Endress+Hauser Analytik Jena Biometra TOne 96 User manual

Operating Manual
Biometra TOne
High-performance thermal cycler for
DNA amplification by PCR

Biometra GmbH (Analytik Jena AG)
Rudolf-Wissell-Str. 30
37079 Göttingen Germany
Phone + 49 551 50 68 6-0
Fax + 49 551 50 68 6-66
Email [e-mail]
Biometra GmbH
Rudolf-Wissell-Str. 30
37079 Göttingen Germany
Phone + 49 551 50 68 6 -12, -14 or -16
Fax + 49 551 50 68 6-11
Email [e-mail]
http://www.analytik-jena.com
Operating manual valid from software version ME 1.70 –RE 1.46
Microsoft and Windows are registered trademarks of Microsoft Corp.
The identification with ® or TM is omitted in this manual.
34-3000-000-23
03.19
Analytik Jena AG
© Copyright 2019, Analytik Jena AG
Manufacturer
Service
General information
Copyrights and
Trademarks
Documentation number
Edition
Implementation of the
Technical Documentation

Contents
Biometra TOne Manual Version 03_19 1
Contents
1Basic information...............................................................................................................5
1.1 Notes on the operating manual.................................................................................5
1.2 Purpose.....................................................................................................................6
1.1 Warranty and liability.................................................................................................6
2Safety and warning instructions........................................................................................7
2.1 General notes............................................................................................................7
2.2 Safety markings on the Biometra TOne....................................................................7
2.3 Technical condition ...................................................................................................7
2.4 Requirements for the operating personnel ...............................................................8
2.5 Safety instructions: transport and installation ...........................................................8
2.6 Safety instructions: during operation.........................................................................8
2.6.1 General..............................................................................................................8
2.6.2 Safety instructions: protection against explosion and fire.................................9
2.6.3 Safety instructions: electrical equipment...........................................................9
2.6.4 Handling of samples, auxiliary and operating materials ...................................9
2.7 Safety instructions: service and repair....................................................................10
2.8 Behavior during emergencies.................................................................................10
3Technical specifications..................................................................................................11
4Function and setup..........................................................................................................13
4.1 High speed thermoblock .........................................................................................13
4.2 High Performance Smart Lid...................................................................................14
4.3 Display ....................................................................................................................14
4.4 User-specific quick start of programs......................................................................14
4.5 Easy programming..................................................................................................14
4.6 Linear Gradient Tool ...............................................................................................15
4.7 GLP compliance......................................................................................................15
4.8 Open system philosophy.........................................................................................15
4.9 Order numbers........................................................................................................16
5Installation.......................................................................................................................17
5.1 Scope of delivery.....................................................................................................17
5.2 Unpacking and inspection.......................................................................................17
5.3 Setting the operating voltage..................................................................................17
5.4 Commissioning........................................................................................................18
6Control elements.............................................................................................................19
6.1 Biometra TOne front view .......................................................................................19
6.2 Biometra TOne rear view........................................................................................20
6.3 High Performance Smart Lid (HPSL)......................................................................20
6.3.1 Closing the lid..................................................................................................20

