Endress+Hauser analytikjena Biometra TRIO How to use

Short Manual Biometra TRIO
Analytik Jena GmbH
Konrad-Zuse-Strasse 1
07745 Jena ⋅ Germany
Phone [phone]
[email protected]
www.analytik-jena.com
Short Manual Biometra TRIO
Version: A (07/2022)
34-3600-003-23
1/2
Safety instructions
Only use the quick reference guide in combination with the detailed operating manual.
WARNING!Risk of electric shock! Check whether the voltage selector switch (bottom) is
set to the correct mains voltage.
CAUTION!Risk of burns! Close the heated lid before starting the program. Use protective
equipment when handling hot samples.
NOTICE!Reduce the contact pressure for opening: Turn the wheel counterclockwise and
then press the button. After closing the lid, apply the contact pressure via the lid wheel.
1
2
3
4
Design
¡Wheel for exerting the contact pressure(1)
¡Lid sealing button(2)
¡Touch screen display with control software(3)
¡USB port(4)
Login and Home screen
After the initial self test, the software first opens the login screen and then the Home screen.
Option Icon Description
Login Logging in a user
Quick Start Block Display of the current block status. If the block is free:
Tap to open the quick-start function
DE/EN/CN /¡Change login screen language
¡Set the software language in the user management
Programs ¡Create or edit PCR programs
¡Select a program and start the PCR run
Protocol Wizard ¡Automatically create a PCR program
¡Calculate the annealing temperature and holding
time for the elongation step
Incubate Incubate the samples at constant temperature
Tools ¡Customize the software settings
¡Set up the user management
¡View documentation, self test
¡Create Service info file, carry out a backup
Free
Quick Start
Programs Incubate Tools
Logout
28.0°C
Block 1
User: John Doe
dd.mm.yyyy hh:ss
Protocol Wizard
Quick Start
28.0°C
Free
Quick Start
28.0°C
Free
Block 2 Block 3
Creating or editing PCR programs
}Tap Programs on the Home screen.
}Select a program on the page Program overview and edit it. Or: Tap New from tem-
plate. Select a program template and tap Open template to open it.
}Use or to select the graphical or the spreadsheet programming mode.
Name: Lid: Preheat Lid: On
Home Edit Insert Delete Done Back
Step
Go To
Cycles
∆T
∆t
∆R
30x
1 2 3 4 5 6Step Step Step Step Step
Go To
Temperature [°C]
Three-step
dd.mm.yyyy hh:mm
Program Runtime: 1h 15m 10s
User: John Doe
Name: Lid: Preheat Lid: On
Home Edit Insert Delete Done Back
Loops Step Block Temp. (°C)
Hold Time
(h:mm:ss) Go To Cycles ∆T(°C) ∆t(s) ∆R(°C/s)
Three-step
dd.mm.yyyy hh:mm
Program Runtime: 1h 15m 10s
User: John Doe
Graphical programming mode Spreadsheet programming mode
}Enter the program name under Name:. Define the temperature for the heated lid under
Lid:. Select On to activate the preheating function of the heated lid.
}Use Insert and Delete to add or delete steps. Maximum number of steps: 30
}Select a program step for editing. Change individual parameters in graphical/spreadsheet
view. Or: Tap Edit and change all parameters.
}Define the block temperature (3to99°C) and the holding time (max. ≤9h59min59s).
Use ∞ to program a pause.
}If required, define optional program parameters.
}Tap Done. Select a user directory under Copy/save programs.
}Save the program. Or: Enter a new name under Save as.

Short Manual Biometra TRIO
Analytik Jena GmbH
Konrad-Zuse-Strasse 1
07745 Jena ⋅ Germany
Phone [phone]
[email protected]
www.analytik-jena.com
Short Manual Biometra TRIO
Version: A (07/2022)
34-3600-003-23
2/2
Setting optional program parameters
}Open a program or a program template for editing. Select a step.
}Program loops: Define to which step the program shall return under GoTo. Define the
number of reiterations under Cycles. Maximum: 999
}Define a temperature increment (±0.1to20°C) under ΔT by which the temperature shall
increase/decrease per cycle.
}Define a time increment (1to240s) under Δt by which the holding time shall increase
per cycle.
}Set the heating and cooling rate precisely in steps of 0.1°C/s under ΔR.
}In order to optimize the annealing temperature, define the temperature optimization
step TOS in which the block temperature shall increase or decrease from one block to the
next, see below.
}If prompted, confirm your entries. Tap Done. Tap Save to save the program.
Programming a temperature optimization step
Annealing Temp.:
Increment:
Block 1 Block 2 Block 3
Step30x 2
Step GoTo
}Open a program or a program template for editing. Select the annealing step.
}Tap the field for the block temperature. Tap TOS on the keypad. Or: In the graphical/
spreadsheet view, first tap Edit and then TOS.
}Enter the annealing temperature under Annealing Temp.:. The temperature is applied to
block2.
}Define a temperature increment under Increment: which determines the temperatures
for block1 and block3.
}Confirm your entries. Tap Done. Tap Save to save the program.
Copying and deleting programs
Option Icon Description
Copy all Copy all programs of the selected user
Copy Copy the selected program
Delete all Delete all programs of the selected user
Delete Delete the selected program
Starting, stopping and pausing programs
}Use the quick-start function on the login screen/Home screen, to start the PCR run.
}Or: Tap Programs on the Home screen. Select the user directory and the program on the
page Program overview.
User: Admin dd.mm.yyyy hh:mm
Home EditDelete
Program overview
New from
template
Copy
User
users3
USB
Admin
John Doe
Programs
in directory3 Preview
Step
Step
GoTo
Program information
Name
Block Type:
Lid:
Program Runtime:
Three-step-da
99 °C Preheat Lid: On
01h 14m 48s
30x 2
48 well Program Type TOS
Start TOS-
Programm
}Use the arrow keys to navigate through the list, if the number of users/programs is
greater than what can be displayed.
Or: Use to display all users/programs.
}Use the preview function to validate the program and tap Edit to adjust it, if required.
}Insert samples into the thermal block. Use replicates of a sample to optimize the anneal-
ing temperature. Close the lid. Establish the contact pressure: Turn the wheel clockwise
until you hear a clicking sound.
}Tap Start to start the program.
}Select one or several blocks in the prompt Please select block to start:. Confirm your se-
lection.
}Use Start TOS-Programm to start a program with TOS. The program will start automati-
cally and simultaneously on all blocks.
}Tap Pause to stop the currently executed program. Tap Continue to resume the program.
Tap Skip to skip a step. Tap Stop program to abort a PCR run.
}Tap Choose block stop program to select one or several blocks.