Endress+Hauser Analytik Jena UVP DigiDoc-It Guide

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UVP DigiDoc-It®Imaging System
Installation and User Instructions
Analytik Jena US Analytik Jena AG
2066 W. 11th Street Konrad-Zuse-Str. 1
Upland, CA 91786 07745 Jena, Germany
Phone: [phone] Phone: + 49 3641 77 7407
Fax: [phone] Fax: [phone]
Web Site: us.analytik-jena.com

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Introduction
The UVP DigiDoc-It Imaging System is a basic gel imaging system designed to fit over any of Analytik Jena’s optional
UVP Transilluminator PLUS or optional UVP Transilluminator and drawer combination. The system combines:
Hood enclosure
DigiCam 125 digital color camera
Ethidium Bromide (EtBr) Filter
VisionWorks®Acquisition and Analysis Software (refer to software manual for installation)
UV transilluminator or drawer assembly (optional). Transilluminator instructions are provided in a
separate manual.
Hood Enclosure
The DigiDoc-It hood enclosure includes a cap to reduce ambient and
reflective light when taking pictures. The soft rubber viewport window
allows researchers to view samples prior to capturing images. The slide
closure under the viewport minimizes ambient light when taking pictures.
The lightweight door provides access to the enclosure interior for
placement of samples on the transilluminator surface.
The DigiDoc-It drawer fits under the hood enclosure and accommodates a compact UV Transilluminator.
Camera cap
enclosure for access
to the camera
Handles for carrying
the hood
Gel Viewport
Window
Hood access door
DigiCam 125 camera
Ethidium Bromide Filter is
located inside the hood
under the camera
Viewport cover
UVP Transilluminator
PLUS
DigiDoc-It drawer
(Note: color scheme is different)

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Camera & UV Filter
The digital color camera includes a zoom lens.
The EtBr filter, orange-colored UV blocking band pass filter, is installed inside the hood at the Analytik Jena factory.
The filter is used to absorb UV and IR radiation from the transilluminator and to enhance the orange/pink bands
generated by Ethidium Bromide stained gels. The filter can be removed when imaging non-fluorescent media
(protein gels, colony plates, etc.) in order to produce brighter images.
VisionWorks® Software
The VisionWorks® software loads onto the user’s existing computer. The software controls the camera acquisition
functions as well as enhancement and reporting features. For information on installation and use of the software,
refer to the VisionWorks Software help files.
UV Transilluminator
A UV transilluminator may be shipped with the system. The transilluminator includes an ultraviolet blocking cover and
the cover should be removed when the transilluminator is used with the system. Refer to the Transilluminator manual
for separate instructions.
Operating System Requirements
Windows 7, 8, 10
Intel Pentium Processor or equivalent, 1.6 GHz or higher
1 GB of RAM or greater
200 MB of available hard disk space for the program, more for data
One Universal Serial Bus (USB) for connecting the camera
Color monitor, supporting at least 1024 x 768 resolution and 16-bit or better colors; 24-bit or 32-bit color is
strongly recommended
NOTE: For 21 CFR Part 11 support functionality, the computer partition must be formatted with NTFS. Refer to the
VisionWorks® software manual for instructions.

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UVP Transilluminator PLUS
(shown with UV blocking cover)
DigiDoc-It Drawer
(Note: different color scheme and style)
System Setup
DO NOT ATTEMPT TO CONNECT ANY WIRING WHILE THE EQUIPMENT IS CONNECTED TO ANY POWER SUPPLY.
CAUTION: Do not install the system in places with high moisture, dust or high temperature. Do not use any oil or
petroleum based cleaner for the cabinet. Use only mild soap or detergent solution for cleaning. Ensure that the
system is turned OFF during cleaning. Keep the equipment away from motors or other large magnetic equipment
apparatus.
Place the Hood on top of the Transilluminator
The Hood Enclosure can be used with a UVP Transilluminator PLUS. When using a UVP Transilluminator, the optional
drawer is required.
CAUTION: All Analytik Jena Transilluminators are powerful sources of UV radiation that will cause damage to
unprotected eyes and skin. Before operating any unit, be sure all personnel in the area are properly protected. UV
Blocking Eyewear is highly recommended and should be worn. Refer to the Accessories section of this manual for UV
Blocking Eyewear.
UVP Transilluminator PLUS
1. Remove the UV blocking clear cover from the Transilluminator.
2. Position the transilluminator so the switch is facing the user.
3. Connect the power cord to the back of the transilluminator and
connect the plug to a power source.
4. Place the Hood Enclosure on top of the Transilluminator surface so
that the filter glass is covered.
UVP Transilluminator and Drawer
1. Remove the UV blocking clear cover from the Transilluminator.
2. Place the Transilluminator into the drawer.
3. Connect the power cord to the back of the transilluminator and
push the plug through the access hole in the back of the drawer.
4. Connect the plug to the power source.
5. Access to the transilluminator switch is via the access port on the
front of the drawer.
6. Place the DigiDoc-it hood over the drawer.
Camera Assembly
1. The camera will be installed in the DigiDoc-It hood at the Analytik
Jena factory.
2. To connect the power cable to the camera, thread the camera’s
power cable through the round notched hole from the back of the
camera cap and plug it into the camera as shown.
3. To connect the USB cable to the camera, thread the camera’s USB cable through the round notched hole
from the back of the camera cap and plug into the camera. Leave the other end unplugged until ready to
load the software.
4. Plug the camera’s power supply into a wall outlet or surge-protector receptacle.