Contents
2 Version 03_19 Biometra TOne Manual
6.3.2 Opening the lid ................................................................................................21
6.4 The Biometra TOne software..................................................................................22
6.4.1 Keyboard.........................................................................................................22
6.4.2 Keypad ............................................................................................................23
6.4.3 Cursor keys.....................................................................................................23
6.4.4 Confirm or Cancel ...........................................................................................23
6.4.5 List of commands and icons used...................................................................24
7Operation.........................................................................................................................29
7.1 Power on self-test ...................................................................................................29
7.2 Login screen............................................................................................................29
7.3 Login........................................................................................................................30
7.4 Home screen...........................................................................................................31
7.5 Block status.............................................................................................................32
7.6 Quick start function .................................................................................................33
7.7 Incubation mode......................................................................................................34
8Creating, editing and saving programs...........................................................................36
8.1 Programming modes...............................................................................................36
8.2 Creating a new program/using a program template................................................37
8.3 Assigning program names ......................................................................................39
8.4 Setting the heated lid temperature..........................................................................39
8.5 Preheating the heated lid ........................................................................................40
8.6 Editing steps............................................................................................................40
8.6.1 Editing all parameters of a step.......................................................................41
8.6.1.1 Programming loops.....................................................................................42
8.6.1.2 Programming a temperature increment ......................................................43
8.6.1.3 Programming a time increment...................................................................43
8.6.1.4 Adjusting the ramp rate...............................................................................44
8.6.1.5 Programming a gradient step......................................................................44
8.6.2 Direct programming of program parameters for a step...................................46
8.7 Inserting a step........................................................................................................49
8.8 Deleting a step ........................................................................................................49
8.9 Saving the program.................................................................................................50
9Starting, coping and deleting programs ..........................................................................52
9.1 Starting a program ..................................................................................................52
9.2 Copying a program..................................................................................................53
9.3 Copying all programs..............................................................................................54
9.4 Deleting a program..................................................................................................55
9.5 Deleting all programs..............................................................................................56
10 Running, pausing, continuing and stopping programs....................................................57
10.1 Display during a run ................................................................................................57

Contents
Biometra TOne Manual Version 03_19 3
10.2 Pausing a program..................................................................................................59
10.3 Continuing the program ..........................................................................................60
10.4 Skipping a step........................................................................................................60
10.5 Stopping the program..............................................................................................60
11 Tools ...............................................................................................................................61
11.1 Configuration...........................................................................................................62
11.1.1 Setting the date and time................................................................................63
11.1.2 Automatic user logout .....................................................................................64
11.1.3 Configuring the beeper....................................................................................65
11.1.4 Network...........................................................................................................65
11.1.4.1 Network settings..........................................................................................65
11.1.4.2 Network users.............................................................................................66
11.1.5 Display brightness...........................................................................................66
11.1.6 Screen calibration ...........................................................................................67
11.1.7 Factory settings...............................................................................................68
11.2 User management...................................................................................................68
11.2.1 Creating a user................................................................................................69
11.2.2 Editing user settings........................................................................................70
11.2.3 Delete user......................................................................................................70
11.3 Documentation........................................................................................................71
11.3.1 Run log file ......................................................................................................72
11.3.2 Power On Logfile.............................................................................................74
11.3.3 Extended self-test log file................................................................................75
11.3.4 Error log file.....................................................................................................76
11.4 Extended self-test ...................................................................................................77
11.5 Service info file (SINF)............................................................................................79
11.6 Backup ....................................................................................................................80
11.7 Cycler Info...............................................................................................................81
11.8 Contact....................................................................................................................82
12 Adaptation of programs...................................................................................................83
13 Quick reference guide.....................................................................................................85
14 Troubleshooting ..............................................................................................................89
14.1 Administrator password forgotten...........................................................................89
14.2 Slow heating and cooling........................................................................................89
14.3 Auto restart..............................................................................................................89
14.4 Auto restart without an apparent cause..................................................................89
14.5 Adaptation of programs from other thermal cyclers................................................89
14.6 Loosening the lid wheel in case of blockage ..........................................................90
15 Maintenance and care ....................................................................................................91
15.1 Cleaning the housing ..............................................................................................91

Contents
4 Version 03_19 Biometra TOne Manual
15.2 Disinfecting the device ............................................................................................92
15.3 Firmware update .....................................................................................................92
16 Service ............................................................................................................................93
16.1 Device return information........................................................................................93
16.2 Packing the Biometra TOne....................................................................................94
17 Disposal...........................................................................................................................95
18 Declaration of conformity ................................................................................................96
19 Index................................................................................................................................97

