6Start-up
............................................................................................... 43
6.1 Site requirements ................................................................................................. 43
6.1.1 Installation Requirements .................................................................................... 43
6.1.2 Spatial Requirements............................................................................................ 43
6.1.3 Power Supply ........................................................................................................ 44
6.2 Initial start-up and configuration ........................................................................ 45
6.3 Function Tests ...................................................................................................... 46
6.3.1 Precision test......................................................................................................... 46
6.3.2 Accuracy Test ........................................................................................................ 46
6.3.3 Leak Test ............................................................................................................... 46
7Operation
............................................................................................. 47
7.1 Switching on (operational readiness) ................................................................. 47
7.2 Establish operation after abort (e.g. due to power failure) ............................... 48
7.3 Changing the pipetting head ............................................................................... 49
7.3.1 Insert the pipetting head...................................................................................... 50
7.3.2 Removing the pipetting head............................................................................... 51
7.4 Manual operation ................................................................................................ 52
7.4.1 Axes of motion...................................................................................................... 52
7.4.2 Move the pipetting head in the Z direction......................................................... 53
7.5 Deck Configuration .............................................................................................. 55
7.6 Software-controlled operation ............................................................................ 56
7.6.1 General commands (overview) ............................................................................ 56
7.6.2 Attach/remove transport lock .............................................................................. 58
7.6.3 Preparation of head change / switch off ............................................................. 59
7.6.4 Movements in X, Y and Z directions..................................................................... 60
7.6.5 Tip pick-up/change - pipetting head T................................................................ 62
7.6.6 Tip pick-up/change - pipetting head R................................................................ 63
7.6.7 Pipetting................................................................................................................ 67
7.7 Turning off ........................................................................................................... 68
7.8 Troubleshooting ................................................................................................... 69
7.9 Safety notes .......................................................................................................... 69
7.10 Device not working .............................................................................................. 69
7.10.1Further errors........................................................................................................ 69
7.11 Error messages by software ................................................................................. 70
7.12 Troubleshooting - Instructions ............................................................................ 72
7.12.1Changing the device fuse...................................................................................... 72
7.12.2LED flashing – red................................................................................................. 73
7.12.3Establish operation after aborts (e.g. due to power failure) .............................. 73
7.12.4Manual axis movement after an abort ................................................................ 73
8 Maintenance & Care .............................................................................. 75
8.1 Safety Notes ......................................................................................................... 75
8.2 Maintenance Work .............................................................................................. 76
8.2.1 Overview................................................................................................................ 77
8.3 Maintenance/testing/cleaning -
Instructions 77
8.3.1 Cleaning the basic unit (including decks) ............................................................ 79
8.3.2 Service the head mount........................................................................................ 80
8.3.3 Cleaning cones ...................................................................................................... 80
8.3.4 Check/replace the O-rings.................................................................................... 81
8.3.5 Check the function of the louver .......................................................................... 83
9 Shutting Down...................................................................................... 85

Rev. B 2022/02 XI
CyBio FeliX
10 Accessories and Spare parts ................................................................... 87
10.1 Accessories ........................................................................................................... 87
10.1.1Pipette tips ............................................................................................................ 87
10.2 Pipetting Heads .................................................................................................... 88
10.3 Liquid handling /CHOICE adapter ....................................................................... 89
10.4 Holder ................................................................................................................... 89
10.5 Further Accessories .............................................................................................. 90
10.6 Spare parts ........................................................................................................... 90
11 Disposal ............................................................................................... 91
11.1 Consumables ........................................................................................................ 91
11.2 Reagents ............................................................................................................... 91
11.3 System & Accessories ........................................................................................... 91