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Note: The Ethidium Bromide filter is installed inside the hood enclosure at the Analytik Jena factory. If the filter
needs to be replaced, turn the hood upside down. Loosen screws and slide the filter into the filter tray. Retighten
screws and place the hood upright.
Install the VisionWorks® Software
NOTE: The following provides a brief instruction for installing the software. For additional instruction on using the
software, refer to the VisionWorks help files.
1. Insert the VisionWorks flash drive into the computer.
2. Navigate to the flash drive folder and open the folder.
3. Open the first folder found in the drive and then open the Installation folder.
4. Scroll to find the SetupVisionWorks.exe file and double click to run installation.
5. The first window will be a welcome to prerequisites screen. Click OK, Next. Leave all options in their
default settings and click Next. Proceed through the prerequisite driver installations. Once complete,
VisionWorks® installer will open, where the user can click INSTALL and proceed until complete.
Connect the Camera to the Computer
NOTE: It is recommended to only connect the camera to the computer while the camera is off.
1. Connect the USB cable from the camera to the computer.
2. Turn the camera on and select “M” on the camera dial.
NOTE: Do not use the VisionWorks® software while the camera dial is set to movie mode as doing
so may result in an error.
Hole in DigiDoc-It
hood for cables
Power supply cable,
plug into wall outlet
USB cable, plug into
computer
DigiCam 125 camera
(may appear different
than shown)

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Set camera to M
Connect to VisionWorks® Software
1. Open the VisionWorks software. The software should automatically identify the camera. The camera
control window should be active and ready to capture images.

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System Operation
Loading Gels into the Darkroom Hood
1. Turn Off the UV Transilluminator.
2. Open the darkroom door.
3. Place the sample to be imaged in the center of the transilluminator filter glass.
4. Close the darkroom door.
5. Turn On the transilluminator.
Note: It is important to minimize the light source exposure to the sample. Turn off the light source as soon as the
image is captured.
View a Sample Prior to Capture
1. Open the Viewport Window on the hood by sliding the cover to the right.
2. After visually checking that the sample is loaded correctly, close the Viewport Window. Otherwise, light from
the viewport will interfere with the capturing process.
Camera Operation
NOTE: Do not use the VisionWorks® software while the camera dial is set to movie mode as doing so may
result in an error.
1. To use the camera, open the camera cap on top of the hood.
2. To capture in automatic mode, set the camera mode dial button to A-DEP. (Automatic mode is
recommended for a quick image capture)
3. Close and secure the camera cap on the hood.
Note: If the camera is off when starting VisionWorks® software, a message stating that the application
cannot find a camera will appear. To remedy this, turn the camera on and click Retry.
Software Operation
NOTE: Do not use the VisionWorks® software while the camera dial is set to movie mode, as doing so may
result in an error.
1. Ensure that the camera mode is set to A-DEP for automated capture.
2. Open the VisionWorks® software.
3. A window displaying the camera options should appear as pictured below. If not, click on the
Acquisition Action tab and then on the Camera menu button.

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Camera Control Window
4. Select Preview at the top of the software to preview the image.
5. While in preview mode, the Brightness slider can be adjusted to brighten dark images.
6. The user may adjust the focus manually to enhance the appearance of the previewed image. Click the
Manual box on the Preview window, then adjust the focus settings.
Preview Window with Auto Focus
7. When satisfied with the previewed image, select Capture at the top of the software.