Basic information
Biometra TOne Manual Version 03_19 5
1 Basic information
1.1 Notes on the operating manual
The Biometra TOne is intended for operation by qualified specialist personnel observing this
operating manual.
The operating manual contains information about the design and operation of the Biometra
TOne and provides personnel familiar with PCR technology with the necessary know-how for
the safe handling of the equipment and its components. The operating manual further
includes notes on the maintenance and service of the device and potential causes and
remedies of any faults.
Conventions
Instructions for action which occur in chronological order are numbered and combined into
action units and furnished with the corresponding results.
Lists which are not in chronological order are shown as itemized lists, sub-listings as bullet
points.
Safety instructions are indicated by pictographs and signal words. The type and source of
the danger are stated together with notes on preventing the danger. The meaning of the
pictographs and signal words used is explained in the chapter "Safety instructions".
Symbols and signal words used
The operating manual uses the following symbols and signal words to indicate hazards or
instructions. The safety instructions are always placed before an action.
WARNING
Indicates a potentially hazardous situation which, if not prevented, can
result in death or most serious injuries (incapacitation).
CAUTION
Indicates a potentially hazardous situation which, if not prevented, can
result in light or minor injuries and material damage.
ATTENTION
Indicates a potentially hazardous situation which, if not avoided, can result
in damage to the product or items in its vicinity.
Symbol for waste disposal in accordance with WEEE directive:
Do not dispose of in household waste
CE marking
China RoHS marking

Basic information
6 Version 03_19 Biometra TOne Manual
1.2 Purpose
The Biometra TOne is an end-point thermal cycler. It is designed to amplify nucleic acids by
repeated cycles of heating and cooling using DNA polymerases in a PCR reaction.
The thermal cycler is developed for Research Use Only (RUO).
The Tone combines modern design with user-friendly software. The user interface consists
of a touchscreen with a graphical or spreadsheet display that shows the time, the status and
the temperature program for each run. Using the touchscreen keyboard, information and
program parameters can be entered directly on the screen. Due to the high ramp rates, the
TOne thermal cycler is suitable for fast PCR applications, thereby helping to shorten
program run times.
1.1 Warranty and liability
The warranty duration and liability comply with the legal requirements and the provisions in
the general terms and conditions of Biometra.
Deviations from the intended use described in this manual result in limitations of warranty
and liability in the event of damage.
Warranty and liability claims are excluded for personal injury and property damage due to
one or several of the following causes:
•use of the Biometra TOne other than intended
•improper commissioning, operation and servicing of the device
•modifications of the Biometra TOne without prior consultation with Biometra GmbH
•unauthorized intervention in the Biometra TOne
•operation of the Biometra TOne with faulty safety equipment
•use of other than original spare parts, wearing parts or consumables
•improper repairs
•improper transport and storage of the Biometra TOne
•faults due to the non-observance of this manual

Safety and warning instructions
Biometra TOne Manual Version 03_19 7
2 Safety and warning instructions
2.1 General notes
For your own safety and to ensure error-free and safe operation of the Biometra TOne,
please read this chapter carefully before commissioning.
Besides the safety instructions in this user manual and the local safety regulations that apply
to the operation of the device, the general applicable regulations regarding accident
prevention, occupational health and safety and environmental protection have to be
observed and complied with.
References to potential dangers do not replace the work protection regulations which must
be observed.
2.2 Safety markings on the Biometra TOne
Damaged or missing safety symbols can cause incorrect actions leading to personal injury or
material damage. Do not remove safety symbols! Replace damaged safety symbols
immediately!
The following safety symbols are attached to the inside of the heated lid and the rear of the
device:
CAUTION! Hazard area!
Proceed with due care when handling samples and working with the Biometra
TOne.
CAUTION! Risk of burns!
There is a risk of burning at the heated lid, the thermal block, the samples, and the
rear of the device.
The following sign is attached to the underside of the Biometra TOne:
ATTENTION! Keep ventilation slits clear!
Keep the ventilation slits on the underside and at the rear of the
device clear. Make sure that there are no objects underneath the
device (e.g., paper) that may be sucked against the ventilation
slot and thus interfere with ventilation.
2.3 Technical condition
The Biometra TOne has been built and certified according to safety standard EN 61010-1.
Do not modify the device in any way. Any modification of the device will lead to a loss of
warranty and the EN 61010-1 certificate and poses a potential risk.
The following has to be observed:
❑The operator must only operate the device in a sound and operationally safe condition.
The technical condition must always comply with the legal requirements and
regulations.