XII Rev. B 2022/02
CyBio FeliX

Rev. B 2022/02 XIII
CyBio FeliX
Table of Figures
Fig. 1: Certification mark on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Fig. 2: Warning signs on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Fig. 3: Warning sign on pipetting head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Fig. 4: Danger areas. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Fig. 5: Version with louver/enclosure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Fig. 6: Version without louver/enclosure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Fig. 7: Type plate for the version with enclosure/louver . . . . . . . . . . . . . . . . . . . . . . . . 27
Fig. 8: Type plate for the version without enclosure/louver . . . . . . . . . . . . . . . . . . . . . 27
Fig. 9: Deck positions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Fig. 10: Heights – deck A, B and C . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Fig. 11: Heights – deck A, B and C . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Fig. 12: LED – Operating status indicator/ Arrow keys . . . . . . . . . . . . . . . . . . . . . . . . . 31
Fig. 13: LED – Operating status indicator/button . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Fig. 14: Head mount . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Fig. 15: Rear of the device (rear panel removed, fan marked) . . . . . . . . . . . . . . . . . . . 34
Fig. 16: Connectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Fig. 17: Connectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Fig. 18: Power switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
Fig. 19: Head mount (clamping lever swiveled forward). . . . . . . . . . . . . . . . . . . . . . . . 50
Fig. 20: Push on the pipetting head. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Fig. 21: Pipetting head ready for operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Fig. 22: Pipetting head (fastened). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Fig. 23: Take off the pipetting head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Fig. 24: Head retainer (clamping lever swiveled forward) . . . . . . . . . . . . . . . . . . . . . . 51
Fig. 25: Axes of motion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Fig. 26: Commands . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Fig. 27: Settings (Set tool information). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Fig. 28: Settings for depositing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Fig. 29: Component window. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Fig. 30: Attach transport lock . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Fig. 31: Window: Positioning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
Fig. 32: Interactive positioning for setting an offset . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
Fig. 33: Component window. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Fig. 34: Method for manual change . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Fig. 35: Component window. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Fig. 36: Example: Pick up CyBio RoboTipTray. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Fig. 37: Component window. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
Fig. 38: Example: Pick-up of CHOICE adapters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
Fig. 39: Method Pick up pipette tips (example) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
Fig. 40: Component window. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
Fig. 41: Example of setting down tips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
Fig. 42: Depositing an LH adapter (if necessary with tips, make sure to use
a suitable holder). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
Fig. 43: Course of events. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Fig. 44: Course of events. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Fig. 45: Fuses (2x) in the combination element at the rear of the device. . . . . . . . . . 72
Fig. 46: Cover magazine (transport protection) attached . . . . . . . . . . . . . . . . . . . . . . . 79
Fig. 47: Head mount . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
Fig. 48: Safety workbench incl. door monitoring set . . . . . . . . . . . . . . . . . . . . . . . . . . . 96
Fig. 49: Door monitoring set. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
Fig. 50: Safety workbench. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 98
Fig. 51: Safety light curtain. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99
Fig. 52: Pin assignment for the ESTOP IN connection . . . . . . . . . . . . . . . . . . . . . . . . . 102

XIV Rev. B 2022/02
CyBio FeliX

Rev. B 2022/02 1
CyBio FeliX General information
1 General information
1.1 Notes
These operating instructions provide information on the design and function of the device
and qualified operating personnel with the necessary knowledge for safe operation.
Furthermore, the operating instructions contain information on the care of the device and
the maintenance scope provided by the manufacturer.
In addition, you can use the operating instructions to analyze possible causes of malfunc-
tions and determine suitable measures for eliminating them.
The operating instructions must be accessible to the operating and maintenance personnel
at all times.
1.2 Target group
These operating instructions are aimed at:
Qualified and trained personnel who operate and maintain the equipment
(→ „Requirements On Personnel” on page 13).
Employees responsible for
– the planning of procedures,
– maintenance and cleaning work,
– safety devices etc.
NOTE
All information represents the state of knowledge at the time of printing.
The manufacturer reserves the right to make changes if deemed necessary in the inter-
est of technical progress.

2 Rev. B 2022/02
General information CyBio FeliX
1.3 Conventions
1.3.1 Text marks
Instructions for actions which occur in chronological order are numbered, combined in
action units
and provided with the corresponding result.
Lists which are not in chronological order are shown as itemized lists, sub-listings or
as bullet points.
Safety instructions are indicated by pictographs and a signal word (→ „Design of notes” on
page 3).
Action-related safety notes always precede the corresponding actions.
Commands, buttons, text fields, check boxes and the like are marked as follows - example
command "Load".
Optional equipment components/versions are marked with an *.
Please refer to the following table for the design of the cross-references:
Table 1: Design of the cross-references
Type of cross-reference Display Comment
Reference to an illustra-
tion →see Fig. 4 Illustration is located
on the same page
Reference to an illustra-
tion and page → see Fig. 4 on page 12 Illustration is located
on another page
Reference to a position
(of an illustration) Pos. 2, →see Fig. 5 The position of the specified
illustration is referenced –
Illustration is located
on the same page
Reference to a position
(of an illustration
and page)
Pos. 2,
→ see Fig. 5 on page 25
The position of the specified
illustration is referenced –
Illustration is located
on another page
Reference to a page → page 1 Page reference
Reference to a heading
and page → „Conventions” on page 2 Page reference with added
headline
Reference to a table and
page → see Table 6 on page 10 Reference to a table