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Optional Camera Setup (Image Acquisition)
If operating the camera in automatic mode is not sufficient for the application, try utilizing the manual mode to gain
additional camera control.
1. Access the camera and turn the camera dial to M, the next position on the dial.
Set camera to M
2. A new camera window appears with additional options:
Camera Control in Manual Mode
3. Select the desired options for an enhanced image. See the camera manual for detailed descriptions of
options.
4. Select Capture at the top of the software.

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Service Procedures
Return Procedure
A Returned Goods Authorization (RGA) number must be obtained from Analytik Jena Customer Service
before returning any product.
Replacement Parts and Accessories
Accessories are available for use with the DigiDoc-It Imaging Systems. Contact Analytik Jena’s offices at the
telephone numbers below for replacement parts for DigiDoc-It equipment. For transilluminator replacement
parts, refer to the separate manual for the transilluminators included with your shipment.
Description Part Numbers
UVP Digidoc-it Drawer (for UVP Transilluminators) 98-0068-01
VisionWorks® Acquisition and Analysis Software 81-0266-01
UVP Thermal Printer, Digital (115/230V) 89-0069-06
UVP Thermal Printer, Digital (230V) 89-0069-07
Thermal Printer, Digital (230V for Europe) 98-0069-15
UVP Thermal Paper, 4 rolls (800 images) 89-0038-01
Thermal paper, 16 rolls (3200 images) 89-0038-04
Thermal Paper, 40 rolls (8000 images) 89-0038-05
UVP Visi-White™ Converter Plate (21x26cm) 38-0191-01
UVP Visi-White™ Converter Plate (20x40cm) 38-0191-02
UVP Visi-Blue™Converter Plate (21x26cm) 38-0200-01
UVP Visi-Blue™Converter Plate (20x40cm) 38-0200-02
UVP Filter, 50mm, sq., SYBR Green and EGFP 38-0219-01
UVP Filter, 50mm, sq., Ethidium Bromide 38-0220-01
Technical Assistance
Analytik Jena offers technical support on all of its products. If there are questions about the product’s use,
operation or repair, please contact our offices at the locations below, or go to Analytik Jena’s web site and
click the Tech Support > BioImaging Systems.
If residing in North America, South
America, East Asia or Australia:
If residing in Europe, Africa, the Middle East
of Western Asia:
Call [phone], and ask for Customer
Service during regular business days,
between 7:00 am and 5:00 pm, PST.
Call [phone], and ask for Customer
Service during regular business days between
9:00 am and 5:30 pm.
E-mail your message to:
[email protected]
E-mail your message to: [email protected]
Fax Customer Service, and send it to (909)
946-3597
Fax Customer Service, and send it to: +44(0)
1223-420561
Write to: Analytik Jena 2066 W. 11th Street,
Upland, CA 91786 USA
Write to: Ultra-Violet Products Ltd
Unit 1, Trinity Hall Farm Estate, Nuffield Road,
Cambridge CB4 1TG UK

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Warranty
Analytik Jena's products are guaranteed to be free of defects in materials, workmanship and manufacture for
one (1) year from date of purchase. Consumable and disposable parts including, but not limited to tubes and
filters, are guaranteed to be free from defects in manufacture and materials for ninety (90) days from date of
purchase. Transilluminators carry a two (2) year warranty from date of purchase. If equipment failure or
malfunction occurs during the warranty period, AJ shall examine the inoperative equipment and have the
option of repairing or replacing any part(s) which, in the judgment of AJ, were originally defective or became
so under conditions of normal usage and service.
No warranty shall apply to this instrument, or part thereof, that has been subject to accident, negligence,
alteration, abuse or misuse by the end-user. Moreover, AJ makes no warranties whatsoever with respect to
parts not supplied by AJ or that have been installed, used and/or serviced other than in strict compliance
with instructions appearing in this manual.
In no event shall AJ be responsible to the end-user for any incidental or consequential damages, whether
foreseeable or not, including but not limited to property damage, inability to use equipment, lost business,
lost profits, or inconvenience arising out of or connected with the use of instruments produced by AJ. Nor is
AJ liable or responsible for any personal injuries occurring as a result of the use, installation and/or servicing
of equipment.
This warranty does not supersede any statutory rights that may be available in certain countries.
DigiDoc-It and VsionWorks are registered trademarks of Analytik Jena US.