Safety and warning instructions
8 Version 03_19 Biometra TOne Manual
❑Prior to every use the device must be checked for damage and sound condition.
❑Any changes in the device affecting its safety must be reported by the operating
personnel to the operator without delay.
2.4 Requirements for the operating personnel
The Biometra TOne must only be operated by qualified specialist personnel instructed in the
use of the device. The instructions also include imparting the contents of this manual.
In addition to the safety at work instructions in this operating manual the generally applicable
safety and accident prevention regulations of the respective country of operation must be
observed and adhered to. The operator must ascertain the latest version of these
regulations.
The operating manual must be accessible to the operating and service personnel at all
times.
2.5 Safety instructions: transport and installation
The following has to be observed:
❑Drain the sample block before transporting the Biometra TOne. Make sure there are no
sample tubes in the block.
❑Only ship the Biometra TOne in its original packaging with the transport lock installed.
❑Please verify that the delivery is complete on receipt of the Biometra TOne and check
for possible transport damage. In case of claims please contact Biometra GmbH.
2.6 Safety instructions: during operation
2.6.1 General
The operator of the Biometra TOne must make sure before each commissioning that the
condition of the device including the safety equipment is sound.
The following has to be observed:
❑Free access to the power switch on the back of the enclosure has to be ensured during
operation.
❑The ventilation fittings at the rear of the device must be unobstructed and operational.
Covered ventilation grilles or slits etc. may cause the device to break down or may
cause damage to it.
❑The use of oil between the samples and the sample block is not necessary to achieve
an improved heat exchange. However, if you still want to use oil, you should use
mineral oil. Do not use silicone oil.
❑Be careful to avoid crushing or pinching injuries when closing the device.
The Biometra TOne may cause burns. The following has to be observed:

Safety and warning instructions
Biometra TOne Manual Version 03_19 9
❑The thermal block, the samples and the heated lid reach high temperatures. There is a
risk of burns during contact.
❑Ensure that the lid is securely closed before starting the program! Do not touch the
heated lid!
❑The rapid heating of the thermal block can cause liquids to boil explosively. Always
wear safety goggles during operation!
❑Do not touch hot sample tubes or plates and do not open them or boiling liquid may
escape!
❑Only use plates and tubes that are suitable for high temperatures (up to 100°C), fit well
into the thermal block (no shaking) and whose lids seal tightly!
2.6.2 Safety instructions: protection against explosion and fire
The Biometra TOne must not be operated in an explosive environment.
The Biometra TOne must not be operated with flammable, explosive or volatile substances.
2.6.3 Safety instructions: electrical equipment
Work on the electrical components of the Biometra TOne may only be performed by a
qualified electrical technician in accordance with the applicable electrical engineering rules.
Life-threatening electrical voltages may occur in the interior of the Biometra TOne!
The following has to be observed:
❑Any work on the interior of the device may only be carried out by the customer service
of Biometra GmbH and specially authorized technicians.
❑The electrical components must be checked regularly by a qualified electrician. Any
defects, such as loose connections, faulty or damaged cables, must be repaired without
delay.
❑Before opening the device it must be switched off at the power switch and the mains
plug must be disconnected from the mains outlet!
❑The Biometra TOne must be switched off immediately using the power switch (on the
equipment backplate) and the mains plug disconnected from the power supply if any
faults occur in the electric components.
❑Do not operate the device near sources of strong electromagnetic radiation (for
example, an unshielded, deliberately operated high frequency source), as these may
affect proper operation of the device.
2.6.4 Handling of samples, auxiliary and operating materials
The operator is responsible for the selection of substances used in the process as well as for
their safe handling. This is particularly important for radioactive, pathogenic, infectious,
poisonous, corrosive or otherwise dangerous substances. For details contact the safety
officer responsible for your location. When handling dangerous substances local safety
codes and guidelines must be observed. The following general notes do not replace the
specific local regulations or the regulations in the EC safety data sheets of the manufacturers
for the auxiliary and operating materials.