Rev. B 2022/02 3
CyBio FeliX General information
1.3.2 Design of notes
WARNING
Indicates a potentially hazardous situation which,
if not prevented, can result in death or most serious injuries.
CAUTION
Dangerous situation!
Potential consequences: Mild or minor injuries.
NOTE
Dangerous situation!
Potential consequences: Material damage!
TIP
Useful application tips, no risk or hazard.
TIP
Notes on environmental protection!

4 Rev. B 2022/02
General information CyBio FeliX
1.4 Intended use
The device is a simultaneous pipetting system (depending on model version or pipetting
head
from 1 to a maximum of 384 channels) and intended for the automatic processing of
microplates.
The system's range of applications is limited by the functional scope of available software
and firmware and by the scope of delivery. Users are therefore required to operate this sys-
tem in no way or mode other than described or specified in these operating instructions.
1.5 Conforming Use
The pipettor is designed for automatic processing of microplates in chemical and biological
laboratories.
In the fields of medicine and diagnostics, its use is limited to research.
The basic functions are to aspirate and dispense fluids into and out of microplates, reser-
voirs, (columns, individual wells and tubes).
The term conforming use of the device presumes that:
the system is operated by qualified and trained research and laboratory personnel
all operating requirements quoted in this User Manual and described procedural
sequences and related safety notes are duly observed
all specifications in this Manual regarding system start-up, operation, preventive main-
tenance and care are met
applicable safety standards or rules are always fulfilled.
Any type of mode of application other than specified herein is considered non-conforming
use!
The user will be solely liable for any damage resulting from a non-conformance.

Rev. B 2022/02 5
CyBio FeliX General information
The term non-conforming use will include, but is not limited to:
the use of the device in medical laboratories not affiliated with research
working with explosive or aggressive substances
working in explosive atmospheres - use in potentially explosive areas is not permitted
This includes compliance with all safety requirements for the protection of people and
material goods during work with radioactive, infectious, poisonous, corrosive, combustible
and other hazardous substances. The user is under obligation to fulfill all requirements on
laboratory equipment and the conduct of personnel handling substances of this nature and
on the practices , in place for cleanness, sterilization, environmental protection and waste
disposal.
For the use of the device with hazardous substances, the user is recommended - unless
otherwise regulated by law - to issue operating instructions.
Accordingly, this User Manual contains no safety notes to protect from personal injury or
material damage caused by substances being examined.
Process control must rely on included manufacturer software.
Alterations in or damage to the software may give rise to faults in process flow and dam-
age the system or its components.
Software protection is the sole responsibility of the user.
NOTE
System operation with involvement of hazardous substances will be the sole responsi-
bility of the user!

6 Rev. B 2022/02
General information CyBio FeliX
1.6 Warranty & Liability
The duration of the warranty as well as the liability correspond to the legal
regulations as well as the regulations in the general terms and conditions of the manufac-
turer.
Warranty will be limited to repair services or replacement of damaged parts. It will exclude
consequential damage of any kind. Damage to wearing parts is not included in the war-
ranty.
Any deviation from conforming use as defined in this User Manual (operating require-
ments, process sequences) will result in restricted acceptance of warranty or liability claims
in the event of damage.
Warranty and liability claims for personal injury and property damage are excluded if the
device is not operated in accordance with section
→ „Conforming Use” on page 4.
1.7 Scope of delivery
The scope of delivery of the device - depending on the model version- consists of:
CyBio FeliX
- Basic unit or Workstation1
-Pipettinghead*
Accessories
-Scopeof→ „Accessories” on page 87
Cable
- Mains connection cable
-USBcable
Documentation
- Operating instructions
- Translation of the original operating instructions*
- EC Declaration of Conformity
- Accessories catalog*
Packaging
-Packingbox
- Transport locks
NOTE
This loss-of-warranty clause shall apply to potential periods of interruption in business
and to any system component that had not been directly affected by authorized warran-
ty work.
1 Or version without enclosure/louver.