Safety and warning instructions
10 Version 03_19 Biometra TOne Manual
The following has to be observed:
❑Protective goggles and rubber gloves have to be worn when handing reagents.
❑If only a few samples are treated, an (empty) tube of the same height must additionally
be placed at each corner position of the block. If the number of samples in the block is
too low, there is a risk of the tubes being damaged and sample liquid leaking out.
❑For your own safety, please observe the potential infectious qualities of the examined
biological material.
2.7 Safety instructions: service and repair
The Biometra TOne is usually repaired by the service department of Biometra GmbH or its
authorized and trained specialist personnel. Unauthorized repairs can damage the device.
Therefore, the operator may generally only carry out the tasks listed in chapter "Maintenance
and care" p. 91.
The following has to be observed:
❑The exterior of the device may only be cleaned with a damp, not dripping, cloth after the
device has been switched off.
❑Do not use alcohol (e.g., methanol or ethanol), organic solvents or abrasives to clean
the device.
❑Any maintenance on the device may usually only be carried out in the switched-off
condition (unless stated otherwise).
2.8 Behavior during emergencies
In case of danger or accidents, immediately switch off the Biometra TOne using the main
switch on the equipment backplate. Disconnect the mains plug from the power supply!

Technical specifications
Biometra TOne Manual Version 03_19 11
3 Technical specifications
Order number
846-x-070-311
846-x-070-301
Name
Biometra TOne 96
Biometra TOne 96G
Capacity
96 x 0.2 ml tubes, 96 well micro plates or 8 well strips
Block material
Aluminum
Block coating
Special alloy
Max. heating rate*
4.0 °C/sec
Max. cooling rate*
3.3 °C/sec
Avg. heating rate*
3.7 °C/sec
Avg. cooling rate*
3.0 °C/sec
Max./min gradient
-
20 °C/0.1 °C
Gradient temperature range1
-
20 °C to 99 °C
Temperature uniformity
95°C
+/- 0.60 °C after 15 s
72°C
+/- 0.30 °C after 15 s
55°C
+/- 0.20 °C after 15 s
Temperature range
3 °C to 99 °C
Control accuracy
+/- 0.1 °C
Software
User-specific quick start option for the five most recent
programs; program preview before start; option for
toggling between programming table and graph
programming mode; Linear Gradient Tool1, service info
file (SINF) generation; expanded self-test; adjustable
ramp rates; gradient temperature diagram view; Ethernet-
based PC control via PCR Control App and
tablet/smartphone with Android/iOS
Program memory
Total capacity of 350 programs in up to 90 user
directories
Language
English, German, Chinese
Display
7" color touchscreen
Automatic restart after power failure
Yes
High Performance Smart Lid (HPSL)
technology
Yes
Lid temperature range
30 °C to 110 °C

Technical specifications
12 Version 03_19 Biometra TOne Manual
Power consumption
550 watt
Operating voltage
100, 115, 230 Volt, 50 - 60 Hz
Noise emissions
Very low, max. 45 dB
Interfaces1
USB A, Ethernet
Environmental conditions
15 °C to 35 °C, 70% air humidity, max. 2,000 m above
sea level
Dimensions (WxDxH)
260 mm x 430 mm x 210 mm
Dimensions (WxDxH) with open lid
260 mm x 430 mm x 385 mm
Weight
11.5 kg
*measured inside the block
1The network cable must be at least performance class Cat 5e and the cable configuration has to be
STP
x = 2 for 230 V, 4 for 115 V, 5 for 100 V

Function and setup
Biometra TOne Manual Version 03_19 13
4 Function and setup
The Biometra TOne thermal cycler is available in the following models:
•Biometra TOne 96 with aluminum block
•Biometra TOne 96G with aluminum block and gradient function
The device is controlled by a 7'' touchscreen display and an easy-to-use user interface. PCR
protocols and run log files can be saved to a connected USB stick. By using the USB
functions, PCR protocols can be exchanged easily between devices and run log files can be
stored for documentation reasons on a PC.
Fig. 1 The Biometra TOne Thermal Cycler
The Biometra TOne thermal cycler features an automatic restart. If a power failure occurs
during the run, the device will continue the run as soon as power is restored. In case of long-
term power failure (longer than 30 minutes), the device will keep the sample block at 4°C
(freeze step) and the user can decide to repeat the run with the same samples or to discard
them.
The block with gradient function can optionally be used for the optimization of new primer
pairs. The Linear Gradient Tool allows the programming of gradients with a defined
temperature difference between the rows or columns of the sample block.
This manual provides information on how to use all models of the Biometra TOne Thermal
Cycler most effectively. Functions that are only available for certain versions are indicated
accordingly, for example, "This function is only available for gradient-enabled thermal
cyclers".
4.1 High speed thermoblock
All blocks of the Biometra TOne thermal cycler are made of aluminum and offer high ramp
rates for fast protocol run times. A rubber seal on the heated lid forms an encapsulated
space around the sample block as soon as the lid is closed. The closed space serves to
improve the temperature uniformity of the sample block and avoids the formation of
condensed water at the final PCR cooling step. Furthermore all blocks are perfectly sealed to
prevent condensed water from penetrating into the Peltier elements underneath the sample
block and other parts of the electronics. The sealing protects the Peltier elements and
prolongs the lifetime of the device.

Function and setup
14 Version 03_19 Biometra TOne Manual
4.2 High Performance Smart Lid
The Biometra TOne heated lid has been optimized to heat twice as fast as with older models
and thus helps to shorten protocol run times. Moreover the heated lid fulfills two other
important functions: It prevents the formation of condensation at the reaction tube portion
located above the block surface level and it ensures reliable contact between the reaction
tubes and the thermoblock by applying constant pressure. Thanks to the integrated clutch
mechanism, the pressure applied to the reaction tubes is always the same, regardless of the
plasticware height. The combination of the heated lid shape and the reliable contact
pressure ensures even temperature distribution between samples, thus significantly
improving temperature uniformity.
With one press on the front button, the heated lid gently swings open and locks in its end
position. This mechanism ensures that the lid cannot fall down and the heated lid is out of
the radius of action while the user inserts PCR tubes or plates. This prevents any risk of
injury from crushing or hot surfaces.
4.3 Display
The Biometra TOne thermal cycler features a 7" state-of-the-art color touchscreen user
interface. The touchscreen is built-in at a flat angle to ensure reflection-free viewing and
ergonomic programming. For programming PCR protocols the software incorporates
Biometra's proven spreadsheet philosophy and alternatively offers graphical programming.
One touch of a button leads from the spreadsheet to the alternative graphical programming
mode. This makes the creation of new programs or editing existing programs fast and easy.
4.4 User-specific quick start of programs
Many other thermal cyclers offer a so-called "List of latest programs used". In the list of latest
programs used a certain number of PCR programs is collected and offered for quick start.
The list is typically ordered only by date and the user has to search for the desired program.
In contrast the Biometra TOne software creates a user-specific list and only the latest
programs started by the currently logged-in user are offered for quick start. Even if a lab
member does not use the device for a longer time, the information on the latest programs
started by this user will not get lost.
For maximum convenience the Biometra TOne software additionally features a program
preview. Before the start of a program the protocol steps are summarized in a clearly
arranged table by the program preview tool. The preview therefore provides a
comprehensive overview of the protocol structure without the need to access programming
screens. The program preview is also available before the quick start of programs.
4.5 Easy programming
Creating new PCR programs takes a lot of time if for each single step all parameters have
to be set manually. The Biometra TOne software offers several pre-installed program
templates for different applications. The program templates provide a general protocol
structure that can be easily adapted for the current experiment.

Function and setup
Biometra TOne Manual Version 03_19 15
4.6 Linear Gradient Tool
For optimizing new primer pairs in a single run the Biometra TOne can optionally be
equipped with a gradient function. Finding the best primer annealing temperature is crucial
for the specificity and efficiency of PCR reactions. Often the optimization of experiments is
conducted to a limited extent only leading to unspecific byproducts or reduced PCR
sensitivity. By using the gradient function, new primer pairs with unknown annealing
temperatures can be tested quickly and optimized in a very short time.
For maximum convenience the Biometra TOne offers the Linear Gradient Tool. For most
other devices the temperature difference from row to row or from column to column in a
gradient step varies across the sample block. The Biometra TOne Linear Gradient Tool
enables the programming of gradient steps with a defined temperature increment between
the columns or rows. The Linear Gradient Tool option allows the comfortable creation of
gradient steps and thus the best annealing temperature can be easily transformed to a non-
gradient protocol.
4.7 GLP compliance
The software can manage up to 30 user accounts. With each system start the Biometra
TOne performs an initial self-test and additionally the software offers an extended self-test
that can be initiated by the user. The results of the extended self-test are summarized in a
protocol and stored by the device. In addition to the self-test functions, the device creates
and stores log files for each single run. For long-term storage, the log files and extended
self-test protocols can be exported as a proprietary file format.
4.8 Open system philosophy
The Biometra TOne is an open system regarding consumables and reagents. Depending on
the sample block, the system can be used with single tubes, strips or plates. PCR plates can
be standard profile or low profile and they can be skirted, half-skirted or non-skirted. The
Biometra TOne can handle all kinds of plates and the plasticware be sealed with domed lids,
flat lids, sealing foil or other technologies. Regardless of the sealing method used, thanks to
the HPSL technology (see chapter 4.2) the same pressure is always applied to the
consumables for absolutely reproducible conditions.
The Biometra TOne thermal cycler is not limited to reagents from a specific vendor and can
be used with any kind of polymerase. Regardless of whether isothermal, hotstart or non-
hotstart polymerases are used, the Biometra TOne will produce reliable results. By
programming a pause step for the initial denaturation, for example, the system can be used
for manual hotstart applications. After inserting the samples into the heating block, the PCR
protocol can be started just by pressing "continue".

Function and setup
16 Version 03_19 Biometra TOne Manual
4.9 Order numbers
Instruments incl. block and base unit
Model
Block type
Gradient range
Order number
TOne 96
Aluminum
-
846-x-070-311
TOne 96G
Aluminum
20°C
846-x-070-301
Abbreviations:
G = gradient
x = 2 for 230 V, 4 for 115 V, 5 for 100 V

Installation
Biometra TOne Manual Version 03_19 17
5 Installation
5.1 Scope of delivery
1. Thermal cycler
2. Mains cable
3. Operating instructions
4. Quick reference guide
Please keep the original packaging in case the device needs to be returned. The shipping
box for the Biometra TOne thermal cycler contains a specially developed foam system for
the transport of high quality electronic devices.
5.2 Unpacking and inspection
Unpack and carefully check the device. Immediately report any damage to Biometra GmbH.
Do not attempt to operate the device if there is any visible damage.
Keep the original packaging for possible return. The Biometra TOne must always be
transported in its original packaging. If the packaging is no longer available, please contact
Biometra GmbH.
5.3 Setting the operating voltage
WARNING
Danger of electric shock!
Before commissioning the device, make sure that the voltage selector setting on the
underside of the device corresponds to the mains voltage in your laboratory.
The Biometra TOne can be operated at 100, 115 or 230 volts.
❑Set the operating voltage by turning the dial on the underside of the Biometra TOne to
the desired voltage. You can do so with a coin.
Image 1 Voltage selector switch on the underside of the device

Installation
18 Version 03_19 Biometra TOne Manual
5.4 Commissioning
WARNING
Danger of electric shock!
Prior to commissioning, please make sure that the operating voltage set on the device is the
same as the mains voltage (see section "Setting the operating voltage" p. 17).
Attention
Do not cover the ventilation slits!
Ensure that the ventilation slits are clear. Insufficient ventilation may cause the device to
overheat.
•Make sure that there are no objects underneath the device (e.g., paper) that may
be sucked against the ventilation slot and thus interfere with ventilation.
•Place the device at a minimum distance of 10 cm between the rear of the device
and the wall or other objects.
•In case of several cyclers side by side running simultaneously we would
recommend to keep a distance of at least 10 cm between every thermal cycler.
❑Place the Biometra TOne on a solid dry surface.
❑If the Biometra TOne has been moved, allow the device to adjust to room temperature
before switching it on for the first time (1 to 6 hours).
❑Connect the Biometra TOne to a grounded mains outlet using the mains cable supplied.
Make sure that the mains switch and the mains cable are easily accessible. This is
important if the device needs to be disconnected from the mains voltage.
❑The display contrast can be adjusted according to the respective lighting conditions (see
section "Display brightness" p. 66).